Strain culture device for polysaccharide production

Abstract

The utility model provides a strain culture device for polysaccharide production. The strain culture device comprises a tank body, a liquid outlet is formed in the bottom of the tank body, a ventilation mechanism is arranged at the top of the tank body, and a stirring mechanism and a bubble breaking mechanism are arranged in the tank body; the bubble breaking mechanism comprises a hollow shaft and a cross rod arranged at the bottom end of the hollow shaft, the hollow shaft is rotationally installed at the top of the tank body through an outer gear bearing, a rotating shaft penetrates out of the hollow shaft, a second motor is arranged outside the top of the tank body, and the second motor is in transmission connection with the outer gear bearing through a gear transmission mechanism arranged in the tank body. The position of the cross rod corresponds to the position of the liquid level of a polysaccharide strain culture solution in the tank body, and a plurality of pricking needles are arranged on the cross rod. According to the utility model, the stirring mechanism and the bubble breaking mechanism operate independently, the stirring mechanism stirs and mixes polysaccharide strains and a culture solution for fermentation, so that the fermentation uniformity and speed are improved, and the bubble breaking mechanism performs defoaming operation on metabolic bubbles generated by fermentation, so that the polysaccharide strains are prevented from being poisoned by metabolic gas, and the culture quality of the strains is further ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of polysaccharide fermentation, and particularly relates to a strain culture device for polysaccharide production. Background Art

[0002] During the fermentation and culture process of polysaccharide strains, gases such as carbon dioxide and hydrogen are generated during the interaction between the strains and the culture solution. These gases are released into the culture solution to form bubbles that gather together, and then foam is generated. Secondly, the fermentation raw materials often contain some substances that are prone to foaming, such as proteins and polysaccharides. These substances will be decomposed and utilized by microorganisms during the fermentation process, and then foam is generated.

[0003] The bubbles generated by the strain fermentation have hazards such as reducing the effective area of fermentation, reducing the utilization rate of strains, and poisoning the strains. Therefore, whether the bubbles generated by the strain fermentation can be eliminated directly relates to the quality of strain fermentation.

[0004] To solve the above problems, at present, some strain fermentation culture devices are provided with a thorn needle defoaming structure on the stirring blades of the stirring mechanism. When the stirring mechanism is started, the strains and the culture solution are mixed. Along with the action of the stirring mechanism, the thorns pierce the generated bubbles for defoaming. This structure makes the defoaming action must depend on the stirring action. The purpose of the stirring operation is to mix the strains and the culture solution evenly at the initial stage of cultivation, which is helpful for the fermentation and culture of the strains. It is best to carry out static fermentation culture in the middle and later stages of cultivation to ensure the activity of the strains. During this stage, a large amount of bubbles are generated by the metabolism of the strains, forming foam on the surface of the culture solution, floating on the culture liquid surface and gathering near the inner side wall of the tank. Therefore, defoaming operation needs to be carried out at this stage. If the above-mentioned thorn needle defoaming structure is used for defoaming operation, on the one hand, it will stir the strain mixture, which will inevitably affect the activity of the strains and the fermentation process. On the other hand, it cannot achieve targeted defoaming, that is, it cannot effectively defoam the foam gathered on the surface of the culture solution, affecting the defoaming effect. Summary of the Utility Model

[0005] In view of this, the utility model aims to provide a strain culture device for polysaccharide production, which effectively defoams the bubbles generated by the fermentation of polysaccharide strains gathered on the culture liquid surface, ensures the smooth progress of the fermentation of polysaccharide strains, and ensures the quality of strain culture.

