A particle capture teaching demonstration system based on vortex beam

Abstract

The utility model relates to the field of optical microscopic imaging, and provides a particle capture teaching demonstration system based on a vortex beam, comprising a vortex light field generation module, comprising a laser, and a first silver film reflector, a first plano-convex lens, a second plano-convex lens, a second silver film reflector, a linear polarizer, a quarter wave plate and a vortex wave plate arranged in sequence along an optical path, wherein the light beam generates a vortex beam through the vortex wave plate; a particle capture and manipulation module, comprising a third plano-convex lens, a fourth plano-convex lens, a third silver film reflector, a dichroic mirror, a fourth silver film reflector, a microscope objective lens and a glass slide arranged in sequence along the optical path; the third plano-convex lens is located behind the vortex wave plate; the vortex light beam reaches the glass slide through the microscope objective lens, and the light capture force thereof is used to freely manipulate multiple particles of a sample to be observed on the glass slide; an observation module, comprising a CCD camera imaging module, a white light source, a fifth plano-convex lens and a manual three-axis displacement stage.

Description

Technical Field

[0001] The utility model relates to the field of optical microscopic imaging, in particular to a particle capture teaching demonstration system based on vortex light beams. Background Art

[0002] The statements in this section merely provide background technical information related to the present invention and do not necessarily constitute prior art.

[0003] Currently, most existing particle capture systems based on vortex beams are built on professional optical platforms. They have defects such as large system size, numerous components, high experimental environment requirements, and low integration. In addition, the debugging of the optical path is usually very complicated. When students use them, they will encounter problems such as low success rate and long debugging time, making it difficult to apply them to teaching demonstrations. Utility Model Content

[0004] In order to solve the technical problems existing in the above-mentioned background technology, the utility model provides a particle capture teaching demonstration system based on vortex beam. The utility model combines vortex beam technology with particle capture technology in the same teaching demonstration system, realizes a particle capture teaching demonstration system based on vortex beam, integrates components into the same system, reduces the adjustment freedom, has the advantages of good stability, simple structure, obvious experimental phenomena, etc., so that students can preliminarily explore vortex beam and particle capture technology.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] The first aspect of the utility model provides a particle capture teaching demonstration system based on vortex beam.

[0007] A particle capture teaching demonstration system based on vortex beam, comprising:

[0008] A vortex light field generation module includes a laser, and a first silver film reflector, a first plano-convex lens, a second plano-convex lens, a second silver film reflector, a linear polarizer, a quarter wave plate, and a vortex wave plate arranged in sequence along the light path, and the light beam generates a vortex light beam through the vortex wave plate;

[0009] The particle capture and manipulation module comprises a third plano-convex lens, a fourth plano-convex lens, a third silver film reflector, a dichroic mirror, a fourth silver film reflector, a microscope objective lens and a glass slide arranged in sequence along the optical path; the third plano-convex lens is located behind the vortex wave plate; the vortex light beam passes through the microscope objective lens and reaches the glass slide, and utilizes its light capture force to freely manipulate multiple particles of the sample to be observed on the glass slide, so that the particle group moves or rotates along a specific circular trajectory;

[0010] The observation module includes a CCD camera imaging module, a white light source, a fifth plano-convex lens and a manual three-axis translation stage. The glass slide is placed on the manual three-axis translation stage. The light emitted by the white light source is focused by the fifth plano-convex lens and then irradiated onto the sample to be observed. The illumination light carrying the laser capture sample information passes through the microscope objective lens, is reflected by the fourth silver film reflector, passes through the dichroic mirror, and then is incident on the CCD camera imaging module to form an image.

[0011] Furthermore, the CCD camera imaging module includes an achromatic lens, a filter and a CCD camera, and the filter is arranged between the achromatic lens and the CCD camera.

[0012] Furthermore, the CCD camera is connected to a host computer.

[0013] Furthermore, the vortex light field generating module also includes a laser power supply connected to the laser.

[0014] Furthermore, the first plano-convex lens and the second plano-convex lens form a beam expansion system for expanding the diameter of the light beam.

[0015] Furthermore, the linear polarizer is used to convert the passing light beam into a linearly polarized light beam.

[0016] Furthermore, the quarter wave plate is used to convert a linearly polarized light beam into a circularly polarized light beam.

[0017] Furthermore, the vortex wave plate is used to convert the incident fundamental mode Gaussian beam into a vortex beam with a central hole-shaped intensity distribution.

[0018] Furthermore, the third plano-convex lens and the fourth plano-convex lens constitute a beam reduction system for reducing the diameter of the light beam.

[0019] Furthermore, the achromatic lens is used to adjust the field of view.

