Mouse bone marrow cell extraction device

Abstract

The utility model relates to the technical field of bone marrow cell extraction, and particularly discloses a mouse bone marrow cell extraction device which comprises a centrifugal device for cell extraction and a centrifugal test tube matched with the centrifugal device, the centrifugal device comprises a centrifugal cylinder and a bottom plate, the inner side of the centrifugal cylinder is matched with the centrifugal test tube, a gear ring is arranged on the outer side of the centrifugal cylinder, a transmission gear is mounted on the outer side of the gear ring, and a driving part is arranged below the transmission gear; according to the utility model, the centrifugal test tube is matched with the combination of the temporary storage cavity and the filter screen which are arranged in the body and the combination of the conveying pipe and the pipeline, a target object in the temporary storage cavity is flushed by culture medium liquid through the pump body, bone marrow cells are fully released, and bone residues are prevented from falling into the solution through filtration of the filter screen; in addition, in the washing process, centrifugation is matched, so that the purification efficiency and concentration of the bone marrow cells can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of bone marrow cell extraction, in particular to a mouse bone marrow cell extraction device. Background Art

[0002] One of the steps in the mouse bone marrow cell isolation process is to inject culture medium into the bone marrow cavity of long bones using a syringe and a sterile needle, then gently shake the long bones to help release bone marrow cells from the bone marrow cavity, collect the released bone marrow cells into a sterile centrifuge tube or culture dish using a sterile needle or pipette, and centrifuge the collected bone marrow cells to remove the culture medium and cell debris.

[0003] When using a syringe and a needle for aspiration, sometimes the bone marrow cells are not released thoroughly enough, resulting in insufficient concentration in the later purification. In a Chinese patent: A mouse bone marrow cell extraction device (publication number: CN214437150U), the design of the filter mechanism can filter the residue while centrifuging. When in use, cut the two ends of the mouse long bone and place it in a centrifuge container, then place the centrifuge container in a centrifuge collection tube and cover it with a sealing cover, place it in a centrifuge for centrifugation, and after completion, take out the centrifuge container and the filter mechanism, and obtain the target bone marrow cells under the centrifuge collection tube.

[0004] However, due to the small volume of the test tube, the solution cannot be fully immersed, resulting in incomplete cell release. Based on this, it is necessary to improve it. Utility Model Content

[0005] In view of the deficiencies of the prior art, the utility model provides a mouse bone marrow cell extraction device, which solves the problem in the prior art that due to the small volume of the test tube, the solution cannot be fully immersed, resulting in insufficient cell release.

[0006] The mouse bone marrow cell extraction device of the utility model comprises a centrifugal device for cell extraction and a centrifugal test tube adapted to the centrifugal device;

[0007] The centrifugal device comprises a centrifugal cylinder and a bottom plate, the inner side of the centrifugal cylinder is adapted to the centrifugal test tube, the outer side of the centrifugal cylinder is provided with a gear ring, the outer side of the gear ring is installed with a transmission gear, a driving member is provided below the transmission gear, and the driving member drives the transmission gear to drive the centrifugal cylinder to rotate on the bottom plate;

[0008] The top cover of the centrifugal test tube is provided with a cartridge for fixing the centrifugal test tube;

[0009] The centrifuge test tube comprises a body, a sealing plug is arranged on the top of the body, a through hole is provided in the middle of the sealing plug, and a delivery pipe is installed, a through hole is provided on the top of the sealing plug, and a pipeline is plugged into the top of the through hole;

[0010] A temporary storage cavity is arranged at the bottom of the sealing plug, the inner side of the temporary storage cavity is filled with a target object, and a filter is arranged at the bottom of the temporary storage cavity for filtering the cell fluid.

[0011] As a further improvement of the present invention, one or more ribs are arranged in an annular array on the outer side of the sealing plug to increase the friction force on the outer side of the sealing plug.

[0012] As a further improvement of the present invention, the sealing plug is configured in a "conical" shape, and the bottom of the sealing plug extends downward. A fixed distance is maintained between the extended area of ​​the sealing plug and the body, and they are adapted through a slip ring.

[0013] As a further improvement of the present invention, two mirror-symmetrical grooves are arranged at the ribs on the outside of the sealing plug, and the grooves are adapted to the cartridge to fix the sealing plug.

[0014] As a further improvement of the present invention, one side of the cartridge is provided with one or more extension rods, and one end of the extension rod is provided with a support column for fixing the cartridge.

[0015] As a further improvement of the present invention, one end of the delivery pipe is connected to a pipeline, and the pipeline extends to the inner side of the through hole and seals it.

[0016] As a further improvement of the present invention, the pipe body of the pipeline passes through the top of the cartridge and is connected to an external pump body, so as to flush the target object inside the temporary storage chamber.

