Sample detection card box

Abstract

The utility model relates to the technical field of sample detection, and discloses a sample detection card box which comprises a base and a sample adding cover, and a plurality of storage bins are arranged on the base. The sample adding cover covers the base in a sealing mode, a movement clamping groove used for being connected with the sample mixing device in a clamping mode is formed in the sample adding cover, the sample adding cover is used for rotating around the central axis of the sample adding cover relative to the base under driving of the sample mixing device, a solution in the sample adding cover can be sequentially mixed with sample treatment objects in the containing bin, and the sample adding cover is simple in structure. The sample adding cover is provided with a sample adding hole used for being communicated with the outside, and the sample adding hole is further used for being in butt joint with the containing bin in the rotating process. The sample adding cover is driven by the sample mixing device to rotate and adjust, so that the sample adding hole can be aligned with the storage bin more accurately, the butt joint precision of the sample adding hole and the storage bin is improved, and efficient injection of a reagent is ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of sample detection, and in particular relates to a sample detection card box. Background Art

[0002] PCR technology is a molecular biology technology that amplifies specific deoxyribonucleic acid sequences in vitro. PCR technology is widely used in nucleic acid detection and analysis due to its strong specificity, high sensitivity, low purity requirements, simplicity and rapidity. In order to achieve instant nucleic acid detection and improve detection accuracy, it is often necessary to pre-set various reagents in the test kit, and to extract and purify nucleic acids through multiple additions and pipetting of mixed reagents. The nucleic acid detection process involves a variety of solutions and reagents, and there are many pipetting steps, which is relatively complicated. In addition, the sample processing is always in the external environment, and the sample processing process is easily contaminated by the external environment. Utility Model Content

[0003] The utility model aims to provide a sample detection card box to solve the technical problems that the sample is inconvenient to handle and easy to be contaminated.

[0004] In order to achieve the above object, the utility model provides a sample detection card box, the sample detection card box comprising:

[0005] The base is provided with a plurality of storage compartments;

[0006] The sample adding cover and the sealing cover are closed on the base. The sample adding cover is provided with a moving slot for engaging with the sample mixing device. The sample adding cover is used to rotate relative to the base under the drive of the sample mixing device. The sample adding cover is provided with a sample adding hole for communicating with the outside world. Multiple storage bins are used to communicate with the sample adding cover.

[0007] In an embodiment of the utility model, the motion slot is disposed on the top of the sample loading cover and penetrates the sample loading cover in a radial direction of the sample loading cover, and an opening is disposed on a side of the motion slot away from the base.

[0008] In an embodiment of the present utility model, the motion card slot includes:

[0009] An insertion section, wherein the opening is arranged at the top end of the insertion section;

[0010] The limiting section is connected to the bottom end of the inserting section, the cross-sectional dimension of the inserting section is smaller than the cross-sectional dimension of the limiting section, and the bottom surface of the inserting section is used to abut against the limiting sample mixing device.

[0011] In an embodiment of the utility model, guide slopes are provided at both ends of the groove side walls of the insertion section and the limiting section along the length direction. The guide slopes are inclined from the outer side of the sample loading cover toward the groove side walls. The guide slopes are used to guide the sample mixing device into the motion card slot.

[0012] In an embodiment of the utility model, a magnetic attraction component for absorbing magnetic beads is provided on the sample adding cover.

[0013] In an embodiment of the utility model, a magnetic hole is provided on one side of the sample adding cover facing the base, and the magnetic attraction member is sealed in the magnetic hole.

[0014] In an embodiment of the utility model, a pipetting hole is further provided on one side of the sample loading cover facing the base, and one end of the pipetting hole is open and used for receiving the storage bin.

[0015] In an embodiment of the present invention, the pipetting hole is arranged close to the sample adding hole.

[0016] In an embodiment of the present utility model, the centers of the pipetting hole, the sample adding hole and the magnetic suction hole are located on an arc with the same radius.

[0017] In an embodiment of the utility model, the motion slot is arranged in the middle of the sample loading cover and divides the sample loading cover into two top cover parts of the same shape, and the pipetting hole, the sample loading hole and the magnetic suction hole are all arranged on the top cover part on either side of the motion slot.

