Magnetic attraction structure, sample processing device and gene processing equipment

Abstract

The utility model relates to a magnetic attraction structure, a sample processing device and gene processing equipment. The magnetic attraction structure comprises a mounting plate; the magnetic parts are arranged on one side of the mounting plate at intervals, each magnetic part is provided with two different pole ends, and the ends, close to each other, of the adjacent magnetic parts are arranged to be the same pole; and at least partially surrounded magnetic suction areas are formed at the joints of the homopolar end parts of the adjacent magnetic pieces and the mounting plate. In the application, the magnetic attraction area of the magnetic attraction structure is increased, and the adjacent magnetic parts are fixedly mounted on the mounting plate in a homopolar opposite manner, so that the gathering range of the magnetic beads is expanded, the magnetic beads are more strongly influenced by magnetic force and are not easily taken away by liquid, and the magnetic attraction efficiency of the sample is further improved.

Description

Technical Field

[0001] The present application relates to the field of magnetic attraction technology, and in particular to a magnetic attraction structure, a sample processing device and a gene processing equipment. Background Art

[0002] The magnetic bead method is one of the main methods for achieving nucleic acid extraction. When using the magnetic bead method for nucleic acid extraction, the magnetic beads need to be separated and retained. In the existing magnetic suction structure, after the pipette absorbs the liquid with magnetic beads, the driving component drives the component equipped with the magnet frame to move in the direction of the pipette, so that the magnet on the component is close to one side of the pipette tip. Under the influence of the magnetic force, the magnetic beads approach the magnet and finally stop on the inner wall of the tip. After the magnetic beads are completely adsorbed, the pipette is controlled to slowly inject liquid to achieve the function of separating the magnetic beads from the liquid.

[0003] In the existing magnetic attraction structure, a single magnet is used for magnetic attraction. The magnetic force of a single magnet is relatively weak and the magnetic attraction area is relatively small. When a single magnet is close to the suction tip, the magnetic beads are gathered on the inner wall of the suction tip in a limited range and the magnetic beads are stacked on the inner wall of the suction tip. When the pipette is injected with liquid, the water flow is easy to carry away some of the top magnetic beads that are weakly affected by the magnetic force. When the magnetic beads are gathered and stacked, too much liquid remains between the magnetic bead particles, which is not conducive to subsequent drying and other processing steps. Secondly, a single magnet is prone to misalignment and offset problems when close to the suction tip, which is not easy to correct. The above reasons will affect the magnetic attraction efficiency and lead to a decrease in the magnetic attraction efficiency. Utility Model Content

[0004] The purpose of the present application is to provide a magnetic attraction structure, a sample processing device and a gene processing equipment. In the present application, the magnetic attraction area of ​​the magnetic attraction structure is increased, and adjacent magnetic parts are fixedly mounted on a mounting plate in a manner where the same poles are opposite to each other. The range of magnetic bead aggregation is expanded, and the magnetic beads are more strongly affected by the magnetic force and are not easily carried away by the liquid, thereby further improving the magnetic attraction efficiency of the sample.

[0005] The embodiment of the present application is implemented as follows:

[0006] In a first aspect, the present application provides a magnetic attraction structure, comprising: a mounting plate; a plurality of magnetic parts, which are arranged at intervals on one side of the mounting plate, and the magnetic parts have two different extreme ends, and the ends of adjacent magnetic parts that are close to each other are set as the same pole; wherein the connection between the same pole ends of adjacent magnetic parts and the mounting plate forms a magnetic attraction area that is at least partially enclosed.

[0007] In the above technical solution, the magnetic attraction area of ​​the magnetic attraction structure is increased, and adjacent magnetic parts are fixed on the mounting plate with the same poles facing each other. The range of magnetic bead aggregation is expanded, the magnetic beads are more strongly affected by the magnetic force, and are less likely to be carried away by the liquid, thereby further improving the magnetic attraction efficiency of the sample.

[0008] In one embodiment, a first step is provided on the mounting plate, and a groove is provided at the first step; a second step is provided on the magnetic member, and the second step matches the groove, so that the mounting plate and the magnetic member are embedded with each other.

[0009] In the above technical solution, the mounting plate and the magnetic member are interlocked with each other, which can further enhance the connection stability between the magnetic member and the mounting plate.

[0010] In one embodiment, the magnetic attraction structure further includes: a pressing plate, one end of the pressing plate is connected to the mounting plate, and the other end of the pressing plate is connected to the magnetic member.

[0011] In the above technical solution, the magnetic component is further fixedly mounted on the mounting plate by a pressing plate, which can improve the stability of the mounting structure of the magnetic component and improve the situation where the magnetic component falls off during the magnetic attraction process.

[0012] In one embodiment, the pressure plate includes a first pressure plate portion and a second pressure plate portion; the first pressure plate portion is connected to the mounting plate, one end of the second pressure plate portion is connected to the first pressure plate portion, and one end of the second pressure plate portion is connected to the magnetic member.

