Micro-fluidic chip and micro-fluidic chip system

Abstract

The utility model relates to a micro-fluidic chip and a micro-fluidic chip system.The micro-fluidic chip comprises a substrate and a cover plate, the substrate is provided with a conveying flow channel, a liquid bag cavity and a freeze-drying cavity, the conveying flow channel communicates with the liquid bag cavity and the freeze-drying cavity, and the freeze-drying cavity is used for containing freeze-drying balls; the cover plate and the base plate are bonded to seal the freeze-drying cavity and the conveying flow channel, a laying area is arranged on the side, away from the base plate, of the cover plate and used for allowing the liquid bags to be arranged, and part of the laying area is communicated with fluid in the liquid bag cavity so that liquid reagents in the liquid bags can circulate. Due to the fact that the conveying flow channel is communicated with the freeze-drying cavity and the liquid bag cavity, the liquid reagent in the liquid bag can flow into the liquid bag cavity and flow into the freeze-drying cavity through the liquid bag cavity and the conveying flow channel, and the freeze-drying balls are dissolved and react with the freeze-drying reagent. The micro-fluidic chip is provided with the freeze-drying ball and the liquid bag in advance, and the liquid reagent can react with the freeze-drying ball by pressing the liquid bag, so that the operation of the micro-fluidic chip is simplified, and automation is realized conveniently. The micro-fluidic chip system comprises the micro-fluidic chip and is simpler to operate.

Description

Technical Field

[0001] The present application relates to the technical field of microfluidic chips, and in particular to a microfluidic chip and a microfluidic chip system. Background Art

[0002] Microfluidics is a technology that integrates the basic operating units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis process into a micrometer-scale chip and completes the entire analysis process. Microfluidics has the advantages of low production cost, small amount of detection reagents, and fast detection speed. Therefore, it has many applications in medical diagnosis, drug development, chemical testing and other fields.

[0003] However, when using current microfluidic chips, dry and wet reagents need to be introduced externally, which makes the operation of microfluidic chips complicated and inconvenient to automate. Utility Model Content

[0004] Based on this, it is necessary to provide a microfluidic chip and a microfluidic chip system to address the problem of complex operation of current microfluidic chips.

[0005] The present application provides a microfluidic chip, which includes a substrate and a cover plate. The substrate is provided with a transport channel, a liquid capsule cavity and a freeze-drying cavity. The transport channel connects the liquid capsule cavity and the freeze-drying cavity respectively, and the freeze-drying cavity is used to accommodate freeze-dried balls; the cover plate is bonded to the substrate to close the freeze-drying cavity and the transport channel, and a layout area is provided on the side of the cover plate away from the substrate. The layout area is used for liquid capsule arrangement, and a partial area of ​​the layout area is fluid-conducting with the liquid capsule cavity to allow the liquid reagent in the liquid capsule to circulate.

[0006] In one embodiment, the substrate is provided with a plurality of liquid capsule cavities and a plurality of freeze-drying cavities, the delivery channel includes a main channel, and a first branch channel and a second branch channel both connected to the main channel, each of the first branch channels is also correspondingly connected to each of the liquid capsule cavities, each of the second branch channels is also correspondingly connected to each of the freeze-drying cavities, and the layout area is fluidly connected to the liquid capsule cavity.

[0007] In one embodiment, the projection of the layout area on the substrate covers all the liquid capsule cavities.

[0008] In one embodiment, the microfluidic chip is provided with a spline, and the delivery channel is arranged around the spline.

[0009] In one embodiment, the first branch flow channel and the liquid capsule cavity are located in the inner circle of the main flow channel, and the second branch flow channel is located in the outer circle of the main flow channel.

[0010] The present application also provides a microfluidic chip system, which includes freeze-dried balls, liquid capsules and the microfluidic chip as described above, wherein the freeze-dried balls are arranged in the freeze-drying chamber, and the liquid capsules are arranged in the layout area.

[0011] In one embodiment, a plurality of freeze-dried balls are provided in the freeze-drying chamber.

[0012] In one embodiment, different types of freeze-dried balls are provided in the freeze-drying chamber.

[0013] In one embodiment, there are multiple liquid capsules, and the multiple liquid capsules are all arranged in the deployment area.

[0014] In one embodiment, the type of the liquid reagent contained in at least one of the liquid capsules is different from the type of the liquid reagent contained in the other liquid capsules.

