Sterile sampling culture bottle

By using the combination of L-shaped limiting rods and limiting slots in the culture bottle, the limit fixation of the bottle cap is achieved, and the baffle and activated carbon plate are used for sealing and breathable adjustment, the pollution problem caused by improper selection or looseness of the bottle cap after sterile sampling of the existing culture bottle is solved, ensuring the cultivation effect and the purity of the substance.

CN222877949UActive Publication Date: 2025-05-16上海酵诚生物技术有限公司
View PDF 0 Cites 0 Cited by

Patent Information

Application Number
CN202421750616.6
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-23
Publication Date
2025-05-16
Estimated Expiration
2034-07-23

AI Technical Summary

Technical Problem

After sterile sampling of existing culture bottles, improper selection of bottle caps or looseness leads to culture contamination, affecting the experimental results.

Method used

A sterile sampling culture bottle is designed, using a combination of an L-shaped limiting rod and limiting groove, and the limiting fixation of the bottle cap is achieved through tensioning springs and moving blocks, and sealing and breathable adjustment is made using baffles and activated carbon plates.

Benefits of technology

It effectively avoids loosening and contamination of the bottle cap, ensures the culture effect, and filters harmful substances through activated carbon plates to protect cells or tissues in the culture medium.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN222877949U_ABST
    Figure CN222877949U_ABST
Patent Text Reader

Abstract

The utility model discloses a sterile sampling culture bottle, and relates to the technical field of culture bottles. A sterile sampling culture bottle comprises a bottle body, a bottle cap is arranged at the position of a bottle opening of the bottle body, limiting grooves are circumferentially formed in the bottle cap at equal intervals, an L-shaped limiting rod is arranged on one side of the bottle cap and located in the limiting grooves, a limiting plate is arranged on the bottle body, a sliding groove is formed in the limiting plate, and the sliding groove is located in the bottle body. A sliding groove is formed in the limiting plate, a limiting block is connected in the sliding groove in a sliding mode, the limiting block is connected with the limiting plate through a tensioning spring, a moving block is arranged at the upper end of the limiting block, the L-shaped limiting rod penetrates through the limiting plate, and a first inserting groove used in cooperation with the limiting block is formed in the L-shaped limiting rod. The bottle cap is limited and fixed through the L-shaped limiting rod, switching between sealed culture and breathable culture is achieved through the baffle, culture is prevented from being polluted, and the culture effect is effectively guaranteed.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The utility model belongs to the technical field of culture bottles, and specifically relates to an aseptic sampling culture bottle. Background Art

[0002] The in vitro culture of cells and tissues has become an indispensable part of life research and practice. The culture flask is the main instrument for cell and tissue culture. Cells or tissues are inoculated into the liquid culture medium in the culture flask for culture.

[0003] When the existing culture bottles are cultured after aseptic sampling, it is necessary to select a breathable bottle cap or a sealed bottle cap according to the type of cells or tissues to be cultured. The wrong bottle cap may affect the experiment. In addition, since the bottle cap and the bottle mouth are often connected by threads without limiting, the bottle cap and the bottle mouth are prone to looseness, the bottle cap may fall from the bottle mouth, and the culture material in the culture bottle is prone to contamination. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a sterile sampling culture bottle which can overcome the above problems or at least partially solve the above problems.

[0005] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is: an aseptic sampling culture bottle, comprising a bottle body, a bottle cap is arranged at the bottle mouth of the bottle body, and the bottle cap is provided with limiting grooves at equal intervals on the circumference, an L-shaped limiting rod is arranged on one side of the bottle cap, and the L-shaped limiting rod is located in the limiting groove, a limiting plate is arranged on the bottle body, a slide groove is arranged in the limiting plate, a limiting block is slidably connected in the slide groove, and the limiting block is connected to the limiting plate by a tensioning spring. A moving block is arranged at the upper end of the limit block, the L-shaped limit rod passes through the limit plate, a first slot used in conjunction with the limit block is provided on the L-shaped limit rod, a slot is provided on the L-shaped limit rod at the bottle cap, an activated carbon plate is provided in the slot, a moving slot is provided on the L-shaped limit rod above the activated carbon plate, the moving slot is connected with the slot, a baffle is slid in the moving slot, a pull rod is provided on the baffle, and an air hole is provided on the bottle cap below the activated carbon plate.

