Automatic staining device for peripheral blood smear and bone marrow smear

Abstract

The utility model discloses an automatic dyeing device for a peripheral blood smear and a bone marrow smear. Comprising a shell (1), a cover body (2), a liquid storage box mounted in the shell (1), a control panel (3), a display control panel (4), a dyeing cavity for placing a smear (6), a plurality of dyeing tanks (5), a plurality of steam nozzles (21) formed in the top wall of the cover body, and a pipeline system for supplying and discharging liquid to each group of dyeing tanks (5), each group of pipeline system comprises a set of dye liquor supply pipeline, a set of water-gas co-pipeline and a set of liquor discharge pipeline; the device has the functions of automatic dye liquor adding, pneumatic uniform mixing, flushing and drying, full-automatic continuous operation can be achieved, and efficiency is improved. The device can be controlled in groups according to needs, can meet the requirement for simultaneous dyeing of a single group and multiple groups, saves dye liquor, is economical and practical, improves efficiency and saves cost.

Description

Technical Field

[0001] The utility model relates to the technical field of equipment related to blood cell morphology inspection, in particular to an automatic staining device for peripheral blood smear and bone marrow smear. Background Art

[0002] Blood cell morphology is a commonly used inspection method in hematology, especially in routine blood tests. At present, with the introduction of blood cell analyzers, blood smear staining is gradually moving towards automation. However, manual staining is still the main method used in hospitals at all levels. Although manual staining can achieve better staining effects, it also has some disadvantages and shortcomings. The main reason is that most of the current blood cell analyzers have complex functions, large equipment and high prices, high learning costs for learning and use, and high maintenance and repair management costs. In a single inspection, there is a lack of a special equipment with a compact structure, low cost, simple and efficient operation. For example, in the field of automatic staining of peripheral blood smears and bone marrow smears, manual staining is still the main method in daily work, but manual staining is inefficient and time-consuming. Compared with automated instruments, manual staining requires the operator to drip the dye on the slide step by step, which is cumbersome and time-consuming, greatly affected by human factors, and cannot quickly complete the processing of a large number of blood samples. This limits the efficiency and rapidity of blood cell morphology examination. Secondly, manual staining technology requires high experience and skills of the operator. During the dyeing process, the uniformity of dye application and the dyeing time affect the accuracy of the dyeing results. However, due to the different experience and skill levels of operators, the quality of dyeing may vary, resulting in unstable reliability of the results. Secondly, for individual hospitals or clinical laboratories, the number of smears that need to be checked each time is usually uncertain, ranging from 3-6 pieces for a small number to more than ten pieces for a large number. Current automatic detectors usually require batch testing, which will result in waste of dye solution.

[0003] In summary, although manual staining is still the main means of blood smear staining, its disadvantages and shortcomings such as low efficiency, time-consuming and labor-intensive, difficult operation, and inconvenient use limit its application in clinical practice. The existing automatic detectors are expensive, complex in function, and large in size, and cannot perform economical and efficient staining for small batches of smears. Based on this, how to design a small-structure, low-manufacturing-cost, and easy-to-use automatic staining device to perform economical and efficient automatic staining for small batches of smears is a current technical gap. Summary of the invention

[0004] In order to solve the above-mentioned deficiencies and defects in the prior art, the inventor has developed and designed a peripheral blood smear and bone marrow smear automatic staining device, which has a compact and reasonable structure, low manufacturing cost, and can be applied to small batches of smear automatic staining operations. It has the functions of timed addition of dye, timed dilution, automatic drainage, cleaning and drying, etc. It is economical and practical, has low operating difficulty, and the system is easy to use and stable. The utility model is mainly achieved as follows:

[0005] A peripheral blood smear and bone marrow smear automatic staining device comprises a shell, a cover, a liquid storage box installed inside the shell, a control board, a display control panel, and a staining cavity for placing smears, wherein the device has a plurality of staining slots, which are divided into a group with each horizontal row or column, and a plurality of groups are placed side by side in the staining cavity, and each smear is placed in a staining slot, and at least one liquid material adding hole is opened on the inner wall of the upper edge of each staining slot, and a drainage hole is arranged on the bottom of the staining slot; there are a plurality of water vapor nozzles, which are arranged on the inner top wall of the cover, and the number and position of the distribution are adapted to the number and position of the staining slots, so that when the cover covers the shell, the staining cavity can be covered from above, and Each water-gas nozzle corresponds to a dyeing tank, which is used to spray clean water to clean the dyeing tank and spray air to dry the dyeing tank; the pipeline system is used to supply and drain liquid to each group of dyeing tanks, and each group of pipeline systems includes a set of dye liquid supply pipelines, a set of water-gas common pipelines, and a set of drainage pipelines; the dye liquid supply pipeline provides the dyeing tanks of each group with liquid A and liquid B; the drainage pipeline provides the dyeing tanks of each group with a channel for discharging dye liquid and suction power; the water-gas common pipeline provides clean water for each group of dyeing tanks, as well as spray flushing and air flow drying; the control board connects and controls the pipeline system and the display and control panel, and the power supply module is connected to the control board, the pipeline system and the display and control panel and is powered by wires.

[0006] The working principle of the utility model is introduced as follows: the shell and the cover of the utility model are adapted to each other, and the cover can be opened and lowered. The purpose of the cover is not only to cover the dyeing tank, but also to provide a channel hole for water vapor injection, and the A dye and B dye are added from the liquid material adding hole on each dyeing tank. The liquid material adding hole is added through the dye liquid supply pipeline. Specifically, the power is provided by a micro liquid supply pump to pump the dye in the liquid storage box into the pipeline and finally flow into the dyeing tank; and the distilled water added during cleaning is discharged through the water and gas co-pipe in the cover. After the operation is completed, when it is necessary to drain the liquid, negative pressure suction is achieved through the drainage pipeline, and the dye in each dye tank is sucked out from the drainage hole, and recovered and processed uniformly, and then the cleaning program is started. The device automatically sprays clean water into the dye tank again through the water-gas co-pipeline, and even cooperates with intermittent spraying of water-gas mixture to clean and rinse the dye tank, suck out the water, and then concentrate on aeration. The airflow is blown into the dye tank to dry the dye tank for secondary use. During actual use, the dye can be pumped in advance in the preset program before use to discharge the air in the tube; the excess dye A and dye B are sucked away from the drainage hole, and the drainage pump at the end of the drainage tube remains locked, forming a sealed space in the drainage tube, and the aperture of the drainage hole is relatively small, so the dye does not automatically flow into the drainage hole after entering; after the preparation program is completed, the operator manually puts in the smear to be dyed, closes the cover, ensures that there is enough dye, diluent, and clean water in the dye box, and inputs the start designation through the display control panel, the micro-liquid supply pump of dye A is started in advance and continues to run for a predetermined time, so that the running time is consistent with the efficiency of the micro-liquid supply pump. , the thickness and length of the pipeline are adapted to achieve that the timed pumped-in dye A can simultaneously enter a specific amount of dye A in each group of dye tanks to soak the smear, and the timer counts at the same time. When dye B needs to be added, the corresponding micro-liquid supply pump in the B dye box is started to add a specific amount of dye B to each group of dye tanks to achieve mixing of AB dyes. At this time, the control panel can control the intermittent start of the suction pump, and the three-way switching valve pumps the gas inhaled from the air inlet into each water vapor nozzle of the first-level water-gas common pipe to the second-level water-gas common pipe, forming a number of intermittent airflows, and intermittently blowing air from both ends of the dye tank to generate air push on the dye, thereby achieving the dye mixing function. After the mixing and blowing process is completed, after a specific period of time, the three-way switching valve is switched, the air inlet is closed, the water pipeline is opened, the suction pump is started again, and pure water is pumped into the water-gas common pipeline to form a flushing process. At the same time, the drainage pump on the drainage pipeline starts suction, and the drainage pipeline forms a negative pressure and starts suction, so that the dye in the dye tank is sucked into the drainage pipeline through the drainage hole to realize waste liquid discharge, and the dye tank is rinsed with clean water until it is clean and then stopped. After the flushing waste water is discharged, the three-way switching valve is switched again, the water pipeline is closed, the air inlet is opened, and air is injected into the water-gas common pipeline to realize continuous airflow blowing inside the dye tank for drying or drying. After completion, the cover can be manually opened to take out the dyed smear.