[0006] To achieve the above object, the technical solution of the utility model is realized as follows:

[0007] A strain culture device for polysaccharide production includes a tank body. The bottom of the tank body is provided with a liquid outlet, and the top is provided with a ventilation mechanism. A stirring mechanism and a foam-breaking mechanism are arranged in the tank body;

[0008] The stirring mechanism comprises a rotating shaft, the lower end of which extends to the bottom of the tank body and is provided with stirring blades, and the rotating shaft extends out of the top of the tank body and matches the first motor;

[0009] The bubble breaking mechanism includes a hollow shaft and a cross bar arranged at the bottom end thereof. The hollow shaft is rotatably mounted on the top of the tank body through an external gear bearing. The rotating shaft passes through the hollow shaft. A second motor is arranged outside the top of the tank body. The second motor is connected to the external gear bearing through a gear transmission mechanism arranged in the tank body. The position of the cross bar corresponds to the liquid level position of the polysaccharide bacteria culture liquid in the tank body, and the cross bar is arranged with a plurality of needles.

[0010] Furthermore, a scraper is provided at the far end of the cross bar, and the outer edge of the scraper abuts against the inner wall of the tank body.

[0011] Furthermore, the scraper is an arc-shaped plate, and its concave surface is the water-facing surface.

[0012] Furthermore, the scraper is a resin scraper.

[0013] Furthermore, the cross bar is provided with the needles on its water-facing side, upper side and lower side respectively.

[0014] Furthermore, the ventilation mechanism includes a ventilation pipe arranged on the top of the tank body, and a control valve is provided on the ventilation pipe.

[0015] Furthermore, a cover shell is provided outside the tank body, an annular cavity is formed between the cover shell and the tank body, and an inlet and an outlet communicating with the annular cavity are provided at the upper ends of the left and right sides of the cover shell.

[0016] Compared with the prior art, the bacterial strain culture device for polysaccharide production described in the utility model has the following advantages:

[0017] (1) The utility model provides a stirring mechanism and a foam breaking mechanism in a polysaccharide bacteria fermentation tank, and the stirring mechanism and the foam breaking mechanism operate independently. Before fermentation, the stirring mechanism stirs and mixes the polysaccharide bacteria and the fermentation culture solution to increase the fermentation speed of the bacteria. The foam breaking mechanism operates in the later stage of fermentation to defoam the bubbles generated by fermentation metabolism and floating on the surface of the culture solution, thereby improving the defoaming effect and enabling the polysaccharide bacteria to ferment normally, thereby ensuring the culture quality of the bacteria.

[0018] (2) In the utility model, a scraper is provided on the cross bar. The scraper is an arc-shaped plate, and its concave surface is the water-facing surface, so that the foam collected near the side wall of the tank body can be transferred to the center of the tank body, making it easier for the pricking needles on the cross bar to break the bubbles, thereby improving the defoaming speed and effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which form a part of the present utility model, are used to provide a further understanding of the present utility model. The schematic embodiments and descriptions thereof of the present utility model are used to explain the present utility model and do not constitute an improper limitation to the present utility model. In the drawings:

[0020] Figure 1 Schematic diagram of a strain culturing device for polysaccharide production according to an embodiment of the present utility model;

[0021] Figure 2 Front view of the foam-breaking mechanism in an embodiment of the present utility model;

[0022] Figure 3 Top view of the foam-breaking mechanism in an embodiment of the present utility model.

[0023] Explanation of reference numerals:

[0024] 1 - Tank body; 2 - Housing; 21 - Inlet; 22 - Outlet; 3 - Rotating shaft; 31 - Stirring blade; 4 - Liquid outlet; 5 - Hollow shaft; 51 - Cross bar; 52 - Scraper; 53 - Prickly needle; 54 - Flange; 6 - Outer gear bearing; 7 - Second motor; 8 - First motor; 9 - Vent pipe; 10 - Annular cavity. Detailed implementation manners

