Stem cell processing integrated device

Abstract

The utility model relates to the technical field of integrated devices, in particular to a stem cell processing integrated device. According to the technical scheme, the device comprises a box body and further comprises a sliding plate, the sliding plate is installed in the box body in a sliding mode, a supporting plate is fixedly installed on the box body, a containing plate is rotatably installed on the supporting plate, and an auxiliary device for automatically and evenly adding nutrient solution is arranged on the box body and comprises a liquid storage box fixedly installed at the top of the box body; a first air cylinder is fixedly installed on the sliding plate, a connecting block is fixedly installed at the bottom of the containing plate and hinged to a piston rod of the first air cylinder, and a second air cylinder is fixedly installed in the box body. According to the utility model, infection caused by contact between stem cells and air due to subsequent addition of a nutrient solution is avoided, the nutrient solution is uniformly added, bubbles are removed, the activity of the stem cells is improved, and the stability of a cell living environment is maintained.

Description

Technical Field

[0001] The utility model relates to the technical field of integrated devices, in particular to an integrated device for stem cell processing. Background Art

[0002] Stem cells are a type of cell that has unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated daughter cells. Stem cells have extremely broad application prospects in the fields of cell repair, developmental biology, and pharmacology in life sciences. Stem cells are often processed through processing devices for subsequent use.

[0003] When culturing stem cells, in order to maintain the normal life activities of the cells, it is usually necessary to add nutrient solution to provide the nutrients required by the cells and help the cells grow and reproduce. However, when preparing the nutrient solution, the nutrient solution needs to be stirred so that the nutrients in the nutrient solution can be fully mixed. However, stirring will cause more bubbles to exist in the nutrient solution. When stem cells come into contact with cells, larger bubbles will exert mechanical pressure on the cells, causing cell damage or death. Mechanical damage will seriously affect the survival and function of the cells. Therefore, the present application proposes an integrated device for stem cell processing. Summary of the invention

[0004] The utility model aims to provide an integrated device for stem cell processing in view of the problem that bubbles in the nutrient solution in the background technology cause stem cell damage.

[0005] The technical solution of the utility model: a stem cell processing integrated device, including a box body, and also includes:

[0006] A slide plate is slidably mounted inside a box body, a support plate is fixedly mounted on the box body, a placement plate is rotatably mounted on the support plate, and an auxiliary device for automatically and evenly adding nutrient solution is arranged on the box body.

[0007] Optionally, the auxiliary device includes a liquid storage tank fixedly mounted on the top of the box body, the first cylinder is fixedly mounted on the slide plate, the bottom of the placement plate is fixedly mounted with a connecting block, the connecting block is hinged to the piston rod of the first cylinder, the second cylinder is fixedly mounted in the box body, a support frame is fixedly mounted in the box body, the second cylinder is fixedly connected to the support frame, the piston rod of the second cylinder is fixedly mounted with a mounting plate, a guide cavity is slidably mounted on the bottom of the mounting plate, the guide cavity is a cavity setting, a dropper is fixedly mounted on the bottom of the guide cavity, the dropper is communicated with the guide cavity, vibration motors are fixedly mounted on both sides of the guide cavity, a connecting pipe is fixedly mounted on the bottom of the liquid storage tank, one end of the connecting pipe passes through the box body and is communicated with the guide cavity, a motor is fixedly mounted on one side of the box body, a reciprocating screw is fixedly mounted on the output shaft of the motor, a slide plate is slidably mounted in the box body, and the reciprocating screw is threadedly connected to the slide plate.

[0008] Optionally, a spring is fixedly mounted on the bottom of the mounting plate, and one end of the spring is fixedly connected to the guide cavity.

[0009] Optionally, a telescopic rod is fixedly mounted on the top of the slide plate, and one end of the telescopic rod is hinged to the placement plate.

[0010] Optionally, a guide column is fixedly installed in the box, and the slide plate is slidably connected to the guide column.

