Inclined blade curved surface stirring paddle, stirring device and bioreactor
By designing the inclined blade curved stirring paddle, the problem of damage to materials by the existing stirring paddle at high speed is solved, a more efficient and safer stirring process is achieved, and the performance of the bioreactor is improved.
Patent Information
- Application Number
- CN202421194749.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-29
- Publication Date
- 2025-04-08
- Estimated Expiration
- 2034-05-29
AI Technical Summary
The stirring paddles of existing laboratory biosynthesis reactors can cause physical damage to the material at high speeds and cause shear damage to sensitive materials such as proteins.
A curved surface stirring paddle of inclined blade is designed, with its blades having a smooth curved surface, the upper half of the blade is bent downward and the lower half is bent upward, the blade section is a streamlined S-shaped arc surface, and multiple through holes are provided on the blades.
Through the design of blades with gentle curvature, physical damage to the material and shear force damage is reduced, stirring efficiency and material integrity are improved, bubble generation and liquid splashing problems are reduced, and thermal utilization efficiency and cell activity of the bioreactor are improved.
Smart Images
Figure CN222724061U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biological reaction equipment, in particular to an inclined blade curved surface stirring paddle, a stirring device and a bioreactor for small biological reaction in a laboratory. Background Art
[0002] In the production process of biological products such as proteins, the primary factor affecting product quality is the bioreactor, and the agitator is an important part of the reactor. The main functions of the agitator are: breaking up air bubbles, increasing dissolved oxygen, and accelerating the mixing of materials to achieve material transfer and heat transfer.
[0003] The stirring paddles of existing laboratory biosynthesis reactors generally have the following major defects: for example, in order to match the small bioreactors used in the laboratory, the blades of the laboratory stirring paddles are usually inclined fan-shaped, and the blade profile of the fan-shaped blades is generally C-shaped, and the curvature of the blades is large. When the speed is fast during the stirring process, it will cause certain physical damage to the material, and due to the influence of shear force, it will cause damage to sensitive materials such as proteins. Therefore, the above defects need to be urgently solved. Utility Model Content
[0004] The utility model provides an inclined blade curved surface stirring paddle, a stirring device and a bioreactor, which can reduce the physical damage of blades to materials, protect the integrity of materials and reduce the damage of shear force to materials.
[0005] The utility model provides an inclined-blade curved surface stirring paddle, comprising: a stirring shaft and a stirring blade, wherein the stirring blade comprises: a coupling structure which is fixedly sleeved on the outer periphery of the stirring shaft so as to fix the stirring shaft and the stirring blade, a blade seat which is arranged on the coupling structure in an upward extending direction of the stirring shaft, and a plurality of blades which are arranged in a spaced manner along the oblique curve of the outer periphery of the blade seat, wherein the blade is a curved surface with a smooth arc and a plurality of through holes are arranged on the curved surface.
[0006] Specifically, two or more of the blade seat, the shaft coupling structure and the blades are integrally formed or welded.
[0007] Specifically, the coupling structure is provided with screw holes, and the screw holes can be used for bolts to fix the stirring shaft and the stirring blades.
[0008] Specifically, the upper part of the blade is curved downward, and the lower part of the blade is curved upward.
[0009] Preferably, there are N blade sections parallel to the outer edge of the blade from the closest to the stirring shaft to the farthest from the stirring shaft, each of the blade sections is streamlined and has a smooth S-shaped arc surface, and the blade sections have an upward curvature ρ 上and the downward curvature ρ 下 , respectively denoted as ρ 上1 , 上2 ...ρ 上N and ρ 下1 , 下2 ...ρ 下N , the ρ 上1 :ρ 上N is (1~1.2): 1, the ρ 下1 :ρ 下N (0.8~1):1;
[0010] More preferably, each of the blade sections has a 上N / ρ 下N Greater than or equal to 0.8.
[0011] Specifically, the main body of the blade is rectangular, and the main body of the blade has an angle ɑ with the axis of the stirring shaft, and the angle ɑ is less than 90°. Preferably, the angle is 30°≤ɑ≤60°, and more preferably, the angle is 40°≤ɑ≤50°.
[0012] Specifically, the number of the blades is M, and M is an even number not less than 6. Preferably, M is 6 or 8.
[0013] Specifically, the total area of the plurality of through holes on the blade accounts for 10% to 30% of the area of the blade, and the intervals between adjacent through holes are equal.
