Cell harvesting scaffold for autophagy

Abstract

The utility model relates to the field of cell culture, and discloses a cell harvesting support for cell autophagy, which comprises a bottom plate, the upper part of the top surface of the bottom plate is fixedly connected with a support body, the upper part of the support body is provided with a placing plate, the right side of the support body is fixedly connected with a motor, and the rear side of the placing plate is provided with a hook piece. A fixing piece is arranged on the front side of the containing plate, an auxiliary piece is arranged on the upper portion of the containing plate, vertical plates are fixedly connected to the left side and the right side of the top face of the containing plate, connecting grooves are formed in the left portion and the right portion of the front side of the top face of the containing plate, a sliding groove is formed in the middle of the rear side of the top face of the containing plate, and sliding openings are formed in the upper portions of the vertical plates. And the output end of the motor is fixedly connected with a rotating shaft. According to the cell culture device disclosed by the utility model, the placement plate is matched with the auxiliary part, and the telescopic rod is matched with the compression roller, so that cells in the cell culture bag can be better guided out, and the cell culture device is more practical.

Description

Technical Field

[0001] The utility model relates to the field of cell culture, in particular to a cell harvesting bracket for cell autophagy. Background Art

[0002] Autophagy is an important cellular physiological process, also known as autophagy or intracellular digestion. It is a process in which cells engulf and degrade damaged or obsolete proteins, organelles and other substances in the cell.

[0003] A cell harvesting scaffold is a tool used in experiments such as cell culture or cell engineering. It is usually a scaffold with structural strength to support culture dishes, cell culture bags or other culture containers to better collect and separate cultured cells.

[0004] After searching, Chinese patent announcement number: CN216947053U discloses a cell harvesting support for a cell culture bag, including a base, a support column is provided on the top of the base, a load-bearing plate is provided on the top of the support column, a hook is provided on one side of the top of the load-bearing plate, a fixed plate is provided on the other side, a positioning hole is provided in the middle of the fixed plate, two columns are symmetrically provided between the hook and the fixed plate, a cavity is provided inside the column, a through groove is provided on the surface, a slide bar is provided in the cavity, a slider is connected to the slide bar, a spring is provided on the top of the slider, a pressure plate is provided on one side of the slider, a handle is provided on the top of the pressure plate, a rubber pad is provided on the bottom, an electric push rod is provided on one side of the support column, a control panel is provided on the top of the base, and a counterweight is provided on the bottom. The utility model has a simple structure, high stability, is conducive to pouring out cells, and improves work efficiency.

[0005] Although the above device can assist in pouring out the cells by setting a pressure plate, the pressure plate is set in the middle of the device and its position is relatively fixed, which means that it can only squeeze the middle of the cell culture bag, while the end connected to the culture bag catheter cannot be squeezed well to assist in pouring out the cells, which is not practical. Therefore, a cell harvesting bracket for cell autophagy is proposed to solve the above problem. Utility Model Content

[0006] In order to make up for the above shortcomings, the utility model provides a cell harvesting bracket for cell autophagy, aiming to improve the problem that the pressure plate in the prior art can only squeeze the middle part of the cell culture bag, which is not practical enough.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a cell harvesting bracket for cell autophagy, comprising a base plate, a frame body is fixedly connected to the upper part of the top surface of the base plate, a placement plate is arranged on the upper part of the frame body, a motor is fixedly connected to the right side of the frame body, a hook part is arranged on the rear side of the placement plate, a fixing part is arranged on the front side of the placement plate, an auxiliary part is arranged on the upper part of the placement plate, and vertical plates are fixedly connected to the left and right sides of the top surface of the placement plate.

[0008] As a further description of the above technical solution:

[0009] The left and right parts of the front side of the top surface of the placement plate are both provided with connecting grooves, and the middle part of the rear side of the top surface of the placement plate is provided with a sliding groove.

[0010] As a further description of the above technical solution:

[0011] A sliding opening is provided on the upper part of the vertical plate.

[0012] As a further description of the above technical solution:

[0013] The output end of the motor is fixedly connected with a rotating shaft, the rotating shaft penetrates the frame body and is rotatably connected thereto, and the rotating shaft penetrates the placement plate and is fixedly connected thereto.

[0014] As a further description of the above technical solution:

[0015] The fixing member includes an adjusting rod, which passes through the placement plate and is rotatably connected thereto, a connecting block passes through and is threadedly connected to the outer wall of the adjusting rod, the connecting block is slidably connected to the connecting groove, and a fixing block is fixedly connected to the top of the connecting block.

