Metabolin continuous monitoring system

Abstract

The utility model provides a metabolite continuous monitoring system which comprises a sample injection pipeline, a flow cell, sensing equipment, analysis equipment and a return pipeline, the sample injection pipeline is connected with the bottom of the flow cell, the return pipeline is connected with the top of the flow cell, the sensing equipment is arranged in the flow cell, and the analysis equipment is in communication connection with the sensing equipment; the sample introduction pipeline is used for introducing fermentation liquor in the fermentation tank into the flow cell; the sensing equipment is used for detecting the fermentation liquor in the flow cell to obtain detection data; the analysis equipment is used for acquiring the detection data from the sensing equipment and analyzing the detection data to obtain a detection result; and the return pipeline is used for guiding the detected fermentation liquor back to the fermentation tank. The metabolite continuous monitoring system is designed to replace manual detection, the detection period can be greatly shortened, the detection efficiency is improved, and the detection digitization and intelligentization level is improved.

Description

Technical Field

[0001] The present application relates to the field of continuous fermentation technology, and in particular to a metabolite continuous monitoring system. Background Art

[0002] Gas biofermentation technology, especially the biofermentation technology that uses industrial tail gas containing carbon monoxide, hydrogen and carbon dioxide as the gas source and produces ethanol and clostridial protein through continuous fermentation by microorganisms, is being rapidly promoted and applied in the industry. In the continuous fermentation process, in order to ensure the continuous and stable operation of the fermentation, ensure the high metabolic activity of the bacteria, and produce as many target metabolites as possible, it is necessary to continuously detect the metabolites of the raw liquid. At present, the main detection scheme is manual sampling, and the use of liquid chromatography, microplate reader, etc. for analysis. Because the number of samples to be tested is large, there are problems such as long detection cycle (the interval between sample results of a single tank is about 2-3 hours) and low detection efficiency. Utility Model Content

[0003] The present application provides a metabolite continuous monitoring system that can significantly reduce the detection cycle, improve detection efficiency, and enhance the level of digitalization and intelligence of detection.

[0004] The present application provides a metabolite continuous monitoring system, comprising: an injection pipeline, a circulation cell, a sensor device, an analysis device and a return pipeline, wherein the injection pipeline is connected to the bottom of the circulation cell, the return pipeline is connected to the top of the circulation cell, the sensor device is arranged in the circulation cell, and the analysis device is communicatively connected to the sensor device;

[0005] The sample introduction line is used to introduce the fermentation liquid in the fermentation tank into the circulation cell;

[0006] The sensor device is used to detect the fermentation liquid in the circulation pool to obtain detection data;

[0007] The analysis device is used to obtain the detection data from the sensor device and analyze the detection data to obtain the detection result;

[0008] The return pipeline is used to guide the detected fermentation liquid back to the fermentation tank.

[0009] Optionally, the metabolite continuous monitoring system further comprises a bypass line, wherein the bypass line is connected to the injection line and the return line respectively;

[0010] The bypass line is used to guide the detected fermentation liquid back to the fermentation tank when the detection of the fermentation liquid in the fermentation tank is stopped.

[0011] Optionally, the sample inlet line, the return line and the bypass line are respectively provided with automatic valves;

[0012] The automatic valve is used to control the flow direction of the fermentation liquid.

[0013] Optionally, the bypass line is also used to guide the tested fermentation liquid back to the fermenter when the automatic valves on the injection line and the return line are closed. The bypass line is arranged beside the circulation pool. During normal testing, the fermentation liquid flowing out of the fermenter passes through the circulation pool and then returns to the fermenter from the return line. When it is determined to stop the test, the fermentation liquid flowing out of the fermenter passes through the circulation pool and then enters the bypass line and then flows back to the fermenter through the bypass line. Optionally, the injection line and the return line are respectively provided with check valves;

[0014] The check valve is used to prevent the fermentation liquid from flowing back.

[0015] Optionally, the top and bottom of the circulation pool are respectively provided with hand valves;

[0016] The hand valve is used to clean the circulation pool.

[0017] Optionally, the types of the analysis equipment include at least: a near infrared analyzer and a Raman detection analyzer.

[0018] Optionally, the return pipeline is also used to guide the fermentation liquid after detection in the circulation pool to a sewage treatment device.