[0014] Specifically, the blade has a mirror-polished surface, and the edge of the blade and the edge of the opening of each through hole have a rounded structure.
[0015] The second aspect of the utility model also provides a stirring device, comprising the inclined-blade curved-surface stirring paddle and a motor as described in the aforementioned technical solution; the stirring shaft of the inclined-blade curved-surface stirring paddle is connected to the output shaft of the motor.
[0016] The third aspect of the utility model further provides a bioreactor, comprising the inclined blade curved surface stirring impeller described in the aforementioned technical solution or the stirring device described in the aforementioned technical solution.
[0017] The beneficial effects of the utility model are as follows:
[0018] 1. The blade is a curved surface with a smooth arc, the upper part of the blade is bent downward, and the lower part of the blade is bent upward. The blade section is not C-shaped, but an S-shaped streamlined design with a gentle curvature. The blade with a gentle curvature can reduce the resistance of the fluid to the blade, reduce the physical damage of the blade to the material, and protect the integrity of the material; for certain sensitive materials, such as certain biological products, the blade with a gentle curvature can reduce the shear force on the material and reduce the damage to the biological activity of the biological product; the blade with a gentle curvature can reduce the involvement of air, thereby reducing the generation of bubbles and reducing the problem of liquid splashing; the blade with a gentle curvature can push the liquid more evenly, and the liquid is dispersed along the curved surface with a smooth arc, reducing the flow dead angle, improving the uniformity of the temperature field, preventing local overheating from inactivating certain materials such as cells, and comprehensively improving the thermal utilization efficiency and cell activity of the bioreactor, while improving the stirring efficiency, shortening the reaction time, and improving the yield and quality of the finished product.
[0019] 2. The arrangement of the through holes in the blades will form turbulence at the through holes during the stirring process, accelerate heat and mass transfer, and greatly improve the stirring effect; especially during the fermentation process, it can promote the dissolution of oxygen in the liquid and improve the fermentation effect.
[0020] 3. Due to the large number of blades, a lower speed can effectively stir the material, which not only reduces the shear force on the material, the wear on itself and the noise on the environment caused by the high speed, but also saves energy.
[0021] 4. Although there are a large number of blades, the blades are streamlined with a gentle curvature and have a mirror-polished surface. The edges of the blades and the edges of the openings of each through hole have rounded corners, which have few sanitary dead corners and are easy to clean, helping to keep the stirring device sanitary.
[0022] 5. Suitable for a variety of different mixing materials and material viscosities, with good versatility. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] In order to more clearly illustrate the technical solution of the utility model, the following briefly introduces the drawings required for use in the utility model. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of an embodiment of the stirring paddle of the utility model;
[0025] Figure 2 A schematic diagram of a stirring blade of an embodiment of a stirring blade of the utility model;
[0026] Figure 3 A top view of a stirring blade in one embodiment of a stirring blade of the utility model;
[0027] Figure 4 This is a schematic diagram of blade sections A and B in one embodiment of a stirring paddle of the utility model;
[0028] Figure 5 This is a schematic diagram of the curvature radius of the blade section in one embodiment of the stirring paddle of the utility model;
[0029] Figure 6 It is a schematic diagram of the axial inclination angle ɑ of the blades in one embodiment of the stirring paddle of the utility model.
[0030] Reference numerals in the figure: 1. stirring shaft, 2. stirring blade, 21. connecting shaft structure, 211. screw hole, 22. blade seat, 23. blade, 231. through hole, 232. fillet structure, A. blade section, B. blade section. DETAILED DESCRIPTION
[0031] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0032] like Figures 1 to 3As shown, an embodiment of an inclined-blade curved stirring paddle provided by the utility model comprises: a stirring shaft 1 and a stirring blade 2, the stirring blade 2 comprises: a coupling structure 21 which is fixedly sleeved on the outer periphery of the stirring shaft 1 so as to fix the stirring shaft 1 and the stirring blade 2, a blade seat 22 which is arranged on the coupling structure 21 along the upward extension direction of the stirring shaft 1, and a plurality of blades 23 which are arranged in an oblique curve fit at intervals along the outer periphery of the blade seat 22, the blade 23 is a curved surface with a smooth arc and a plurality of through holes 231 are arranged on the curved surface. Specifically, the blade 23 is a curved surface with a smooth curvature, which is a streamlined design with a gentle curvature. The blade 23 with a gentle curvature can reduce the resistance of the fluid to the blade 23, reduce the physical damage of the blade 23 to the material, and protect the integrity of the material; for some sensitive materials, such as some biological products, the blade 23 with a gentle curvature can reduce the shear force on the material and reduce the damage to the biological activity of the biological product; the blade 23 with a gentle curvature can reduce the involvement of air, thereby reducing the generation of bubbles and reducing the problem of liquid splashing; the blade 23 with a gentle curvature can push the liquid more evenly, and the liquid is dispersed along the curved surface with a smooth curvature, reducing the flow dead angle, improving the uniformity of the temperature field, preventing local overheating from inactivating certain materials such as cells, and comprehensively improving the thermal utilization efficiency and cell activity of the bioreactor. While improving the stirring efficiency, it can shorten the reaction time and improve the yield and quality of the finished product. The utility model provides a kind of inclined blade curved surface stirring paddle suitable for a variety of different stirring materials and material viscosities, and has good versatility.
