Sampling equipment for food microorganism detection

Abstract

The utility model belongs to the field of food detection, in particular relates to sampling equipment for food microorganism detection, and aims to solve the problem that the actual sampling efficiency is reduced because the conventional equipment is relatively single, can only be used for mixing independently in a hand-cranking manner or a mixing device and then is taken and placed in a centrifugal machine for separation. According to the scheme, the device comprises a shell, a cover top is hinged to the top of the shell, an inner barrel is rotationally arranged on the upper portion of the interior of the shell, and a partition plate is fixedly arranged in the inner barrel; the multiple placing frames and the multiple knuckle bearings are used for placing reagent tubes, the multiple placing frames are all located in the inner cylinder body and are annularly arranged, and the multiple reagent tubes can be driven to rotate and be mixed through the mixing assembly; through the separation assembly, microbial cells, fragments or related substances can be separated from supernate, the detection sensitivity and accuracy are improved, and the practicability and the sampling efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of food detection, in particular to a sampling device for food microorganism detection. Background Art

[0002] In the field of food safety, microbial testing is a key link in ensuring food quality and safety. Food microbial testing can timely detect pathogenic bacteria and spoilage bacteria in food, thereby effectively preventing the occurrence of foodborne diseases and protecting the health of consumers.

[0003] During the sampling step, a deep extractant is first added to the crushed food to allow it to react with the microbial cells to help extract the DNA or RNA in the microbial cells. In order to reduce interference factors and improve accuracy, a centrifuge is used to separate the microbial cells, fragments or related substances from the supernatant (such as culture medium, water, etc.) through centrifugal force. The existing equipment is relatively simple and can only be mixed by hand or mixing equipment, and then placed in a centrifuge for separation, which reduces the actual sampling efficiency. Utility Model Content

[0004] The purpose of the utility model is to solve the problem that the equipment in the prior art is relatively simple and can only be mixed by hand-cranking or mixing equipment, and then placed in a centrifuge for separation, thereby reducing the actual sampling efficiency. A sampling device for food microbial detection is proposed to solve the problem that the equipment in the prior art is relatively simple and can only be mixed by hand-cranking or mixing equipment, and then placed in a centrifuge for separation, thereby reducing the actual sampling efficiency.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A sampling device for food microorganism detection comprises a shell, a cover is hingedly provided on the top of the shell, an inner cylinder is rotatably provided on the upper part of the shell, and a partition is fixedly provided inside the inner cylinder;

[0007] A plurality of placement racks and a plurality of joint bearings are used to place reagent tubes, the plurality of placement racks are all located inside the inner cylinder and are arranged in a ring shape, a plurality of placement holes are opened on the top of the inner cylinder, the plurality of joint bearings are respectively arranged inside the plurality of placement holes, and the plurality of joint bearings are respectively matched with the plurality of placement racks;

[0008] A mixing component, used for mixing the food sample and the extractant in the reagent tube, wherein the mixing component is arranged inside the inner cylinder;

[0009] The separation component is used to separate the mixed sample, and the separation component is arranged on the bottom inner wall of the shell.

[0010] In a possible design, the placement rack is arranged at an angle.

[0011] In a possible design, the joint bearing includes an outer ring and an inner ring, the two ends of the outer wall of the outer ring are symmetrically rotatably arranged inside the placement hole, the two ends of the outer wall of the inner ring are symmetrically rotatably arranged inside the outer ring, and the rotation points of the inner ring and the outer ring are cross-arranged.

[0012] In a possible design, the mixing assembly includes a plurality of limit plates, which are arranged in a ring and fixed on the top of the partition, a rotating rod is rotatably provided at the center of the limit plates, a connecting plate is fixedly provided on the outer wall of the rotating rod, a side plate is fixedly provided at one end of the connecting plate, and the top end of the side plate is fixedly provided at the bottom of the placement rack.

[0013] In a possible design, the mixing assembly also includes a No. 1 motor fixedly arranged on the bottom inner wall of the shell, the output end of the No. 1 motor is connected to a No. 1 connecting rod, the top end of the No. 1 connecting rod penetrates into the interior of the inner cylinder, the bottom end of the rotating rod rotates and penetrates the limit plate and the partition, the outer wall of the rotating rod and the outer wall of the No. 1 connecting rod are fixedly sleeved with a No. 1 gear, and the multiple rotating rods and the No. 1 connecting rod are connected through the No. 1 gear transmission.

