Microalgae hydrogel particle in-situ treatment device for nitrogen and phosphorus removal of bay water body

Abstract

The utility model relates to the technical field of deep nitrogen and phosphorus removal of a water body, in particular to an in-situ treatment device suitable for a bay water body denutrition process, which comprises a support device and a microalgae hydrogel particle bearing device, the supporting frame is used for supporting and fixing the microalgae hydrogel particle bearing device and is fixedly connected with the floating cylinder, and the floating cylinder is fixedly connected with the vertically arranged heavy object so as to realize the positioning of the height in water. According to the water eutrophication in-situ treatment device provided by the utility model, biomass fixation and in-situ nitrogen and phosphorus removal are carried out by arranging the capsule body filled with the microalgae hydrogel particles, and the microalgae hydrogel particles are used as a buffer, so that microalgae biomass grows in the hydrogel, the biomass can be effectively enriched under a certain stress environment condition, and the water eutrophication in-situ treatment device has the advantages that the water eutrophication in-situ treatment effect is improved; the problems that biomass of a traditional biological membrane is lost and the integrity of a cell membrane is damaged due to the influence of hydraulic shearing force are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of deep denitrification and phosphorus removal of water bodies, in particular to an in-situ treatment device suitable for the de-nutrition process of bay water bodies. Background Art

[0002] Eutrophication of water bodies in the Bay is one of the water environment problems that need to be solved urgently. Eutrophication of water bodies is one of the main causes of algae blooms, which reduces the dissolved oxygen level in water bodies, leads to water quality deterioration, and causes harm to the aquatic environment ecosystem. Microalgae can absorb nitrogen, phosphorus, inorganic carbon, dissolved organic carbon, etc. through photoautotrophic and polyculture processes. Microalgae biomass can realize the transformation and accumulation of organic products in cells, and can also participate in the thermal utilization process as biomass to achieve denitrification and phosphorus removal and biomass resource utilization. Therefore, more and more research and pilot-scale applications focus on the cultivation of microalgae, water treatment based on microalgae, and biological carbon fixation processes. Some studies have proposed the use of microalgae to achieve extreme denitrification and phosphorus removal technology for eutrophic water bodies. However, most of the current water treatment and biological carbon fixation technologies based on microalgae are realized by using process processes with bioreactors as the core, which are not suitable for the eutrophication reduction treatment of bay water bodies. It is necessary to develop in-situ treatment devices for denitrification and phosphorus removal in bay water bodies for deep reduction technology of microalgae eutrophication.

[0003] However, the in-situ treatment technology for reducing eutrophication of water bodies with microalgae as the core faces the following problems: 1. Biomass recovery; 2. Biological pollution; 3. Biomass loss. At present, the in-situ treatment method based on microalgae is mainly based on biofilm. Although biomass enrichment and recovery can be achieved, low nutrient concentration and biomass pollution will limit the stable formation of microalgae biofilm. Hydraulic shear force will cause biomass loss and destroy the integrity of cell membranes, leading to the release of intracellular metabolites, which will increase the TOC concentration of environmental water bodies. Therefore, the continuous operation capacity and treatment effect of biofilm in-situ treatment are limited. Utility Model Content

[0004] In order to solve the main problems of the existing in-situ denitrification and phosphorus removal technology based on microalgae and develop an in-situ treatment technology for biological denitrification and phosphorus removal suitable for bay water bodies, the utility model proposes an in-situ treatment device for microalgae hydrogel particles suitable for deep denitrification and phosphorus removal in bay water bodies.

[0005] The technical problem of the utility model is solved by the following technical solutions:

[0006] A microalgae hydrogel particle in-situ treatment device suitable for nitrogen and phosphorus removal in bay water bodies, comprising a supporting device and a microalgae hydrogel particle carrying device, wherein the supporting device comprises a supporting frame and a buoy, the supporting frame is used to support and fix the microalgae hydrogel particle carrying device and is fixedly connected to the buoy, and the buoy is fixedly connected to a vertically arranged weight to achieve height positioning in the water.

[0007] Furthermore, the buoy is filled with gas, and the amount of gas in the buoy is adjusted so that the microalgae hydrogel particle carrying device is submerged below the surface of the water body.

[0008] Furthermore, the microalgae hydrogel particle carrying device comprises a plurality of microalgae hydrogel particle carrying capsules, and two adjacent carrying capsules are connected via a connecting flange, and the main body of the carrying capsule is perpendicular to the water flow direction.

