Weak current stimulation stem cell culture device

Abstract

The utility model provides a culture device for stem cells stimulated by weak current, which belongs to the technical field of stem cell culture and comprises a culture dish body. The support assembly comprises multiple supports, multiple limiting sliding blocks, multiple limiting sliding grooves, an annular track, an annular frame and two sliding rods, the multiple supports are arranged on the circumferential surface of the culture dish body, the multiple limiting sliding grooves are formed in the multiple supports respectively, and the annular track is arranged on the annular frame. The multiple limiting sliding blocks are slidably connected into the multiple limiting sliding grooves correspondingly, the annular rail is fixedly connected to the surfaces of the multiple limiting sliding blocks, and the annular frame is slidably connected into the annular rail. By using the device, the position of the weak-current electrode in the culture dish main body is convenient to adjust, the weak-current electrode is convenient to use to provide a weak-current environment for stem cell culture at different positions in the culture dish main body, and the device is convenient to operate and use.

Description

Technical Field

[0001] The utility model belongs to the technical field of stem cell culture, and in particular relates to a culture device for weak-electrically stimulating stem cells. Background Art

[0002] Stem cells, also known as "seed cells", are a type of cell with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated daughter cells; stem cells have the characteristics of self-renewal, unlimited proliferation and multidirectional differentiation potential; according to their developmental potential, stem cells can be divided into three categories: totipotent stem cells, pluripotent stem cells, and unipotent stem cells; according to their developmental stage, they can be divided into two categories: embryonic stem cells and adult stem cells; theoretically, stem cells can be used for clinical cell transplantation to treat various diseases and construct artificial tissues or organs, and the suitable diseases include: tissue necrosis diseases such as myocardial necrosis caused by ischemia, degenerative lesions such as Parkinson's syndrome, autoimmune diseases such as rheumatoid arthritis, diabetes, bone and joint diseases, etc.; but most of the research is still in the clinical trial stage, and the only routine operation is hematopoietic stem cell transplantation to treat blood system diseases;

[0003] In the prior art, to culture stem cells in a laboratory, the stem cells need to be placed in a culture dish. The stem cells proliferate in a suitable environment, and weak electrical stimulation is used to increase the rapid growth of mesenchymal stem cells. Usually, two conductive electrodes are placed in the culture dish to add a continuous weak electrical stimulation to the environment in the culture dish. The electrodes are placed in the culture dish and are not easy to adjust. It is not convenient to use the electrodes to perform weak electrical culture of stem cells at different positions in the culture dish. Utility Model Content

[0004] The utility model aims to provide a culture device for weak electric stimulation of stem cells, aiming to solve the problem that in the prior art, when culturing stem cells in a laboratory, the stem cells need to be placed in a culture dish, and the stem cells proliferate in a suitable environment, and weak electric stimulation is used to increase the rapid growth of mesenchymal stem cells. Usually, two conductive electrodes are placed in the culture dish to add a continuous weak electric stimulation to the environment in the culture dish. The electrodes are placed in the culture dish and are not easy to adjust, and it is not convenient to use the electrodes to perform weak electric culture of stem cells at different positions in the culture dish.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A culture device for weakly electrically stimulating stem cells, comprising:

[0007] Petri dish body;

[0008] A bracket assembly, the bracket assembly includes a bracket, a limiting slide block, a limiting slide groove, an annular track, an annular frame and a slide rod, the bracket, the limiting slide groove and the limiting slide block are all provided in plurality, the slide rod is provided with two, the plurality of the brackets are all provided on the circumferential surface of the culture dish body, the plurality of the limiting slide grooves are respectively opened in the plurality of brackets, the plurality of the limiting slide blocks are respectively slidably connected in the plurality of limiting slide grooves, the annular track is fixedly connected to the surface of the plurality of limiting slide blocks, the annular frame is slidably connected in the annular track, and the two brackets are fixedly connected to the circumferential inner wall of the annular frame;

[0009] The weak current components are provided with two groups, each group of the weak current components comprises a T-block, a weak current electrode and a transmission line, the T-block is slidably connected to the surfaces of two sliding rods, the weak current electrode is fixedly connected to the lower end of the T-block, and the transmission line is fixedly connected to the upper end of the T-block.

[0010] As a preferred solution of the utility model, a plurality of positioning holes are provided on the surfaces of the plurality of brackets, a plurality of positioning nails are slidably connected inside the plurality of limit sliders, and the plurality of positioning nails match the plurality of positioning holes.

[0011] As a preferred solution of the utility model, the two transmission lines are respectively connected to a weak current power source.

[0012] As a preferred solution of the utility model, a plurality of push rods are fixedly connected to the upper end of the annular frame.

[0013] As a preferred solution of the utility model, the length of the weak current electrode is 10 cm.