[0015] In the above-mentioned microfluidic chip, the freeze-drying chamber accommodates the freeze-dried balls, and the liquid capsule is arranged in the layout area. Since the delivery channel connects the freeze-drying chamber and the liquid capsule chamber respectively, when the liquid capsule is pressed to destroy it, the liquid reagent in the liquid capsule can flow from the layout area to the liquid capsule chamber, and flow into the freeze-drying chamber through the liquid capsule chamber and the delivery channel, dissolving the freeze-dried balls and reacting with the freeze-dried reagent. The microfluidic chip is preset with freeze-dried balls and liquid capsules, so the liquid reagent can react with the freeze-dried balls by pressing the liquid capsule, which simplifies the operation of the microfluidic chip and facilitates automation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 An exploded schematic diagram of a microfluidic chip system provided in one embodiment of the present application.

[0017] Figure 2 for Figure 1 Top view of the substrate in the microfluidic chip shown.

[0018] Description of the drawings: 10, microfluidic chip system; 100, microfluidic chip; 101, spline; 110, substrate; 111, freeze-drying chamber; 112, delivery channel; 112a, main channel; 112b, first branch channel; 112c, second branch channel; 113, liquid capsule cavity; 120, cover plate; 120a, layout area; 121, liquid outlet; 200, liquid capsule. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0020] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0021] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0022] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0023] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0024] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0025] See also Figure 1 , Figure 1 An exploded schematic diagram of a microfluidic chip system in an embodiment of the present application is shown. The microfluidic chip 100 can pre-package dry reagents and wet reagents, thereby eliminating the need to introduce dry and wet reagents from the outside, simplifying the operation difficulty of the microfluidic chip 100 and facilitating the automation of the operation of the microfluidic chip 100.

[0026] See also Figure 1 and Figure 2 In one embodiment, the microfluidic chip 100 includes a substrate 110 and a cover plate 120. The substrate 110 is provided with a freeze-drying chamber 111, a delivery channel 112 and a liquid capsule chamber 113. The delivery channel 112 connects the liquid capsule chamber 113 and the freeze-drying chamber 111 respectively. The freeze-drying chamber 111 is used to accommodate freeze-dried balls. The cover plate 120 is bonded to the substrate 110 to close the freeze-drying chamber 111 and the delivery channel 112. A layout area 120a is provided on the side of the cover plate 120 away from the substrate 110. The layout area 120a is used to arrange the liquid capsule 200. In addition, a part of the layout area 120a is fluidly connected to the liquid capsule chamber 113 to allow the liquid reagent in the liquid capsule to circulate.

[0027] In the above-mentioned microfluidic chip 100, the freeze-drying chamber 111 accommodates freeze-dried balls, and the liquid capsule is arranged in the layout area 120a. Since the delivery channel 112 is connected to the freeze-drying chamber 111 and the liquid capsule chamber 113 respectively, when the liquid capsule is pressed to destroy the liquid capsule, the liquid reagent in the liquid capsule can flow from the layout area 120a to the liquid capsule chamber 113, and flow into the freeze-drying chamber 111 through the liquid capsule chamber 113 and the delivery channel 112, dissolving the freeze-dried balls and reacting with the freeze-dried reagent. The microfluidic chip 100 is pre-set with freeze-dried balls and liquid capsules, so the liquid reagent can react with the freeze-dried balls by pressing the liquid capsule, which simplifies the operation of the microfluidic chip 100 and facilitates automation.

[0028] In one embodiment, a liquid outlet hole 121 may be provided through the layout area 120a of the cover plate 120, and the liquid outlet hole 121 is used to connect the layout area 120a with the liquid capsule cavity 113. In other words, after the liquid capsule cavity 113 is squeezed, the liquid reagent therein may flow into the liquid capsule cavity 113 through the liquid outlet hole 121, and then enter the freeze-drying cavity 111 under the transportation of the transportation channel 112 to participate in the reaction.

