Animal cell culture bioreactor tank

Abstract

The utility model discloses a bioreactor tank body for animal cell culture, which relates to the technical field of animal cell culture, and comprises a base, a bracket fixedly installed above the base, a fixing ring fixedly installed above the bracket, a reaction tank sleeved in the fixing ring, a sliding rod fixedly installed above the base, two sliding rods are provided, and the two sliding rods are symmetrically arranged, a top plate fixedly installed on the top of the sliding rod, a driving component sleeved on the sliding rod, a magnet fixedly installed on the output end of the driving component, the magnet fits with the reaction tank, a sealing cover detachably installed above the reaction tank, an interactive valve fixedly installed above the sealing cover, two interactive valves are symmetrically provided, a drain pipe fixedly installed at the bottom of the reaction tank, a drain valve fixedly installed on the outside of the drain pipe, and a stirring component fixedly installed in the reaction tank. The utility model has more stable driving, more uniform stirring, and reduced damage to cells.

Description

Technical Field

[0001] The utility model belongs to the field of animal cell culture, and in particular relates to a bioreactor tank for animal cell culture. Background Art

[0002] In the process of animal cell culture, mechanical stirring is often used to stir the animal cells and their culture fluid in the animal cell culture bioreactor, so that the animal cells and their culture fluid have good mixing and concentration uniformity, which is a better culture effect of the animal cells, thereby meeting the needs of actual experiments. However, when the existing animal cell culture bioreactor tank is used for cell culture, the stirring method often affects the survival rate and culture effect of the animal cell culture.

[0003] The Chinese patent with the publication number CN210945623U discloses a bioreactor tank for animal cell culture, and relates to the field of animal cell culture technology. The utility model includes a base, a fixed ring, a plurality of collars, a tank cover and a set of handles. A plurality of support plates are fixed to one surface of the base, a tank body is fixed to one surface of the plurality of support plates, a pad is fixed to one surface of the base, an electromagnet is fixed to one surface of the pad, and a cycle timing switch is fixed to the other surface of the pad. The utility model controls the energization of the electromagnet gap through the cycle timing switch, so that the electromagnet is intermittently magnetized, so that the electromagnet is periodically magnetic. During the magnetization process, the electromagnet will drive the fixed ring to rise along the inner wall of the tank. When the electromagnet is not magnetic, the fixed ring will descend along the inner wall of the tank under the action of its own gravity, so that the plurality of collars on the fixed ring drive the fan blades thereon to stir the animal cell culture solution inside the tank, thereby making cell culture safer and more efficient.

[0004] However, the above technical solution uses an electromagnet to push the fixed ring up. As the fixed ring rises, the distance between the electromagnet and the fixed ring increases, the magnetism becomes weaker and weaker, and the fixed ring is subject to a greater force of gravity, which makes the rising space of the fixed ring limited and unstable. At the same time, the water resistance on the surface of the fan blade is large, which makes it difficult to rotate on the one hand, and causes damage to the cells in the culture medium on the other hand. Utility Model Content

[0005] The utility model provides an animal cell culture bioreactor tank body, which is used to solve the technical problems raised by the above-mentioned background technology.

[0006] In order to solve the above technical problems, the utility model provides an animal cell culture bioreactor tank body, including a base, a bracket fixedly installed above the base, a fixing ring fixedly installed above the bracket, a reaction tank sleeved in the fixing ring, a sliding rod fixedly installed above the base, two sliding rods are provided, and the two sliding rods are symmetrically arranged, a top plate is fixedly installed on the top of the sliding rod, a driving assembly is sleeved on the sliding rod, a magnet is fixedly installed on the output end of the driving assembly, and the magnet is fitted with the reaction tank, a sealing cover is detachably installed above the reaction tank, an interactive valve is fixedly installed above the sealing cover, and two interactive valves are symmetrically provided, a drain pipe is fixedly installed at the bottom of the reaction tank, a drain valve is fixedly installed on the outside of the drain pipe, and a stirring assembly is fixedly installed in the reaction tank.

