Microorganism detection board card

Abstract

The utility model discloses a microbiological detection board card, which is characterized by comprising a glass slide, the cover glass is arranged opposite to the glass slide in the thickness direction of the glass slide; the supporting layer is provided with one or more hollow patterns, the supporting layer is located between the glass slide and the cover glass, and the supporting layer, the glass slide and the cover glass are connected to form one or more independent cavities; the chamber includes an observation chamber. Seepage is reduced through fragmented synchronous bonding, and convenience of detection of different microorganisms in different environments is improved.

Description

Technical Field

[0001] The utility model relates to the field of microbial detection, in particular to a microbial detection board card. Background Art

[0002] With the development of science and technology and the increasing awareness of human health and the environment, the microbial testing industry is developing rapidly. Microbial testing is the field of detection and identification of microorganisms, and is widely used in many fields such as medicine, food, environmental protection, and agriculture. In the future, the microbial testing industry will face more opportunities and challenges. With the development of the global economy and the improvement of people's awareness of health and the environment, the demand for the microbial testing market will continue to grow. At the same time, with the advancement of technology, the methods and means of microbial testing will continue to improve and perfect. In the medical field, microbial testing will play a more important role in disease diagnosis, treatment, and prevention.

[0003] With the continuous development of medical technology, microbial testing will become more accurate and efficient, providing more reliable support for the medical field. In the fields of food and environmental protection, microbial testing will also play a more important role. As people's requirements for food and environmental safety continue to increase, microbial testing will provide more accurate and efficient methods and means for testing in the fields of food and environmental protection. In the agricultural field, microbial testing will provide more scientific and sustainable support for agricultural production. Through microbial testing, we can better understand the microbial communities in soil, water sources and crops, and provide more accurate and efficient support for agricultural production. In general, the microbial testing industry will face broader development prospects in the future.

[0004] Therefore, there is a need in the art for a sufficiently convenient microbial detection board that can meet the needs of different microbial detection in complex environments. Utility Model Content

[0005] The purpose of the utility model is to provide a microbial detection board card, which reduces the leakage through the slice-type synchronous bonding and improves the convenience of different microbial detection in different environments. The present invention relates to the intersection of biological and medical detection and micro-nano processing, and in particular to the detection of sperm and some cells, bacteria, fungi and other microbial screening instruments.

[0006] According to one aspect of the utility model, a microorganism detection board is provided, comprising: a glass slide; a cover glass, wherein the cover glass is placed opposite to the glass slide in the thickness direction of the glass slide; a support layer, wherein the support layer has one or more hollow patterns, the support layer is located between the glass slide and the cover glass, and is connected to the glass slide and the cover glass to form one or more independent chambers; the chamber includes an observation chamber.

[0007] In some embodiments, the observation chamber comprises an inlet and an outlet.

[0008] In some embodiments, the cover glass is located above the observation chamber in the thickness direction of the slide.

[0009] In some embodiments, the observation chamber includes one or more anchor points connected to the slide and the cover glass.

[0010] In some embodiments, the chamber includes a permeate chamber located at an edge of the observation chamber.

[0011] In some embodiments, the liquid permeation chamber located between two adjacent observation chambers includes a capillary area and a liquid storage area.

[0012] In some embodiments, the capillary region and the liquid storage region are located downstream of the liquid permeation chamber.

[0013] In some embodiments, the reservoir region is located downstream of the capillary region.

[0014] In some embodiments, the cover glass is the same or different type of lens.

[0015] In some embodiments, the cover glasses are made of the same or different materials.

[0016] In some embodiments, a filter is included, the filter is placed parallel to the cover glass, the filter is connected to the support layer, and the filter, the support layer and the slide glass form one or more independent chambers.

[0017] In some embodiments, the filters are made of the same or different materials.

[0018] Compared with the prior art, the microbial detection board provided by the utility model reduces the leakage through the slice-type synchronous bonding, and can bond different cover glasses according to different observation requirements to be applied to control experiments in scientific research or medicine. At the same time, different filters can be bonded to increase the activity of various microorganisms and reduce the light temperature to achieve better observation effects.

[0019] These and other features and aspects of the present invention will become more apparent from the following detailed description made with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings of the embodiments are briefly introduced below. Obviously, the drawings described below only relate to some embodiments of the present application, but are not intended to limit the present application, wherein:

[0021] Figure 1 A top view bar diagram of a microorganism detection board according to the first embodiment of the utility model is shown.

[0022] Figure 2 A three-dimensional diagram of a microorganism detection board card according to the first embodiment of the utility model is shown.

[0023] Figure 3 A front view of a microorganism detection board according to the first embodiment of the utility model is shown.

[0024] Figure 4 A front cross-sectional view of a microorganism detection board according to the first embodiment of the utility model is shown.

[0025] Figure 5 A side view of a microorganism detection board according to an embodiment of the utility model is shown.