[0014] As a preferred solution of the present invention, the height of the plurality of brackets is 15 cm.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. In this solution, by using this device, the position of the weak-current electrode in the culture dish body is easy to adjust, so that the weak-current electrode can be used to provide a weak-current environment for stem cell culture at different positions in the culture dish body, which is convenient to operate and use.

[0017] 2. In this scheme, a weak-current electrode is fixedly connected to the lower end of the T-block. The T-block slides on the surface of the slide rod, and the annular frame rotates in the annular track to adjust the position of the lower end of the weak-current electrode in the culture dish body. The weak-current electrode outputs weak electricity, which acts on the culture dish body to provide a weak-current environment for stem cell culture.

[0018] 3. In this solution, four brackets effectively maintain the stability between the culture dish and the electrode.

[0019] 4. In this solution, the adjustable slide bar at the top can adjust the distance between the weak-electric electrodes to meet the needs of different electric field stimulations.

[0020] 5. In this scheme, the height of the weak current electrode shaft can also be adjusted. The height of the lower electrode can be adjusted according to the height of the culture medium to adapt to and ensure that the electrode does not contact the flat layer of cells.

[0021] 6. In this solution, multiple adjustable devices can meet the requirements of different incubators and different electric fields and adapt to the adjustment of various culture parameters. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0023] Figure 1 It is a three-dimensional diagram of the utility model;

[0024] Figure 2 It is the front view of the utility model;

[0025] Figure 3 It is a top view of the utility model;

[0026] Figure 4 It is a partial stereogram of the utility model.

[0027] In the figure: 1. Culture dish body; 2. Bracket; 3. Limiting slide groove; 4. Limiting slider; 5. Positioning hole; 6. Positioning pin; 7. Annular track; 8. Annular frame; 9. Push rod; 10. Slide rod; 11. T-block; 12. Weak current electrode; 13. Transmission line. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] Example

[0030] See also Figure 1-Figure 4 , the utility model provides the following technical solutions:

[0031] A culture device for weakly electrically stimulating stem cells, comprising:

[0032] Petri dish body 1;

[0033] A bracket assembly, the bracket assembly includes a bracket 2, a limiting slide block 4, a limiting slide groove 3, an annular track 7, an annular frame 8 and a slide bar 10. The bracket 2, the limiting slide groove 3 and the limiting slide block 4 are all provided with multiple, and the slide bar 10 is provided with two. The multiple brackets 2 are all arranged on the circumferential surface of the culture dish body 1, and the multiple limiting slide grooves 3 are respectively opened in the multiple brackets 2. The multiple limiting slide blocks 4 are respectively slidably connected to the multiple limiting slide grooves 3. The annular track 7 is fixedly connected to the surface of the multiple limiting slide blocks 4, and the annular frame 8 is slidably connected in the annular track 7. The two brackets 2 are fixedly connected to the circumferential inner wall of the annular frame 8;

[0034] There are two groups of weak current components, each group of weak current components includes a T-block 11, a weak current electrode 12 and a transmission line 13. The T-block 11 is slidably connected to the surfaces of the two sliding rods 10, the weak current electrode 12 is fixedly connected to the lower end of the T-block 11, and the transmission line 13 is fixedly connected to the upper end of the T-block 11.

[0035] In a specific embodiment of the utility model, the annular track 7 in the bracket assembly is arranged on the upper side of the culture dish body 1, a limited slide groove 3 is provided in the bracket 2, and a limited slider 4 is slidably connected in the limited slide groove 3. A plurality of positioning holes 5 are provided on the surface of each bracket 2, and a hole matching the positioning hole 5 is provided in the limiting slider 4. The limiting slider 4 slides in the limiting slide groove 3 to adjust its height. After the adjustment is completed, the positioning pin 6 is inserted into the positioning hole 5 and the limiting slider 4 to complete the positioning of the limiting slider 4. The limiting slider 4 is fixed to the annular track 7, and an annular frame 8 is rotatably connected in the annular track 7. The slide rod 10 is fixed to the inner wall of the annular frame 8. The T-block 11 in the component slides on the surfaces of the two slide bars 10, and the lower end of the T-block 11 is fixedly connected with a weak-current electrode 12. The T-block 11 slides on the surface of the slide bar 10, and the annular frame 8 rotates in the annular track 7 to adjust the position of the lower end of the weak-current electrode 12 in the culture dish body 1. The weak-current electrode 12 outputs weak electricity, and the weak electricity acts on the culture dish body 1 to provide a weak-current environment for stem cell culture. By using the device, the position of the weak-current electrode 12 in the culture dish body 1 is easy to adjust, and the weak-current electrode 12 is convenient to use to provide a weak-current environment for stem cell culture at different positions in the culture dish body 1, which is convenient to operate and use.

