Centrifugal dyeing sheet sealing device

Abstract

The utility model relates to the technical field of cell smears, in particular to a centrifugal staining and sealing device, which is characterized in that a cell smear device is placed in the centrifugal staining device for centrifuging and staining operation, and after centrifuging and staining are completed, the stained cell smear device is sequentially subjected to disassembly, glue dripping and cover glass sealing by the sealing device. The traditional manual operation is replaced, so that the burden of the manual operation is reduced, the sample can be correctly separated and sealed, and standard cell slide preparation is realized.

Description

Technical Field

[0001] The utility model relates to the technical field of cell smears, and more specifically, to a centrifugal staining and sealing device. Background Art

[0002] Manual centrifugal mounting is a laboratory procedure used to separate cells or substances from biological samples (such as blood, cell suspensions, etc.) and mount them on slides for further microscopic observation or analysis.

[0003] Manual centrifugation of coverslips requires a lot of time and effort. Operators need to manually place the tubes into the centrifuge and wait for the centrifugation process to complete. This can increase the workload, especially when processing large batches of samples.

[0004] Manual operation of the centrifuge requires accurate control of the speed and time to ensure that the samples can be separated and sealed correctly. This places high technical requirements on the operator, especially for novices, who may need some learning and training. Utility Model Content

[0005] In view of the above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a centrifugal staining and sealing device, which has the advantages of replacing traditional manual work, reducing the burden of manual work, and ensuring that samples can be correctly separated and sealed.

[0006] The above technical purpose of the utility model is achieved through the following technical solutions: a centrifugal staining and sealing device, comprising a workbench, on which is arranged:

[0007] A centrifugal staining device, used for centrifugal separation and staining of a cell smear;

[0008] The sealing device is used to dismantle the cell smear after staining the centrifugal device, drip glue and seal the slide with a cover glass in sequence.

[0009] Preferably, the centrifugal device comprises a centrifuge arranged on a workbench and a staining structure operating above the centrifuge, and the staining structure stains the cell smear during the interval time when the centrifuge is operating.

[0010] Preferably, the staining structure includes a support frame, a multi-axis robotic arm mounted on the support frame, a support table arranged at the end of the multi-axis robotic arm, and a staining outflow mechanism installed on the support table for selectively selecting staining droplets for cell smears. The staining outflow mechanism is connected to an integrated confluence mechanism for integrating and converging a variety of staining liquids. A control module for controlling the cutoff or establishing a connection between the staining outflow mechanism and the integrated confluence mechanism is arranged between the staining outflow mechanism and the integrated confluence mechanism. The support table is driven away from and close to the centrifuge by the multi-axis robotic arm, and combined with the control of the control module, the cell sample in the cell smear can be selectively stained by droplets.

[0011] Preferably, the dye outflow mechanism includes a plurality of dye tubes connected to the integrated confluence mechanism, and each dye tube corresponds to a different dye solution;

[0012] It also includes a plurality of negative pressure pumps installed on the support platform, each of the negative pressure pumps is connected to a corresponding pipette, and the pipettes correspond to the cell smear devices one by one.

[0013] Preferably, the integrated confluence mechanism includes a confluence plate, a liquid inlet channel opened in the confluence plate for the dye liquid to enter, and a liquid outlet channel opened in the confluence plate for the dye liquid to flow out, and one of the liquid inlet channels is connected to one of the liquid outlet channels.

[0014] Preferably, a confluence channel is provided in the confluence plate, and the liquid inlet channel for the same dyeing liquid to flow in is connected through the confluence channel, and the control module controls the communication between the liquid inlet channel and the confluence channel.

[0015] Preferably, the control module includes an electrical control box and a plurality of solenoid valves electrically connected to the electrical control box, wherein the solenoid valves are installed on the integrated confluence mechanism, and the switches of the solenoid valves are controlled by the electrical control box to cut off or establish communication between the dye outflow mechanism and the integrated confluence mechanism.

[0016] Preferably, the sealing device comprises a gantry bracket mounted on a workbench and a placement fixture sliding on the workbench, wherein the gantry bracket is provided with a grabbing component for grabbing a cover glass, a glue dripping component for dripping glue on a cell smear apparatus, and a removal component for removing a centrifuge tube on a cell smear apparatus;

[0017] Cooperating with the sliding of the placement fixture, the centrifuge tube on the cell smear apparatus is removed in turn through the removal component, the slide on the cell smear apparatus is glued through the glue dripping component, and the cover glass is grabbed by the grabbing component and covered on the slide to complete the sealing operation.