[0013] In the above technical solution, the pressing plate is divided into two parts, the first pressing plate part is used to connect the mounting plate and play a supporting role, and the second pressing plate part is used to connect the magnetic part and play a fixing role. The magnetic part is fixedly mounted on the mounting plate through the joint action of the first pressing plate part and the second pressing plate part.

[0014] In one embodiment, the first pressure plate portion includes: a first pressure plate straight portion and a first pressure plate arc portion; wherein, the first pressure plate straight portion is arranged parallel to the mounting plate, the first pressure plate arc portion is connected to the bottom of the first pressure plate straight portion, and the first pressure plate arc portion is bent and extended in the setting direction of the first step; an arc surface extending in the setting direction of the first step is provided on the surface of the first step near the first pressure plate arc portion.

[0015] In the above technical solution, the arc portion of the first pressure plate is set as an arc structure, which can improve the situation where the connection between the first pressure plate portion and the second pressure plate portion is at a right angle and collides with the first step of the mounting plate. Secondly, the arc surface set on the surface of the first step can also improve the situation where the arc portion of the first pressure plate collides with the first step of the mounting plate.

[0016] In one embodiment, the second pressure plate portion includes: a second pressure plate straight portion, a second pressure plate arc portion and a third pressure plate straight portion; the second pressure plate straight portion is connected to the first pressure plate portion, the second pressure plate straight portion is arranged parallel to one of the surfaces of the magnetic member, one end of the second pressure plate arc portion is connected to the second pressure plate straight portion, the other end of the second pressure plate arc portion is connected to the third pressure plate straight portion, and the third pressure plate straight portion is connected to the magnetic member.

[0017] In the above technical solution, the arc portion of the second pressing plate is set as an arc structure, which can improve the situation where the connection between the straight portion of the second pressing plate and the straight portion of the third pressing plate collides with the edge of the magnetic part. In addition, the setting of the arc portion of the second pressing plate can also prevent the consumables from colliding with the pressing plate when approaching the magnetic attraction structure, thereby damaging the consumables and affecting the experimental results.

[0018] In one embodiment, the magnetic attraction structure further includes: a magnetic attraction driving unit, wherein an output end of the magnetic attraction driving unit is connected to the mounting plate for driving the mounting plate to move.

[0019] In the above technical solution, the mounting plate can be driven by the magnetic drive unit to achieve reciprocating movement, so that the magnetic structure can be moved according to experimental requirements.

[0020] In one embodiment, the magnetic attraction structure further includes: a substrate and a sliding component; the magnetic attraction driving part is installed on the substrate, the sliding component is installed on the substrate, and the sliding component is connected to the mounting plate.

[0021] In the above technical solution, the sliding component can limit the position of the mounting plate during forward or backward movement, thereby improving the offset of the mounting plate during movement.

[0022] In one embodiment, the sliding assembly includes: a slider and a slide rail, the slide rail is arranged on the base plate, one end of the slider is connected to the mounting plate, and one end of the slider is slidably arranged on the slide rail.

[0023] In the above technical solution, the sliding assembly adopts a structure of a slider and a slide rail. The slide rail can support and limit the movement of the slider, which can improve the stability of the mounting plate when it moves linearly. The setting of the slide rail can transfer part of the overturning torque to the slide rail, and under the support force provided by the slide rail, the overall stability of the magnetic attraction structure is improved.

[0024] In one embodiment, the sliding block includes: a supporting portion and a sliding connection portion; the supporting portion is connected to the mounting plate, the sliding connection portion is connected to the supporting portion, and the sliding connection portion is slidably disposed on the slide rail.

[0025] In the above technical solution, the support part plays the role of supporting and dragging the mounting plate, and the sliding connection part plays the role of guiding the sliding. Through the matching structure of the slider and the slide rail, the forward or backward linear movement of the magnetic attraction structure is realized.

[0026] In a second aspect, the present application provides a sample processing device, comprising a frame, a pipetting structure, and a magnetic structure as described in any embodiment of the first aspect of the present application; the pipetting structure and the magnetic structure are both arranged on the frame; the pipetting structure is configured to be able to move to the magnetic structure, and the magnetic structure is configured to perform a magnetic operation on the sample transferred by the pipetting structure.

[0027] In the above technical solution, the sample processing device can ensure that the magnetic beads in the sample are still within the magnetic attraction range of the magnetic attraction structure while the liquid transfer structure moves up and down. The requirement for whether the sample enters the magnetic attraction area in parallel is low, and the operation difficulty is reduced.

[0028] In a third aspect, the present application provides a gene processing device, comprising a sample processing device as described in the embodiment of the second aspect of the present application.