[0019] Optionally, the automatic valve is also used to obtain a PLC control instruction, and perform an opening operation or a closing operation according to the PLC control instruction.

[0020] Optionally, the sensing device is a fiber optic sensor, and the fiber optic sensor is arranged on a side wall of the circulation pool.

[0021] The present application provides a metabolite continuous monitoring system, including: an injection line, a circulation cell, a sensor device, an analysis device and a return line, wherein the injection line is connected to the bottom of the circulation cell, the return line is connected to the top of the circulation cell, the sensor device is arranged in the circulation cell, and the analysis device is connected to the sensor device for communication; the injection line is used to introduce the fermentation liquid in the fermentation tank into the circulation cell; the sensor device is used to detect the fermentation liquid in the circulation cell to obtain detection data; the analysis device is used to obtain detection data from the sensor device, and analyze the detection data to obtain detection results; the return line is used to guide the detected fermentation liquid back to the fermentation tank. The present application replaces manual detection by designing a system for detecting metabolites of continuous gas fermentation, which can greatly reduce the detection cycle and improve the detection efficiency. It has multiple advantages such as improving the level of digitalization and intelligence of detection, reducing the workload of manual operation, and reducing labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 It is a schematic diagram of the structure of a system for detecting metabolites of continuous gas-containing fermentation shown in one embodiment of the present application;

[0024] Among them, 1-injection pipeline; 2-circulation pool; 3-sensing equipment; 4-host; 5-return pipeline; 6-bypass pipeline; 7-automatic valve No. 1; 8-automatic valve No. 2; 9-automatic valve No. 3; 10-check valve No. 1; 11-check valve No. 2; 12-manual valve No. 1; 13-manual valve No. 2. DETAILED DESCRIPTION

[0025] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application is further described in detail below in conjunction with the accompanying drawings and specific implementation methods. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0026] The present application provides a continuous monitoring system for metabolites, such as Figure 1 shown. Figure 1 1 is a schematic diagram of a system for detecting metabolites of continuous gas-containing fermentation according to an embodiment of the present application. Figure 1The system of the present application includes: an injection pipeline 1, a circulation pool 2, a sensor device 3, an analysis device and a return pipeline 5. The injection pipeline 1 is connected to the bottom of the circulation pool 2, the return pipeline 5 is connected to the top of the circulation pool 2, the sensor device 3 is arranged in the circulation pool 2, and the analysis device is connected to the sensor device 3 in communication.

[0027] Among them, the sampling pipeline 1 is used to introduce the fermentation liquid (including the metabolites to be detected) in the fermentation tank into the circulation pool 2; the sensor device 3 is used to detect the fermentation liquid in the circulation pool 2 to obtain detection data; the analysis device is used to obtain the detection data from the sensor device 3, and analyze the detection data to obtain the detection result; the return pipeline 5 is used to guide the detected fermentation liquid back to the fermentation tank. In this embodiment, the fermentation liquid in the fermentation tank enters through the sampling pipeline 1 and enters the circulation pool 2 from the bottom to the top from the bottom of the circulation pool 2. In the circulation pool 2, the sensor device 3 detects the fermentation liquid, obtains the detection data, and transmits the detection data to the analysis device, and finally the analysis device analyzes the detection data to obtain the detection result of the fermentation liquid. The fermentation liquid after detection flows into the return pipeline 5 from the top of the circulation pool 2, and finally flows back to the fermentation tank.

[0028] The fermentation broth in this embodiment refers to a liquid culture medium in which microorganisms are inoculated. After a period of cultivation, the microorganisms utilize the nutrients in the culture medium to synthesize bacteria and secrete products. The liquid metabolized by the microorganisms is called fermentation broth.

[0029] In one embodiment, the fermentation liquid in this example is mash, which is a colored solution containing bacteria and various metabolites produced by microbial fermentation.

[0030] In one embodiment, the system may further include a host 4 (the host 4 is equipped with a computer, such as Figure 1 The host 4 is connected to the sensing device 3 and the analysis device respectively, and the host 4 is used to control the detection process of the entire system, including controlling the sensing device 3 and the analysis device. The complete process of the analysis device obtaining the detection result according to the detection data includes: the analysis device first obtains the spectral signal according to the detection data collected by the sensing device 3, and sends the spectral signal to the host 4. The host 4 has a detection model, and different models can be selected for different samples and the spectral signal is converted into a detection result. The user can view the detection result through the computer.