[0007] Preferably, the bracket is provided in plurality and the plurality of brackets are arranged in an array, and the fixing ring is fixedly connected to the outer side of the reaction tank.

[0008] Preferably, the driving assembly includes a push plate, through holes are provided on both sides of the push plate, the sliding rod passes through the through holes, a fixing plate is fixedly installed above the sealing cover, a threaded rod is provided between the fixing plate and the top plate, two ends of the threaded rod are rotatably connected to the fixing plate and the top plate respectively, and a threaded hole is provided in the middle of the push plate, the threaded rod passes through the threaded hole, and the threaded rod is meshed with the threaded hole, a motor is fixedly installed above the top plate, the output end of the motor is fixedly connected to the threaded rod, a sleeve is provided on the sliding rod, the top of the sleeve is fixedly connected to the push plate, and the magnet is fixedly installed on the sleeve.

[0009] Preferably, the stirring assembly includes a slide rail, the slide rail is fixedly installed on the inner wall of the reaction tank, two slide rails are provided, the two slide rails are symmetrically arranged, a magnetic slider is slidably installed in the slide rail, the magnetic slider is adsorbed and connected to a magnet, and a stirring element is fixedly installed between the two magnetic sliders.

[0010] Preferably, the stirring member comprises a central axis, the central axis is fixedly installed between the two magnetic sliders, a sleeve 2 is sleeved on the outer side of the central axis, two sleeves 2 are symmetrically provided, and agitator plates are fixedly installed on the outer sides of the two sleeves 2.

[0011] Preferably, the agitator plates are provided in plurality and arranged in an array, the agitator plates are provided with filter holes, the filter holes are provided in plurality and are evenly arranged on the surface of the agitator plates.

[0012] Compared with the prior art, the utility model has the following advantages:

[0013] 1. The utility model provides an animal cell culture bioreactor tank body, which is provided with a driving component. The driving component can drive the magnet to slide up and down along the outer wall of the reaction tank, thereby driving the magnetic slider of the stirring component to slide in the slide rail, thereby driving the central axis to move up and down, and mixing and stirring the culture solution through the stirring plate, so that the driving is more stable and the stirring is more uniform.

[0014] 2. The utility model provides an animal cell culture bioreactor tank, wherein filter holes are provided above the agitator plate, and the filter holes are evenly arranged. When the agitator plate rotates, the culture fluid can pass through the filter holes, and the cells can also interact through the filter holes, thereby reducing damage to the cells. Moreover, the arrangement of the filter holes reduces the water resistance of the agitator plate, thereby improving the rotation efficiency of the second sleeve. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a structural diagram of a bioreactor tank for animal cell culture according to the utility model;

[0016] Figure 2 This is a structural diagram of a driving component in a tank of an animal cell culture bioreactor of the utility model;

[0017] Figure 3 This is a diagram of the internal structure of a reaction tank in a bioreactor tank for animal cell culture according to the utility model;

[0018] Figure 4 This is a structural diagram of a stirring assembly in a tank of an animal cell culture bioreactor of the utility model;

[0019] Numbers in the figure: 1. base; 2. bracket; 3. fixing ring; 4. sliding rod; 5. top plate; 6. driving assembly; 7. interactive valve; 8. reaction tank; 9. drain pipe; 10. drain valve; 11. sealing cover; 12. magnet; 13. sleeve one; 14. push plate; 15. motor; 16. threaded rod; 17. fixing plate; 18. stirring assembly; 19. slide rail; 20. magnetic slider; 21. agitator plate; 22. sleeve two; 23. center axis; 24. filter hole. DETAILED DESCRIPTION

[0020] See also Figure 1-4The utility model provides a technical solution: a bioreactor tank for animal cell culture, comprising a base 1, a bracket 2 fixedly installed above the base 1, a fixing ring 3 fixedly installed above the bracket 2, a reaction tank 8 sleeved in the fixing ring 3, a sliding rod 4 fixedly installed above the base 1, two sliding rods 4 are provided, and the two sliding rods 4 are symmetrically arranged, a top plate 5 fixedly installed on the top of the sliding rod 4, a driving component 6 sleeved on the sliding rod 4, a magnet 12 fixedly installed on the output end of the driving component 6, the magnet 12 is fitted with the reaction tank 8, a sealing cover 11 is detachably installed above the reaction tank 8, an interactive valve 7 is fixedly installed above the sealing cover 11, and two interactive valves 7 are symmetrically provided, a drain pipe 9 is fixedly installed at the bottom of the reaction tank 8, a drain valve 10 is fixedly installed on the outside of the drain pipe 9, and a stirring component 18 is fixedly installed in the reaction tank 8.