[0017] As a further improvement of the present invention, a limiting groove is provided on the outer side of the body near the bottom, and the limiting groove is adapted to the clamping block inside the centrifugal cylinder.

[0018] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0019] The utility model uses a centrifugal test tube, a temporary storage chamber and a filter screen arranged in the body, and a delivery tube and a pipeline to flush the target object in the temporary storage chamber with culture medium liquid through a pump body, so that the bone marrow cells are fully released and filtered through the filter screen to prevent bone residues from falling into the solution. In addition, during the flushing process, centrifugation is used to improve the purification efficiency and concentration of the bone marrow cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0021] Figure 1 This is a schematic diagram of the combined structure of the centrifugal device and the centrifugal test tube of the utility model;

[0022] Figure 2 This is a schematic diagram of the three-dimensional structure of the centrifugal test tube of the utility model;

[0023] Figure 3 This is a schematic diagram of the front view structure of the centrifugal test tube of the utility model;

[0024] Figure 4 This is a schematic diagram of the top view of the centrifugal test tube of the utility model;

[0025] Figure 5 This is a schematic diagram of the front view structure of the centrifugal test tube of the utility model;

[0026] Figure 6 For this utility model Figure 5 Schematic diagram of the AA section structure;

[0027] Figure 7 For this utility model Figure 6 Enlarged structural diagram at A in the middle.

[0028] In the figure: 1. centrifugal device; 2. centrifugal test tube;

[0029] 11. Support column; 12. Transmission gear; 13. Driving member; 14. Centrifugal cylinder; 15. Cartridge; 16. Pipeline;

[0030] 21. Limiting groove; 22. Main body; 23. Sealing plug; 24. Through hole; 25. Ribbon; 26. Slip ring; 27. Delivery pipe; 28. Filter screen; 29. ​​Temporary storage chamber. DETAILED DESCRIPTION

[0031] The following will disclose multiple embodiments of the utility model with diagrams. For the purpose of clear description, many physical details will be described together in the following description. However, it should be understood that these physical details should not be used to limit the utility model. In other words, in some embodiments of the utility model, these physical details are not necessary. In addition, for the purpose of simplifying the diagram, some conventional structures and components will be depicted in a simple schematic manner in the diagram.

[0032] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0033] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 as well as Figure 6 During the separation of mouse bone marrow cells, due to the small volume of the test tube, the solution cannot be fully immersed, resulting in incomplete cell release. Based on this, the present application provides a mouse bone marrow cell extraction device, including a centrifugal device 1 for cell extraction and a centrifugal test tube 2 adapted to the centrifugal device 1;

[0034] The centrifugal device 1 includes a centrifugal cylinder 14 and a bottom plate. The inner side of the centrifugal cylinder 14 is adapted to the centrifugal test tube 2. A gear ring is provided on the outer side of the centrifugal cylinder 14. A transmission gear 12 is installed on the outer side of the gear ring. A driving member 13 is provided below the transmission gear 12. The driving member 13 drives the transmission gear 12 to drive the centrifugal cylinder 14 to rotate on the bottom plate.

[0035] The top cover of the centrifugal test tube 2 is provided with a cartridge 15 for fixing the centrifugal test tube 2;

[0036] The centrifugal test tube 2 includes a body 22, a sealing plug 23 is provided on the top of the body 22, a through hole is provided in the middle of the sealing plug 23, and a delivery pipe 27 is installed, a through hole 24 is provided on the top of the sealing plug 23, and a pipe 16 is inserted into the top of the through hole 24;

[0037] A temporary storage cavity 29 is provided at the bottom of the sealing plug 23. The inside of the temporary storage cavity 29 is filled with the target object. A filter screen 28 is provided at the bottom of the temporary storage cavity 29 for filtering the cell fluid.

[0038] The centrifugal device 1 is composed of a centrifugal cylinder 14 and a bottom plate. The inner side of the centrifugal cylinder 14 is adapted to the centrifugal test tube 2 to ensure that the test tube is firmly fixed in the centrifugal device 1. A gear ring is provided on the outer side of the centrifugal cylinder 14, and a transmission gear 12 is installed on the outer side of the gear ring. These components cooperate with each other to ensure that the centrifugal test tube 2 can rotate stably during the centrifugal operation. A driving member 13 is provided below the transmission gear 12. The driving member 13 drives the transmission gear 12, thereby driving the centrifugal cylinder 14 to rotate on the bottom plate, so that the centrifugal operation can be carried out smoothly.

[0039] A cartridge 15 is provided on the top of the centrifugal test tube 2 for fixing the centrifugal test tube 2 to ensure that the test tube is in a stable position during the centrifugal operation and will not move or tilt.

