Multi-channel tubular photo-oxidation bioreactor device

Abstract

The utility model discloses a multichannel tubular photo-oxidation bioreactor device, which relates to the technical field of microalgae culture and online monitoring and comprises a water bath box, a gas regulation and control module, a dissolved oxygen monitoring module, an optical density monitoring module and a PH (potential of hydrogen) monitoring module. A glass culture tube and a temperature control module are mounted in the water bath box, the dissolved oxygen monitoring module extends into the glass culture tube through a probe or a sensor, and the optical density monitoring module is in contact with the glass culture tube through an optical sensor or a probe, so that accurate culture conditions are provided, and the microalgae culture efficiency is improved; according to the device, a controllable algae culture environment is provided, meanwhile, real-time monitoring of dissolved oxygen, OD value, PH and temperature is achieved, collection and analysis of scientific research data are facilitated, a user can conveniently set a culture program and check measurement data through a graphical interface of a control system, and the device is convenient to install and use due to the compact machine body structural design.

Description

Technical Field

[0001] The utility model relates to the technical field of microalgae cultivation and online monitoring, in particular to a multi-channel tubular photo-oxygen bioreactor device. Background Art

[0002] Microalgae photobioreactor is a device used to cultivate microalgae. It has wide application potential in many fields, including food, feed, biofuel production, etc. Microalgae, as an autotrophic organism, can use CO2 for photosynthesis, has the characteristics of fast growth rate and high biomass, and is a good raw material for producing energy biomass, food, feed or pharmaceutical industry. The design and application of microalgae photobioreactor needs to consider many factors, including light utilization efficiency, temperature control, online monitoring, etc.

[0003] The types of microalgae photooxygen bioreactors are mainly divided into two categories: open and closed. Open photobioreactors have a simple structure and low cost, and are suitable for large-scale cultivation, but may face problems such as pollution and difficulty in controlling culture conditions. Closed photooxygen bioreactors can provide better control conditions and are suitable for laboratories or small-scale cultivation, but are more expensive.

[0004] When designing a microalgae photo-oxygen bioreactor, key factors to consider include:

[0005] (1) Light utilization efficiency: Optimize photosynthesis conditions by increasing the specific surface area of ​​the reactor and designing different light paths;

[0006] (2) Temperature control: Microalgae growth is sensitive to temperature, and appropriate temperature control measures need to be taken to maintain a suitable growth environment;

[0007] (3) Online monitoring: The culture conditions can be adjusted in a timely manner by online monitoring of the biomass and photosynthesis of microalgae.

[0008] In a laboratory environment, traditional algae culture usually uses a reagent bottle, and the algae liquid in the reagent bottle is magnetically stirred in a temperature-controlled box. In order to determine the biomass content of the microalgae, it is necessary to take a sample from the reagent bottle and measure it using a spectrophotometer;

[0009] Traditional laboratory algae cultivation methods have the following problems:

[0010] (1) Light uniformity: Due to the large size of conventional laboratory reactors, it is difficult for light to penetrate all cells evenly, resulting in light absorption and scattering, which reduces the light intensity deep in the high-density microalgae solution. Cells cannot obtain the same intensity of light, affecting the efficiency of photosynthesis.

[0011] (2) Temperature control: The lack of precise water bath temperature control leads to temperature fluctuations in the microalgae growth environment, which may affect the growth rate of the microalgae;

[0012] (3) Biomass monitoring: It is necessary to use a spectrophotometer to measure the biomass concentration after sampling. This method is not only time-consuming, but also may interfere with the growth environment of microalgae due to the sampling process. Utility Model Content

[0013] 1. Technical issues to be resolved

[0014] In view of the deficiencies of the prior art, the utility model provides a multi-channel tubular photo-oxygen bioreactor device to solve the technical problems of poor illumination uniformity, lack of precise water bath temperature control, and inability to monitor biomass and photosynthesis in real time in the existing laboratory photo-oxygen bioreactor devices.

[0015] (II) Technical solution

[0016] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0017] A multi-channel tubular photo-oxygen bioreactor device comprises: a water bath, a gas control module, a dissolved oxygen monitoring module, an optical density monitoring module, and a pH monitoring module. A glass culture tube and a temperature control module are installed in the water bath. The dissolved oxygen monitoring module extends into the glass culture tube through a probe or a sensor, and the optical density monitoring module contacts the glass culture tube through an optical sensor or a probe.

[0018] Preferably: the temperature control module is located at the bottom of the water bath.

[0019] Preferably: a multi-channel intelligent LED light source is installed on the back of the water bath.

[0020] Preferably: a control module is installed on one side of the water bath.

[0021] Preferably: the multi-channel intelligent LED light source is connected to the control module via a circuit.

[0022] Preferably: the pH monitoring module is inserted into the culture solution in the glass culture tube through the pH electrode.

[0023] (III) Beneficial effects

[0024] The measuring device of the utility model provides accurate culture conditions (light, water temperature, gas) to improve the efficiency of microalgae culture. The device realizes real-time monitoring of dissolved oxygen, OD value, pH and temperature while providing a controllable culture environment for algae, which facilitates the collection and analysis of scientific research data. Through the graphical interface of the control system, users can easily set the culture program and view the measurement data. The compact body structure design is easy to install and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings.

