Culture observation box for living cells

Abstract

The utility model discloses a culture observation box for living cells, which relates to the field of living cell detection devices and comprises a box body, a plurality of baffles, an upper cover plate, an air filter and a control main board, and the baffles arranged in an inner cavity of the box body divide the inner cavity of the box body into a plurality of independent chambers. The baffle between every two adjacent cavities is provided with a vent hole used for air circulation, the multiple air filters are arranged in the cavities of the box body at equal intervals, a taking and placing opening is formed in the position, located at the top of an inner cavity of the box body, of the upper portion of the cavity, and the upper cover plate is hinged to the top of the box body and used for being connected with the taking and placing opening in a sealed mode; the control main board is installed in any cavity, and the air filter is electrically connected with the control main board. According to the utility model, the inner cavity of the box body is divided into a plurality of independent chambers by adopting a plurality of baffles, so that various test instruments can be conveniently placed in a classified manner, the mutual influence among the instruments is reduced, meanwhile, the air circulation and filtration among the plurality of chambers are realized, and a stable and clean gas environment is provided for cell culture.

Description

Technical Field

[0001] The utility model relates to the field of living cell detection devices, in particular to a culture observation box for living cells. Background Art

[0002] Live cell imaging detection technology is a commonly used technology in the fields of biology and biomedicine, which is used to observe and record the structure, function, dynamic process and interaction of cells at the level of living cells. This technology can obtain images and data in real time in the state of living cells, providing a powerful tool for studying cell biology, cell signal transduction, drug development and other fields.

[0003] In order to enable cells to grow for a long time in vitro, the cell culture environment must be strictly controlled to reduce the impact of harmful substances on living cells. When observing and detecting living cells, the existing technology mostly adopts the operation method of placing the instrument in a cell culture box or adding a small culture dish or perfusion system for maintaining living cells to an instrument such as a microscope. The detection instrument is placed in a cell culture box. The instrument itself is a source of pollution and heat, which will cause great interference to the environment in the incubator and increase the probability of contamination. The solution of adding a small culture dish or perfusion system for maintaining living cells to an instrument such as a microscope will also cause problems such as incompatibility with the original instrument system and the need for another control system. No matter which of the above solutions is used, different instruments need to cooperate with each other, and cannot cooperate with each other under a set of control systems.

[0004] Therefore, how to develop a culture observation box for living cells, which uses a multi-baffle structure design to divide the inner cavity of the box into multiple independent chambers, so as to facilitate the classification and placement of various test instruments and reduce the mutual influence between instruments. At the same time, with the help of vents and air filters, the circulation and filtration of air between multiple chambers can be achieved to provide a stable and clean gas environment for cell culture. This has become a technical problem that needs to be urgently solved by people in this field. Utility Model Content

[0005] The purpose of the utility model is to provide a culture observation box for living cells, which adopts a multi-baffle structure design to divide the inner cavity of the box into multiple independent chambers, which is convenient for classifying and placing various test instruments and reducing the mutual influence between the instruments. At the same time, with the vents and air filters, the circulation and filtration of air between the multiple chambers are realized, providing a stable and clean gas environment for cell culture.

[0006] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0007] The utility model discloses a culture observation box for living cells, comprising a box body, a baffle, an upper cover, an air filter and a control main board, wherein a plurality of baffles arranged in the inner cavity of the box body divide the inner cavity of the box body into a plurality of independent chambers, and a vent hole for air circulation is provided on the baffle between each two adjacent chambers, a plurality of the air filters are arranged in the chamber of the box body at equal intervals, and a plurality of chambers for installing the air filters are located in the middle of the inner cavity of the box body, a take-in and put-out port is provided above the chamber located at the top of the inner cavity of the box body, the upper cover plate is hinged on the top of the box body and is used for sealing connection with the take-in and put-out port, the control main board is installed in any of the chambers, and the air filter is electrically connected to the control main board.

[0008] Preferably, the box body includes a box body and two box doors. A plurality of baffles arranged in the inner cavity of the box body divide the inner cavity of the box body into a multi-channel reagent pump installation cavity, a reagent storage cavity, a sample placement cavity, a controller installation cavity, a waste liquid pump installation cavity and a waste liquid storage cavity, a total of five chambers, which are respectively used to place corresponding medical devices. The front side of the box body is set to be an opening, and the two box doors are symmetrically hinged on the left and right sides of the box body. The access port is opened on the top of the box body, and the access port is communicated with the sample placement cavity.