[0033] In one embodiment, two or more of the blade seat 22, the shaft coupling structure 21 and the blade 23 are integrally formed or welded. Specifically, the blade seat 22, the shaft coupling structure 21 and the blade 23 are integrally formed or welded by five-axis machining to form a whole stirring blade 2, so that there will be no relative displacement between the three, thereby improving the reliability and stability of the inclined blade curved stirring paddle provided by the utility model.
[0034] In one embodiment, the shaft coupling structure 21 is provided with a screw hole 211, and the screw hole 211 can be used for bolts to fix the stirring shaft 1 and the stirring blade 2. Specifically, the combination of the screw hole 211 and the bolt can improve the stability of the installation between the stirring shaft 1 and the stirring blade 2, and improve the installation efficiency.
[0035] In one embodiment, the upper part of the blade 23 is bent downward, and the lower part of the blade is bent upward. Specifically, when the inclined blade curved surface stirring paddle provided by the utility model is used, the material can realize radial flow upward or downward along the upper and lower curved parts of the blade 23, thereby forming a horizontal flow area between the inner wall of the bioreactor tank and the blade 23, and forming an obvious circulation between the upper and lower parts of the stirring blade 23, thereby improving the degree of mixing between the materials during the radial movement of the materials.
[0036] In the same embodiment, there are N blade sections parallel to the outer edge of the blade 23 from the closest to the stirring shaft 1 to the farthest from the stirring shaft 1. Each blade section is streamlined and has a smooth S-shaped arc surface. The blade section has an upward curvature ρ 上 and the downward curvature ρ 下 , respectively denoted as ρ 上1 , 上2 ...ρ 上N and ρ 下1 , 下2 ...ρ 下N , ρ 上1 :ρ 上N is (1~1.2):1,ρ 下1 :ρ 下N is (0.8~1): 1. In the same embodiment, ρ in each blade section 上N / ρ 下N Greater than or equal to 0.8.
[0037] Specifically, in fluid mechanics, the curvature of the blade 23 refers to the degree of curvature of the blade surface at different blade sections. For propellers, fans, wind turbines and other equipment, the curvature of the blade 23 has a significant impact on its performance. Figures 4-5 As shown, two blade sections are taken on the blade 23, namely blade section A and blade section B. Blade section A is taken at a position close to the stirring shaft 1, and blade section B is taken at a position farther from the stirring shaft 1. Blade section A and blade section B are both streamlined designs with smooth S-shaped arc surfaces and gentle curvature. In one embodiment, as Figure 5 As shown, the radius of curvature R1 of the upward curved surface of the blade section A is 8.89 mm, and the curvature ρ of the circle with a radius of 8.89 mm is 上1 is the reciprocal of 0.1125 mm (the reciprocal of mm is a unit); the radius of curvature R2 of the downward curved surface of the blade section A is 8.89 mm, then the curvature ρ of the circle with a radius of 8.89 mm is 下1 is the reciprocal of 0.1125 mm; the radius of curvature R3 of the curved surface of the blade section B is 9.78 mm, then the curvature ρ of the circle with a radius of 9.78 mm 上2 is the reciprocal of 0.1022 mm; the radius of curvature R4 of the curved surface of the blade section B is 7.98 mm, then the curvature ρ of the circle with a radius of 7.98 mm 下2 It is the reciprocal of 0.1271 mm. The curvatures of the overall blade profile A and blade profile B change smoothly.
