Condensed protein gel electrophoresis device

Abstract

The utility model relates to the technical field of protein separation and detection, in particular to a condensed protein gel electrophoresis device which comprises an electrophoresis tank, a glue clamping frame and an upper cover, and further comprises a condensation pipe, a rotary drum and a first water pipe connector, the condensation pipe is parallel to the glue clamping frame and is fixedly arranged on the inner side wall of the electrophoresis tank, and the rotary drum is fixedly arranged on the outer side wall of the electrophoresis tank. The two ends of the condensation pipe horizontally penetrate through the side ends of the electrophoresis tank; the two rotary drums are arranged at the two ends of the condensation pipe respectively, one end of each rotary drum is rotationally arranged at the outer side end of the electrophoresis tank, the other end of each rotary drum is closed, and the two rotary drums are communicated through the condensation pipe; one first water pipe connector is in threaded connection with the side portion of each rotary drum, and the first water pipe connectors are used for being connected with a tap water pipe so that flowing water can be formed in the condensation pipe; according to the electrophoresis tank, the condensation pipe is arranged in the electrophoresis tank, so that electrophoresis liquid can be cooled conveniently, and the situation that the temperature of the electrophoresis liquid is too high in the electrophoresis process and influences experiment results is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of protein separation and detection, in particular to a condensation protein gel electrophoresis device. Background Art

[0002] Protein gel electrophoresis is a common method for separating and detecting proteins. It separates proteins into different strips by applying an electric field in a gel medium. These strips can be stained or further separated and identified. In protein gel electrophoresis, the gel medium acts as the stationary phase and the protein acts as the mobile phase. The gel medium is usually polyacrylamide or agarose. During electrophoresis, the ions in the gel medium move with the applied electric field, and the proteins are separated into different strips.

[0003] Generally, as the electrophoresis time increases or the applied electric field pressure is too large, a lot of heat will be generated, causing the temperature of the electrophoresis fluid to rise. Proteins are easily degraded at higher temperatures, resulting in the loss of some of the proteins to be tested. At the same time, when the electrophoresis temperature is too high, protein bands are easy to diffuse, deform, and even cause the gel to melt, ultimately affecting the experimental results. Therefore, when performing protein gel electrophoresis, it is crucial to ensure the stability of the electrophoresis fluid temperature for protein gel electrophoresis detection.

[0004] The utility model with publication number CN210109022U discloses a protein electrophoresis instrument, including an electrophoresis tank, a laminated frame, an upper cover, and a positive electrode plug and a negative electrode plug for connecting to an external power supply and supplying power for an electrophoresis test; the laminated frame is arranged on the outer laminated frame, and the outer laminated frame is arranged in the electrophoresis tank; the laminated frame is provided with a positive electrode plug and a negative electrode plug.

[0005] When the above-mentioned technical solution is used for protein gel electrophoresis, the temperature of the electrophoresis fluid in the electrophoresis tank will increase with the increase of electrophoresis time. The electrophoresis fluid in the high temperature state is prone to cause protein degradation in the tank, thereby affecting the experimental results. Utility Model Content

[0006] In view of this, the utility model proposes a condensation protein gel electrophoresis device, which arranges a condenser in the electrophoresis tank to cool the electrophoresis fluid, thereby ensuring that the temperature of the electrophoresis fluid will not be too high during the electrophoresis process to affect the experimental results.

[0007] The technical solution of the utility model is achieved in this way:

[0008] The utility model provides a condensation protein gel electrophoresis device, comprising an electrophoresis tank, a rubber-coated frame and an upper cover, wherein:

[0009] The rubber-clamping frame is detachably arranged at the middle position of the inner side of the electrophoresis tank;

[0010] The upper cover is buckled on the top of the electrophoresis tank;

[0011] It also includes a condenser, a rotating drum and a first water pipe joint, wherein:

[0012] The condenser tube is parallel to the rubber-clamped frame and is fixedly arranged on the inner wall of the electrophoresis tank, and both ends of the condenser tube horizontally penetrate the side ends of the electrophoresis tank;

[0013] The rotating drum is provided at each end of the condenser tube, one end of the rotating drum is rotatably provided on the outer end of the electrophoresis tank, and the other end is closed, and the two rotating drums are connected through the condenser tube;

[0014] The first water pipe joint is screwed on the side of each of the rotating drums, and is used to connect a tap water pipe so that flowing water is formed in the condenser.

[0015] On the basis of the above technical solution, preferably, the condenser is bent in a snake shape, and the cross-section of the condenser is a rectangular shape.

