Bioreactors for bacterial cultivation
By introducing a mixing unit and cleaning device into the bioreactor for bacterial culture, the problem of uneven light absorption caused by phototaxis during the culture process is solved, uniform mixing of bacterial solutions and cleaning of the inner wall of the reactor, and the growth rate and culture efficiency of bacteria are improved.
Patent Information
- Application Number
- CN202421256149.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-03
- Publication Date
- 2025-04-08
- Estimated Expiration
- 2034-06-03
AI Technical Summary
During the bacterial culture process, photosynthetic bacteria are easily concentrated and attached to the light source or light-receiving surface of the bioreactor, forming a dense photobiofilm, hindering the further transmission of light, resulting in uneven absorption of bacteria and affecting the culture effect.
A bioreactor for bacterial culture is designed, including a mixing unit and a cleaning device. The mixing unit realizes sufficient mixing of the bacterial solution through a stirring device, and the cleaning device cleans the inner wall of the reactor through the cooperation of gears and cleaning brushes to avoid bacterial accumulation.
Through the use of uniform mixing and cleaning devices, the uniform absorption of bacterial solution is ensured, the growth rate and culture efficiency of bacteria are improved, the obstruction of light transmission is avoided, and the efficiency of bacteria absorbing light sources is enhanced.
Smart Images
Figure CN222729804U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of bacterial culture, in particular to a bioreactor used for bacterial culture. Background Art
[0002] Bacterial cultivation is a vital link in the fields of biotechnology, medical research, environmental protection, etc. In order to effectively cultivate bacteria, specially designed bioreactors are required to provide a suitable growth environment and necessary culture conditions.
[0003] The existing equipment has the following disadvantages: in the traditional bacterial culture process, and because photosynthetic bacteria are phototactic, during the cultivation of photosynthetic bacteria, photosynthetic bacteria are easily concentrated and attached to the light source or light-receiving surface of the reactor, forming a dense photobiofilm, which hinders the further transmission of light and causes uneven light absorption by bacteria inside the reactor, thereby affecting the bacterial culture.
[0004] Therefore, the present application now proposes a bioreactor for bacterial culture to solve the above-mentioned problems. Utility Model Content
[0005] The utility model aims to provide a bioreactor for bacterial culture, so as to solve the above-mentioned problem of hindering further transmission of light and causing uneven light absorption by bacteria inside the reactor.
[0006] To achieve the above object, the utility model provides the following technical solution: a bioreactor for bacterial culture, comprising a reactor, a fixing frame arranged on one side of the reactor for supporting the reactor, and a lighting unit arranged on the fixing frame for providing a light source, and further comprising:
[0007] A mixing unit, disposed on the reactor, for mixing the bacterial solution inside the reactor;
[0008] The mixing unit comprises:
[0009] A stirring device, disposed inside the reactor, for stirring the solution inside the reactor;
[0010] The cleaning device is arranged on the stirring device and located inside the reactor, and is used for cleaning the inside of the reactor.
[0011] The reactor is made of a transparent acrylic plate and a cover plate is fixed on the top. A liquid outlet pipe is arranged at the lower end of the reactor, and a liquid adding pipe for adding nutrient solution to the interior of the reactor is arranged inside the liquid outlet pipe.
[0012] Among them, the stirring device includes a motor fixed on the upper end of the cover plate, a rotating shaft arranged at the power output end of the motor and passing through the cover plate, and a stirring blade fixed on the rotating shaft, the stirring blades are distributed at intervals inside the reactor, and a supporting device for improving the installation stability of the cover plate and the reactor is provided at the lower end of the cover plate, and the supporting device is sleeved on the outside of the stirring blade.
[0013] Wherein, the supporting device comprises supporting rings which are arranged at intervals inside the reactor and whose top ends are fixed on the cover plate, and a supporting frame which is fixed between the supporting rings, and the supporting frame is arranged at equal angles on the outside of the stirring blade.
[0014] Wherein, the cleaning device includes a fixed rod fixedly mounted on the rotating shaft at a position corresponding to the support ring, a connecting shaft rotatably arranged on the fixed rod on a side close to the support ring, a gear fixedly mounted on the connecting shaft and meshingly connected to the support ring, and a cleaning brush fixed on the connecting shaft between the two support rings for cleaning the inner wall of the reactor, and a tooth groove is provided on the support ring at a position corresponding to the gear.
[0015] Wherein, a scraping groove for scraping impurities on the cleaning brush is provided at the position of the support frame corresponding to the cleaning brush.
