Supporting device of disposable bioreactor

Abstract

The utility model discloses a supporting device of a disposable bioreactor, which comprises a cylinder module, a lifting motor module and a transfer base module, the cylinder module is connected to the transfer base module, the lifting motor module is mounted on the cylinder module, and disposable bag supporting equipment for culturing cells is formed by the cylinder module, the lifting motor module and the transfer base module. Compared with the prior art, the device has the advantages that the device is matched with a safe and environment-friendly disposable bioreactor, is formed by heat sealing and welding, is used in a consumable discarding manner, has no trouble of cleaning and cleaning verification, is free of sterilization, occupies a smaller space area than stainless steel equipment, is convenient to install, and is suitable for popularization and application. And the cost is lower than that of stainless steel equipment under the condition that the early-stage culture process is immature. Import is transferred to localization, so that the delivery period and the cost are reduced.

Description

Technical Field

[0001] The utility model relates to supporting equipment for a bioreactor, in particular to a supporting device for a disposable bioreactor. Background Art

[0002] In the production and manufacturing process of biological products, the use of bioreactors is indispensable. Generally, bioreactors are divided into reusable and disposable systems. Reusable bioreactors are generally divided into small and micro glass bioreactors used in product development and process optimization and stainless steel bioreactors used in pilot and large-scale production. Reusable bioreactors need to be fully sterilized before use, and the reactor system needs to be cleaned after batch culture. Especially in the production and development of food and drugs, strict cleaning verification of equipment cleaning is required, which is time-consuming and labor-intensive. This method of sterilizing the reactor before use and cleaning and verification after use not only increases a lot of labor costs, but also prolongs the entire product development cycle, so it is not necessarily cost-effective to calculate the cost as a whole.

[0003] There are many disposable bioreactors on the market, such as disposable oscillating cell bags for cell culture. Due to the limitation of their mass transfer method, their cell density is difficult to compare with that of stirred bioreactors. In addition, this structure is also difficult to achieve scale-up production. There are also bag-type disposable stirred bioreactors, which are limited by their structural pressure tolerance and ventilation mode, and their mass transfer performance is not very good, and cannot meet the cultivation needs of cells / microorganisms with high oxygen demand.

[0004] Therefore, developing a support device for a disposable bioreactor has become an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] The utility model is to solve the above-mentioned shortcomings and provide a disposable bioreactor support device. The device adopts safe and environmentally friendly disposable materials, plastic film molding, and no need for cleaning. It can be efficiently applied to production and process characterization without the trouble of cleaning and cleaning verification. It can meet the mass transfer system requirements of various cells / microorganisms.

[0006] The above-mentioned purpose of the utility model is achieved through the following technical scheme: a support device for a disposable bioreactor, including a barrel module, a lifting motor module and a transfer base module, the barrel module is connected to the transfer base module, and the lifting motor module is installed on the barrel module, together forming a disposable bag support device for culturing cells.

[0007] Furthermore, the cylinder module includes an inner cylinder, which is provided with a door assembly, the door assembly is provided with a transparent acrylic window, the lower side of the door assembly is provided with a sampling window, the lower part and the bottom of the inner cylinder are provided with a jacketed cylinder, a cavity is formed between the jacketed cylinder and the inner cylinder, the bottom of the cavity is provided with a water inlet, the upper side wall of the cavity is provided with a water outlet, the water inlet and the water outlet are welded with sanitary-grade TC flanges for the inlet and outlet of cooling water for temperature control; the bottom of the inner cylinder is provided with a bottom harvesting port that passes through the jacketed cylinder; the upper part of the inner cylinder is provided with an insulation cylinder, and the insulation layer is provided inside the insulation cylinder.

[0008] Furthermore, the barrel module is an overall cylindrical structure.

[0009] Furthermore, the outer wall of the jacketed cylinder is provided with a first hidden bracket for hiding the pipeline of the disposable bioreactor (disposable oscillating cell bag).

[0010] Furthermore, the outer wall of the jacketed cylinder is provided with a second hidden bracket for hiding the power cord of the stirring motor, which effectively reduces the clutter caused by numerous pipelines.

[0011] Furthermore, the inner bottom of the inner cylinder is designed to be funnel-shaped, which can reduce liquid residue.

[0012] Furthermore, the lifting motor module includes a lifting device and a stirring motor assembly. The lifting device is fixed to the top of the cylinder module through a left lifting support bracket and a right lifting support bracket. The stirring motor assembly is fixed to the lifting device through a left stirring connecting bridge and a right stirring connecting bridge. The lifting device manually adjusts the up and down floating position of the motor through a hand wheel to facilitate the installation of the disposable bag body.