[0029] In the above technical solution, the gene processing equipment can complete experiments including nucleic acid processing, PCR amplification, fluorescence reaction, etc., and has a wider application. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 A schematic diagram of a magnetic attraction structure provided in an embodiment of the present application Figure 1 ;

[0032] Figure 2 A diagram showing the experimental results of magnetic attraction of a magnetic member provided in an embodiment of the present application in a manner where the same poles are arranged opposite to each other;

[0033] Figure 3 A diagram showing the experimental results of magnetic attraction of a magnetic member provided in an embodiment of the present application in a manner where opposite poles are arranged to each other;

[0034] Figure 4 A front view of a magnetic attraction structure provided in one embodiment of the present application;

[0035] Figure 5 A left side view of a magnetic attraction structure provided in one embodiment of the present application;

[0036] Figure 6 for Figure 5 The enlarged schematic diagram at A in the middle;

[0037] Figure 7 A top view of a magnetic attraction structure provided in one embodiment of the present application;

[0038] Figure 8 A schematic diagram of a magnetic attraction structure provided in an embodiment of the present application Figure 2 ;

[0039] Fig. 9 A schematic diagram of the structure of a sample processing device provided in an embodiment of the present application Figure 1 ;

[0040] Fig.10 A front view of a sample processing device provided in an embodiment of the present application Figure 1 ;

[0041] Fig.11 A left view of a sample processing device provided in one embodiment of the present application Figure 1 ;

[0042] Fig.12 A schematic diagram of the structure of a sample processing device provided in an embodiment of the present application Figure 2 ;

[0043] Fig.13 A front view of a sample processing device provided in an embodiment of the present application Figure 2 ;

[0044] Fig.14 A left view of a sample processing device provided in one embodiment of the present application Figure 2 .

[0045] icon:

[0046] 1-sample processing device; 11-magnetic structure; 100-mounting plate; 110-first step; 111-groove; 112-arc surface; 200-magnetic member; 210-second step; 300-magnetic area; 400-pressing plate; 410-first pressing plate portion; 411-first pressing plate straight portion; 412-first pressing plate arc portion; 420-second pressing plate portion; 421-second pressing plate straight portion; 422-second pressing plate arc portion; 423-third pressing plate straight portion; 500-magnetic drive portion; 600-substrate; 610-photoelectric detection unit; 611-photoelectric detection portion; 612-light shielding sheet; 700-sliding assembly; 710-sliding block ;711-supporting part;712-sliding connecting part;720-slide rail;12-frame;121-top plate;122-bottom plate;13-pipetting structure;131-piston rod rack guide rod;132-piston bracket;133-piston cylinder;134-accommodating chamber;135-piston rod;136-tip mounting part;137-pipetting drive member;138-tip ejection plate;139-tip ejection elastic guide rod;14-magnetic structure;141-magnetic rod rack guide rod;142-magnetic rod bracket;143-magnetic rod;144-magnetic sleeve mounting part;145-magnetic rod drive member;146-demagnetization rod sleeve plate;147-demagnetization rod sleeve elastic guide rod. DETAILED DESCRIPTION

[0047] The terms “first”, “second”, “third”, etc. are only used for distinguishing descriptions and do not indicate the order of arrangement, nor can they be understood as indicating or implying relative importance.

[0048] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0049] In the description of the present application, it should be noted that the terms "inside", "outside", "left", "right", "up", "down", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the products of the present application are usually placed when in use. They are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present application.

[0050] In the description of this application, unless otherwise clearly specified and limited, the terms "set", "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements.

[0051] The technical solution of the embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0052] Please refer to Figure 1 The present application provides a magnetic attraction structure 11, including: a mounting plate 100, a plurality of magnetic members 200, wherein the magnetic members 200 are arranged at intervals on one side of the mounting plate 100, the magnetic members 200 have two different extreme ends, and the ends of adjacent magnetic members 200 that are close to each other are set as the same pole; wherein the connection between the same pole ends of adjacent magnetic members 200 and the mounting plate 100 forms a magnetic attraction area 300 that is at least partially surrounded.

[0053] In this embodiment, the magnetic member 200 may be a magnet having an N pole and an S pole. Each magnetic member 200 is arranged at intervals, and adjacent magnetic members 200 are fixedly mounted on the mounting plate 100 by welding or the like in a manner that the same poles face each other (or the same poles repel each other). The areas where the same poles of two magnetic members 200 face each other and the connection area of ​​the mounting plate 100 form a magnetic attraction area 300 surrounded by at least three sides, and the two sides corresponding to the magnetic members 200 in the magnetic attraction area 300 are magnetic areas.

