L-serine fermentation liquor separation and purification system

Abstract

The utility model relates to the technical field of L-serine, in particular to an L-serine fermentation liquor separation and purification system. Comprising an L-serine fermentation liquor storage tank, a first filtering device, a second filtering device, first concentration equipment, an extraction kettle, a back extraction kettle, second concentration equipment, a first crystallization kettle, an L-serine crude product storage tank, a decoloration kettle, a second crystallization kettle, a drying device and an L-serine product storage tank. The separation and purification system is reasonable in design, and the yield, the yield and the purity of the L-serine product are greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of L-serine, in particular to a separation and purification system for L-serine fermentation liquid. Background Art

[0002] L-serine is a non-essential amino acid with important physiological functions and is widely used in the fields of medicine, cosmetics, and chemical industry. At present, the main methods for producing L-serine are chemical synthesis, protein hydrolysis, enzymatic conversion, and microbial fermentation. Among them, microbial fermentation is a method for producing amino acids that has emerged with the development of modern biotechnology. The key to microbial fermentation is to select amino acid production strains with great potential industrial value through mutagenesis breeding and gene editing, and then combine related engineering fermentation technology to achieve large-scale and efficient production of amino acids.

[0003] At present, fermentation is the most commonly used method for producing L-serine, but the fermentation broth contains a large amount of sugars, proteins, amino acids, organic acids, metal ions and other compounds that affect the purification of L-serine, directly affecting the output, yield and purity of L-serine products. Therefore, in view of the above problems, it is necessary to develop a separation and purification system for L-serine fermentation broth. Utility Model Content

[0004] The technical problem to be solved by the utility model is: in view of the deficiencies of the prior art, a separation and purification system for L-serine fermentation liquid is provided, and the production, yield and purity of the L-serine product can be improved by utilizing the separation and purification system.

[0005] In order to solve the above technical problems, the technical solution of the utility model is:

[0006] A system for separating and purifying an L-serine fermentation liquid comprises an L-serine fermentation liquid storage tank, wherein a discharge port of the L-serine fermentation liquid storage tank is connected to a first filtering device, a filtrate outlet of the first filtering device is connected to a second filtering device, a filtrate outlet of the second filtering device is connected to a first concentrating device, a concentrated liquid outlet of the first concentrating device is connected to an extraction kettle, an organic phase outlet of the extraction kettle is connected to a stripping kettle, an aqueous phase outlet of the stripping kettle is connected to a second concentrating device, a concentrated liquid outlet of the second concentrating device is connected to a first crystallization kettle, a crystal outlet of the first crystallization kettle is connected to an L-serine crude product storage tank, a discharge port of the L-serine crude product storage tank is connected to a decolorization kettle, a decolorized liquid outlet of the decolorization kettle is connected to a second crystallization kettle, a crystal outlet of the second crystallization kettle is connected to a drying device, and a discharge port of the drying device is connected to an L-serine product storage tank.

[0007] As an improved technical solution, the first filtering device is a ceramic membrane filtering device with a pore size range of 50-200nm.

[0008] As an improved technical solution, the second filtering device is an ultrafiltration membrane filtering device with a molecular weight cutoff of 5000-10000Da.

[0009] As an improved technical solution, the first concentration device is a nanofiltration membrane concentration device with a molecular weight cutoff of 100-200Da.

[0010] As an improved technical solution, the extraction kettle includes a kettle body, a feed port and an extractant inlet are provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and trapezoidal stirring plates are provided at the upper and lower parts of the stirring shaft respectively, and a plurality of stirring rods are provided on the trapezoidal stirring plates.

[0011] As an improved technical solution, the stripping kettle includes a kettle body, the top of the kettle body is provided with a feed inlet and a stripping agent inlet, the bottom of the kettle body is provided with a discharge port, the inside of the kettle body is provided with a stirring shaft, one end of the stirring shaft is connected to a motor, the upper and lower parts of the stirring shaft are respectively provided with square stirring frames, and the square stirring frames are provided with multiple stirring rods.

[0012] As an improved technical solution, the first crystallization kettle and the second crystallization kettle both include a kettle body, a feed port is provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a liquid outlet is provided on one side of the lower part of the kettle body, a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft.