[0038] In one embodiment, the main body of the blade 23 is rectangular, and the main body of the blade 23 and the axis of the stirring shaft 1 have an angle ɑ, which is less than 90°. In another embodiment, the angle is 30°≤ɑ≤60°. In another embodiment, the angle is 40°≤ɑ≤50°. Figure 6 As shown, in one embodiment, the axial tilt angle ɑ between the blade 23 and the stirring shaft 1 is 48.8°, and this angle ɑ can adapt to most working conditions, that is, this configuration can take into account both high efficiency and energy saving.
[0039] In one embodiment, the number of blades 23 is M, and M is an even number not less than 6. Preferably, M is 6 or 8. Specifically, the number of blades of the stirring paddles currently used in laboratories is relatively small, resulting in the need for a higher rotation speed for uniform stirring. The higher rotation speed not only consumes more energy, but also causes greater damage to the protein. In this embodiment, due to the larger number of blades, the material can be effectively stirred at a lower rotation speed, which not only reduces the shear force effect on the material, the wear effect on the material itself, and the noise effect on the environment caused by the high rotation speed, but also saves energy consumption.
[0040] In one embodiment, the total area of the multiple through holes 231 on the blade 23 accounts for 10% to 30% of the blade area, and the spacing between each adjacent through hole 231 is equal. Specifically, in order to match the small bioreactor used in the laboratory, the blade of the stirring paddle is usually very small. Due to the limitation of its manufacturing process or manufacturing cost, the blade is generally not provided with a through hole. The arrangement of the through holes 231 on the blade in this embodiment will form turbulence at the through hole during the stirring process, accelerate heat transfer and mass transfer, and greatly improve the stirring effect; especially in the fermentation process, it can promote the dissolution of oxygen in the liquid and improve the fermentation effect. The arrangement mode, aperture size and number of the through holes 231 can be determined according to actual needs, especially to be adapted to the size of the bioreactor. The total area of the multiple through holes 231 on the blade 23 accounts for 10% to 30% of the blade area, and the spacing between each adjacent through hole 231 is equal, which is a preferred design of the utility model.
[0041] In one embodiment, the blade 23 has a mirror-polished surface, and the edge of the blade 23 and the edge of the opening of each through hole 231 have a rounded structure. Specifically, the smooth surface can reduce the shear force of the blade 23 on the living cells in the material mixture, reduce the physical damage to the cells and other biological fine particles during the stirring process, and help maintain the biological activity of the mixture after stirring. The rounded corners can further reduce the shear force of the blade 23 on the living cells in the mixture. In addition, although the number of blades is large, because the blade is a streamlined design with a gentle curvature, the blade 23 has a mirror-polished surface, and the edge of the blade 23 and the edge of the opening of each through hole 231 have a rounded structure, so there are fewer sanitary dead corners, it is easy to clean, and it helps to keep the stirring device hygienic.
[0042] The second aspect of the utility model also provides a stirring device, including the inclined-blade curved surface stirring paddle and a motor of the aforementioned technical solution; the stirring shaft of the inclined-blade curved surface stirring paddle is connected to the output shaft of the motor, and the output shaft of the motor can drive the stirring shaft to move, thereby making the inclined-blade curved surface stirring paddle work, further realizing the technical solution and effect of the above inclined-blade curved surface stirring paddle, and the specific details are described in the above embodiments, which will not be repeated here.
[0043] The third aspect of the utility model also provides a bioreactor, including the inclined blade curved surface stirring paddle of the aforementioned technical solution or the stirring device of the aforementioned technical solution, which also has its advantages, which will not be repeated here. In one embodiment, the inclined blade curved surface stirring paddle or the stirring device containing the inclined blade curved surface stirring paddle provided by the utility model is installed in a small laboratory-level bioreactor, the tank opening of the bioreactor is relatively large, the stirring shaft 1 is fixed to the opening of the tank, and the stirring blade 2 is fixedly sleeved on the outer periphery of the stirring shaft 1. Finally, the opening of the bioreactor is fixedly combined with the tank, and the bioreactor is installed.