[0018] Through the above technical solution, the sample detection cartridge provided by the embodiment of the utility model has the following beneficial effects:

[0019] The sample detection card box in this embodiment includes a base and a sample loading cover. A plurality of storage bins are provided on the base, and the sample loading cover sealing cover is closed on the base and provided with a motion card slot for engaging with the sample mixing device. The sample loading cover is driven by the sample mixing device to rotate around its own central axis so that the sample loading cover rotates relative to the base, so that the solution in the sample loading cover can be mixed with the sample processing material in the storage bin in sequence, and the sample loading cover has a simple structure. In addition, in the process of the sample mixing device driving the sample loading cover to rotate and adjust, the sample loading hole can be more accurately aligned with the storage bin, the docking accuracy of the sample loading hole and the storage bin is improved, and the efficient injection of the reagent is ensured. The sample processing material in the base and the liquid in the sample loading cover can be mixed in sequence only by rotating the sample loading cover, without the participation of an external pipetting tool, which can simplify the sample processing steps, facilitate sample processing, and ensure that the sample processing process is always in a closed sample detection card box, will not be exposed to the external environment, and can avoid the situation of being contaminated by the external environment during the sample processing process.

[0020] Other features and advantages of the embodiments of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings are used to provide a further understanding of the embodiments of the present utility model and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present utility model, but do not constitute a limitation on the embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without creative work. In the drawings:

[0022] Figure 1 It is a schematic diagram of the structure of the sample detection cartridge according to the utility model;

[0023] Figure 2 It is a structural schematic diagram of the sample adding cover according to the utility model at one viewing angle;

[0024] Figure 3 It is a structural schematic diagram of the sample adding cover according to the utility model from another viewing angle.

[0025] Description of Reference Numerals

[0026] 1 Base 23 Magnetic hole

[0027] 2 Sample cover 24 pipetting holes

[0028] 21 Sports card slot 3 hole cover

[0029] 211 Insertion segment 4 Buckle

[0030] 212 Limiting section 5 Rotating protrusion

[0031] 213 Guide slope 6 Limiting protrusion

[0032] 22 sample wells DETAILED DESCRIPTION

[0033] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0034] The sample detection cartridge according to the present utility model is described below with reference to the accompanying drawings.

[0035] like Figure 1 As shown, in this embodiment, a sample detection card box is proposed, and the sample detection card box includes a base 1 and a sample loading cover 2. A plurality of storage bins are arranged on the base 1. The sample loading cover 2 is sealed and covered on the base 1, and the sample loading cover 2 is provided with a motion card slot 21 for engaging with a sample mixing device. The sample loading cover 2 is used to rotate around its own central axis relative to the base 1 under the drive of the sample mixing device. The sample loading cover 2 is provided with a sample loading hole 22 for communicating with the outside world, and the plurality of storage bins are used to be connected with the sample loading cover 2 in sequence.

[0036] It should be noted that the sample detection card box in this embodiment can be used for nucleic acid detection, such as a nucleic acid extraction card box for magnetic bead extraction of nucleic acid. The multiple storage compartments can be storage spaces for different reaction solutions or enzymes. The sample mixing device can adopt the rotation drive mechanism and flip drive mechanism in the prior art, which can drive the sample loading cover 2 to rotate relative to the base 1, and can also drive the entire sample detection card box to flip back and forth in the vertical direction.

[0037] The sample detection cartridge in this embodiment includes a base 1 and a sample loading cover 2. A plurality of storage bins are provided on the base 1, and the sample loading cover 2 is sealed and covered on the base 1 and provided with a motion slot 21 for engaging with a sample mixing device. The sample loading cover 2 is driven by the sample mixing device to rotate around its own central axis so that the sample loading cover 2 rotates relative to the base 1, so that the solution in the sample loading cover 2 can be mixed with the sample processing material in the storage bin in sequence, and the sample loading cover 2 has a simple structure. In addition, in the process of the sample mixing device driving the sample loading cover 2 to rotate and adjust, the sample loading hole 22 can be more accurately aligned with the storage bin, which improves the docking accuracy of the sample loading hole 22 and the storage bin and ensures efficient injection of the reagent. The sample processing material in the base 1 and the liquid in the sample loading cover 2 can be mixed in sequence only by rotating the sample loading cover, without the participation of an external pipetting tool, which can simplify the sample processing steps, facilitate sample processing, and ensure that the sample is always in a closed sample detection cartridge during the sample processing process, and will not be exposed to the external environment, which can avoid the situation of being contaminated by the external environment during the sample processing process. Among them, under the drive of the sample mixing device, the sample adding cover 2 and the base 1 can be flipped together and switched between a normal state and a flipped state. In the normal state, the sample adding cover 2 is located above the base 1, and in the flipped state, the sample adding cover 2 is located below the base 1, which improves the coordination effect between the hole position on the sample adding cover 2 and the storage bin on the base 1, and facilitates the close docking of the hole position and the storage bin.

