Full-automatic whole blood collection and preparation machine with matched disposable device

Abstract

The utility model is suitable for the technical field of whole blood collection and preparation devices, and provides a full-automatic whole blood collection and preparation machine with a matched disposable device, which comprises a full-automatic whole blood collection and preparation machine and a disposable collection and preparation device, the full-automatic whole blood collecting and preparing machine comprises a control system, a pipeline light sensor, a pipeline pressure monitor, a weighing device, a pipeline bubble monitor, a centrifugal machine system, a valve, an air pressure cuff and a peristaltic pump, the disposable collecting and preparing device is provided with a centrifugal cup, a blood bag, a pipeline and the like, and the pipeline comprises a whole blood pipeline for blood matching and the like. According to the full-automatic whole blood collecting and preparing machine with the matched disposable device, through the collecting and preparing method, a certain amount of peripheral venous blood of a blood donor can be collected and mixed with a certain proportion of blood maintenance liquid to form whole blood, meanwhile, the whole blood can be separated into red blood cells, plasma and albuginea, and the separated red blood cells can be used for collecting and preparing the whole blood. The washing red blood cells can be further prepared on the equipment.

Description

Technical Field

[0001] The utility model belongs to the technical field of whole blood collection and preparation devices, in particular to a full-automatic whole blood collection and preparation machine with a matching disposable device. Background Art

[0002] Blood is an important component of the body and plays a variety of important physiological functions. Blood is composed of red blood cells, white blood cells, platelets and plasma. Blood usually refers to whole blood collected from qualified blood donors and mixed with a certain proportion of blood maintenance fluid. Blood components refer to the physical separation of the collected whole blood into relatively single blood components with high purity and good efficacy, such as red blood cells, platelets, plasma, etc. Various blood components are collectively referred to as component blood.

[0003] The purpose of clinical blood transfusion is to maximize the therapeutic effects of blood. Component transfusion is an important milestone in the development of modern transfusion medicine. First, the use of component transfusion can significantly improve the therapeutic effect, because the relatively single blood component has the advantages of high concentration, the clinical transfusion effect is definite, and it can reduce or prevent the occurrence of adverse reactions to transfusion; secondly, the preservation of blood components is targeted using different storage conditions, which can optimize the preservation quality of blood components and maximize the preservation time. Therefore, it can not only ensure the therapeutic effect of clinical transfusion, but also maximize blood conservation and protect blood resources. Thirdly, the subsequent processing of blood components can further improve their quality, reduce the risk of transfusion-transmitted diseases, and make transfusion therapy safer.

[0004] Chinese patent CN105498010B discloses a fully automatic whole blood collection and separation device and method. This technical solution solves the problem of the existing method requiring multiple positions (the above-mentioned blood collection room and component room), multiple steps (blood collection, separation), and multiple equipment (blood collection scale, centrifuge, slurry separation clamp, etc.) by realizing the automation of whole blood collection and separation. It reduces the process of transporting whole blood from the blood collection room to the component room for separation, which is beneficial to improving the quality of blood components and facilitating the preparation of fresh frozen plasma and cryoprecipitate;

[0005] Chinese patent CN204582094U discloses a fully automatic whole blood collection and preparation machine with a matching disposable device. This technical solution solves the problems of time-consuming, labor-intensive, multi-site, and high cost in the existing whole blood collection and separation preparation process. It ensures that fresh plasma is separated from the whole blood at the fastest speed after it leaves the body, and is frozen in time to reduce the decay rate of unstable factors in the plasma as much as possible, improve the quality of the plasma, and provide conditions for preparing cold precipitation.

[0006] In the above technical solution, although it can automatically collect and separate whole blood in design, it is only limited to the collection and separation of whole blood and does not involve the subsequent preparation process of blood, resulting in a low degree of automation and inconvenient use of whole blood collection and separation. Summary of the Invention

[0007] The present utility model provides a fully automatic whole blood collection and preparation machine with a supporting disposable device, aiming to solve the problem that although it can automatically collect and separate whole blood in the current design process, it is only limited to the collection and separation of whole blood and does not involve the subsequent preparation process of blood. At the same time, a whole blood pipeline for blood matching is provided in the device of the present utility model to avoid the situation of transfusing red blood cells with inconsistent blood types that may occur clinically.

[0008] The present utility model is realized as follows. A fully automatic whole blood collection and preparation machine with a supporting disposable device includes a fully automatic whole blood collection and preparation machine and a disposable collection and preparation device. The fully automatic whole blood collection and preparation machine includes a control system, a pipeline light sensor, a pipeline pressure monitor, a weighing device, a pipeline air bubble monitor, a centrifuge system, valves, an air pressure cuff, and a peristaltic pump. The disposable collection and preparation device is detachably connected to the fully automatic whole blood collection and preparation machine, and each component of the disposable collection and preparation device is correspondingly arranged with the control system, the pipeline light sensor, the pipeline pressure monitor, the weighing device, the pipeline air bubble monitor, the centrifuge system, the valves, the air pressure of the air pressure cuff, and the peristaltic pump. The output end of the control system is electrically connected to the input ends of the centrifuge system, the valves, the air pressure of the air pressure cuff, and the peristaltic pump. The output ends of the pipeline light sensor, the pipeline pressure monitor, the weighing device, the pipeline air bubble monitor, the centrifuge system, and the air pressure of the air pressure cuff are all electrically connected to the input end of the control system, and the input end of the control system receives external input information.