[0018] Preferably, the grabbing assembly includes a suction claw and a first driving member driving the suction claw to approach and move away from the workbench; the glue dripping assembly includes an injector, a second driving member driving the injector to drip liquid, and a third driving member driving the injector to approach and move away from the workbench; the removal assembly includes a rotating jaw and a fourth driving member driving the rotating jaw to approach and move away from the workbench.

[0019] Preferably, a placement station for placing the cell smear is provided on the workbench, and the cell smear is grabbed by the grabbing component or the removal component and moved from the placement fixture to the placement station.

[0020] In summary, the utility model has the beneficial effects of placing the cell smear into the centrifugal staining device to perform centrifugation and staining operations, and after the centrifugation and staining are completed, using the sealing device to sequentially remove the stained cell smear, drip glue, and seal with a cover glass, replacing traditional manual operations and reducing the burden of manual operations to ensure that the sample can be correctly separated and sealed, thereby achieving standardized cell preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the utility model;

[0022] Figure 2 It is a partial schematic diagram of the dyeing outflow mechanism of the embodiment of the utility model;

[0023] Figure 3 It is a cross-sectional view of a busbar according to an embodiment of the utility model.

[0024] Figure numerals: 1. workbench; 11. centrifuge; 12. support frame; 13. multi-axis robot arm; 14. support table; 15. dyeing outflow mechanism; 151. dyeing tube; 152. negative pressure pump; 153. pipette; 16. integrated confluence mechanism; 161. confluence plate; 162. liquid inlet channel; 163. liquid outlet channel; 164. confluence channel; 17. electric control box; 18. solenoid valve; 2. gantry bracket; 21. placement fixture; 22. grabbing assembly; 23. glue dripping assembly; 24. removal assembly; 25. linear module; 3. placement station. DETAILED DESCRIPTION

[0025] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0026] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it can be directly on the other component or indirectly on the other component. When a component is referred to as being "connected to" another component, it can be directly or indirectly connected to the other component.

[0027] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0028] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0029] A centrifugal staining and sealing device, see Figures 1 to 3 , comprising a workbench 1, on which is disposed:

[0030] A centrifugal staining device, used for centrifugal separation and staining of a cell smear;

[0031] The sealing device is used to dismantle the cell smear after staining the centrifugal device, drip glue and seal the slide with a cover glass in sequence.

[0032] In this embodiment, the cell smear is placed in a centrifugal staining device to perform centrifugation and staining operations. After the centrifugation and staining are completed, the stained cell smear is removed, glue is dripped, and coverslips are used to seal the slides in sequence using a sealing device, replacing traditional manual operations and reducing the burden of manual operations to ensure that the sample can be correctly separated and sealed, thereby achieving standardized cell preparation.

[0033] Specifically, the centrifugal device includes a centrifuge 11 disposed on a workbench 1 and a staining structure operating above the centrifuge 11 , and the staining structure stains the cell smear during the interval time when the centrifuge 11 is operating.

[0034] The centrifuge 11 centrifuges the cell smear. During the interval of the centrifuge 11, the staining structure intervenes to inject the corresponding stain into the cell smear in the centrifuge 11, thereby completing the double process of centrifugation and staining.

[0035] Specifically, the staining structure includes a support frame 12, a multi-axis robotic arm 13 installed on the support frame 12, a support platform 14 arranged at the end of the multi-axis robotic arm 13, and a staining outflow mechanism 15 installed on the support platform 14 for selectively selecting staining droplets for cell smears. The staining outflow mechanism 15 is connected to an integrated confluence mechanism 16 for integrating and converging multiple staining liquids. A control module for controlling the cutoff or establishing a connection relationship between the staining outflow mechanism 15 and the integrated confluence mechanism 16 is arranged between the staining outflow mechanism 15 and the integrated confluence mechanism 16. The support platform 14 is driven away from and close to the centrifuge 11 by the multi-axis robotic arm 13, and combined with the control of the control module, the cell sample in the cell smear can be selectively stained by droplets.

[0036] The staining process is carried out by connecting the dyeing liquid to the integrated confluence mechanism 16, and converging various dyeing liquids in the integrated confluence mechanism 16 through a water pump, cooperating with the multi-axis robotic arm 13 to drive the support platform 14 away from and close to the centrifuge 11, and under the control of the control module, utilizing the cutoff or connection relationship between the dyeing outflow mechanism 15 and the integrated confluence mechanism 16 to selectively perform drop staining on the cell sample in the cell smear.