[0009] Furthermore, the outer surface of the microalgae hydrogel particle carrying capsule is provided with a selective permeable membrane and is surrounded by the membrane to form a main body.

[0010] Furthermore, a sodium alginate-microalgae-activated carbon powder composition is arranged inside the microalgae hydrogel particle carrier capsule.

[0011] Furthermore, the activated carbon particle size in the sodium alginate-microalgae-activated carbon powder composition is less than 0.04 mm.

[0012] Furthermore, the microalgae in the sodium alginate-microalgae-activated carbon powder composition is Chlorella, Scenedesmus, Mononemus, Microcystis aeruginosa or a combination thereof.

[0013] Furthermore, the concentration of microalgae in the microalgae hydrogel particle loading capsule is 5000-8000 / L.

[0014] The beneficial effects of the utility model compared with the prior art include:

[0015] The in-situ treatment device for eutrophication of water bodies proposed by the utility model fixes biomass and removes nitrogen and phosphorus in situ by setting a capsule filled with microalgae hydrogel particles inside. The microalgae hydrogel particles are used as a buffer to allow the microalgae biomass to grow in the hydrogel body, and can effectively enrich the biomass under certain stress environmental conditions, avoiding the loss of biomass in traditional biofilms and preventing the problem of cell membrane integrity being destroyed due to hydraulic shear force, and can maintain a continuous and stable biomass concentration during the treatment process, reducing the degree of biological pollution in the real environment water body; the capsule can be adjusted by the float to be just below the water surface, reducing the growth inhibition of microalgae biomass by light attenuation; at the same time, the utility model performs in-situ treatment through the in-situ treatment device for eutrophication of water bodies, avoiding the location restriction of the bioreactor and reducing the construction, operation and maintenance costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the utility model.

[0017] Figure 1 It is the microalgae hydrogel particle in-situ treatment device for deep denitrification and phosphorus removal of bay water body of the present application: 1 support frame 2 buoy 3 microalgae hydrogel particle carrying capsule;

[0018] Figure 2 This is a front view of the microalgae hydrogel particle in-situ treatment device for deep denitrification and phosphorus removal in the bay water of the present application;

[0019] Figure 3 A three-dimensional diagram of the in-situ processing device for microalgae hydrogel particles;

[0020] Figure 4 It is a front view of the microalgae hydrogel particle carrying capsule;

[0021] Figure 5 is a top view of a microalgae hydrogel particle-carrying capsule;

[0022] Figure 6 A three-dimensional image of a microalgae hydrogel particle-carrying capsule. DETAILED DESCRIPTION

[0023] The following is a further detailed description of the microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay waters proposed by the utility model in combination with the accompanying drawings and specific embodiments. It should be noted that the accompanying drawings are in a very simplified form and are not in precise proportions, and are only used to conveniently and clearly assist in explaining the purpose of the embodiments of the utility model. The same or similar reference numerals in the accompanying drawings represent the same or similar components.

[0024] The present application provides an in-situ treatment device for microalgae hydrogel particles suitable for deep denitrification and phosphorus removal in bay waters, including a support device and a microalgae hydrogel particle carrying device. Figures 1 to 3 As shown in the figure, the support device includes a support frame 1 for binding the microalgae hydrogel particle carrying capsule 3 and fixedly connected to a buoy 2, and the buoy 2 is connected to a fixed weight arranged in the direction to be fixed in the water area to be treated (see Figure 2 ), by adjusting the weight and the amount of air in the float, the water surface is just submerged on the upper surface of the microalgae hydrogel particle carrying capsule 3. The microalgae hydrogel particle carrying device is mainly composed of the microalgae hydrogel particle carrying capsule 3 ( Figure 1 and Figure 2 ).like Figure 5As shown, every two capsules 5 of the microalgae hydrogel particle carrying capsule 3 are connected by a connecting flange 4, and the capsule 5 is perpendicular to the water flow direction. The microalgae hydrogel particles are added / recovered into the capsule through the feed / discharge port 6. Figure 6 As shown, the outer surface of the capsule 5 is provided with a selective permeable membrane and is surrounded by it (forward osmosis).

