Fibroin gel shaping mold

Abstract

The utility model relates to a mould, in particular to a silk protein gel shaping mould, which is of a detachable double-layer structure and comprises an inner-layer mould, an outer-layer mould and a sealing end cover. The inner-layer mold is arranged in the outer-layer mold in a sleeving manner; holes are formed in the surface of the outer-layer mold; and the sealing end covers are assembled at the two ends of the inner-layer mold and the outer-layer mold, so that a closed cavity is formed in the inner-layer mold. Compared with the prior art, the silk protein gel forming mold has the advantages that the defect that the silk protein gel forming mold in the prior art is difficult to demold and cannot realize soaking treatment is overcome, and the silk protein gel forming and the soaking treatment of the formed gel can be realized in the same mold.

Description

Technical Field

[0001] The utility model relates to a mold, in particular to a silk protein gel shaping mold. Background Art

[0002] Silk protein (or fibroin) from natural silkworm cocoons is a natural biological protein material with the advantages of good biocompatibility, easy availability, good swelling and natural antibacterial properties. Foreign literature has reported the use of silk protein to make nail-plate bone fixation systems, and animal experiments have confirmed its good biocompatibility.

[0003] In the prior art, suitable composite materials and preparation methods have been proposed for the application of silk protein as bone nails and bone plates, such as CN111956871A discloses a silk protein / gelatin composite material and its use, which is prepared by a method comprising the following steps: 1) immersing silkworm cocoons in a soap salt solution to degumming and drying to obtain degummed silk, and then dissolving the degummed silk in a lithium bromide solution, dialyzing desalting, and concentrating to obtain a 10%wt regenerated silk protein solution, wherein the soap salt is sodium carbonate or sodium bicarbonate; 2) mixing the silk protein solution obtained in step 1) with a 30%wt gelatin solution in a volume ratio of 9:1, then adding horseradish peroxidase, hydrogen peroxide solution and glutamine transaminase, mixing and gelling to obtain a silk protein / gelatin gel. 3) Soaking the silk protein / gelatin gel obtained in step 2) in a 60-80% ethanol aqueous solution for 24-36 hours, and then soaking it in an ammonium sulfate solution for 8-20 hours to obtain a silk protein / gelatin cross-linked gel; 4) Drying the silk protein / gelatin cross-linked gel obtained in step 3) to obtain a silk protein / gelatin composite material.

[0004] Among them, for the above step 2), during the gelation process of silk protein / gelatin gel, the strength of the intermediate gel after chemical cross-linking with horseradish peroxidase and hydrogen peroxide solution is relatively weak, and it is difficult to demold and transfer it as a whole in existing test tubes, centrifuge tubes and other containers; secondly, in step 3), the gel is soaked in ethanol aqueous solution and ammonium sulfate solution to induce the formation of β-folding inside the silk protein and strengthen the gelatin branch chain modification. During the soaking process, there is a lack of a device to maintain the stability of the gel shape; therefore, it is necessary to design a silk protein mold that integrates gel shaping, demolding, and soaking treatment to avoid structural damage caused by the transfer of the intermediate gel, and to ensure the stability of the shape of the gel during the soaking process, which is convenient for subsequent stabilization production. Utility Model Content

[0005] The purpose of the present utility model is to provide a silk protein gel shaping mold in order to solve at least one of the above problems, so as to overcome the defects in the prior art that the silk protein gel shaping mold is difficult to demould and cannot be immersed in the mold. The present invention can achieve the shaping of the silk protein gel and the immersion treatment of the gel after shaping in the same mold.

[0006] The purpose of the utility model is achieved through the following technical solutions:

[0007] A silk protein gel shaping mold, the shaping mold is a detachable double-layer structure, comprising an inner layer mold, an outer layer mold and a sealing end cover;

[0008] The inner layer mold is sleeved inside the outer layer mold;

[0009] The surface of the outer layer mold has holes;

[0010] The sealing end caps are assembled at both ends of the inner layer mold and the outer layer mold, so that a closed cavity is formed inside the inner layer mold.