[0054] Please refer to Figure 2 When the consumables such as the pipette tip or test tube containing the magnetic bead sample are moved into the magnetic attraction area 300, the magnetic beads in the consumables are affected by the repulsive magnetic force of the magnetic member 200 with the same polarity in the magnetic attraction area 300, and the magnetic beads approach the end of the magnetic member 200 close to the consumables, and are finally stopped on both sides of the inner wall of the consumables after being adsorbed by both sides. There is a flow channel in the middle of the consumables for the sample liquid to pass through. After the magnetic beads are completely adsorbed, the sample in the consumables is slowly injected to separate the magnetic beads and the liquid.

[0055] Please refer to Figure 3 If adjacent magnetic members 200 are fixedly mounted on the mounting plate 100 in a manner of oppositely disposed opposite poles, when the suction head loaded with a sample containing magnetic beads moves into the magnetic attraction area 300, there are a large number of magnetic beads. After the magnetic beads are attracted by the magnets of the magnetic members 200 on both sides, the magnetic beads will gather together to form a magnet with an N pole and an S pole. The N pole of the magnetic beads is attracted to the S pole of the magnetic member 200, and the S pole of the magnetic beads is attracted to the N pole of the magnetic member 200. Some magnetic beads will be connected in the middle of the suction head. When liquid is injected, the liquid is likely to carry away the middle magnetic beads, thereby affecting the magnetic attraction effect.

[0056] Through experimental tests, it was found that adjacent magnetic parts 200 were fixed on the mounting plate 100 in a manner where like poles faced each other (or like poles repelled each other), the range of magnetic bead aggregation was expanded, the magnetic beads were more strongly affected by the magnetic force, and were less likely to be carried away by the liquid, thereby further improving the magnetic attraction efficiency of the sample and making the magnetic attraction effect more significant.

[0057] In some embodiments, the mounting plate 100 may be a plate-shaped or block-shaped material having magnetic properties. When adjacent magnetic members 200 are fixedly mounted on the mounting plate 100 with the same poles facing each other, the areas where the same poles of the two magnetic members 200 face each other and the connection area of ​​the mounting plate 100 form a magnetic attraction area 300 surrounded on three sides, so that each surface is magnetic, thereby increasing the magnetic area of ​​the magnetic area, thereby increasing the magnetic force, allowing the magnetic beads to be adsorbed on multiple sides, and improving the magnetic attraction efficiency.

[0058] In some embodiments, the magnetic member 200 may be in a square, rectangular, cylindrical, etc. In this embodiment, the magnetic member 200 is in a rectangular shape.

[0059] For further information, please refer to Figure 4 , Figure 5 , Figure 6 , Figure 7 A first step 110 is provided on the mounting plate 100, and a groove 111 is provided at the first step 110; a second step 210 is provided on the magnetic member 200, and the second step 210 matches the groove 111, so that the mounting plate 100 and the magnetic member 200 are embedded with each other.

[0060] In this embodiment, the mounting plate 100 and the magnetic member 200 are interlocked with each other, which can further enhance the connection stability between the magnetic member 200 and the mounting plate 100 .

[0061] Furthermore, the magnetic attraction structure 11 further includes: a pressing plate 400 , one end of the pressing plate 400 is connected to the mounting plate 100 , and the other end of the pressing plate 400 is connected to the magnetic member 200 .

[0062] In this embodiment, based on the mutual interlocking of the mounting plate 100 and the magnetic component 200, the magnetic component 200 is further fixed on the mounting plate 100 by the pressing plate 400, which can improve the stability of the mounting structure of the magnetic component 200 and improve the situation where the magnetic component 200 falls off during the magnetic attraction process.

[0063] Optional, see Figure 6 The pressing plate 400 includes a first pressing plate portion 410 and a second pressing plate portion 420 ; the first pressing plate portion 410 is connected to the mounting plate 100 , one end of the second pressing plate portion 420 is connected to the first pressing plate portion 410 , and one end of the second pressing plate portion 420 is connected to the magnetic member 200 .

[0064] In this embodiment, the pressing plate 400 is provided in two parts, the first pressing plate part 410 is used to connect the mounting plate 100 and play a supporting role, and the second pressing plate part 420 is used to connect the magnetic member 200 and play a fixing role. Through the joint action of the first pressing plate part 410 and the second pressing plate part 420, the magnetic member 200 is fixedly mounted on the mounting plate 100.

[0065] Optionally, a portion of the first pressing plate portion 410 and the second pressing plate portion 420 may be hollowed out, which can save material of the pressing plate 400 and reduce costs, and at the same time will not affect the fixed installation of the magnetic member 200.

[0066] Furthermore, the first pressure plate portion 410 includes: a first pressure plate straight portion 411 and a first pressure plate arc portion 412; wherein, the first pressure plate straight portion 411 is connected to the mounting plate 100, the first pressure plate arc portion 412 is connected to the bottom of the first pressure plate straight portion 411, and the first pressure plate arc portion 412 bends and extends in the setting direction of the first step 110; an arc surface 112 extending in the setting direction of the first step 110 is provided on the surface of the first step 110 near the first pressure plate arc portion 412.