[0009] Preferably, an LED transmission lamp is installed at the bottom of the upper cover plate, and the LED transmission lamp is electrically connected to the control main board.

[0010] Preferably, a placement groove for snap-fitting and placing a sample container box is provided at the bottom of the sample placement cavity, an observation through hole is provided at the center position of the bottom of the placement groove, a microscope imaging device for observing the sample container box is installed on the top of the controller installation cavity, and the objective lens end of the microscope imaging device shines toward the bottom of the sample container box through the observation through hole, and the microscope imaging device is electrically connected to the control main board.

[0011] Preferably, a plurality of the air filters are vertically spaced apart and arranged on the walls of the sample placement cavity and the controller installation cavity.

[0012] Compared with the prior art, the beneficial technical effects of the utility model are:

[0013] In general, the utility model adopts a multi-baffle structure design, which cleverly divides the inner cavity into multiple independent chambers, which is convenient for the classified placement of various test instruments. The chambers are connected by vents and are equipped with air filters to ensure that the air can circulate between the multiple chambers and be effectively filtered. This design creates a stable and clean gas environment for the sample container box. At the same time, the design of the access port greatly facilitates users to take samples. The upper cover can block dust, bacteria and other harmful substances, provide safe and stable storage conditions for the sample container box, and significantly reduce the impact of harmful substances on living cells in the sample container box. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The utility model will be further described below in conjunction with the accompanying drawings.

[0015] Figure 1 This is a schematic diagram of the overall structure of a culture observation box for living cells of the utility model;

[0016] Figure 2 It is a schematic diagram of the air flow direction in the inner cavity of the box of the utility model.

[0017] Explanation of the accompanying drawings: 1. Box body; 11. Box body; 12. Box door; 13. Multi-channel reagent pump installation cavity; 14. Reagent storage cavity; 15. Sample placement cavity; 16. Controller installation cavity; 17. Waste liquid pump installation cavity; 18. Waste liquid storage cavity; 2. Baffle; 3. Upper cover; 4. Air filter; 5. Access port; 6. LED transmission light; 7. Placement slot; 8. Observation through hole; 9. Vent hole. DETAILED DESCRIPTION

[0018] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0019] like Figure 1-2As shown, a culture observation box for living cells includes a box body 1, a baffle 2, an upper cover 3, an air filter 4 and a control main board. A plurality of baffles 2 arranged in the inner cavity of the box body 1 divide the inner cavity of the box body 1 into a plurality of independent chambers, and a vent 9 for air circulation is provided on the baffle 2 between each two adjacent chambers. A plurality of air filters 4 are arranged at equal intervals in the chamber of the box body 1, and a plurality of chambers for installing the air filters 4 are all located in the middle position of the inner cavity of the box body 1. A take-in and put-out port 5 is provided above the chamber located at the top of the inner cavity of the box body 1. The upper cover 3 is hinged at the top of the box body 1 and is used to be sealed and connected to the take-in and put-out port 5. The control main board is installed in any of the chambers, and the air filter 4 is electrically connected to the control main board.

[0020] Specifically, a plurality of the air filters 4 are vertically spaced apart and arranged on the walls of the sample placement cavity 15 and the controller installation cavity 16 .

[0021] Specifically, multiple independently arranged chambers achieve mutual air circulation through the air vents 9 opened on the baffle 2, and cooperate with the air filter 4 located at the center of the inner cavity of the box body 1, which not only effectively shortens the time required for air circulation and makes the air circulation more efficient, but also provides a stable and clean gas environment for the sample container box to ensure the accuracy of scientific research.

[0022] Specifically, the box body 1 includes a box body 11 and two box doors 12. A plurality of baffles 2 arranged in the inner cavity of the box body 11 divide the inner cavity of the box body 11 into a multi-channel reagent pump installation cavity 13, a reagent storage cavity 14, a sample placement cavity 15, a controller installation cavity 16, a waste liquid pump installation cavity 17 and a waste liquid storage cavity 18, a total of five chambers, which are respectively used to place corresponding test instruments. The front side of the box body 11 is set to be an opening, and the two box doors 12 are symmetrically hinged on the left and right sides of the box body 11. The access port 5 is opened at the top of the box body 11, and the access port 5 is communicated with the sample placement cavity 15.

[0023] Specifically, an LED transmission lamp 6 is installed at the bottom of the upper cover plate 3, and the LED transmission lamp 6 is electrically connected to the control main board.

