Cellulase efficient immobilization device

Abstract

The utility model relates to the technical field of bioengineering, in particular to an efficient cellulase immobilization device which comprises a reaction tank body, a seal head arranged at the top of the reaction tank body and a seal bottom arranged at the bottom of the reaction tank body, a drip tube, a feed port and an exhaust port are arranged on the seal head, a discharge port is arranged on the seal bottom, and a jacket is arranged on the outer wall of the reaction tank body. A heat preservation layer is arranged on the outer wall of the jacket, a guide rail is arranged in the reaction tank body, a pulley is slidably mounted on the guide rail, a driving motor is mounted on the pulley, the instillation pipe penetrates through the end socket and extends downwards, the liquid outlet end of the instillation pipe is connected to the pulley, and the pulley is used for driving the liquid outlet end of the instillation pipe to reciprocate along the guide rail. According to the utility model, efficient immobilization treatment can be carried out on free cellulase, the liquid outlet end of the instillation tube can reciprocate on the guide rail at a constant speed along with the pulley, and the instillation speed of the instillation tube is uniform and stable, so that the prepared gel beads for immobilizing cellulase are uniform in texture, size and shape.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, in particular to a high-efficiency cellulase immobilization device. Background Art

[0002] Cellulose is an abundant and cheap renewable resource in nature, accounting for 40% to 60% of biomass raw materials. However, cellulose is almost insoluble in water and most conventional organic solvents, which seriously hinders the hydrolysis of cellulose and makes it difficult to utilize cellulose. At present, the industrial cellulose degradation process generally adopts harsh acid hydrolysis, but strong acid will corrode equipment and pollute the environment. With the change of green and sustainable development concepts, bio-enzymatic cellulose hydrolysis has received more and more attention due to its energy saving and environmental protection. This method uses the composite cellulase produced by microorganisms to act on the β-1,4-glycosidic bond of cellulose to degrade cellulose into glucose, thereby effectively utilizing cellulose.

[0003] In the process of enzymatic hydrolysis of cellulose by biological methods, since cellulase is a complex enzyme system, the direct use of free cellulase has problems such as low cellulase utilization, impure products, unstable reactions, and difficulty in enzyme recovery, which leads to high production costs and affects the industrial application of cellulase. An effective way to solve the problem of cellulase application is to screen highly active cellulase and immobilize it, so that the activity of cellulase is stable and reusable, the activity and effective utilization of cellulase are improved, and the cost of enzyme use is reduced. Compared with free cellulase, immobilized cellulase has higher selectivity and better specificity, can meet the needs of industrial production, can be reused, has good stability, and has low enzyme activity loss. Utility Model Content

[0004] In view of the technical problems existing in the prior art, the purpose of the utility model is to provide a high-efficiency cellulase immobilization device, through which immobilized cellulase gel can be efficiently prepared, the liquid outlet end of the drip tube can follow the pulley to move on the guide rail, and sodium alginate cellulase liquid can be dripped at different positions of the reaction tank to form immobilized cellulase gel beads, thereby avoiding gel accumulation and affecting gel formation; the dripping speed of the drip tube can be adjusted by a dripping speed regulator, so that the texture, size and shape of the obtained immobilized cellulase gel beads are uniform.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A high-efficiency cellulase immobilization device comprises a reaction tank body, a sealing head arranged on the top of the reaction tank body, and a bottom seal arranged on the bottom of the reaction tank body, wherein the sealing head is provided with a dripping tube, a feeding port and an exhaust port, the bottom seal is provided with a discharging port, the outer wall of the reaction tank body is provided with a jacket, the outer wall of the jacket is provided with a heat-insulating layer, a guide rail is provided inside the reaction tank body, a pulley is slidably mounted on the guide rail, a driving motor is mounted on the pulley, the dripping tube passes through the sealing head and extends downward, the liquid outlet end of the dripping tube is connected to the pulley, and the pulley is used to drive the liquid outlet end of the dripping tube to reciprocate along the guide rail.

[0007] Furthermore, the drip tube is fixedly installed at the center of the end cap, and the liquid inlet end of the drip tube is located above the end cap.

[0008] Furthermore, a dripping speed regulator is also provided on the dripping tube for adjusting the dripping speed of the dripping tube.

[0009] Furthermore, it also includes a DCS control system, which is electrically connected to the drive motor and the drip speed regulator.

[0010] Furthermore, the guide rail is arranged above the interior of the reaction tank body along the radial direction of the reaction tank body.