[0031] In this embodiment, the fermentation liquid continuously enters the circulation pool 2 through the sampling pipeline 1, and then returns to the fermentation tank through the return pipeline 5. In this process, the sensor device 3 continuously detects and obtains detection data, thereby improving the detection efficiency.

[0032] In this embodiment, the system is designed to replace manual testing of the fermentation liquid, which can greatly reduce the detection cycle of metabolites (generally the detection cycle of a single fermentation tank can be reduced to 10 to 20 minutes), significantly improve the detection efficiency, and provide more timely and accurate guidance for subsequent production control and adjustment, thereby solving the problem of lag in monitoring the fermentation process.

[0033] In combination with the above embodiments, in one implementation, the circulation pool 2 is a common passage of a multi-channel detection device. Therefore, the volume of the circulation pool 2 should be as small as possible on the basis of meeting the distance before and after the access point of the sensor device 3 to the circulation pool sensor device, so as to reduce the replacement time after switching the sampling point.

[0034] In combination with the above embodiments, in one embodiment, the system may further include a bypass line 6, which is disposed beside the circulation pool 2, and the bypass line 6 is respectively connected to the inlet end of the injection line 1 and the outlet end of the return line 5, and the specific connection relationship is as follows: Figure 1 The sample injection line 1, the return line 5 and the bypass line 6 form a detection line for the fermenter, and the detection line plus the circulation pool 2 form a complete detection loop.

[0035] In various embodiments of the present application, the fermentation liquid after detection refers to: all the fermentation liquid that has flowed out of the fermentation tank when the detection of the fermentation liquid in the fermentation tank is stopped, that is, the fermentation liquid remaining in the detection circuit.

[0036] When the detection of the fermented liquid in the current fermentation tank is stopped (finished), in order not to affect the circulation pool 2 to continue to detect the fermented liquid in the next fermentation tank, the fermented liquid in the detection loop is first introduced into the bypass pipeline 6, so that the fermented liquid can maintain a continuous circulation state through the bypass pipeline 6, and finally flow back to the fermentation tank through the bypass pipeline 6. In this way, the circulation pool 2 can be quickly used to detect the fermented liquid in the next fermentation tank, which can save the time of replacing the liquid in the pipeline before the next detection. Secondly, when the circulation pool 2 is cleaned on the spot, the loop formed based on the bypass pipeline can also be used as a temporary circulation loop for the fermented liquid.

[0037] In this embodiment, during the normal detection of the fermentation liquid in the fermentation tank by the system, the fermentation liquid flowing out of the fermentation tank passes through the circulation pool 2 and returns to the fermentation tank through the return pipeline 5. When the detection of the fermentation liquid in the current fermentation tank is ended (stopped), or when the fermentation tank needs to be switched to detect the fermentation liquid in other fermentation tanks, the fermentation liquid flowing out of the fermentation tank passes through the circulation pool 2, first enters the bypass pipeline 6, and then flows back to the fermentation tank through the bypass pipeline 6. The bypass pipeline 6 can ensure that the liquid in the pipeline is in a continuous flow state to avoid fluctuations in the metabolite components caused by retention. In combination with the above embodiments, in one embodiment, automatic valves are respectively provided on the injection pipeline 1, the return pipeline 5 and the bypass pipeline 6 to control the flow direction of the fermentation liquid.

[0038] Among them, according to Figure 1 , the injection line 1 is provided with a No. 1 automatic valve 7, the return line 5 is provided with a No. 2 automatic valve 8, and the bypass line 6 is provided with a No. 3 automatic valve 9. The opening sequence or closing sequence of each automatic valve can be determined according to a preset PLC control program.

[0039] In combination with the above embodiments, in one implementation manner, the bypass line 6 is also used to guide the detected fermentation liquid back to the fermentation tank when the automatic valves on the injection line 1 and the return line 5 are closed.

[0040] In this embodiment, when the detection of the fermentation liquid in the current fermentation tank is finished (stopped), or when the fermentation tank is switched to detect the fermentation liquid in other fermentation tanks, the automatic valve 9 on the bypass line 6 is opened first, and then the automatic valve 7 on the injection line 1 and the automatic valve 8 on the return line 5 are closed, so that the fermentation liquid in the detection loop first flows into the bypass line 6 and then returns to the fermentation tank through the bypass line 6.