[0067] In this embodiment, the first pressing plate arc portion 412 is configured as an arc structure, which can improve the situation where the first pressing plate portion 410 and the second pressing plate portion 420 collide and interfere with the first step 110 of the mounting plate 100 when the connection point is at a right angle. Secondly, the arc surface 112 provided on the surface of the first step 110 can also improve the situation where the first pressing plate arc portion 412 collides with the first step 110 of the mounting plate 100.

[0068] The second pressure plate portion 420 includes: a second pressure plate straight portion 421, a second pressure plate arc portion 422 and a third pressure plate straight portion 423; the second pressure plate straight portion 421 is connected to the first pressure plate arc portion 412, the second pressure plate straight portion 421 is arranged parallel to one of the surfaces of the magnetic member 200, one end of the second pressure plate arc portion 422 is connected to the second pressure plate straight portion 421, the other end of the second pressure plate arc portion 422 is connected to the third pressure plate straight portion 423, and the third pressure plate straight portion 423 is connected to the magnetic member 200.

[0069] In this embodiment, the second pressing plate arc portion 422 is configured as an arc structure, which can improve the situation where the connection between the second pressing plate straight portion and the third pressing plate straight portion 423 collides with the edge of the magnetic member 200. In addition, the configuration of the second pressing plate arc portion 422 can also prevent the consumables from colliding with the pressing plate 400 when approaching the magnetic attraction structure 11, thereby damaging the consumables and affecting the experimental results.

[0070] In the above embodiment, the magnetic member 200 is fixed on the mounting plate 100 by using the structure of the pressing plate 400. In other embodiments, an adhesive installation method may also be used, that is, the magnetic member 200 is glued to the mounting plate 100.

[0071] For further information, please refer to Figure 8 The magnetic attraction structure 11 further includes: a magnetic attraction driving unit 500, the output end of the magnetic attraction driving unit 500 is connected to the mounting plate 100, and is used to drive the mounting plate 100 to move.

[0072] In this embodiment, the mounting plate 100 can be driven by the magnetic driving unit 500 to achieve reciprocating movement, so that the magnetic structure 11 can move according to experimental requirements.

[0073] In some embodiments, the magnetic drive unit 500 may be a screw motor, which converts the rotational motion of the motor into linear motion, and then drives the mounting plate 100 forward or backward through the forward and reverse rotation of the screw motor, so that the magnetic component 200 moves closer to or away from the consumables. In other embodiments, the magnetic drive unit 500 may also be a cylinder, which is directly connected to the mounting plate 100 through the output end of the cylinder, and drives the mounting plate 100 forward or backward through the telescopic movement of the cylinder, thereby driving the magnetic component 200 closer to or away from the consumables.

[0074] Furthermore, the magnetic attraction structure 11 also includes: a substrate 600 and a sliding assembly 700 ; the magnetic attraction driving unit 500 is installed on the substrate 600 , the sliding assembly 700 is installed on the substrate 600 , and the sliding assembly 700 is connected to the mounting plate 100 .

[0075] In this embodiment, the sliding assembly 700 can limit the mounting plate 100 during forward or backward movement, thereby improving the offset of the mounting plate 100 during movement.

[0076] Furthermore, the sliding assembly 700 includes: a slider 710 and a slide rail 720 . The slide rail 720 is disposed on the base plate 600 . One end of the slider 710 is connected to the mounting plate 100 , and one end of the slider 710 is slidably disposed on the slide rail 720 .

[0077] In this embodiment, the sliding assembly 700 adopts the structure of a slider 710 and a slide rail 720. The slide rail 720 can support and limit the movement of the slider 710, thereby improving the stability of the mounting plate 100 when performing linear motion. In addition, the setting of the slide rail 720 can transfer part of the overturning moment to the slide rail 720, and under the support force provided by the slide rail 720, the overall stability of the magnetic attraction structure 11 is improved.

[0078] Optionally, the slider 710 includes: a supporting portion 711 and a sliding connecting portion 712 ; the supporting portion 711 is connected to the mounting plate 100 , the sliding connecting portion 712 is connected to the supporting portion 711 , and the sliding connecting portion 712 is slidably disposed on the slide rail 720 .

[0079] In this embodiment, the support portion 711 serves to support and drag the mounting plate 100, and the sliding connection portion 712 serves to guide the sliding. Through the matching structure of the slider 710 and the slide rail 720, the forward or backward linear motion of the magnetic attraction structure 11 is realized.

[0080] For further information, please refer to Figure 8A photoelectric detection unit 610 is also provided on the substrate 600, and the photoelectric detection unit 610 includes a photoelectric detection part 611 and a light blocking sheet 612; the photoelectric detection part 611 is fixedly arranged on the substrate 600, and the photoelectric detection part 611 is a slot-type photoelectric switch with a groove, and the light blocking sheet 612 is arranged on the side of the supporting part 711, and the photoelectric detection part 611 is used to identify and detect the light blocking sheet 612.