[0007] Compared with the prior art, the utility model has the following technical advantages:

[0008] (1) The automatic staining device for peripheral blood smears and bone marrow smears has been realized, and has a liquid addition control function for automatically adding double dye solutions at a fixed time and quantity, and an automatic pneumatic mixing function, so that the AB liquid can be mixed better and faster, and has an automatic rinsing and drying function, realizing fully automatic integrated continuous operation, which greatly saves manpower, improves efficiency, ensures the accuracy of dye addition amount and time, and further ensures the staining quality.

[0009] (2) It realizes group control, which can realize the smear dyeing operation of a single group of small batches, and can also realize the simultaneous dyeing operation of multiple groups. Whether it is a small batch or a large batch, it can meet the operation requirements and can realize independent control of a single group without causing waste of dye solution. It is economical, practical and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 One of the three-dimensional diagrams of an automatic staining device for peripheral blood smears and bone marrow smears;

[0011] Figure 2 The second is a stereoscopic diagram of an automatic staining device for peripheral blood smear and bone marrow smear;

[0012] Figure 3 It is a stereoscopic diagram of the internal structure of an automatic staining device for peripheral blood smear and bone marrow smear;

[0013] Figure 4 A schematic diagram of the installation of a liquid box of an automatic staining device for peripheral blood smear and bone marrow smear;

[0014] Figure 5 It is a three-dimensional diagram of the structure of the dye tank;

[0015] Figure 6 It is the structural front view of the dye tank;

[0016] Figure 7 This is a schematic diagram of the pipeline structure of dye solution A of the utility model;

[0017] Figure 8 This is a schematic diagram of the pipeline structure of the B dye solution of the utility model;

[0018] Fig. 9 This is a schematic diagram of the structure of the liquid discharge pipeline of the utility model;

[0019] Fig.10 This is a schematic diagram of the water-gas co-pipeline structure of the utility model;

[0020] Fig.11 This is a schematic diagram of the installation structure of the water-gas co-pipeline of the utility model;

[0021] Fig.12 It is a front view of the internal structure of an automatic staining device for peripheral blood smear and bone marrow smear;

[0022] Fig.13 This is a three-dimensional diagram of the piping system structure of a single set of dyeing tanks of the utility model;

[0023] Fig.14 This is a three-dimensional diagram of the piping system structure of the entire dyeing tank of the utility model;

[0024] in:

[0025] 1—Shell,

[0026] 2—cover, 21—water and gas nozzle, 22—water pipe, 24—three-way switching valve, 25—air inlet, 26—suction pump, 27—primary water and gas co-pipe, 28—secondary water and gas co-pipe,

[0027] 3—Control panel,

[0028] 4—Display and control panel,

[0029] 5—dyeing tank, 51—drainage hole, 52—primary liquid supply pipe, 53—micro liquid supply pump, 54—N pass, 55—secondary liquid supply pipe, 56—third-level drainage pipe, 57—secondary drainage pipe, 58—primary drainage pipe, 59—drainage pump, 501—A liquid storage box, 502—B liquid storage box, 503—distilled water storage box, 504—support bar, 505—sealing gasket, 506—A dye addition hole, 507—B dye addition hole,

[0030] 6—Smear. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model is further described in detail below in combination with specific implementation methods and with reference to the accompanying drawings. It should be understood that these descriptions are only exemplary and are not intended to limit the scope of the utility model. In addition, in the following description, the description of well-known structures and technologies is omitted to avoid unnecessary confusion of the concept of the utility model.

[0032] Embodiment 1: An automatic staining device for peripheral blood smear and bone marrow smear, comprising a shell 1, a cover 2, a liquid storage box installed inside the shell 1, a control board 3, a display control panel 4, and a staining chamber for placing a smear 6. When the cover 2 is opened, the top of the staining chamber is exposed, which is convenient for taking out or putting in the smear 6. There are three liquid storage boxes in this embodiment, a larger one is used to load distilled water or pure water, and two smaller ones are used to load liquid A and liquid B respectively. The volume can be designed to be equal, or the volume of liquid B can be larger than that of liquid A. The specific amount can be determined according to the ratio of liquid A and liquid B. The liquid storage box is usually made of transparent material and inserted from both sides of the shell 1 in a drawer-like structure; the box body is provided with a handle and other structural designs for easy operation, and small wheels can be installed at the bottom of the four corners of the shell 1 for easy movement. The display and control panel 4 involved in the present embodiment is a display screen, or a panel with touch or button input, which is used to operate the operating program of the operating device. For example, a single-group dyeing mode or a full-group dyeing mode can be selected, the dyeing liquid control ratio of liquid A and liquid B can be set, the dyeing liquid addition interval of liquid A and liquid B can be set, etc. The display and control panel 4 is also used to reflect the current operating mode, remaining time, etc.

