25-hydroxyvitamin D detection kit

Abstract

The utility model discloses a 25-hydroxyvitamin D detection kit, and particularly relates to the technical field of medical detection kits. The 25-hydroxyvitamin D detection kit mainly comprises a kit body, a detection reagent tube is mounted in the kit body, a first auxiliary detection reagent tube is mounted on one side of the detection reagent tube, and a butt joint detection assembly is arranged on the other side of the detection reagent tube; the butt joint detection assembly comprises a second auxiliary detection reagent tube arranged on the other side of the detection reagent tube, and butt joint conical heads are integrally formed at the top ends of the first auxiliary detection reagent tube and the second auxiliary detection reagent tube through injection molding. According to the utility model, the butt-joint detection assembly is arranged to cut off the tip pipe outside the tip, the butt-joint conical head drives the support ring and the butt-joint backing ring to be in butt joint with the top end of the guide hopper, and the butt-joint backing ring can be firmly attached to the top end of the guide hopper for sealing operation; a small butt-joint opening of the butt-joint conical head is in butt joint with a large opening position in the guide hopper, so that waste of the detection reagent is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of medical detection kits, and more specifically, to a 25-hydroxyvitamin D detection kit. Background Art

[0002] The 25-hydroxyvitamin D detection kit can detect the vitamin D level in the human body, which is helpful for diagnosing vitamin D deficiency, preventing and treating diseases related to vitamin D deficiency such as rickets and osteomalacia; in the prior art, the utility model patent of Chinese Patent Publication No. CN105352958A discloses a total 25-hydroxyvitamin D detection kit, which comprises reagent 2 containing antibodies against human 25-hydroxyvitamin D detection kit 2 and D3 placed in an appropriate buffer, and reagent 3 comprises polystyrene latex particles with human 25-hydroxyvitamin D detection kit 2 and D3 labeled on the surface placed in an appropriate buffer, and has the advantages of high detection sensitivity and accuracy, good precision, wide detection linearity, good stability, short detection time and low production cost; however, the kit still has the following defects during detection;

[0003] When the kit is used for 25-hydroxyvitamin D testing, three reagents need to be added in sequence for mixing. During the dropwise mixing process, since the positions of the tube openings of each reagent are relatively large, it is very easy for the reagent liquid to flow out during the dropwise addition process, resulting in waste of testing reagents. Therefore, a 25-hydroxyvitamin D testing kit is needed. Utility Model Content

[0004] In order to overcome the above-mentioned defects of the prior art, the utility model provides a 25-hydroxyvitamin D detection kit.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a 25-hydroxyvitamin D detection kit, comprising a kit body, a detection reagent tube is installed inside the kit body, a first auxiliary detection reagent tube is installed on one side of the detection reagent tube, and a docking detection assembly is provided on the other side of the detection reagent tube; the docking detection assembly comprises a second auxiliary detection reagent tube arranged on the other side of the detection reagent tube, the top ends of the first auxiliary detection reagent tube and the second auxiliary detection reagent tube are both integrally formed with a docking conical head by injection molding, the top end of the docking conical head is fixedly connected with an outer row of pointed tubes; the outer wall of the docking conical head is fixedly connected with a docking gasket ring and a support ring in sequence from top to bottom;

[0006] The top of the detection reagent tube is fixedly connected to a guide bucket, the first auxiliary detection reagent tube and the second auxiliary detection reagent tube are symmetrically arranged with respect to the detection reagent tube, the two docking conical heads are symmetrically arranged with respect to the guide bucket, the top inner wall diameter of the docking conical head is smaller than the bottom inner wall diameter; the top inner wall diameter of the guide bucket is larger than the bottom inner wall diameter, a cover plate is installed above the reagent kit body, friction blocks are integrally formed on both sides of the cover plate by injection molding, a rotating cap is threadedly connected to the outer wall of the guide bucket, and the top cross-sectional shape of the rotating cap is set to be hexagonal; the bottom ends of the first auxiliary detection reagent tube and the second auxiliary detection reagent tube are fixedly connected with a balloon.

[0007] According to the above technical scheme, the operator rotates the rotating cap to separate the threads between the rotating cap and the guide bucket, and then drops the sample into the guide bucket. The sample can be mixed with the reagent solution inside the detection reagent tube. After the first auxiliary detection reagent tube is moved up and taken out from the inside of the positioning hole plate, the tip of the outer tip tube is cut off, and the opened docking conical head is inserted into the guide bucket. The supporting ring and the docking gasket ring are driven by the docking conical head to dock with the top position of the guide bucket. The docking gasket ring can fit firmly on the top of the guide bucket for sealing operation, and the small mouth of the docking conical head can be docked with the large mouth of the guide bucket, and the docking part can achieve sealing operation.