[0006] In order to limit the position of the bottle cap more stably, further, the L-shaped limiting rods are symmetrically arranged.

[0007] In order to avoid the loss of the L-shaped limiting rod, further, a second slot is provided on the L-shaped limiting rod below the first slot.

[0008] In order to prevent the baffle from moving freely, further, a first magnetic block is symmetrically arranged on the baffle, and a second magnetic block used in conjunction with the first magnetic block is arranged on the L-shaped limiting rod.

[0009] In order to facilitate sampling and testing of the culture in the culture bottle, further, a sampling port is opened on the bottle cap, and a sealing plug is arranged in the sampling port.

[0010] In order to facilitate breaking up the bacterial clumps, further, a plurality of collision blocks are arranged at the lower end of the inner part of the bottle body, and the bottle body is circumferentially evenly concave inward to form protrusions.

[0011] After adopting the above technical scheme, the utility model has the following beneficial effects compared with the prior art: compared with the existing sterile sampling culture bottle, the utility model limits and fixes the bottle cap through an L-shaped limiting rod, and uses a baffle to achieve the switching between sealed culture and breathable culture, thereby avoiding contamination of the culture and effectively ensuring the culture effect.

[0012] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In the attached picture:

[0014] Figure 1 This is a front view structural schematic diagram of a sterile sampling culture bottle proposed by the utility model;

[0015] Figure 2 A top view schematic diagram of a bottle cap in a sterile sampling culture bottle proposed by the utility model

[0016] Figure 3 A sterile sampling culture bottle proposed by the utility model Figure 1 The enlarged schematic diagram of point A in the middle;

[0017] Figure 4 A sterile sampling culture bottle proposed by the utility model Figure 1 A magnified schematic diagram of point B in the middle.

[0018] In the figure: 1, bottle body; 101, collision block; 2, bottle cap; 201, sealing plug; 3, L-shaped limit rod; 301, baffle; 302, activated carbon plate; 303, pull rod; 304, first magnetic block; 305, second magnetic block; 4, limit plate; 401, limit block; 402, tension spring; 403, moving block. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model but are not used to limit the scope of the utility model.

[0020] Embodiment 1:

[0021] Reference Figure 1-Figure 4 A sterile sampling culture bottle comprises a bottle body 1, a bottle cap 2 is arranged at the bottle mouth of the bottle body 1, and the bottle cap 2 is provided with limiting grooves at equal intervals on the circumference, an L-shaped limiting rod 3 is arranged on one side of the bottle cap 2, and the L-shaped limiting rod 3 is located in the limiting groove, a limiting plate 4 is arranged on the bottle body 1, and a slide groove is arranged in the limiting plate 4, and a limiting block 401 is slidably connected in the slide groove, and the limiting block 401 is connected to the limiting plate 4 by a tensioning spring 402, and a moving block 403 is arranged on the upper end of the limiting block 401, L The L-shaped limiting rod 3 penetrates the limiting plate 4, a first slot for use with the limiting block 401 is provided on the L-shaped limiting rod 3, a slot is provided on the L-shaped limiting rod 3 at the bottle cap 2, an activated carbon plate 302 is provided in the slot, a movable slot is provided on the L-shaped limiting rod 3 above the activated carbon plate 302, the movable slot is connected to the slot, a baffle 301 slides in the movable slot, a pull rod 303 is provided on the baffle 301, and an air hole is provided on the bottle cap 2 below the activated carbon plate 302;

[0022] The L-shaped limit rods 3 are symmetrically arranged;

[0023] The L-shaped limiting rod 3 is provided with a second slot below the first slot.

