Immunofluorescence cassette

Abstract

The utility model discloses an immunofluorescence cassette which comprises a bottom plate, two connecting columns are fixedly connected to the position, close to the front side, of the top of the bottom plate, two connecting plates are fixedly connected to the position, close to the rear side, of the top of the bottom plate, connecting holes are formed in the connecting plates, a placing seat is fixedly connected to the position, close to the center position, of the top of the bottom plate, and the placing seat is fixedly connected with the bottom plate. According to the immunofluorescence cassette disclosed by the utility model, when a plurality of immunofluorescence cassettes need to form an experimental control group, after the first immunofluorescence cassette is placed well, the second immunofluorescence cassette is moved, so that the connecting columns penetrate through the connecting holes, and then the experimental control group is formed. Compared with the prior art, when the box cover of the device is opened, the connection between the two immunofluorescence cassettes does not need to be unfastened, and the device is simple to operate and suitable for popularization and application, and the box cover of one of the immunofluorescence cassettes can be independently opened by only moving the box cover upwards. And compared with the prior art, when the box cover of the device is opened, the connection between the two immunofluorescence cassettes does not need to be unfastened.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture vessels, in particular to an immunofluorescence dark box. Background Art

[0002] Cellular immunofluorescence technology is a technology that uses the principle of specific binding between intracellular antigens and antibodies to label antibodies with fluorescent pigments to locate, qualitatively and quantitatively study intracellular antigens under a microscope.

[0003] Cell immunofluorescence technology usually uses the method of cell climbing to observe under an upright microscope. Since the immunofluorescence experiment needs to be strictly protected from light during operation, there are immunofluorescence dark boxes on the market specifically for placing slides. However, in view of the complex steps of cell climbing and the difficulty of removing the cover glass from the culture vessel, in actual operation, the cells in the culture vessel (culture dish, culture plate) can also be directly placed under an inverted microscope for observation after staining. In particular, when performing immunofluorescence staining on living cells, culture vessels are more suitable than slides.

[0004] The existing Chinese patent CN215180242U discloses an immunofluorescence cassette. In order to form an experimental control group, a splicing buckle and a slot are provided on the cassette cover to solve the above problem. Two adjacent immunofluorescence cassettes can be connected by the splicing buckle and the slot. However, according to the above connection method, when it is necessary to open the cover of one of the immunofluorescence cassettes, it is necessary to first unfasten the fixation of the buckle and the slot before opening the cover of the immunofluorescence cassette. This operation method is extremely inconvenient and is not suitable for popularization and use. Utility Model Content

[0005] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide an immunofluorescence dark box.

[0006] One of the purposes of the utility model is achieved by the following technical solution:

[0007] An immunofluorescence dark box comprises a bottom plate, wherein the top of the bottom plate is fixedly connected to two connecting columns near the front side, the top of the bottom plate is fixedly connected to two connecting plates near the rear side, connecting holes are provided on the connecting plates, the top of the bottom plate is fixedly connected to a placement seat near the center position, the top of the placement seat is movably sleeved with a box cover, the top of the placement seat is provided with a first placement groove and a second placement groove, a first slot is provided between the first placement groove and the second placement groove, the left and right sides of the placement seat are provided with second slots, the inner cavity of the second slot is provided with a movable rod, a rotating shaft is movably penetrated through the movable rod, two clamping blocks are fixedly connected to the side of the movable rod close to the first slot, two springs are fixedly connected to the side of the movable rod away from the clamping blocks, and the springs are fixedly connected to the bottom of the inner cavity of the second slot.

[0008] Furthermore, the two connecting columns and the connecting plate are bilaterally symmetrically arranged with the central axis of the bottom plate as the symmetry axis, the cross section of the connecting plate is L-shaped, and the connecting holes match the connecting columns.

[0009] Furthermore, the first placement groove and the second placement groove have inner cavities movably connected thereto with a first culture dish and a second culture dish, respectively.

[0010] Furthermore, the rotating shaft is fixedly connected to the inner cavity of the second slot, and the cross section of the movable rod is U-shaped, with the U-shaped opening facing upward.