[0026] Figure 6 A side sectional view of a microorganism detection board according to an embodiment of the utility model is shown.

[0027] Figure 7 A top view of a microorganism detection board without a cover glass and a filter according to an embodiment of the utility model is shown.

[0028] Figure 8 A top view line diagram of a microbial detection board card with cover glasses or filters bonded to multiple observation chambers according to the second embodiment of the present utility model is shown. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0030] In the drawings, the shapes and dimensions may be exaggerated for clarity, and the same reference numerals will be used throughout to designate the same or like components.

[0031] Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by persons of ordinary skill in the field to which this application belongs. The words "first", "second" and similar words used in the patent application specification and claims of this application do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one", "one" or "the" do not indicate a quantitative limitation, but indicate the existence of at least one. Words such as "include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" include the elements or objects listed after "include" or "comprise" and their equivalents, and do not exclude other elements or objects. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0032] In the following description, words such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are defined relative to the structure shown in the drawings. In particular, "height" is equivalent to the dimension from top to bottom, "width" is equivalent to the dimension from left to right, and "depth" is equivalent to the dimension from front to back. They are relative concepts and may change accordingly according to their different positions and different usage states. Therefore, these or other directions should not be interpreted as restrictive terms.

[0033] Terms related to attachment, coupling, and the like (eg, "connected" and "attached") refer to either a fixed or attached relationship between structures, either directly or indirectly, and either movable or rigid attachments or relationships, unless expressly stated otherwise, of the structures to one another through intermediate structures.

[0034] The specific implementation of the utility model is further described in detail below in conjunction with the drawings and embodiments.

[0035] Figure 1 It is a three-dimensional schematic diagram of the first embodiment of the microorganism detection board card.

[0036] like Figure 1 As shown, the microbial detection board includes a glass slide 7, which is used to support and hold the sample and can be a transparent glass sheet or a plastic sheet. The surface of the glass slide can be processed, including polishing, coating or other treatments to improve its optical properties or sample adhesion.

[0037] The microorganism detection board includes a cover glass 6, which is placed opposite to the slide glass 7 in the thickness direction. Different cover glasses can be bonded according to different observation requirements. The cover glass is made of transparent materials, such as glass or plastic. Preferably, the surface of the cover glass is modified and the cover glass is formed into a concave lens or a convex lens by etching to achieve the effect of magnification or reduction. Preferably, the material of the cover glass is changed by bonding cover glasses of different materials or performing different surface modifications on the cover glasses of the same material to achieve different effects, which can be applied to control experiments in scientific research or medicine.

[0038] The microorganism detection board includes a filter 8, which is placed parallel to the cover glass. The filter is used to adjust or change the optical properties of the sample in the optical device. The filter can be made of transparent materials, such as glass or plastic, and has specific optical properties to filter or adjust light in a specific wavelength range. In the case of self-fluorescence or mixed in fluorescent reagents, it is necessary to observe under a specific spectrum, and the observation effect can be achieved by bonding different filters. Preferably, the filter can be designed to absorb, transmit or reflect light of a specific wavelength, thereby changing the optical effect of the sample during microscopic observation or analysis. Preferably, the filter can be used to reduce the light temperature and increase the activity of microorganisms. Since some microorganisms have reduced activity or even death under specific light sources, some harmful light sources can be filtered out by unused filters.

[0039] The microorganism detection board includes a support layer 5, which has one or more hollow patterns, and is combined with a cover glass 6, a slide glass 7 and / or a filter 8 to form one or more independent chambers. The normal height of the chamber is 1-300um, and the optimal chamber height can be selected according to customer needs. The support layer 5 can be made of ink, photoresist, gel, adhesive, etc., among which polyimide (PI), polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), acrylic photoresist (HTG910) and color ink are preferably obtained by photolithography process, exposure and development.

[0040] Figure 2 A three-dimensional diagram of a microorganism detection board card according to the first embodiment of the utility model is shown. Figure 3 A front view of a microorganism detection board according to the first embodiment of the utility model is shown. Figure 4 A front cross-sectional view of a microorganism detection board according to the first embodiment of the utility model is shown.

[0041] Combination Figures 2 to 4It can be seen that the multiple chambers formed by the glass slide 7, the cover glass 6, the filter 8 and the support layer 5 include an observation chamber 3 for observing the state of microorganisms. In the thickness direction of the glass slide, a cover glass or a filter can be bonded above the observation chamber 3 to achieve different experimental purposes. Each observation chamber 3 includes an inlet 1 and an outlet 2, and the shapes of the inlet 1 and the outlet 2 can be two large and small boxes, or can have other different shapes.