[0013] After adopting the above technical solution, the beneficial effects of the utility model are:

[0014] The L-serine fermentation liquid separation and purification system comprises an L-serine fermentation liquid storage tank, a discharge port of the L-serine fermentation liquid storage tank is connected to a first filter device, a filtrate outlet of the first filter device is connected to a second filter device, a filtrate outlet of the second filter device is connected to a first concentrating device, a concentrated liquid outlet of the first concentrating device is connected to an extraction kettle, an organic phase outlet of the extraction kettle is connected to a stripping kettle, an aqueous phase outlet of the stripping kettle is connected to a second concentrating device, a concentrated liquid outlet of the second concentrating device is connected to a first crystallization kettle, a crystal outlet of the first crystallization kettle is connected to an L-serine crude product storage tank, a discharge port of the L-serine crude product storage tank is connected to a decolorization kettle, a decolorized liquid outlet of the decolorization kettle is connected to a second crystallization kettle, a crystal outlet of the second crystallization kettle is connected to a drying device, and a discharge port of the drying device is connected to an L-serine product storage tank. In actual production, the fermentation liquid in the L-serine fermentation liquid storage tank enters the interior of the first filter device along the pipeline under the action of the delivery pump, the filtered filtrate enters the second filter device along the pipeline under the action of the delivery pump for further filtration, the collected filtrate enters the interior of the first concentration device along the pipeline under the action of the delivery pump, the concentrated liquid collected after concentration enters the extraction kettle, the organic phase after extraction by adding the extractant enters the stripping kettle along the pipeline, the stripping agent is added, the water phase collected after stripping enters the second concentration device, the concentrated liquid enters the first crystallization kettle along the pipeline under the action of the delivery pump, after cooling and crystallizing, the collected crystal liquid enters the L-serine crude product storage tank, and then the liquid after adding water, heating, stirring and dissolving enters the decolorization kettle under the action of the delivery pump, after decolorization treatment by the decolorant, the decolorized liquid collected by suction filtration enters the second crystallization kettle, the crystals collected after cooling and crystallizing are dried by the drying device, and the obtained product is stored in the L-serine product storage tank. The above separation and purification system is reasonably designed and greatly improves the output, yield and purity of the L-serine product.

[0015] Since the first filtering device is a ceramic membrane filtering device with a pore size range of 50-200nm, bacteria and proteins can be effectively removed.

[0016] Since the second filtering device is an ultrafiltration membrane filtering device with a molecular weight cutoff of 5000-10000Da, the protein can be further effectively removed.

[0017] Since the first concentration device is a nanofiltration membrane concentration device with a molecular weight cutoff of 100-200Da, effective concentration of L-serine can be achieved.

[0018] Since the extraction kettle includes a kettle body, a feed port and an extractant inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a trapezoidal stirring plate is provided on the upper and lower parts of the stirring shaft respectively, and a plurality of stirring rods are provided on the trapezoidal stirring plate. In actual production, the feed liquid and the extractant enter the interior of the kettle body from the feed port and the extractant inlet respectively, and after the motor is started, the stirring shaft, the trapezoidal stirring plate and the plurality of stirring rods are driven to rotate, and the feed liquid and the extractant inside the kettle body are fully stirred and mixed, so that the extractant is fully in contact with the feed liquid, and after standing and stratification, the aqueous phase and the organic phase are discharged from the discharge port respectively. The extraction kettle with the above structure is reasonably designed and improves the extraction efficiency.

[0019] Since the stripping kettle includes a kettle body, a feed inlet and a stripping agent inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a square stirring frame is provided on the upper and lower parts of the stirring shaft respectively, and a plurality of stirring rods are provided on the square stirring frame. In actual production, the organic phase and the stripping agent enter the interior of the kettle body from the feed inlet and the stripping agent inlet respectively. After the motor is started, the stirring shaft, the square stirring frame and the plurality of stirring rods are driven to rotate, and the aqueous phase and the stripping agent inside the kettle body are fully stirred and mixed, so that the stripping agent is fully in contact with the aqueous phase. After standing and stratification, the aqueous phase and the organic phase are discharged from the discharge port respectively. The extraction kettle of the above structure is reasonably designed and improves the stripping efficiency.