[0016] As a further description of the above technical solution:

[0017] The hook member comprises a slider, which is slidably connected to a slide groove, a screw rod passes through the middle of the slider and is threadedly connected, the screw rod passes through the placement plate and is rotatably connected thereto, and a hook body is fixedly connected to the top of the slider.

[0018] As a further description of the above technical solution:

[0019] The auxiliary part includes two telescopic rods, a connecting part is provided on the side of the two telescopic rods away from each other, a fixing plate is fixedly connected to the bottom of the telescopic rod, a connecting rod is fixedly connected between the two fixing plates, a pressure roller is provided between the two fixing plates, the connecting rod passes through the pressure roller and is rotatably connected thereto, and a handle passes through and is fixedly connected to the outer wall of the connecting rod.

[0020] As a further description of the above technical solution:

[0021] The connecting member includes a push handle, a stud is fixedly connected to one side of the push handle close to the vertical plate, the outside of the stud passes through and is threadedly connected to a limiting block, a mounting block is fixedly connected to one side of the stud close to the vertical plate, the mounting block is slidably connected to the sliding mouth, a fixing column is fixedly connected to one side of the mounting block close to the telescopic rod, the fixing column is fixedly connected to the telescopic rod, and a baffle is connected to the outside of the fixing column.

[0022] The utility model has the following beneficial effects:

[0023] 1. In the utility model, by arranging the mutual cooperation between the placement plate and the auxiliary parts, and by arranging the cooperation between the telescopic rod and the pressing roller, the device can better export the cells in the cell culture bag, and is more practical.

[0024] 2. In the utility model, by arranging the cooperation between the placement plate, the hook member and the fixing member, the device can fix and connect cell culture bags of different sizes and flow guide tubes of different sizes, which is more practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is an overall three-dimensional schematic diagram of a cell harvesting scaffold for cell autophagy proposed by the utility model;

[0026] Figure 2 This is a disassembled stereoscopic schematic diagram of a cell harvesting scaffold for cell autophagy proposed by the utility model;

[0027] Figure 3 This is a three-dimensional schematic diagram of the bottom plate of a cell harvesting support for cell autophagy proposed by the utility model;

[0028] Figure 4 This is a three-dimensional schematic diagram of a fixing part of a cell harvesting bracket for cell autophagy proposed by the utility model;

[0029] Figure 5 This is a three-dimensional schematic diagram of a hook member of a cell harvesting bracket for cell autophagy proposed by the utility model;

[0030] Figure 6 This is a three-dimensional schematic diagram of an auxiliary component of a cell harvesting bracket for cell autophagy proposed by the utility model.

[0031] Legend:

[0032] 1. Bottom plate; 2. Frame; 3. Placement plate; 4. Hook piece; 5. Fixing piece; 6. Auxiliary piece; 7. Motor; 31. Vertical plate; 32. Connecting groove; 33. Slide groove; 311. Slide; 71. Rotating shaft; 51. Adjusting rod; 52. Connecting block; 53. Fixing block; 41. Screw; 42. Sliding block; 43. Hook body; 61. Telescopic rod; 62. Connecting piece; 63. Fixing plate; 64. Connecting rod; 65. Pressing roller; 66. Carrying handle; 621. Push handle; 622. Stud; 623. Limiting block; 624. Fixing column; 625. Mounting block; 626. Baffle. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] Reference Figure 1 - Figure 2 The utility model provides an embodiment: a cell harvesting bracket for cell autophagy, comprising a bottom plate 1 for supporting the device, a frame body 2 is fixedly connected to the upper part of the top surface of the bottom plate 1, for cooperating with the connection and support of the placement plate 3, a placement plate 3 is arranged on the upper part of the frame body 2, for placing a cell culture bag, a motor 7 is fixedly connected to the right side of the frame body 2, for adjusting the angle of the placement plate 3, a rotating shaft 71 is fixedly connected to the output end of the motor 7, for connecting with the placement plate 3, the rotating shaft 71 passes through the frame body 2 and is rotatably connected thereto, the rotating shaft 71 passes through the placement plate 3 and is fixedly connected thereto, for cooperating with the adjustment of the inclination angle of the placement plate 3, a hook part 4 is arranged on the rear side of the placement plate 3, for fixing the cell culture bag through the fixing port on the cell culture bag, so as to facilitate cell harvesting, a fixing part 5 is arranged on the front side of the placement plate 3, for fixing the guide tube of the cell culture bag, and an auxiliary part 6 is arranged on the upper part of the placement plate 3, for assisting the discharge of cells in the culture bag.