[0016] Wherein, the illumination unit comprises a light shielding plate arranged in an arc shape and fixed on the fixing frame, and an LED lamp fixed on a side of the light shielding plate close to the reactor.
[0017] Wherein, a placement frame for supporting the reactor is arranged on the fixing frame, and a fixing ring for fixing the reactor on the fixing frame is arranged at the position of the reactor corresponding to the placement frame.
[0018] Compared with the prior art, the beneficial effects of the utility model are:
[0019] The utility model is provided with a temperature regulating system and a gas regulating system on the outer side of the reactor, which are used for regulating the temperature inside the reactor and automatically regulating the oxygen and carbon dioxide concentrations inside the reactor to maintain the most suitable growth environment; the illumination intensity and illumination period inside the reactor can be adjusted through the illumination unit; during the bacterial solution cultivation process inside the reactor, the stirring device is started to stir and mix the solution inside the reactor, which can ensure that the bacterial solution inside the reactor is fully and evenly mixed, which not only improves the contact efficiency between the bacteria and the nutrient solution, but also helps the bacteria to absorb nutrients more evenly, thereby promoting the growth rate of the bacteria and improving the cultivation efficiency; and during the rotation of the mixing device, the cleaning device will be driven to rotate, and the cleaning device cleans the inside of the reactor, which can prevent phototactic bacteria from accumulating on the inner wall of the light-receiving surface of the reactor, hindering the transmission of light and affecting the efficiency of photosynthetic bacteria in absorbing the light source, thereby improving the growth reaction efficiency of the photosynthetic bacteria. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the main structure in one embodiment of the utility model;
[0021] Figure 2 This is a schematic diagram of a cross-section structure of an embodiment of the utility model;
[0022] Figure 3 This is a schematic diagram of the structure of a top view of the interior of a reactor in one embodiment of the utility model;
[0023] Figure 4 This is a schematic diagram of the structure inside the reactor in one embodiment of the utility model;
[0024] Figure 5 This is a schematic diagram of the explosion structure in one embodiment of the utility model;
[0025] Figure 6 This is a structural schematic diagram of a fixing frame in one embodiment of the utility model;
[0026] Figure 7 This is a schematic diagram of the structure of a mixing unit in one embodiment of the utility model;
[0027] Figure 8 This is a schematic diagram of the structure of the connection between the cleaning device and the support ring in one embodiment of the utility model;
[0028] Fig. 9 It is a schematic structural diagram of a cleaning device in one embodiment of the utility model.
[0029] In the figure: 1. Reactor; 11. Cover plate; 12. Liquid outlet pipe; 13. Liquid adding pipe; 14. Fixed ring; 2. Mixing unit; 21. Motor; 22. Rotating shaft; 23. Stirring blade; 24. Support ring; 241. Tooth groove; 25. Support frame; 251. Scraping groove; 26. Cleaning device; 261. Fixed rod; 262. Connecting shaft; 263. Gear; 264. Cleaning brush; 3. Fixed frame; 31. Placement frame; 4. Illumination unit; 41. Sunshade; 42. LED lamp. DETAILED DESCRIPTION
[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0031] See also Figure 1-9 The utility model provides a technical solution: a bioreactor for bacterial culture, comprising a reactor 1, a fixing frame 3 arranged on one side of the reactor 1 for supporting the reactor 1, and a lighting unit 4 arranged on the fixing frame 3 for providing a light source, and also comprising:
[0032] A mixing unit 2 is provided on the reactor 1 and is used to mix the bacterial solution inside the reactor 1;
[0033] The mixing unit 2 comprises:
[0034] A stirring device, disposed inside the reactor 1, for stirring the solution inside the reactor 1;
[0035] The cleaning device 26 is arranged on the stirring device and located inside the reactor 1 , and is used for cleaning the inside of the reactor 1 .