[0081] In summary, the magnetic attraction structure 11 provided in the present application improves the single magnet attraction in the prior art into multi-directional and multi-quantity magnetic attraction, thereby increasing the magnetic attraction area, expanding the range of magnetic bead aggregation, and allowing the magnetic beads located on the top layer of the sample to be more strongly affected by the magnetic force and less likely to be carried away by the liquid during injection, thereby improving the magnetic attraction efficiency.

[0082] The magnetic attraction structure 11 of the present application can be used in conjunction with a liquid transfer structure having a liquid transfer function. Fig. 9 The present application provides a sample processing device 1, comprising: Figure 1-Figure 8 The magnetic structure 11, the frame 12, and the pipetting structure 13 are shown; the pipetting structure 13 and the magnetic structure 11 are both arranged on the frame 12; the pipetting structure 13 is configured to be able to move to the magnetic structure 11, and the magnetic structure 11 is configured to perform magnetic suction operations on the samples transferred by the pipetting structure 13.

[0083] Optional, see Fig.10 , Fig.11 The liquid transfer structure 13 includes: a piston rod frame guide rod 131, a piston bracket 132, a plurality of piston cylinders 133, a plurality of piston rods 135, a suction head mounting portion 136 and a liquid transfer driving member 137. The piston rod frame guide rod 131 connects the top plate 121 and the bottom plate 122 of the frame 12, the piston bracket 132 is sleeved on the piston rod frame guide rod 131 and is located between the top plate 121 and the bottom plate 122, and the piston bracket 132 can slide up and down along the piston rod frame guide rod 131. A plurality of through holes are provided on the bottom plate 122, and the piston cylinder 133 is provided in the through holes, and each piston cylinder 133 has a accommodating chamber 134; one end of the piston rod 135 is connected to the piston bracket 132, and the other end can extend into the accommodating chamber 134 of the piston cylinder 133; a suction head mounting portion 136 is provided on the bottom plate 122 for mounting a suction head, and a vent hole is provided in the suction head mounting portion 136, and the vent hole is connected to the bottom of the piston cylinder 133; a liquid transfer driving component 137 is provided on the top plate 121, and the output end of the liquid transfer driving component 137 is transmission-connected to the piston bracket 132, and is used to drive the piston bracket 132 to rise and fall.

[0084] Optionally, the pipetting structure 13 further includes: a head ejection plate 138 and a head ejection elastic guide rod 139, wherein the head ejection plate 138 is movably disposed on the head mounting portion 136; the head ejection elastic guide rod 139 is disposed on the head ejection plate 138, and a head ejection elastic guide rod through hole is disposed on the bottom plate 122, and the head ejection elastic guide rod 139 passes through the head ejection elastic guide rod through hole and extends toward the piston bracket 132. The piston bracket 132 is driven to move downward by the pipetting driving member 137, and the piston bracket 132 pushes the head ejection elastic guide rod 139 downward, thereby causing the head ejection elastic guide rod 139 to push the head ejection plate 138 downward, and the head mounted on the head mounting portion 136 is unloaded by the force of the head ejection plate 138.

[0085] The specific structure of the pipetting structure 13 can refer to the specific structural description of the pipetting module in the patent with application number 202211405808.9 and title "Gene Detection Processing Equipment and Method", which will not be repeated here. Among them, the pipetting structure 13 can be installed on the rack through a vertical lifting module, and the structure of the vertical lifting module can still refer to the specific structural description of the vertical lifting module in the patent with application number 202211405808.9 and title "Gene Detection Processing Equipment and Method".

[0086] In other embodiments, the specific structure and working principle of the pipetting structure 13 can also refer to the description of the specific structure and working principle of the pipetting module in the patent with application number 202210375663.6 and titled "Gene Detection Device", which will not be repeated here.

[0087] In some embodiments, see Fig.12 , Fig.13 , Fig.14 The sample processing device 1 may further include: a magnetic attraction structure 14; the magnetic attraction structure 14 includes: a magnetic rod frame guide rod 141, a magnetic rod bracket 142, a plurality of magnetic rods 143, a magnetic sleeve mounting portion 144, and a magnetic rod driving member 145. The magnetic rod frame guide rod 141 connects the top plate 121 and the bottom plate 122 of the frame 12, the magnetic rod bracket 142 is sleeved on the magnetic rod frame guide rod 141 and is located between the top plate 121 and the bottom plate 122, the magnetic rod bracket 142 can slide up and down along the magnetic rod frame guide rod 141, the bottom plate 122 is provided with a plurality of through holes, one end of the plurality of magnetic rods 143 is connected to the magnetic rod bracket 142, and the other end can pass through the through hole. A magnetic sleeve mounting portion 144 for mounting a magnetic rod sleeve is provided on the bottom plate 122. The magnetic sleeve mounting portion 144 is designed as a hollow structure to allow the magnetic rod 143 to pass through. A magnetic rod driving component 145 is provided on the top plate 121. The output end of the magnetic rod driving component 145 is transmission-connected to the magnetic rod bracket 142 for driving the magnetic rod bracket 142 to move up and down.