[0020] Since both the first crystallization kettle and the second crystallization kettle include a kettle body, a feed inlet is provided on the top of the kettle body, a discharge outlet is provided on the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a liquid outlet is provided on one side of the lower part of the kettle body, a filter screen is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft. The feed liquid enters the inside of the kettle body, the refrigerant in the jacket is used to cool the feed liquid, the stirring shaft and the stirring rod are used to evenly cool the feed liquid, the crystallization mother liquor is extracted from the liquid outlet of the kettle body by a suction pump, the crystals are intercepted by the filter screen, and finally discharged from the discharge outlet. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the structure of a system for separating and purifying L-serine fermentation broth according to the utility model;

[0022] Among them, 1-L-serine fermentation liquid storage tank, 2-first filtering device, 3-second filtering device, 4-first concentration equipment, 5-extraction kettle, 6-back extraction kettle, 7-second concentration equipment, 8-first crystallization kettle, 9-L-serine crude product storage tank, 10-decolorization kettle, 11-second crystallization kettle, 12-drying device, 13-L-serine product storage tank. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0024] A system for separating and purifying L-serine fermentation broth, such as Figure 1 As shown, it includes an L-serine fermentation liquid storage tank 1, the discharge port of the L-serine fermentation liquid storage tank 1 is connected to a first filter device 2 (a ceramic membrane filter device with a pore size range of 50-200nm), the filtrate outlet of the first filter device 2 is connected to a second filter device 3 (an ultrafiltration membrane filter device with a molecular weight cutoff of 5000-10000Da), the filtrate outlet of the second filter device 3 is connected to a first concentration device 4 (a nanofiltration membrane concentration device with a molecular weight cutoff of 100-200Da), the concentrate outlet of the first concentration device 4 is connected to an extraction kettle 5, the organic phase outlet of the extraction kettle 5 is connected to a stripping kettle 6, and the aqueous phase outlet of the stripping kettle 6 is connected to a second concentration device 7 (a concentration kettle, including a kettle body, the outside of the kettle body is provided with a jacket, the top and bottom of the kettle body are respectively provided with a feed inlet and a concentrate outlet, and the kettle body A stirring shaft is provided inside, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft), the concentrated liquid outlet of the second concentrating equipment 7 is connected to the first crystallization kettle 8, the crystal outlet of the first crystallization kettle 8 is connected to the L-serine crude product storage tank 9, the discharge port of the L-serine crude product storage tank 9 is connected to the decolorization kettle 10, the decolorization kettle 10 (including a kettle body, the top of the kettle body is provided with a feed inlet and a decolorant inlet, the bottom of the kettle body is provided with a decolorant outlet, one side of the lower part of the kettle body is provided with a decolorant outlet, the inner wall of the kettle body is provided with a filter screen at a position corresponding to the decolorant outlet, and the interior of the kettle body is provided with an anchor stirrer) The decolorant outlet is connected to the second crystallization kettle 11, the crystal outlet of the second crystallization kettle 11 is connected to a drying device 12 (double cone dryer), and the discharge port of the drying device 12 is connected to the L-serine product storage tank 13.

[0025] In actual production, the fermentation liquid in the L-serine fermentation liquid storage tank enters the interior of the first filter device along the pipeline under the action of the delivery pump (which can effectively remove bacteria and protein), the filtered filtrate enters the second filter device along the pipeline under the action of the delivery pump for further filtration (which can further effectively remove protein), the collected filtrate enters the interior of the first concentration device along the pipeline under the action of the delivery pump (which can achieve effective concentration of L-serine), the concentrated liquid collected after concentration enters the extraction kettle, the organic phase after extraction by adding the extractant enters the stripping kettle along the pipeline, the stripping agent is added, the aqueous phase collected after stripping enters the second concentration device, the concentrated liquid enters the first crystallization kettle along the pipeline under the action of the delivery pump, after cooling, crystallizing and filtering, the collected crystal liquid enters the L-serine crude product storage tank, and then the liquid after adding water, heating, stirring and dissolving enters the decolorization kettle under the action of the delivery pump, after decolorization treatment by the decolorizer, the decolorized liquid collected by suction filtration enters the second crystallization kettle, the collected crystals after cooling and crystallizing are dried by a drying device, and the obtained product is stored in the L-serine product storage tank. The separation and purification system is reasonably designed and greatly improves the output, yield and purity of the L-serine product.

[0026] The extraction kettle 3 includes a kettle body, a feed inlet and an extractant inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and the upper and lower parts of the stirring shaft are respectively provided with trapezoidal stirring plates, and a plurality of stirring rods are provided on the trapezoidal stirring plates. In actual production, the feed liquid and the extractant enter the interior of the kettle body from the feed inlet and the extractant inlet respectively. After the motor is started, the stirring shaft, the trapezoidal stirring plate and the plurality of stirring rods are driven to rotate, and the feed liquid and the extractant inside the kettle body are fully stirred and mixed, so that the extractant is fully in contact with the feed liquid, and after standing and stratification, the aqueous phase and the organic phase are discharged from the discharge port respectively. The extraction kettle of the above structure is reasonably designed and improves the extraction efficiency.

