Microfluidic chip observation constant-temperature heating stage
By designing a microfluidic chip observation constant temperature heat table including transparent I TO electric heating glass and a two-dimensional adjustment slide platform, the problem of insufficient temperature control accuracy and inconvenient observation of microfluidic chips in the prior art is solved, and the precise temperature control and high-precision position control of microfluidic chips are achieved, which improves the efficiency and accuracy of the experiment.
Patent Information
- Application Number
- CN202421865956.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-02
- Publication Date
- 2025-05-13
- Estimated Expiration
- 2034-08-02
AI Technical Summary
The existing microfluidic chip experimental bench has insufficient temperature control accuracy and is inconvenient to cooperate with microscope devices, making it difficult to achieve uniform heating and convenient observation of microfluidic chips.
A microfluidic chip observation constant temperature heat table is designed, including a light source base, a rotating table, a two-dimensional adjustment sliding table assembly and a constant temperature heat table. Transparent I TO electric heating glass is used as a heating plate, combining a two-dimensional adjustment slide table and a rotary table to achieve accurate temperature control and high-precision position control of the microfluidic chip.
Accurate temperature control and high-precision position control of microfluidic chips are achieved, which avoids the temperature gradient problems caused by traditional heating devices, and is easy to use with microscope devices, improving the efficiency and accuracy of experiments.
Smart Images
Figure CN222855509U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidics, in particular to a microfluidics chip observation constant temperature hot stage. Background Art
[0002] As an emerging experimental technology, microfluidic chip technology has been widely used in biology, chemistry, medicine and other fields. Microfluidic chips usually have built-in tiny fluid channels (fluid channels as low as micrometers), which can accommodate reaction materials. The process and results of the experiment need to be observed or displayed by a microscope. The microfluidic chip also needs to be heated during the experiment to reach the experimental temperature. Existing microfluidic chip test benches are difficult to meet experimental requirements, which is mainly reflected in the following aspects:
[0003] 1: Insufficient temperature control accuracy: Traditional heating devices, such as electric heating plates, are difficult to achieve accurate temperature control of microfluidic chips. Due to the small size of microfluidic chips and uneven heat distribution, it is easy to cause a large temperature gradient, affecting the experimental results. For example, when the electric heating plate is heated, the temperature in the center area is higher and the temperature in the edge area is lower, making it difficult to ensure the uniformity of the temperature of the entire chip.
[0004] 2. Inconvenient to use with microscopes: Traditional heating devices are often large in size and difficult to use with microscopes, which makes experimental operations inconvenient and observation and analysis difficult. For example, placing a hot plate under a microscope will block part of the field of view and affect observation.
[0005] 3. Inconvenience in observing microfluidic chips: Researchers sometimes need to adjust the position to view local details. Before the experiment, the position will be adjusted so that the central axis of the microfluidic chip is as coaxial as possible with the field of view to facilitate subsequent image processing. Therefore, the bottom adjustment structure is very necessary. Utility Model Content
[0006] In view of this, in order to improve the temperature accuracy of microfluidic chip experiments and make the microfluidic chip easier to observe; the utility model provides a microfluidic chip observation constant temperature hot stage, including a light source base, a rotating stage, a two-dimensional adjustment slide assembly and a constant temperature hot stage;
[0007] The light source base comprises a base plate and a light source arranged on the base;
[0008] The rotating platform comprises a rotating base and a rotating disk, the rotating base is fixed to the base plate, the rotating disk is rotatably arranged on the rotating base, and the rotating base is also provided with a rotating driving mechanism for driving the rotating base;
[0009] The two-dimensional adjustment slide assembly is fixed on the rotating disk; the constant temperature hot stage is fixed on the two-dimensional adjustment slide assembly, and the two-dimensional adjustment slide assembly is used to adjust the position of the constant temperature hot stage in a two-dimensional plane;
[0010] The constant temperature hot stage is provided with a clamping spring and a transparent heating plate, the microfluidic chip to be observed is supported on the transparent heating plate, and the clamping spring presses the microfluidic chip.
[0011] Furthermore, the transparent heating plate is ITO electrically heated glass.
[0012] Furthermore, the constant temperature hot stage also includes a fixed frame, the ITO electric heating glass is embedded in the middle of the fixed frame, a clamping seat is provided on the fixed frame, one end of the clamping spring is connected to the clamping seat, and the other end presses the microfluidic chip.
[0013] Furthermore, the two-dimensional adjustment slide assembly is provided with two positioning columns, the bottom of the fixed frame is provided with two positioning holes, and the two positioning columns are respectively located in the two positioning holes.