[0041] In combination with the above embodiments, in one implementation manner, the sample inlet line 1 and the return line 5 are respectively provided with a check valve to prevent the fermentation liquid from flowing back.

[0042] like Figure 1 A No. 1 check valve 10 is arranged on the injection pipeline 1, and a No. 2 check valve 11 is arranged on the return pipeline 5. The check valves can prevent the fermentation liquid from being mixed with each other due to the backflow of the fermentation liquid, thereby avoiding affecting the accuracy of the detection result.

[0043] In combination with the above embodiments, in one implementation manner, the top and bottom of the circulation pool 2 are respectively provided with hand valves for cleaning the circulation pool.

[0044] like Figure 1 A No. 1 hand valve 12 is provided at the top of the circulation pool 2, and a No. 2 hand valve 13 is provided at the bottom of the circulation pool 2, both of which can realize in-situ cleaning and water flushing of the circulation pool 2.

[0045] Specifically, a manual valve 12 is provided at the top of the circulation pool 2, which can also be used to clean the alkali solution pipeline when the vehicle is stopped, and can also be used to perform irregular exhaust operations to prevent the gas of the gas-containing material from accumulating at the top of the circulation pool 2 and avoid affecting the accuracy of the detection results.

[0046] In combination with the above embodiments, in one implementation manner, the types of analysis equipment include at least: a near infrared analyzer and a Raman detection analyzer.

[0047] In this embodiment, the analysis device can use a near-infrared analyzer or a Raman detection analyzer. After establishing a model for different concentration intervals of the fermentation liquid, rapid detection of specific components can be achieved, such as bacterial concentration, alcohols, acids, amino acid components, etc. Of course, the analysis device can also be other types of analyzers, which can be selected according to actual needs, and this embodiment does not limit this.

[0048] In this embodiment, a near-infrared analyzer or a Raman analyzer is used to detect and analyze the fermentation liquid in a continuous fermentation environment, which can greatly reduce the data detection cycle and improve the digitalization and intelligence level of the detection.

[0049] In combination with the above embodiments, in one implementation manner, the return pipeline 5 can also guide the fermentation liquid after detection in the circulation pool 2 to a sewage treatment device.

[0050] In this embodiment, the fermentation liquid can flow into the fermentation tank through the return pipeline 5, and can also flow into a sewage treatment device or a sewage collection device.

[0051] In combination with the above embodiments, in one implementation manner, the automatic valve is further used to obtain a PLC control instruction, and perform an opening operation or a closing operation according to the PLC control instruction.

[0052] In this embodiment, the execution of the opening and closing operations of each automatic valve (including automatic valve 7-automatic valve 8) in the system can be determined according to the PLC control program. Each automatic valve receives the control instruction issued by the PLC device, and responds to the control instruction to perform the opening operation or the closing operation. After performing the opening operation or the closing operation, each automatic valve transmits the execution result to the host 4 to facilitate the next control of the host 4.

[0053] In combination with the above embodiments, in one implementation manner, the sensing device is an optical fiber sensor, and the optical fiber sensor is disposed on a side wall of the circulation pool 2 .

[0054] In this embodiment, the side wall of the flow cell 2 is provided with an optical fiber interface for installing an optical fiber sensor, and the fermentation liquid is detected by the optical fiber sensor to improve the accuracy of the detection data. In actual implementation, other types of sensing devices can also be used, and this embodiment does not specifically limit this.

[0055] For continuous fermentation, the metabolites in the fermentation tank need to be detected in time and continuously. In the present application, the fermented liquid containing the material to be detected in the fermentation tank is transported to the circulation pool 2 by the sampling line 1 and then returned, and the circulation loop (sampling line 1, circulation pool 2, return line 5 and bypass line 6 constitute a circulation loop) of continuous flow is established to realize continuous detection, and the circulation pool 2 is provided with an optical fiber sensor, and the signal is transmitted to the analysis equipment through optical fiber, and the analysis equipment is combined with the main machine 4 to analyze and obtain the test result. Each detection point is in and out of the circulation pool 2 before and after and the bypass line 6 of the circulation pool 2 is provided with an automatic valve, and initialization and sequential control program are designed by PLC. When cutting out the pipeline (stop detecting the fermented liquid in the current fermentation tank), the automatic valve (automatic valve 9) of the bypass line 6 is first opened, and then the automatic valve (automatic valve 7) of the sampling line 1 and the automatic valve (automatic valve 8) on the return line 5 are closed successively, so that the fermented liquid flows back to the fermentation tank by the bypass line 6. For the difference in the concentration of fermentation liquid in different tanks, the host 4 can match the detection model while switching the sequential control, further improving the accuracy of the detection result. At the same time, hand valves are set on the upper and lower sides of the circulation pool 2 and the tank side of each pipeline (injection pipeline 1, return pipeline 5 and bypass pipeline 6), which can realize the cleaning, replacement and exhaust of the pipeline and the circulation pool 2.