[0014] In one possible design, the separation assembly includes a No. 2 motor fixedly mounted on the bottom inner wall of the shell, a No. 2 connecting rod fixedly mounted on the output shaft of the No. 2 motor, a cylinder fixedly mounted at the bottom center of the inner cylinder, and a No. 1 connecting rod located inside the cylinder, and a No. 2 gear fixedly mounted on the outer wall of the cylinder and the outer wall of the No. 2 connecting rod, and the two No. 2 gears are meshed with each other.

[0015] In a possible design, a groove is formed at the bottom end of the No. 1 connecting rod, an overrunning clutch is sleeved on the outer wall of the No. 1 motor output shaft, and the outer wall of the overrunning clutch is arranged on the inner wall of the groove.

[0016] In the present application, when used specifically, after placing the reagent tube containing food and extractant on the joint bearing and the placement rack, the No. 1 motor is driven, the No. 1 motor drives the No. 1 connecting rod to rotate, the No. 1 connecting rod drives the rotating rod to rotate through the No. 1 gear, and the rotating rod drives the placement rack to rotate through the connecting plate and the side plate, thereby driving the bottom of the reagent tube to rotate and shake, and the joint bearing can cooperate with the rotation of the reagent tube to achieve a mixing effect, so that the agent reacts with the substance in the microbial cell, thereby helping to extract the genetic material of the microorganism;

[0017] When separation is required, the No. 2 motor is driven, and the No. 2 motor drives the No. 2 connecting rod to rotate. The No. 2 connecting rod drives the cylinder to rotate through the No. 2 gear, and the cylinder drives the inner cylinder to rotate, thereby driving the reagent tube inside the inner cylinder to rotate. The centrifugal force generated by the rotation can achieve the effect of separating different components in the sample due to differences in density or mass. The rotation direction of the No. 2 motor is opposite to that of the No. 1 motor, so that when driving the rotation of the inner cylinder, it will affect the rotation of the No. 1 connecting rod. At this time, the overrunning clutch can be used to prevent the output shaft of the No. 1 motor from rotating when the inner cylinder rotates.

[0018] In the utility model, the sampling device for food microorganism detection can achieve, through the mixing component, after the loaded reagent tube is placed in the placement rack, multiple reagent tubes can be driven to rotate by driving the No. 1 motor, so as to mix the food sample with the deep extractant, so that the agent reacts with the substances in the microbial cells to help extract the genetic material of the microorganism;

[0019] In the utility model, the sampling device for food microorganism detection can achieve the separation of microbial cells, fragments or related substances from the supernatant of the mixed sample by centrifugal force through the separation component, and then remove the supernatant, thereby reducing interference factors, improving the sensitivity and accuracy of detection, and better completing the sampling steps;

[0020] In the utility model, after the loaded reagent tubes are placed on the placement rack, the mixing assembly can drive multiple reagent tubes to rotate and mix;

[0021] Through the separation component, microbial cells, fragments or related substances can be separated from the supernatant, thereby improving the sensitivity and accuracy of the detection. Ultimately, it is possible to achieve different links in the sampling process by simply driving the corresponding motor, thereby increasing practicality and sampling efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the main structure of a sampling device for food microbiological detection proposed by the utility model;

[0023] Figure 2 This is a schematic cross-sectional structure diagram of a sampling device for food microbiological detection proposed by the utility model;

[0024] Figure 3 For this utility model Figure 2 A magnified view of the structure of part A;

[0025] Figure 4 This is a schematic cross-sectional structure diagram of a sampling device for food microbiological detection proposed by the utility model;

[0026] Figure 5 The utility model provides a three-dimensional structural schematic diagram of a joint bearing of a sampling device for food microorganism detection.

[0027] In the figure: 1. Shell; 2. Inner cylinder; 3. Partition; 4. Motor No. 1; 5. Connecting rod No. 1; 6. Gear No. 1; 7. Rotating rod; 8. Connecting plate; 9. Side plate; 10. Placement rack; 11. Limiting plate; 12. Spherical bearing; 13. Motor No. 2; 14. Gear No. 2; 15. Cylinder; 16. Inner ring; 17. Outer ring; 18. Placement hole; 19. Overrunning clutch; 20. Connecting rod No. 2. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0029] Example 1

[0030] Reference Figure 1-5 A sampling device is used in the field of food testing, comprising: a shell 1, an inner cylinder 2, a partition 3, a placement rack 10, a spherical bearing 12, a mixing component and a separation component.