[0011] Preferably, the surface of the outer layer mold is provided with openings spaced apart along the circumferential direction.

[0012] Preferably, the surface of the outer mold is provided with openings spaced apart along the axial direction.

[0013] Preferably, the surface openings of the outer layer mold are array holes.

[0014] The opening can be a round hole or a slotted hole.

[0015] Preferably, the outer layer mold and the inner layer mold are arranged concentrically.

[0016] Preferably, the sealing end cover is threadedly connected to the outer mold.

[0017] Preferably, a positioning slot is provided on the sealing end cover, and the end of the inner layer mold is inserted into the positioning slot to form a plug-in connection with the sealing end cover.

[0018] Preferably, a sealing member is provided in the positioning slot.

[0019] Preferably, a positioning slot is provided on the sealing end cover, and the end of the inner layer mold is inserted into the positioning slot to form a snap connection with the sealing end cover.

[0020] Preferably, a sealing member is provided in the positioning slot.

[0021] The working principle of the utility model is:

[0022] When in use, the inner layer mold is first inserted into the inner layer mold, and the sealing end cap at one end is covered to seal the inner layer mold and the outer layer mold, and the sealing end cap at the other end is covered and sealed after adding the silk protein gel raw material into the inner layer mold, so that the silk protein gel is shaped inside the inner layer mold. After shaping, the inner layer mold is taken out from any end and re-covered and sealed, and the shaping mold that takes out the inner layer mold is immersed in a soaking liquid (such as ethanol, ammonium sulfate, etc.) as a whole to implement soaking treatment, and the soaking liquid enters the interior of the shaping mold from the opening of the outer layer mold and contacts with the silk protein gel. After completing the soaking treatment, the silk protein gel blank is taken out from the shaping mold and dried.

[0023] Compared with the prior art, the utility model has the following beneficial effects:

[0024] Through the design of the double-layer structure, the silk protein can be shaped in the inner mold first, and after the shaping is completed, the inner mold is taken out and the shaping mold is immersed in the soaking liquid for treatment, and the soaking liquid is immersed in the shaping mold through the opening to contact the silk protein. Therefore, the shaping mold can complete shaping and soaking in the same mold. During the process, the silk protein is continuously in the shaping mold, and there is no need to move the silk protein body, so as to avoid damage to the structure and shape of the silk protein gel, and can also ensure the stability of the shape during the gel soaking process. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a structural schematic diagram of the shaping mold of Example 1;

[0026] Figure 2 It is a schematic cross-sectional view of the structure of the shaping mold of Example 1;

[0027] Figure 3 It is a structural schematic diagram of the shaping mold of Example 2;

[0028] Figure 4 It is a schematic cross-sectional view of the structure of the shaping mold of Example 2;

[0029] In the figure: 1-inner mold; 2-outer mold; 3-sealing end cover. DETAILED DESCRIPTION

[0030] The utility model is described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Example 1

[0032] A silk protein gel shaping mold, such as Figure 1-2 As shown, the shaping mold is a detachable double-layer structure, including an inner mold 1, an outer mold 2 and a sealing end cover 3;

[0033] The inner layer mold 1 is sleeved inside the outer layer mold 2;

[0034] The surface of the outer layer mold 2 is opened;

[0035] The sealing end caps 3 are assembled at both ends of the inner mold 1 and the outer mold 2 , so that a closed cavity is formed inside the inner mold 1 .

[0036] More specifically, in this embodiment:

[0037] The shaping mold consists of three parts: an outer mold 2, an inner mold 1 and a sealing end cover 3, wherein the inner mold 1 is sleeved inside the outer mold 2, the outer mold 2 has a hole on its surface, and the sealing end cover 3 covers both ends of the inner mold 1 and the outer mold 2.

