A cross-linking instrument
By designing a combination of high-sealing reaction chamber and multi-wavelength ultraviolet lamp unit in the crosslinker, the problems of poor sealing and large wavelength span of traditional optical crosslinking equipment are solved, and more efficient and stable biomolecular crosslinking fixation are achieved.
Patent Information
- Application Number
- CN202420550189.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-20
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2034-03-20
AI Technical Summary
Traditional photocrosslinking equipment has poor sealing properties, and crosslinking substances are prone to react with active gases, resulting in poor crosslinking effect or failure of crosslinking. At the same time, the wavelength span of the ultraviolet lamp tube is large and the crosslinking time is long.
A crosslinker is designed with a high-sealing reaction chamber, equipped with an exhaust port and an inert gas input port to prevent the accumulation of active gas in the reaction chamber. At the same time, multiple ultraviolet lamp units with different wavelengths are adopted, arranged on the top of the reaction chamber in an array, providing a narrow band light source to improve crosslinking efficiency.
The reaction between crosslinking substances and active gas is effectively avoided, the sealing and efficiency of crosslinking is improved, the crosslinking time is shortened, and the stability of crosslinking effect is improved.
Smart Images
Figure CN222719257U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a cross-linking instrument, and more specifically, to a cross-linking instrument. Background Art
[0002] In the process of high-throughput detection of biomolecular interactions, it is often necessary to immobilize multiple biomolecules, and the efficiency and method of their immobilization on the substrate will directly affect the biochemical detection.
[0003] The photocrosslinking fixation method is fast and gentle. On the one hand, it can quickly covalently fix biomolecules to make them firmly coupled; on the other hand, it can fix biomolecules in random orientations to ensure that active sites are not blocked due to fixation, thereby retaining their detection activity.
[0004] However, the sealing of traditional photo-crosslinking equipment is poor. At the same time, during the photo-crosslinking reaction, the crosslinking material easily reacts with the active gas, resulting in poor crosslinking effect or crosslinking failure. In addition, the photo-crosslinking reaction mainly uses ultraviolet lamps to react, mostly using 320nm~400nm and 275nm~320nm bands, with a large wavelength span and a long crosslinking time. Utility Model Content
[0005] The embodiment of the utility model provides a cross-linking apparatus, which has high airtightness and can effectively avoid the problem of poor cross-linking effect or cross-linking failure caused by the reaction between the cross-linking material and the active gas.
[0006] An embodiment of the utility model also provides a cross-linking instrument, comprising: a box body, the box body is provided with a reaction chamber with an opening, and a hatch that can open or seal the opening, the cross-linking instrument also includes at least one light-emitting unit arranged in the box body and irradiating the reaction chamber; the box body is also provided with an exhaust port and an inert gas input port connected to the reaction chamber, the exhaust port is configured to discharge the air in the reaction chamber to form a negative pressure, and the inert gas input port is configured to supply inert gas into the reaction chamber after the air in the reaction chamber is discharged.
[0007] In an exemplary embodiment, the light-emitting units include a plurality of light-emitting units, the plurality of light-emitting units include a first ultraviolet lamp unit and a second ultraviolet lamp unit, and the wavelengths of the first ultraviolet lamp unit and the second ultraviolet lamp unit are different.
[0008] In an exemplary embodiment, the wavelength range of the first ultraviolet lamp unit and the second ultraviolet lamp unit is: 200-400nm.
[0009] In an exemplary embodiment, the first ultraviolet lamp unit and the second ultraviolet lamp unit each include a plurality of them, and the plurality of the first ultraviolet lamp units and the plurality of the second ultraviolet lamp units are arranged in an array on the top of the reaction chamber.
[0010] In an exemplary embodiment, the cross-linking apparatus further includes an exhaust device connectable to the exhaust port, and a gas supply device connectable to the inert gas input port.
[0011] In an exemplary embodiment, the reaction chamber and the housing are designed as an integral unit or assembled separately.
[0012] In an exemplary embodiment, the housing is provided with an installation space isolated from the reaction chamber, the light emitting unit is provided in the installation space, and the housing is further provided with a light-transmitting portion for allowing light from the light emitting unit to enter the reaction chamber.
[0013] In an exemplary embodiment, the light-transmitting portion is a prismatic plate.
