Microporous filter plate negative pressure device

Abstract

The utility model provides a microporous filter plate negative pressure device which comprises a base, a collecting plate, a support, a filter plate and a sealing cover, a through hole is formed in the support in the vertical direction in a penetrating mode, a supporting part is arranged on the periphery of the through hole of the support, the support is arranged in a mounting groove, a plurality of sample adding holes are formed in the filter plate, and a positioning part is arranged on the periphery of the sample adding holes of the filter plate. The filter plate is mounted in the through hole, the supporting part is connected with the positioning part, and one sample outlet is communicated with one collecting opening; a hole sealing part is arranged in the middle of the bottom surface of the sealing cover, an edge sealing part is arranged on the periphery of the hole sealing part on the bottom surface of the sealing cover, the sealing cover covers the mounting port, the hole sealing part hermetically covers the plurality of sample adding ports, and the edge sealing part hermetically covers the upper end surface of the bracket and the edge of the mounting port. According to the scheme, the number of accessories is small, assembling is easy and convenient, automatic adsorption is achieved in the suction process by means of the overall sealing installation opening of the sealing cover, suction is tighter in the suction process, and therefore manual pressing is not needed any more.

Description

Technical Field

[0001] The utility model relates to the technical field of filtration, in particular to a microporous filter plate negative pressure device. Background Art

[0002] The microporous filter plate negative pressure device is specially designed for applications such as protein kinase, phosphatase analysis, protein purification, receptor action analysis, protein binding assay, ELISPOT analysis, sample preparation, mass spectrometry analysis sample filtration processing, and fluorescent dye removal. It can be adapted to 96-well microporous filter plates, avoiding repeated aspiration, centrifugation, and pipetting operations in traditional methods, thereby simplifying the experimental steps.

[0003] The existing microporous filter plate negative pressure device is sealed mainly by manual pressure holding, that is, by manually pressing the filter plate, a relatively closed cavity is formed between the filter plate and the mounting groove of the seat body, and then the vacuum generator is used to evacuate the closed cavity, and then under the action of negative pressure, the sample on the filter plate drips into the collection plate through the micropores. Since it needs to be pressed by hand during operation, it affects the convenience and convenience of operation. Utility Model Content

[0004] The utility model aims to provide a microporous filter plate negative pressure device which is easy to assemble and use.

[0005] In order to achieve the purpose of the utility model, the utility model provides a microporous filter plate negative pressure device, including a base and a collecting plate, the base is provided with a mounting groove, the mounting groove is provided with a bottom wall and an inner peripheral wall located at the outer periphery of the bottom wall, the mounting groove has a mounting opening vertically above the bottom wall, the inner peripheral wall is penetrated with a negative pressure exhaust hole, the collecting plate is provided with a plurality of collecting holes, the collecting holes have a collecting opening vertically above, and the collecting plate is arranged in the mounting groove; the microporous filter plate negative pressure device also includes a bracket, a filter plate and a sealing cover, the bracket is penetrated with a through hole in the vertical direction, the bracket is provided with a support portion at the outer periphery of the through hole, and the bracket is provided with In the installation groove, the outer wall of the bracket is connected to the inner wall; the filter plate is provided with a plurality of sample addition holes, the sample addition holes have a sample addition port vertically above, and the sample addition holes have a sample outlet vertically below, the filter plate is provided with a positioning part on the periphery of the plurality of sample addition holes, the filter plate is installed in the through hole, the support part is connected to the positioning part, and a sample outlet is communicated with a collection port; a hole sealing part is provided in the middle of the bottom surface of the sealing cover, and an edge sealing part is provided on the bottom surface of the sealing cover on the periphery of the hole sealing part, the sealing cover is closed at the installation port, the hole sealing part tightly covers the plurality of sample addition ports, and the edge sealing part tightly covers the upper end surface of the bracket and the edge of the installation port.

[0006] A further solution is that the hole sealing portion includes a plurality of sealing bosses, sealing grooves are provided between the plurality of sealing bosses, a sealing boss is inserted into a sample adding port, and the edge of the sample adding port is located in the sealing groove.