[0088] Optionally, the magnetic attraction structure 14 further includes: a demagnetizing rod sleeve plate 146 and a demagnetizing rod sleeve elastic guide rod 147. The demagnetizing rod sleeve plate 146 is movably arranged on the magnetic sleeve mounting portion 144, and the demagnetizing rod sleeve elastic guide rod 147 is arranged on the demagnetizing rod sleeve plate 146. A demagnetizing rod sleeve elastic guide rod through hole is also arranged on the bottom plate 122, and the demagnetizing rod sleeve elastic guide rod 147 passes through the demagnetizing rod sleeve elastic guide rod through hole and extends to the magnetic rod bracket 142. The magnetic rod bracket 142 is driven to move downward by the magnetic rod driving member 145, and the magnetic rod bracket 142 pushes the demagnetizing rod sleeve elastic guide rod 147 downward, thereby causing the demagnetizing rod sleeve elastic guide rod 147 to push the demagnetizing rod sleeve plate 146 downward, and the magnetic rod sleeve installed on the magnetic sleeve mounting portion 144 is unloaded by the force of the demagnetizing rod sleeve plate 146.

[0089] The specific structure of the magnetic attraction structure 14 can refer to the specific structural description of the magnetic attraction module and working principle in the patent with application number 202211405808.9 and titled "Gene Detection Processing Equipment and Method", which will not be repeated here.

[0090] In this embodiment, the magnetic attraction structure 14 can be used synchronously with the magnetic attraction structure 11, or it can be used independently of the magnetic attraction structure 11. The magnetic attraction structure 14 or the magnetic attraction structure 11 is configured to perform magnetic bead adsorption operation according to the actual experimental requirements.

[0091] In some embodiments, the sample processing device 1 further includes: a membrane piercing structure, which is controlled to pierce the sealing film on the surface of the reagent kit and other consumables. The specific structure of the membrane piercing structure can refer to the specific structural description of the membrane piercing module in the patent application number 202211405808.9, entitled "Gene Detection Processing Equipment and Method", which will not be repeated here.

[0092] The working principle of the sample processing device 1 of the present application is as follows:

[0093] First, the sample is sucked and injected through the liquid transfer structure 13, and the liquid transfer drive 137 drives the piston support 132 to rise, and the multiple piston rods 135 connected to the piston support 132 are pulled out from the piston cylinder 133 upward, and the sample with magnetic beads in the container or reagent box is extracted into the suction head by using the same principle as the syringe. After the liquid transfer structure 13 absorbs the sample with magnetic beads, the liquid transfer structure 13 is controlled to move downward by the vertical lifting module, so that the liquid transfer structure 13 can move to the position of the magnetic attraction structure 11. At this time, the magnetic attraction drive unit 500 is controlled to start, and the magnetic attraction drive unit 500 drives the mounting plate 100 to move to the position of the suction head of the liquid transfer structure 13, so that the suction head on the suction head mounting part 136 of the liquid transfer structure 13 enters the magnetic attraction area 300 in the magnetic attraction structure 11, so as to ensure that the sample containing magnetic beads can be located in the magnetic attraction area 300 surrounded by three sides formed by the area with the same poles of the magnetic member 200 and the connection area of ​​the mounting plate 100. At this time, the magnetic attraction driving unit 500 is controlled to stop, and the magnetic beads in the tip are affected by the repulsive magnetic force of the magnetic parts 200 with like poles repelling each other in the magnetic attraction area 300. The magnetic beads approach the end of the magnetic part 200 close to the consumables, and are adsorbed by both sides and finally stop on both sides of the inner wall of the tip. There is a flow channel in the middle of the tip for the sample liquid to pass through.

[0094] After the magnetic beads are completely adsorbed, the piston bracket 132 is driven down by the pipetting drive 137, and the multiple piston rods 135 connected to the piston bracket 132 are pushed downward from the piston cylinder 133 to inject the sample in the pipette tip into the sample processing container to separate the magnetic beads from the liquid.

[0095] In order to further fully separate the liquid in the tip from the magnetic beads, the vertical lifting module can be used to drive the pipetting structure 13 to rise a short distance, so that the magnetic beads partially resting on the inner wall of the tip slide downward under the influence of the magnetic force, thereby achieving the purpose of spreading the magnetic beads, further reducing the amount of magnetic beads remaining in the liquid, and improving the magnetic suction efficiency.