[0024] When it is necessary to culture bacteria or tissues, the sterile sampled bacteria or tissues are placed in the culture fluid of the culture bottle for culture, and then the bottle cap 2 is covered, and the moving block 403 is pulled to make the limit block 401 leave the second slot, and the L-shaped limit rod 3 moves down into the limit slot, and then the moving block 403 is released. Under the action of the tensioning spring 402, the limit block 401 moves into the first slot and limits the L-shaped limit rod 3, so that the L-shaped limit rod 3 limits the bottle cap 2 to prevent the bottle cap 2 from rotating. When the bottle cap 2 needs to be removed, the moving block 403 is pulled, and the L-shaped limit rod 3 is moved up to leave the limit slot, so that the second slot matches the limit block 401, and the limit block 401 enters the second slot. The L-shaped limit rod 3 is limited and fixed inside, and then the bottle cap 2 can be rotated to take it out, and when the sterile sampled bacteria or tissues are placed in the culture medium of the culture bottle for culture, the pull rod 303 can be pulled according to the culture requirements to move the baffle 301 for sealing and ventilation adjustment, and the wrong bottle cap 2 will not be selected, and the activated carbon plate 302 can be used to adsorb and filter harmful substances in the exchange gas to avoid contamination of cells or tissues in the culture medium. Compared with the existing sterile sampling culture bottles, the bottle cap 2 is limited and fixed by the L-shaped limit rod 3, and the baffle 301 is used to realize the switching between sealed culture and breathable culture, so as to avoid contamination of the culture and effectively ensure the culture effect.

[0025] Embodiment 2:

[0026] Reference Figure 1-Figure 4, a sterile sampling culture bottle, which is basically the same as Example 1, and further, a first magnetic block 304 is symmetrically arranged on the baffle 301, and a second magnetic block 305 used in conjunction with the first magnetic block 304 is arranged on the L-shaped limiting rod 3. The baffle 301 can be fixed by the close fit between the first magnetic block 304 and the second magnetic block 305 to prevent the movement of the baffle 301 from affecting the culture effect.

[0027] A sampling port is provided on the bottle cap 2, and a sealing plug 201 is arranged in the sampling port. When sampling is performed, only the sealing plug 201 needs to be pulled out to perform sampling, and there is no need to open the bottle cap 2, which makes sampling more convenient and avoids contamination of the culture solution when opening the bottle cap 2.

[0028] A plurality of bumps 101 are arranged at the lower end of the bottle body 1. The bottle body 1 is concave inward at equal intervals on the circumference to form bumps. During the bacterial culture process, when the bacteria reach a certain concentration, they will form clumps that are difficult to be shaken apart. The bumps 101 and the bumps are conducive to breaking up the bacterial clumps, so that the bacteria form uniform individuals, which is conducive to the growth of bacteria.

[0029] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment, it is not intended to limit the present invention.

Claims

1. A sterile sampling culture bottle, characterized in that: The invention comprises a bottle body (1), a bottle cap (2) is arranged at the bottle mouth of the bottle body (1), limiting grooves are arranged on the bottle cap (2) at equal intervals on the circumference, an L-shaped limiting rod (3) is arranged on one side of the bottle cap (2), the L-shaped limiting rod (3) is located in the limiting groove, a limiting plate (4) is arranged on the bottle body (1), a sliding groove is arranged in the limiting plate (4), a limiting block (401) is slidably connected in the sliding groove, the limiting block (401) is connected to the limiting plate (4) by a tensioning spring (402), a moving block (403) is arranged on the upper end of the limiting block (401), and the L-shaped limiting rod (3) is located in the limiting groove. The limiting rod (3) passes through the limiting plate (4); a first slot for use with the limiting block (401) is provided on the L-shaped limiting rod (3); a slot is provided on the L-shaped limiting rod (3) at the bottle cap (2); an activated carbon plate (302) is provided in the slot; a movable slot is provided on the L-shaped limiting rod (3) above the activated carbon plate (302); the movable slot is connected to the slot; a baffle (301) slides in the movable slot; a pull rod (303) is provided on the baffle (301); and an air hole is provided on the bottle cap (2) below the activated carbon plate (302).

2. A sterile sampling culture bottle according to claim 1, characterized in that: The L-shaped limiting rods (3) are symmetrically arranged.

3. A sterile sampling culture bottle according to claim 2, characterized in that: The L-shaped limiting rod (3) is provided with a second slot below the first slot.

4. A sterile sampling culture bottle according to claim 1, characterized in that: The baffle (301) is symmetrically provided with a first magnetic block (304), and the L-shaped limiting rod (3) is provided with a second magnetic block (305) used in conjunction with the first magnetic block (304).

5. A sterile sampling culture bottle according to claim 1, characterized in that: The bottle cap (2) is provided with a sampling port, and a sealing plug (201) is arranged in the sampling port.

6. A sterile sampling culture bottle according to claim 1, characterized in that: A plurality of collision blocks (101) are arranged at the lower end of the bottle body (1), and the bottle body (1) is concave inwardly at equal intervals in a circle to form protrusions.