[0038] like Figure 1 and Figure 2As shown, in this embodiment, the motion card slot 21 is opened at the top of the sample loading cover 2 and the motion card slot 21 penetrates the sample loading cover 2 in the radial direction of the sample loading cover 2. The side of the motion card slot 21 away from the base 1 is provided with an opening for the sample mixing device to extend into. The opening of the motion card slot 21 also extends in the radial direction of the sample loading cover 2, which can increase the length for the sample mixing device to extend into, and improve the adaptability to the sample mixing device. The motion card slot 21 extends in the radial direction and has a large contact area, which is convenient for the sample mixing device to fully contact the motion card slot 21 after entering the motion card slot 21, and improves the stability of the sample mixing device and the motion card slot 21. In addition, the motion card slot 21 is located at the top of the sample loading cover 2, which is convenient for the connection operation of the sample mixing device, and can effectively improve the operating efficiency of reagent mixing. In another embodiment, the opening of the motion slot 21 away from the base 1 does not extend radially along the sample loading cover 2, and the sample mixing device can be inserted into the motion slot 21 through the openings at both ends of the motion slot 21 in the radial direction, and then the locking piece is inserted into the opening, and the sample mixing device is fixed by the locking piece, thereby improving the stability of the sample mixing device and the motion slot 21. The sample loading cover 2 is made of injection molding material.

[0039] like Figure 1 and Figure 2 As shown, in this embodiment, the motion slot 21 includes an insertion section 211 and a limiting section 212. The opening of the insertion section 211 is set at the top of the insertion section 211. The limiting section 212 is connected to the bottom end of the insertion section 211, the cross-sectional size of the insertion section 211 is smaller than the cross-sectional size of the limiting section 212, and the bottom surface of the insertion section 211 is used to abut against the limiting sample mixing device. The sample mixing device can switch between a normal state and an expanded state, and the width of the sample mixing device in the expanded state is greater than the width of the sample mixing device in the normal state. During the process of the sample mixing device being adapted and engaged with the motion slot 21, first, the sample mixing device in a normal state enters the limiting section 212 through the insertion section 211, and at this time, the sample mixing device switches from a normal state to an expanded state. Since the cross-sectional size of the insertion section 211 is smaller than the cross-sectional size of the limiting section 212, the sample mixing device will be engaged with the limiting section 212, and the protrusions formed on the sample loading cover 2 on both sides of the insertion section 211 cooperate to limit the sample mixing device, thereby ensuring the stability of the adaptation and engagement between the sample mixing device and the motion slot 21.

[0040] like Figure 1 and Figure 2As shown, in this embodiment, both ends of the slot sidewalls of the insertion section 211 and the limiting section 212 along the length direction are provided with guiding slopes 213, and the guiding slopes 213 are inclined from the outer surface of the sample loading cover 2 to the slot sidewalls, and the guiding slopes 213 are used to guide the sample mixing device into the motion slot 21. The sample mixing device can enter the motion slot 21 through the opening of the motion slot 21, or can be inserted into the motion slot 21 through the openings at both ends of the motion slot 21 in the radial direction. At this time, the guiding slopes 213 formed on the sidewalls of the insertion section 211 and the limiting section 212 can facilitate the positioning and matching of the sample mixing device, which is conducive to the sample mixing device smoothly entering the motion slot 21.

[0041] In one embodiment, a magnetic suction part for adsorbing magnetic beads is provided on the sample loading cover 2. Among them, a magnetic bead hole for accommodating magnetic beads is also provided on the base 1. The sample loading cover 2 is first rotated to align the sample loading hole 22 with the magnetic bead hole, and the nucleic acid substance is injected. Then, the sample loading cover 2 is rotated to adsorb the magnetic beads through the magnetic suction part, and the nucleic acid substance is attached to the magnetic beads. Then, the sample loading cover 2 is driven to rotate axially so that the sample loading hole 22 is aligned with the storage bin, and the magnetic beads are adsorbed by the magnetic suction part, and then the sample loading cover 2 is continued to rotate to realize the transfer of the nucleic acid substance, so that the nucleic acid substance is transported by the magnetic suction part, and reacts with the reagents in multiple storage bins on the base 1 in turn, and finally the detection of the nucleic acid substance is completed.