[0096] After the magnetic attraction is completed, the vertical lifting module is used to control the transfer structure 13 to move upward, so that the transfer structure 13 leaves the magnetic attraction structure 11, and the magnetic attraction driving unit 500 drives the mounting plate 100 to move, so that the magnetic attraction structure 11 is reset.

[0097] In summary, the sample processing device 1 of the present application can ensure that the magnetic beads in the sample are still within the magnetic attraction range of the magnetic attraction structure 11 while the pipetting structure 13 moves up and down. Even if the suction head is not parallel to the magnetic attraction structure 11, it can still be ensured that the suction head is within the magnetic attraction range of the magnetic attraction area 300, so the requirement for whether the sample enters the magnetic attraction area 300 in parallel is relatively low, and the operation difficulty is reduced.

[0098] Furthermore, the present application also provides a gene processing device, including: Figure 8-Figure 13The sample processing device 1 shown in the figure can complete experiments including nucleic acid processing, PCR amplification, fluorescence reaction, etc., and has a wider application.

[0099] It should be noted that, in the absence of conflict, the features in the embodiments of this application may be combined with each other.

[0100] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A magnetic attraction structure, characterized in that: include: Mounting plate; A plurality of magnetic members are arranged at intervals on one side of the mounting plate, the magnetic members have two different extreme ends, and the ends of the adjacent magnetic members close to each other are arranged to be of the same pole; Wherein, the connection between the pole ends of the adjacent magnetic parts and the mounting plate forms a magnetic attraction area which is at least partially surrounded.

2. The magnetic attraction structure according to claim 1, characterized in that: The mounting plate is provided with a first step, and a groove is provided at the first step; the magnetic member is provided with a second step, and the second step matches the groove, so that the mounting plate and the magnetic member are embedded with each other.

3. The magnetic attraction structure according to claim 2, characterized in that: The magnetic attraction structure further includes: a pressing plate, one end of which is connected to the mounting plate, and the other end of which is connected to the magnetic member.

4. The magnetic attraction structure according to claim 3, characterized in that: The pressing plate includes a first pressing plate portion and a second pressing plate portion; the first pressing plate portion is connected to the mounting plate, one end of the second pressing plate portion is connected to the first pressing plate portion, and one end of the second pressing plate portion is connected to the magnetic member.

5. The magnetic attraction structure according to claim 4, characterized in that: The first pressure plate portion includes: a first pressure plate straight portion and a first pressure plate arc portion; wherein, the first pressure plate straight portion is connected to the mounting plate, the first pressure plate arc portion is connected to the bottom of the first pressure plate straight portion, and the first pressure plate arc portion is bent and extended in the setting direction of the first step; a curved surface extending in the setting direction of the first step is provided on the surface of the first step near the first pressure plate arc portion.

6. The magnetic attraction structure according to claim 4 or 5, characterized in that: The second pressure plate portion includes: a second pressure plate straight portion, a second pressure plate arc portion and a third pressure plate straight portion; the second pressure plate straight portion is connected to the first pressure plate portion, the second pressure plate straight portion is arranged parallel to one of the surfaces of the magnetic component, one end of the second pressure plate arc portion is connected to the second pressure plate straight portion, the other end of the second pressure plate arc portion is connected to the third pressure plate straight portion, and the third pressure plate straight portion is connected to the magnetic component.

7. The magnetic attraction structure according to claim 1, characterized in that: The magnetic attraction structure further includes: a magnetic attraction driving unit, wherein an output end of the magnetic attraction driving unit is connected to the mounting plate for driving the mounting plate to move.

8. The magnetic attraction structure according to claim 7, characterized in that: The magnetic attraction structure further includes: a base plate and a sliding assembly; the magnetic attraction driving part is installed on the base plate, the sliding assembly is installed on the base plate, and the sliding assembly is connected to the mounting plate.

9. The magnetic attraction structure according to claim 8, characterized in that: The sliding assembly comprises: a slider and a slide rail, wherein the slide rail is arranged on the base plate, one end of the slider is connected to the mounting plate, and one end of the slider is slidably arranged on the slide rail.

10. The magnetic attraction structure according to claim 9, characterized in that: The sliding block comprises: a supporting portion and a sliding connection portion; the supporting portion is connected to the mounting plate, the sliding connection portion is connected to the supporting portion, and the sliding connection portion is slidably arranged on the slide rail.

11. A sample processing device, characterized in that: It comprises a frame, a pipetting structure, and a magnetic structure as described in any one of claims 1 to 10; the pipetting structure and the magnetic structure are both arranged on the frame; the pipetting structure is configured to be able to move to the magnetic structure, and the magnetic structure is configured to perform a magnetic operation on the sample transferred by the pipetting structure.

12. A gene processing device, characterized in that: Comprising the sample processing device of claim 11.

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