[0044] In one embodiment of the utility model, the stirring paddle of the utility model is used as the main component of the stirring device, and the stirring device is used as the core component of the bioreactor. Finally, the bioreactor is used for the production of 5L-level cell-free protein synthesis (referring to the tank that can accommodate 5L of biological reaction liquid), and the OD value (the yield index of living cells (yeast in this test example)) and RFU value (the mass of living cells (yeast in this test example)) of the stirred biological reaction liquid are measured. The OD value and RFU of the bioreactor using the existing inclined blade fan type stirring paddle (the blade has no through hole) for a considerable scale of cell-free protein synthesis are significantly improved, wherein the OD value is increased by more than 12%, and the RFU value is increased by more than 4%. It should be noted that in the field of biosynthesis, the OD value is proportional to the yield of living cells (yeast in this test example). The higher the OD value, the higher the yeast yield in the reaction tank. Yeast is the main raw material for cell-free protein synthesis, and yeast cell extract can provide key substances such as ribosomes for cell-free protein synthesis. The RFU value is a measure of yeast quality, commonly known as the efficacy value in the field of biosynthesis. The higher the RFU value, the better the yeast quality. It can be seen from these two test parameters that compared with the existing bioreactor with a pitch-blade fan-type stirring impeller (blades without through holes), the yeast yield and quality are significantly improved.
[0045] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A pitched blade curved surface stirring impeller, characterized in that: include: A stirring shaft and a stirring blade, wherein the stirring blade comprises: a coupling structure which is fixedly sleeved on the outer periphery of the stirring shaft so as to fix the stirring shaft and the stirring blade, a blade seat which is arranged on the coupling structure in an upward extending direction of the stirring shaft, and a plurality of blades which are arranged in an oblique curve and spaced apart along the outer periphery of the blade seat, wherein the blade is a curved surface with a smooth arc and a plurality of through holes are arranged on the curved surface.
2. The pitched blade curved surface impeller according to claim 1, characterized in that: Two or more of the blade seat, the shaft connection structure and the blades are integrally formed or welded.
3. The pitched-blade curved-surface impeller according to claim 1, characterized in that: The coupling structure is provided with a screw hole, and the screw hole can be used for bolts to fix the stirring shaft and the stirring blade.
4. The pitched-blade curved-surface impeller according to claim 1, characterized in that: The upper part of the blade is bent downward, and the lower part of the blade is bent upward.
5. The pitched-blade curved-surface impeller according to claim 4, characterized in that: There are N blade sections parallel to the outer edge of the blade from the closest to the stirring shaft to the farthest from the stirring shaft, each of which is streamlined and has a smooth S-shaped arc surface, and each blade section has an upward curvature ρ 上 and the downward curvature ρ 下 , respectively denoted as ρ 上1 , 上2 ...ρ 上N and ρ 下1 , 下2 ...ρ 下N , the ρ 上1 :ρ 上N is (1~1.2): 1, the ρ 下1 :ρ 下N is (0.8~1):
1.
6. The pitched-blade curved-surface impeller according to claim 5, characterized in that: Each of the blade sections has a 上N / ρ 下N Greater than or equal to 0.
8.
7. The pitched-blade curved-surface stirring impeller according to any one of claims 1 to 6, characterized in that: The main body of the blade is rectangular, and an angle ɑ is formed between the main body of the blade and the axis of the stirring shaft, and the angle ɑ is less than 90°.
8. The pitched-blade curved-surface impeller according to claim 7, characterized in that: The angle is 30°≤ɑ≤60°.
9. The pitched-blade curved-surface impeller according to claim 8, characterized in that: The angle is 40°≤ɑ≤50°.
10. The pitched-blade curved-surface impeller according to any one of claims 1 to 6, characterized in that: The number of the blades is M, and M is an even number not less than 6.
11. The pitched-blade curved-surface impeller according to claim 10, characterized in that: The M is 6 or 8.
12. The pitched-blade curved-surface impeller according to any one of claims 1 to 6, characterized in that: The total area of the plurality of through holes on the blade accounts for 10% to 30% of the area of the blade, and the spacings between adjacent through holes are equal.
13. The pitched-blade curved-surface impeller according to any one of claims 1 to 6, characterized in that: The blade has a mirror-polished surface, and the edge of the blade and the edge of the opening of each through hole have a rounded structure.
14. A stirring device, characterized in that: It comprises the inclined-blade curved-surface stirring paddle according to any one of claims 1 to 6 and a motor; the stirring shaft of the inclined-blade curved-surface stirring paddle is connected to the output shaft of the motor.
15. A bioreactor, characterized in that: It comprises the inclined blade curved surface stirring impeller as described in any one of claims 1 to 6 or the stirring device as described in claim 14.