Pretreatment device for surface enhanced Raman spectroscopy

Abstract

The utility model discloses a pretreatment device for surface enhanced Raman spectroscopy, which relates to the technical field of Raman spectroscopy, and is characterized in that the pretreatment device comprises a base, a reagent box, a control screen and a miniature oscillator are arranged on the base, a reaction container is arranged on the miniature oscillator, and the reaction container is provided with a reaction cavity. A first liquid inlet, a second liquid inlet and a sample opening are formed in the reaction container, a first reagent tube and a second reagent tube are further arranged on the base, the first reagent tube is connected with the first liquid inlet through a first hose, the second reagent tube is connected with the second liquid inlet through a second hose, and liquid pumps are arranged on the first hose and the second hose. And an upper cover for sealing the sample opening is arranged on the reaction container. According to the pretreatment device, the first reagent tube and the second reagent tube are arranged, so that nano-silver is better adsorbed on the surfaces of bacteria, and the problem that the nano-silver in a silver colloid solution in an existing pretreatment device is not easily adsorbed on the surfaces of the bacteria is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of Raman spectroscopy, and more specifically, to a preprocessing device for surface enhanced Raman spectroscopy technology. Background Art

[0002] A preprocessing device for surface enhanced Raman spectroscopy is used to attach nano silver particles to the surface of an object to be tested, thereby enhancing its Raman scattering signal.

[0003] In the prior art, the pretreatment device used for surface enhanced Raman spectroscopy technology adds nanosilver colloidal solution to the bacterial sample and uses a micro-oscillator to adsorb the nanosilver on the bacterial surface. The direct adsorption of nanosilver on the bacterial surface requires a lot of oscillation time, the reaction is slow and the adsorption is uneven.

[0004] Therefore, new solutions need to be proposed to solve this problem. Utility Model Content

[0005] In view of the shortcomings of the prior art, the purpose of the utility model is to provide a pretreatment device for surface enhanced Raman spectroscopy technology, which can evenly disperse silver particles in a solvent.

[0006] The above technical purpose of the utility model is achieved through the following technical scheme: a pretreatment device for surface enhanced Raman spectroscopy technology, including a base, a reagent box, a control screen and a micro-oscillator are arranged on the base, a reaction container is arranged on the micro-oscillator, and a liquid inlet 1, a liquid inlet 2 and a sample port are opened on the reaction container. A reagent tube 1 and a reagent tube 2 are also arranged on the base, the reagent tube 1 is connected to the liquid inlet 1 through a hose 1, and the reagent tube 2 is connected to the liquid inlet 2 through a hose 2. Liquid pumps are arranged on the hose 1 and the hose 2, and an upper cover for closing the sample port is arranged on the reaction container.

[0007] The utility model is further configured as follows: the control screen includes a control board, the control screen includes a control board, the two liquid pumps are electrically connected to the control board, and the micro-oscillator is electrically connected to the control board.

[0008] The utility model is further configured as follows: the liquid pump is a peristaltic pump.

[0009] The utility model is further configured as follows: both ends of the hose 1 and the hose 2 are provided with connecting parts, the connecting parts include an inner layer and an outer layer, and a clamping groove is provided between the inner layer and the outer layer.

[0010] The utility model is further configured as follows: the first hose and the second hose are both made of polytetrafluoroethylene material.

[0011] In summary, the utility model has the following beneficial effects:

[0012] The utility model is provided with a reagent tube 1 and a reagent tube 2, wherein the reagent tube 1 is connected with a liquid inlet 1 through a hose 1, and the reagent tube 2 is connected with a liquid inlet 2 through a hose 2, and a liquid pump is provided on the hose 1 and the hose 2. When in use, a bacterial sample is first added through the sample port, a micro-oscillator is turned on, and then the liquid pump is controlled to pump the silver nitrate solution in the reagent tube 1 into a reaction container, and since the bacterial cell wall has electronegativity, positively charged silver ions will be adsorbed on the bacterial surface, and then the liquid pump is controlled to pump the reducing agent in the reagent tube 2 into the reaction container, and the silver ions adsorbed on the bacterial surface will be reduced to form nano silver particle clusters, so that the nano silver is better adsorbed on the bacterial surface, and the problem that the nano silver in the silver colloid solution in the existing pretreatment device is not easy to be adsorbed on the bacterial surface is avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of the utility model;

[0014] Figure 2 It is a structural schematic diagram of the connecting pipe in the utility model.

[0015] In the figure: 1. base; 2. micro-oscillator; 3. reaction container; 4. liquid inlet 1; 5. liquid inlet 2; 6. sample port; 7. reagent tube 1; 8. reagent tube 2; 9. upper cover; 10. inner layer; 11. outer layer; 12. snap-in slot. DETAILED DESCRIPTION

[0016] The utility model is described in detail below in conjunction with the accompanying drawings and embodiments.