[0033] There are several dyeing tanks 5, which are divided into a group with each horizontal row or column. Multiple groups are placed side by side in the dyeing chamber, and each smear 6 is placed in a dyeing tank 5. At least one liquid material addition hole is opened on the inner wall of the upper edge of each dyeing tank 5, and a drainage hole 51 is set on the bottom of the dyeing tank 5; in this embodiment, each group is six dyeing tanks 5 arranged side by side, and there are three groups in total. When the number of smears 6 to be dyed is less than or equal to 6 pieces, they can be placed in each dyeing tank 5 of the same group, covered with the cover body 2, and the single group dyeing mode of the corresponding group is selected; if there are more than 6 pieces, two or three groups can be selected as needed to proceed simultaneously. The liquid material addition hole is the entrance for the dye to enter the dyeing tank 5, and each dyeing tank 5 is set accordingly; the drainage hole 51 is located on the bottom of the tank at the lowest point, and is used for the entrance of the outflow channel when draining. The liquid material addition hole and the drainage hole 51 are both connected to the pipeline system.

[0034] There are a plurality of water vapor nozzles 21, which are arranged on the inner top wall of the cover body 2. The number and position of the distribution are adapted to the number and position of the dyeing slots 5, so that when the cover body 2 covers the shell body 1, the dyeing chamber can be covered from above, and each water vapor nozzle 21 corresponds to a dyeing slot 5, which is used to spray clean water on the dyeing slot 5 for cleaning and spray air to dry the dyeing slot 5; that is, the cover body 2 also serves as an important pipeline system function, providing important nozzles for clean water flushing and air flow blowing for each dyeing slot 5. When cleaning is required, the nozzle sprays water, and when drying is required, the nozzle sprays high-speed airflow.

[0035] A pipeline system is used for supplying and draining liquid to each group of dyeing tanks 5, and each group of pipeline systems includes a set of dye liquid supply pipelines, a set of water and gas co-pipe pipelines, and a set of drainage pipelines;

[0036] The dye liquid supply pipeline provides the dyeing tank 5 of each group with the inlet of liquid A and liquid B;

[0037] The drainage pipeline provides a channel for discharging dye liquid and a suction power for the dyeing tank 5 of each group;

[0038] The water-air co-pipeline provides clean water to each group of dyeing tanks 5, as well as spraying and flushing and airflow drying;

[0039] The control panel 3 is connected to and controls the pipeline system and the display control panel 4, and the power supply module is connected to the control panel 3, the pipeline system and the display control panel 4 and is powered by wires. The power supply module can be connected by a built-in battery or an external plug-in wire. The control panel 3 is used to execute a specific control circuit program, which belongs to the prior art. It issues control instructions based on specified signals, and is used to provide instructions to control the addition time, duration, and stop instructions of liquid A and liquid B, start the drainage pump 59 instruction, control the drainage pump 59 running time, and control the time and jet time of spraying clean water in the water-gas co-pipeline.