[0021] In the present embodiment, by setting a driving component 6, the driving component 6 can drive the magnet 12 to slide up and down along the outer wall of the reaction tank 8, thereby driving the magnetic slider 20 of the stirring component 18 to slide in the slide rail 19, thereby driving the central axis 23 to move up and down, and the culture medium is mixed and stirred by the agitator 21, the driving is more stable, and the stirring is more uniform. Filter holes 24 are opened above the agitator 21, and the filter holes 24 are evenly arranged. When the agitator 21 rotates, the culture medium can pass through the filter holes 24, and the cells can also interact through the filter holes 24, reducing damage to the cells. The arrangement of the filter holes 24 makes the water resistance of the agitator 21 lower, thereby improving the rotation efficiency of the sleeve 22.

[0022] Furthermore, a plurality of brackets 2 are provided, and the plurality of brackets 2 are arranged in an array, and the fixing ring 3 is fixedly connected to the outer side of the reaction tank 8 .

[0023] Furthermore, the driving assembly 6 includes a push plate 14, through holes are opened on both sides of the push plate 14, the sliding rod 4 passes through the through holes, a fixing plate 17 is fixedly installed above the sealing cover 11, a threaded rod 16 is provided between the fixing plate 17 and the top plate 5, both ends of the threaded rod 16 are rotatably connected to the fixing plate 17 and the top plate 5 respectively, and a threaded hole is opened in the middle of the push plate 14, the threaded rod 16 passes through the threaded hole, and the threaded rod 16 is engaged with the threaded hole, a motor 15 is fixedly installed above the top plate 5, and the output end of the motor 15 is fixedly connected to the threaded rod 16, a sleeve 13 is sleeved on the sliding rod 4, the top of the sleeve 13 is fixedly connected to the push plate 14, and the magnet 12 is fixedly installed on the sleeve 13.

[0024] Furthermore, the stirring assembly 18 includes a slide rail 19, and the slide rail 19 is fixedly installed on the inner wall of the reaction tank 8. There are two slide rails 19, and the two slide rails 19 are symmetrically arranged. A magnetic slider 20 is slidably installed in the slide rail 19, and the magnetic slider 20 is adsorbed and connected to the magnet 12. A stirring element is fixedly installed between the two magnetic sliders 20.

[0025] Furthermore, the stirring member includes a central shaft 23 , the central shaft 23 is fixedly installed between the two magnetic sliders 20 , a sleeve 22 is sleeved outside the central shaft 23 , two sleeves 22 are symmetrically provided, and agitator plates 21 are fixedly installed outside the two sleeves 22 .

[0026] Furthermore, a plurality of the agitator plates 21 are provided, and the plurality of the agitator plates 21 are arranged in an array. A filter hole 24 is opened on the agitator plate 21 , and a plurality of the filter holes 24 are provided, and the plurality of filter holes 24 are evenly arranged on the surface of the agitator plate 21 .