[0042] like Figure 3 As shown, in another embodiment, a magnetic hole 23 is provided on the side of the sample cover 2 facing the base 1, and the magnetic element is sealed in the magnetic hole 23, and the magnetic hole 23 does not pass through the sample cover 2. Preferably, the diameter of the magnetic element is slightly smaller than the aperture of the magnetic hole 23, and a filling gap is left between the magnetic element and the magnetic hole 23, and the filling gap can be used for the injection of sealant, so that the magnetic element is fixed in the magnetic hole 23 by the sealant, ensuring the connection stability of the magnetic element. Of course, the magnetic element can also be fixed in the magnetic hole 23 by interference fit, and other methods that can fix the magnetic element are all acceptable. Among them, the diameter of the magnetic element matches the diameter of the storage bin to prevent the liquid in the storage bin from overflowing when the sample detection cartridge is flipped.

[0043] like Figure 3As shown, in the embodiment of the utility model, the sample loading cover 2 is also provided with a transfer hole 24 on one side facing the base 1, and one end of the transfer hole 24 is open and used for docking with the receiving bin. Similarly, the transfer hole 24 does not penetrate the sample loading cover 2. The transfer hole 24 is used to transfer the liquid in the receiving bin. After the transfer hole 24 is docked with the receiving bin, the sample detection card box is flipped by the sample mixing device. At this time, the base 1 is above the sample loading cover 2, so that the liquid in the receiving bin flows into the transfer hole 24. At this time, the sample mixing device drives the sample loading cover 2 to rotate relative to the card box until it docks with another receiving bin. After mixing with the liquid in all the receiving bins in turn, the sample detection card box is flipped by the sample mixing device. At this time, the sample loading cover 2 is above the base 1. Of course, the sample detection card box can be flipped multiple times during the mixing process of the receiving bin and the liquid in the transfer hole 24 to ensure that the liquid in the receiving bin and the transfer hole 24 can be fully mixed.

[0044] like Figure 3 As shown, in this embodiment, the pipetting hole 24 is arranged near the sample loading hole 22, with a compact layout and centralized arrangement, which reduces the space occupied by multiple holes in the sample loading cover 2. Among them, the sample loading hole 22 is also sealed with a hole cover 3, which improves the sealing of the sample detection card box, avoids the contamination of nucleic acid substances due to poor sealing effect of the sample detection card box, reduces the risk of cross infection, and improves the accuracy of the detection results of the sample detection card box. Among them, a card hole is also provided at the center position on the base 1, and a plurality of storage bins are arranged around the periphery of the card hole. A buckle 4 that passes through the card hole is provided on the top cover. The buckle 4 is sealed with the card hole through the seal, which further improves the sealing effect of the sample detection card box, and also improves the connection stability between the sample loading cover 2 and the base 1.

[0045] like Figure 3 As shown, in this embodiment, the centers of the pipetting hole 24, the sample adding hole 22 and the magnetic suction hole 23 are located on the same radius arc. The pipetting hole 24, the sample adding hole 22 and the magnetic suction hole 23 are arranged circumferentially on the sample adding cover 2, and are arranged around the outer periphery of the buckle 4. The sample adding hole 22 is arranged between the magnetic suction hole 23 and the pipetting hole 24. The pipetting hole 24, the sample adding hole 22 and the magnetic suction hole 23 can be docked with the storage bin on the base 1 only by rotating. Preferably, the spacing between the sample adding hole 22 and the magnetic suction hole 23 is twice the spacing between the sample adding hole 22 and the pipetting hole 24. Other arrangements of the pipetting hole 24, the sample adding hole 22 and the magnetic suction hole 23 can also be adjusted accordingly according to the arrangement of the magnetic bead hole and the storage bin on the base 1.