[0007] A further solution is that the edge sealing portion includes an inner ring, an outer ring, an annular groove and an outer edge, the outer ring is located on the outer periphery of the inner ring, the outer ring, the inner ring and the bottom surface of the sealing cover form an annular groove, the outer edge is located on the outer periphery of the outer ring, the upper end surface of the bracket is located in the annular groove and adjacent to the bottom surface of the sealing cover, the inner ring and the outer ring are adjacent to the bracket respectively, and the outer edge is adjacent to the edge of the mounting port.

[0008] A further solution is that the inner peripheral wall is provided with a first supporting step, and the bracket is arranged on the first supporting step.

[0009] A further solution is that the supporting portion is arranged in the form of a second supporting step, the positioning portion is arranged in the form of a ring platform, and the ring platform is arranged on the second supporting step.

[0010] A further solution is that the plurality of sample addition holes all pass through the through hole.

[0011] A further solution is that a positioning ring is provided on the periphery of the sample outlet, and the positioning ring is located in the collecting hole and is in clearance fit with the collecting hole.

[0012] A further solution is that a handle protrusion is provided on the upper end surface of the bracket, and a groove is provided through the sealing cover, and the handle protrusion passes through the groove.

[0013] A further solution is that two handle protrusions are provided on the upper end surface of the bracket, and the two handle protrusions are respectively located on two horizontally opposite sides of the through hole, and two through grooves are provided through the sealing cover, and one handle protrusion passes through one through groove.

[0014] A further solution is that the sealing cover is made of silicone material.

[0015] The beneficial effect of the utility model is that the bracket is installed and positioned in the installation groove, and then the filter plate is set on the bracket, and then a sample addition hole can be arranged corresponding to a collection hole, and then the sealing cover is closed. Since the sealing cover covers the sample addition port, the upper end surface of the bracket and the edge of the installation groove, the installation groove is relatively closed by utilizing the overall sealing installation port, and then the negative pressure suction hole is used for suction. Through the negative pressure of the installation groove, the sample on the filter plate drips into the collection plate through the micropores. This case not only has few accessories and is easy to assemble, but also utilizes the overall sealing installation port of the sealing cover, which automatically adsorbs during the suction process, and the more suction is done, the tighter it is. Furthermore, the use of silicone material can further improve the sealing effect, so manual pressing is no longer required. In addition, through the specific structure of the edge sealing part and the hole sealing part, it can further improve the sealing effect, and through the arrangement of the support step, the assembly and positioning of the bracket and the filter plate are made simpler. And the setting of the handle protrusion facilitates the removal of the collection plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural diagram of an embodiment of a microporous filter plate negative pressure device of the utility model.

[0017] Figure 2 This is the first exploded view of an embodiment of the microporous filter plate negative pressure device of the utility model.

[0018] Figure 3 This is the second exploded view of the microporous filter plate negative pressure device embodiment of the utility model.

[0019] Figure 4 It is a cross-sectional view of an embodiment of a microporous filter plate negative pressure device of the utility model.

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments. DETAILED DESCRIPTION

[0021] Reference Figures 1 to 4 The microporous filter plate negative pressure device includes a base 1, a collecting plate 21, a bracket 23, a filter plate 22 and a sealing cover 24. The base 1 is arranged in a rectangular shape. The base 1 is provided with a mounting groove 11. The mounting groove 11 is provided with a bottom wall and an inner circumferential wall located on the outer periphery of the bottom wall. The mounting groove 11 has a mounting port 12 vertically above the bottom wall. A negative pressure exhaust hole 12 is penetrated through the inner circumferential wall. The negative pressure exhaust hole 12 is used to connect a vacuum generator to realize exhaust of the mounting groove 11. The inner circumferential wall is also provided with a first supporting step 14.

[0022] The collecting plate 21 is provided with a plurality of collecting holes 211, which are arranged as blind holes, and have a collecting port vertically above the collecting holes 211, and the collecting plate 21 is arranged in the mounting groove 11. The bracket 23 is arranged as a rectangular frame, and a through hole 231 is provided through the bracket 23 in the vertical direction, and a supporting portion 232 is provided on the outer periphery of the through hole 231 of the bracket 23, and the supporting portion 232 is provided as a second supporting step.