[0027] The stripping kettle 6 includes a kettle body, a feed inlet and a stripping agent inlet are provided on the top of the kettle body, a discharge port is provided on the bottom of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a square stirring frame is provided on the upper and lower parts of the stirring shaft, respectively, and a plurality of stirring rods are provided on the square stirring frame. In actual production, the organic phase and the stripping agent enter the interior of the kettle body from the feed inlet and the stripping agent inlet, respectively. After the motor is started, the stirring shaft, the square stirring frame and the plurality of stirring rods are driven to rotate, and the aqueous phase and the stripping agent inside the kettle body are fully stirred and mixed, so that the stripping agent is fully in contact with the aqueous phase, and after standing and stratifying, the aqueous phase and the organic phase are discharged from the discharge port, respectively. The extraction kettle of the above structure is reasonably designed, and the stripping efficiency is improved.

[0028] The first crystallization kettle 8 and the second crystallization kettle 11 both include a kettle body, a feed inlet is provided at the top of the kettle body, a discharge outlet is provided at the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a liquid outlet is provided on one side of the lower part of the kettle body, a filter screen is provided at a position corresponding to the liquid outlet on the inner wall of the kettle body, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft. The feed liquid enters the inside of the kettle body, the refrigerant in the jacket is used to cool the feed liquid, the stirring shaft and the stirring rod are used to evenly cool the feed liquid, the crystallization mother liquor is extracted from the liquid outlet of the kettle body by a suction pump, the crystals are intercepted by the filter screen, and finally discharged from the discharge outlet.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A system for separating and purifying L-serine fermentation broth, characterized in that: The invention comprises an L-serine fermentation liquid storage tank, wherein the discharge port of the L-serine fermentation liquid storage tank is connected to a first filtering device, the filtrate outlet of the first filtering device is connected to a second filtering device, the filtrate outlet of the second filtering device is connected to a first concentrating device, the concentrated liquid outlet of the first concentrating device is connected to an extraction kettle, the organic phase outlet of the extraction kettle is connected to a stripping kettle, the aqueous phase outlet of the stripping kettle is connected to a second concentrating device, the concentrated liquid outlet of the second concentrating device is connected to a first crystallization kettle, the crystal outlet of the first crystallization kettle is connected to an L-serine crude product storage tank, the discharge port of the L-serine crude product storage tank is connected to a decolorization kettle, the decolorized liquid outlet of the decolorization kettle is connected to a second crystallization kettle, the crystal outlet of the second crystallization kettle is connected to a drying device, and the discharge port of the drying device is connected to an L-serine product storage tank.

2. A L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The first filtering device is a ceramic membrane filtering device with a pore size range of 50-200nm.

3. A L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The second filtering device is an ultrafiltration membrane filtering device with a molecular weight cutoff of 5000-10000Da.

4. A L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The first concentration device is a nanofiltration membrane concentration device with a molecular weight cutoff of 100-200Da.

5. A L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The extraction kettle comprises a kettle body, a feed port and an extractant inlet are arranged at the top of the kettle body, a discharge port is arranged at the bottom of the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected to a motor, a trapezoidal stirring plate is arranged at the upper and lower parts of the stirring shaft respectively, and a plurality of stirring rods are arranged on the trapezoidal stirring plate.

6. The L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The stripping kettle comprises a kettle body, a feed inlet and a stripping agent inlet are arranged at the top of the kettle body, a discharge port is arranged at the bottom of the kettle body, a stirring shaft is arranged inside the kettle body, one end of the stirring shaft is connected to a motor, a square stirring frame is arranged at the upper and lower parts of the stirring shaft respectively, and a plurality of stirring rods are arranged on the square stirring frame.

7. The L-serine fermentation broth separation and purification system according to claim 1, characterized in that: The first crystallization kettle and the second crystallization kettle both include a kettle body, a feed port is provided at the top of the kettle body, a discharge port is provided at the bottom of the kettle body, a jacket is provided on the outside of the kettle body, a liquid outlet is provided on one side of the lower part of the kettle body, a filter is provided on the inner wall of the kettle body at a position corresponding to the liquid outlet, a stirring shaft is provided inside the kettle body, one end of the stirring shaft is connected to a motor, and a plurality of stirring rods are provided on the stirring shaft.

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