[0014] In an exemplary example, the cross-linking instrument also includes a controller disposed in the housing and electrically connected to the at least one light-emitting unit and a control panel electrically connected to the controller, wherein the controller is configured to control the operation of the light-emitting unit according to a control command received by the control panel; and the controller is disposed on a side wall of the housing located in the installation space.
[0015] In an exemplary embodiment, the cross-linking instrument further includes a reflective plate disposed on the inner side of the reaction chamber or a reflective material coated on the inner wall of the reaction chamber.
[0016] In an exemplary embodiment, the side wall of the box body is provided with a plurality of heat dissipation holes connecting the installation space with the outside.
[0017] Compared with the related art, the cross-linking apparatus of the embodiment of the utility model is provided with a sealable reaction chamber in the casing, which has high airtightness. At the same time, by providing an exhaust port and an inert gas input port connected to the reaction chamber, the active gas in the reaction chamber can be removed before the object is cross-linked, thereby avoiding the problem of poor cross-linking effect or cross-linking failure caused by the reaction of the cross-linking substance with the active gas.
[0018] Other features and advantages of the utility model will be described in the following description, and partly become apparent from the description, or understood by implementing the utility model. The purpose and other advantages of the utility model can be realized and obtained through the structures particularly pointed out in the description and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] The accompanying drawings are used to provide a further understanding of the technical solution of the utility model and constitute a part of the specification. Together with the embodiments of the utility model, they are used to explain the technical solution of the utility model and do not constitute a limitation on the technical solution of the utility model.
[0020] Figure 1This is an overall three-dimensional view of the cross-linking instrument of an embodiment of the utility model;
[0021] Figure 2 for Figure 1 Another angle view of
[0022] Figure 3 This is a disassembled view of a cross-linking apparatus according to an embodiment of the utility model;
[0023] Figure 4 It is a perspective view of a cross-linking apparatus according to an embodiment of the present utility model. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical solution and advantages of the utility model more clear, the embodiments of the utility model will be described in detail with reference to the accompanying drawings. It should be noted that the embodiments and features in the embodiments of the utility model can be combined with each other arbitrarily without conflict.
[0025] like Figure 1-Figure 4 As shown, the embodiment of the present invention provides a cross-linking apparatus 100 that can be used to fix biomolecules.
[0026] like Figure 1 As shown, the cross-linking instrument 100 comprises a housing 1, which is provided with a reaction chamber 10 having an opening 101 and a door 11 which can open or seal the opening 101. The door 11 is attracted to the housing 1 by magnetic attraction 3.
[0027] like Figure 4 As shown, the cross-linking instrument 100 also includes at least one light-emitting unit 12 disposed in the housing 1 and irradiating the reaction chamber 10. The light-emitting unit 12 provides light to achieve cross-linking and fixation of the biomolecules in the reaction chamber 10. Figure 2 As shown, the housing 1 is also provided with an exhaust port 13 and an inert gas input port 14 connected to the reaction chamber 10. The exhaust port 13 is configured to exhaust the air in the reaction chamber 10 to form a negative pressure, and the inert gas input port 14 is configured to supply inert gas to the reaction chamber 10 after the air in the reaction chamber is exhausted. When cross-linking is performed, the exhaust port 13 and the inert gas input port 14 can be blocked by a blocking member 131. The plurality of light-emitting units 12 are LED light-emitting units and are provided on the LED integrated board 4.
[0028] The cross-linking apparatus 100 of the embodiment of the utility model is provided with a sealable reaction chamber 10 in a housing 1, and has high airtightness. Meanwhile, by providing an exhaust port 13 and an inert gas input port 14 connected to the reaction chamber 10, the active gas in the reaction chamber 10 can be removed before cross-linking of the object, thereby avoiding the problem of poor cross-linking effect or cross-linking failure caused by the reaction between the cross-linking molecules and the active gas during cross-linking.
[0029] like Figure 4As shown, the light emitting unit 12 in the embodiment of the utility model includes a plurality of light emitting units 12, and the plurality of light emitting units 12 include a first ultraviolet lamp unit 121 and a second ultraviolet lamp unit 122, and the wavelengths of the first ultraviolet lamp unit 121 and the second ultraviolet lamp unit 122 are different.
[0030] Conventional ultraviolet lamp equipment needs to switch to another wavelength for reaction after irradiation with ultraviolet light of one wavelength in a dark environment, and photodegradation will occur during the transfer process. The embodiment of the utility model can complete the irradiation reaction of different wavelengths in the same reaction chamber by setting a light-emitting unit 12 of multiple wavelengths, eliminating the photodegradation of the sample during the transfer process, simplifying the operation, and avoiding the need to replace multiple devices to avoid photodegradation during the transfer process.