[0014] Furthermore, the two-dimensional adjustment slide assembly includes two adjustment slides arranged relatively up and down, each adjustment slide includes a fixed connecting seat and a sliding seat, the sliding seat is slidably arranged on the fixed connecting seat, an adjustment block is provided on the side of the fixed connecting seat, an adjustment threaded hole is provided on the adjustment block, an adjustment screw is provided in the adjustment threaded hole, a fixed block is provided on the edge of the sliding seat, and the end of the adjustment screw is rotatably connected to the fixed block.
[0015] Furthermore, the sliding grooves on the two fixed connection seats are perpendicular to each other.
[0016] Furthermore, the two adjustment slides are respectively a transverse adjustment slide and a longitudinal adjustment slide, wherein the fixed connection seat of the transverse adjustment slide is fixed on the rotating disk, and the fixed connection seat of the longitudinal adjustment slide is fixed on the sliding seat of the transverse adjustment slide.
[0017] Furthermore, the rotation drive mechanism includes a drive motor, a worm and a worm wheel, the worm wheel is arranged below the rotating disk and fixedly connected to the rotating disk, the worm is arranged on the side of the rotating base, the worm is meshed with the worm wheel, and the end of the worm is connected to the drive motor.
[0018] Furthermore, the driving motor is fixed to the side surface of the rotating base through a motor mounting seat.
[0019] Furthermore, an adjusting knob is provided at the end of the adjusting screw away from the fixing block.
[0020] The utility model provides a microfluidic chip observation constant temperature hot stage with beneficial effects as follows: the constant temperature hot stage comprises a light source base, a rotating stage, a two-dimensional adjustment slide assembly and a constant temperature hot stage; a light source is provided on the light source base; the rotating stage comprises a rotating base and a rotating disk, and the rotating base is also provided with a rotating driving mechanism for driving the rotating base; the two-dimensional adjustment slide assembly is fixed on the rotating disk; the constant temperature hot stage is fixed on the two-dimensional adjustment slide assembly, and a clamping spring and a transparent heating plate are provided on the constant temperature hot stage, the microfluidic chip to be observed is supported on the transparent heating plate, and the clamping spring and the transparent heating plate are provided on the constant temperature hot stage. The microfluidic chip is pressed tightly. When conducting a microfluidic experiment, the transparent heating plate can heat the microfluidic chip attached to it as a whole to ensure that the microfluidic chip is heated evenly; the light source on the light source base irradiates the microfluidic chip fixed on the transparent heating plate from the bottom of the transparent heating plate upward, so that it can be observed by a microscope, avoiding the problem of traditional heating devices blocking the field of view; the rotating table and two-dimensional adjustment slide assembly can adjust the position of the microfluidic chip in a two-dimensional plane and adjust the layout angle of the microfluidic chip, which can improve the convenience of the experimental process and observation and reduce the difficulty of microfluidic experiments. The constant temperature hot stage adopts ITO glass heating and bottom displacement adjustment technology to achieve precise temperature control and high-precision position control of the microfluidic chip, effectively improving the efficiency and accuracy of microfluidic experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 The utility model is a schematic diagram of the dispersed structure of a microfluidic chip observation constant temperature hot stage.
[0022] Figure 2 The utility model is a schematic diagram of the connection structure of an adjusting screw and a fixing block of a microfluidic chip observation constant temperature hot stage.
[0023] In the above figure: 1-base plate, 11-light source, 2-rotating base, 21-rotating disk, 3-driving motor, 31-motor mounting seat, 4-adjusting slide, 41-fixed connecting seat, 42-sliding groove, 43-sliding seat, 44-adjusting block, 45-fixed block, 451-limiting cover, 46-adjusting screw, 461-connecting block, 47-adjusting knob, 48-positioning column, 5-fixed frame, 51-transparent heating plate, 52-clamping seat, 6-clamping spring. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical solution and advantages of the utility model clearer, the implementation mode of the utility model will be further described below in conjunction with the accompanying drawings.
[0025] Please refer to Figure 1 and Figure 2 The utility model discloses a microfluidic chip observation constant temperature hot stage, which comprises a light source base, a rotating stage 21, a two-dimensional adjustment slide assembly and a constant temperature hot stage.
[0026] The light source base includes a base plate 1 and a light source 11 arranged on the base; the base plate 1 is a rectangular plate, and the light source 11 is fixed in the middle of the base plate 1. The rotating table includes a rotating base 2 and a rotating disk 21, the rotating base 2 is fixed on the base plate 1, the rotating disk 21 is rotatably arranged on the rotating base 2, and the rotating base 2 is also provided with a rotating driving mechanism for driving the rotating base 2. The middle part of the rotating base 2 and the rotating disk 21 is a hollow structure, which is convenient for the light source 11 to irradiate upward. The two-dimensional adjustment slide assembly is fixed on the rotating disk 21; the constant temperature hot stage is fixed on the two-dimensional adjustment slide assembly, and the two-dimensional adjustment slide assembly is used to adjust the position of the constant temperature hot stage in a two-dimensional plane.
