Dyeing device

Abstract

The utility model provides a dyeing device, which includes a first shell, a second shell, a first electrode plate and a second electrode plate; the first electrode plate is arranged on the first shell, the second electrode plate is arranged on the second shell, the first shell and the second shell can cover each other and form a receiving space between the first electrode plate and the second electrode plate; the first shell is provided with a first through hole, the first through hole is connected to the first electrode; the second shell is provided with a second through hole, the second through hole is connected to the second electrode. The first electrode plate is energized through the first through hole, and the second electrode plate is energized through the second through hole, so that an electric field is formed between the first electrode plate and the second electrode plate, so as to electrophores the biological material in the receiving space, so that the protein therein is combined with the dye. The dyeing device in the utility model has the advantages of simple structure, easy operation, low manufacturing cost, etc.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology and is a protein dyeing device with portability. Background Art

[0002] Protein staining refers to staining proteins with dyes, and then qualitatively and quantitatively detecting proteins and analyzing protein distribution. Protein staining under normal conditions is usually done by physical diffusion, and it takes many hours for the dye and protein to combine. Electrophoresis technology can greatly speed up the time for the dye and protein to combine. Electrophoresis technology is a technology that uses the different speeds of charged particles moving in an electric field to achieve separation effects, and achieves a uniform staining effect on proteins through a uniform electric field. Under certain conditions, the distance (i.e., mobility) that charged particles move per unit time under the action of unit electric field strength is a constant. Different charged particles have different charges, or have the same charge but different charge-to-mass ratios. After a certain period of time, they are electrophoresed in the same electric field and separated from each other due to different moving distances. The structure of existing biological staining devices is very complex, usually with oven specifications and sizes, and the cost is relatively high. Utility Model Content

[0003] In view of the deficiencies in the prior art, the utility model provides a staining device to achieve the purpose of a protein staining device with a simple structure and lower manufacturing cost.

[0004] To this end, in one embodiment, a dyeing device is provided, including a first shell, a second shell, a first electrode plate and a second electrode plate; the first electrode plate is arranged on the first shell, and the second electrode plate is arranged on the second shell, the first shell and the second shell can cover each other and form a receiving space between the first electrode plate and the second electrode plate; a first through hole is provided on the first shell, and the first through hole is connected to the first electrode; a second through hole is provided on the second shell, and the second through hole is connected to the second electrode.

[0005] As a further optional solution of the dyeing device, the first shell is provided with a positioning hole, and the second shell is provided with a positioning protrusion, and the positioning protrusion can be inserted into the positioning hole.

[0006] As a further optional solution of the dyeing device, the first shell and the second shell are hinged.

[0007] As a further optional scheme for the dyeing device, the first shell includes a first supporting member, and the second shell includes a second supporting member, the first supporting member is used to fix the first electrode plate, and the second supporting member is used to fix the second electrode plate; when the first shell and the second shell cover each other, the first supporting member and the second supporting member abut against each other, and the first electrode plate, the second electrode plate, the first supporting member and the second supporting member enclose the accommodation space.

[0008] As a further optional solution of the dyeing device, the first shell is provided with a liquid inlet hole, and the liquid inlet hole is connected to the receiving space to facilitate the injection of the dye.

[0009] As a further optional solution of the dyeing device, the second shell is provided with a liquid outlet, and the liquid outlet is connected to the receiving space to facilitate the discharge of the dye.

[0010] As a further optional solution for the dyeing device, an annular groove is provided at the covering portion of the first shell corresponding to the second shell, and the second shell is provided with an annular protrusion, and when the first shell and the second shell cover each other, the annular protrusion is inserted into the annular groove.

[0011] As a further optional solution of the dyeing device, the second shell is further provided with a third through hole, and the third through hole is connected to the accommodating space.

[0012] As a further optional solution of the dyeing device, the first shell and the second shell can cover each other and have a gap.

[0013] As a further optional solution for the dyeing device, a mounting portion is further provided on the first shell, and the mounting portion is used for hingedly fixing the dyeing device.

