Micro-fluidic chip reagent perfusion positioning mistake-proofing tool
By designing a microfluidic chip reagent infusion positioning and error-proof tooling, the through holes on the replaceable tooling cover plate correspond to the reaction chamber, the problem of inaccurate reagent infusion in the microfluidic chip is solved, and efficient and accurate reagent infusion is achieved.
Patent Information
- Application Number
- CN202421870099.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-02
- Publication Date
- 2025-04-18
- Estimated Expiration
- 2034-08-02
AI Technical Summary
How to ensure that the corresponding reagents are accurately and accurately pre-filled in each reaction chamber of the microfluidic chip to avoid errors that are prone to direct infusion.
A microfluidic chip reagent infusion positioning and error-proof tooling is designed, including tooling base and replaceable tooling cover. The tool base is used to carry and position the microfluidic chip. The tool cover can be replaced with through holes corresponding to the reaction chamber. By replacing different tool covers, reagents can be accurately poured into each reaction chamber.
In the production of microfluidic chips, different reagents are accurately poured into the corresponding reaction chambers, avoiding errors during direct perfusion and improving the accuracy and efficiency of perfusion.
Smart Images
Figure CN222766368U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a tool for producing a microfluidic chip, in particular to a tool for preventing errors in positioning the reagent perfusion of a microfluidic chip. Background Art
[0002] Microfluidic chips can integrate a series of basic operation units involved in the fields of chemistry and biology, such as sample preparation, reaction, separation, and detection, into a small chip. At the same time, the network formed by the microchannels can run through the entire system, with the advantages of precision, high efficiency, miniaturization of instruments, low reagent consumption, and small sample usage. Microfluidic chips are usually designed with multiple flow channels to distribute the samples to be tested into multiple reaction chambers, and multiple items of testing can be performed on the same sample.
[0003] How to ensure that the corresponding reagents are accurately pre-infused into each reaction chamber is an urgent problem to be solved in this field.
[0004] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the utility model, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to ordinary technicians in this field. Utility Model Content
[0005] Purpose of the utility model: The technical problem to be solved by the utility model is to provide a microfluidic chip reagent perfusion positioning error-proof tooling in view of the deficiencies in the prior art, which can accurately perfuse different reagents into the corresponding reaction chambers of the microfluidic chip.
[0006] In order to solve the above technical problems, the utility model discloses a microfluidic chip reagent perfusion positioning error-proofing tool, which includes:
[0007] A tooling base, comprising a chip carrying portion for carrying a microfluidic chip and a chip positioning portion for positioning the microfluidic chip;
[0008] And a replaceable tooling cover plate, the replaceable tooling cover plate is provided with a through hole corresponding to the reaction chamber to be filled in the microfluidic chip; when the replaceable tooling cover plate is installed on the tooling base, the replaceable tooling cover plate covers the microfluidic chip carried on the chip carrying part, and the through hole is opposite to the reaction chamber to be filled.
[0009] The utility model covers the microfluidic chip with a replaceable tooling cover plate, and uses the through holes on the tooling cover plate to inject the corresponding reagent into the facing reaction chamber. Then, by replacing different replaceable tooling covers, the through holes on different replaceable tooling covers correspond to different reaction chambers in each detection unit, so that different reagents can be accurately injected into the corresponding reaction chambers of the microfluidic chip during the production of the microfluidic chip, avoiding the error-prone situation during direct injection.
[0010] Specifically, the chip carrying parts are provided with more than two, and are arranged in a linear array on the tooling base along the length direction of the tooling base.
[0011] Specifically, the chip carrying portion includes a chip supporting surface for supporting the microfluidic chip and a receiving space formed above the chip supporting surface for the microfluidic chip to enter and exit, and the receiving space can at least partially receive the microfluidic chip.
[0012] More specifically, the chip carrying portion is a groove structure, which is arranged on the top surface of the tooling base and recessed inwardly; the chip supporting surface is formed at the bottom of the groove structure, and the inner cavity of the groove structure corresponds to the accommodating space.
[0013] Furthermore, two opposite side walls of the groove structure are provided with two notches for clamping and removing the microfluidic chip.
[0014] Specifically, the height between the chip support surface and the top surface of the tooling base is smaller than the height of the microfluidic chip. When the replaceable tooling cover is installed on the tooling base, the replaceable tooling cover is pressed on the microfluidic chip.
[0015] Specifically, the chip positioning portion is two or more chip positioning pins protruding upwardly and arranged on the chip supporting surface; and the microfluidic chip is formed with two or more chip positioning pin holes corresponding to the two or more chip positioning pins.