[0027] The constant temperature hot stage is provided with a clamping spring 6 and a transparent heating plate 51, the microfluidic chip to be observed is supported on the transparent heating plate 51, and the clamping spring 6 presses the microfluidic chip. In this embodiment, the transparent heating plate 51 is an ITO electric heating glass. The principle of ITO heating glass is to use electric current to pass through the heating element to generate heat, so that the surface temperature of the glass is increased as a whole, thereby achieving the purpose of uniform heating of the microfluidic chip. The constant temperature hot stage also includes a fixed frame 5, the ITO electric heating glass is embedded in the middle of the fixed frame 5, and a clamping seat 52 is provided on the fixed frame 5. One end of the clamping spring 6 is connected to the clamping seat 52, and the other end presses the microfluidic chip.
[0028] When conducting a microfluidic experiment, the transparent heating plate (ITO electric heating glass) of the constant temperature hot stage can heat the microfluidic chip attached to it as a whole, ensuring that the microfluidic chip is heated evenly; the light source on the light source base irradiates the microfluidic chip fixed on the transparent heating plate 51 from the bottom of the transparent heating plate 51 upward, so that it can be observed by a microscope, avoiding the problem of traditional instant heating devices blocking the field of view; the rotating stage and the two-dimensional adjustment slide assembly can adjust the position of the microfluidic chip in a two-dimensional plane and adjust the arrangement angle of the microfluidic chip, which can improve the convenience of the experimental process and observation and reduce the difficulty of the microfluidic experiment. The constant temperature hot stage adopts ITO glass heating and bottom displacement adjustment technology to achieve precise temperature control and high-precision position control of the microfluidic chip, effectively improving the efficiency and accuracy of the microfluidic experiment.
[0029] Preferably, the two-dimensional adjustment slide assembly includes two adjustment slides 4 relatively arranged up and down, each adjustment slide 4 includes a fixed connection seat 41 and a sliding seat 43, the sliding seat 43 is slidably arranged on the fixed connection seat 41, an adjustment block 44 is provided on the side of the fixed connection seat 41, an adjustment threaded hole is provided on the adjustment block 44, an adjustment screw 46 is provided in the adjustment threaded hole, a fixed block 45 is provided on the edge of the sliding seat 43, and the end of the adjustment screw 46 is rotatably connected to the fixed block 45. Specifically, a rotation connection hole and a limit cover 451 are provided on the fixed block 45, and a connection block 461 with a cylindrical structure is provided at the end of the adjustment screw 46, and the connection block 461 is rotatably arranged in the rotation connection hole. The limit cover 451 is connected to the connection block 461 by screws, and the connection block 461 is limited in the rotation connection hole, so that the end of the adjustment screw 46 is rotatably connected to the fixed block 45.
[0030] The two adjustment slides 4 are respectively a transverse adjustment slide and a longitudinal adjustment slide, wherein the fixed connection seat 41 of the transverse adjustment slide is fixed on the rotating disk, and the fixed connection seat 41 of the longitudinal adjustment slide is fixed on the sliding seat of the transverse adjustment slide. The sliding grooves 42 on the two fixed connection seats 41 are perpendicular to each other, and the end of the adjustment screw 46 away from the fixed block 45 is also provided with an adjustment knob 47.
[0031] When the position of the constant temperature hot plate needs to be adjusted, the adjustment screw 46 on the horizontal adjustment slide or the vertical adjustment slide can be rotated to drive the fixed block 45 to move along the direction of the sliding groove 42 on the fixed connection seat 41 where it is located. The two sliding grooves 42 are perpendicular to each other, so that the two-dimensional adjustment slide assembly can adjust the position of the constant temperature hot plate in a two-dimensional plane. It can be understood that the middle part of the two adjustment slides 4 is also a hollow structure, so that the light source 11 on the base plate 1 can illuminate the microfluidic chip, which is convenient for the microscope to observe the microfluidic chip.
[0032] Preferably, two positioning columns 48 are provided on the two-dimensional adjustment slide assembly, and the two positioning columns 48 are located on the sliding seat of the longitudinal adjustment slide. Two positioning holes are provided at the bottom of the fixed frame 5, and the two positioning columns 48 are respectively located in the two positioning holes. The positioning columns 48 and the positioning hole structure ensure that the fixed frame 5 is relatively fixed relative to the sliding seat of the longitudinal adjustment slide.