[0011] Optionally, a fixing frame is fixedly installed on the top of the box body, and the liquid storage tank is fixedly connected to the fixing frame.

[0012] Optionally, a mounting bracket is fixedly mounted on one side of the box body, and the motor is fixedly connected to the mounting bracket.

[0013] Optionally, a sealed door is hinged on one side of the box body, and a handrail is fixedly mounted on the sealed door.

[0014] Compared with the prior art, the utility model has the following beneficial technical effects:

[0015] 1. By starting to open the valve in the liquid storage tank, the nutrient solution in the liquid storage tank is transported from the connecting pipe to the diversion chamber, and the second cylinder is started. The piston rod of the second cylinder drives the diversion chamber to move downward, and the nutrient solution transported to the diversion chamber drips from the dropper into the container, thereby preventing the stem cells from being infected by contact with the air due to the subsequent addition of nutrient solution, and maintaining the stability of the cell living environment;

[0016] 2. By starting the first cylinder, the piston rod of the first cylinder drives the placement plate to slowly shake in the vertical direction, and cooperates with the starting motor and the vibration motor. The output shaft of the motor drives the reciprocating screw to rotate, so that the placement plate performs horizontal reciprocating motion in the horizontal direction. The vibration motor drives the dropper at the bottom of the diversion cavity to vibrate, and the bubbles in the nutrient solution in the dropper are vibrated upward to avoid the bubbles adhering to the nutrient solution and being transported to the stem cell container, causing uneven contact between the nutrient solution and the stem cells, thereby achieving uniform addition of the nutrient solution, promoting the growth of stem cells, avoiding loss of stem cells, and improving the activity of stem cells.

[0017] The utility model can avoid infection caused by contact of stem cells with air due to subsequent addition of nutrient solution, evenly add nutrient solution and remove air bubbles, thereby improving the activity of stem cells and maintaining the stability of the cell living environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The internal structure of an embodiment of the utility model is shown as follows: Figure 1 ;

[0019] Figure 2 A structural schematic diagram of an embodiment of the utility model is given;

[0020] Figure 3 The internal structure of an embodiment of the utility model is shown as follows: Figure 2 ;

[0021] Figure 4 A schematic diagram of the second cylinder, the flow guide cavity and the dropper structure of an embodiment of the utility model is given;

[0022] Figure 5 for Figure 3 Schematic diagram of the local enlarged structure at point A in the middle.

[0023] Figure numerals: 1. Box body; 2. Sealed door; 3. Handrail; 4. Motor; 5. Mounting frame; 6. Liquid storage tank; 7. Fixed frame; 8. Placement plate; 9. First cylinder; 10. Guide column; 11. Slide plate; 12. Telescopic rod; 13. Support plate; 14. Reciprocating screw rod; 15. Guide chamber; 16. Second cylinder; 17. Support frame; 18. Mounting plate; 19. Connecting block; 20. Spring; 21. Vibration motor; 22. Dropper; 23. Connecting pipe. DETAILED DESCRIPTION

[0024] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and specific embodiments. Example

[0025] like Figure 1-2As shown, the utility model proposes an integrated device for stem cell processing, including a box body 1, and also includes a slide plate 11, the slide plate 11 is slidably installed inside the box body 1, a support plate 13 is fixedly installed on the box body 1, and a placement plate 8 is rotatably installed on the support plate 13 for placing a culture container containing stem cells, and an auxiliary device for automatically and evenly adding nutrient solution is provided on the box body 1 to avoid contact with air when adding nutrient solution to the stem cells, causing stem cell contamination.