[0056] The present application provides a system for detecting metabolites of continuous gas-containing fermentation, including: an injection line, a circulation pool, a sensor device, an analysis device and a return line, wherein the injection line is connected to the bottom of the circulation pool, the return line is connected to the top of the circulation pool, the sensor device is arranged in the circulation pool, and the analysis device is connected to the sensor device for communication; the injection line is used to introduce the fermentation liquid in the fermentation tank into the circulation pool; the sensor device is used to detect the fermentation liquid in the circulation pool to obtain detection data; the analysis device is used to obtain detection data from the sensor device, and analyze the detection data to obtain detection results; the return line is used to guide the detected fermentation liquid back to the fermentation tank. The present application replaces manual detection by designing a system for detecting metabolites of continuous gas-containing fermentation, which can greatly reduce the detection cycle, improve detection efficiency, and has multiple advantages such as improving the level of digitalization and intelligence of detection, reducing the workload of manual operation, and reducing labor costs.

[0057] Although the preferred embodiments of the present application have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present application.

[0058] Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application is also intended to include these modifications and variations.

Claims

1. A metabolite continuous monitoring system, characterized in that: include: An injection pipeline, a circulation pool, a sensor device, an analysis device and a return pipeline, wherein the injection pipeline is connected to the bottom of the circulation pool, the return pipeline is connected to the top of the circulation pool, the sensor device is arranged in the circulation pool, and the analysis device is communicatively connected to the sensor device; The sample introduction line is used to introduce the fermentation liquid in the fermentation tank into the circulation cell; The sensor device is used to detect the fermentation liquid in the circulation pool to obtain detection data; The analysis device is used to obtain the detection data from the sensor device and analyze the detection data to obtain the detection result; The return pipeline is used to guide the detected fermentation liquid back to the fermentation tank.

2. The metabolite continuous monitoring system according to claim 1, characterized in that: The system further comprises a bypass line, wherein the bypass line is connected to the sample inlet line and the return line respectively; The bypass line is used to guide the detected fermentation liquid back to the fermentation tank when the detection of the fermentation liquid in the fermentation tank is stopped.

3. The metabolite continuous monitoring system according to claim 2, characterized in that: The sample inlet pipeline, the return pipeline and the bypass pipeline are respectively provided with automatic valves; The automatic valve is used to control the flow direction of the fermentation liquid.

4. The metabolite continuous monitoring system according to claim 3, characterized in that: The bypass line is also used to guide the detected fermentation liquid back to the fermentation tank when the automatic valves on the sampling line and the return line are closed.

5. The metabolite continuous monitoring system according to claim 1, characterized in that: The injection pipeline and the return pipeline are respectively provided with a check valve; The check valve is used to prevent the fermentation liquid from flowing back.

6. The metabolite continuous monitoring system according to claim 1, characterized in that: The top and bottom of the circulation pool are respectively provided with hand valves; The hand valve is used to clean the circulation pool.

7. The metabolite continuous monitoring system according to claim 1, characterized in that: The types of the analysis equipment include at least: near infrared analyzer and Raman detection analyzer.

8. The metabolite continuous monitoring system according to claim 1, characterized in that: The return pipeline is also used to guide the fermentation liquid detected in the circulation pool to a sewage treatment device.

9. The metabolite continuous monitoring system according to claim 3, characterized in that: The automatic valve is also used to obtain a PLC control instruction and perform an opening operation or a closing operation according to the PLC control instruction.

10. The metabolite continuous monitoring system according to claim 1, characterized in that: The sensing device is an optical fiber sensor, and the optical fiber sensor is arranged on the side wall of the circulation pool.

Images