[0014] Implementing the embodiments of the present utility model will have the following beneficial effects:

[0015] According to the dyeing device in the above embodiment, the operator generally puts the dye and the biological material to be dyed into the receiving space. After the first shell and the second shell cover each other, a receiving space is formed between the first electrode plate and the second electrode plate. The first electrode plate is energized through the first through hole, and the second electrode plate is energized through the second through hole, so that an electric field is formed between the first electrode plate and the second electrode plate, thereby electrophoresing the biological material in the receiving space so that the protein therein is combined with the dye. The dyeing device in the utility model has the advantages of simple structure, easy operation, and low manufacturing cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the prior art descriptions. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0017] Figure 1 The overall structural schematic diagram of the dyeing device provided according to the embodiment of the utility model is shown;

[0018] Figure 2 Shows Figure 1 Schematic diagram of the cross section at the middle line AA;

[0019] Figure 3 Shows Figure 2 A partial enlarged view of the middle A;

[0020] Figure 4 The structure schematic diagram of the dyeing device provided by the embodiment of the utility model at a front view angle is shown;

[0021] Figure 5 The structure schematic diagram of the dyeing device provided by the embodiment of the utility model in a top view is shown;

[0022] Figure 6 A schematic diagram of the decomposed structure of a dyeing device provided according to an embodiment of the utility model is shown;

[0023] Figure 7 Shows Figure 6 A partial enlarged view of point B in the middle.

[0024] Description of main component symbols:

[0025] First shell-10; second shell-20; first electrode plate-30; second electrode plate-40;

[0026] Screen-90; receiving space-12; first through hole-110; second through hole-220; positioning hole-120;

[0027] Positioning protrusion-270; first supporting member-130; second supporting member-230; liquid inlet hole-140;

[0028] Liquid outlet hole-240; annular groove-150; annular protrusion-250; third through hole-160; notch-50;

[0029] Mounting portion-60; second protrusion-1310; third protrusion-2310. DETAILED DESCRIPTION

[0030] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively with reference to the accompanying drawings. The accompanying drawings provide preferred embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0031] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0033] In the embodiment of the present utility model, a dyeing device is provided. Please refer to Figure 1-Figure 6 The dyeing device includes a first shell 10, a second shell 20, a first electrode plate 30 and a second electrode plate 40; the first electrode plate 30 is arranged on the first shell 10, and the second electrode plate 40 is arranged on the second shell 20. The first shell and the second shell 20 can cover each other and form a receiving space 12 between the first electrode plate 30 and the second electrode plate 40; the first shell 10 is provided with a first through hole 110, and the first through hole 110 is connected to the first electrode; the second shell 20 is provided with a second through hole 220, and the second through hole 220 is connected to the second electrode.

[0034] According to the dyeing device in the above embodiment, the operator generally puts the dye and the biological material to be dyed into the receiving space 12. After the first shell 10 and the second shell 20 cover each other, the receiving space 12 is formed between the first electrode plate 30 and the second electrode plate 40. The first electrode plate 30 is energized through the first through hole 110, and the second electrode plate 40 is energized through the second through hole 220, so that an electric field is formed between the first electrode plate 30 and the second electrode plate 40, thereby electrophoresing the biological material in the receiving space 12 so that the protein therein is combined with the dye. The dyeing device in the utility model has the advantages of simple structure, easy operation, low manufacturing cost, etc.

[0035] In general, the dyeing material and biological material filled in the receiving space 12. The dyeing material can be one or more of organic dyes, inorganic dyes, fluorescent dyes, and composite dyes: organic dyes are one or more of Coomassie Brilliant Blue, Amido Black, Ponceau Red, Fast Green FCF, Zinc Reagent, and Chrome Black; inorganic dyes are one or more of copper ions, ferrous ions, and other heavy metal ions; fluorescent dyes are one or more of ethidium bromide, acridine dyes, and cyanine dyes; composite dyes are one or more of inorganic dyes and organic dyes, fluorescent dyes and organic dyes, and fluorescent dyes and inorganic dyes.

[0036] It should be noted that the dyeing device in the present invention is not limited to protein dyeing, and dyeing of other organic and inorganic substances is also within the protection scope of the present invention.

[0037] In the embodiment of the utility model, the first housing 10 is provided with a positioning hole 120 , and the second housing 20 is provided with a positioning protrusion 270 , and the positioning protrusion 270 can be inserted into the positioning hole 120 .

[0038] In this embodiment, the first shell 10 and the second shell 20 can be positioned so as to cover each other through the positioning hole 120 and the positioning protrusion 270 , thereby ensuring the sealing of the receiving space 12 .

[0039] For further information, please refer to Figure 6 , a second positioning protrusion 1310 may also be provided on the first supporting member 130, and the second positioning protrusion 1310 is inserted into the positioning hole 120 to achieve the positioning of the first supporting member 130 and the first housing 10. Then, a third positioning protrusion 2310 is provided on the second supporting member 230, and the third positioning protrusion 2310 can be inserted into the positioning hole 120 or into the hollow part of the second protrusion 1310. Finally, the first housing 10, the second housing 20, the first supporting member 130 and the second supporting member 230 can all be positioned and fixed.