[0036] It should be noted that, when working, a temperature regulating system and a gas regulating system are installed on the outside of the reactor 1, which are used to adjust the temperature inside the reactor 1 and automatically adjust the oxygen and carbon dioxide concentrations inside the reactor 1 to maintain the most suitable growth environment. The illumination unit 4 can adjust the illumination intensity and illumination period inside the reactor 1. During the cultivation of the bacterial solution inside the reactor 1, the stirring device is started to stir and mix the solution inside the reactor 1, which can ensure that the bacterial solution inside the reactor 1 is fully and evenly mixed, which not only improves the contact efficiency between the bacteria and the nutrient solution, but also helps the bacteria to absorb nutrients more evenly, thereby promoting the growth rate of bacteria and improving the cultivation efficiency. In addition, during the rotation of the mixing device, the cleaning device 26 will be driven to rotate. The cleaning device 26 cleans the inside of the reactor 1, which can prevent phototactic bacteria from accumulating on the inner wall of the light-receiving surface of the reactor 1, hindering the transmission of light and affecting the efficiency of photosynthetic bacteria absorbing light sources, thereby improving the growth reaction efficiency of photosynthetic bacteria.
[0037] In one embodiment, the reactor 1 is made of a transparent acrylic plate and a cover plate 11 is fixed on the top. A liquid outlet pipe 12 is provided at the lower end of the reactor 1. A liquid adding pipe 13 for adding nutrient solution to the reactor 1 is provided inside the liquid outlet pipe 12.
[0038] With this design, the reactor 1 has high light transmittance, which can improve the absorption efficiency of photosynthetic bacteria to light sources. The cover plate 11 is removed from the upper end of the reactor 1, and then the bacteria that need to react are added to the interior of the reactor 1. The liquid outlet pipe 12 can discharge the cultivated bacteria from the interior of the reactor 1, and the nutrient solution can be added to the interior of the reactor 1 through the liquid adding pipe 13.
[0039] In one embodiment, the stirring device includes a motor 21 fixed to the upper end of the cover plate 11, a rotating shaft 22 arranged at the power output end of the motor 21 and passing through the cover plate 11, and a stirring blade 23 fixed on the rotating shaft 22, the stirring blade 23 is distributed at intervals inside the reactor 1, and a supporting device for improving the installation stability of the cover plate 11 and the reactor 1 is provided at the lower end of the cover plate 11, and the supporting device is sleeved on the outer side of the stirring blade 23.
[0040] With this design, when the motor 21 is started, the rotating shaft 22 drives the stirring blades 23 to rotate, so that the stirring blades 23 fully mix the bacteria and nutrient solution inside the reactor 1. The supporting device abuts against the inside of the reactor 1, which can improve the stability of the stirring device installed in the reactor 1, thereby further improving the mixing efficiency of the stirring device for the bacterial solution.
[0041] In one embodiment, the support device includes support rings 24 spaced apart inside the reactor 1 and fixed at their top ends on the cover plate 11 , and support frames 25 fixed between the support rings 24 , and the support frames 25 are arranged at equal angles on the outside of the stirring blades 23 .
[0042] With this design, a plurality of support frames 25 are provided, which are in contact with the interior of the reactor 1, and the stability of the installation of the entire mixing device can be improved. The support ring 24 is connected to the cleaning device 26, and can drive the cleaning device 26 to rotate during the rotation of the rotating shaft 22. There is no need to set up a power source, which can improve the utilization rate of energy.
[0043] In one embodiment, the cleaning device 26 includes a fixed rod 261 fixedly mounted on the rotating shaft 22 at a position corresponding to the support ring 24, a connecting shaft 262 rotatably arranged on the side of the fixed rod 261 close to the support ring 24, a gear 263 fixedly mounted on the connecting shaft 262 and meshingly connected to the support ring 24, and a cleaning brush 264 fixed on the connecting shaft 262 and located between the two support rings 24 for cleaning the inner wall of the reactor 1, and a tooth groove 241 is provided at the position of the support ring 24 corresponding to the gear 263.
[0044] With such a design, during the rotation of the rotating shaft 22, the gear 263 is meshed with the support ring 24, so that the gear 263 drives the connecting shaft 262 to rotate, and then the connecting shaft 262 drives the cleaning brush 264 to clean the inside of the reactor 1, so that the inner wall of the reactor 1 remains clean, and the reactor 1 can maintain high light transmittance, thereby improving the absorption efficiency of photosynthetic bacteria to light sources.
[0045] In one embodiment, a scraping groove 251 for scraping impurities on the cleaning brush 264 is formed at the position of the support frame 25 corresponding to the cleaning brush 264 .