[0029] See also Figure 2 In one embodiment, the substrate 110 is provided with a plurality of freeze-drying chambers 111 to accommodate a plurality of freeze-dried balls or a plurality of freeze-dried balls. The substrate 110 is also provided with a plurality of liquid capsule chambers 113 to receive liquid reagents in a plurality of liquid capsules. The delivery channel 112 includes a main channel 112a, and a first branch channel 112b and a second branch channel 112c both connected to the main channel 112a, and the layout area 120a is in fluid communication with the liquid capsule chamber 113. Each first branch channel 112b is also connected to each liquid capsule chamber 113, and the first branch channel 112b can conduct the liquid reagent in each liquid capsule chamber 113 to the main channel 112a. Each second branch channel 112c is also connected to each freeze-drying chamber 111, and the second branch channel 112c can conduct the liquid reagent in the main channel 112a to each freeze-drying chamber 111. Thus, the liquid capsule cavity 113 cooperates with the delivery channel 112 to deliver the liquid reagent to different freeze-drying chambers 111 to meet the reaction requirements of the microfluidic chip system 10. At this time, the flow path of the liquid reagent is: the layout area 120a, the liquid outlet 121, the liquid capsule cavity 113, the first branch channel 112b, the main channel 112a, the second branch channel 112c and the freeze-drying chamber 111.

[0030] See also Figure 1 In one embodiment, the projection of the layout area 120a on the substrate 110 covers all the liquid capsule cavities 113, so that the liquid capsules in the layout area 120a can transfer the liquid reagent to the liquid capsule cavities 113, which can simplify the structure of the microfluidic chip 100. Figure 1 As shown, the layout area 120 a may be located above each liquid capsule cavity 113 .

[0031] In one embodiment, the microfluidic chip system 10 further includes a driving device, which is connected to the microfluidic chip 100 to enable the liquid reagent in the delivery channel 112 to flow. In this way, through the driving action of the driving device, the delivery channel 110 can deliver the liquid reagent in the liquid capsule 200 to the designated freeze-drying chamber 111.

[0032] In one embodiment, the microfluidic chip 100 further includes a valve body assembly, which is disposed in the delivery channel 112 . The flow path of the liquid reagent in the delivery channel 112 can be adjusted by opening and closing the valve body assembly and switching the channel.

[0033] For example, a switch valve may be provided in the second branch flow channel 112c to control the conduction and cutoff of the second branch flow channel 112c. Thus, by opening and closing the switch valve, it is possible to control whether the liquid reagent flows into the designated freeze-drying chamber 111. Of course, the flow channel distribution and hydraulic circuit control elements on the substrate 110 may be specifically designed according to actual reaction requirements, which will not be described in detail here.

[0034] See also Figure 2 In one embodiment, the microfluidic chip 100 is provided with a spline 101, and the spline 101 is used to connect with the driving device. The delivery channel 112 is arranged around the spline 101, and when the driving device drives the microfluidic chip 100 to move through the spline 101, the liquid reagent in the delivery channel 112 is subjected to the centrifugal force and can flow along the expected trajectory.

[0035] Further, the main channel 112a is arranged around the spline 101, the first branch channel 112b and the liquid capsule cavity 113 are arranged in the inner circle of the main channel 112a, and the second branch channel 112c is arranged in the outer circle of the main channel 112a. Since the first branch channel 112b and the liquid capsule cavity 113 are arranged in the inner circle of the main channel 112a, and the first branch channel 112b is connected between the liquid capsule cavity 113 and the main channel 112a, when the driving device drives the microfluidic chip 100 to rotate through the spline 101, the liquid reagent in the liquid capsule cavity 113 can enter the main channel 112a through the first branch channel 112b. Similarly, the second branch channel 112c is located at the outer ring of the main channel 112a. Therefore, when the microfluidic chip 100 rotates, the liquid reagent in the transport channel 112 will be subjected to the centrifugal force as the microfluidic chip 100 rotates, causing the liquid reagent to flow from the main channel 112a to the outer ring direction to enter the second branch channel 112c. In other words, in this embodiment, the microfluidic chip system 10 can be driven by centrifugal force to flow liquid reagents. The spline 101 can be arranged in the central area of ​​the substrate 110 and the cover plate 120 for the output shaft of the driving device to be installed. Of course, the spline 101 can also be set to other structures that can be connected to the driving device in a transmission manner, which will not be repeated here.

[0036] Of course, the driving device is not limited to driving the liquid reagent to flow by centrifugal force. For example, the driving device can also be a liquid pump, which is connected to the delivery channel 112 to drive the liquid reagent in the delivery channel 112 to flow.

[0037] Please participate again Figure 1 In one embodiment, the liquid capsule 200 can be adhered to the cover plate 120 by gluing, and covers the corresponding liquid outlet 121. For example, the liquid capsule 200 is adhered by double-sided tape or glue.