[0025] The present utility model will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0026] As Figure 1 shown, a strain culturing device for polysaccharide production includes a tank body 1 for fermentation and culturing of polysaccharide strains. The bottom of the tank body 1 is a downwardly convex spherical structure, and a liquid outlet 4 is provided at the bottom of the tank. A ventilation mechanism and an observation window are provided at the top of the tank body 1. A stirring mechanism and a foam-breaking mechanism are provided inside the tank body 1. At the same time, a thermometer (not shown in the figure) for detecting the temperature of the culture solution is provided inside the tank body 1, and a pressure gauge (not shown in the figure) for detecting the gas pressure inside the tank is provided above the liquid level of the culture solution inside the tank body 1. In the present utility model, the tank body 1 adopts a split structure, including an upper tank body and a lower tank body that are sealed and connected by screws. The upper tank body is provided with a hoisting structure to facilitate the hoisting and movement of the upper tank body. The stirring mechanism and the foam-breaking mechanism are both installed on the upper tank body.

[0027] The stirring mechanism operates before the strain fermentation, mixing the strains and the culture solution placed in the tank body 1. It includes a first motor 8 and a rotating shaft 3. The first motor 8 is installed on the top surface of the tank body 1, and the rotating shaft 3 is arranged at the center of the tank body 1. Its top end extends out of the top of the tank body 1 and is connected to the main shaft of the first motor 8. The lower end of the rotating shaft 3 extends to the bottom of the tank body 1 and is provided with a stirring blade 31. The stirring mechanism stirs the polysaccharide strains and the culture solution inside the tank body 1 to make them mix evenly, improving the strain culturing speed.

[0028] The foam breaking mechanism operates in the late fermentation stage to defoam the foam generated during the fermentation and cultivation of the strains. It includes a hollow shaft 5 and a cross bar 51 arranged at its bottom end. The cross bar 51 is provided with a pricking needle 53. The position of the cross bar 51 corresponds to the position of the liquid level of the polysaccharide strain culture liquid in the tank body in the late fermentation stage to prevent the pricking needle 53 on the cross bar from damaging the strains. The hollow shaft 5 is also arranged at the center of the tank body 1. The hollow shaft 5 is rotatably mounted on the top of the tank body 1 through an external gear bearing 6, and the rotating shaft 3 passes through the hollow shaft 5. A second motor 7 is arranged outside the top of the tank body 1, and a gear transmission mechanism corresponding to the external gear bearing 6 is arranged inside the tank body 1. The second motor 7 and the external gear bearing 6 are transmission-connected through the gear transmission mechanism.

[0029] The specific structure of the bubble breaking mechanism is as follows Figure 2 , Figure 3 As shown, a flange 54 for connecting with the external gear bearing 6 is provided at the top of the hollow shaft 5, two cross bars 51 are provided and symmetrically arranged on the left and right sides of the hollow shaft 5, a scraper 52 corresponding to the side wall of the tank body 1 is provided at the far end of the cross bar 51, and a plurality of needles 53 are respectively arranged on the upward side, the downward side and the water-facing side of the cross bar 51.

[0030] A large amount of foam is generated in the middle and late stages of the fermentation of the polysaccharide strains in the tank body, and is gathered near the inner wall of the tank body 1. The outer edge of the scraper 52 abuts against the inner wall of the tank body 1, and the rotation of the hollow shaft 5 drives the scraper 52 to move. The scraper 52 scrapes the inner wall of the tank body 1 and transfers the foam gathered near the inner wall of the tank body 1 to the center of the tank body, so that the needle 53 on the cross bar 51 can pierce the bubbles and perform defoaming operations to prevent the metabolic gases generated by the fermentation of the strains from poisoning the polysaccharide strains, thereby ensuring that the polysaccharide strains can ferment normally and the culture quality of the strains is guaranteed.

[0031] In the present invention, the scraper 52 is an arc-shaped resin scraper, and its concave surface is the water-facing surface. The outer edge of the scraper 52, that is, the end thereof abutting against the inner wall of the tank body, is located in front of the cross bar 52 along the circumference and the rotation direction of the hollow shaft, that is, when the hollow shaft 5 rotates, the cross bar 51 always moves behind the outer edge of the scraper 5. Since the foam generated by the fermentation of the strains is mostly gathered near the inner wall of the tank body, the arc-shaped scraper structure facilitates the transfer of the foam gathered on the inner wall of the tank body to the center of the tank body 1, so that the needle 53 on the cross bar 51 following closely behind can pierce the bubbles, thereby improving the defoaming effect.