[0020] Compared with the prior art, the beneficial effects of the utility model are:

[0021] The utility model combines the vortex beam technology with the particle capture technology in the same teaching demonstration system, realizes a particle capture teaching demonstration system based on a vortex beam, integrates the components into the same system, reduces the degree of freedom of adjustment, has the advantages of good stability, simple structure, obvious experimental phenomena, etc., and enables students to preliminarily explore the vortex beam and particle capture technology.

[0022] The experimental operation of the utility model is simpler than the operation of the system built in a professional laboratory, and it is easier for college students who have a certain physical knowledge foundation but weak experimental ability to understand the vortex optical tweezers.

[0023] The utility model integrates the optical path while ensuring the experimental effect. Compared with other related systems, the utility model fixes the optical components, reduces the optical path adjustment required by students when using, greatly improves the experimental efficiency, and enables more students to participate in the experiment. The system designed by the utility model is small in size and has low requirements for the experimental environment, and an ordinary laboratory can meet the requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention.

[0025] Figure 1 It is a top view of a particle capture teaching demonstration system based on a vortex beam shown in the utility model;

[0026] Figure 2 It is a side view of a particle capture teaching demonstration system based on a vortex beam shown in the utility model;

[0027] Figure 3 It is a light path diagram of a particle capture teaching demonstration system based on a vortex beam shown in the utility model;

[0028] Among them, 1. laser power supply, 2. 532nm solid laser, 3. first silver film reflector, 4. first plano-convex lens, 5. second plano-convex lens, 6. second silver film reflector, 7. linear polarizer, 8. quarter wave plate, 9. vortex wave plate, 10. third plano-convex lens, 11. fourth plano-convex lens, 12. third silver film reflector, 13. dichroic mirror, 14. achromatic lens, 15. filter, 16. CCD camera, 17. fourth silver film reflector, 18. microscope objective, 19. slide, 20. fifth plano-convex lens, 21. white light source, 22. manual three-axis translation stage. DETAILED DESCRIPTION

[0029] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0030] It should be noted that the following detailed descriptions are all exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0031] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the utility model. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0032] Embodiment 1

[0033] like Figure 1 , Figure 2 , Figure 3 As shown, this embodiment provides a particle capture teaching demonstration system based on a vortex beam, including: a vortex light field generation module, a particle capture and manipulation module, and an observation module.

[0034] The vortex light field generating module comprises a laser power supply 1, a 532nm solid laser 2, a first silver film reflector 3, a first plano-convex lens 4, a second plano-convex lens 5, a second silver film reflector 6, a linear polarizer 7, a quarter wave plate 8 and a vortex wave plate 9.

[0035] In some embodiments, the diameter of the light spot emitted by the 532 nm solid-state laser 2 is about 2 mm, and the first plano-convex lens 4 and the second plano-convex lens 5 constitute a beam expansion system to expand the light spot diameter to 8 mm.

[0036] In some embodiments, the linear polarizer 7 can convert the light passing through into linear polarized light, and then after passing through a quarter wave plate 8 at a specific angle, the linear polarized light will become circularly polarized light, and finally after passing through a vortex wave plate 9, the outgoing light beam is a vortex light beam with a specific topological number.

[0037] In some embodiments, the vortex wave plate 9 can convert the incident fundamental mode Gaussian beam into a central hole type intensity distribution. For vortex wave plates 9 of different orders m, the larger the m value, the larger the size of the central hole of the outgoing beam. The vortex wave plate 9 used in the utility model has an order m of 16 and a large m value, so the size of the central hole of the outgoing beam is large, and the spot diameter passing through it needs to be enlarged to about 8 mm to produce a better vortex beam.

[0038] The particle capture and manipulation module includes a third plano-convex lens 10, a fourth plano-convex lens 11, a third silver film reflector 12, a dichroic mirror 13, a fourth silver film reflector 17, a microscope objective 18 and a glass slide 19. The third plano-convex lens 10 and the fourth plano-convex lens 11 constitute a beam reduction system, which reduces the light spot to 1 mm. After the reduced light spot enters the microscope objective 18, a stronger force of the tweezer tip can be generated.

[0039] In some embodiments, the dichroic mirror 13 can reflect light of 532 nm and transmit light of other wavelengths. The vortex light beam passes through the dichroic mirror 13 and is reflected into the microscope objective lens 18 .

[0040] In some embodiments, the glass slide 19 has a prepared sample of micronized silica particles. After the vortex light beam passes through the microscope objective 18 and reaches the glass slide 19, its light capturing force can be used to freely manipulate multiple particles, so that the particle group moves or rotates along a specific circular trajectory.

[0041] The observation module includes a manual three-axis translation stage 22, a white light source 21, a fifth plano-convex lens 20, a microscope objective 18, a fourth silver film reflector 17, a dichroic mirror 13, an achromatic lens 14, a filter 15, and a CCD camera 16. The light emitted by the white light source 21 is focused by the fifth plano-convex lens 20 and then irradiated onto the sample to be observed. The illumination light carrying the laser capture sample information passes through the microscope objective 18, is reflected by the fourth silver film reflector 17, passes through the dichroic mirror 13, and then is incident on the CCD camera 16 for imaging. The CCD camera 16 is connected to the host computer to observe the sample pool.