[0011] Furthermore, a movable filter screen is arranged on the bottom cover, and the discharge port is located below the movable filter screen.

[0012] Furthermore, a first communication port is provided at the upper portion of the jacket, and a second communication port is provided at the lower portion of the jacket.

[0013] Furthermore, a supporting foot is fixedly connected to the bottom cover.

[0014] Furthermore, the drip tube is made of soft silicone.

[0015] Furthermore, the reaction tank body is cylindrical, and the head and bottom are hemispherical.

[0016] In general, the utility model has the following advantages:

[0017] The utility model can immobilize free cellulase, and the liquid outlet end of the drip tube can follow the pulley to move on the guide rail, and the sodium alginate cellulase liquid is dripped at different positions of the reaction tank body to form immobilized cellulase gel beads, thereby avoiding gel accumulation and affecting gel formation; the dripping speed of the drip tube can be adjusted by a dripping speed regulator, so that the obtained immobilized cellulase gel beads have uniform texture, size and shape. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the utility model.

[0019] Among them, 1 is the reaction tank body, 2 is the head, 201 is the feed port, 202 is the exhaust port, 3 is the bottom cover, 301 is the movable filter, 302 is the discharge port, 4 is the jacket, 401 is the first connecting port, 402 is the second connecting port, 5 is the insulation layer, 6 is the guide rail, 7 is the drip tube, 701 is the liquid inlet end of the drip tube, 702 is the liquid outlet end of the drip tube, 703 is the drip speed regulator, 8 is the pulley, and 9 is the supporting foot. DETAILED DESCRIPTION

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0021] like Figure 1 As shown, a high-efficiency cellulase immobilization device comprises a reaction tank body, a head arranged on the top of the reaction tank body, and a bottom cover arranged at the bottom of the reaction tank body, wherein a drip tube, a feed port and an exhaust port are arranged on the head cover, specifically, the drip tube is fixedly installed at the center position of the head cover, the liquid inlet end of the drip tube is located above the head cover, a discharge port is arranged on the bottom cover, a supporting foot is fixedly connected to the bottom cover, a jacket is arranged on the outer wall of the reaction tank body, a heat-insulating layer is arranged on the outer wall of the jacket, a guide rail is arranged inside the reaction tank body, specifically, the guide rail is arranged above the inside of the reaction tank body along the radial direction of the reaction tank body, a pulley is slidably installed on the guide rail, a driving motor is installed on the pulley, the drip tube passes through the head cover and extends downward, the liquid outlet end of the drip tube is connected to the pulley, and the pulley is used to drive the liquid outlet end of the drip tube to reciprocate along the guide rail.

[0022] like Figure 1 As shown, the drip tube is also provided with a drip speed regulator for adjusting the drip speed of the drip tube. The drip tube is made of soft silicone.

[0023] The utility model of the cellulase efficient immobilization device also includes a DCS control system, which is electrically connected to a driving motor and a dripping speed regulator. The dripping speed regulator on the dripping tube is controlled by the DCS control system, so that the dripping tube can achieve uniform and quantitative liquid discharge; the driving motor is controlled by the DCS control system, so that the pulley can move at a uniform speed on the guide rail; the pulley drives the liquid outlet end of the dripping tube to move on the guide rail, so that the dripping tube can drip evenly in different places in the reaction tank body.

[0024] like Figure 1 As shown, the upper part of the jacket is provided with a first connecting port, and the lower part of the jacket is provided with a second connecting port. By providing the jacket on the outer wall of the reaction tank body, the reaction tank body can be heated or cooled. Specifically, when the temperature needs to be increased during the reaction process, high-temperature steam is introduced into the first connecting port, and condensed water is discharged from the second connecting port to achieve temperature increase; when the temperature needs to be reduced during the reaction process, cooling water is introduced into the second connecting port, and cooling water is discharged from the first connecting port to achieve temperature reduction.

[0025] like Figure 1 As shown, a movable filter is provided on the bottom cover, and the discharge port is located below the movable filter. When the reaction product is solid, the movable filter can discharge the reaction waste liquid first, leaving the solid product inside the reaction tank body, and the solid product can be discharged from the discharge port at the bottom of the reaction tank body by removing the movable filter.

[0026] In this embodiment, the reaction tank body is cylindrical, and the head and bottom are hemispherical.