[0013] Furthermore, a stirring connection bridge reinforcement plate is connected between the left stirring connection bridge and the right stirring connection bridge.

[0014] Furthermore, a pipeline bracket is connected to the stirring connection bridge reinforcement plate through a pipeline bracket fixing piece, and an exhaust gas filter bracket is installed on the left stirring connection bridge and the right stirring connection bridge through screws. The exhaust gas filter bracket can float up and down with the stirring motor, and can also move left and right through screws. The exhaust gas filter bracket is used to prevent the bending of the disposable bag filter pipeline from causing pressure leakage and fixing the filter position.

[0015] Furthermore, the transport base module includes a base bracket, casters and a weighing sensor, wherein the casters are fixed to the bottom of the base bracket, and the weighing sensor is fixed to the upper end surface of the base bracket. The weighing sensor is fixed to the four legs of the barrel module by welding or threading, and the data of the weighing sensor can be viewed on the disposable bioreactor control cabinet to effectively control the feed and the overall culture volume.

[0016] Compared with the prior art, the utility model has the following advantages: the utility model is equipped with a safe and environmentally friendly disposable bioreactor (disposable oscillating cell bag), heat-sealed and welded, and disposable consumables are used. The equipment does not require cleaning and cleaning verification, is sterilization-free, occupies less space than stainless steel equipment, and is easy to install. It is cheaper than stainless steel equipment when the early culture process is immature. Imports are converted to domestic production, which reduces delivery time and costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional structural schematic diagram of one side of the utility model.

[0018] Figure 2 It is a three-dimensional structural schematic diagram of the other side of the utility model.

[0019] Figure 3 It is a structural schematic diagram of one side of the door assembly of the utility model.

[0020] Figure 4 It is a schematic diagram of the exploded structure of the utility model.

[0021] Figure 5 It is a structural schematic diagram of the cylinder module in the utility model.

[0022] Figure 6 It is a schematic diagram of the internal structure of the cylinder module in the utility model.

[0023] Figure 7 It is a structural schematic diagram of one side of the lifting motor module in the utility model.

[0024] Figure 8 It is a top view structural schematic diagram of the lifting motor module in the utility model.

[0025] Fig. 9 It is a structural schematic diagram of the transfer base module in the utility model. DETAILED DESCRIPTION

[0026] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0027] like Figures 1 to 4 As shown, a support device of a disposable bioreactor includes a barrel module 1, a lifting motor module 2 and a transfer base module 3. The barrel module 1 and the lifting motor module 2 are combined together by threaded fixing, which can be easily disassembled and installed. The barrel module 1 and the transfer base module 3 are combined together by threading or welding, and it is not recommended to disassemble them when not necessary. Thus, the barrel module 1, the lifting motor module 2 and the transfer base module 3 are combined into one to form a movable culture space.

[0028] like Figure 5 , Figure 6 As shown, the cylinder module 1 includes an inner cylinder 101, a jacket cylinder 102, an insulation cylinder 103, an insulation layer 104, an insulation sealing plate 105, a door assembly 106, a transparent acrylic window 107, a sampling window 108, a sanitary welded TC flange (water inlet) 109, a sanitary welded TC flange (water outlet) 110 and a bottom harvesting port 111. The cylinder module 1 is an overall cylindrical structure. The inner cylinder 101 The inner bottom is set to be funnel-shaped to reduce liquid residue. The inner cylinder 101 is provided with a door assembly 106, and a transparent acrylic window 107 is provided on the door assembly 106. A sampling window 108 is provided on the lower side of the door assembly 106. The top of the inner cylinder 101 is welded and fixed to the jacket cylinder 102 through the insulation sealing plate 105, the jacket guide plate and the bottom harvesting port 111. The insulation cylinder 103 is arranged on the upper part of the jacket cylinder 102 and the inner cylinder 101. The jacket cylinder 102 is provided with a water inlet and a water outlet at the bottom and side, respectively, and a sanitary welding TC flange (water inlet) 109 and a sanitary welding TC flange (water outlet) are welded respectively. The jacket guide plate adopts the bottom inlet and side outlet method to achieve temperature control. The cylinder module 1 is made of S30408 ​​stainless steel.

[0029] like Figure 2 As shown, the outer wall of the jacketed cylinder 102 is also provided with a first hidden bracket 112 for hiding the pipeline of the disposable bioreactor (disposable oscillating cell bag); the outer wall of the jacketed cylinder 102 is provided with a second hidden bracket 113 for hiding the power cord of the stirring motor, which effectively reduces the clutter caused by the numerous pipelines.