[0025] After adjustment by the float and the weight, the microalgae hydrogel particle carrying capsule connected to the support frame (1) should be just below the water surface, which can reduce the inhibitory effect of light attenuation on the specific growth rate of microalgae in the hydrogel particles. The arrangement of the microalgae hydrogel particle capsule should be perpendicular to the water flow direction (such as Figure 2 As shown in ( ), because the capsule is made of a selective permeable membrane, the forward osmosis principle can be used to increase the concentration of the nutrient salt base in the capsule (5) and use the microalgae biomass in the hydrogel particles for assimilation and absorption. At the same time, the hydrogel particles can avoid the loss of biomass in the process of assimilation and absorption of nitrogen and phosphorus, reduce the impact of salinity on microalgae biomass, and alleviate the damage of hydraulic shear force in the in-situ water body to the microalgae cell membrane. After running for 3-4 days, the microalgae hydrogel particles can be removed by replacing the microalgae hydrogel carrier capsule (3), and the biological products accumulated in the recovered microalgae biomass can realize the resource utilization of biomass. This process can produce biomass in a relatively short period of time and realize the in-situ denitrification and phosphorus removal of the bay water body through microalgae biomass.

[0026] During specific use, the effective working volume of a single microalgae hydrogel particle carrier capsule is 5-20L (adjustable according to processing requirements), the ratio of microalgae hydrogel particles to capsule operating volume should be 5000-8000 / L, and the microalgae used can be green algae and cyanobacteria with high nitrogen and phosphorus assimilation and absorption rates (such as Chlorella, Scenedesmus, Mononema, Microcystis, etc.).

[0027] Specifically, the preparation method of the microalgae hydrogel particles in the utility model includes:

[0028] S1: Add a microalgae solution with a certain algae cell concentration (preferably 10 3 -10 7 / mL, selecting a suitable starting concentration range can effectively promote growth and material absorption rate; the microalgae species are preferably algae species such as cyanobacteria and green algae that can stably assimilate and absorb nitrogen elements), centrifuge and concentrate at 4000-10000rpm for 5-10 minutes, remove the supernatant (which can effectively remove impurities, bacteria and organic matter), and resuspend with physiological saline, phosphate-buffered saline (PBS) or deionized water (algae liquid is obtained by resuspension) to obtain a microalgae stock solution (algae liquid).

[0029] S2: The above-mentioned microalgae stock solution (algae solution) is mixed with a certain concentration of sodium alginate solution (the concentration is preferably 1%-5%, which can ensure the stability of the hydrogel ball and the good growth of the microalgae) in a ratio of 1:1 (v / v) (that is, the same volume of two concentrations are mixed together at a ratio of 1:1), and activated carbon powder is added in a ratio of 1:20-1:10 (w / w) to the microalgae biomass (that is, the mass of the added activated carbon powder is one-tenth to one-twentieth of the dry weight of the microalgae biomass, which enhances the adsorption effect by adding activated carbon powder, improves the adsorption capacity of the surface of the microalgae particles for smaller particle sizes and difficult to melt substances, and ensures the surface transmittance of the hydrogel ball). (The particle size of the activated carbon powder needs to be less than a certain value, and its particle size will affect the particle structure and transmittance. Preferably, the particle size of the powdered activated carbon is <0.04mm), and fully mixed for 2-3h to form a sodium alginate-algae solution-activated carbon powder mixed solution, and then the sodium alginate-algae solution-activated carbon powder mixed solution is poured into a syringe pump (using an electric or pneumatic method). .

[0030] S3: The mixed solution (i.e., sodium alginate-algae liquid-activated carbon powder mixed solution) in the above-mentioned injection pump is dripped at a uniform speed (preferably at a rate of 0.05-0.08 mm / s to push the mixed solution at a uniform speed) into a calcium chloride solution of a predetermined concentration (the predetermined concentration must be guaranteed to be a suitable concentration for the cross-linking reaction, preferably 1%-5%), and uniform stirring is maintained during the period. The sodium alginate-algae liquid-activated carbon powder mixed solution and calcium chloride undergo a cross-linking reaction to form hydrogel microalgae particles, wherein the volume of the calcium chloride solution should be greater than the volume of the mixed solution, and a certain depth should be ensured to prevent the newly formed hydrogel balls from adhering to the wall, colliding with each other, and adhering; let it stand The immobilization reaction is fixed for 2-3 hours; the microalgae hydrogel particles prepared by selecting different injection needle calibers (the injection needle caliber is preferably 0.4-1.4 mm) (the mixed solution of algae liquid-sodium alginate-activated carbon powder is added dropwise to the calcium chloride solution by pneumatic or electric injection, and the particle size of the microalgae hydrogel particles can be controlled by pushing and the aperture of the injection head, and the diameter range of the microalgae hydrogel particles is preferably 2-5 mm), and finally the prepared microalgae hydrogel particles are washed 2-3 times with clean water (tap water / ultrapure water / distilled water can be selected) to wash the preparation solution remaining on the surface.