[0016] On the basis of the above technical solution, preferably, a first threaded hole is provided on the side of the rotating drum, and the first threaded hole is threadedly connected to one end of the first water pipe connector.

[0017] On the basis of the above technical solution, preferably, it further includes a first filter screen and a first end cover, wherein:

[0018] The first filter screen is disposed in each of the rotating drums, and the first filter screen and the corresponding rotating drum are disposed coaxially;

[0019] A second threaded hole is provided on one closed end of each of the rotating drums, the second threaded hole is coaxially arranged with the corresponding rotating drum, and the inner diameter of the second threaded hole is larger than the outer diameter of the first filter screen;

[0020] One of the first end caps is screwed onto each of the second threaded holes;

[0021] One end of the first filter screen is in contact with the side end of the electrophoresis tank, and the other end is in contact with the first end cover;

[0022] One end of the condensation tube is arranged on the inner side of the corresponding first filter screen.

[0023] On the basis of the above technical solution, preferably, two condensing tubes are provided, the two condensing tubes are mirror-symmetrical on both sides of the laminated frame, and one rotating drum is correspondingly provided at both ends of each condensing tube.

[0024] On the basis of the above technical solution, preferably, it further includes a water inlet pipe and a water outlet pipe, wherein:

[0025] The water inlet pipe and the water outlet pipe are both three-way pipes;

[0026] The two first water pipe joints at one end of the two condensing pipes are connected through the water inlet pipe, and the two first water pipe joints at the other end are connected through the water outlet pipe.

[0027] On the basis of the above technical solution, preferably, the first water pipe joint is detachably connected to the corresponding water inlet pipe and the water outlet pipe.

[0028] On the basis of the above technical solution, preferably, it further comprises a second water pipe joint, wherein:

[0029] A second water pipe joint is fixedly arranged on one end of the water inlet pipe and the water outlet pipe away from the first water pipe joint.

[0030] On the basis of the above technical solution, preferably, the water inlet pipe and the water outlet pipe are both hoses.

[0031] On the basis of the above technical solution, preferably, it further comprises a second filter screen and a second end cover, wherein:

[0032] The second filter is coaxially arranged in one of the drums, a third threaded hole is arranged on the closed end of the drum on which the second filter is arranged, the third threaded hole is coaxially arranged with the corresponding drum, and the inner diameter of the third threaded hole is larger than the outer diameter of the second filter;

[0033] One end of the second end cover is screwed into the third threaded hole;

[0034] One end of the second filter screen is in contact with the side end of the electrophoresis tank, and the other end is in contact with the second end cover;

[0035] One end of the condenser tube close to the second filter screen is a water inlet end, and the other end is a water outlet end. The water inlet end of the condenser tube is located on the inner side of the second filter screen.

[0036] The condensation protein gel electrophoresis device of the utility model has the following beneficial effects compared with the prior art:

[0037] (1) By installing a condenser in the electrophoresis tank, the electrophoresis fluid can be cooled to ensure that the temperature of the electrophoresis fluid will not be too high during the electrophoresis process and affect the experimental results.

[0038] (2) By providing the rotating drum and the first water pipe joint, it is convenient to adjust the direction of water inlet and outlet, thereby facilitating the installation of the water pipe.

[0039] (3) By setting the condenser tube to be bent in a snake shape and the cross-section of the condenser tube to be rectangular, the condenser tube is made relatively flat, thereby reducing the space occupied in the electrophoresis tank. In addition, the flat condenser tube has a larger heat exchange area, which can improve the cooling effect on the electrophoresis liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0041] Figure 1 It is an exploded view of a condensation protein gel electrophoresis device of the utility model;

[0042] Figure 2 It is a partial exploded view of the first embodiment of the utility model;

[0043] Figure 3 It is a partial top view of the first embodiment of the utility model;

[0044] Figure 4 For the utility model Figure 3 AA section view;

[0045] Figure 5 It is a partial exploded view of the second embodiment of the utility model;

[0046] In the figure: 1, electrophoresis tank; 2, laminated frame; 3, upper cover; 4, condenser; 5, rotating drum; 6, first water pipe joint; 7, first filter screen; 8, first end cover; 9, water inlet pipe; 10, water outlet pipe; 11, second water pipe joint; 12, second filter screen; 13, second end cover; 501, first threaded hole; 502, second threaded hole; 503, third threaded hole. DETAILED DESCRIPTION

[0047] The following will be combined with the specific implementation of the utility model to clearly and completely describe the technical solution in the utility model. Obviously, the described implementation is only a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0048] Embodiment 1: Figure 1-4As shown, a condensation protein gel electrophoresis device of the utility model comprises an electrophoresis tank 1, a rubber-coated frame 2 and an upper cover 3, and also comprises a condensation tube 4, a rotating drum 5 and a first water pipe joint 6.