[0016] Specifically, the replaceable tooling cover is detachably connected to the tooling base via a cover positioning pin.
[0017] Specifically, the top surfaces of both ends of the tooling base in the length direction are provided with the cover plate positioning pins; and the replaceable tooling cover is provided with cover plate positioning pin holes corresponding to the cover plate positioning pins.
[0018] Furthermore, the replaceable tooling cover is also provided with an avoidance through hole for avoiding the chip positioning pin.
[0019] Beneficial effects:
[0020] The utility model covers the microfluidic chip with a replaceable tooling cover plate, and uses the through holes on the tooling cover plate to inject the corresponding reagent into the facing reaction chamber. Then, by replacing different replaceable tooling covers, the through holes on different replaceable tooling covers correspond to different reaction chambers in each detection unit, so that different reagents can be accurately injected into the corresponding reaction chambers of the microfluidic chip during the production of the microfluidic chip, avoiding the error-prone situation during direct injection. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods, and the above and / or other advantages of the present invention will become more clear.
[0022] Figure 1 This is a top view of the centrifugal microfluidic chip disclosed in the first embodiment of the utility model.
[0023] Figure 2 This is an exploded view of the microfluidic chip reagent perfusion positioning error-proofing tooling disclosed in the first embodiment of the utility model after being assembled with a microfluidic chip.
[0024] Figure 3 for Figure 2 The microfluidic chip reagent perfusion positioning error-proofing tooling shown is a top view after being assembled with the microfluidic chip.
[0025] Figure 4 This is a process diagram for replacing different replaceable tooling covers disclosed in the first embodiment of the utility model.
[0026] The description of the accompanying drawings is:
[0027] 1. Tooling base; 11. Chip carrying part; 111. Chip supporting surface; 112. Accommodating space; 113. Notch; 12. Chip positioning part; 13. Top surface; 14. Cover plate positioning pin; 2. Microfluidic chip; 21. Reaction chamber; 22. Chip positioning pin hole; 23. Detection unit; 3. Replaceable tooling cover plate; 3-1. First replaceable cover plate; 3-2. Second replaceable cover plate; 31. Through hole; 31-1. First through hole; 31-2. Second through hole; 32. Cover plate positioning pin hole; 33. Avoidance through hole. DETAILED DESCRIPTION
[0028] See also Figure 1 The centrifugal microfluidic chip 2 usually has a rotation center, on which there are two or more detection units 23 arranged in a circular array around the rotation center. Each detection unit 23 is provided with two or more reaction chambers 21 for accommodating different reagents, so as to perform multiple tests on the same sample. There are more than two detection units 23 arranged in a circular array, so as to realize parallel detection of more than two samples.
[0029] See also Figure 2 and Figure 3 The utility model discloses a microfluidic chip reagent perfusion positioning error-proof tooling, which is used to accurately perfuse different reagents into the corresponding reaction chamber 21 of each detection unit 23 in the microfluidic chip 2 when the microfluidic chip 2 is produced, avoiding the situation that is easy to make mistakes during direct perfusion.
[0030] See also Figure 2 The error-proofing tooling includes a tooling base 1 and a replaceable tooling cover 3. The tooling base 1 includes a chip carrying portion 11 for carrying the microfluidic chip 2 and a chip positioning portion 12 for positioning the microfluidic chip 2. The replaceable tooling cover 3 is provided with a through hole 31 corresponding to the reaction chamber 21 to be filled in the microfluidic chip 2. When the replaceable tooling cover 3 is installed on the tooling base 1, the replaceable tooling cover 3 covers the microfluidic chip 2 carried on the chip carrying portion 11, and the through hole 31 is directly opposite to the reaction chamber 21 to be filled.
[0031] In this embodiment, by replacing different replaceable tooling covers 3, the through holes 31 on the different replaceable tooling covers 3 can correspond to different reaction chambers 21 in each detection unit 23, so that when the microfluidic chip 2 is produced, different reagents can be accurately injected into the corresponding reaction chambers 21 of the microfluidic chip 2, avoiding the error-prone situation during direct injection.
[0032] In one embodiment, see Figure 3 , the through hole 31 may be a conical hole, the diameter of which gradually decreases from top to bottom. Figure 3 The diameter of the lower opening of the conical hole is slightly larger than the top opening of the reaction chamber.
[0033] In one embodiment, see Figure 2 There are more than two chip carrying parts 11, and they are arranged in a linear array on the tooling base 1 along the length direction of the tooling base 1.