[0042] The multiple chambers formed by the glass slide 7, the cover glass 6, the filter 8 and the support layer 5 include a liquid seepage chamber 4, which is located between and / or at the edge of the observation chamber. Preferably, the upper half of the liquid seepage chamber 4 can be treated with hydrophobicity, and the lower half can be treated with hydrophilicity. Such treatment can better make the liquid flow downward, and can completely prevent the sample from seeping to the outside and the adjacent chamber. Through osmotic diffusion, the microorganisms are inhaled from the sample inlet 1 and diffused to the entire observation chamber 3, and the excess liquid flows out from the sample outlet 2 and the liquid seepage chamber 4.

[0043] The seepage cavity between the observation chambers includes a capillary area 9 and a liquid storage area 10, and the capillary area 9 and the liquid storage area 10 are located downstream of the seepage cavity, and the liquid storage area 10 is located downstream of the capillary area 9. The liquid flows downward from the seepage cavity 4 through the capillary area 9, and the liquid quickly enters the liquid storage area 10 through the capillary action. The liquid storage area 10 can be of any shape. The liquid storage area 10 can be made by techniques such as photolithography, silk screen printing, and nanoimprinting (NIL), preferably photolithography, which has higher precision and better effect. Preferably, in the thickness direction of the slide, the surface of the cover glass and / or filter facing the slide above the liquid storage area includes a groove, which can store more liquid. Further, the depth of the liquid storage area can be deepened, and the position of the corresponding liquid storage area on the surface of the cover glass and the filter facing the slide is etched by glass etching and other methods. Glass etching is divided into wet etching and dry etching. In the case of good precision and low price, wet etching is preferred. Preferably, microspheres may be used to perform surface modification in the liquid storage area to adsorb specific substances.

[0044] Figure 5 A side view of a microorganism detection board according to an embodiment of the utility model is shown. Figure 6 A side sectional view of a microorganism detection board according to an embodiment of the utility model is shown. Figure 7 A top view of a microorganism detection board without a cover glass and a filter according to an embodiment of the utility model is shown.

[0045] Combination Figures 3 to 7The entire glass slide 7 and the support layer 5 are bonded together with the cover glass 6 and the filter 8 through a slice-type synchronous bonding process, wherein the observation chamber 3 includes one or more anchor points 11, and the anchor points 11 are connected to the glass slide, the filter, and the cover glass. Through the arrangement and support of the anchor points 11, the chamber can be supported from collapsing and the liquid inside can be prevented from turbulent, thereby increasing the injection speed and improving the inspection efficiency.

[0046] Figure 8 A top view line diagram of a microbial detection board card with cover glasses bonded to multiple observation chambers according to the second embodiment of the present utility model is shown.

[0047] Combination Figure 8 , the coverslips bonded to the multiple observation chambers can all be flat mirrors, concave lenses or convex lenses, can be made of different materials, or can be coated with different layers. When the microorganisms in the observation chambers are the same, they can be used for control experiments of microbial detection control variables in scientific research or medical fields. Further, the filters bonded to the multiple observation chambers can all be optical filters, and the optical filters can be made of materials, layers, and materials with different optical effects. When the microorganisms in the observation chambers are the same, they can be used for control experiments of microbial detection control variables in scientific research or medical fields.

[0048] The embodiments of the present invention are described above, and these embodiments do not describe all the details in detail, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and changes can be made based on the above description. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can make good use of the present invention and the modifications based on the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A microbial detection card, characterized in that: include: Glass slides; A cover glass, wherein the cover glass is placed opposite to the slide glass in the thickness direction of the slide glass; A support layer, the support layer having one or more hollow patterns, the support layer being located between the glass slide and the cover glass, and connected with the glass slide and the cover glass to form one or more independent chambers; The chamber includes an observation chamber.

2. The microbial detection board according to claim 1, characterized in that: The observation chamber comprises an inlet and an outlet.

3. The microbial detection board according to claim 1, characterized in that: In the thickness direction of the slide glass, the cover glass is located above the observation chamber.

4. The microbial detection board according to claim 1, characterized in that: The observation chamber includes one or more anchor points, which are connected to the slide and the cover glass.

5. The microbial detection board according to claim 1, characterized in that: The chamber includes a seepage chamber, and the seepage chamber is located at the edge of the observation chamber.

6. The microorganism detection board according to claim 5, characterized in that: The liquid seepage cavity located between two adjacent observation chambers includes a capillary area and a liquid storage area.

7. The microorganism detection board according to claim 6, characterized in that: The capillary region and the liquid storage region are located downstream of the liquid permeation chamber.

8. The microorganism detection board according to claim 7, characterized in that: The liquid storage area is located downstream of the capillary area.

9. The microorganism detection board according to claim 1, characterized in that: The cover glass is the same or different type of lens.

10. The microorganism detection board according to claim 1, characterized in that: The cover glasses are made of the same or different materials.

11. The microorganism detection board according to claim 1, characterized in that: It comprises a filter, which is placed parallel to the cover glass and connected to the support layer. The filter, the support layer and the slide glass form one or more independent chambers.

12. The microorganism detection board according to claim 11, characterized in that: The filters are made of the same or different materials.

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