Sterile spray-washing device for cell culture spinner bottle

Abstract

The utility model relates to the technical field of laboratory equipment, in particular to a cell culture spinner bottle sterile spray washing device which comprises a spraying mechanism used for washing a spinner bottle, a containing mechanism used for containing waste liquid and a bottle rack used for supporting the spinner bottle. The bottle rack comprises a bearing plate supported above an opening of the containing barrel, and the spraying mechanism comprises a spraying pipe which is fixed to the bearing plate in an upward penetrating mode. The serum removal device has the effect of efficiently completing the serum removal work in the culture solution.

Description

Technical Field

[0001] The present application relates to the technical field of laboratory equipment, and in particular to a sterile spray washing device for cell culture rotating bottles. Background Art

[0002] In the biopharmaceutical industry, spinner flask culture of adherent cells is often used, such as the culture of Vero cells. The culture medium for culturing cells often contains serum, which can provide nutrients, promote cell growth, and maintain cell stability.

[0003] Serum plays a huge role in cell production, but for finished vaccines, the serum content needs to be reduced as much as possible. The pharmacopoeia requires that the serum albumin content of finished vaccines be less than 50 ng / dose. If the serum removal process relies entirely on downstream concentration and purification, the downstream process will face tremendous pressure and difficulties. Therefore, a controllable, sterile, and fast device is needed to assist in the removal of serum from the culture medium without affecting cell growth. Utility Model Content

[0004] In order to efficiently complete the removal of serum from the culture medium, the present application provides a sterile spray washing device for a cell culture rotating bottle.

[0005] The present application provides a cell culture rotating bottle sterile spray washing device adopts the following technical solution:

[0006] A sterile spray washing device for a cell culture rotating bottle comprises a spray mechanism for washing the rotating bottle, a receiving mechanism for receiving waste liquid, and a bottle rack for supporting the rotating bottle. The receiving mechanism comprises a receiving barrel with an open top, the bottle rack comprises a supporting plate supported above the opening of the receiving barrel, and the spray mechanism comprises a spray pipe which is upwardly penetrated and fixed on the supporting plate.

[0007] By adopting the above technical solution, the experimenter first sterilizes these structures at high temperature, then pours the liquid in the rotating bottle into the receiving bucket, places the rotating bottle on the support plate, and places the spray pipe in the rotating bottle. Then, spray liquid is passed into the spray pipe to clean the rotating bottle. The waste liquid after cleaning can flow into the receiving bucket. During the cleaning process, the experimenter can also rotate the rotating bottle to achieve a better cleaning effect. By using the above device, the removal of serum in the culture medium can be completed efficiently.

[0008] Optionally, the bottle rack further comprises a fixing plate located below the supporting plate, a plurality of mounting rods are fixedly connected between the fixing plate and the supporting plate, a plurality of fixing rods are fixedly connected between the fixing plate and the receiving barrel, and the spray pipe is passed through and fixed on the fixing plate.

[0009] By adopting the above technical solution, the supporting plate is supported by the structure composed of the fixed plate, the erecting rods and the fixed rods, and can maintain a stable state. The supporting structure is stable.

[0010] Optionally, a reinforcing plate is provided below the fixing plate, a reinforcing rod is fixedly connected between the reinforcing plate and the fixing plate, and the spray pipe is passed through and fixed on the reinforcing plate.

[0011] By adopting the above technical solution, the reinforcement plate and the reinforcement rod cooperate with each other to improve the stability of the sprinkler pipe.

[0012] Optionally, the supporting plate is umbrella-shaped.

[0013] By adopting the above technical solution, it is convenient to place the spray pipe on the central axis of the rotating bottle, so as to improve the cleaning effect of all directions of the bottles in the rotating bottle.

[0014] Optionally, a plurality of ridges are evenly arranged on the top surface of the support plate, and the ridges extend from the center of the support plate to the edge thereof.

[0015] By adopting the above technical solution, the convex ridges have a supporting effect on the rotating bottle, so that a certain gap can be formed between the rotating bottle and the supporting plate, which is convenient for the waste liquid to flow out.

[0016] Optionally, the spray mechanism further includes a pump body and a liquid storage barrel, a ventilation pipe is connected between the pump body and the liquid storage barrel, and a liquid outlet pipe is connected between the liquid storage barrel and the spray pipe.

[0017] By adopting the above technical solution, the spray liquid is introduced into the liquid storage barrel, and then the pump body is turned on to pump the spray liquid into the spray pipe. Different spray liquids can be introduced into the liquid storage barrel as needed.

[0018] Optionally, a sterile joint is installed between the liquid outlet pipe and the spray pipe.

[0019] By adopting the above technical solution, the spray mechanism and other structures can be separated for sterilization operation.

[0020] Optionally, it further includes a waste liquid recovery mechanism, which includes a recovery barrel, and a waste liquid pipe is connected between the receiving barrel and the recovery barrel.