[0040] A sealing plug 23 is provided at the top of the centrifugal test tube 2 body 22, a through hole is provided in the middle of the sealing plug 23, and a delivery tube 27 is installed. A through hole 24 is provided at the top of the sealing plug 23, and a pipe 16 is plugged into the top of the through hole 24. These arrangements allow the culture medium liquid to be smoothly delivered to the centrifugal test tube 2 through the delivery pipe 27 and the pipe 16.

[0041] A temporary storage chamber 29 is provided at the bottom of the sealing plug 23. The inside of the temporary storage chamber 29 is filled with the target object, i.e., the mouse bone marrow cells to be extracted. A filter 28 is provided at the bottom of the temporary storage chamber 29 for filtering the cell fluid to ensure that impurities and bone residues are removed during the extraction process.

[0042] A temporary storage chamber 29 and a filter screen 28 are provided in the centrifugal test tube 2, so that the bone marrow cells can be fully released during the centrifugal operation, and impurities can be effectively filtered during the collection process, thereby improving the efficiency of cell extraction. The filter screen 28 is used to effectively remove impurities such as bone residues, ensure the purity of the extracted sample, and facilitate subsequent experiments. The centrifugal test tube 2 is fixed by the top cover and the cartridge 15, and the transmission parts of the centrifugal device 1 cooperate with each other, so that the test tube is stable during operation, the possibility of operating errors is reduced, and the stability and reliability of the operation are improved.

[0043] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 as well as Figure 7 The outer side of the sealing plug 23 is provided with one or more ribs 25 in an annular array for increasing the friction force on the outer side of the sealing plug 23 .

[0044] The sealing plug 23 is configured in a “conical” shape, and the bottom of the sealing plug 23 extends downward. A fixed distance is maintained between the extended area of ​​the sealing plug 23 and the body 22 , and they are adapted through a slip ring 26 .

[0045] Two mirror-symmetrical grooves are arranged at the ribs 25 on the outside of the sealing plug 23 , and the grooves are adapted to the cartridge 15 for fixing the sealing plug 23 .

[0046] The effect of the ribs 25 is to increase the friction force on the outside of the sealing plug 23 to ensure the stability and sealing performance of the sealing plug 23 in the test tube. By increasing the friction force, the sealing plug 23 can be effectively prevented from accidentally moving or sliding during operation.

[0047] The sealing plug 23 is set in a "conical" shape, which is conducive to better fitting the inner wall of the test tube when sealing the test tube, ensuring a good sealing effect. At the same time, the bottom of the sealing plug 23 extends downward and maintains a fixed distance with the body 22. This extended area is adapted by the sliding ring 26 to ensure that the sealing plug 23 is firmly fixed in the test tube and is not easy to loosen or shift.

[0048] Two mirror-symmetrical grooves are provided at the ribs 25 on the outside of the sealing plug 23, which are adapted to the cartridge 15 on the top cover of the test tube to more firmly fix the sealing plug 23. Through this design, the sealing plug 23 can be rotatably connected to the test tube without affecting the rotation of the test tube body 22, and is not easily moved or fallen off due to external forces.

[0049] By arranging the ribs 25 on the outside of the sealing plug 23, the friction force on the outside of the sealing plug 23 is increased, and the stability of the sealing plug 23 in the test tube is improved. The sealing plug 23 is arranged in a "conical" shape, which fits better with the inner wall of the test tube, ensuring a good sealing effect and preventing leakage of the solution. Through the cooperation of the card slot and the cartridge 15, the sealing plug 23 is effectively fixed in the test tube, and is not easily loosened or shifted due to external interference, thereby improving the stability and reliability of the operation.

[0050] See also Figure 1 , Figure 2 as well as Figure 7 One side of the cartridge 15 is provided with one or more extension rods, and one end of the extension rod is provided with a support column 11 for fixing the cartridge 15 .

[0051] One end of the delivery pipe 27 is connected to the pipe 16 , which extends to the inside of the through hole 24 and seals it.

[0052] The pipe body of the pipeline 16 passes through the top of the cartridge 15 and is connected to an external pump body, so as to flush the target object inside the temporary storage chamber 29 .

[0053] A limiting groove 21 is provided on the outer side of the body 22 near the bottom, and the limiting groove 21 is adapted to the inner block of the centrifugal cylinder 14 .

[0054] The cartridge 15 is fixed by the support column 11 to ensure the stability and position of the cartridge 15 during operation, so that the cartridge 15 fits with the sealing plug 23 on the centrifuge tube 2 and will not shift or loosen due to external vibration or pressure.

[0055] One end of the delivery tube 27 is connected to the pipe 16, which extends to the inner side of the through hole 24 and is sealed to ensure that the liquid will not leak or overflow during the delivery process, thereby ensuring that the culture medium liquid can be smoothly delivered to the centrifuge tube 2 without affecting the stability and reliability of the experimental process.