[0046] Furthermore, the spacing between the magnetic hole 23 and the transfer hole 24 is set correspondingly according to the spacing between different storage bins on the base 1, and the spacing between the sample loading hole 22 and the magnetic hole 23 can also be adjusted according to the spacing between the magnetic bead hole and the storage bin on the base 1. Among them, the upper side wall surface of the sample loading cover 2 is also provided with a limiting protrusion 6 corresponding to the magnetic hole 23, and the limiting protrusion 6 is used to slide and contact with the rotating rail provided on the side wall of the base 1, so that the sample loading cover 2 can rotate more stably relative to the base 1 under the drive of the sample mixing device.

[0047] like Figure 3 As shown, in the embodiment of the utility model, the motion slot 21 is arranged in the middle of the sample loading cover 2 and divides the sample loading cover 2 into two top cover parts of the same shape, and the pipetting hole 24, the sample loading hole 22 and the magnetic suction hole 23 are all arranged on the top cover part on either side of the motion slot 21, with a compact layout, which improves the space utilization rate of the sample loading cover 2. Among them, the base 1 is also provided with a rotation limit slot, and the side wall of the sample loading cover 2 is provided with a rotation protrusion 5, which is used to cooperate with the rotation limit slot and a notch is provided above the rotation protrusion 5, and the notch is provided for the rotation protrusion 5 to be elastically deformed, so as to better cooperate with the rotation limit slot, so that the rotation protrusion 5 is not easy to slide after being engaged with the rotation limit slot, which can effectively limit the technical problem that the sample loading cover 2 of the sample detection cartridge is easy to rotate relative to the base 1 after leaving the factory.

[0048] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0049] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0051] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A sample detection cartridge, characterized in that: The sample detection cartridge comprises: A base (1) is provided with a plurality of storage compartments; A sample loading cover (2) and a sealing cover are mounted on the base (1); the sample loading cover (2) is provided with a moving slot (21) for engaging with a sample mixing device; the sample loading cover (2) is used to rotate relative to the base (1) under the drive of the sample mixing device; the sample loading cover (2) is provided with a sample loading hole (22) for communicating with the outside; and the plurality of storage bins are used to communicate with the sample loading cover (2).

2. The sample detection cartridge according to claim 1, characterized in that: The movement slot (21) is opened at the top of the sample loading cover (2) and passes through the sample loading cover (2) in the radial direction of the sample loading cover (2). An opening is provided on a side of the movement slot (21) away from the base (1).

3. The sample detection cartridge according to claim 2, characterized in that: The motion card slot (21) comprises: An insertion section (211), wherein the opening is arranged at a top end of the insertion section (211); A limiting section (212), wherein the limiting section (212) is connected to the bottom end of the inserting section (211), the cross-sectional dimension of the inserting section (211) is smaller than the cross-sectional dimension of the limiting section (212), and the bottom surface of the inserting section (211) is used to abut against and limit the sample mixing device.

4. The sample detection cartridge according to claim 3, characterized in that: Both ends of the slot side walls of the insertion section (211) and the limiting section (212) along the length direction are provided with guiding inclined surfaces (213), the guiding inclined surfaces (213) being arranged obliquely from the outer side of the sample loading cover (2) toward the slot side walls, and the guiding inclined surfaces (213) are used to guide the sample mixing device into the moving card slot (21).

5. The sample detection cartridge according to any one of claims 1 to 4, characterized in that: The sample adding cover (2) is provided with a magnetic attraction component for absorbing magnetic beads.

6. The sample detection cartridge according to claim 5, characterized in that: A magnetic attraction hole (23) is provided on one side of the sample loading cover (2) facing the base (1), and the magnetic attraction component is sealed in the magnetic attraction hole (23).

7. The sample detection cartridge according to claim 6, characterized in that: The sample loading cover (2) is also provided with a transfer hole (24) on one side facing the base (1); one end of the transfer hole (24) is open and is used for docking with the storage bin.

8. The sample detection cartridge according to claim 7, characterized in that: The pipetting hole (24) is arranged close to the sample adding hole (22).

9. The sample detection cartridge according to claim 8, characterized in that: The centers of the pipetting hole (24), the sample adding hole (22) and the magnetic suction hole (23) are located on an arc with the same radius.

10. The sample detection cartridge according to claim 9, characterized in that: The motion slot (21) is arranged in the middle of the sample loading cover (2) and divides the sample loading cover (2) into two top cover parts of the same shape, and the pipetting hole (24), the sample loading hole (22) and the magnetic suction hole (23) are all arranged on the top cover part on either side of the motion slot (21).

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