[0009] Preferably, the control system includes programming, mode selection, start control, and stop control.

[0010] Preferably, the fully automatic whole blood collection and preparation machine further includes a chassis, which is composed of a housing, an operation interface, an air pressure cuff interface, and a consumable fixing pin.

[0011] Preferably, the fully automatic whole blood collection and preparation machine further includes a barcode reader and a storage system, and the storage system is composed of an information storage medium and an information output interface.

[0012] Preferably, the disposable collection and preparation device includes a donor line, a preservative solution line, a whole blood line, a centrifuge cup, and a plasma line. One end of the donor line is connected to a blood collection needle, and the middle section of the donor line is connected to a sample collection bag through a tee. One end of the preservative solution line is respectively connected to a blood preservative solution puncture device, a red blood cell additive puncture device, and a washing solution puncture device. The other end of the donor line and the other end of the preservative solution line are connected in parallel to the whole blood line. The middle section of the preservative solution line is successively connected in series to a preservative solution drip chamber, a bacteria filter, and a preservative solution pump line. The other end of the whole blood line is connected to the inlet of the centrifuge cup. The middle section of the whole blood line is successively connected through a tee to a whole blood line for blood matching, a whole blood line pressure monitor connector, and one end of the red blood cell line, and then connected in series to a whole blood pump line. The other end of the whole blood line for blood matching is connected to a leukocyte-reduced suspension red blood cell bag or a suspension red blood cell bag. The other end of the red blood cell line is respectively connected to a suspension red blood cell bag and a red blood cell temporary storage bag. The suspension red blood cell bag is connected to a leukocyte-reduced suspension red blood cell bag through a leukocyte filter and a leukocyte filter bypass. One end of the plasma line is connected to the outlet of the centrifuge cup, and the other end of the plasma line is connected to a plasma bag. The middle section of the plasma line is successively connected through a tee to a plasma line pressure monitor connector, a buffy coat bag, and an exhaust waste liquid bag. The plasma bag is connected to a cryoprecipitate standby bag.

[0013] Preferably, the valves include a blood preservative solution line valve, a whole blood line valve, a red blood cell temporary storage bag line valve, a suspension red blood cell line valve, a plasma line valve, a buffy coat line valve, and an exhaust waste liquid pipe valve.

[0014] Preferably, the centrifuge system is composed of a centrifuge chamber, a centrifuge chamber lid, and a centrifuge drive and rotation device. A centrifuge cup interface detector and a leak monitor are provided on the centrifuge chamber wall. The peristaltic pumps include a whole blood pump and a blood preservative solution pump.

[0015] Compared with the prior art processes and designs, the beneficial effects of the present utility model are as follows: The fully automatic whole blood collection and preparation machine of the present utility model is equipped with a supporting disposable device. In the present utility model, collection, separation, and preparation are carried out simultaneously. Plasma and red blood cells are separated from whole blood, and buffy coat can also be separated at the same time. Not only can red blood cells be separated from whole blood, but washed red blood cells can be further prepared on this device, and blood products that can be directly sent to the clinic can be prepared, with a long storage time. It can avoid the need for personnel in the blood component preparation department of the blood bank to work overtime at night and improve the blood emergency supply capacity. The prepared single-donor fresh liquid plasma can be immediately frozen into single-donor fresh frozen plasma. As the starting blood, single-donor fresh frozen plasma can be further prepared into cryoprecipitate coagulation factors and cryoprecipitate-depleted plasma. The quality of the prepared cryoprecipitate coagulation factors is better than that of the cryoprecipitate coagulation factors prepared from fresh frozen plasma used as the starting blood by the current blood component preparation department of the blood bank. Buffy coat, as the starting blood, can be further prepared into concentrated platelets, granulocytes, lymphocytes, etc. In the device, measures are added on how to connect the red blood cell series products sent to the clinic with the whole blood pipeline for blood matching, so as to avoid the situation where red blood cells with inconsistent blood types may be transfused clinically. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic structural diagram of the disposable collection and preparation device of the present utility model;

[0017] Figure 2 is a principle block diagram of the fully automatic whole blood collection and preparation machine of the present utility model;