[0040] In the embodiments of the present invention, please refer to Figure 6 and Figure 7 When the first shell 10 and the second shell 20 cover each other, the first shell 10 and the second shell 20 can be aligned by hinge connection, thereby ensuring that the receiving space 12 formed each time has the characteristic of consistency.

[0041] In a specific embodiment, the first housing 10 and the second housing 20 are hinged to each other, which is convenient for operators to use and will not cause the first housing 10 and the second housing 20 to be separated and lost.

[0042] It should be noted that when the first shell 10 and the second shell 20 cover each other, in order to prevent the first shell 10 and the second shell 20 from falling off, generally there is a damping structure at the hinge of the first shell 10 and the second shell 20. Through the design of the damping structure, the positions of the first shell 10 and the second shell 20 can be kept relatively unchanged.

[0043] In the specific embodiment, please refer to Figure 1 , 2, 3 and Figure 6 The first supporting member 130 includes a gauze 90, which is fixed to the first supporting member 130 in some way, and the gauze 90 is used to support the biological material, and the second supporting member 230 is used to fix the biological material; when the second supporting member 230 is assembled to the first supporting member 130, the first shell 10 and the second shell 20 cover each other, the first electrode plate 30, the second electrode plate 40, the first supporting member 130 and the second supporting member 230 enclose a receiving space 12.

[0044] The first electrode plate 30 and the second electrode plate 40 cannot be in contact with each other, and a certain distance must be maintained so that a stable uniform electric field is formed between the first electrode plate 30 and the second electrode plate 40. When the first shell 10 and the second shell 20 cover each other, the first supporting member 130 on the first shell 10 and the second supporting member 230 on the second shell 20 abut against each other to press the biomaterial and ensure that it is in the receiving space 12. The first supporting member 130 and the second supporting member 230 are generally frame-shaped to facilitate the assembly of the gauze 90, the placement of the biomaterial, and the full contact between the liquid and the biomaterial to avoid air bubble gaps between the biomaterials.

[0045] Of course, the first electrode plate 30 can also be fixed on the first housing 10, and the second electrode plate 40 can be fixed on the second housing 20. This is usually not done because the first electrode plate 30, the second electrode plate 40, the first housing 10 and the second housing 20 need to be regularly disassembled and cleaned.

[0046] In some specific embodiments, the first housing 10 is provided with a liquid inlet 140 , and the liquid inlet 140 is connected to the receiving space 12 to facilitate the injection of dye.

[0047] In order to facilitate the operation of the operator and speed up the time of dyeing the biological material, the biological material is first placed on the gauze 90, and then the second supporting member 230 is assembled; then the first shell 10 and the second shell 20 are closed. Then the dye is poured through the liquid inlet 140 until the dye fills the receiving space 12. In this way, the dye can fill the receiving space 12 and fully contact with the biological material, so as to achieve the purpose of dyeing the biological material as soon as possible.

[0048] In some specific embodiments, the second shell 20 is provided with a liquid outlet 240 , and the liquid outlet 240 is connected to the receiving space 12 to facilitate the discharge of the dye.

[0049] In this embodiment, the liquid outlet 240 is used to discharge the dye after the biological material is stained.

[0050] Of course, the liquid inlet 140 can be filled with dye or drained, but this usually leads to dye contamination.

[0051] In some specific embodiments, please refer to Figure 6 and Figure 7 The first shell 10 is provided with an annular groove 150 at the covering portion corresponding to the second shell 20 , and the second shell 20 is provided with an annular protrusion 250 . When the first shell 10 and the second shell 20 cover each other, the annular protrusion 250 is inserted into the annular groove 150 .

[0052] The annular protrusion 250 and the annular groove 150 are adapted to each other, and by squeezing the silicone strip in the annular groove 150 , a sealing effect can be achieved, so that the dye and biological material in the receiving space 12 are not easy to flow out.

[0053] Specifically, a sealing ring is provided in the annular groove 150. When the annular protrusion 250 is pressed into the sealing ring (ie, the silicone strip) in the annular groove 150, it can further ensure that the dyeing device in this embodiment does not leak.

[0054] In some specific embodiments, please refer to Figure 1 The second shell 20 is further provided with a third through hole 160 , and the third through hole 160 is connected to the receiving space 12 .

[0055] A sensor is assembled at the third through hole 160, which is used to detect the height of the liquid level when the device is placed vertically; the sensor is connected to the main circuit through a line, and this time it needs to be sealed from the outside, and the third through hole is connected to the receiving space.

