A sample purification kit for vitamin B12
By designing the vitamin B12 fast sample purification kit and fully automatic sample pre-processing instrument, the sample pre-processing steps are simplified, the cumbersome pre-processing problems in existing detection methods are solved, and the rapid and efficient detection results are achieved.
Patent Information
- Application Number
- CN202420617298.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-28
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2034-03-28
AI Technical Summary
In the existing vitamin B12 detection methods, the processing steps before sample detection are cumbersome, which makes the preparation work before the test time-consuming and laborious, and the rapid detection cannot be achieved.
A rapid sample purification kit for vitamin B12 is designed, including sample tube position, bead tube position, cleaning tube position and acquisition tube position. The sample pretreatment process is simplified by optimizing the kit structure and using fully automatic sample pretreatment instruments.
It realizes rapid pretreatment of vitamin B12 sample detection, improves detection efficiency, saves manpower, and ensures consistency of the test results.
Smart Images
Figure CN222719259U_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of biological detection, and in particular to a vitamin B12 sample purification kit. Background Art
[0002] Vitamin B12 (VB12) is the only essential water-soluble vitamin containing metal elements. It is involved in the synthesis of methionine, two metabolic functions that are essential for normal cell growth and DNA synthesis, and the conversion of methylmalonyl CoA to succinyl CoA. It plays an important role in the metabolism of substances in the body. When the body is deficient in it, symptoms such as megaloblastic anemia, leukopenia and thrombocytopenia will appear, potentially inducing a variety of neurological diseases.
[0003] Vitamin B12 is an important test item in infant food and dairy products. The commonly used detection methods include chemiluminescence and enzyme-linked immunosorbent assay. However, due to the cumbersome pre-treatment steps of existing samples, the preparation work before the test is time-consuming and laborious. Therefore, it is still necessary to develop a rapid sample purification kit for vitamin B12. Summary of the invention
[0004] The technical problem to be solved by the present invention is to provide a rapid sample purification kit for vitamin B12, which simplifies the preparation process of reagents before detection and saves detection time.
[0005] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows.
[0006] A vitamin B12 rapid sample purification kit comprises a long strip-shaped kit body (10), on which different functional tube positions (20) are arranged, and the openings of the functional tube positions (20) are arranged above the kit body (10), and the functional tube positions are respectively a sample tube position (1), a magnetic bead tube position (2), a cleaning tube position (3), and a collection tube position (4).
[0007] Preferably, the sample tube position is used to place initial sample extraction, and the capacity of the sample tube position is 50-2000 microliters.
[0008] Preferably, the magnetic bead tube is used to place magnetic beads, and the capacity of the magnetic bead tube is 100-1000 microliters.
[0009] Preferably, the cleaning tube has at least two sub-cleaning sections, and the sub-cleaning sections include at least one reaction hole and at least one cleaning hole.
[0010] Preferably, the cleaning pipe position may also include a spare sub-cleaning section, including a reaction hole and a cleaning hole.
[0011] Preferably, the cleaning tube position further comprises a reserved sample dilution hole (5).
[0012] Preferably, the cleaning tube position is used to place cleaning liquid, and the capacity of the cleaning tube position is 100-2000 microliters.
[0013] Preferably, the test tube position is used to collect standard samples after pre-processing, and the capacity of the test tube position is 10-1000 microliters.
[0014] Preferably, the reagent kit body is provided with a handle (30) for easy handling.
[0015] Preferably, the surface of the kit is covered with a sealing film (6) when not in use.
[0016] Beneficial effects:
[0017] 1. The present invention can achieve pre-treatment of vitamin B12 sample detection by optimizing the rapid sample purification kit of vitamin B12. The sample purification kit is used in conjunction with a fully automatic sample pre-treatment instrument, which can not only improve the efficiency of food detection and save manpower, but also ensure the reliability and stability of the sample pre-treatment process to ensure the consistency of the detection results. The present invention has a simple structure and is easy to use, and can effectively improve the detection efficiency;
[0018] 2. The number and position of holes in the kit can be selected as needed, which improves the utilization rate and versatility of the kit. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 A rapid sample purification kit for vitamin B12 BRIEF DESCRIPTION OF THE DRAWINGS 10-reagent box; 20-functional tube position; 30-handle; 1-sample tube position; 2-magnetic bead tube position; 3-cleaning tube position; 4-collection tube position; 5-reserved sample dilution hole; 6-sealing film; 21-reserved magnetic bead tube position; 31-reserved cleaning tube position
[0021] It should be understood that the accompanying drawings are not drawn to scale, but are only appropriately simplified drawings for illustrating various features of the basic principles of the present invention. The specific design features of the present invention disclosed herein include, for example, specific dimensions, directions, positions and shapes that will be determined in part by the specific environment in which they are to be applied and used. DETAILED DESCRIPTION
[0022] Example 1 A rapid sample purification kit for vitamin B12
[0023] The first embodiment of the present application relates to a rapid sample purification kit for vitamin B12, as shown in the attached Figure 1The reagent box is in the shape of a long strip, and different functional tube positions 20 are arranged on the reagent box body 10, and the opening of the functional tube position 20 is arranged above the reagent box, and the functional tube positions 20 are respectively a sample tube position 1, a magnetic bead tube position 2, a cleaning tube position 3, a collection tube position 4, a reserved sample dilution hole 5, and a sealing film 6.