[0034] In this embodiment, by arranging more than two chip carrying parts 11 in a linear array on the tooling base 1, batch perfusion of the same reagent can be achieved, which helps to improve the production efficiency of the microfluidic chip.
[0035] In some embodiments, see Figure 2 The chip carrier 11 includes a chip supporting surface 111 for supporting the microfluidic chip 2 and a receiving space 112 formed above the chip supporting surface 111 for the microfluidic chip 2 to enter and exit. The receiving space 112 can at least partially receive the microfluidic chip 2. In some specific embodiments, the chip supporting surface 111 is a plane.
[0036] In one embodiment, see Figure 2The chip carrying portion 11 is a groove structure, which is arranged on the top surface 13 of the tooling base 1 and is recessed inward; the chip supporting surface 111 is formed at the bottom of the groove structure, and the inner cavity of the groove structure corresponds to the accommodating space 112.
[0037] It should be understood that the shape of the groove may be compatible with the microfluidic chip 2, such as a cylindrical groove, or may not be compatible.
[0038] In one embodiment, see Figure 2 In order to facilitate taking out the microfluidic chip 2 , two oppositely disposed side walls of the groove structure are provided with two notches 113 for clamping and taking out the microfluidic chip 2 .
[0039] In this embodiment, after the replaceable tooling cover plate 3 is removed, the microfluidic chip 2 can be taken out by inserting an external clamping member, such as an operator's finger, into the notch 113 to pinch and pick up the microfluidic chip 2. This method of taking out is convenient and easy to operate.
[0040] In one embodiment, the height between the chip support surface 111 and the top surface 13 of the tooling base 1 is less than the height of the microfluidic chip 2, so that when the replaceable tooling cover plate 3 is installed on the tooling base 1, the replaceable tooling cover plate 3 is pressed on the microfluidic chip 2. In another embodiment, the height between the chip support surface 111 and the top surface 13 of the tooling base 1 is slightly higher than the height of the microfluidic chip 2, so that when the replaceable tooling cover plate 3 is installed on the tooling base 1, the replaceable tooling cover plate 3 is pressed on the top surface 13 of the tooling base 1.
[0041] In some embodiments, see Figure 2 The chip positioning portion 12 is two or more chip positioning pins protruding upwardly and arranged on the chip supporting surface 111. The microfluidic chip 2 is formed with two or more chip positioning pin holes 22 corresponding to the two or more chip positioning pins.
[0042] In this embodiment, the microfluidic chip 2 and the tooling base 1 are positioned by tightly inserting the chip positioning pins into the chip positioning pin holes 22, which has a simple structure, fast positioning and convenient operation.
[0043] In a specific embodiment, see Figure 2 The chip positioning pin is perpendicular to the chip supporting surface 111 .
[0044] In one embodiment, see Figure 2 The replaceable tooling cover 3 is detachably connected to the tooling base 1 through the cover positioning pin 14.
[0045] In this embodiment, the replaceable tooling cover plate 3 and the tooling base 1 can be positioned quickly and disassembled conveniently and quickly.
[0046] Specifically, see Figure 2The top surfaces 13 of both ends of the tooling base 1 in the length direction are both provided with cover plate positioning pins 14. The replaceable tooling cover plate 3 is provided with cover plate positioning pin holes 32 corresponding to the cover plate positioning pins 14.
[0047] It should be understood that the positions of the cover plate positioning pin 14 and the cover plate positioning pin hole 32 can also be interchanged. After the interchange, when the replaceable tooling cover 3 is installed on the tooling base 1, the cover plate positioning pin 14 should extend downward into the cover plate positioning pin hole 32 without exceeding.
[0048] In one embodiment, see Figure 2 The replaceable tooling cover plate 3 is also provided with an avoidance through hole 33 for avoiding the chip positioning pin.
[0049] In this embodiment, when the replaceable tooling cover plate 3 is placed on the tooling base 1, if the chip positioning pin is too long, the excessive portion can extend through the corresponding avoidance through hole 33. This avoids the situation where the excessively long chip positioning pin lifts up the replaceable tooling cover plate 3, causing the replaceable tooling cover plate 3 to be unable to be installed on the tooling base 1.
[0050] In a specific embodiment, if Figure 1 As shown, in the microfluidic chip 2, there are 6 detection units, which are distributed in a circular array around the rotation center of the microfluidic chip 2, and each detection unit is provided with 5 reaction chambers 21. Between different detection units, the reaction chambers corresponding to the positions are set to be filled with the same reagent. And within the same detection unit, each reaction chamber is set to be able to be filled with different reagents.