[0023] The bracket 23 is disposed in the mounting groove 11 , and the outer wall of the bracket 23 is connected to the inner peripheral wall. Specifically, the bracket 23 is disposed on and supported by the first supporting step 14 , and a matching gap exists between the bracket 23 and the first supporting step 14 .

[0024] The filter plate 22 is provided with a plurality of sample loading holes 221, the sample loading holes 221 have a sample loading port 225 vertically above, and the sample loading holes 221 have a sample outlet 222 vertically below, the sample outlet 222 extends downward through a guide column, and a positioning ring 223 is provided on the periphery of the sample outlet 222. The filter plate 22 is provided with a positioning portion 224 on the periphery of the plurality of sample loading holes 221, and the positioning portion 224 is arranged in a ring stage, the filter plate 22 is installed in the through hole 231, and the support portion 232 is connected to the positioning portion 224, that is, the ring stage is arranged on the second support step and supported by the second support step.

[0025] At this time, multiple sample addition holes 221 all pass through the through hole 231, and one sample addition hole 221 can be arranged corresponding to one collection hole 211. The positioning ring 223 is located in the collection hole 211 and has a gap fit with the collection hole 211. One sample outlet 222 is connected to one collection port, and the outer wall of the sample addition hole 221 has a gap fit with the outer wall of the collection hole 211, rather than a closed fit.

[0026] The sealing cover 24 is made of silicone material, and a hole sealing portion 241 is arranged in the middle of the bottom surface of the sealing cover 24, and the hole sealing portion 241 includes a plurality of sealing bosses 242 extending downward, and a sealing groove is arranged between the plurality of sealing bosses 242, and an edge sealing portion 243 is arranged on the bottom surface of the sealing cover 24 at the periphery of the hole sealing portion 241, and the edge sealing portion 243 includes an inner ring 245, an outer ring 246, an annular groove 244 and an outer edge 247, and the outer ring 246 is located at the periphery of the inner ring 245, and the outer ring 246, the inner ring 245 and the bottom surface of the sealing cover 24 form an annular groove 244, and the cross-section of the annular groove 244 is U-shaped, and the outer edge 247 is located at the periphery of the outer ring 246.

[0027] The sealing cover 24 covers the installation port 12, the hole sealing portion 241 seals and covers multiple sample injection ports 225, and the edge sealing portion 243 seals and covers the upper end surface 233 of the bracket 23 and the edge of the installation port 12. Specifically, a sealing boss 242 is inserted into a sample injection port 225, and the edge of the sample injection port 225 is located in the sealing groove, thereby forming a seal for the sample injection hole 221, and the upper end surface of the bracket 23 is located in the annular groove 244 and adjacent to the bottom surface of the sealing cover 24, the inner ring 245 and the outer ring 246 are adjacent to the bracket 23 respectively, and the outer ring 246 is inserted into the matching gap, and the outer edge 247 is adjacent to the edge of the installation port 12, thereby realizing the overall sealing of the base 1, the bracket 23 and the installation port 12.

[0028] Two handle protrusions 234 are provided on the upper end surface of the bracket 23, and the two handle protrusions 234 are respectively located on the horizontal opposite sides of the through hole 231. The sealing cover 24 is penetrated by two through grooves 248, and one handle protrusion 234 passes through one through groove 248. The edge of the through groove 248 is adjacent to the upper end surface 233 around the handle protrusion 234, thereby not affecting the overall sealing of the installation port 12.

[0029] When performing microporous filtration, first install the collection plate 21 into the base 1, and add sample liquid on the filter plate 22, then set the bracket 23 in the installation groove 11, and then cover the sealing cover 24 on the installation port 12, and finally turn on the vacuum generator to evacuate the installation groove 11. Through the negative pressure of the installation groove, the sample on the filter plate is filtered through micropores and drips into the collection plate. This case not only has few accessories and is easy to assemble, but also utilizes the overall sealing installation port of the sealing cover, which automatically adsorbs during the suction process, and the more suction is done, the tighter it is. Furthermore, silicone material is used, which can further improve the sealing effect, so manual pressing is no longer required.