[0021] By adopting the above technical solution, the waste liquid in the receiving barrel can be introduced into the recovery barrel through the waste liquid pipe, so as to facilitate the timely dumping of the waste liquid.

[0022] In summary, the present application includes at least one of the following beneficial technical effects:

[0023] 1. The experimenter first sterilizes these structures at high temperature, then pours the liquid in the rotating bottle into the receiving bucket, places the rotating bottle on the supporting plate, places the spray pipe in the rotating bottle, and then passes the spray liquid into the spray pipe to clean the rotating bottle. The waste liquid after cleaning can flow into the receiving bucket. During the cleaning process, the experimenter can also rotate the rotating bottle to achieve a better cleaning effect. By using the above device, the serum in the culture medium can be removed efficiently;

[0024] 2. The support plate is supported by a structure consisting of a fixed plate, a stand rod and a fixed rod, and can maintain a stable state. The support structure is stable;

[0025] 3. Introduce the spray liquid into the liquid storage barrel, then turn on the pump body to pump the spray liquid into the spray pipe. Different spray liquids can be introduced into the liquid storage barrel as needed. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of a cell culture rotating bottle sterile spray washing device in Example 1 of the present application.

[0027] Figure 2 yes Figure 1 Cross-sectional view of the middle receiving barrel.

[0028] Figure 3 It is a schematic diagram of the structure of the support plate and the ridges in Example 2 of the present application.

[0029] Explanation of the reference numerals: 1. spray mechanism; 10. pump body; 11. liquid storage barrel; 12. ventilation pipe; 13. liquid outlet pipe; 14. spray pipe; 15. sterile connector; 2. receiving mechanism; 20. bracket; 21. receiving barrel; 3. bottle rack; 30. fixing plate; 31. fixing rod; 32. stand rod; 33. supporting plate; 330. convex ridge; 34. reinforcing plate; 35. reinforcing rod; 4. waste liquid recovery mechanism; 40. recovery barrel; 41. waste liquid pipe. DETAILED DESCRIPTION

[0030] The following is combined with Figure 1 -Attached Figure 3 This application is described in further detail.

[0031] The embodiment of the present application discloses a sterile spray washing device for cell culture rotating bottles.

[0032] Example 1

[0033] Reference Figure 1 A cell culture rotating bottle aseptic spray washing device comprises a spray mechanism 1 for spraying a spray washing liquid, a receiving mechanism 2 for temporarily receiving waste liquid, a bottle rack 3 for supporting the rotating bottle, and a waste liquid recovery mechanism 4 for recovering waste liquid.

[0034] Reference Figure 1 The spray mechanism 1 includes a pump body 10 and a liquid storage barrel 11. The pump body 10 in this embodiment is a positive and negative pressure pump. The liquid storage barrel 11 can contain different spraying liquids according to process requirements. A vent pipe 12 is connected between the pump body 10 and the liquid storage barrel 11. A liquid outlet pipe 13 is also connected to the liquid storage barrel 11. A spray pipe 14 is connected to the end of the liquid outlet pipe 13 away from the liquid storage barrel 11. In order to facilitate the separate treatment of the liquid outlet pipe 13 and the spray pipe 14, a sterile joint 15 is installed between the liquid outlet pipe 13 and the spray pipe 14.

[0035] Reference Figure 1 The receiving mechanism 2 includes a bracket 20 supported on the ground. The bracket 20 is in the shape of a rectangular frame. A receiving barrel 21 is fixedly installed in the bracket 20. The receiving barrel 21 is suspended. The top of the receiving barrel 21 is open. The waste liquid after washing the rotating bottle enters the receiving barrel 21 from top to bottom.

[0036] Reference Figure 1 and Figure 2 The bottle rack 3 includes a fixing plate 30, which is a horizontally arranged circular plate. The fixing plate 30 is located in the receiving barrel 21. A plurality of fixing rods 31 are evenly fixed between the edge of the fixing plate 30 and the top edge of the receiving barrel 21, so that the fixing plate 30 is firmly fixed. A plurality of racking rods 32 are evenly fixed vertically on the edge of the top surface of the fixing plate 30. A supporting plate 33 is fixed on the top of the plurality of racking rods 32. The supporting plate 33 is a circular plate and is parallel to the fixing plate 30. The supporting plate 33 is used to support the rotating bottles.

[0037] Reference Figure 2 A reinforcing plate 34 is arranged parallel to and below the fixing plate 30. The reinforcing plate 34 is a circular plate, and its area is smaller than that of the fixing plate 30. A plurality of reinforcing rods 35 are evenly fixed between the edge of the reinforcing plate 34 and the edge of the fixing plate 30. The spray pipe 14 is sequentially passed through and fixed on the reinforcing plate 34, the fixing plate 30 and the supporting plate 33, so that the spray pipe 14 is stably fixed.