[0056] The sensor is generally connected to a central control device, which is usually a control circuit board. The inflow and outflow of the dyeing liquid can be controlled by feedback information from the sensor.

[0057] When using the dyeing device of the present invention, the dyeing device needs to be erected. At this time, the third through hole 160 is usually located at the highest point of the receiving space 12.

[0058] In some specific embodiments, please refer to Figure 1 and Figure 5 The first shell 10 and the second shell 20 can cover each other and have a gap 50 .

[0059] The notch 50 is provided for exhaust. When the dyeing device of the present invention is used, the notch 50 is generally upward. On the one hand, the notch 50 is for exhaust, so as to facilitate the filling of dye; on the other hand, when the power is turned on, small bubbles will be generated in the dye liquid, which facilitates the small bubbles to gather and dissipate at the notch 50, thereby reducing the interference of the small bubbles on the uniform electric field between the electrodes.

[0060] In some specific embodiments, a hinge hole is further provided on the first shell 10, and the hinge hole is used to hinge and fix the dyeing device.

[0061] The hinge hole 170 on the first housing 10 is used to fix to some equipment or wall. When using the dyeing device, the dyeing device can be rotated to put in the biological material. Then the first housing 10 and the second housing 20 are closed, and then the dyeing device is rotated to make the first electrode plate 30 parallel to the gravity direction. Finally, the dye is poured in and the power is turned on to wait for dyeing.

[0062] In some specific embodiments, the first shell 10 and / or the second shell 20 is provided with a support column.

[0063] In this embodiment, the support column exists mainly to make the first electrode plate 30 parallel to the direction of gravity when the first shell 10 and / or the second shell 20 completely abuts against the wall or equipment.

[0064] In some specific embodiments, a mounting portion 60 is provided on the first housing 10 or the second housing 20, and the mounting portion 60 is generally hinged to a fixed object. The hinge hole 170 is provided on the mounting portion 60. When dyeing is required, the dyeing device of the utility model only needs to be rotated around the mounting portion 60 so that the notch 50 faces upward.

[0065] Finally, it should be noted that in all embodiments of the present invention, the concepts of the first shell 10 and the second shell 20 are imaginary. For example, the liquid inlet 140 and the liquid outlet 240 can be provided on one of the first shell 10 and the second shell 20 at the same time. This does not mean that the liquid inlet 140 and the liquid outlet 240 must be provided on two separate shells.

[0066] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0067] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A dyeing device, characterized in that: It includes a first shell, a second shell, a first electrode plate and a second electrode plate; the first electrode plate is arranged on the first shell, the second electrode plate is arranged on the second shell, the first shell and the second shell can cover each other and form a receiving space between the first electrode plate and the second electrode plate; the first shell is provided with a first through hole, the first through hole is connected to the first electrode; the second shell is provided with a second through hole, the second through hole is connected to the second electrode.

2. The dyeing device according to claim 1, characterized in that: The first shell is provided with a positioning hole, and the second shell is provided with a positioning protrusion, and the positioning protrusion can be inserted into the positioning hole.

3. The dyeing device according to claim 1, characterized in that: The first shell and the second shell are hinged.

4. The dyeing device according to claim 1, characterized in that: The first shell includes a first supporting member, and the second shell includes a second supporting member, the first supporting member is used to fix the first electrode plate, and the second supporting member is used to fix the second electrode plate; when the first shell and the second shell cover each other, the first supporting member and the second supporting member abut against each other, and the first electrode plate, the second electrode plate, the first supporting member and the second supporting member enclose the accommodation space.

5. The dyeing device according to claim 1, characterized in that: The first shell is provided with a liquid inlet hole, and the liquid inlet hole is connected to the receiving space to facilitate the injection of dye.

6. The dyeing device according to claim 1, characterized in that: The second shell is provided with a liquid outlet hole, and the liquid outlet hole is connected to the receiving space to facilitate the discharge of the dye.

7. The dyeing device according to claim 1, characterized in that: An annular groove is provided at the covering portion corresponding to the second shell, and an annular protrusion is provided at the second shell. When the first shell and the second shell cover each other, the annular protrusion is inserted into the annular groove.

8. The dyeing device according to claim 1, characterized in that: The second shell is further provided with a third through hole, and the third through hole is connected to the receiving space.

9. The dyeing device according to claim 1, characterized in that: The first shell and the second shell can cover each other and have a gap.

10. The dyeing device according to claim 1, characterized in that: The first shell is also provided with a mounting portion, and the mounting portion is used for hingedly fixing the dyeing device.

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