[0018] In the figure: 1, blood collection needle; 2, donor pipeline; 3, sample retention bag; 4, blood preservative puncture device; 5, red blood cell additive puncture device; 6, washing liquid puncture device; 7, preservative pipeline; 8, bacteria filter; 9, preservative pump circuit; 10, whole blood pipeline; 11, whole blood pipeline for blood matching; 12, whole blood pipeline pressure monitor connector; 13, whole blood pump circuit; 14, centrifuge cup; 15, red blood cell pipeline; 16, suspended red blood cell bag; 17, white blood cell filter; 18, white blood cell filter bypass; 19, leukocyte-depleted suspended red blood cell bag; 20, red blood cell temporary storage bag; 21, plasma pipeline; 22, plasma pipeline pressure monitor connector; 23, plasma bag; 24, cryoprecipitate standby bag; 25, buffy coat bag; 26, exhaust waste liquid bag; 27, control system; 28, pipeline light sensor; 29, pipeline pressure monitor; 30, weighing device; 31, pipeline air bubble monitor; 32, centrifuge system; 33, valve; 34, pneumatic cuff; 35, peristaltic pump; 36, preservative drip kettle. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] In order to make the objectives, technical solutions and advantages of the present utility model more clear and understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0020] Please refer to Figure 1 and Figure 2 , the present utility model provides a technical solution: a fully automatic whole blood collection and preparation machine with a supporting disposable device, including a fully automatic whole blood collection and preparation machine and a disposable collection and preparation device. The fully automatic whole blood collection and preparation machine includes a control system 27, a pipeline light sensor 28, a pipeline pressure monitor 29, a weighing device 30, a pipeline air bubble monitor 31, a centrifuge system 32, a valve 33, an air pressure cuff 34 and a peristaltic pump 35. The disposable collection and preparation device is detachably connected to the fully automatic whole blood collection and preparation machine, and each component of the disposable collection and preparation device is respectively arranged corresponding to the control system 27, the pipeline light sensor 28, the pipeline pressure monitor 29, the weighing device 30, the pipeline air bubble monitor 31, the centrifuge system 32, the valve 33, the air pressure of the air pressure cuff 34 and the peristaltic pump 35. The output end of the control system 27 is electrically connected to the input ends of the centrifuge system 32, the valve 33, the air pressure of the air pressure cuff 34 and the peristaltic pump 35. The output ends of the pipeline light sensor 28, the pipeline pressure monitor 29, the weighing device 30, the pipeline air bubble monitor 31, the centrifuge system 32 and the air pressure of the air pressure cuff 34 are all electrically connected to the input end of the control system 27, and the input end of the control system 27 receives external input information.

[0021] Further, the control system 27 includes programming, mode selection, start control and stop control.

[0022] Further, the fully automatic whole blood collection and preparation machine further includes a chassis, which is composed of a housing, an operation interface, a pressure cuff interface and a consumable fixing pin.

[0023] Further, the fully automatic whole blood collection and preparation machine further includes a barcode reader and a storage system, and the storage system is composed of an information storage medium and an information output interface.

[0024] Further, the valve 33 includes a blood preservative solution pipeline valve, a whole blood pipeline valve, a red blood cell temporary storage bag pipeline valve, a suspended red blood cell pipeline valve, a plasma pipeline valve, a buffy coat pipeline valve and an exhaust waste liquid pipeline valve.

[0025] Further, the centrifuge system 32 includes a centrifuge chamber, a centrifuge chamber lid and a centrifuge drive rotating device. A centrifuge cup interface detector and a leak monitor are also provided on the centrifuge chamber wall. The peristaltic pump 35 includes a whole blood pump and a blood preservative solution pump.

[0026] After selecting the mode and starting, the fully automatic whole blood collection and preparation machine control system 27 is instructed by the pipeline light sensor 28, pipeline air bubble monitor 31, pipeline pressure monitor 29, weighing device 30, etc., to control the pressure in the upper arm cuff of the blood donor being collected, control the opening and closing of the blood preservation solution pipeline valve, whole blood pipeline valve, red blood cell temporary storage bag pipeline valve, suspended red blood cell pipeline valve, plasma pipeline valve, buffy coat pipeline valve, and exhaust waste liquid pipeline valve, etc., control the clockwise or counterclockwise rotation and stop of the whole blood pump and blood preservation solution pump; control the start and stop of the centrifuge.

[0027] The pumps include a whole blood pump and a blood preservation solution pump. The pipeline light sensor 28 senses the change in the turbidity of the plasma pipeline 21. The pipeline air bubble monitor 31 senses the air in the preservation solution pipeline 7 and the whole blood pipeline 10. The weighing device 30 measures the weight of the plasma.