[0027] Working principle:

[0028] When in use, firstly, the culture fluid and cell fluid are put into the reaction tank 8 through the interactive valve 7, and the motor 15 circuit is started. The motor 15 rotates to drive the threaded rod 16 to rotate, and the push plate 14 is engaged with the threaded rod 16, so that as the forward and reverse rotation of the motor 15 is changed, the push plate 14 can be driven to slide up and down along the slide rod 4, and the push plate 14 drives the sleeve 13 to slide up and down along the slide rod 4, thereby pushing the magnet 12 to slide along the outside of the reaction tank 8, thereby driving the magnetic slider 20 to slide in the slide rail 19, and the magnetic slider 20 drives the central axis 23 to move up and down in the reaction tank 8, so that the drive is more stable and the stirring is more uniform. A filter hole 24 is opened above the stirring plate 21, and the filter holes 24 are evenly arranged. When the stirring plate 21 rotates, the culture fluid can pass through the filter hole 24, and the cells can also interact through the filter hole 24, thereby reducing damage to the cells. In addition, the arrangement of the filter hole 24 makes the water resistance of the stirring plate 21 lower, thereby improving the rotation efficiency of the sleeve 22.

Claims

1. An animal cell culture bioreactor tank, comprising a base (1), characterized in that: A bracket (2) is fixedly mounted on the top of the base (1), a fixing ring (3) is fixedly mounted on the top of the bracket (2), a reaction tank (8) is sleeved inside the fixing ring (3), a sliding rod (4) is fixedly mounted on the top of the base (1), two sliding rods (4) are provided, and the two sliding rods (4) are symmetrically arranged, a top plate (5) is fixedly mounted on the top of the sliding rod (4), a driving component (6) is sleeved on the sliding rod (4), a magnet (12) is fixedly mounted on the output end of the driving component (6), and the magnet (12) is fitted with the reaction tank (8), a sealing cover (11) is detachably mounted on the top of the reaction tank (8), an interactive valve (7) is fixedly mounted on the top of the sealing cover (11), and two interactive valves (7) are symmetrically provided, a drain pipe (9) is fixedly mounted on the bottom of the reaction tank (8), a drain valve (10) is fixedly mounted on the outside of the drain pipe (9), and a stirring component (18) is fixedly mounted inside the reaction tank (8).

2. The animal cell culture bioreactor tank according to claim 1, characterized in that: The brackets (2) are provided in plurality, and the plurality of brackets (2) are arranged in an array, and the fixing ring (3) is fixedly connected to the outside of the reaction tank (8).

3. The animal cell culture bioreactor tank according to claim 1, characterized in that: The driving assembly (6) comprises a push plate (14), through holes are provided on both sides of the push plate (14), the slide rod (4) passes through the through holes, a fixed plate (17) is fixedly installed above the sealing cover (11), a threaded rod (16) is provided between the fixed plate (17) and the top plate (5), two ends of the threaded rod (16) are rotatably connected to the fixed plate (17) and the top plate (5), a threaded hole is provided in the middle of the push plate (14), the threaded rod (16) passes through the threaded hole, and the threaded rod (16) is meshed with the threaded hole, a motor (15) is fixedly installed above the top plate (5), the output end of the motor (15) is fixedly connected to the threaded rod (16), a sleeve (13) is sleeved on the slide rod (4), the top of the sleeve (13) is fixedly connected to the push plate (14), and the magnet (12) is fixedly installed on the sleeve (13).

4. The animal cell culture bioreactor tank according to claim 1, characterized in that: The stirring assembly (18) comprises a slide rail (19), the slide rail (19) is fixedly mounted on the inner wall of the reaction tank (8), two slide rails (19) are provided, and the two slide rails (19) are symmetrically arranged. A magnetic slider (20) is slidably mounted in the slide rail (19), the magnetic slider (20) is adsorbed and connected to the magnet (12), and a stirring element is fixedly mounted between the two magnetic sliders (20).

5. The animal cell culture bioreactor tank according to claim 4, characterized in that: The stirring member comprises a central shaft (23), the central shaft (23) is fixedly installed between the two magnetic sliders (20), a sleeve 2 (22) is sleeved on the outside of the central shaft (23), two sleeves 2 (22) are symmetrically provided, and agitator plates (21) are fixedly installed on the outside of the two sleeves 2 (22).

6. The animal cell culture bioreactor tank according to claim 5, characterized in that: The agitator plates (21) are provided in plurality and arranged in an array. The agitator plates (21) are provided with filter holes (24), and the filter holes (24) are provided in plurality and are evenly arranged on the surface of the agitator plates (21).

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