[0040] Preferably, the dye liquid supply pipeline includes: one end of the primary liquid supply pipe 52 is connected to a micro liquid supply pump 53 in the liquid storage box, the other end of the primary liquid supply pipe 52 is connected to the N-through piece 54, and one end of a plurality of equal-length secondary liquid supply pipes 55 are respectively connected through the N-through piece 54, and the other end of the secondary liquid supply pipe 55 is connected to the liquid material adding hole of each dyeing tank 5; the liquid storage box includes two, which are used to pack dye A and dye B, each liquid storage box has a plurality of micro liquid supply pumps 53 built in, and each micro liquid supply pump 53 corresponds to a set of dye liquid supply pipelines for liquid supply; the dyeing tanks 5 are arranged in three groups side by side, and each group is six dyeing tanks. The tanks 5 are vertically arranged in parallel; the liquid storage boxes include a liquid A storage box 501, a liquid B storage box 502, and a distilled water storage box 503, and are installed in the lower part of the housing 1 in a horizontal insertion installation mode, wherein the liquid A storage box 501 and the liquid B storage box 502 are inserted from one side of the housing 1, and the distilled water storage box 503 is inserted from the other side; three micro liquid supply pumps 53 are respectively arranged in the liquid A storage box 501 and the liquid B storage box 502, and the micro liquid supply pumps 53 are installed on the end of the primary liquid supply pipe 52; the three groups of dyeing tanks 5 each correspond to a set of water and gas co-pipeline, a set of dye liquid supply pipeline, and a set of drainage pipeline. In this embodiment, three micro liquid supply pumps 53 are respectively arranged in the liquid storage boxes of the A and B dye liquids, and each micro liquid supply pump 53 corresponds to a group of dyeing tanks 5. When one of the micro-liquid supply pumps 53 receives the start signal from the control board 3, it starts running immediately, and the A dye solution is pumped into the primary liquid supply pipe 52, and then flows into the six secondary liquid supply pipes 55 after passing through the N-through piece 54, and is connected to the A dye solution addition hole 506 in each dyeing tank 5 through the secondary liquid supply pipe 55, and flows out from the hole and enters the dyeing tank 5. The amount of A dye solution required is controlled by the running time and its efficiency of the micro-liquid supply pump 53, and is finally reflected in the running time to achieve quantitative addition of A dye solution. Similarly, the B dye solution storage box also has the same pipeline design and micro-liquid supply pump 53, and also adds B dye solution to the dyeing tank 5 through the primary liquid supply pipe 52 and the secondary liquid supply pipe 55.

[0041] The water-gas co-pipeline comprises: one end of a water body pipe 22 is connected to the bottom of the water box, and the other end is connected to a first interface of a three-way switching valve 24, the second interface of the three-way switching valve 24 is an air inlet 25, the second interface of the three-way switching valve 24 is connected to a suction pump 26, the outlet end of the suction pump 26 is connected to a primary water-gas co-pipe 27, the upper branch of the primary water-gas co-pipe 27 is connected to one end of a plurality of secondary water-gas co-pipes 28, and the other end of the secondary water-gas co-pipe 28 is connected to each of the water-gas nozzles 21; each set of water-gas co-pipes corresponds to a group of dyeing tanks 5;

[0042] The drainage pipeline includes: one end of a plurality of three-level drainage pipes 56 is connected to the drainage hole 51 at the bottom of each dyeing tank 5, and the other end is connected to the second-level drainage pipe 57; the end of the second-level drainage pipe 57 is connected to the first-level drainage pipe 58, and the end of the first-level drainage pipe 58 is connected to the drainage pump 59, and the drainage pump 59 provides negative pressure in the pipeline to suck and discharge waste liquid and waste water. When it is necessary to discharge the dye liquid, the control board 3 sends a start designation to the drainage pump 59, and the drainage pump 59 starts to suck and discharge the first-level drainage pipe 58 outward, forming a negative pressure in the pipe, thereby driving the entire second-level drainage pipe 57 and the third-level drainage pipe 56 to be in a negative pressure state, thereby realizing the negative pressure of the drainage hole 51, and sucking and discharging the dye liquid in the dyeing tank 5, and the other end of the drainage pump 59 can be externally connected to a sewage pipe, or a special barrel for waste liquid collection.