[0028] Furthermore, the disposable collection and preparation device includes a blood donor pipeline 2, a preservation solution pipeline 7, a whole blood pipeline 10, a centrifuge cup 14, and a plasma pipeline 21. One end of the blood donor pipeline 2 is connected with a blood collection needle 1. The middle section of the blood donor pipeline 2 is connected with a sample collection bag 3 through a three-way connection. One end of the preservation solution pipeline 7 is respectively connected with a blood preservation solution puncture device 4, a red blood cell additive puncture device 5, and a washing solution puncture device 6. The other end of the blood donor pipeline 2 and the other end of the preservation solution pipeline 7 are connected in parallel to the whole blood pipeline 10. The middle section of the preservation solution pipeline 7 is successively connected in series with a preservation solution drip chamber 36, a bacteria filter 8, and a preservation solution pump path 9. The other end of the whole blood pipeline 10 is connected to the inlet of the centrifuge cup 14. The middle section of the whole blood pipeline 10 is successively connected through a three-way connection with a whole blood pipeline for blood matching 11, a whole blood pipeline pressure monitor connector 12, and one end of a red blood cell pipeline 15, and then connected in series with a whole blood pump path 13. The other end of the whole blood pipeline for blood matching 11 is connected to a leukocyte-reduced suspended red blood cell bag 19 or a suspended red blood cell bag 16. The other end of the red blood cell pipeline 15 is respectively connected to the suspended red blood cell bag 16 and a red blood cell temporary storage bag 20. The suspended red blood cell bag 16 is connected to the leukocyte-reduced suspended red blood cell bag 19 through a leukocyte filter 17 and a leukocyte filter bypass 18. One end of the plasma pipeline 21 is connected to the outlet of the centrifuge cup 14. The other end of the plasma pipeline 21 is connected to a plasma bag 23. The middle section of the plasma pipeline 21 is successively connected through a three-way connection with a plasma pipeline pressure monitor connector 22, a buffy coat bag 25, and an exhaust waste liquid bag 26. The plasma bag 23 is connected to a cryoprecipitate standby bag 24.

[0029] The preparation method of a fully automatic whole blood collection and preparation machine with a supporting disposable device includes the following steps:

[0030] S1. Collect the peripheral venous blood of the blood donor through the blood collection needle 1, mix it with the blood preservation solution in the blood donor pipeline 2 and the preservation solution pipeline 7 by the rotational speed coordination of the whole blood pump and the blood preservation solution pump, mix it into whole blood in a certain proportion, and input it into the centrifuge cup 14 through the whole blood pipeline 10 for centrifugation;

[0031] S2. The apheresis fresh liquid plasma and buffy coat are separated and sequentially collected into a plasma bag 23 and a buffy coat bag 25 through a plasma pipeline 21 respectively. The red blood cells in the centrifuge cup 14 are reversely collected into a suspended red blood cell bag 16 or a red blood cell temporary storage bag 20 through a whole blood pipeline 10 and a red blood cell pipeline 15, thus completing one cycle.

[0032] S3. When the blood collection volume reaches 200 mL, 300 mL or 400 mL set as the target, blood collection is stopped, the blood preservative solution pump stops running, and centrifugation continues. The suspended red blood cell pipeline valve or the red blood cell temporary storage bag pipeline valve is opened, and the red blood cells are input into the centrifuge cup 14 through the red blood cell pipeline 15 and the whole blood pipeline 10, and plasma and buffy coat are continuously collected in sequence until one cycle is completed.

[0033] S4. The flow stop clamp on the whole blood pipeline for blood matching is opened, and the whole blood in the whole blood pipeline is transported to the whole blood pipeline for blood matching through the peristalsis of the blood preservative solution pump. Heat sealing is performed and the connection between the whole blood pipeline for blood matching and the whole blood pipeline is severed, and the whole blood pipeline for blood matching is reserved and connected to the leukocyte-reduced suspended red blood cell bag or the suspended red blood cell bag.

[0034] Furthermore, according to clinical needs, for the red blood cells, on this whole blood collection and preparation machine, the flow stop clamps of the blood preservative solution and the red blood cell additive solution are closed, the flow stop clamp of the red blood cell washing solution is opened, and through a preservative solution pipeline 7, a whole blood pipeline 10 and a red blood cell pipeline 15, the washing solution, generally 0.9% NaCl, is added to the red blood cell temporary storage bag 20 and mixed. The red blood cells added with the washing solution are input into the centrifuge cup 14 through the red blood cell pipeline 15 and the whole blood pipeline 10, and the washing solution is removed to an exhaust waste liquid bag 26 through centrifugation. The washed red blood cells are reversely collected into a suspended red blood cell bag 16 through the whole blood pipeline 10 and the red blood cell pipeline 15. Thus, the red blood cells are transferred between the red blood cell temporary storage bag 20 and the suspended red blood cell bag 16. After 3 times of centrifugal washing, a red blood cell additive solution is added to prepare washed and preserved red blood cells, which are stored in the suspended red blood cell bag 16; the washed and preserved red blood cells are continuously prepared into leukocyte-reduced washed and preserved red blood cells through a leukocyte filter 17 and stored in a leukocyte-reduced suspended red blood cell bag 19. The above-prepared various red blood cells are stored in an environment of 2 - 6°C.