[0046] With such a design, the cleaning brush 264 passes through the scraping groove 251 opened on the support frame 25 when passing through the support frame 25. The scraping groove 251 can scrape the impurities on the cleaning brush 264, improve the cleanliness of the cleaning brush 264, and further improve the cleaning efficiency of the cleaning brush 264 on the inner wall of the reactor 1. When the bacteria in the reactor 1 are not phototactic bacteria, the cleaning brush 264 can effectively remove the residues and bacterial metabolites on the inner wall of the reactor 1, maintain the cleanliness of the culture environment, and provide a continuous good growth environment for the bacteria. When the bacterial culture in the reactor 1 is completed, the cover plate 11 is removed from the reactor 1, and the entire mixing unit 2 is taken out from the inside of the reactor 1 for cleaning.
[0047] In one embodiment, the illumination unit 4 includes an arc-shaped shading plate 41 fixed on the fixing frame 3 and an LED lamp 42 fixed on a side of the shading plate 41 close to the reactor 1 .
[0048] This design uses an LED lamp 42 with high energy efficiency and long life, adjustable light intensity and light cycle, and a semi-circular arc-shaped shading plate 41 covering the outside of the reactor 1, which can concentrate the light on the inside of the reactor 1 and improve the absorption efficiency of the bacteria inside the reactor 1 to the light source.
[0049] In one embodiment, a placement frame 31 for supporting the reactor 1 is disposed on the fixing frame 3 , and a fixing ring 14 for fixing the reactor 1 on the fixing frame 3 is disposed at a position of the reactor 1 corresponding to the placement frame 31 .
[0050] With such a design, when installing the reactor 1 , the fixing ring 14 on the reactor 1 is inserted into the placement frame 31 on the fixing frame 3 , so that the installation and fixing of the reactor 1 can be completed quickly.
Claims
1. A bioreactor for bacterial culture, comprising a reactor (1), a fixing frame (3) arranged on one side of the reactor (1) for supporting the reactor (1), and a lighting unit (4) arranged on the fixing frame (3) for providing a light source, characterized in that: Also includes: A mixing unit (2), arranged on the reactor (1), and used for mixing the bacterial solution inside the reactor (1); The mixing unit (2) comprises: a stirring device, arranged inside the reactor (1) and used for stirring the solution inside the reactor (1); a cleaning device (26), arranged on the stirring device and located inside the reactor (1), and used for cleaning the inside of the reactor (1); The reactor (1) is made of a transparent acrylic plate and has a cover plate (11) fixed on the top. A liquid outlet pipe (12) is provided at the lower end of the reactor (1). A liquid adding pipe (13) for adding nutrient solution to the interior of the reactor (1) is provided inside the liquid outlet pipe (12). The stirring device comprises a motor (21) fixed to the upper end of the cover plate (11), a rotating shaft (22) arranged at the power output end of the motor (21) and passing through the cover plate (11), and a stirring blade (23) fixed to the rotating shaft (22), wherein the stirring blades (23) are distributed at intervals inside the reactor (1), and a supporting device for improving the installation stability of the cover plate (11) and the reactor (1) is arranged at the lower end of the cover plate (11), and the supporting device is sleeved on the outer side of the stirring blade (23); The supporting device comprises supporting rings (24) arranged at intervals inside the reactor (1) and having their top ends fixed on the cover plate (11), and supporting frames (25) fixedly arranged between the supporting rings (24), wherein the supporting frames (25) are arranged at equal angles on the outside of the stirring blades (23); The cleaning device (26) comprises a fixed rod (261) fixedly mounted on the rotating shaft (22) at a position corresponding to the support ring (24), a connecting shaft (262) rotatably mounted on the fixed rod (261) on a side close to the support ring (24), a gear (263) fixedly mounted on the connecting shaft (262) and meshingly connected to the support ring (24), and a cleaning brush (264) fixed on the connecting shaft (262) between the two support rings (24) for cleaning the inner wall of the reactor (1), wherein the support ring (24) is provided with a tooth groove (241) at a position corresponding to the gear (263).
2. The bioreactor for bacterial culture according to claim 1, characterized in that: A scraping groove (251) for scraping impurities on the cleaning brush (264) is provided at a position of the support frame (25) corresponding to the cleaning brush (264).
3. The bioreactor for bacterial culture according to claim 1, characterized in that: The illumination unit (4) comprises a light shielding plate (41) arranged in an arc shape and fixed on the fixing frame (3), and an LED lamp (42) fixed on a side of the light shielding plate (41) close to the reactor (1).
4. The bioreactor for bacterial culture according to claim 1, characterized in that: The fixing frame (3) is provided with a placement frame (31) for supporting the reactor (1), and a fixing ring (14) for fixing the reactor (1) on the fixing frame (3) is provided at a position of the reactor (1) corresponding to the placement frame (31).