[0035] like Figure 3 As shown, vertical plates 31 are fixedly connected to the left and right sides of the top surface of the placing plate 3 for cooperating with the installation of the auxiliary component 6, connecting grooves 32 are provided on the left and right parts of the front side of the top surface of the placing plate 3 for cooperating with the connection of the fixing component 5, a sliding groove 33 is provided in the middle part of the rear side of the top surface of the placing plate 3 for cooperating with the connection of the hook component 4, and a sliding opening 311 is provided on the upper part of the vertical plate 31 for cooperating with the movement of the auxiliary component 6.

[0036] like Figure 4As shown, the fixing member 5 includes an adjusting rod 51 for adjusting the distance between the two fixing blocks 53, and the left and right parts thereof are provided with threads in opposite directions. The adjusting rod 51 passes through the placement plate 3 and is rotatably connected thereto, and a connecting block 52 passes through and is threadedly connected to the outer wall of the adjusting rod 51 for connecting to the adjusting rod 51, and the connecting block 52 is slidably connected to the connecting groove 32, and a fixing block 53 is fixedly connected to the top of the connecting block 52, and the two fixing blocks 53 are symmetrically arranged, and a fixing groove is provided on one side close to each other for fixing the guide tube of the cell culture bag.

[0037] See also Figure 5 The hook member 4 includes a slider 42 for matching the connection of the hook body 43. The slider 42 is slidably connected to the slide groove 33. A screw 41 passes through the middle of the slider 42 and is threadedly connected to adjust the position of the slider 42 to adapt to the position of cell culture bags of different sizes. The screw 41 passes through the placement plate 3 and is rotatably connected thereto. The top of the slider 42 is fixedly connected to the hook body 43 for fixing the culture bag through the fixing port on the cell culture bag.

[0038] like Figure 6 As shown, the auxiliary component 6 includes two telescopic rods 61, which are composed of a hollow rod and a vertical rod. A spring is fixedly connected between the vertical rod and the hollow rod to cooperate with the extension and retraction. A connecting member 62 is arranged on the side of the two telescopic rods 61 away from each other, which is used to connect with the vertical plate 31. A fixed plate 63 is fixedly connected to the bottom of the telescopic rod 61, which is used to cooperate with the connection of the connecting rod 64. A connecting rod 64 is fixedly connected between the two fixed plates 63, which is used to cooperate with the connection of the pressure roller 65. A pressure roller 65 is arranged between the two fixed plates 63 for squeezing the culture bag to assist the outflow of cells. The connecting rod 64 passes through the pressure roller 65 and is rotatably connected thereto. A handle 66 is passed through and fixedly connected to the outer wall of the connecting rod 64, which is used to lift the pressure roller 65.

[0039] Furthermore, the connecting member 62 includes a push handle 621 for pushing the overall assembly to move. The push handle 621 is fixedly connected to a stud 622 on one side close to the vertical plate 31 for cooperating with the connection of the limit block 623. The outside of the stud 622 passes through and is threadedly connected to the limit block 623 for fixing the position of the connecting member 62 together with the overall auxiliary member 6. The stud 622 is fixedly connected to a mounting block 625 on one side close to the vertical plate 31 for connecting to the slide 311 to limit the angle of the auxiliary member 6. The mounting block 625 is slidably connected to the slide 311. The mounting block 625 is fixedly connected to a fixing column 624 on one side close to the telescopic rod 61 for connecting to the telescopic rod 61. The fixing column 624 is fixedly connected to the telescopic rod 61. The outside of the fixing column 624 is connected to a baffle 626 for limiting the position of the connecting member 62 in the slide 311 to prevent it from moving left and right.