[0008] Preferably, the outer wall of the detection reagent tube is provided with a positioning hole plate fixedly connected to the reagent kit body, and the detection reagent tube and the first auxiliary detection reagent tube and the second auxiliary detection reagent tube are all vertically slidably connected to the positioning hole plate; a spacing support assembly is installed above the positioning hole plate; the spacing support assembly includes two spacing blocks installed above the positioning hole plate, and the top of the spacing block is provided with a spacing pillar fixedly connected to the support ring, and the spacing pillar and the spacing block are integrated by injection molding; the outer wall of the detection reagent tube is fixedly connected to an upper lifting ring, and a spacing support block and a spacing connecting column are sequentially provided on the outer side of the upper lifting ring from top to bottom, the spacing support block is respectively fixedly connected to the spacing connecting column and the upper lifting ring, and the spacing block and the spacing connecting column are both slidably connected to the positioning hole plate.

[0009] According to the above technical solution, the spacer block provides supporting force for the spacer pillar, and there is a gap between the support ring and the positioning hole plate. The finger is inserted into the gap and moved upward, so that the support ring can drive the spacer pillar to move the spacer block upward. Secondly, the hand can be inserted into the gap between the lifting ring and the positioning hole plate, and the lifting ring drives the spacer support block to move the spacer connecting column upward, which is convenient for moving the detection reagent tube up and out from the inside of the positioning hole plate.

[0010] Technical effects and advantages of the utility model:

[0011] After the tip outer row of the tip tube is cut off by setting the docking detection component, the opened docking cone head is inserted into the guide bucket, and the support ring and the docking gasket ring are driven to dock to the top position of the guide bucket through the docking cone head. The docking gasket ring can be firmly attached to the top of the guide bucket for sealing operation, and the small docking port of the docking cone head is docked to the large opening position inside the guide bucket to avoid the dripping reagent from overflowing and avoid wasting the detection reagent;

[0012] A spacing support assembly is used to enable the spacing support pillar to provide support force to the bottom end of the support ring. There is a gap between the support ring and the positioning hole plate. The finger is inserted into the gap and moved upward. The hand is inserted into the gap between the lifting ring and the positioning hole plate. The lifting ring drives the spacing support block to move the spacing connecting column upward, which is convenient for taking out the detection reagent tube and the first auxiliary detection reagent tube and the second auxiliary detection reagent tube for reagent addition detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the main structure of the 25-hydroxyvitamin D detection kit provided by the utility model.

[0014] Figure 2 It is a schematic diagram of the vertical cross-section structure of the 25-hydroxyvitamin D detection kit of the utility model.

[0015] Figure 3 It is a schematic diagram of the local structure of the vertical section of the connection between the detection reagent tube and the guide bucket of the utility model.

[0016] Figure 4 For the utility model Figure 2 Enlarged structural diagram at A in the middle.

[0017] The accompanying drawings are marked as follows: 1. reagent box body; 2. detection reagent tube; 3. first auxiliary detection reagent tube; 4. second auxiliary detection reagent tube; 5. docking conical head; 6. outer row of pointed tubes; 7. docking gasket; 8. support ring; 9. guide bucket; 10. cover plate; 11. friction block; 12. rotating cap; 13. balloon; 14. positioning hole plate; 15. spacer pillar; 16. spacer block; 17. lifting ring; 18. spacer support block; 19. spacer connecting column. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] As attached Figure 1-4The 25-hydroxyvitamin D detection kit shown in the figure is provided with a docking detection assembly. The setting of the docking detection assembly enables the docking gasket ring 7 to be firmly attached to the top of the guide bucket 9 for sealing operation, and the small docking port of the docking cone head 5 is docked to the large opening position inside the guide bucket 9 to avoid the dripping reagent from overflowing and avoid wasting the detection reagent. The specific structure of the docking detection assembly is set as follows:

[0020] In this embodiment, as shown in the attached Figure 1-4 As shown, the docking detection assembly includes a second auxiliary detection reagent tube 4 arranged on the other side of the detection reagent tube 2, the tops of the first auxiliary detection reagent tube 3 and the second auxiliary detection reagent tube 4 are both integrally formed with a docking cone head 5 by injection molding, and the top of the docking cone head 5 is fixedly connected with an outer row of sharp tubes 6; the outer wall of the docking cone head 5 is fixedly connected with a docking gasket ring 7 and a support ring 8 in sequence from top to bottom; the top of the detection reagent tube 2 is fixedly connected with a guide bucket 9;