[0049] Among them, the laminated frame 2 is detachably arranged in the middle position of the inner side of the electrophoresis tank 1, and the upper cover 3 is buckled on the top of the electrophoresis tank 1. The laminated frame 2 and the upper cover 3 are any one of the prior art, such as a protein electrophoresis instrument disclosed in CN210109022U. The specific structure and operation and use of the laminated frame 2 and the upper cover 3 are not repeated.

[0050] The condenser 4 is parallel to the laminated frame 2 and is fixedly arranged on the inner wall of the electrophoresis tank 1. Both ends of the condenser 4 horizontally penetrate the side ends of the electrophoresis tank 1. Tap water is transported through the condenser 4. The flowing tap water exchanges heat with the electrophoresis liquid in the electrophoresis tank 1, so that the electrophoresis liquid in the electrophoresis is cooled, which is beneficial to the protection of the protein. Specifically, the condenser 4 is bent in a snake shape, one end of which is used for water inlet and the other end is used for water outlet. The cross-section of the condenser 4 is a rectangular shape. This shape makes the condenser 4 relatively flat, reducing the space occupied in the electrophoresis tank 1, and the flat condenser 4 has a larger heat exchange area, which can improve the cooling effect on the electrophoresis liquid.

[0051] To facilitate the connection of the tap water pipe, a rotating drum 5 is provided at each end of the condenser tube 4. One end of the rotating drum 5 is rotatably provided on the outer end of the electrophoresis tank 1, and the other end is closed. The two rotating drums 5 are connected through the condenser tube 4. A first water pipe joint 6 is screwed on the side of each rotating drum 5. The first water pipe joint 6 is used to connect the tap water pipe so that flowing water is formed in the condenser tube 4. During electrophoresis, the water outlet of the tap water pipe is connected to the first water pipe joint 6 on one of the rotating drums 5 through a pipe, and the first water pipe joint 6 on the other rotating drum 5 is connected to the sewage pipe through a pipe. Water is injected into the condenser tube 4 through tap water and the water flows in the condenser tube 4. The water flowing through the condenser tube 4 exchanges heat with the electrophoresis liquid and then enters the sewage pipe through the pipe.

[0052] like Figure 2 As shown, in the above structure, a first threaded hole 501 is provided on the side of the rotating drum 5 , and the first threaded hole 501 is threadedly connected to one end of the first water pipe connector 6 , so as to facilitate the installation of the first water pipe connector 6 .

[0053] Under unobstructed conditions, tap water may contain impurities, such as scale. To prevent the impurities from clogging the condenser tube 4, the condensation protein gel electrophoresis device further includes a first filter screen 7 and a first end cover 8.

[0054] Among them, Figure 2As shown, a first filter screen 7 is arranged in each rotating drum 5, and the first filter screen 7 and the corresponding rotating drum 5 are arranged coaxially; a second threaded hole 502 is arranged on the closed end of each rotating drum 5, and the second threaded hole 502 is arranged coaxially with the corresponding rotating drum 5, and the inner diameter of the second threaded hole 502 is larger than the outer diameter of the first filter screen 7; a first end cover 8 is screwed on each second threaded hole 502; one end of the first filter screen 7 is in contact with the side end of the electrophoresis tank 1, and the other end is in contact with the first end cover 8; one end of the condenser 4 is arranged on the inner side of the corresponding first filter screen 7; when tap water is injected into the corresponding rotating drum 5, impurities in the water are intercepted by the first filter screen 7 in the rotating drum 5, so that the water entering the condenser 4 is relatively clean. Since the first filter screens 7 are arranged in the rotating drums 5 at both ends of the condenser 4, the two rotating drums 5 at both ends of the condenser 4 can be used as water inlet ends or water outlet ends.

[0055] In the above structure, after the first end cover 8 is disassembled, the first filter screen 7 can be taken out, thereby facilitating the cleaning of the first filter screen 7. In addition, when one of the drums 5 is used as a water outlet, the first filter screen 7 in the drum 5 can be taken out to improve the drainage effect.