[0042] In some embodiments, the achromatic lens 14 can adjust the field of view and increase the brightness of light incident on the CCD camera 16 .

[0043] In some embodiments, the dichroic mirror 13 cannot completely isolate the laser, and part of the laser will pass through the dichroic mirror 13 and enter the CCD camera 16 to affect the imaging quality. Therefore, it is necessary to add a filter in front of the CCD camera 16 to further filter out the laser beam.

[0044] In some embodiments, the glass slide 19 is placed on a manual three-axis translation stage 22 , and the position of the glass slide 19 is adjusted or a new glass slide 19 is replaced by adjusting the position of the manual three-axis translation stage 22 .

[0045] The utility model uses a beam of circularly polarized light to generate vortex light after passing through a vortex wave plate 9. The light capture force of the vortex light can freely manipulate multiple particles, so that the particle group moves or rotates along a specific circular trajectory. Using a CCD camera 16 to connect a computer can display the movement of particles in real time, thereby meeting the needs of experimental teaching.

[0046] The particle capture technology adopted by the utility model is a type of technology that uses light to manipulate microparticles (such as cells). The initial optical manipulation was achieved by using the gradient force of the fundamental mode Gaussian beam. Later, some new structured light fields were also used in optical capture operations, such as the vortex beam of the Laguerre Gaussian beam. The vortex beam is a beam with a gradual phase gradient. It has a spiral phase wavefront and carries orbital angular momentum, thereby generating a phase gradient force, so that the particles can be rotated. It is of great significance for capturing tiny particles, measuring tiny forces, and producing tiny devices, and can be used in biomedicine, micro-nano processing and other fields. In addition, the system experimental effect described in the utility model is obvious, and the experimental phenomenon is interesting. Using it as a teaching demonstration system in teaching can mobilize students' enthusiasm for learning and better understand optical tweezers and vortex light technology.

[0047] The specific usage method of this embodiment is as follows:

[0048] Step 1: Connect the power cord and turn on the laser;

[0049] Step 2: Turn on the host computer, connect the CCD camera with a data cable and open the software;

[0050] Step 3: Conduct experimental operations;

[0051] Step 4: Observe the experimental phenomena.

[0052] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A particle capture teaching demonstration system based on vortex beam, characterized in that: include: A vortex light field generation module includes a laser, and a first silver film reflector, a first plano-convex lens, a second plano-convex lens, a second silver film reflector, a linear polarizer, a quarter wave plate, and a vortex wave plate arranged in sequence along the light path, and the light beam generates a vortex light beam through the vortex wave plate; The particle capture and manipulation module comprises a third plano-convex lens, a fourth plano-convex lens, a third silver film reflector, a dichroic mirror, a fourth silver film reflector, a microscope objective lens and a glass slide arranged in sequence along the optical path; the third plano-convex lens is located behind the vortex wave plate; the vortex light beam passes through the microscope objective lens and reaches the glass slide, and utilizes its light capture force to freely manipulate multiple particles of the sample to be observed on the glass slide, so that the particle group moves or rotates along a specific circular trajectory; The observation module includes a CCD camera imaging module, a white light source, a fifth plano-convex lens and a manual three-axis translation stage. The glass slide is placed on the manual three-axis translation stage. The light emitted by the white light source is focused by the fifth plano-convex lens and then irradiated onto the sample to be observed. The illumination light carrying the laser capture sample information passes through the microscope objective lens, is reflected by the fourth silver film reflector, passes through the dichroic mirror, and then is incident on the CCD camera imaging module to form an image.

2. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The CCD camera imaging module comprises an achromatic lens, a filter and a CCD camera, wherein the filter is arranged between the achromatic lens and the CCD camera.

3. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The CCD camera is connected to the host computer.

4. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The vortex light field generating module also includes a laser power supply connected to the laser.

5. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The first plano-convex lens and the second plano-convex lens form a beam expansion system for expanding the diameter of the light beam.

6. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The linear polarizer is used to convert the passing light beam into a linear polarized light beam.

7. The particle capture teaching demonstration system based on vortex beam according to claim 6 is characterized in that: The quarter wave plate is used to convert a linearly polarized light beam into a circularly polarized light beam.

8. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The vortex wave plate is used to convert the incident fundamental mode Gaussian beam into a vortex beam with a central hole-shaped intensity distribution.

9. The particle capture teaching demonstration system based on vortex beam according to claim 1 is characterized in that: The third plano-convex lens and the fourth plano-convex lens form a beam reduction system for reducing the diameter of the light beam.

10. The particle capture teaching demonstration system based on vortex beam according to claim 2, characterized in that: The achromatic lens is used to adjust the field of view.

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