[0040] Working principle: When in use, first, according to the size of the cell culture bag, the screw rod 41 is rotated to drive the slider 42 to slide in the slide groove 33, thereby changing the position of the hook body 43 so that it can adapt to the fixation of cell culture bags of different sizes, and then the fixing mouth at one end of the cell culture bag is put on the hook body 43 to fix it, and then the pressing roller 65 is lifted by the handle 66 to pass the cell culture bag between the pressing roller 65 and the placement plate 3, and then the guide tube at the other end of the cell culture bag is placed between the two fixing blocks 53, and then the adjusting rod 51 is rotated to drive the two connecting blocks 52 to approach each other, so that the two fixing blocks 53 clamp the guide tube to fix the position of the cell culture bag, and then the motor 7 is turned on to drive the rotating shaft 71 to rotate, and the placement plate 3 fixed with the rotating shaft 71 will change its angle accordingly, thereby driving the cell culture bag placed on the upper part thereof to tilt, and assisting the cells to be discharged from the guide tube. When most of the cells are discharged, the remaining cells in the cell culture bag can be The auxiliary part 6 presses the cell culture bag to assist in discharge, and the limit block 623 is rotated to make it away from the vertical plate 31, so that the limit between the auxiliary part 6 and the vertical plate 31 is lost, and then the two push handles 621 are pushed to drive the connecting part 62 to move, and the telescopic rod 61 connected to the connecting part 62 will then drive the connecting rod 64 and the pressure roller 65 connected to the connecting rod 64 to move through the fixed plate 63. Since there is a spring in the telescopic rod 61, it will push the pressure roller 65 to close to the cell culture bag. When the pressure roller 65 moves in the direction of the guide tube, it will assist the residual cells in the culture bag to flow into the guide tube, thereby completing the cell export. When the export is completed, the adjustment rod 51 is rotated in the opposite direction to make the two fixed blocks 53 loosen the guide tube, and then the pressure roller 65 is pulled up again by the handle 66 to extract the cell culture bag from under the pressure roller 65, and the cell culture bag is removed from the hook body 43, and then the auxiliary part 6 is moved to a suitable position, and the limit block 623 is rotated in the opposite direction to limit the auxiliary part 6 and the vertical plate 31.

[0041] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell harvesting support for cell autophagy, comprising a bottom plate (1), characterized in that: The upper part of the top surface of the bottom plate (1) is fixedly connected to a frame body (2), a placement plate (3) is arranged on the upper part of the frame body (2), a motor (7) is fixedly connected to the right side of the frame body (2), a hook member (4) is arranged on the rear side of the placement plate (3), a fixing member (5) is arranged on the front side of the placement plate (3), and an auxiliary member (6) is arranged on the upper part of the placement plate (3); The left and right sides of the top surface of the placement plate (3) are fixedly connected with a vertical plate (31), the left and right parts of the front side of the top surface of the placement plate (3) are provided with a connecting groove (32), the middle part of the rear side of the top surface of the placement plate (3) is provided with a sliding groove (33), the upper part of the vertical plate (31) is provided with a sliding opening (311), the output end of the motor (7) is fixedly connected with a rotating shaft (71), the rotating shaft (71) passes through the frame (2) and is rotatably connected thereto, and the rotating shaft (71) passes through the placement plate (3) and is fixedly connected thereto; The auxiliary component (6) comprises two telescopic rods (61), a connecting component (62) is provided on the side of the two telescopic rods (61) away from each other, a fixing plate (63) is fixedly connected to the bottom of the telescopic rod (61), a connecting rod (64) is fixedly connected between the two fixing plates (63), a pressure roller (65) is provided between the two fixing plates (63), the connecting rod (64) passes through the pressure roller (65) and is rotatably connected thereto, a handle (66) passes through and is fixedly connected to the outer wall of the connecting rod (64), and the connecting component (62) comprises a push handle (621). The push handle (621) is fixedly connected to a stud (622) on one side close to the vertical plate (31); the outside of the stud (622) passes through and is threadedly connected to a limit block (623); the stud (622) is fixedly connected to a mounting block (625) on one side close to the vertical plate (31); the mounting block (625) is slidably connected to the sliding opening (311); the mounting block (625) is fixedly connected to a fixing column (624) on one side close to the telescopic rod (61); the fixing column (624) is fixedly connected to the telescopic rod (61); the outside of the fixing column (624) is connected to a baffle (626).

2. The cell harvesting scaffold for cell autophagy according to claim 1, characterized in that: The fixing member (5) comprises an adjusting rod (51), the adjusting rod (51) passes through the placement plate (3) and is rotatably connected thereto, a connecting block (52) passes through and is threadedly connected to the outer wall of the adjusting rod (51), the connecting block (52) is slidably connected to the connecting groove (32), and a fixing block (53) is fixedly connected to the top of the connecting block (52).

3. The cell harvesting scaffold for cell autophagy according to claim 1, characterized in that: The hook member (4) comprises a slider (42), the slider (42) is slidably connected to the slide groove (33), a screw rod (41) passes through the middle of the slider (42) and is threadedly connected, the screw rod (41) passes through the placement plate (3) and is rotatably connected thereto, and a hook body (43) is fixedly connected to the top of the slider (42).

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