[0017] Embodiment: A pretreatment device for surface enhanced Raman spectroscopy technology, such as Figure 1 , Figure 2As shown, it includes a base 1, on which a reagent box, a control screen and a micro-oscillator 2 are arranged, on which a reaction container 3 is arranged, on which a liquid inlet 1 4, a liquid inlet 2 5 and a sample port 6 are provided, on which a reagent tube 1 7 and a reagent tube 2 8 are also arranged, the reagent tube 1 7 is connected to the liquid inlet 1 4 through a hose 1, the reagent tube 2 8 is connected to the liquid inlet 2 5 through a hose 2, and the hose 1 and the hose 2 are both provided with a liquid pump (not shown in the figure), and the reaction container 3 is provided with an upper cover 9 for closing the sample port 6, the reagent tube 1 7 is used to store a silver nitrate solution, and the reagent tube 2 8 is used to store The reducing agent is more specifically hydroxylamine hydrochloride. When in use, first add the bacterial sample through the sample port 6, turn on the micro-oscillator 2, and then control the liquid pump to pump the silver nitrate solution in the reagent tube 1 7 into the reaction container 3. Due to the electronegativity of the bacterial cell wall, the positively charged silver ions will be adsorbed on the bacterial surface. Then control the liquid pump to pump the reducing agent in the reagent tube 2 8 into the reaction container 3. The silver ions adsorbed on the bacterial surface will be reduced to form nanosilver particle clusters, so that the nanosilver is better adsorbed on the bacterial surface, avoiding the problem that the nanosilver in the silver colloid solution in the existing pretreatment device is not easy to adsorb on the bacterial surface.

[0018] The control panel includes a control board, the liquid pump is electrically connected to the control board, and the micro-oscillator 2 is electrically connected to the control board. More specifically, the control board is a single-chip microcomputer that can automatically control the switch and power of the liquid pump and the micro-oscillator 2, thereby improving the processing accuracy.

[0019] The liquid pump is a peristaltic pump, which has high precision and can accurately control the amount of pumped liquid. The peristaltic pump does not directly contact the reagent and can prevent contamination.

[0020] Both ends of hose one and hose two are provided with connecting parts, and the connecting parts include an inner layer 10 and an outer layer 11. A clamping groove 12 is provided between the inner layer 10 and the outer layer 11. Liquid inlet one 4 and liquid inlet two 5 can be clamped into the clamping groove 12, which can increase the contact area between liquid inlet one 4 and liquid inlet two 5 and the hose, so that the hose connection has better sealing performance.

[0021] Hose 1 and hose 2 are both made of polytetrafluoroethylene material. Polytetrafluoroethylene material has good corrosion resistance and is not easy to react with reagents, so that hose 1 and hose 2 have better chemical resistance.

[0022] The above is only a preferred embodiment of the present invention. The protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A pretreatment device for surface enhanced Raman spectroscopy technology, comprising a base (1), on which a reagent box, a control screen and a micro-oscillator (2) are arranged, characterized in that: The micro-oscillator (2) is provided with a reaction container (3), and the reaction container (3) is provided with a liquid inlet port 1 (4), a liquid inlet port 2 (5) and a sample port (6). The base (1) is also provided with a reagent tube 1 (7) and a reagent tube 2 (8). The reagent tube 1 (7) is connected to the liquid inlet port 1 (4) through a hose 1, and the reagent tube 2 (8) is connected to the liquid inlet port 2 (5) through a hose 2. Liquid pumps are provided on the hose 1 and the hose 2. The reaction container (3) is provided with an upper cover (9) for sealing the sample port (6).

2. A pretreatment device for surface enhanced Raman spectroscopy according to claim 1, characterized in that: The control screen comprises a control panel, the two liquid pumps are electrically connected to the control panel, and the micro-oscillator (2) is electrically connected to the control panel.

3. The pretreatment device for surface enhanced Raman spectroscopy according to claim 1, characterized in that: The liquid pump is a peristaltic pump.

4. The pretreatment device for surface enhanced Raman spectroscopy according to claim 1, characterized in that: Both ends of the first hose and the second hose are provided with connecting parts, the connecting parts include an inner layer (10) and an outer layer (11), and a clamping groove (12) is provided between the inner layer (10) and the outer layer (11).

5. The pretreatment device for surface enhanced Raman spectroscopy according to claim 1, characterized in that: The hose 1 and the hose 2 are both made of polytetrafluoroethylene material.

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