[0043] Preferably, the bottom of the dyeing tank 5 is an inclined tank with four sides gradually inclined toward the center. A slot is provided at the top of one side of the dyeing tank 5, which is convenient for taking or placing the smear 6 from one end. Two supporting strips 504 are horizontally provided in the middle of the dyeing tank 5 for horizontally supporting the smear 6; a drainage solenoid valve is installed on the primary drainage pipe 58, and the drainage solenoid valve is connected to the control board 3. The inclined tank design can ensure that the dye can cover the drainage hole 51, and the drainage hole 51 is arranged on the bottom surface of the inclined tank to improve the completion amount of drainage. The design of the supporting strip 504 is to enable the smear 6 to be placed horizontally and stably. The setting of the drainage solenoid valve is to ensure that when the drainage is not actively sucked, the dye eventually enters the drainage pipe, the primary and secondary drainage pipes 57, causing the problem of insufficient and inaccurate dye addition. Therefore, the drainage solenoid valve is provided. At this time, even if the dye enters the primary drainage pipe 58, it will not flow into the secondary and tertiary drainage pipes 56, ensuring the accuracy of the amount of dye in the dye tank. When draining, just control the drain solenoid valve to open at the same time.

[0044] Preferably, the three-way switching valve 24, the suction pump 26, the primary water-gas common pipe 27 and the secondary water-gas common pipe 28 are all installed in the cover body 2, and a breathable mesh is provided on the top of the cover body 2 above the air inlet 25 of the suction pump 26. The cover body 2 and the housing 1 are hinged at one end, and the pipeline passes through the hinged end, but it does not affect the flow performance.

[0045] Preferably, the A liquid storage box 501, the B liquid storage box 502, and the distilled water storage box 503 are all transparent boxes, which are convenient for observing the liquid level, and can be pulled open or closed relative to the side of the housing 1 in a drawer-type structure; a circle of sealing gaskets 505 is provided on the cover 2 corresponding to each group of dyeing liquid tanks. The design of the sealing gasket 505 forms a relatively sealed cavity structure, which can obtain a better drainage effect when the drainage pump 59 starts suction, and can also prevent water from splashing to the adjacent dyeing tanks 5 groups during the flushing process.

[0046] Preferably, each dyeing tank 5 is provided with an A dye addition hole 506 and a B dye addition hole 507 on the upper edge, and the A dye addition hole 506 and the B dye addition hole 507 are arranged opposite to each other; each dyeing tank 5 is provided with two water vapor nozzles 21, and the two water vapor nozzles 21 are arranged along the length direction of the smear 6, and have an inclination design, and are arranged in an obliquely opposite manner. Such an inclination design makes the fluid entering through the water vapor nozzle 21 have a relative design structure. In the dye mixing working mode, the intermittent continuous airflow contacts the dye liquid surface in the inclined direction, which can push the dye to move in the opposite direction and promote the mixing of the dye.

[0047] Preferably, the control panel 3 can independently control the independent start and stop of each set of water-gas co-pipeline, dye liquid supply pipeline, and drainage pipeline; or the synchronous start and stop of three sets of water-gas co-pipeline, dye liquid supply pipeline, and drainage pipeline. The control panel 3 also includes a timer, which is connected to the control panel 3 and the display control panel 4. The display control panel 4 is used to display the current operation mode and operation time, and can input control instructions. The control panel 3 can be used to control the operation or stop of the corresponding pipeline according to the time axis to complete the process of automatic dye addition, mixing, mixing, dilution, drainage, cleaning, and drying. The external power cord or built-in battery of the power module, the built-in battery is arranged in the housing 1 below the display control panel 4.