[0011] Furthermore, the bottoms of the four card blocks are respectively attached to the tops of the first culture dish and the second culture dish.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. The utility model discloses an immunofluorescence cassette. When a plurality of immunofluorescence cassettes need to form an experimental control group, the first immunofluorescence cassette is placed first, and then the second immunofluorescence cassette is moved so that the connecting column passes through the connecting hole, thereby completing the connection between the two immunofluorescence cassettes. At this time, the cover of one of the immunofluorescence cassettes can be opened separately by simply moving the cover upward. Compared with the prior art, when the device opens the cover, there is no need to untie the connection between the two immunofluorescence cassettes, so the operation is simple and suitable for popularization and use.

[0014] 2. The utility model provides an immunofluorescence dark box. When the first culture dish and the second culture dish need to be taken out, two hands are placed on the first slot and the second slot inner cavity respectively, and then the movable rod is pushed. The movable rod rotates around the rotating shaft, and the movable rod drives the card block to flip away from the first culture dish or the second culture dish. At the same time, the reed is deformed to release the fixation of the first culture dish or the second culture dish, and then the first culture dish or the second culture dish is moved upward. The operation is convenient.

[0015] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the utility model more obvious and easy to understand, the following preferred embodiments are specifically cited and described in detail with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a three-dimensional diagram of this embodiment;

[0017] Figure 2 It is a sectional perspective view of the component box cover of this embodiment;

[0018] Figure 3 A three-dimensional diagram of the component placement seat of this embodiment;

[0019] Figure 4 An exploded view of the component placement seat, the first culture dish and the second culture dish of this embodiment;

[0020] Figure 5 For this embodiment Figure 3 The enlarged view of point A in the middle;

[0021] Figure 6 It is a three-dimensional diagram of the movable rod of the present embodiment.

[0022] In the figure: 1, bottom plate; 2, connecting column; 3, connecting plate; 4, connecting hole; 5, placement seat; 6, box cover; 7, first placement slot; 8, second placement slot; 9, first slot; 10, first culture dish; 11, second culture dish; 12, second slot; 13, movable rod; 14, rotating shaft; 15, spring; 16, block. DETAILED DESCRIPTION

[0023] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0024] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may also be a component centered. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may also be a component centered. When a component is considered to be "set on" another component, it may be directly set on the other component or there may also be a component centered. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0026] Please refer to Figures 1 to 6, an immunofluorescence dark box, comprising a bottom plate 1, two connecting columns 2 are fixedly connected to the top of the bottom plate 1 near the front side, two connecting plates 3 are fixedly connected to the top of the bottom plate 1 near the rear side, a connecting hole 4 is opened on the connecting plate 3, the two connecting columns 2 and the connecting plate 3 are symmetrically arranged with the central axis of the bottom plate 1 as the symmetry axis, the cross section of the connecting plate 3 is L-shaped, the connecting hole 4 and the connecting column 2 match each other, a placement seat 5 is fixedly connected to the top of the bottom plate 1 near the center position, a box cover 6 is movably sleeved on the top of the placement seat 5, a first placement groove 7 and a second placement groove 8 are opened on the top of the placement seat 5, and the inner cavities of the first placement groove 7 and the second placement groove 8 are respectively movably plugged with a first culture dish 10 and a second culture dish 11;

[0027] A first slot 9 is provided between the first placement slot 7 and the second placement slot 8, and second slots 12 are provided on both the left and right sides of the placement seat 5. A movable rod 13 is provided in the inner cavity of the second slot 12, and a rotating shaft 14 is movably provided on the movable rod 13 and penetrates therethrough. The rotating shaft 14 is fixedly connected to the inner cavity of the second slot 12. The cross-section of the movable rod 13 is U-shaped, and the U-shaped opening faces upward. Two blocks 16 are fixedly connected to the side of the movable rod 13 close to the first slot 9, and two springs 15 are fixedly connected to the side of the movable rod 13 away from the block 16. The springs 15 are fixedly connected to the bottom of the inner cavity of the second slot 12, and the bottoms of the four blocks 16 are respectively fitted with the tops of the first culture dish 10 and the second culture dish 11.