[0021] The working principle of the 25-hydroxyvitamin D detection kit in this embodiment is as follows;

[0022] During the test, the operator rotates the rotating cap 12 to separate the threads between the rotating cap 12 and the guide bucket 9, and then drips the sample into the guide bucket 9. The sample can be mixed with the reagent solution in the test reagent tube 2. The reagent in the test reagent tube 2 is 50mM (mmoL / L), pH 4.0 citric acid-trisodium citrate buffer, 0.05% (v / v) (Tween-20 itself is a liquid, V / V means 0.05-0.5ml Tween-20 corresponds to 99.95-99.5ml reagent 1) Tween 20, 1mg / mL Proclin-300 preservative is an added component of the reagent 1 that constitutes the complete acidic dissociation solution. After the first auxiliary detection reagent tube 3 is moved up and taken out from the inside of the positioning hole plate 14, the tip outer row of sharp tubes 6 are cut off, and the opened docking conical head 5 is inserted into the inside of the guide bucket 9. The supporting ring 8 and the docking gasket ring 7 are driven to dock to the top position of the guide bucket 9 through the docking conical head 5. The docking gasket ring 7 can be firmly attached to the top of the guide bucket 9 for sealing. The second reagent in the first auxiliary detection reagent tube 3 is poured into the detection reagent tube 2. The second reagent is an anti-human 25-hydroxyvitamin D detection kit 2 and the D3 antibody is a monoclonal antibody or a polyclonal antibody, wherein the polyclonal antibody is derived from rabbit, sheep or chicken, and the monoclonal antibody is derived from mouse or rabbit; preferably, the anti-human 25-hydroxyvitamin D detection kit 2 and D3 antibodies are monoclonal antibodies. After squeezing the balloon 13, pressure is generated inside the first auxiliary detection reagent tube 3 to quickly squeeze out the second reagent. Secondly, the second auxiliary detection reagent tube 4 and the third reagent are connected to the inside of the guide bucket 9 according to the adding method of the first auxiliary detection reagent tube 3 to achieve plug-in and dripping. The third reagent is a polystyrene latex particle with a particle size of 50nm and a final concentration of 2mg / ml in the reagent 3. After the three reagents are mixed inside the detection reagent tube 2, they are removed for testing to obtain the test data. Since the above three reagents and detection methods need to refer to reference references, they belong to the existing public technology and are therefore not described in detail. Therefore, the small docking port of the docking conical head 5 is docked to the large opening inside the guide bucket 9. Secondly, the guide bucket 9 can fit and seal with the docking gasket ring 7, so as to avoid overflow when adding liquid and avoid waste of reagents.

[0023] In this embodiment, as shown in the attached Figure 1-2As shown, a cover plate 10 is installed on the top of the reagent box body 1, and friction blocks 11 are integrally formed on both sides of the cover plate 10 by injection molding, so that when the reagent box body 1 is assembled, the friction block 11 is pressed downward to drive the cover plate 10 and the reagent box body 1 to be plugged in. Later, the friction block 11 can be pulled up to drive the cover plate 10 and the reagent box body 1 to be separated. The outer wall of the guide bucket 9 is threadedly connected with a rotating cap 12, and the cross-sectional shape of the top of the rotating cap 12 is set to be hexagonal; the bottom ends of the first auxiliary detection reagent tube 3 and the second auxiliary detection reagent tube 4 are fixedly connected with a balloon 13, so that when the reagent inside the first auxiliary detection reagent tube 3 is squeezed, pressing the balloon 13 can generate a large pressure inside the first auxiliary detection reagent tube 3 and quickly discharge the reagent liquid.

[0024] In this embodiment, as shown in the attached Figure 2-4 As shown, the outer wall of the detection reagent tube 2 is provided with a positioning hole plate 14 fixedly connected to the reagent kit body 1, and the detection reagent tube 2 and the first auxiliary detection reagent tube 3 and the second auxiliary detection reagent tube 4 are vertically slidably connected to the positioning hole plate 14; a spacing support assembly is installed above the positioning hole plate 14; the spacing support assembly includes two spacing blocks 16 installed above the positioning hole plate 14, and the top of the spacing block 16 is provided with a spacing pillar 15 fixedly connected to the support ring 8, and the spacing pillar 15 and the spacing block 16 are integrally formed by injection molding;