[0026] Figure 1 It is the overall structure diagram of the utility model;

[0027] Figure 2 It is a side view of the overall structure diagram of the utility model.

[0028] Legend: 1. Water bath; 2. Glass culture tube; 3. Temperature control module; 4. Multi-channel intelligent LED light source; 5. Gas control module; 6. Dissolved oxygen monitoring module; 7. Optical density monitoring module; 8. PH monitoring module; 9. Control module. DETAILED DESCRIPTION

[0029] The embodiment of the present application provides a multi-channel tubular photo-oxygen bioreactor device, which effectively solves the technical problems of poor illumination uniformity, lack of precise water bath temperature control, and inability to monitor biomass and photosynthesis in real time in existing laboratory photo-oxygen bioreactor devices.

[0030] Example

[0031] The overall idea of ​​the technical solution in the embodiment of the present application is as follows:

[0032] In view of the problems existing in the prior art, the utility model provides a multi-channel tubular photo-oxygen bioreactor device, comprising a water bath 1, a glass culture tube 2, a temperature control module 3, a multi-channel intelligent LED light source 4, a gas control module 5, a dissolved oxygen monitoring module 6, an optical density monitoring module 7, a pH monitoring module 8, and a control module 9.

[0033] A plurality of (2, 4, 8) glass culture tubes 2 and a temperature control module 3 are installed in a water bath 1. The temperature control module 3 is located at the bottom of the water bath 1. The water temperature in the water bath 1 is adjusted and controlled by water bath heating and temperature sensor monitoring, thereby controlling the temperature of the algae solution in the glass culture tube 2. A multi-channel intelligent LED light source 4 is installed on the back of the water bath 1, and the light intensity and light cycle can be customized to provide the light required for the growth of microalgae. The gas control module 5 adds the gas in the external gas cylinder to each glass culture tube 2 through a gas mixing device. The probe-type dissolved oxygen sensor extends into the algae solution through the rubber plug of the culture tube, and the measuring instrument collects and records the dissolved oxygen content in real time to calculate the photosynthetic oxygen release and photosynthetic rate of the microalgae. The probe-type pH sensor extends into the algae solution through the rubber plug of the culture tube, and the measuring instrument collects and records the pH value of the algae solution in real time. The probe-type optical density sensor extends into the algae solution through the rubber plug of the culture tube, and measures the optical density of the microalgae at a specific wavelength during the culture process, thereby evaluating the biomass of the microalgae. The control module 9 is located at one side of the water bath 1, and sets the culture program through a graphical interface and displays a curve graph of measurement data online.

[0034] Working principle:

[0035] Step 1: inject the microalgae culture solution into each glass culture tube 2.

[0036] Step 2: Place the culture tube in the water bath 1.

[0037] Step three, using the water bath system to adjust the temperature of the water bath box 1.

[0038] Step 4: Start the LED lighting system to provide suitable lighting conditions for the microalgae.

[0039] Step 5: Optimize the gas-liquid mass transfer efficiency through the gas control module 5, and introduce the gas with the required composition and content into the culture tube.

[0040] Step six, the dissolved oxygen monitoring module 6 and the pH monitoring module 8 start to monitor the changes of dissolved oxygen and pH in the algae liquid in real time.

[0041] Step seven, the optical density monitoring module 7 regularly detects the change in the OD value of the algae liquid.

[0042] Step eight, setting the cultivation program through the control module 9 and displaying the measurement data online.

[0043] Step nine: Adjust the culture conditions based on the monitoring data to optimize the growth of microalgae.

[0044] Finally, it should be noted that: Obviously, the above embodiments are only examples for clearly explaining the present invention, and are not intended to limit the implementation methods. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from this are still within the scope of protection of the present invention.

Claims

1. A multi-channel tubular photo-oxygen bioreactor device, characterized in that: The multi-channel tubular photo-oxygen bioreactor device comprises: a water bath (1), a gas control module (5), a dissolved oxygen monitoring module (6), an optical density monitoring module (7), and a pH monitoring module (8); A glass culture tube (2) and a temperature control module (3) are installed in the water bath (1); a dissolved oxygen monitoring module (6) extends into the glass culture tube (2) via a probe or a sensor; and an optical density monitoring module (7) contacts the glass culture tube (2) via an optical sensor or a probe.

2. A multi-channel tubular photo-oxygen bioreactor device as claimed in claim 1, characterized in that: The temperature control module (3) is located at the bottom of the water bath (1).

3. A multi-channel tubular photo-oxygen bioreactor device as claimed in claim 1, characterized in that: A multi-channel intelligent LED light source (4) is installed on the back of the water bath (1).

4. A multi-channel tubular photo-oxygen bioreactor device as claimed in claim 3, characterized in that: A control module (9) is installed on one side of the water bath (1).

5. A multi-channel tubular photo-oxygen bioreactor device as claimed in claim 4, characterized in that: The multi-channel intelligent LED light source (4) is connected to the control module (9) via a circuit.

6. A multi-channel tubular photo-oxygen bioreactor device as claimed in claim 2, characterized in that: The pH monitoring module (8) is inserted into the culture solution in the glass culture tube (2) through the pH electrode.

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