[0035] Select the collection and preparation mode. After the disposable device is installed in place, when the blood collection needle 1 successfully punctures the elbow vein of the blood donor, turn on the whole blood collection and preparation machine, and let the initially flowing blood enter the sample collection bag 3; the anticoagulant pump rotates clockwise, driving the anticoagulant to flow in the anticoagulant pump line 9 and mixing with the peripheral venous blood of the blood donor in the blood donor line 2 at a certain ratio to form whole blood, which flows into the whole blood line 10. The whole blood pump rotates clockwise, driving the whole blood to flow in the whole blood pump line 13, entering the centrifuge cup 14 through the inlet of the centrifuge cup 14. At the same time, the centrifuge drives the centrifuge cup 14 to start rotating for centrifugal separation. First, fresh liquid plasma is separated and enters the plasma bag 23 through the outlet of the centrifuge cup 14; when the interface detector on the inner wall of the centrifuge chamber and the plasma line detector detect that the flowing liquid in the line changes from plasma to buffy coat, the buffy coat is separated and continues to enter the buffy coat bag 25; when the interface detector on the inner wall of the centrifuge chamber and the plasma line detector detect that the flowing liquid in the line changes from buffy coat to red blood cells, stop blood collection, stop the anticoagulant pump and the whole blood pump, and apply the centrifuge brake; after the centrifuge stops, the whole blood pump rotates counterclockwise, and the red blood cells in the centrifuge are collected into the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20 through the whole blood line 10 and the red blood cell line 15. The anticoagulant line valve, the whole blood line valve, the plasma line valve, the buffy coat line valve, the red blood cell temporary storage bag line valve, and the suspended red blood cell line valve cooperate with each other to ensure the correct blood flow in the line. When the air bubble detector in the whole blood line detects that all the red blood cells have entered the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20, the whole blood pump stops rotating counterclockwise.

[0036] According to the above process, perform the second cycle of blood collection, and so on for 2 - 4 cycles of blood collection. When the target blood collection volume of 200 mL, 300 mL, or 400 mL is reached, stop blood collection, close the stop clamp on the blood donor line 2, and pull out the needle; stop the anticoagulant pump, and the whole blood pump continues to rotate clockwise. At the same time, close the whole blood line valve, and open the suspended red blood cell line valve or the red blood cell temporary storage bag line valve. The red blood cells in the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20 are replenished into the centrifuge cup 14 to complete the last blood collection and preparation process; after the air bubble detector in the whole blood line detects that all the red blood cells have entered the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20, stop the whole blood pump, close the suspended red blood cell line valve and the red blood cell temporary storage bag line valve, open the whole blood line valve and the stop clamp of the whole blood line for blood matching, the anticoagulant pump rotates clockwise, and the remaining whole blood in the whole blood line 10 is transported to the whole blood line 11 for blood matching. Heat seal and disconnect the connection between the whole blood line 11 for blood matching and the whole blood line 10, and keep the whole blood line 11 for blood matching connected to the leukocyte-reduced suspended red blood cell bag 19 or the suspended red blood cell bag 16.

[0037] If washed red blood cells are not prepared, the red blood cell temporary storage bag 20 and the exhaust waste liquid bag 26 can be removed from the device; the prepared red blood cells directly enter the suspended red blood cell bag 16; the red blood cell additive puncture device 5 is connected to the red blood cell additive, the maintenance fluid pump is started to rotate clockwise, and a certain proportion of red blood cell additive is added to the suspended red blood cell bag 16, and after mixing, suspended red blood cells or concentrated red blood cells are prepared; the suspended red blood cells or concentrated red blood cells are continuously prepared into leukocyte-reduced suspended red blood cells or leukocyte-reduced concentrated red blood cells through the leukocyte filter 17 and stored in the leukocyte-reduced suspended red blood cell bag 19.

[0038] If washed red blood cells are to be prepared, after blood collection stops, the washing liquid puncture device 6 is connected to the washing liquid, generally 0.9% NaCl, the maintenance fluid pump is started to rotate clockwise, and a certain amount of washing liquid is added to the red blood cell temporary storage bag 20 through the maintenance fluid pipeline 7, the whole blood pipeline 10 and the red blood cell pipeline 15 and mixed with the red blood cells. Then the maintenance fluid pump stops running, the whole blood pump rotates clockwise, and at the same time the whole blood pipeline valve is closed. The red blood cells added with the washing liquid in the red blood cell temporary storage bag 20 are input into the centrifuge cup 14 through the red blood cell pipeline 15 and the whole blood pipeline 10. Through centrifugation, the washing liquid is separated and collected in the exhaust waste liquid bag 26 through the outlet of the centrifuge cup 14 and the plasma pipeline 21. When the plasma pipeline detector detects that the flowing liquid in the pipeline becomes red blood cells, the whole blood pump stops running and the centrifuge brakes; after the centrifuge stops, the whole blood pump rotates counterclockwise, and the washed red blood cells in the centrifuge cup are collected in the suspended red blood cell bag 16 in the reverse direction through the whole blood pipeline 10 and the red blood cell pipeline 15. The above process is continued to wash the red blood cells in the red blood cell temporary storage bag 20 once, and all the washed red blood cells are collected in the suspended red blood cell bag 16. In this way, the red blood cells are emptied between the red blood cell temporary storage bag 20 and the suspended red blood cell bag 16, and after 3 times of centrifugal washing, the red blood cell additive puncture device 5 is connected to the red blood cell additive, the maintenance fluid pump is started to rotate clockwise, and a certain proportion of red blood cell additive is input into the suspended red blood cell bag 16, and after mixing, washed and preserved red blood cells are prepared; the washed and preserved red blood cells are continuously prepared into leukocyte-reduced washed and preserved red blood cells through the leukocyte filter 17 and stored in the leukocyte-reduced suspended red blood cell bag 19.