[0025] The outer wall of the detection reagent tube 2 is fixedly connected with an upper lifting ring 17, and a spacer support block 18 and a spacer connecting column 19 are sequentially arranged from top to bottom on the outer side of the upper lifting ring 17. The spacer support block 18 is fixedly connected to the spacer connecting column 19 and the upper lifting ring 17 respectively, and the spacer block 16 and the spacer connecting column 19 are both slidably connected to the positioning hole plate 14;

[0026] According to the upper structure, when the docking conical head 5 is removed, the spacer block 16 provides support force to the spacer pillar 15, and the spacer pillar 15 provides support force to the bottom end of the support ring 8, so that there is a gap between the support ring 8 and the positioning hole plate 14. The finger is inserted into the gap and moved upward, so that the support ring 8 can drive the spacer pillar 15 to move the spacer block 16 upward, which is convenient for moving the first auxiliary detection reagent tube 3 up and out from the inside of the positioning hole plate 14. Secondly, the hand can be inserted into the gap between the lifting ring 17 and the positioning hole plate 14, and the lifting ring 17 is moved upward. The lifting ring 17 drives the spacer support block 18 to move the spacer connecting column 19 upward, which is convenient for moving the detection reagent tube 2 up and out from the inside of the positioning hole plate 14.

[0027] The contents not described in detail in the specification belong to the prior art known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used. In this technical solution, the electrical control components not mentioned are not shown in the figure because they belong to the prior art and will not be described here.

[0028] Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A 25-hydroxyvitamin D detection kit, comprising a kit body (1), a detection reagent tube (2) installed inside the kit body (1), a first auxiliary detection reagent tube (3) installed on one side of the detection reagent tube (2), characterized in that: The other side of the detection reagent tube (2) is provided with a docking detection component; The docking detection assembly comprises a second auxiliary detection reagent tube (4) arranged on the other side of the detection reagent tube (2); the top ends of the first auxiliary detection reagent tube (3) and the second auxiliary detection reagent tube (4) are both integrally formed with a docking conical head (5) by injection molding; the top ends of the docking conical heads (5) are fixedly connected to an outer row pointed tube (6); The outer wall of the butt-jointed conical head (5) is fixedly connected with a butt-jointed gasket ring (7) and a support ring (8) in sequence from top to bottom; The top end of the detection reagent tube (2) is fixedly connected to a guide bucket (9).

2. The 25-hydroxyvitamin D detection kit according to claim 1, wherein: The first auxiliary detection reagent tube (3) and the second auxiliary detection reagent tube (4) are symmetrically arranged with respect to the detection reagent tube (2), and the two docking conical heads (5) are symmetrically arranged with respect to the guide bucket (9).

3. The 25-hydroxyvitamin D detection kit according to claim 1, characterized in that: The inner wall diameter of the top end of the butting conical head (5) is smaller than the inner wall diameter of the bottom end; The inner wall diameter of the top end of the guide bucket (9) is larger than the inner wall diameter of the bottom end thereof.

4. The 25-hydroxyvitamin D detection kit according to claim 1, characterized in that: A cover plate (10) is installed above the test kit body (1), and friction blocks (11) are integrally formed on both sides of the cover plate (10) by injection molding.

5. The 25-hydroxyvitamin D detection kit according to claim 1, wherein: The outer wall of the guide bucket (9) is threadedly connected to a rotating cap (12), and the cross-sectional shape of the top end of the rotating cap (12) is set to be hexagonal; The bottom ends of the first auxiliary detection reagent tube (3) and the second auxiliary detection reagent tube (4) are both fixedly connected to a balloon (13).

6. The 25-hydroxyvitamin D detection kit according to claim 1, characterized in that: The outer wall of the detection reagent tube (2) is provided with a positioning hole plate (14) fixedly connected to the reagent box body (1), and the detection reagent tube (2), the first auxiliary detection reagent tube (3), and the second auxiliary detection reagent tube (4) are all vertically slidably connected to the positioning hole plate (14); A spacing support assembly is installed above the positioning hole plate (14); The spacing support assembly comprises two spacing blocks (16) mounted above the positioning hole plate (14), the top of each spacing block (16) being provided with a spacing support (15) fixedly connected to the support ring (8), and the spacing support (15) and the spacing block (16) being integrally formed by injection molding; The outer wall of the detection reagent tube (2) is fixedly connected to a lifting ring (17), and a spacer support block (18) and a spacer connecting column (19) are sequentially provided on the outer side of the lifting ring (17) from top to bottom.

7. The 25-hydroxyvitamin D detection kit according to claim 6, characterized in that: The spacer support block (18) is fixedly connected to the spacer connecting column (19) and the lifting ring (17), respectively, and the spacer block (16) and the spacer connecting column (19) are slidably connected to the positioning hole plate (14).

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