[0030] like Figure 7 , Figure 8 As shown, the lifting motor module 2 includes a right lifting support bracket 201, a left lifting support bracket 202, a lifting device 203, a right stirring connection bridge 204, a left stirring connection bridge 205, a filter bracket assembly 206, a stirring motor assembly 207, a pipeline bracket 208, a pipeline bracket fixing piece 209 and a stirring connection bridge reinforcement plate 210. Among them, the stirring motor assembly 207 is fixed by threads through the right stirring connection bridge 204 and the left stirring connection bridge 205, and the lifting device 203 is fixed by threads with the right stirring connection bridge 204, the left stirring connection bridge 205 and the right lifting support bracket 201 and the left lifting support bracket 202 before being assembled to the top of the cylinder module 1. The stirring connection bridge reinforcement plate 210 strengthens the support strength of the right stirring connection bridge 204 and the left stirring connection bridge 205 to the stirring motor assembly 207. The pipeline support 208 and the pipeline support fixing part 209 are arranged on the back of the stirring connection bridge reinforcement plate 210 to hide the stirring motor power line and increase the overall appearance. The lifting motor module 2 is made of S30408 ​​stainless steel.

[0031] like Fig. 9 As shown, the transfer base module 3 includes a base bracket 301, a caster 302 and a weighing sensor 303. The caster 302 is installed and fixed at the bottom of the base bracket 301, and the weighing sensor 303 is fixed to the upper end surface of the base bracket 301. The transfer base module 3 is connected and fixed to the cylinder module 1 by welding or threading the weighing sensor 303.

[0032] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A support device for a disposable bioreactor, characterized in that: The invention comprises a cylinder module, a lifting motor module and a transfer base module, wherein the cylinder module is connected to the transfer base module, and the lifting motor module is installed on the cylinder module, and together they form a disposable bag support device for culturing cells; the cylinder module comprises an inner cylinder, and the inner cylinder is provided with a door assembly, and the door assembly is provided with a transparent acrylic window, and a sampling window is provided on the lower side of the door assembly, and a jacket cylinder is provided at the lower part and the bottom of the inner cylinder, and a cavity is formed between the jacket cylinder and the inner cylinder, and a water inlet is provided at the bottom of the cavity, and a water outlet is provided on the upper side wall of the cavity, and the water inlet and the water outlet are both welded with sanitary TC flanges for the inlet and outlet of cooling water for temperature control; the bottom of the inner cylinder is provided with a through-hole through the jacket cylinder, and a through-hole through which the through-hole passes. Harvesting port at the bottom; an insulation cylinder is provided on the upper part of the inner cylinder, and an insulation layer is provided inside the insulation cylinder; the lifting motor module includes a lifting device and a stirring motor assembly, the lifting device is fixed to the top of the cylinder module through a left lifting support bracket and a right lifting support bracket, the stirring motor assembly is fixed to the lifting device through a left stirring connecting bridge and a right stirring connecting bridge, and the lifting device manually adjusts the up and down floating position of the motor through a hand wheel; the transfer base module includes a base bracket, casters and a weighing sensor, the casters are installed and fixed to the bottom of the base bracket, the weighing sensor is fixed to the upper end face of the base bracket, and the weighing sensor is fixed to the four legs of the cylinder module by welding or threaded connection.

2. A support device for a disposable bioreactor according to claim 1, characterized in that: The barrel module is in a cylindrical structure as a whole.

3. The support device for a disposable bioreactor according to claim 1, characterized in that: The outer wall of the jacketed cylinder is provided with a first hidden bracket for hiding the bioreactor pipeline.

4. The support device for a disposable bioreactor according to claim 1, characterized in that: The outer wall of the jacket cylinder is provided with a second hidden bracket for hiding the power line of the stirring motor.

5. The support device for a disposable bioreactor according to claim 1, characterized in that: The inner bottom of the inner cylinder is designed to be funnel-shaped.

6. The support device for a disposable bioreactor according to claim 1, characterized in that: A stirring connection bridge reinforcement plate is connected between the left stirring connection bridge and the right stirring connection bridge.

7. A disposable bioreactor support device according to claim 6, characterized in that: The stirring connection bridge reinforcement plate is connected with a pipeline support through a pipeline support fixing piece, and the exhaust gas filter support is installed on the left stirring connection bridge and the right stirring connection bridge through screws.

Images