[0039] The prepared buffy coat is stored in an environment of 18 - 25 °C and can be further prepared into concentrated platelets, granulocytes and lymphocytes. Among them, multiple bags of concentrated platelets can form mixed concentrated platelets.

[0040] The prepared apheresis fresh liquid plasma is frozen within 6 - 8 hours and becomes apheresis fresh frozen plasma after freezing, and can be further prepared into cryoprecipitate and cryoprecipitate-depleted plasma.

[0041] The mode control in the control system 27 includes: Mode 1 for preparing plasma and red blood cells, Mode 2 for preparing plasma, red blood cells and buffy coat, and Mode 3 for washing red blood cells:

[0042] Mode 1: The collected blood is prepared into suspended red blood cells and fresh liquid plasma, and the corresponding disposable device can remove the red blood cell temporary storage bag 20, buffy coat bag 25, and exhaust waste liquid bag 26; the suspended red blood cells or concentrated red blood cells are not further prepared into leukocyte-reduced suspended red blood cells or leukocyte-reduced concentrated red blood cells, and the leukocyte filter 17, leukocyte filter bypass 18, and leukocyte-reduced suspended red blood cell bag 19 can be removed, and the whole blood circuit 11 for blood matching is directly connected to the suspended red blood cell bag 16; the plasma is not further prepared into cryoprecipitate coagulation factor, and the cryoprecipitate spare bag 24 can be removed.

[0043] Mode 2: The collected blood is prepared into suspended red blood cells, fresh liquid plasma, and buffy coat, and the corresponding disposable device can remove the red blood cell temporary storage bag 20 and exhaust waste liquid bag 26; the suspended red blood cells or concentrated red blood cells are not further prepared into leukocyte-reduced suspended red blood cells or leukocyte-reduced concentrated red blood cells, and the leukocyte filter 17, leukocyte filter bypass 18, and leukocyte-reduced suspended red blood cell bag 19 can be removed, and the whole blood circuit 11 for blood matching is directly connected to the suspended red blood cell bag 16; the plasma is not further prepared into cryoprecipitate coagulation factor, and the cryoprecipitate spare bag 24 can be removed.

[0044] Mode 3: The collected red blood cells need to be further prepared into washed and preserved red blood cells, and the corresponding disposable device should retain the red blood cell temporary storage bag 20 and exhaust waste liquid bag 26; the washed and preserved red blood cells are not further prepared into leukocyte-reduced washed and preserved red blood cells, and the leukocyte filter 17, leukocyte filter bypass 18, and leukocyte-reduced suspended red blood cell bag 19 can be removed, and the whole blood circuit 11 for blood matching is directly connected to the suspended red blood cell bag 16; the plasma is not further prepared into cryoprecipitate coagulation factor, and the cryoprecipitate spare bag 24 can be removed.