[0031] In an exemplary embodiment, the wavelength range of the first ultraviolet lamp unit 121 is: 200-400nm, and the wavelength range of the second ultraviolet lamp unit 122 is: 200-400nm. Generally, common wavelengths are 254, 320, 365, 366, 385, 395, and 405nm. Exemplarily, the wavelength of the first ultraviolet lamp unit 121 can be 320nm, and the wavelength of the second ultraviolet lamp unit 122 can be 365nm. Specifically, different wavelengths can be achieved by replacing modular lamp groups according to different usage requirements.
[0032] Conventional broadband equipment requires 15-20 minutes of irradiation for 10 mM small molecule DMSO using an ordinary UV lamp crosslinker. However, the crosslinker 100 of the present invention uses a narrow-band wavelength of the first UV lamp unit 121 and the second UV lamp unit 122, so the light irradiation energy is more concentrated and the reaction time is shorter (generally about 5 minutes), which greatly saves time cost.
[0033] like Figure 3 As shown, the first ultraviolet lamp unit 121 and the second ultraviolet lamp unit 122 each include a plurality, and the plurality of first ultraviolet lamp units 121 and the plurality of second ultraviolet lamp units 122 are arranged in an array on the top of the reaction chamber 10, thereby improving the uniformity of illumination of each light-emitting unit.
[0034] The cross-linking apparatus 100 of the embodiment of the utility model further includes an exhaust device (not shown) that can be connected to the exhaust port 13, and a gas supply device (not shown) that can be connected to the inert gas input port 14. The exemplary exhaust device can be a vacuum pump, and the gas supply device can be a gas supply bottle containing inert gas. The inert gas can be nitrogen or argon.
[0035] like Figure 3 , Figure 4As shown, the housing 1 is provided with an installation space 15 isolated from the reaction chamber 10, and the light-emitting unit 12 is provided in the installation space 15. The housing 1 is also provided with a light-transmitting portion 16 for the light of the light-emitting unit 12 to enter the reaction chamber 10. Among them, the light-transmitting portion adopts a high-density prism plate. The cross-linking instrument 100 of the embodiment of the utility model can ensure the high airtightness of the reaction chamber 10 by arranging the light-emitting unit 12 in the installation space 15 isolated from the reaction chamber 10, and then inputting the light into the reaction chamber 10 through the light-transmitting portion 16. At the same time, the installation difficulty of the light-emitting unit 12 is reduced. The housing 1 includes an outer shell 1a, an inner cavity 1b and an upper cover 1c, and the inner cavity 1b and the upper cover 1c are enclosed to form a storage space. The housing 1 is also provided with a light-transmitting plate 5, and the light-transmitting plate is provided with a light-transmitting portion 16, and the light-transmitting plate 5 isolates the reaction chamber 10 and the installation space 15 from each other.
[0036] The reaction chamber 10 and the housing 1 can be of an integrated design or a split design. When the reaction chamber 10 and the housing 1 are of a split design, the reaction chamber 10 can be placed in the housing 10 or taken out of the housing 10, so that the cleaning of the reaction chamber 10 can be facilitated on the one hand, and a variety of reaction chambers 10 can be selected to be placed in the housing 10 to adapt to different cross-linking substances on the other hand.
[0037] like Figure 3 , Figure 4 As shown, the cross-linking instrument 100 of the embodiment of the utility model further includes a controller 17 disposed in the housing 1 and electrically connected to at least one light-emitting unit 12, and a control panel 18 connected to the controller 17, wherein the controller 17 is configured to control the light-emitting unit 12 to work according to a control command received by the control panel 18; the controller 17 is disposed on a side wall of the housing 1 located in the installation space 15. The control panel 18 can display the status of the reaction chamber 10, such as reaction time, type of reaction light source, and the like.
[0038] The cross-linking instrument 100 of the embodiment of the present invention further includes a reflective plate 102 disposed on the inner side of the reaction chamber 10 or a reflective material coated on the inner wall of the reaction chamber 10. The reflective plate 102 or the reflective material coated on the inner wall of the reaction chamber 10 can increase the light efficiency of the light emitting unit 12 and further shorten the reaction time.