[0024] Specifically, a placement groove 7 for clamping and placing a sample container box is provided at the bottom of the sample placement cavity 15, and an observation hole 8 is provided at the center position of the bottom of the placement groove 7. A microscope imaging device for observing the sample container box is installed on the top of the controller installation cavity 16, and the objective lens end of the microscope imaging device shines toward the bottom of the sample container box through the observation hole 8, and the microscope imaging device is electrically connected to the control main board.

[0025] Specifically, the multi-channel reagent pump installation cavity 13 is used to install a multi-channel reagent pump, and the reagent storage cavity 14 is used to place reagent bottles containing different reagents, and the sample container box, the multi-channel reagent pump and the reagent bottles are connected in sequence through multiple independent pipes; the waste liquid pump installation cavity 17 is used to install a waste liquid pump, and the waste liquid storage cavity 18 is used to place a waste liquid bottle, and the sample container box, the waste liquid pump and the waste liquid bottle are connected in sequence through pipes, and the multi-channel reagent pump and the waste liquid pump are electrically connected to the control main board.

[0026] The use process of the utility model is as follows:

[0027] First, open the box door 12, place or install the sample container box, multi-channel reagent pump, reagent bottle, waste liquid pump, waste liquid bottle, air filter 4 and microscope imaging device in the corresponding independent chamber, and close the box door 12 after all devices are set up, so that the inner cavity of the box body 11 forms a closed environment;

[0028] Then, open the air filter 4 to start filtering the air in the inner cavity of the box body 11. The air filter 4 is set at the center of the inner cavity of the box body 11, and cooperates with the vent holes 9 opened on the baffle 2 to realize the circulation and filtration of the air in multiple independent chambers, providing a stable and clean gas environment for the sample container box to ensure the accuracy of scientific research.

[0029] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0030] The embodiments described above are only descriptions of the preferred methods of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the claims of the present invention.

Claims

1. A culture observation box for living cells, characterized in that: The invention comprises a housing (1), a baffle (2), an upper cover (3), an air filter (4) and a control main board. A plurality of baffles (2) arranged in the inner cavity of the housing (1) divide the inner cavity of the housing (1) into a plurality of independent chambers, and a vent (9) for air circulation is provided on the baffle (2) between each two adjacent chambers. A plurality of air filters (4) are arranged at equal intervals in the chamber of the housing (1), and a plurality of chambers for installing the air filters (4) are located in the middle of the inner cavity of the housing (1). A take-in and take-out opening (5) is provided above the chamber located at the top of the inner cavity of the housing (1). The upper cover (3) is hinged to the top of the housing (1) and is used to be sealed and connected to the take-in and take-out opening (5). The control main board is installed in any of the chambers, and the air filter (4) is electrically connected to the control main board.

2. A culture observation box for living cells according to claim 1, characterized in that: The box body (1) comprises a box body (11) and two box doors (12). A plurality of baffles (2) arranged in the inner cavity of the box body (11) divide the inner cavity of the box body (11) into a multi-channel reagent pump installation cavity (13), a reagent storage cavity (14), a sample placement cavity (15), a controller installation cavity (16), a waste liquid pump installation cavity (17) and a waste liquid storage cavity (18), a total of five chambers, which are respectively used to place corresponding test instruments. The front side of the box body (11) is set as an opening, and the two box doors (12) are symmetrically hinged on the left and right sides of the box body (11). The access opening (5) is opened at the top of the box body (11), and the access opening (5) is connected to the sample placement cavity (15).

3. A culture observation box for living cells according to claim 2, characterized in that: An LED transmission lamp (6) is installed at the bottom of the upper cover plate (3), and the LED transmission lamp (6) is electrically connected to the control main board.

4. A culture observation box for living cells according to claim 2, characterized in that: The bottom of the sample placement cavity (15) is provided with a placement groove (7) for clamping and placing the sample container box, and the center position of the bottom of the placement groove (7) is provided with an observation through hole (8). A microscope imaging device for observing the sample container box is installed on the top of the controller installation cavity (16), and the objective lens end of the microscope imaging device shines toward the bottom of the sample container box through the observation through hole (8), and the microscope imaging device is electrically connected to the control main board.

5. The culture observation box for living cells according to claim 2, characterized in that: A plurality of the air filters (4) are vertically spaced apart and arranged on the walls of the sample placement chamber (15) and the controller installation chamber (16).

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