[0048] In actual use: prepare the smear 6 to be stained, and select the device preset program in the preset program before use. At this time, the dye is pumped in advance to expel the air in the liquid supply pipe, so that the A dye and the B dye fill the liquid supply pipe; the excess A dye and the B dye are sucked away from the drainage hole 51, and the drainage pump 59 or the drainage solenoid valve at the end of the drainage pipe remains locked, and a sealed space is formed in the drainage pipe. The aperture of the drainage hole 51 is relatively small, and the dye does not automatically flow into the drainage hole 51 when it enters; after the preparation program is completed, the operator manually puts in the smear 6 to be stained, closes the cover 2, ensures that there is enough dye and clean water in the dye box, and inputs the start designation through the display control panel 4, and the micro-supply pump 53 of the A dye is started in advance. , add liquid A. After liquid A is added, the timer counts at the same time and continues for a fixed time of 30 seconds to 60 seconds to soak the smear 6. Subsequently, when liquid B is added, the micro liquid supply pump 53 in the corresponding liquid B box is started to add a specific amount of liquid B relative to liquid A at a ratio of 1:2-1:1 to each group of liquid B tanks to achieve mixing of liquids A and B. After liquid B is added, the control board 3 can control the suction pump 26 to start intermittently, and the three-way switching valve 24 pumps the gas sucked in by the air inlet 25 into each water gas nozzle 21 of the first-level water-gas common pipe 27 and the second-level water-gas common pipe 28, forming a number of intermittent airflows, and blowing air intermittently from both ends of the liquid dye tank to generate air push for the liquid dye, so as to achieve the effect of mixing the liquid A and liquid B. After the mixing and blowing process is completed, after 10-20 minutes, the three-way switching valve 24 is switched, the air inlet 25 is closed, the water pipe 22 is opened, and the suction pump 26 is started again to pump pure water into the water-gas common pipe to form a flushing process. At the same time, the drainage pump 59 on the drainage pipe starts suction, and the drainage pipe forms a negative pressure and starts suction, so that the dye in the dye tank is sucked into the drainage pipe through the drainage hole 51 to realize waste liquid discharge, and the dye tank is flushed with clean water until it is clean and then stopped. After the flushing waste water is discharged, the three-way switching valve 24 is switched again, the water pipe 22 is closed, the air inlet 25 is opened, and air is injected into the water-gas common pipe to realize continuous airflow blowing inside the dye tank for drying or drying. After completion, the cover body 2 can be manually opened to take out the dyed smear 6.

[0049] It should be understood that the above specific embodiments of the present invention are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation on the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. In addition, the claims attached to the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the attached claims, or the equivalent forms of such scope and boundaries.

Claims

1. An automatic staining device for peripheral blood smear and bone marrow smear, comprising a housing (1), a cover (2), a liquid storage box installed inside the housing (1), a control panel (3), a display control panel (4), and a staining chamber for placing a smear (6), characterized in that Also includes: There are a plurality of dyeing troughs (5), each row or column being divided into a group, and the groups are placed side by side in the dyeing chamber, each smear (6) is placed in a dyeing trough (5), at least one liquid material adding hole is opened on the inner wall of the upper edge of each dyeing trough (5), and a drainage hole (51) is arranged on the bottom of the dyeing trough (5); There are a plurality of water vapor nozzles (21) arranged on the inner top wall of the cover body (2), and the number and position of the distribution are adapted to the number and position of the dyeing slots (5), so that when the cover body (2) covers the shell (1), the dyeing chamber can be covered from above, and each water vapor nozzle (21) corresponds to a dyeing slot (5), and is used to spray clean water to clean the dyeing slot (5) and spray air to dry the dyeing slot (5); A pipeline system is used for supplying and draining liquid to each set of dyeing tanks (5), each set of the pipeline system comprising a set of dyeing liquid supply pipelines, a set of water and gas co-pipe pipelines, and a set of drainage pipelines; The dye liquid supply pipeline provides the dyeing tank (5) of each group with liquid A and liquid B; The liquid discharge pipeline provides a channel for discharging the dye liquid and a suction power for the dyeing tank (5) of each group; The water-air co-pipeline provides clean water to each group of dyeing tanks (5), as well as spraying and flushing and airflow drying; The control panel (3) is connected to and controls the pipeline system and the display and control panel (4); the power supply module is connected to the control panel (3), the pipeline system and the display and control panel (4) and is powered by wires.

2. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 1, characterized in that: The dye liquid supply pipeline comprises: one end of a primary liquid supply pipe (52) is connected to a micro liquid supply pump (53) in a liquid storage box, the other end of the primary liquid supply pipe (52) is connected to an N-type through-piece (54), and one end of a plurality of secondary liquid supply pipes (55) of equal length are respectively connected through the N-type through-piece (54), and the other end of the secondary liquid supply pipe (55) is connected to the liquid material adding hole of each dyeing tank (5); the liquid storage box comprises two, which are used for dispensing dye liquid A and dye liquid B, each liquid storage box is equipped with a plurality of micro liquid supply pumps (53), and each micro liquid supply pump (53) supplies liquid to a corresponding set of dye liquid supply pipelines; The water-gas co-pipeline comprises: one end of a water body pipeline (22) is connected to the bottom of the water box, and the other end is connected to a first interface of a three-way switching valve (24); the second interface of the three-way switching valve (24) is an air inlet (25); the second interface of the three-way switching valve (24) is connected to a suction pump (26); the outlet end of the suction pump (26) is connected to a primary water-gas co-pipeline (27); the upper branch of the primary water-gas co-pipeline (27) is connected to one end of a plurality of secondary water-gas co-pipes (28); the other end of the secondary water-gas co-pipes (28) is connected to each of the water-gas nozzles (21); each set of water-gas co-pipelines corresponds to a group of dyeing tanks (5); The drainage pipeline comprises: one end of a plurality of third-level drainage pipes (56) is connected to the drainage hole (51) at the bottom of each dyeing tank (5), and the other end is connected to the second-level drainage pipe (57); the end of the second-level drainage pipe (57) is connected to the first-level drainage pipe (58), and the end of the first-level drainage pipe (58) is connected to a drainage pump (59), and the drainage pump (59) provides negative pressure in the pipeline through suction to discharge waste liquid and waste water.

3. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 2, characterized in that: The dyeing tanks (5) are arranged in three groups side by side, and each group is composed of six dyeing tanks (5) arranged vertically in parallel; the liquid storage boxes include an A liquid storage box (501), a B liquid storage box (502), and a distilled water storage box (503), and are installed in the lower part of the shell (1) in a transverse insertion installation manner, wherein the A liquid storage box (501) and the B liquid storage box (502) are inserted from one side of the shell (1), and the distilled water storage box (503) is inserted from the other side; three micro liquid supply pumps (53) are respectively arranged in the A liquid storage box (501) and the B liquid storage box (502), and the micro liquid supply pumps (53) are installed on the end of the primary liquid supply pipe (52); the three groups of dyeing tanks (5) respectively correspond to a set of water and gas common pipes, a set of dye liquid supply pipes, and a set of drainage pipes.

4. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 2, characterized in that: The bottom of the dyeing tank (5) is an inclined tank which is gradually inclined toward the center. A slot is arranged at the top of one side of the dyeing tank (5), and the slot is convenient for taking or placing the smear (6) from one end. Two supporting strips (504) are arranged horizontally in the middle of the dyeing tank (5) for horizontally supporting the smear (6). A liquid discharge solenoid valve is installed on the first-level liquid discharge pipe (58), and the liquid discharge solenoid valve is connected to the control board (3).

5. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 2, characterized in that: The three-way switching valve (24), the suction pump (26), the primary water-gas common pipe (27) and the secondary water-gas common pipe (28) are all installed in the cover body (2), and a breathable mesh is provided on the top of the cover body (2) above the air inlet (25) of the suction pump (26).

6. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 3, characterized in that: The A liquid storage box (501), the B liquid storage box (502), and the distilled water storage box (503) are all transparent boxes, which are convenient for observing the liquid level, and are all drawer-type structures that can be pulled open or closed relative to the side of the shell (1); a circle of sealing gaskets (505) is provided on the cover (2) corresponding to each group of dye liquid tanks.

7. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 3, characterized in that: Each dyeing tank (5) is provided with an A dye liquid adding hole (506) and a B dye liquid adding hole (507) on the upper edge, and the A dye liquid adding hole (506) and the B dye liquid adding hole (507) are arranged opposite to each other; Each dyeing tank (5) is equipped with two water vapor nozzles (21), which are arranged along the length direction of the smear (6) and have an inclined design and are arranged in an obliquely opposite manner.

8. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 3, characterized in that: The control panel (3) can independently control the independent start and stop of each set of water-gas common pipes, dye liquid supply pipes, and drainage pipes; or the synchronous start and stop of three sets of water-gas common pipes, dye liquid supply pipes, and drainage pipes.

9. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 8, characterized in that: The device also includes a timer, which is connected to the control board (3) and the display control panel (4). The display control panel (4) is used to display the current operation mode and operation time and can input control instructions. The control board (3) can be used to control the operation or stop of the corresponding pipeline according to the time axis to complete the process of automatic dye liquid addition, mixing, mixing, dilution, drainage, cleaning and drying.

10. The automatic staining device for peripheral blood smear and bone marrow smear according to claim 1, characterized in that: The power module has an external power line or a built-in storage battery, and the built-in storage battery is arranged in the housing (1) below the display control panel (4).

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