Automatic microscope

By using a combination of phase plate and annular aperture in an automated microscope, the problem of low contrast and resolution when observing live cells, unstained samples or insignificant staining of samples is solved, and a clearer imaging effect is achieved.

CN222800937UActive Publication Date: 2025-04-25SHANDONG SHIDASI BIOLOGICAL IND CO LTD +1
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Patent Information

Application Number
CN202421768066.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-25
Publication Date
2025-04-25
Estimated Expiration
2034-07-25

AI Technical Summary

Technical Problem

When existing upright microscopes observe living cells, unstained samples or insignificant staining samples, the contrast between samples between the structures, low image resolution, and low imaging clarity, resulting in inaccurate recognition.

Method used

An automatic microscope is designed, including a camera, an objective lens assembly and a transmission assembly. The objective lens assembly is equipped with a number of objective lenses of different magnifications arranged in a linear manner. A phase plate is provided inside the 40X objective lens, and an annular aperture is provided above the light hole.

Benefits of technology

The contrast between sample structures is improved through the phase plate, and the scattered light is suppressed through the annular aperture, which improves the resolution and contrast of the sample image, making the imaging clearer, and being able to effectively observe live cells, unstained samples or non-stained samples.

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Abstract

The utility model relates to an automatic microscope, which comprises a camera, an objective lens assembly and a transmission assembly, the objective lens assembly comprises a plurality of objective lenses with different magnification times and a light through hole plate, the light through hole plate is provided with a plurality of light through holes corresponding to each objective lens, and the transmission assembly is used for driving and replacing the objective lenses with different magnification times. A phase plate is arranged in at least one of the objective lenses with different magnification times, and an annular diaphragm is arranged above the light through hole corresponding to the objective lens. According to the utility model, the contrast ratio between sample structures and the sample image resolution are effectively improved, so that the imaging is clearer, and living cells, undyed samples or unobviously dyed samples can be effectively observed.
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Description

Technical Field

[0001] The utility model relates to the technical field of microscopes, in particular to an automatic microscope. Background Art

[0002] The Chinese utility model patent with publication number CN215813539U discloses an upright microscope, including a C-shaped objective lens holder and a plurality of objective lenses of different magnifications arranged in a straight line, etc. The upright microscope is convenient for quickly and automatically switching to an objective lens of a specific magnification. However, there are the following defects: when using the existing upright microscope for sample observation, what can be seen are conventional plane images, the contrast between sample structures is low, the resolution of sample images is low, the imaging clarity is low, and there is a technical problem of inaccurate recognition of living cells, unstained samples or samples with unclear staining. Therefore, it is particularly important to develop an automatic microscope that can effectively observe living cells, unstained samples or samples with unclear staining. Summary of the invention

[0003] The technical problem to be solved by the utility model is to overcome the above-mentioned defects of the prior art and provide an automatic microscope. The automatic microscope effectively improves the contrast between sample structures and the resolution of sample images, makes the imaging clearer, and can effectively observe living cells, unstained samples or samples with unclear staining.

[0004] The utility model is realized by the following technical solutions:

[0005] An automatic microscope comprises a camera, an objective lens assembly and a transmission assembly. The objective lens assembly comprises a plurality of objective lenses with different magnifications arranged in a straight line and a light-through hole plate. The light-through hole plate has a plurality of light-through holes corresponding to each objective lens. The transmission assembly is used for driving the replacement of objective lenses with different magnifications. At least one of the plurality of objective lenses with different magnifications has a phase plate arranged inside, and an annular aperture is arranged above the light-through hole corresponding to the objective lens.

[0006] As an optimization, the annular aperture is opened on the annular aperture plate.

[0007] As an optimization, the annular aperture plate has an overall rectangular structure with a length of 40-70 mm, a width of 25-35 mm, and a height of 0.2 mm.

[0008] As an optimization, mounting holes are provided on the annular aperture plate.

[0009] As an optimization, three mounting holes are provided, two of which are respectively provided at two corners of the short side of the annular aperture plate, and the other one is provided at the middle of the other short side of the annular aperture plate.

[0010] As an optimization, a filter is fixedly installed above the annular aperture.

[0011] As an optimization, the filter is a green filter.

[0012] As an optimization, the filter is a square green light filter with a length and width of 20-30 mm.

[0013] As an optimization, three objective lenses of different magnifications are arranged in a straight line, from left to right, 100X objective lens, 10X objective lens and 40X objective lens, among which the 40X objective lens is equipped with a phase plate inside.