[0027] The operating steps of the utility model are as follows:

[0028] (1) First, 50 mL of cellulase solution was measured and added to 450 mL of sodium alginate solution with a mass fraction of 4%, and then 5 mL of glutaraldehyde solution with a mass fraction of 1% was added, mixed evenly, and flowed into the reaction tank from the feed port of the device. High-temperature steam was introduced into the jacket from the first connecting port to raise the temperature of the reaction tank to 65° C. The temperature was kept constant for 2 hours, and the obtained sodium alginate cellulase solution was discharged from the discharge port for use.

[0029] (2) A 6% calcium chloride solution is flowed into the reaction tank from the feed port, and the prepared sodium alginate cellulase solution is passed into the drip tube and dripped into the calcium chloride solution in the reaction tank at a rate of 30 ml / min. Immobilized cellulase gel beads are generated instantly when the sodium alginate cellulase solution contacts the calcium chloride solution.

[0030] During the dripping process, the DCS control system controls the driving motor and the dripping speed regulator, thereby controlling the liquid outlet end of the drip tube to follow the pulley to move at a uniform speed on the guide rail and drip at a uniform speed, so that the liquid outlet end of the drip tube can drip sodium alginate cellulase solution at different positions of the reaction tank body to form immobilized cellulase gel beads, so that the immobilized cellulase gel beads can be evenly dispersed in the reaction tank body, avoiding gel accumulation and affecting gel formation, so that the obtained immobilized cellulase gel beads have uniform texture, size and shape.

[0031] (3) After the dripping is completed, cooling water is introduced into the jacket from the second connecting port to reduce the temperature of the reaction tank to 20°C, the discharge port is opened, and the movable filter screen intercepts the immobilized cellulase gel beads in the reaction tank body, and the solution in the reaction tank body is discharged from the discharge port. Then, a 4°C calcium chloride solution is added from the feed port, and the solution is left to harden for 1 hour. The immobilized cellulase gel beads are left in the reaction tank body again, the solution is discharged, and then washed with a sodium chloride solution with a mass fraction of 0.9% for 3 times, and then rinsed with distilled water for 2 times to obtain the final immobilized cellulase gel beads. Then the movable filter screen is removed, and the immobilized cellulase gel beads in the reaction tank body are discharged from the discharge port. Use absorbent paper to absorb the moisture on the surface of the beads and store them in a refrigerator at 4°C for use.

[0032] In general, the utility model can carry out efficient immobilization treatment on the screened free cellulase, and control the driving motor on the pulley and the dripping speed regulator on the dripping tube through the DCS control system, so that the pulley can drive the liquid outlet end of the dripping tube to reciprocate at a uniform speed on the guide rail, and can also make the dripping speed of the dripping tube uniform and stable, so that the prepared gel beads of immobilized cellulase have uniform texture, size and shape, higher quality, stable activity and can be reused, thereby meeting the needs of industrial production and reducing the cost of enzyme use.

[0033] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A highly efficient cellulase immobilization device, characterized in that: It includes a reaction tank body, a head cover arranged on the top of the reaction tank body, and a bottom cover arranged at the bottom of the reaction tank body, the head cover is provided with a drip tube, a feed port and an exhaust port, the bottom cover is provided with a discharge port, the outer wall of the reaction tank body is provided with a jacket, the outer wall of the jacket is provided with a heat-insulating layer, the interior of the reaction tank body is provided with a guide rail, a pulley is slidably installed on the guide rail, a driving motor is installed on the pulley, the drip tube passes through the head cover and extends downward, the liquid outlet end of the drip tube is connected to the pulley, and the pulley is used to drive the liquid outlet end of the drip tube to move back and forth along the guide rail.

2. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The drip tube is fixedly installed at the center of the end cap, and the liquid inlet end of the drip tube is located above the end cap.

3. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The drip tube is also provided with a drip speed regulator for adjusting the drip speed of the drip tube.

4. The high-efficiency cellulase immobilization device according to claim 3, characterized in that: It also includes a DCS control system, which is electrically connected to the driving motor and the drip speed regulator.

5. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The guide rail is arranged above the interior of the reaction tank body along the radial direction of the reaction tank body.

6. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: A movable filter screen is arranged on the bottom cover, and the discharge port is located below the movable filter screen.

7. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The upper part of the jacket is provided with a first communication port, and the lower part of the jacket is provided with a second communication port.

8. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: A supporting foot is fixedly connected to the bottom cover.

9. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The drip tube is made of soft silicone.

10. The high-efficiency cellulase immobilization device according to claim 1, characterized in that: The reaction tank body is cylindrical, and the head and bottom are hemispherical.

Images