[0037] Specifically, the integrated confluence mechanism 16 includes a confluence plate 161 , a liquid inlet channel 162 provided in the confluence plate 161 for the dye liquid to enter, and a liquid outlet channel 163 provided in the confluence plate 161 for the dye liquid to flow out, and one liquid inlet channel 162 is connected to one liquid outlet channel 163 .

[0038] In this embodiment, twelve liquid inlet channels 162 and twelve liquid outlet channels 163 are opened in the confluence plate 161. Each liquid inlet channel 162 and each liquid outlet channel 163 are independently arranged, and one liquid inlet channel 162 is connected to one liquid outlet channel 163, and then the liquid outlet channel 163 is connected to one dyeing tube 151, so as to avoid mutual interference between dyeing liquids and realize the circulation and transportation of dyeing liquids.

[0039] The integrated form of the manifold 161 avoids the connection arrangement of traditional pipelines and the trouble of densely arranging multiple lines.

[0040] Specifically, the dye outflow mechanism 15 includes a plurality of dye tubes 151 connected to the integrated confluence mechanism 16, and each dye tube 151 corresponds to a different dye solution.

[0041] It also includes a plurality of negative pressure pumps 152 installed on the support platform 14 , each negative pressure pump 152 is connected to a corresponding pipette 153 , and the pipette 153 corresponds to the cell smear device one by one.

[0042] In this embodiment, six dyeing tubes 151 are arranged in a set of dyeing outflow mechanisms 15. Each dyeing tube 151 corresponds to a dyeing liquid. The required dyeing liquid can be added autonomously according to the dyeing requirements.

[0043] Since the cell fluid is processed after centrifugation before staining, a layer of impurities floats on the surface of the cell fluid in the centrifuge tube. It is necessary to suck it away to avoid affecting the subsequent staining.

[0044] The pipette 153 of this embodiment is bound together with the dyeing tube 151 and moves with the dyeing tube 151. If dyeing needs to be carried out through the dyeing tube 151, the pipette 153 must be used to absorb waste liquid first, and negative pressure is generated in the pipette 153 through the negative pressure pump 152. Each group of dyeing outflow mechanisms 15 corresponds to a group of pipettes 153.

[0045] Specifically, the control module includes an electrical control box 17 and a plurality of solenoid valves 18 electrically connected to the electrical control box 17 . The solenoid valves 18 are installed on the integrated confluence mechanism 16 . The switches of the solenoid valves 18 are controlled by the electrical control box 17 to cut off or establish communication between the dyeing outflow mechanism 15 and the integrated confluence mechanism 16 .

[0046] In this embodiment, an electric control box 17 controls the solenoid valve 18, and the solenoid valve 18 is used to control the connection between the confluence channel 164 and each liquid inlet channel 162, so as to realize the control of the type selection and directional distribution of the dyeing liquid.

[0047] Specifically, the sealing device includes a gantry support 2 installed on a workbench 1 and a placement fixture 21 sliding on the workbench 1, and the gantry support 2 is provided with a grabbing component 22 for grabbing a cover glass, a glue dripping component 23 for dripping glue on a cell smear, and a removal component 24 for removing a centrifuge tube on a cell smear;

[0048] Cooperating with the sliding of the placement fixture 21, the centrifuge tube on the cell smear apparatus is removed in turn through the removal component 24, the glass slide on the cell smear apparatus is glued through the glue dripping component 23, and the cover glass is grabbed by the grabbing component 22 and covered on the glass slide to complete the sealing operation.

[0049] After centrifugation and staining, the coverslipping process will begin.

[0050] The cell smear devices are placed in the placement fixture 21 in order, and with the sliding of the placement fixture 21, the cell smear devices go through the removal component 24, the glue dripping component 23 and the grabbing component 22 in order to complete the slide sealing operation.

[0051] Specifically, the grabbing assembly 22 includes a suction claw and a first driving member that drives the suction claw to approach and move away from the workbench 1; the glue dripping assembly 23 includes an injector, a second driving member that pushes the injector to drip liquid, and a third driving member that drives the injector to approach and move away from the workbench 1; the removal assembly 24 includes a rotating jaw and a fourth driving member that drives the rotating jaw to approach and move away from the workbench 1.

[0052] The first driving member, the third driving member and the fourth driving member are lifting cylinders, and the second driving member is a propulsion cylinder. The lifting cylinders are used to lift and lower the height of the injector, the suction claw and the rotating clamp, and the injector is controlled by the pushing cylinder to perform a dripping operation.

[0053] In this embodiment, a linear module 25 is provided on the workbench 1 , and the placement fixture 21 is driven to move by the linear module 25 .