[0014] The beneficial effects of the utility model are:

[0015] The utility model provides an automatic microscope, including a camera, an objective lens assembly and a transmission assembly, wherein the objective lens assembly includes a plurality of objective lenses of different magnifications arranged in a straight line and a light-through hole plate, wherein the light-through hole plate has a plurality of light-through holes corresponding to each objective lens, and the transmission assembly is used to drive the replacement of objective lenses of different magnifications, wherein at least one objective lens among the plurality of objective lenses of different magnifications has a phase plate disposed therein, and an annular aperture is disposed above the light-through hole corresponding to the objective lens. The utility model effectively improves the contrast between sample structures and the sample image resolution, makes the imaging clearer, and can effectively observe living cells, unstained samples or samples with unclear staining. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] An automatic microscope is further described below in conjunction with the accompanying drawings:

[0017] Figure 1 It is a structural schematic diagram of some embodiments of an automatic microscope of the utility model;

[0018] Figure 2 It is a structural schematic diagram of other embodiments of an automatic microscope of the utility model;

[0019] Figure 3 yes Figure 2 Schematic diagram of the partial enlarged structure of part A in the middle.

[0020] In the figure: 1 is the objective lens, 2 is the phase plate, 3 is the light-clearing hole plate, 4 is the annular aperture plate, 5 is the annular aperture, 6 is the filter, and 7 is the mounting hole. DETAILED DESCRIPTION

[0021] The present application is further described in detail below in conjunction with the accompanying drawings and implementation methods. It is particularly noted that the following implementation methods are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following implementation methods are only some implementation methods of the present application rather than all implementation methods. All other implementation methods obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0022] The terms "first", "second" and "third" in this application are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined as "first", "second" and "third" can explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "multiple" is at least two, and the manner is such as two, three, etc., unless otherwise clearly and specifically defined. All directional indications in the implementation mode of this application (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication also changes accordingly. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions. The manner, such as a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally also includes steps or units that are not listed, or optionally also includes other steps or units inherent to these processes, methods, products or devices.

[0023] The terms "install", "connect", "connect", "fix" and the like in this application should be understood in a broad sense. For example, "connect" can be a fixed connection, a detachable connection, or an integral connection; "connect" can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0024] Reference to "embodiment" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiment may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0025] See also Figure 1 , Figure 1It is a structural schematic diagram of some embodiments of an automatic microscope of the utility model; an automatic microscope comprises a camera, an objective lens assembly and a transmission assembly, the objective lens assembly comprises three objective lenses 1 with different magnifications arranged in a straight line and a light-through hole plate 3, the light-through hole plate 3 has three light-through holes corresponding to each objective lens 1, the transmission assembly is used for driving the replacement of the objective lenses 1 with different magnifications, the three objective lenses 1 with different magnifications arranged in a straight line are, from left to right, a 100X objective lens, a 10X objective lens and a 40X objective lens, wherein a phase plate 2 is arranged inside the 40X objective lens, and an annular aperture 5 is arranged above the light-through hole corresponding to the 40X objective lens. In this design, by setting a phase plate inside the 40X objective lens, the optical path difference of the visible light passing through the specimen is converted into an amplitude difference, thereby improving the contrast between various structures of the specimen, making various structures clearly visible when not stained or not obviously stained; by setting an annular aperture, the scattered light directed to the specimen can be suppressed, thereby improving the resolution and contrast of the specimen image. At the same time, the light passing through the annular aperture will form a conical light cone, and the focus of the light cone will be concentrated on the specimen, so that only the central light from the specimen can pass through the lens, enhancing the depth of the focal plane of the lens and making the image clearer. It effectively improves the contrast between specimen structures and the resolution of specimen images, making the imaging clearer, and can effectively observe living cells, unstained specimens or specimens with unclear staining.

[0026] See also Figure 1 , Figure 1 It is a schematic diagram of the structure of some embodiments of an automatic microscope of the utility model; the annular diaphragm 5 is arranged on the annular diaphragm plate 4. Such a design facilitates the setting of the annular diaphragm.

[0027] See also Figure 1 , Figure 1 The structure diagram of some embodiments of the automatic microscope of the utility model is shown in FIG. 4 ; the annular aperture plate 4 is a rectangular parallelepiped structure as a whole, with a length of 40-70 mm, a width of 25-35 mm, and a height of 0.2 mm. Such a design is easy to process and has a good effect.

[0028] See also Figure 1 , Figure 1 The utility model is a schematic diagram of the structure of some embodiments of an automatic microscope; the annular aperture plate 4 is provided with a mounting hole 7. Such a design facilitates the annular aperture plate to be mounted on the light-transmitting hole plate.