[0028] Working principle: The utility model is an immunofluorescence cassette. During use, when multiple immunofluorescence cassettes need to form an experimental control group, the first immunofluorescence cassette is placed first, and then the second immunofluorescence cassette is moved, and the connecting hole 4 on the second immunofluorescence cassette is aligned with the connecting column 2 on the first immunofluorescence cassette, and then the second immunofluorescence cassette is moved downward so that the connecting column 2 passes through the connecting hole 4, thereby completing the connection between the two immunofluorescence cassettes. At this time, the box cover 6 of one of the immunofluorescence cassettes can be opened separately, and it only needs to move the box cover 6 upward. Compared with the prior art, when the device opens the box cover 6, there is no need to untie the connection between the two immunofluorescence cassettes, the operation is simple, and it is suitable for popularization and use;

[0029] When it is necessary to place the first culture dish 10 and the second culture dish 11, the box cover 6 is first moved upward so that the box cover 6 moves to the placement seat 5, and then the first culture dish 10 is moved, and the first culture dish 10 and the second culture dish 11 are moved to the first placement groove 7 and the second placement groove 8 respectively, and then the first culture dish 10 and the second culture dish 11 are moved downward until they touch the adjacent blocks 16 respectively, and then as the first culture dish 10 and the second culture dish 11 continue to move downward, the first culture dish 10 and the second culture dish 11 push the blocks 16, so that the movable rod 13 turns around the rotating shaft 14 as the center, and the spring plate 15 is deformed at the same time until the first culture dish 10 and the second culture dish 11 are respectively moved to the bottom of the inner cavity of the first placement groove 7 and the second placement groove 8 and fit each other, at this time, the movable rod 13 drives the blocks 16 to turn over under the elastic action of the spring plate 15, and the four blocks 16 are turned over to the first culture dish 10 and the second culture dish 11 respectively, and the blocks 16 fix the first culture dish 10 and the second culture dish 11;

[0030] When it is necessary to take out the first culture dish 10 and the second culture dish 11, two hands are placed in the first slot 9 and the second slot 12 respectively. The hand placed in the second slot 12 pinches the first culture dish 10 or the second culture dish 11 with the thumb and index finger, and then pushes the movable rod 13 with the middle finger. The movable rod 13 rotates around the rotating shaft 14, and the movable rod 13 drives the clamping block 16 to flip away from the first culture dish 10 or the second culture dish 11. At the same time, the reed 15 is deformed to release the fixation of the first culture dish 10 or the second culture dish 11, and then the first culture dish 10 or the second culture dish 11 is moved upward, which is convenient to operate.

[0031] The above-mentioned implementation modes are only preferred implementation modes of the present invention, and cannot be used to limit the protection scope of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. An immunofluorescence dark box, comprising a bottom plate (1), characterized in that: Two connecting columns (2) are fixedly connected to the top of the bottom plate (1) near the front side, two connecting plates (3) are fixedly connected to the top of the bottom plate (1) near the rear side, and connecting holes (4) are provided on the connecting plates (3). A placement seat (5) is fixedly connected to the top of the bottom plate (1) near the center, and a box cover (6) is movably sleeved on the top of the placement seat (5). A first placement groove (7) and a second placement groove (8) are provided on the top of the placement seat (5), and the first placement groove (7) and the second placement groove (8) are connected to each other. A first slot (9) is provided between the two sides of the placement seat (5), and a second slot (12) is provided on both the left and right sides of the placement seat (5). A movable rod (13) is provided in the inner cavity of the second slot (12), and a rotating shaft (14) is movably penetrated on the movable rod (13). Two clamping blocks (16) are fixedly connected to the side of the movable rod (13) close to the first slot (9), and two spring leaves (15) are fixedly connected to the side of the movable rod (13) away from the clamping blocks (16), and the spring leaves (15) are fixedly connected to the bottom of the inner cavity of the second slot (12).

2. An immunofluorescence dark box as claimed in claim 1, characterized in that: The two connecting columns (2) and the connecting plate (3) are both arranged bilaterally symmetrically with the central axis of the bottom plate (1) as the symmetry axis; the cross section of the connecting plate (3) is L-shaped; and the connecting hole (4) matches the connecting column (2).

3. An immunofluorescence dark box as claimed in claim 1, characterized in that: The inner cavities of the first placement groove (7) and the second placement groove (8) are respectively movably connected with a first culture dish (10) and a second culture dish (11).

4. An immunofluorescence dark box as claimed in claim 1, characterized in that: The rotating shaft (14) is fixedly connected to the inner cavity of the second slot (12); the cross section of the movable rod (13) is U-shaped, and the U-shaped opening faces upward.

5. The immunofluorescence dark box according to claim 1, characterized in that: The bottoms of the four clamping blocks (16) are respectively fitted to the tops of the first culture dish (10) and the second culture dish (11).

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