[0051] The microfluidic chip 2 should be equipped with five replaceable cover plates 3, which can be used in sequence to achieve the filling of five reaction chambers with different reagents. Figure 4 , if five reaction chambers are filled in a clockwise direction, the five reaction chambers are numbered as the first reaction chamber, the second reaction chamber, etc. in a clockwise direction. Before filling, the microfluidic chip 2 is supported and limited on the tooling base 1, and then the first replaceable cover plate 3-1 is covered on the microfluidic chip 2. Figure 4 As shown, the through hole for injection on the first replaceable cover plate 3-1 corresponds to Figure 4 The first through hole 31-1 in the microfluidic chip 2 is filled into the first reaction chamber of each detection unit on the microfluidic chip 2 through the first through hole 31-1. Then, the first replaceable cover plate 3-1 is replaced with the second replaceable cover plate 3-2. Figure 4 As shown, the through hole for injection on the second replaceable cover plate 3-2 corresponds to Figure 4 The second through hole 31-2 in the microfluidic chip 2 is used to perfuse the second reaction chamber of each detection unit on the microfluidic chip 2. By analogy, the perfusion of the five reaction chambers can be completed.
[0052] The utility model provides a thought and method for a microfluidic chip reagent perfusion positioning error-proof tooling. There are many methods and ways to implement the technical solution. The above is only a preferred implementation of the utility model. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the utility model. These improvements and modifications should also be regarded as the protection scope of the utility model. All components not specified in this embodiment can be implemented by existing technologies.
Claims
1. A microfluidic chip reagent perfusion positioning error-proof tooling, characterized in that: include: A tooling base (1) comprising a chip carrying portion (11) for carrying a microfluidic chip (2) and a chip positioning portion (12) for positioning the microfluidic chip (2); and a replaceable tooling cover plate (3), wherein the replaceable tooling cover plate (3) is provided with a through hole (31) corresponding to the reaction chamber (21) to be filled in the microfluidic chip (2); when the replaceable tooling cover plate (3) is installed on the tooling base (1), the replaceable tooling cover plate (3) covers the microfluidic chip (2) carried on the chip carrying portion (11), and the through hole (31) is directly opposite to the reaction chamber (21) to be filled.
2. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 1, characterized in that: The chip carrying parts (11) are provided with more than two and are arranged in a linear array on the tooling base (1) along the length direction of the tooling base (1).
3. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 1 or 2, characterized in that: The chip carrying portion (11) comprises a chip supporting surface (111) for supporting the microfluidic chip (2) and a receiving space (112) formed above the chip supporting surface (111) for the microfluidic chip (2) to enter and exit, wherein the receiving space (112) is capable of at least partially receiving the microfluidic chip (2).
4. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 3, characterized in that: The chip carrying portion (11) is a groove structure, which is arranged on the top surface (13) of the tooling base (1) and is recessed inwardly; the chip supporting surface (111) is formed at the bottom of the groove structure, and the inner cavity of the groove structure corresponds to the accommodating space (112).
5. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 4, characterized in that: Two opposite side walls of the groove structure are also provided with two notches (113) for clamping and removing the microfluidic chip (2).
6. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 4, characterized in that: The height between the chip support surface (111) and the top surface (13) of the tooling base (1) is less than the height of the microfluidic chip (2); when the replaceable tooling cover (3) is installed on the tooling base (1), the replaceable tooling cover (3) is pressed onto the microfluidic chip (2).
7. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 6, characterized in that: The chip positioning portion (12) is two or more chip positioning pins protruding upwardly and arranged on the chip supporting surface (111); and the microfluidic chip (2) is formed with two or more chip positioning pin holes (22) corresponding to the two or more chip positioning pins.
8. The microfluidic chip reagent perfusion positioning error-proofing tooling according to claim 1, characterized in that: The replaceable tooling cover plate (3) is detachably connected to the tooling base (1) via a cover plate positioning pin (14).
9. A microfluidic chip reagent perfusion positioning error-proofing tool according to claim 8, characterized in that: The tooling base (1) is provided with the cover plate positioning pins (14) on both end top surfaces (13) in the length direction thereof; and the replaceable tooling cover plate (3) is provided with cover plate positioning pin holes (32) corresponding to the cover plate positioning pins (14).
10. The microfluidic chip reagent perfusion positioning error-proofing tool according to claim 7, characterized in that: The replaceable tooling cover plate (3) is also provided with an avoidance through hole (33) for avoiding the chip positioning pin.