[0031] The practical use of the embodiment of the utility model is described below through specific implementation steps:

[0032] A. A microalgae solution with an algae cell dry weight of about 200 mg / L (the microalgae species can be (cyanobacteria, green algae, etc.) that can stably assimilate and absorb nitrogen) is centrifuged and concentrated at 8000 rpm for 5 minutes, the supernatant is removed, and the solution is resuspended with physiological saline / PBS / deionized water to obtain a microalgae stock solution (algae solution).

[0033] B. Mix the above algae solution with a 3% sodium alginate solution at a ratio of 1:1 (v / v), add powdered activated carbon (particle size <0.04mm) at a ratio of 1:10 (w / w) to microalgae biomass, mix thoroughly for 2h, and then pour the sodium alginate-algae solution-activated carbon powder mixed solution into a syringe pump (electric or pneumatic).

[0034] C. The mixed liquid in the above injection pump is dripped into a 4% calcium chloride solution at a uniform speed (0.06mm / s), and uniform stirring is maintained during the process. The algae liquid-sodium alginate mixed liquid and calcium chloride undergo a cross-linking reaction to form hydrogel microalgae particles. The volume of the calcium chloride solution should be appropriately larger than the volume of the mixed liquid, and a certain depth should be ensured to avoid the newly formed hydrogel balls from adhering to the wall, colliding with each other and adhering to each other. Let it stand and fix for 2 hours for the immobilization reaction. Microalgae hydrogel particles with a particle diameter range of 2-3mm are prepared by selecting a 0.4mm caliber injection, and finally the prepared microalgae hydrogel particles are washed 2-3 times with ultrapure water.

[0035] D. Pour the prepared microalgae hydrogel particles into the microalgae hydrogel particle carrier capsule, 5000-8000 particles / L.

[0036] E. According to the treatment requirements, a number of microalgae hydrogel particle carrying capsules are fixed on the support frame (1), and the buoys and weights are adjusted to ensure that the microalgae hydrogel particle carrying capsules are just below the water surface and perpendicular to the water flow direction.

[0037] F. The growth period of the microalgae hydrogel particles is 2-4 days. When the biomass reaches a peak value, the microalgae biomass hydrogel particles are recovered by recycling and replacing the microalgae hydrogel particle carrier capsule.

[0038] The above description is only a description of the preferred embodiment of the utility model, and is not any limitation on the scope of the utility model. Any changes and modifications made by ordinary technicians in the field of the utility model based on the above disclosure shall fall within the scope of protection of the claims.

Claims

1. An in-situ treatment device for microalgae hydrogel particles for denitrification and phosphorus removal in bay waters, characterized in that: It comprises a supporting device and a microalgae hydrogel particle carrying device, wherein the supporting device comprises a supporting frame and a buoy, the supporting frame is used to support and fix the microalgae hydrogel particle carrying device and is fixedly connected to the buoy, and the buoy is fixedly connected to a vertically arranged weight to achieve height positioning in water.

2. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 1 is characterized in that: The buoy is filled with gas, and the microalgae hydrogel particle carrying device is immersed below the surface of the water body by adjusting the amount of gas in the buoy.

3. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 1 is characterized in that: The microalgae hydrogel particle carrying device comprises a plurality of microalgae hydrogel particle carrying capsules, and two adjacent carrying capsules are connected via a connecting flange, and the main body of the carrying capsule is perpendicular to the water flow direction.

4. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 3 is characterized in that: The outer surface of the microalgae hydrogel particle carrying capsule is provided with a selective permeable membrane and is surrounded by the membrane to form a main body.

5. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 3 is characterized in that: A sodium alginate-microalgae-activated carbon powder composition is arranged inside the microalgae hydrogel particle carrying capsule.

6. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 5, characterized in that: The activated carbon particle size in the sodium alginate-microalgae-activated carbon powder composition is less than 0.04 mm.

7. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 5, characterized in that: The microalgae in the sodium alginate-microalgae-activated carbon powder composition are Chlorella, Scenedesmus, Mononema, Microcystis aeruginosa or a combination thereof.

8. The microalgae hydrogel particle in-situ treatment device for denitrification and phosphorus removal in bay water according to claim 7, characterized in that: The microalgae concentration in the microalgae hydrogel particle carrier capsule is 5000-8000 / L.

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