[0036] For details, please refer to Figure 1-Figure 4 A plurality of positioning holes 5 are provided on the surfaces of the plurality of brackets 2 , and a plurality of positioning pins 6 are slidably connected inside the plurality of limit sliders 4 , and the plurality of positioning pins 6 match the plurality of positioning holes 5 .

[0037] In this embodiment: the positioning hole 5 is used to connect the positioning pin 6. After the limit slider 4 slides to a suitable position in the limit slide groove 3, the positioning pin 6 is inserted into the limit slider 4 and the positioning hole 5 at the horizontal position of the limit slider 4 to complete the positioning of the limit slider 4.

[0038] For details, please refer to Figure 1-Figure 4 , the two transmission lines 13 are respectively connected to the weak current power source.

[0039] In this embodiment, the weak current power source outputs weak current after passing through the transmission line 13, the T-block 11 and the weak current electrode 12.

[0040] For details, please refer to Figure 1-Figure 4 A plurality of push rods 9 are fixedly connected to the upper end of the annular frame 8 .

[0041] In this embodiment, a plurality of push rods 9 are provided to facilitate the annular frame 8 to rotate in the annular track 7 .

[0042] For details, please refer to Figure 1-Figure 4 , the length of the weak current electrode 12 is 10 cm.

[0043] In this embodiment, the length of the weak current electrode 12 is set to 10 cm, and the lower end of the weak current electrode 12 is a weak current output end.

[0044] For details, please refer to Figure 1-Figure 4 , the height of the multiple brackets 2 is 15 cm.

[0045] In this embodiment, the height of the bracket 2 is 15 cm, and the bracket 2 plays the role of supporting the annular frame 8.

[0046] It should be noted that the specific type of transmission line 13 and weak current electrode 12 to be used is selected by relevant technicians familiar with the field, and the above transmission line 13 and weak current electrode 12 are all existing technologies and will not be elaborated in this solution.

[0047] The working principle and use process of the utility model are as follows: when the device is in use, the bracket assembly is arranged on the surface of the culture dish body 1. When a weak current is needed at a position in the culture dish body 1, the control push rod 9 drives the annular frame 8 to rotate in the annular track 7, and the T-block 11 slides on the surface of the two sliding rods 10 to adjust the position of the weak current electrode 12. After adjusting the position of the weak current electrode 12, the stem cells in the culture dish body 1 are weakly stimulated. By using the device, the position of the weak current electrode 12 in the culture dish body 1 is easy to adjust, and the weak current electrode 12 is convenient to use to provide a weak current environment for stem cell culture at different positions in the culture dish body 1, which is convenient to operate and use.

[0048] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A culture device for weak electrical stimulation of stem cells, characterized in that: include: Petri dish body (1); A support assembly, the support assembly comprising a support (2), a limiting slide block (4), a limiting slide groove (3), an annular track (7), an annular frame (8) and a slide bar (10), wherein the support (2), the limiting slide groove (3) and the limiting slide block (4) are each provided in plurality, and the slide bar (10) is provided in two. The plurality of the supports (2) are each provided on the circumferential surface of the culture dish body (1), the plurality of the limiting slide grooves (3) are respectively provided in the plurality of the supports (2), the plurality of the limiting slide blocks (4) are respectively slidably connected in the plurality of limiting slide grooves (3), the annular track (7) is fixedly connected to the surface of the plurality of limiting slide blocks (4), the annular frame (8) is slidably connected in the annular track (7), and the two supports (2) are each fixedly connected to the circumferential inner wall of the annular frame (8); A weak current component is provided in two groups, each group of the weak current components comprises a T-shaped block (11), a weak current electrode (12) and a transmission line (13), the T-shaped block (11) is slidably connected to the surfaces of two sliding bars (10), the weak current electrode (12) is fixedly connected to the lower end of the T-shaped block (11), and the transmission line (13) is fixedly connected to the upper end of the T-shaped block (11).

2. A weak electrical stimulation stem cell culture device according to claim 1, characterized in that: A plurality of positioning holes (5) are provided on the surfaces of the plurality of brackets (2), and a plurality of positioning pins (6) are slidably connected inside the plurality of limit sliders (4), and the plurality of positioning pins (6) match the plurality of positioning holes (5).

3. A weak electrical stimulation stem cell culture device according to claim 2, characterized in that: The two transmission lines (13) are respectively connected to a weak current power source.

4. A weak electrical stimulation stem cell culture device according to claim 3, characterized in that: A plurality of push rods (9) are fixedly connected to the upper end of the annular frame (8).

5. The weakly electrically stimulated stem cell culture device according to claim 4, characterized in that: The length of the weak current electrode (12) is 10 cm.

6. The weakly electrically stimulated stem cell culture device according to claim 5, characterized in that: The height of the plurality of brackets (2) is 15 cm.

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