[0032] In the utility model, the ventilation mechanism includes a ventilation pipe 9 arranged on the top of the tank body 1, and a control valve (not shown in the attached drawings) is arranged on the ventilation pipe 9. The tank body 1 is provided with a pressure gauge above the liquid level of the culture solution. When the gas pressure generated during the fermentation process is greater than the set pressure, the control valve is opened to discharge the gas in the tank body 1. In order to prevent the gas from polluting the environment, the ventilation pipe 9 is connected to a filtering mechanism to filter harmful substances in the discharged gas to ensure that the discharged gas is clean gas.

[0033] In the present utility model, a housing 2 is provided outside the tank body 1. An annular cavity 10 is formed between the housing 2 and the tank body 1. An inlet 21 and an outlet 22 communicating with the annular cavity 10 are provided at the upper positions on the left and right sides of the housing 2. High-temperature steam or hot water is injected into the annular cavity 10 through the inlet 21, flows in the annular cavity 10, and exchanges heat with the tank body 1, so that the bacteria in the tank body 1 are in the temperature environment required for fermentation, ensuring the fermentation operation.

[0034] The above are only the preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A bacterial strain culture device for polysaccharide production, characterized in that: The tank body (1) comprises a tank body (1), wherein a liquid outlet (4) is provided at the bottom of the tank body (1) and a ventilation mechanism is provided at the top; and a stirring mechanism and a bubble breaking mechanism are provided inside the tank body (1); The stirring mechanism comprises a rotating shaft (3), the lower end of the rotating shaft (3) extends to the bottom of the tank body (1) and is provided with a stirring blade (31), and the top end of the rotating shaft (3) extends out of the top of the tank body (1) and is matched with a first motor (8); The bubble breaking mechanism comprises a hollow shaft (5) and a cross bar (51) arranged at the bottom end thereof; the hollow shaft (5) is rotatably mounted on the top of the tank body (1) via an external gear bearing (6); the rotating shaft (3) passes through the hollow shaft (5); a second motor (7) is arranged outside the top of the tank body (1); the second motor (7) is connected to the external gear bearing (6) via a gear transmission mechanism arranged inside the tank body (1); the position of the cross bar (51) corresponds to the position of the liquid level of the polysaccharide bacterial culture solution in the tank body (1); and the cross bar (51) is provided with a plurality of pricking needles (53).

2. A bacterial strain culture device for polysaccharide production according to claim 1, characterized in that: A scraper (52) is provided at the far end of the cross bar (51), and the outer edge of the scraper (52) abuts against the inner wall of the tank body (1).

3. A bacterial strain culture device for polysaccharide production according to claim 2, characterized in that: The scraper (52) is an arc-shaped plate, and its concave surface is the water-facing surface.

4. A bacterial strain culture device for polysaccharide production according to claim 2, characterized in that: The scraper (52) is a resin scraper.

5. The bacterial strain culture device for polysaccharide production according to claim 1, characterized in that: The cross bar (51) is provided with the needles (53) on its water-facing side, upper side and lower side respectively.

6. The bacterial strain culture device for polysaccharide production according to claim 1, characterized in that: The ventilation mechanism comprises a ventilation pipe (9) arranged on the top of the tank body (1), and a control valve is provided on the ventilation pipe (9).

7. The bacterial strain culture device for polysaccharide production according to claim 1, characterized in that: A cover shell (2) is provided outside the tank body (1), an annular cavity (10) is formed between the cover shell (2) and the tank body (1), and an inlet (21) and an outlet (22) communicating with the annular cavity (10) are provided at the upper ends of the left and right sides of the cover shell (2).

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