[0031] The top of the shell 1 is hinged with a cover for easy opening and closing. The inner cylinder 2 is arranged above the interior of the shell 1 and can rotate therein. The partition 3 is fixedly arranged inside the inner cylinder 2 to separate the space of the inner cylinder 2.

[0032] Reference Figure 4 A plurality of placement racks 10 are arranged in a ring shape inside the inner cylinder 2 for placing reagent tubes. The placement racks 10 are arranged at an angle to facilitate subsequent rotation and mixing. A plurality of placement holes 18 are provided on the top of the inner cylinder 2. The articulated bearings 12 are arranged inside the placement holes 18 and cooperate with the placement racks 10 to realize the placement of reagent tubes and the rotation of the placement racks 10.

[0033] Reference Figure 5 The spherical bearing 12 includes an outer ring 17 and an inner ring 16. The outer wall of the outer ring 17 has two symmetrical ends that are rotatably arranged inside the placement hole 18, and the outer wall of the inner ring 16 has two symmetrical ends that are rotatably arranged inside the outer ring 17. The rotation points of the inner ring 16 and the outer ring 17 are arranged crosswise. This structure enables the spherical bearing 12 to rotate in multiple directions.

[0034] Reference Figure 2The mixing assembly is used to mix the food sample and the extractant in the reagent tube, and the mixing assembly includes a plurality of limiting plates 11, a rotating rod 7, a connecting plate 8 and a side plate 9. The limiting plates 11 are arranged in a ring and fixedly arranged on the top of the partition 3, the rotating rod 7 is rotatably arranged at the center of the limiting plates 11, one end of the connecting plate 8 is fixedly arranged on the outer wall of the rotating rod 7, and the other end is fixedly arranged on the top of the side plate 9, and the bottom end of the side plate 9 is fixedly arranged on the bottom of the placement rack 10;

[0035] When the rotating rod 7 rotates, the placement rack 10 is driven to rotate through the connecting plate 8 and the side plate 9, thereby driving the bottom of the reagent tube to rotate and shake, and the joint bearing 12 can cooperate with the rotation of the reagent tube to achieve a mixing effect, so that the medicine reacts with the substances in the microbial cells, thereby helping to extract the genetic material of the microorganism.

[0036] In order to drive the rotating rod 7 to rotate, the mixing assembly also includes a No. 1 motor 4, a No. 1 connecting rod 5 and a No. 1 gear 6. The No. 1 motor 4 is fixedly arranged on the bottom inner wall of the housing 1, and its output end is connected to the No. 1 connecting rod 5. The top end of the No. 1 connecting rod 5 penetrates into the interior of the inner cylinder 2 and is rotatably connected to the bottom end of the rotating rod 7. The outer walls of the rotating rod 7 and the outer walls of the No. 1 connecting rod 5 are both fixedly sleeved with a No. 1 gear 6, and the two No. 1 gears 6 are meshed. When the No. 1 motor 4 is started, the rotating rod 7 is driven to rotate through the No. 1 connecting rod 5 and the No. 1 gear 6.

[0037] Reference Figure 4 , the separation component is used to separate the mixed sample. The separation component includes a No. 2 motor 13, a No. 2 connecting rod 20 and a No. 2 gear 14. The No. 2 motor 13 is fixedly arranged on the bottom inner wall of the shell 1, and its output shaft is fixedly connected to the No. 2 connecting rod 20. A cylinder 15 is fixedly arranged at the bottom center of the inner cylinder 2, and the No. 1 connecting rod 5 is located inside the cylinder 15. The outer wall of the cylinder 15 and the outer wall of the No. 2 connecting rod 20 are both fixedly sleeved with a No. 2 gear 14, and the two No. 2 gears 14 are meshed with each other;

[0038] When the No. 2 motor 13 is started, the No. 2 connecting rod 20 and the No. 2 gear 14 drive the inner cylinder 2 to rotate, thereby driving the reagent tube inside the inner cylinder 2 to rotate, and the microbial cells, fragments or related substances are separated from the supernatant through the centrifugal force generated by the rotation. The supernatant is then removed to reduce interference factors, improve the sensitivity and accuracy of detection, and better complete the sampling steps.

[0039] The present application can be used in the field of food testing, and can also be used in other fields applicable to the present application.