[0038] Reference Figure 1 The waste liquid recovery mechanism 4 includes a recovery barrel 40. A waste liquid pipe 41 is connected between the recovery barrel 40 and the receiving barrel 21. The waste liquid in the receiving barrel 21 can enter the recovery barrel 40 through the waste liquid pipe 41. A valve can be installed on the waste liquid pipe 41. When the waste liquid in the recovery barrel 40 accumulates to a certain level, the valve can be closed to treat the waste liquid in the recovery barrel 40.

[0039] The implementation principle of Example 1 of the present application is as follows: before operation, disconnect the sterile connector 15, sterilize the spray pipe 14, the bottle rack 3, the receiving bucket 21 and the waste liquid pipe 41 as a whole under high pressure for standby use, and sterilize the recovery bucket 40 separately for standby use. After sterilization, move the above structure to the fan for air drying, and then install the sterile connector 15, and use different spraying liquids according to process requirements. Pour the liquid in the rotating bottle to be cleaned into the receiving bucket 21, and then invert the rotating bottle on the support plate 33 so that the spray pipe 14 is located in the rotating bottle. Connect the vent pipe 12 of the pump body 10 to the liquid storage barrel 11, turn on the positive pressure, and the spraying liquid is sprayed to the bottom of the rotating bottle through the spray pipe 14, and then the entire rotating bottle is flushed along the inner wall of the rotating bottle. During the spraying process, the rotating bottle can be manually rotated to make the spraying more thorough. The spraying device is easy to operate and can efficiently complete the removal of serum from the culture medium.

[0040] Example 2

[0041] Reference Figure 3 The difference between this embodiment and embodiment 1 is that the support plate 33 in this embodiment is umbrella-shaped, and a plurality of ridges 330 are fixedly connected to the top surface of the support plate 33 from the center of the support plate 33 to its edge. The plurality of ridges 330 are evenly arranged along the center of the support plate 33, and the cross-section of the ridges 330 is triangular.

[0042] The implementation principle of Example 2 of the present application is: the rotating bottle is placed on the supporting plate 33. Due to the unique design of the supporting plate 33, the spray pipe 14 can be located on the central axis of the rotating bottle, so that the inner wall in all directions can be evenly sprayed; at the same time, the ridge 330 supports the rotating bottle, so that a gap is left between the edge of the rotating bottle opening and the supporting plate 33 for the waste liquid to flow out, which can facilitate more thorough and faster discharge of the waste liquid.

[0043] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A sterile spray washing device for cell culture rotating bottles, characterized in that: The invention comprises a spray mechanism (1) for washing rotating bottles, a receiving mechanism (2) for receiving waste liquid, and a bottle rack (3) for supporting rotating bottles, wherein the receiving mechanism (2) comprises a receiving barrel (21) with an open top, the bottle rack (3) comprises a supporting plate (33) supported above the opening of the receiving barrel (21), and the spray mechanism (1) comprises a spray pipe (14) which is upwardly penetrated and fixed on the supporting plate (33).

2. A cell culture roller bottle sterile spray washing device according to claim 1, characterized in that: The bottle rack (3) further comprises a fixing plate (30) located below the supporting plate (33); a plurality of mounting rods (32) are fixedly connected between the fixing plate (30) and the supporting plate (33); a plurality of fixing rods (31) are fixedly connected between the fixing plate (30) and the receiving barrel (21); and the spray pipe (14) is passed through and fixed on the fixing plate (30).

3. A cell culture rotating bottle sterile spray washing device according to claim 2, characterized in that: A reinforcing plate (34) is provided below the fixing plate (30), a reinforcing rod (35) is fixedly connected between the reinforcing plate (34) and the fixing plate (30), and the spray pipe (14) is passed through and fixed on the reinforcing plate (34).

4. The aseptic spray washing device for cell culture rotating bottles according to claim 1, characterized in that: The supporting plate (33) is in an umbrella shape.

5. The aseptic spray washing device for cell culture rotating bottles according to claim 4, characterized in that: A plurality of ridges (330) are evenly arranged on the top surface of the supporting plate (33), and the ridges (330) extend from the center of the supporting plate (33) to the edge thereof.

6. A cell culture roller bottle sterile spray washing device according to any one of claims 1 to 5, characterized in that: The spray mechanism (1) further comprises a pump body (10) and a liquid storage barrel (11); a vent pipe (12) is connected between the pump body (10) and the liquid storage barrel (11); and a liquid outlet pipe (13) is connected between the liquid storage barrel (11) and the spray pipe (14).

7. A cell culture rotating bottle sterile spray washing device according to claim 6, characterized in that: A sterile joint (15) is installed between the liquid outlet pipe (13) and the spray pipe (14).

8. A cell culture roller bottle sterile spray washing device according to any one of claims 1 to 5, characterized in that: It also comprises a waste liquid recovery mechanism (4), the waste liquid recovery mechanism (4) comprises a recovery barrel (40), and a waste liquid pipe (41) is connected between the receiving barrel (21) and the recovery barrel (40).

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