[0045] Working principle and usage process of the utility model: After the utility model is installed, the peripheral venous blood of the blood donor is collected and mixed with a certain proportion of blood preservative solution to form whole blood. The whole blood is centrifuged and separated, and the plasma is collected in the plasma bag 23, and then the buffy coat is collected in the buffy coat bag 25. The collection of whole blood is stopped, and the red blood cells are collected in the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20; the above steps 2-4 are repeated until the set collection volume is reached, and blood collection is stopped. The red blood cells in the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20 are supplemented to the centrifuge cup 14, and the plasma is continuously collected in the plasma bag 23, and then the buffy coat is collected in the buffy coat bag 25, and the red blood cells are collected in the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20, and the red blood cell additive solution is added to the suspended red blood cell bag 16 to mix with the red blood cells; the peripheral venous blood of the blood donor is collected and mixed with the blood preservative solution to form whole blood. The blood donor's elbow vein is punctured by the blood collection needle 1 through the pipeline. Under the clockwise peristalsis of the whole blood pump, and the blood preservative solution bag interface is punctured by the blood preservative solution puncture device 4, through the pipeline connecting the bacteria filter 8, under the clockwise peristalsis of the blood preservative solution pump, according to a certain proportion, the peripheral venous blood of the blood donor and the blood preservative solution are mixed to form whole blood through the three-way pipe. The whole blood pump peristalsis clockwise transports the whole blood through the inlet of the centrifuge cup 14 into the centrifuge cup 14 installed in the centrifugation chamber. After high-speed centrifugation, it is separated into the upper plasma, the middle buffy coat and the lower red blood cells. The separated plasma is collected in the plasma bag 23 from the outlet of the centrifuge cup 14. At the same time, the buffy coat pipeline valve and the exhaust waste liquid pipeline valve are closed, and the separation continues. When the interface detector on the inner wall of the centrifugation chamber and the plasma pipeline light sensor 28 sense the buffy coat layer, the plasma pipeline valve is closed, the buffy coat pipeline valve is opened, and the buffy coat is continuously collected in the buffy coat bag 25. When the interface detector on the inner wall of the centrifugation chamber and the plasma pipeline light sensor 28 sense the red blood cell layer, blood collection is stopped, the blood preservative solution pump stops running, the whole blood pipeline valve is closed, centrifugation is stopped, the buffy coat pipeline valve is closed, the plasma pipeline valve is opened, the suspended red blood cell pipeline valve or the red blood cell temporary storage bag pipeline valve is opened, and the whole blood pump peristalsis counterclockwise, and the red blood cells in the centrifuge cup 14 are collected from the inlet of the centrifuge cup 14 through the whole blood pipeline 10 and the red blood cell pipeline 15 into the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20; at the same time, the air pressure of the air pressure cuff 34 is pressurized when the whole blood pump peristalsis clockwise, and the air pressure in the air pressure cuff 34 is released when the whole blood pump stops peristalsis or peristalsis counterclockwise. When the whole blood pipeline air bubble monitor 31 senses air, the whole blood pump stops peristalsis, the suspended red blood cell pipeline valve or the red blood cell temporary storage bag pipeline valve is closed, and the collection of red blood cells is stopped; the above steps are repeated to start the second process, and so on. Generally, 3-4 cycles are required to collect 400 mL of whole blood. When in the last process, when the blood collection volume reaches the target set of 200 mL, 300 mL or 400 mL, blood collection is stopped, the blood preservative solution pump stops running, centrifugation continues, the suspended red blood cell pipeline valve or the red blood cell temporary storage bag pipeline valve is opened, and the red blood cells in the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20 are input into the centrifuge cup 14, and the plasma and the buffy coat are continuously collected in turn;When the interface detector to the inner wall of the centrifugation chamber and the plasma pipeline optical sensor 28 sense the red blood cell layer, stop the input of red blood cells into the centrifuge cup 14, stop centrifugation, close the buffy coat pipeline valve, open the plasma pipeline valve, the whole blood pump peristalsis counterclockwise, and collect the red blood cells in the centrifuge cup 14 from the inlet of the centrifuge cup 14 through the whole blood pipeline 10 and the red blood cell pipeline 15 into the suspended red blood cell bag 16 or the red blood cell temporary storage bag 20. Loosen the pressure cuff, close the flow stop clamp on the donor pipeline 2 and then pull out the needle; open the flow stop clamp on the whole blood pipeline 11 for blood matching, the blood preservative solution pump peristalsis clockwise, and transport the whole blood in the whole blood pipeline 10 into the whole blood pipeline 11 for blood matching. Heat seal and disconnect the connection between the whole blood pipeline 11 for blood matching and the whole blood pipeline 10, and keep the whole blood pipeline 11 for blood matching connected to the leukocyte-reduced suspended red blood cell bag 19 or the suspended red blood cell bag 16; puncture the washing solution interface with the washing solution puncture device 6, and through the pipeline connected to the bacteria filter, under the clockwise peristalsis of the blood preservative solution pump, mix with the red blood cells in the red blood cell temporary storage bag 20 and transport them into the centrifuge cup 14 through the inlet of the centrifuge cup 14. After dilution, centrifugation, and transfer, wash a total of 3 times, collect the waste liquid after washing into the exhaust waste liquid bag 26, input the washed red blood cells into the suspended red blood cell bag 16, add a certain proportion of red blood cell additive solution, and prepare washed preserved red blood cells. Then, further remove white blood cells through a leukocyte filter to prepare leukocyte-reduced washed preserved red blood cells. In the above steps, it is also possible to only collect plasma and red blood cells, not collect the buffy coat, and collect the buffy coat into the red blood cells together. In the above steps, it is also possible not to prepare washed preserved red blood cells. During the preparation process, for red blood cells that do not need to be washed, they can be directly input into the suspended red blood cell bag 16 during the preparation process, and then add a certain proportion of red blood cell additive solution to prepare suspended red blood cells or concentrated red blood cells. Further remove white blood cells through the leukocyte filter 17 to prepare leukocyte-reduced suspended red blood cells and leukocyte-reduced concentrated red blood cells. The plasma in the plasma bag 23 is frozen within 6 to 8 hours to prepare apheresis fresh frozen plasma; apheresis fresh frozen plasma as the starting blood can be further prepared into cryoprecipitate coagulation factors and cryoprecipitate-depleted plasma, etc. The buffy coat as the starting blood can be further prepared into concentrated platelets, granulocytes, and lymphocytes.