[0040] Example 2

[0041] refer to Figure 3, improved on the basis of Example 1: a sampling device for food microorganism detection, which is applied to the field of food detection, a groove is provided at the bottom end of the No. 1 connecting rod 5, and an overrunning clutch 19 is sleeved on the outer wall of the output shaft of the No. 1 motor 4. The outer wall of the overrunning clutch 19 is arranged on the inner wall of the groove. The function of the overrunning clutch 19 is to prevent the output shaft of the No. 1 motor 4 from rotating when the inner cylinder 2 rotates. Specifically, the rotation direction of the No. 2 motor 13 is opposite to that of the No. 1 motor 4, so that when driving the rotation of the inner cylinder 2, it will affect the rotation of the No. 1 connecting rod 5. At this time, the overrunning clutch 19 can be used to prevent the output shaft of the No. 1 motor 4 from rotating when the inner cylinder 2 rotates.

[0042] However, as is well known to those skilled in the art, the working principles and wiring methods of motor No. 1 4 and motor No. 2 13 are commonplace, and are conventional means or common knowledge, and will not be elaborated here. Those skilled in the art may make any optional selections according to their needs or convenience.

[0043] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A sampling device for food microbiological detection, characterized in that: include: A shell (1), wherein a cover is hingedly provided on the top of the shell (1), an inner cylinder (2) is rotatably provided on the upper part of the shell (1), and a partition (3) is fixedly provided inside the inner cylinder (2); A plurality of placement racks (10) and a plurality of joint bearings (12) are used to place reagent tubes. The plurality of placement racks (10) are all located inside the inner cylinder (2) and are arranged in a ring shape. The top of the inner cylinder (2) is provided with a plurality of placement holes (18). The plurality of joint bearings (12) are respectively arranged inside the plurality of placement holes (18). The plurality of joint bearings (12) are respectively matched with the plurality of placement racks (10); A mixing component, used for mixing the food sample and the extractant in the reagent tube, wherein the mixing component is arranged inside the inner cylinder (2); The separation component is used to separate the mixed sample, and the separation component is arranged on the bottom inner wall of the shell (1).

2. A sampling device for food microorganism detection according to claim 1, characterized in that: The placement rack (10) is arranged in an inclined manner.

3. A sampling device for food microorganism detection according to claim 2, characterized in that: The joint bearing (12) comprises an outer ring (17) and an inner ring (16), wherein two symmetrical ends of the outer wall of the outer ring (17) are rotatably arranged inside the placement hole (18), and two symmetrical ends of the outer wall of the inner ring (16) are rotatably arranged inside the outer ring (17), and the rotation points of the inner ring (16) and the outer ring (17) are arranged crosswise.

4. A sampling device for food microorganism detection according to claim 3, characterized in that: The mixing assembly comprises a plurality of limiting plates (11), which are arranged in a ring shape and fixedly arranged on the top of the partition (3), a rotating rod (7) is rotatably arranged at the center of the limiting plates (11), a connecting plate (8) is fixedly arranged on the outer wall of the rotating rod (7), a side plate (9) is fixedly arranged at one end of the connecting plate (8), and the top end of the side plate (9) is fixedly arranged at the bottom of the placement rack (10).

5. A sampling device for food microorganism detection according to claim 4, characterized in that: The mixing assembly further comprises a No. 1 motor (4) fixedly arranged on the inner wall of the bottom of the shell (1); the output end of the No. 1 motor (4) is connected to a No. 1 connecting rod (5); the top end of the No. 1 connecting rod (5) penetrates into the interior of the inner cylinder (2); the bottom end of the rotating rod (7) rotates to penetrate the limit plate (11) and the partition (3); the outer wall of the rotating rod (7) and the outer wall of the No. 1 connecting rod (5) are both fixedly sleeved with a No. 1 gear (6); and the plurality of rotating rods (7) and the No. 1 connecting rod (5) are all connected in transmission via the No. 1 gear (6).

6. A sampling device for food microorganism detection according to claim 5, characterized in that: The separation assembly comprises a No. 2 motor (13) fixedly arranged on the inner wall of the bottom of the shell (1), a No. 2 connecting rod (20) fixedly arranged on the output shaft of the No. 2 motor (13), a cylinder (15) fixedly arranged at the bottom center of the inner cylinder (2), and a No. 1 connecting rod (5) located inside the cylinder (15), and a No. 2 gear (14) fixedly sleeved on the outer wall of the cylinder (15) and the outer wall of the No. 2 connecting rod (20), and the two No. 2 gears (14) are meshed with each other.

7. A sampling device for food microorganism detection according to claim 6, characterized in that: A groove is provided at the bottom end of the No. 1 connecting rod (5), and an overrunning clutch (19) is sleeved on the outer wall of the output shaft of the No. 1 motor (4), and the outer wall of the overrunning clutch (19) is arranged on the inner wall of the groove.

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