[0056] In addition, to prevent water leakage, a countersunk hole is provided on the side end of the electrophoresis tank 1 at a position corresponding to the rotating drum 5, and one end of the rotating drum 5 is rotatably arranged in the countersunk hole, and a dynamic seal is provided between the countersunk hole and the rotating drum 5, so that the rotating drum 5 can rotate on the countersunk hole, and there will be no water leakage at the matching surface of the rotating drum 5 and the countersunk hole. The dynamic seal is any rotary dynamic seal in the prior art, such as a labyrinth dynamic seal. The labyrinth seal refers to a seal with many tortuous small chambers between the rotating parts and the fixed parts to reduce leakage, that is, a number of annular sealing teeth arranged in sequence are provided around the rotating drum 5, and a series of intercepting gaps and expansion cavities are formed between the teeth. When the sealed medium passes through the gaps in the tortuous labyrinth, a throttling effect is produced to achieve the purpose of preventing leakage.

[0057] Furthermore, in order to improve the cooling effect, Figure 3 As shown, two condensing tubes 4 are provided, and the two condensing tubes 4 are mirror-symmetrical on both sides of the laminated frame 2 , and a rotating drum 5 is correspondingly provided at both ends of each condensing tube 4 .

[0058] In order to facilitate the simultaneous injection of water into the two condensing tubes 4, the above-mentioned condensing protein gel electrophoresis device further includes a water inlet pipe 9 and a water outlet pipe 10, wherein Figure 1 As shown, the water inlet pipe 9 and the water outlet pipe 10 are both three-way pipes; the two first water pipe joints 6 at one end of the two condensers 4 are connected through the water inlet pipe 9, and the two first water pipe joints 6 at the other end are connected through the water outlet pipe 10; the water inlet pipe 9 and the water outlet pipe 10 are used to achieve simultaneous water inflow and drainage of the two condensers 4.

[0059] In the above structure, the first water pipe joint 6 is detachably connected to the corresponding water inlet pipe 9 and water outlet pipe 10, that is, the first water pipe joint 6 is any quick-change joint in the prior art, such as a gas line quick-change joint, and one end of the water inlet pipe 9 and the water outlet pipe 10 are plugged and fixed in the corresponding first water pipe joint 6.

[0060] In addition, the water inlet pipe 9 and the water outlet pipe 10 are both hoses, and a second water pipe joint 11 is fixedly provided on one end of the water inlet pipe 9 and the water outlet pipe 10 away from the first water pipe joint 6. The second water pipe joint 11 is also any quick-change joint in the prior art, and the tap water pipe can be conveniently connected through the second water pipe joint 11.

[0061] Embodiment 2: Figure 5 As shown, the above-mentioned condensation protein gel electrophoresis device further includes a second filter screen 12 and a second end cover 13. Compared with the first embodiment, the first filter screen 7 and the first end cover 8 are not provided in this embodiment.

[0062] like Figure 5 As shown, at each condenser 4, the second filter screen 12 is coaxially arranged in one of the drums 5, and a third threaded hole 503 is arranged on the closed end of the drum 5 provided with the second filter screen 12. The third threaded hole 503 and the corresponding drum 5 are coaxially arranged, and the inner diameter of the third threaded hole 503 is larger than the outer diameter of the second filter screen 12; one end of the second end cover 13 is screwed into the third threaded hole 503; one end of the second filter screen 12 is in contact with the side end of the electrophoresis tank 1, and the other end is in contact with the second end cover 13; one end of the condenser 4 close to the second filter screen 12 is the water inlet end, and the other end is the water outlet end, and the water inlet end of the condenser 4 is on the inner side of the second filter screen 12.

[0063] like Figure 5 As shown, the end above the two condensing tubes 4 is the water inlet end, and a second filter screen 12 is arranged in the rotating drum 5 at this end. The end below the two condensing tubes 4 is the water outlet end, and the end of the rotating drum 5 at this end away from the electrophoresis tank 1 is in a closed state.

[0064] The method for using the condensation protein gel electrophoresis device of the utility model is as follows:

[0065] First, the first water pipe joint 6 at the water inlet end of the condenser 4 is connected to the tap water pipe through the water inlet pipe 9, and the first water pipe joint 6 at the water outlet end of the condenser 4 is connected to the sewage pipe through the water outlet pipe 10 to establish an effective circulating water circuit.