[0056] The pipe body of the pipe 16 passes through the top of the cartridge 15 and is connected to the external pump body, and is used to flush the target (bone marrow cells) inside the temporary storage chamber 29. Flushing the target in the temporary storage chamber 29 by the pump body can ensure that the bone marrow cells can be fully released, and in the flushing process, it cooperates with the filter 28 to filter impurities, thereby improving the purity and concentration of the extracted sample.

[0057] A limiting groove 21 is provided on the outer side of the main body 22 near the bottom, and the limiting groove 21 is adapted to the inner block of the centrifuge cylinder 14 to limit the position and movement range of the centrifuge tube 2 during the centrifugal operation. Such a design ensures that the centrifuge tube 2 is stably fixed in the centrifuge device 1 during the operation, and will not produce unnecessary movement or swing due to the action of centrifugal force.

[0058] The cooperation between the extension rod and the support column 11, the limit groove 21 and the card block ensures the stability of each component during operation, reduces the possibility of accidental movement or loosening, and improves the stability of operation. The setting of the delivery tube 27 and the pipeline 16 and the sealing design of the through hole 24 ensure that the culture medium liquid is smoothly delivered to the test tube, while preventing liquid leakage, ensuring the smooth progress of the experimental process. The target object in the temporary storage chamber 29 is flushed by the pump body, and the filter screen 28 is used to filter impurities, thereby improving the efficiency of cell extraction and the purity of the sample, which is beneficial to the accuracy and reliability of subsequent experiments.

[0059] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the scope of the claims of the present invention.

Claims

1. A mouse bone marrow cell extraction device, comprising a centrifugal device (1) for cell extraction and a centrifugal test tube (2) adapted to the centrifugal device (1); Features: The centrifugal device (1) comprises a centrifugal cylinder (14) and a bottom plate, the inner side of the centrifugal cylinder (14) is adapted to the centrifugal test tube (2), the outer side of the centrifugal cylinder (14) is provided with a gear ring, the outer side of the gear ring is provided with a transmission gear (12), a driving member (13) is provided below the transmission gear (12), and the driving member (13) drives the transmission gear (12) to drive the centrifugal cylinder (14) to rotate on the bottom plate; The top cover of the centrifugal test tube (2) is provided with a cartridge (15) for fixing the centrifugal test tube (2); The centrifugal test tube (2) comprises a body (22), a sealing plug (23) is arranged on the top of the body (22), a through hole is provided in the middle of the sealing plug (23), and a conveying pipe (27) is installed, a through hole (24) is provided on the top of the sealing plug (23), and a pipeline (16) is inserted into the top of the through hole (24); A temporary storage cavity (29) is provided at the bottom of the sealing plug (23), the inner side of the temporary storage cavity (29) is filled with a target object, and a filter screen (28) is provided at the bottom of the temporary storage cavity (29) for filtering the cell fluid.

2. A mouse bone marrow cell extraction device according to claim 1, characterized in that: One or more ribs (25) are arranged in an annular array on the outside of the sealing plug (23) to increase the friction force on the outside of the sealing plug (23).

3. A mouse bone marrow cell extraction device according to claim 1, characterized in that: The sealing plug (23) is arranged in a "conical" shape, and the bottom of the sealing plug (23) extends downward. A fixed distance is maintained between the extended area of ​​the sealing plug (23) and the body (22), and they are adapted through a slip ring (26).

4. The mouse bone marrow cell extraction device according to claim 1, characterized in that: Two mirror-symmetrical clamping grooves are arranged at the ribs (25) on the outside of the sealing plug (23), and the clamping grooves are adapted to the clamping cylinder (15) and are used to fix the sealing plug (23).

5. The mouse bone marrow cell extraction device according to claim 1, characterized in that: One side of the cartridge (15) is provided with one or more extension rods, and one end of the extension rod is provided with a support column (11) for fixing the cartridge (15).

6. A mouse bone marrow cell extraction device according to claim 1, characterized in that: One end of the delivery pipe (27) is connected to a pipe (16), and the pipe (16) extends to the inside of the through hole (24) and is sealed thereto.

7. A mouse bone marrow cell extraction device according to claim 6, characterized in that: The pipe body of the pipeline (16) passes through the top of the cartridge (15) and is connected to an external pump body, and is used to flush the target object inside the temporary storage chamber (29).

8. The mouse bone marrow cell extraction device according to claim 1, characterized in that: A limiting groove (21) is provided on the outer side of the body (22) near the bottom, and the limiting groove (21) is adapted to fit with a clamping block inside the centrifugal cylinder (14).

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