[0054] Specifically, a placement station 3 for placing a cell smear is provided on the workbench 1 , and the cell smear is grabbed by the grabbing component 22 or the removing component 24 and moved from the placement fixture 21 to the placement station 3 .

[0055] The sealing device of this embodiment can not only be used for sealing operations, but also for transporting cell smears. The grabbing component 22 or the removal component 24 grabs the cell smear from the placement fixture 21 to the placement station 3, and cooperates with an external manipulator or manual labor to transfer the cell smear that needs centrifugal staining to the centrifugal staining device to complete the transportation operation, which has the function of one thing for multiple uses.

[0056] The above embodiments are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A centrifugal staining and sealing device, characterized in that: A workbench is provided, on which: A centrifugal staining device, used for centrifugal separation and staining of a cell smear; The sealing device is used to dismantle the cell smear after staining the centrifugal device, drip glue and seal the slide with a cover glass in sequence.

2. A centrifugal staining and sealing device according to claim 1, characterized in that: The centrifugal device comprises a centrifuge arranged on a workbench and a staining structure operating above the centrifuge, and the staining structure stains the cell smear during the interval time when the centrifuge stays in operation.

3. A centrifugal staining and sealing device according to claim 2, characterized in that: The staining structure includes a support frame, a multi-axis robotic arm installed on the support frame, a support table arranged at the end of the multi-axis robotic arm, and a staining outflow mechanism installed on the support table for selectively selecting staining drops for cell smears. The staining outflow mechanism is connected to an integrated confluence mechanism for integrating and converging multiple staining liquids. A control module for controlling the cutoff or establishing a connection relationship between the staining outflow mechanism and the integrated confluence mechanism is arranged between the staining outflow mechanism and the integrated confluence mechanism. The multi-axis robotic arm drives the support table away from and close to the centrifuge, and combined with the control of the control module, the cell sample in the cell smear can be selectively stained by droplets.

4. A centrifugal staining and sealing device according to claim 3, characterized in that: The dye outflow mechanism includes a plurality of dye tubes connected to the integrated confluence mechanism, and each dye tube corresponds to a different dye solution; It also includes a plurality of negative pressure pumps installed on the support platform, each of the negative pressure pumps is connected to a corresponding pipette, and the pipettes correspond to the cell smear devices one by one.

5. A centrifugal staining and sealing device according to claim 4, characterized in that: The integrated confluence mechanism includes a confluence plate, a liquid inlet channel provided in the confluence plate for the dye liquid to enter, and a liquid outlet channel provided in the confluence plate for the dye liquid to flow out, and one of the liquid inlet channels is connected to one of the liquid outlet channels.

6. A centrifugal staining and sealing device according to claim 5, characterized in that: The confluence plate is provided with a confluence channel, and the liquid inlet channel for the same dyeing liquid to flow in is connected through the confluence channel, and the control module controls the communication between the liquid inlet channel and the confluence channel.

7. A centrifugal staining and sealing device according to claim 3, characterized in that: The control module includes an electrical control box and a plurality of solenoid valves electrically connected to the electrical control box. The solenoid valves are installed on the integrated confluence mechanism. The switches of the solenoid valves are controlled by the electrical control box to cut off or establish communication between the dye outflow mechanism and the integrated confluence mechanism.

8. A centrifugal staining and sealing device according to claim 1, characterized in that: The sealing device comprises a gantry support mounted on a workbench and a placement fixture sliding on the workbench, wherein the gantry support is provided with a grabbing component for grabbing a cover glass, a glue dripping component for dripping glue on a cell smear device, and a removal component for removing a centrifuge tube on a cell smear device; Cooperating with the sliding of the placement fixture, the centrifuge tube on the cell smear apparatus is removed in turn through the removal component, the slide on the cell smear apparatus is glued through the glue dripping component, and the cover glass is grabbed by the grabbing component and covered on the slide to complete the sealing operation.

9. A centrifugal staining and sealing device according to claim 8, characterized in that: The grabbing assembly includes a suction claw and a first driving member driving the suction claw to approach and move away from the workbench; the glue dripping assembly includes an injector, a second driving member driving the injector to drip liquid, and a third driving member driving the injector to approach and move away from the workbench; the removal assembly includes a rotating jaw and a fourth driving member driving the rotating jaw to approach and move away from the workbench.

10. A centrifugal staining and sealing device according to claim 8, characterized in that: The workbench is provided with a placement station for placing the cell smear device, and the cell smear device is grabbed by the grabbing component or the removal component and moved from the placement fixture to the placement station.

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