[0026] like Figure 3-4 As shown, the present embodiment also includes an auxiliary device, which includes a liquid storage tank 6 fixedly mounted on the top of the box body 1, a valve is arranged in the liquid storage tank 6 for storing nutrient solution, a first cylinder 9 is fixedly mounted on the slide plate 11, a connecting block 19 is fixedly mounted on the bottom of the placement plate 8, the connecting block 19 is hinged to the piston rod of the first cylinder 9, a second cylinder 16 is fixedly mounted in the box body 1, a support frame 17 is fixedly mounted in the box body 1, the second cylinder 16 is fixedly connected to the support frame 17, and the second cylinder 16 is fixedly mounted on the support frame 17. The piston rod is fixedly installed with a mounting plate 18, and a guide chamber 15 is slidably installed at the bottom of the mounting plate 18. The guide chamber 15 is a cavity setting. A dropper 22 is fixedly installed at the bottom of the guide chamber 15, and the dropper 22 is connected to the guide chamber 15. Vibration motors 21 are fixedly installed on both sides of the guide chamber 15. A connecting pipe 23 is fixedly installed at the bottom of the liquid storage tank 6. One end of the connecting pipe 23 passes through the box body 1 and is connected to the guide chamber 15. A motor 4 is fixedly installed on one side of the box body 1, and a reciprocating motor 21 is fixedly installed on the output shaft of the motor 4. The box 1 is provided with a slide plate 11 which is slidably mounted in the screw rod 14. The reciprocating screw rod 14 is threadedly connected to the slide plate 11. When the nutrient solution needs to be added to the stem cells, the motor 4 is started. The output shaft of the motor 4 drives the reciprocating screw rod 14 to rotate. Since the slide plate 11 is threadedly connected to the reciprocating screw rod 14, when the reciprocating screw rod 14 rotates, the slide plate 11 performs horizontal reciprocating motion on the reciprocating screw rod 14. The first cylinder 9 and the second cylinder 16 are started. The piston rod of the first cylinder 9 drives the placement plate 8 to slowly shake up and down. The second cylinder 16 is driven by the piston rod of the first cylinder 9. The piston rod of the cylinder 16 drives the guide chamber 15 to descend. When the dropper 22 approaches the surface of the stem cell container, the valve of the liquid storage tank 6 is opened, and the nutrient solution is transported to the dropper through the connecting pipe. The vibration motor 21 is started, and the vibration motor 21 drives the guide chamber 15 to vibrate, so that the bubbles in the nutrient solution in the dropper 22 are shaken upward to prevent the bubbles from dripping from the nutrient solution into the culture container. In combination with the horizontal reciprocating motion and slow up and down shaking of the placement plate 8, the nutrient solution in the dropper 22 can evenly cover the cell surface.

[0027] like Figure 3-5 As shown, a spring 20 is fixedly installed at the bottom of the mounting plate 18, one end of the spring 20 is fixedly connected to the guide cavity 15 to increase the vibration effect, a telescopic rod 12 is fixedly installed on the top of the slide plate 11, one end of the telescopic rod 12 is hinged to the placement plate 8, a guide column 10 is fixedly installed in the box body 1, and the slide plate 11 is slidably connected to the guide column 10.

[0028] like Figure 1-2 As shown, a fixing frame 7 is fixedly installed on the top of the box body 1, and the liquid storage tank 6 is fixedly connected to the fixing frame 7. A mounting frame 5 is fixedly installed on one side of the box body 1, and the motor 4 is fixedly connected to the mounting frame 5. A sealed door 2 is hinged on one side of the box body 1, and a handrail 3 is fixedly installed on the sealed door 2.