Claims

1. A microporous filter plate negative pressure device, comprising a base and a collecting plate, wherein the base is provided with a mounting groove, the mounting groove is provided with a bottom wall and an inner peripheral wall located at the outer periphery of the bottom wall, the mounting groove has a mounting opening vertically above the bottom wall, the inner peripheral wall is penetrated with a negative pressure suction hole, the collecting plate is provided with a plurality of collecting holes, the collecting holes have a collecting opening vertically above, and the collecting plate is arranged in the mounting groove; Features: The microporous filter plate negative pressure device also includes a bracket, a filter plate and a sealing cover, the bracket is provided with a through hole in the vertical direction, the bracket is provided with a support portion at the periphery of the through hole, the bracket is arranged in the mounting groove, and the outer wall of the bracket is connected to the inner peripheral wall; The filter plate is provided with a plurality of sample addition holes, each of the sample addition holes has a sample addition port vertically above, and each of the sample addition holes has a sample outlet vertically below, the filter plate is provided with a positioning portion on the periphery of the plurality of sample addition holes, the filter plate is installed in the through hole, the support portion is connected to the positioning portion, and one of the sample outlets is connected to one of the collection ports; The bottom surface of the sealing cover is provided with a hole sealing portion in the middle, and the bottom surface of the sealing cover is provided with an edge sealing portion at the periphery of the hole sealing portion. The sealing cover covers the installation port, the hole sealing portion tightly covers the multiple sample injection ports, and the edge sealing portion tightly covers the upper end surface of the bracket and the edge of the installation port.

2. The microporous filter plate negative pressure device according to claim 1, characterized in that: The hole sealing part includes a plurality of sealing convex columns, and sealing grooves are arranged between the plurality of sealing convex columns. One sealing convex column is inserted into one of the sample adding ports, and the edge of the sample adding port is located in the sealing groove.

3. The microporous filter plate negative pressure device according to claim 1, characterized in that: The edge sealing portion includes an inner ring, an outer ring, an annular groove and an outer edge, the outer ring is located at the outer circumference of the inner ring, the outer ring, the inner ring and the bottom surface of the sealing cover form the annular groove, the outer edge is located at the outer circumference of the outer ring, the upper end surface of the bracket is located in the annular groove and adjacent to the bottom surface of the sealing cover, the inner ring and the outer ring are adjacent to the bracket respectively, and the outer edge is adjacent to the edge of the mounting port.

4. The microporous filter plate negative pressure device according to claim 3, characterized in that: The inner peripheral wall is provided with a first supporting step, and the bracket is arranged on the first supporting step.

5. The microporous filter plate negative pressure device according to claim 3, characterized in that: The supporting portion is arranged in the form of a second supporting step, the positioning portion is arranged in the form of a ring platform, and the ring platform is arranged on the second supporting step.

6. The microporous filter plate negative pressure device according to claim 5, characterized in that: The plurality of sample addition holes all pass through the through hole.

7. The microporous filter plate negative pressure device according to claim 6, characterized in that: A positioning ring is provided on the periphery of the sample outlet, and the positioning ring is located in the collecting hole and is in clearance fit with the collecting hole.

8. The microporous filter plate negative pressure device according to any one of claims 1 to 7, characterized in that: The upper end surface of the bracket is provided with a handle protrusion, and the sealing cover is provided with a through groove, and the handle protrusion passes through the through groove.

9. The microporous filter plate negative pressure device according to claim 8, characterized in that: The upper end surface of the bracket is provided with two handle protrusions, and the two handle protrusions are respectively located on two horizontally opposite sides of the through hole. The sealing cover is penetrated by two through grooves, and one handle protrusion passes through one through groove.

10. The microporous filter plate negative pressure device according to any one of claims 1 to 7, characterized in that: The sealing cover is made of silicone material.

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