[0046] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A fully automatic whole blood collection and preparation machine with a matching disposable device, comprising a fully automatic whole blood collection and preparation machine and a matching disposable collection and preparation device, characterized in that: The fully automatic whole blood collection and preparation machine comprises a control system (27), a pipeline light sensor (28), a pipeline pressure monitor (29), a weighing device (30), a pipeline bubble monitor (31), a centrifuge system (32), a valve (33), an air pressure cuff (34) and a peristaltic pump (35); the disposable collection and preparation device is detachably connected to the fully automatic whole blood collection and preparation machine, and the components of the disposable collection and preparation device are respectively connected to the control system (27), the pipeline light sensor (28), the pipeline pressure monitor (29), the weighing device (30), the pipeline bubble monitor (31), the centrifuge system (32), the The valve (33), the air pressure of the air pressure cuff (34) and the peristaltic pump (35) are arranged correspondingly, the output end of the control system (27) is electrically connected to the centrifuge system (32), the valve (33), the air pressure of the air pressure cuff (34) and the input end of the peristaltic pump (35), respectively, the output ends of the pipeline light sensor (28), the pipeline pressure monitor (29), the weighing device (30), the pipeline bubble monitor (31), the centrifuge system (32) and the air pressure of the air pressure cuff (34) are all electrically connected to the input end of the control system (27), and the input end of the control system (27) receives external input information.

2. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The control system (27) includes programming, mode selection, start control and stop control.

3. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The fully automatic whole blood collection and preparation machine also includes a chassis, which is composed of a shell, an operation interface, a pressure cuff interface and consumable fixing pins.

4. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The fully automatic whole blood collection and preparation machine also includes a barcode reader and a storage system, and the storage system is composed of an information storage medium and an information output interface.

5. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The disposable collection and preparation device comprises a blood donor pipeline (2), a maintenance fluid pipeline (7), a whole blood vessel pipeline (10), a centrifuge cup (14) and a plasma pipeline (21); one end of the blood donor pipeline (2) is connected to a blood collection needle (1); the middle section of the blood donor pipeline (2) is connected to a sample bag (3) through a three-way connection; one end of the maintenance fluid pipeline (7) is respectively connected to a blood maintenance fluid puncture device (4), a red blood cell additive fluid puncture device (5), a washing fluid puncture device (6) and a plasma plasma puncture device (7). The other end of the blood donor pipeline (2) and the other end of the maintenance liquid pipeline (7) are connected in parallel to a whole blood vessel pipeline (10); the middle section of the maintenance liquid pipeline (7) is connected in series with a maintenance liquid drip pot (36), a bacterial filter (8) and a maintenance liquid pump circuit (9); the other end of the whole blood vessel pipeline (10) is connected to the inlet of the centrifuge cup (14); the middle section of the whole blood vessel pipeline (10) is connected in series with a whole blood vessel pipeline (11) for blood matching through a three-way connection. The whole blood vessel pressure monitor connector (12) and one end of the red blood cell pipeline (15) are connected in series to the whole blood pump circuit (13). The other end of the whole blood vessel circuit (11) for blood matching is connected to a leukocyte-removed suspended red blood cell bag (19) or a suspended red blood cell bag (16). The other end of the red blood cell pipeline (15) is respectively connected to a suspended red blood cell bag (16) and a red blood cell temporary storage bag (20). The suspended red blood cell bag (16) is filtered through a leukocyte filter (17) and a leukocyte filter (20). The bypass (18) is connected to the leukocyte-removed suspended red blood cell bag (19), one end of the plasma pipeline (21) is connected to the outlet of the centrifuge cup (14), the other end of the plasma pipeline (21) is connected to a plasma bag (23), the middle section of the plasma pipeline (21) is connected in sequence to a plasma pipeline pressure monitor connector (22), a white film bag (25) and an exhaust waste liquid bag (26) through a three-way connection, and the plasma bag (23) is connected to a cold precipitation standby bag (24).

6. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The valve (33) comprises a blood maintenance liquid pipeline valve, a full blood vessel pipeline valve, a red blood cell temporary storage bag pipeline valve, a suspended red blood cell pipeline valve, a plasma pipeline valve, a white membrane pipeline valve and an exhaust waste liquid pipeline valve.

7. The fully automatic whole blood collection and preparation machine with a matching disposable device as claimed in claim 1, characterized in that: The centrifuge system (32) is composed of a centrifuge chamber, a centrifuge chamber cover and a centrifuge drive rotating device. The centrifuge chamber wall is provided with a centrifuge cup interface detector and a liquid leakage monitor. The peristaltic pump (35) includes a whole blood pump and a blood maintenance liquid pump.

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