[0024] Among them, the sample tube position is used to place the initial sample extraction, and the capacity of the sample tube position is 50-2000 microliters.
[0025] Among them, the magnetic bead tube capacity is 100-1000 microliters.
[0026] Wherein, the cleaning tube has at least two sub-cleaning sections, and the sub-cleaning sections include at least one reaction hole and at least one cleaning hole.
[0027] Among them, the cleaning tube position is used to place the cleaning solution, and the capacity of the cleaning tube position is 100-2000 microliters.
[0028] Among them, the collection tube capacity is 10-1000 microliters.
[0029] Among them, a handle is provided on the test kit body for easy holding.
[0030] The surface of the test kit is covered with a sealing film when not in use.
[0031] Example 2 Specific application of a rapid sample purification kit for vitamin B12
[0032] 1. Transfer 200uL of the initial dairy sample from the sample tube to the reaction tube;
[0033] 2. Mix the magnetic beads in the magnetic bead tube by pipetting evenly, use a pipette to draw the magnetic bead liquid into the pipette tip, place it near the magnet, and discard the supernatant after the magnetic beads are separated from the liquid;
[0034] 3. Discard the supernatant and transfer the magnetic beads to the reaction tube, mix the magnetic beads and the sample thoroughly, and react for 20 minutes to form a ternary complex of magnetic beads-coated vitamin B12 antibody-vitamin B12 antigen;
[0035] 4. Suck all the magnetic bead-liquid complex in the reaction tube into the gun tip. The magnet is close to the gun tip and absorbs the magnetic beads. After the magnetic beads are completely absorbed, spit out the liquid in the gun tip and leave the magnetic beads. Transfer the gun tip to the cleaning tube.
[0036] 5. Use the cleaning solution in the cleaning tube to mix the magnetic beads and liquid evenly. After the mixture is evenly mixed, keep the beating state for 2 minutes;
[0037] 6. Suck all the magnetic bead-liquid mixture in the cleaning tube into the gun tip. The magnet is close to the gun tip and absorbs the magnetic beads. After the magnetic beads are completely absorbed, spit out the liquid in the gun tip and leave the magnetic beads. Transfer the gun tip to the cleaning tube position 2.
[0038] 7. Repeatedly blow the gun tip in the cleaning tube to mix the magnetic beads and liquid evenly. After the mixture is evenly mixed, keep blowing for 2 minutes;
[0039] 8. Aspirate all the magnetic bead-liquid mixture in the cleaning tube into the gun tip, place the gun tip near the magnet and adsorb the magnetic beads. After the magnetic beads are completely adsorbed, remove the liquid in the gun tip and leave the magnetic beads, and transfer them to the collection tube;
[0040] 9. Transfer the liquid to the collection tube, mix it evenly by pipetting, and proceed with subsequent operations.
[0041] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model will have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.
Claims
1. A vitamin B12 sample purification kit, characterized in that: The kit comprises a strip-shaped reagent box body, on which different functional tube positions are arranged, and the openings of the functional tube positions are arranged above the reagent box. The functional tube positions are respectively a sample tube position, a magnetic bead tube position, a cleaning tube position, and a collection tube position.
2. A vitamin B12 sample purification kit according to claim 1, characterized in that: The sample tube position is used to place the initial sample for extraction, and the capacity of the sample tube position is 50-2000 microliters.
3. A vitamin B12 sample purification kit according to claim 1, characterized in that: The magnetic bead tube has a capacity of 100-1000 μl.
4. A vitamin B12 sample purification kit according to claim 1, characterized in that: The cleaning tube has at least two sub-cleaning sections, and the sub-cleaning sections include at least one reaction hole and at least one cleaning hole.
5. A vitamin B12 sample purification kit according to claim 1, characterized in that: The cleaning tube position also includes a reserved sample dilution hole.
6. A vitamin B12 sample purification kit according to claim 1, characterized in that: The cleaning tube position is used to place cleaning liquid, and the capacity of the cleaning tube position is 100-2000 microliters.