[0039] like Figure 4 As shown, the side wall of the box 1 of the cross-linking instrument 100 of the embodiment of the utility model is provided with a plurality of heat dissipation holes 103 connecting the installation space 15 with the outside, which can dissipate heat for the light-emitting unit 12 and improve the service life of the reflective unit without affecting the airtightness of the reaction chamber.
[0040] like Figure 1As shown, the door 11 of the cross-linking instrument 100 of the embodiment of the utility model is provided with a handle 110 and an observation window 111. The user can open or close the door through the handle and observe the reaction inside through the observation window 111. The door 11 can be provided with a sealing ring (not shown) to ensure the sealing of the door 11 and the opening 101.
[0041] like Figure 2 As shown, the top of the box body 1 is provided with a top cover 104 enclosing the installation space 15, and a plurality of light-emitting units 12 are installed at the bottom of the top cover 104. The box body 1 is also provided with a power interface 20, which can be connected to a 220V cross-link power supply.
[0042] In the description of the present invention, it should be noted that the terms "upper", "lower", "one side", "the other side", "one end", "the other end", "side", "relative", "four corners", "periphery", "mouth-shaped structure" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the structure referred to has a specific orientation, is constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0043] In the description of the embodiments of the present utility model, unless otherwise clearly specified and limited, the terms "connection", "direct connection", "indirect connection", "fixed connection", "installation", and "assembly" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; the terms "installation", "connection", and "fixed connection" can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
[0044] Although the implementation methods disclosed in the utility model are as above, the above contents are only implementation methods adopted to facilitate the understanding of the utility model, and are not used to limit the utility model. Any technician in the field to which the utility model belongs can make any modifications and changes in the form and details of implementation without departing from the spirit and scope disclosed in the utility model, but the scope of patent protection of the utility model shall still be defined by the attached claims.
Claims
1. A cross-linking instrument, characterized in that: include: A box body, wherein the box body is provided with a reaction chamber with an opening and a hatch that can open or seal the opening, and the cross-linking instrument also includes at least one light-emitting unit arranged in the box body and irradiating the reaction chamber; the box body is also provided with an exhaust port and an inert gas input port connected to the reaction chamber, the exhaust port is configured to allow the air in the reaction chamber to be discharged to form a negative pressure, and the inert gas input port is configured to allow the inert gas to be input into the reaction chamber after the air in the reaction chamber is discharged.
2. The cross-linking apparatus according to claim 1, characterized in that: The light emitting units include a plurality of light emitting units, including a first ultraviolet lamp unit and a second ultraviolet lamp unit, and the wavelengths of the first ultraviolet lamp unit and the second ultraviolet lamp unit are different.
3. The cross-linking apparatus according to claim 2, characterized in that: The wavelength range of the first ultraviolet lamp unit and the second ultraviolet lamp unit is: 200-400nm.
4. The cross-linking apparatus according to claim 2, characterized in that: The first ultraviolet lamp unit and the second ultraviolet lamp unit each include a plurality of them, and the plurality of the first ultraviolet lamp units and the plurality of the second ultraviolet lamp units are arranged in an array manner on the top of the reaction chamber.
5. The cross-linking apparatus according to claim 1, characterized in that: It also includes an exhaust device that can be connected to the exhaust port, and a gas supply device that can be connected to the inert gas input port.
6. The cross-linking apparatus according to claim 1, characterized in that: The reaction chamber and the box body are designed as an integral unit or assembled in a separate unit.
7. The cross-linking apparatus according to any one of claims 1 to 6, characterized in that: The box body is provided with an installation space which is isolated from the reaction chamber, the light emitting unit is arranged in the installation space, and the box body is further provided with a light-transmitting portion for light of the light emitting unit to enter the reaction chamber.
8. The cross-linking apparatus according to claim 7, characterized in that: The light-transmitting portion is a prism plate.
9. The cross-linking apparatus according to claim 7, characterized in that: It also includes a controller disposed in the box and electrically connected to the at least one light-emitting unit and a control panel electrically connected to the controller, wherein the controller is configured to control the operation of the light-emitting unit according to a control command received by the control panel; the controller is disposed on a side wall of the box located in the installation space.
10. The cross-linking apparatus according to claim 7, characterized in that: It also includes a reflective plate arranged on the inner side of the reaction chamber or a reflective material coated on the inner wall of the reaction chamber.
11. The cross-linking apparatus according to claim 7, characterized in that: The side wall of the box body is provided with a plurality of heat dissipation holes connecting the installation space with the outside.