[0029] Working principle: when it is necessary to add nutrient solution to the container on the placement plate 8, the motor 4 is started, and the output shaft of the motor 4 drives the reciprocating screw 14 to rotate. Since the slide plate 11 is threadedly connected to the reciprocating screw 14, when the reciprocating screw 14 rotates, the slide plate 11 performs horizontal reciprocating motion on the reciprocating screw 14, and the first cylinder 9 and the second cylinder 16 are started. The piston rod of the first cylinder 9 drives the placement plate 8 to shake up and down slowly, and the piston rod of the second cylinder 16 drives the guide chamber 15 to descend. When the dropper 22 is close to the surface of the stem cell container, the valve of the liquid storage tank 6 is opened. The nutrient solution is transported to the dropper through the connecting tube, and the vibration motor 21 is started. The vibration motor 21 drives the guide chamber 15 to vibrate, and the bubbles in the nutrient solution in the dropper 22 are shaken upward to prevent the bubbles from dripping from the nutrient solution into the culture container. With the horizontal reciprocating motion and slow up and down shaking of the placement plate 8, the nutrient solution in the dropper 22 can be evenly covered on the cell surface, so that the subsequent addition of nutrient solution can be avoided. The stem cells are prevented from being infected by contact with the air, the nutrient solution is evenly added and the bubbles are removed, the activity of the stem cells is improved, and the stability of the cell living environment is maintained.

[0030] The above-mentioned specific embodiments are only several optional embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A stem cell processing integrated device, comprising a housing (1), characterized in that: Also includes: A slide plate (11) is slidably mounted inside a box body (1); a support plate (13) is fixedly mounted on the box body (1); a placement plate (8) is rotatably mounted on the support plate (13); and an auxiliary device for automatically and evenly adding nutrient solution is provided on the box body (1).

2. The stem cell processing integrated device according to claim 1, characterized in that: The auxiliary device comprises a liquid storage tank (6) fixedly mounted on the top of the box body (1); a first cylinder (9) is fixedly mounted on the slide plate (11); a connecting block (19) is fixedly mounted on the bottom of the placement plate (8); the connecting block (19) is hingedly connected to the piston rod of the first cylinder (9); a second cylinder (16) is fixedly mounted in the box body (1); a support frame (17) is fixedly mounted in the box body (1); the second cylinder (16) is fixedly connected to the support frame (17); a mounting plate (18) is fixedly mounted on the piston rod of the second cylinder (16); a guide chamber (15) is slidably mounted on the bottom of the mounting plate (18); the guide chamber (15) is a cavity arrangement, a dropper (22) is fixedly installed at the bottom of the guide cavity (15), the dropper (22) is communicated with the guide cavity (15), vibration motors (21) are fixedly installed on both sides of the guide cavity (15), a connecting pipe (23) is fixedly installed at the bottom of the liquid storage tank (6), one end of the connecting pipe (23) passes through the box body (1) and is communicated with the guide cavity (15), a motor (4) is fixedly installed on one side of the box body (1), a reciprocating screw rod (14) is fixedly installed on the output shaft of the motor (4), a slide plate (11) is slidably installed in the box body (1), and the reciprocating screw rod (14) is threadedly connected to the slide plate (11).

3. The stem cell processing integrated device according to claim 2, characterized in that: A spring (20) is fixedly mounted on the bottom of the mounting plate (18), and one end of the spring (20) is fixedly connected to the flow guide cavity (15).

4. The stem cell processing integrated device according to claim 2, characterized in that: A telescopic rod (12) is fixedly mounted on the top of the slide plate (11), and one end of the telescopic rod (12) is hinged to the placement plate (8).

5. The stem cell processing integrated device according to claim 2, characterized in that: A guide column (10) is fixedly installed in the box body (1), and the slide plate (11) is slidably connected to the guide column (10).

6. The integrated stem cell processing device according to claim 2, characterized in that: A fixing frame (7) is fixedly mounted on the top of the box body (1), and the liquid storage box (6) is fixedly connected to the fixing frame (7).

7. The integrated stem cell processing device according to claim 2, characterized in that: A mounting frame (5) is fixedly mounted on one side of the box body (1), and the motor (4) is fixedly connected to the mounting frame (5).

8. The stem cell processing integrated device according to claim 1, characterized in that: A sealed door (2) is hingedly connected to one side of the box body (1), and a handrail (3) is fixedly mounted on the sealed door (2).

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