[0066] During the electrophoresis operation stage, the tap water supply is turned on, and the water will flow into the condenser 4 through the water inlet pipe 9 to form a circulation, and perform heat exchange with the electrophoresis liquid in the electrophoresis tank 1, thereby achieving continuous cooling of the electrophoresis liquid, and the water after heat exchange flows into the sewage pipe.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A condensation protein gel electrophoresis device, comprising an electrophoresis tank (1), a laminated frame (2) and an upper cover (3), wherein: The rubber-clamping frame (2) is detachably arranged at the middle position of the inner side of the electrophoresis tank (1); The upper cover (3) is buckled onto the top of the electrophoresis tank (1); It is characterized in that it also includes a condenser (4), a rotating drum (5) and a first water pipe joint (6), wherein: The condenser tube (4) is parallel to the laminated frame (2) and is fixedly arranged on the inner wall of the electrophoresis tank (1), and both ends of the condenser tube (4) horizontally penetrate the side ends of the electrophoresis tank (1); The rotating drum (5) is provided at each end of the condenser tube (4), one end of the rotating drum (5) is rotatably arranged on the outer end of the electrophoresis tank (1), and the other end is closed, and the two rotating drums (5) are connected through the condenser tube (4); The first water pipe joint (6) is screwed onto the side of each of the rotating drums (5), and the first water pipe joint (6) is used to connect a tap water pipe so that flowing water is formed in the condenser tube (4).

2. A cryogel electrophoresis apparatus according to claim 1, characterized in that: The condenser tube (4) is bent in a snake shape, and the cross section of the condenser tube (4) is a rectangular shape.

3. A cryogel electrophoresis apparatus according to claim 2, characterized in that: A first threaded hole (501) is provided on the side of the rotating drum (5), and the first threaded hole (501) is threadedly connected to one end of the first water pipe joint (6).

4. A cryogel electrophoresis apparatus according to claim 3, characterized in that: It also includes a first filter screen (7) and a first end cover (8), wherein: One of the first filter screens (7) is disposed in each of the rotating drums (5), and the first filter screen (7) and the corresponding rotating drum (5) are coaxially disposed; A second threaded hole (502) is provided on one closed end of each rotating cylinder (5), the second threaded hole (502) and the corresponding rotating cylinder (5) are coaxially arranged, and the inner diameter of the second threaded hole (502) is larger than the outer diameter of the first filter screen (7); One of the first end covers (8) is screwed onto each of the second threaded holes (502); One end of the first filter screen (7) is in contact with the side end of the electrophoresis tank (1), and the other end is in contact with the first end cover (8); One end of the condensation tube (4) is arranged on the inner side of the corresponding first filter screen (7).

5. A cryogel electrophoresis apparatus according to claim 4, characterized in that: Two condensing tubes (4) are provided, and the two condensing tubes (4) are mirror-symmetrical on both sides of the laminated frame (2), and one rotating drum (5) is correspondingly provided at both ends of each condensing tube (4).

6. A cryogel electrophoresis apparatus according to claim 5, characterized in that: It also includes a water inlet pipe (9) and a water outlet pipe (10), wherein: The water inlet pipe (9) and the water outlet pipe (10) are both three-way pipes; The two first water pipe joints (6) at one end of the two condensing pipes (4) are connected via the water inlet pipe (9), and the two first water pipe joints (6) at the other end are connected via the water outlet pipe (10).

7. A cryogel electrophoresis apparatus according to claim 6, characterized in that: The first water pipe joint (6) is detachably connected to the corresponding water inlet pipe (9) and the water outlet pipe (10).

8. A cryogel electrophoresis apparatus according to claim 7, characterized in that: It also includes a second water pipe joint (11), wherein: A second water pipe joint (11) is fixedly provided on one end of the water inlet pipe (9) and the water outlet pipe (10) away from the first water pipe joint (6).

9. A cryogel electrophoresis apparatus according to claim 8, characterized in that: The water inlet pipe (9) and the water outlet pipe (10) are both hoses.

10. A cryogel electrophoresis apparatus according to claim 3, characterized in that: It also includes a second filter screen (12) and a second end cover (13), wherein: The second filter screen (12) is coaxially arranged in one of the rotating drums (5); a third threaded hole (503) is arranged on the closed end of the rotating drum (5) on which the second filter screen (12) is arranged; the third threaded hole (503) and the corresponding rotating drum (5) are coaxially arranged; and the inner diameter of the third threaded hole (503) is greater than the outer diameter of the second filter screen (12); One end of the second end cover (13) is screwed into the third threaded hole (503); One end of the second filter screen (12) is in contact with the side end of the electrophoresis tank (1), and the other end is in contact with the second end cover (13); One end of the condenser tube (4) close to the second filter screen (12) is a water inlet end, and the other end is a water outlet end. The water inlet end of the condenser tube (4) is located on the inner side of the second filter screen (12).

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