[0038] In one embodiment, a plurality of freeze-dried balls may be provided in the freeze-drying chamber 111, that is, the shape of the freeze-drying chamber 111 is not limited to the same shape and size as the freeze-dried balls. Of course, only one freeze-dried ball may be provided in a freeze-drying chamber 111 according to requirements.

[0039] In one embodiment, different types of freeze-dried balls are provided in the freeze-drying chamber 111 to meet the reaction requirements.

[0040] The type of the liquid reagent contained in at least one of the liquid capsules 200 is different from the type of the liquid reagent contained in the other liquid capsules 200 to meet the reaction requirements.

[0041] In one embodiment, the material of the substrate 110 and the cover plate 120 may be at least one of polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS), polycarbonate (PC) and polystyrene (PS). The delivery channel 112 may be obtained by injection molding.

[0042] In one embodiment, the number of freeze-dried balls can be 2, 3, 4 or 5. The types of freeze-dried balls in the freeze-drying chamber 111 can be 2-3. The number of liquid capsules 200 can be 5, 6, 7 or 8, etc. The types of liquid reagents in the liquid capsule 200 can be 3-5. The filling volume of the liquid capsule 200 can be 800ml-1200ml.

[0043] To facilitate understanding of the microfluidic chip system 10 described in each embodiment, the assembly method of the microfluidic chip 100 is briefly described below:

[0044] Step 1, providing a substrate 110 and a cover plate 120, wherein the substrate 110 is provided with a delivery channel 112 and a plurality of freeze-drying chambers 111, and the cover plate 120 is provided with a liquid outlet 121 and a layout area 120a.

[0045] Step 2, loading the freeze-dried balls of different reagent components into the freeze-drying chamber 111 respectively.

[0046] Step 3: Bond the substrate 110 to the cover plate 120. Thus, the delivery channel 112 and the chip cavity can be sealed.

[0047] Step 4: attach the liquid capsule 200 to the layout area 120 a to communicate with the liquid outlet 121 .

[0048] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0049] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A microfluidic chip, characterized in that: The microfluidic chip comprises: A substrate, wherein the substrate is provided with a delivery channel, a liquid capsule cavity and a freeze-drying cavity, wherein the delivery channel is connected to the liquid capsule cavity and the freeze-drying cavity respectively, and the freeze-drying cavity is used to accommodate freeze-dried balls; and A cover plate is bonded to the base plate to close the freeze-drying chamber and the delivery channel. A layout area is provided on the side of the cover plate facing away from the base plate. The layout area is used for arranging liquid capsules, and a part of the layout area is fluidly connected to the liquid capsule cavity to allow the liquid reagent in the liquid capsule to circulate.

2. The microfluidic chip according to claim 1, characterized in that: The substrate is provided with a plurality of liquid capsule cavities and a plurality of freeze-drying cavities, the delivery channel comprises a main channel, and a first branch channel and a second branch channel both connected to the main channel, each of the first branch channels is also correspondingly connected to each of the liquid capsule cavities, each of the second branch channels is also correspondingly connected to each of the freeze-drying cavities, and the layout area is in fluid communication with the liquid capsule cavity.

3. The microfluidic chip according to claim 2, characterized in that: The projection of the layout area on the substrate covers all the liquid capsule cavities.

4. The microfluidic chip according to claim 2, characterized in that: The microfluidic chip is provided with a spline, and the delivery channel is arranged around the spline.

5. The microfluidic chip according to claim 4, characterized in that: The first branch flow channel and the liquid capsule cavity are located in the inner circle of the main flow channel, and the second branch flow channel is located in the outer circle of the main flow channel.

6. A microfluidic chip system, characterized in that: The microfluidic chip system comprises freeze-dried balls, liquid capsules and the microfluidic chip according to any one of claims 1 to 5, wherein the freeze-dried balls are arranged in the freeze-drying chamber, and the liquid capsules are arranged in the layout area.

7. The microfluidic chip system according to claim 6, characterized in that: A plurality of freeze-dried balls are arranged in the freeze-drying chamber.

8. The microfluidic chip system according to claim 6, characterized in that: Different types of freeze-dried balls are arranged in the freeze-drying chamber.

9. The microfluidic chip system according to claim 6, characterized in that: There are multiple liquid capsules, and the multiple liquid capsules are all arranged in the layout area.

10. The microfluidic chip system according to claim 6, characterized in that: The type of the liquid reagent contained in at least one of the liquid capsules is different from the type of the liquid reagent contained in the other liquid capsules.

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