[0033] Preferably, the rotation drive mechanism includes a drive motor 3, a worm and a worm wheel (neither the worm nor the worm wheel is shown in the figure), and the drive motor 3 is fixed to the side of the rotating base 2 through a motor mounting seat 31. The worm wheel is arranged below the rotating disk and is fixedly connected to the rotating disk 21, the worm is arranged on the side of the rotating base 2, the worm is meshed with the worm wheel, and the end of the worm is connected to the drive motor 3. The drive motor 3 can drive the rotating disk 21 to rotate relative to the rotating base 2 through the worm and worm wheel matching structure, thereby adjusting the observation angle of the microfluidic chip.
[0034] In this document, the directional words such as front, back, top, and bottom are defined by the positions of the components in the drawings and the positions of the components relative to each other, and are only for the sake of clarity and convenience in expressing the technical solution. It should be understood that the use of the directional words should not limit the scope of protection claimed in this application.
[0035] In the absence of conflict, the above embodiments and features in the embodiments may be combined with each other.
[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A microfluidic chip observation constant temperature hot stage, characterized in that: It includes a light source base, a rotating stage, a two-dimensional adjustment slide assembly and a constant temperature hot stage; The light source base comprises a base plate and a light source arranged on the base plate; The rotating platform comprises a rotating base and a rotating disk, the rotating base is fixed to the base plate, the rotating disk is rotatably arranged on the rotating base, and the rotating base is also provided with a rotating driving mechanism for driving the rotating base; The two-dimensional adjustment slide assembly is fixed on the rotating disk; the constant temperature hot stage is fixed on the two-dimensional adjustment slide assembly, and the two-dimensional adjustment slide assembly is used to adjust the position of the constant temperature hot stage in a two-dimensional plane; The constant temperature hot stage is provided with a clamping spring and a transparent heating plate, the microfluidic chip to be observed is supported on the transparent heating plate, and the clamping spring presses the microfluidic chip.
2. A microfluidic chip observation constant temperature hot stage according to claim 1, characterized in that: The transparent heating plate is ITO electrically heated glass.
3. A microfluidic chip observation constant temperature hot stage according to claim 2, characterized in that: The constant temperature hot stage also includes a fixed frame, the ITO electric heating glass is embedded in the middle of the fixed frame, a clamping seat is provided on the fixed frame, one end of the clamping spring is connected to the clamping seat, and the other end presses the microfluidic chip.
4. A microfluidic chip observation constant temperature hot stage according to claim 3, characterized in that: The two-dimensional adjustment slide assembly is provided with two positioning columns, the bottom of the fixed frame is provided with two positioning holes, and the two positioning columns are respectively located in the two positioning holes.
5. A microfluidic chip observation constant temperature hot stage according to claim 3, characterized in that: The two-dimensional adjustment slide assembly includes two adjustment slides arranged relatively up and down, each adjustment slide includes a fixed connection seat and a sliding seat, the sliding seat is slidably arranged on the fixed connection seat, an adjustment block is provided on the side of the fixed connection seat, an adjustment threaded hole is provided on the adjustment block, an adjustment screw is provided in the adjustment threaded hole, a fixed block is provided on the edge of the sliding seat, and the end of the adjustment screw is rotatably connected to the fixed block.
6. A microfluidic chip observation constant temperature hot stage according to claim 5, characterized in that: The sliding grooves on the two fixed connection seats are perpendicular to each other.
7. A microfluidic chip observation constant temperature hot stage according to claim 5, characterized in that: The two adjustment slides are respectively a transverse adjustment slide and a longitudinal adjustment slide, wherein the fixed connection seat of the transverse adjustment slide is fixed on the rotating disk, and the fixed connection seat of the longitudinal adjustment slide is fixed on the sliding seat of the transverse adjustment slide.
8. A microfluidic chip observation constant temperature hot stage according to claim 1, characterized in that: The rotary drive mechanism includes a drive motor, a worm and a worm wheel. The worm wheel is arranged below the rotating disk and fixedly connected to the rotating disk. The worm is arranged on the side of the rotating base. The worm is meshed with the worm wheel. The end of the worm is connected to the drive motor.
9. A microfluidic chip observation constant temperature hot stage according to claim 8, characterized in that: The driving motor is fixed to the side surface of the rotating base through a motor mounting seat.
10. A microfluidic chip observation constant temperature hot stage according to claim 5, characterized in that: An adjusting knob is also provided at the end of the adjusting screw away from the fixing block.