[0038] The sealing end cap 3 and the outer layer mold 2 are detachably assembled by means of a threaded connection, while the sealing end cap 3 and the inner layer mold 1 are detachably assembled by means of a snap-on or plug-in connection. At the same time, after the sealing end cap 3 is assembled, a closed cavity can be formed inside the inner layer mold 1 for shaping the silk protein gel. Specifically, the inner wall surface of the sealing end cap 3 is provided with an internal thread, and the outer wall surface at the end position of the outer layer mold 2 is provided with an external thread, and the two threads cooperate with each other. A positioning slot or a positioning card slot is provided on the inner surface of the sealing end cap 3, and a sealing member (such as a sealing ring) is provided inside the positioning slot or the positioning card slot. By plugging or plugging the inner layer mold 1 into the positioning slot or the positioning card slot of the sealing end cap 3, the closed cavity inside the inner layer mold 1 is formed, such as Figure 2 .

[0039] The openings on the surface of the outer mold 2 are arranged in an array and spaced apart in the circumferential and axial directions, such as Figure 1 At the same time, the outer mold 2 and the inner mold 1 are arranged concentrically. This arrangement and the opening form can make the shaping mold more evenly contact when immersed in the soaking liquid, ensuring the soaking uniformity of the silk protein.

[0040] When in use, first, the inner mold 1 is inserted into the inner layer mold 2, and the sealing end cap 3 at one end is closed to seal the inner mold 1 and the outer mold 2, and the silk protein gel raw material is added to the inner mold 1, and the sealing end cap 3 at the other end is closed and sealed, so that the silk protein gel is shaped inside the inner mold 1. After shaping, the inner mold 1 is taken out from any end and re-sealed, and the shaping mold with the inner mold 1 taken out is immersed in a soaking liquid (such as ethanol, ammonium sulfate, etc.) as a whole to implement soaking treatment, and the soaking liquid enters the interior of the shaping mold from the opening of the outer mold 2 and contacts with the silk protein gel. After completing the soaking treatment, the silk protein is taken out from the shaping mold.

[0041] Example 2

[0042] The structure of the shaping mold in this embodiment is basically the same as that in the embodiment 1, the main difference is that the openings on the surface of the outer mold 2 are slot-shaped holes, such as Figure 3 , 4 shown.

[0043] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. A silk protein gel shaping mold, characterized in that: The shaping mold is a detachable double-layer structure, comprising an inner mold (1), an outer mold (2) and a sealing end cover (3); The inner layer mold (1) is sleeved inside the outer layer mold (2); The surface of the outer layer mold (2) is provided with holes; The sealing end caps (3) are mounted on both ends of the inner layer mold (1) and the outer layer mold (2), so that a closed cavity is formed inside the inner layer mold (1).

2. A silk protein gel shaping mold according to claim 1, characterized in that: The surface of the outer layer mold (2) is provided with openings at intervals along the circumferential direction.

3. A silk protein gel shaping mold according to claim 1, characterized in that: The surface of the outer layer mold (2) is provided with openings spaced apart in the axial direction.

4. A silk protein gel shaping mold according to claim 1, characterized in that: The surface openings of the outer layer mold (2) are array holes.

5. A silk protein gel shaping mold according to claim 1, characterized in that: The outer layer mold (2) and the inner layer mold (1) are arranged concentrically.

6. A silk protein gel shaping mold according to claim 1, characterized in that: The sealing end cover (3) is threadedly connected to the outer layer mold (2).

7. A silk protein gel shaping mold according to claim 1, characterized in that: The sealing end cover (3) is provided with a positioning slot, and the end of the inner layer mold (1) is inserted into the positioning slot to form a plug-in connection with the sealing end cover (3).

8. A silk protein gel shaping mold according to claim 7, characterized in that: A sealing member is arranged in the positioning slot.

9. A silk protein gel shaping mold according to claim 1, characterized in that: The sealing end cover (3) is provided with a positioning slot, and the end of the inner layer mold (1) is inserted into the positioning slot to form a snap connection with the sealing end cover (3).

10. A silk protein gel shaping mold according to claim 9, characterized in that: A sealing member is arranged in the positioning slot.

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