[0029] See also Figure 1 , Figure 1 It is a structural schematic diagram of some embodiments of an automatic microscope of the utility model; three mounting holes 7 are provided, two of which are respectively provided at two corners of the short side of the annular aperture plate 4, and the other one is provided at the middle of the other short side of the annular aperture plate 4. With such a design, a triangular positioning structure is formed, and the annular aperture plate is more firmly installed.

[0030] See also Figure 2 , Figure 3 , Figure 2 It is a structural schematic diagram of other embodiments of an automatic microscope of the utility model; Figure 3 yes Figure 2 A partial enlarged structural diagram of the middle A part; a filter 6 is fixedly installed above the annular aperture 5. In this design, by setting the filter, the wavelength range of the illumination light passing through the sample can be narrowed, the phase change caused by the different wavelengths of the illumination light can be reduced, and the contrast between the sample structures can be improved.

[0031] See also Figure 2 , Figure 3 , Figure 2 It is a structural schematic diagram of other embodiments of an automatic microscope of the utility model; Figure 3 yes Figure 2 The partial enlarged structural diagram of the middle A part; the filter 6 is a green filter. Such a design has a better effect.

[0032] See also Figure 2 , Figure 3 , Figure 2 It is a structural schematic diagram of other embodiments of an automatic microscope of the utility model; Figure 3 yes Figure 2 The partial enlarged structural diagram of the middle A part; the filter 6 is a square green light sheet, the length and width are both 20-30mm. Such a design is easy to process and install, and the effect is better.

[0033] Different from the prior art, the present application provides an automatic microscope, including a camera, an objective lens assembly and a transmission assembly, wherein the objective lens assembly includes a plurality of objective lenses of different magnifications arranged in a straight line and a light-through hole plate, wherein the light-through hole plate has a plurality of light-through holes corresponding to each objective lens, and the transmission assembly is used to drive the replacement of objective lenses of different magnifications, wherein at least one objective lens of the plurality of objective lenses of different magnifications has a phase plate inside, and an annular aperture is provided above the light-through hole corresponding to the objective lens. The utility model effectively improves the contrast between sample structures and the resolution of sample images, makes imaging clearer, and can effectively observe living cells, unstained samples or samples with unclear staining.

[0034] The above description shows the main features, basic principles, and advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments or examples, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, the above embodiments or examples should be regarded as exemplary and non-restrictive. The scope of the utility model is defined by the attached claims rather than the above description, so it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the utility model. Any figure mark in the claims should not be regarded as limiting the claims involved. Any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the utility model without departing from the technical principle of the utility model shall fall within the scope of patent protection of the utility model.

Claims

1. An automatic microscope, comprising a camera, an objective lens assembly and a transmission assembly, wherein the objective lens assembly comprises a plurality of objective lenses of different magnifications arranged in a straight line and a light-through hole plate, wherein the light-through hole plate has a plurality of light-through holes corresponding to each objective lens, and the transmission assembly is used to drive the replacement of objective lenses of different magnifications, characterized in that: At least one of the multiple objective lenses with different magnifications is provided with a phase plate inside, and an annular aperture is provided above the light aperture corresponding to the objective lens.

2. An automatic microscope as claimed in claim 1, characterized in that: The annular aperture is arranged on the annular aperture plate.

3. An automatic microscope as claimed in claim 2, characterized in that: The annular aperture plate is in a rectangular parallelepiped structure as a whole, with a length of 40-70 mm, a width of 25-35 mm, and a height of 0.2 mm.

4. An automatic microscope as claimed in claim 3, characterized in that: The annular aperture plate is provided with a mounting hole.

5. An automatic microscope as claimed in claim 4, characterized in that: There are three mounting holes, two of which are respectively arranged at two corners of the short side of the annular aperture plate, and the other one is arranged in the middle of the other short side of the annular aperture plate.

6. An automatic microscope according to any one of claims 1 to 5, characterized in that: A filter is fixedly installed above the annular aperture.

7. An automatic microscope as claimed in claim 6, characterized in that: The filter is a green filter.

8. An automatic microscope as claimed in claim 6, characterized in that: The filter is a square green light filter with a length and a width of 20-30 mm.

9. An automatic microscope as claimed in claim 1, characterized in that: Three objective lenses of different magnifications are arranged in a straight line, which are a 100X objective lens, a 10X objective lens and a 40X objective lens from left to right, wherein the phase plate is arranged inside the 40X objective lens.

Citation Information

Patent Citations

  • Upright microscope

    CN215813539U