Microbial liquid culture transfer device and system for producing lycopene

Abstract

The utility model discloses a microbial liquid culture transfer device for producing lycopene and a system thereof. The microbial liquid culture transfer device comprises a tank body 11, a movable bracket 3, a stirring device 41, a conveying pipeline 21 and a pressure balance pipe 22, the movable support 3 is installed at the bottom of the tank body 11, the stirring device 41 is located at the bottom of the interior of the tank body 11, the conveying pipeline 21 penetrates through the top of the tank body and extends to the bottom of the interior of the tank body 11, and the pressure balance pipe 22 penetrates through the top of the tank body 11 but does not continue to extend to the bottom of the tank body 11. Positive bacteria and negative bacteria of blakeslea trispora located in different seed tanks can be transferred into the transfer device 1 to be mixed, the transfer device 1 is moved to the fermentation tank, and mixed seed liquid in the transfer device 1 is transferred into the fermentation tank. Therefore, the problems that the seed tank is far away from the fermentation tank and is difficult to transfer are solved. The transfer difficulty is reduced, the transfer time is shortened, the production efficiency is improved, and the risk that the bacterial liquid is polluted can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of fermentation devices, and particularly relates to a device and a system for transferring a microbial liquid culture for producing lycopene. Background Art

[0002] Lycopene is an unsaturated olefin compound, which widely exists in tomatoes, tomato products, and fruits such as watermelons and grapefruits. It is the main pigment in ripe tomatoes and is also one of the common carotenoids. At present, lycopene has not only been widely used as a natural pigment, but also has been increasingly applied to functional foods, pharmaceuticals, and cosmetics. The main physiological functions and effects of lycopene include: enhancing the body's oxidative stress ability and anti-inflammatory effect, protecting the cardiovascular and cerebrovascular system, reducing the damage of the skin by radiation or ultraviolet rays (UV), and enhancing immunity.

[0003] Blakeslea trispora is a commonly used strain for producing carotenoids in industry. It is a heterothallic fungus, including two unisexual strains, the positive strain and the negative strain. Its mycelium grows rapidly and can grow into thick long hyphae. Usually, in the seed fermentation stage, the positive and negative strains are fermented separately, and then inoculated into the same fermentation tank in different volume ratios for combined fermentation to produce lycopene.

[0004] Since the positive and negative strains are prepared separately and have a large volume difference, different requirements for the configuration of the seed tank and the fermentation tank are caused. In the actual production site, the suitable seed tank and fermentation tank are often far apart, and the inoculation and transfer processes need to be operated over a long distance. At this time, there are still many risks in the transfer and inoculation processes. Therefore, in industrial production, a transfer container that can reduce the pollution risk is urgently needed. Summary of the Invention

[0005] In order to solve the above problems, the utility model provides a device for transferring a microbial liquid culture for producing lycopene,

[0006] which includes a tank body, a movable support, a stirring device, a conveying pipeline, and a pressure balance pipe;

[0007] The movable support is installed at the bottom of the tank body, the stirring device is located at the bottom inside the tank body, the conveying pipeline penetrates through the top of the tank body and extends to the bottom inside the tank body, and the pressure balance pipe penetrates through the top of the tank body but does not continue to extend towards the bottom of the tank body.

[0008] Preferably, control valves are provided on the conveying pipeline and the pressure balance pipe, and the control valves are located outside the tank body.

[0009] Preferably, a sterilization device is further included;

[0010] The sterilization device is located outside the tank body and is connected to the end of the pressure balance pipe.

[0011] Preferably, a microbial filtration device is further provided on the pressure balance pipe and is located between the tank body and the control valve.

[0012] Preferably, an observation window for observing the inside of the tank body is provided on the tank wall of the tank body.

[0013] Preferably, scales are provided on the observation window.

[0014] Preferably, the stirring device is connected to a stirring control device located outside the tank body through a connecting rod.

[0015] Preferably, the stirring device is a magnetic stirring device and is controlled by magnetism by a stirring control device located outside the tank body.

[0016] The present utility model further provides a microbial liquid culture transfer system for producing lycopene, including the microbial liquid culture transfer device and a hose described above;

[0017] The hose connects the culture device and the microbial liquid culture transfer device.

[0018] Preferably, a peristaltic pump is provided on the hose. Description of the Drawings

[0019] Figure 1 : Schematic diagram of an embodiment, microbial liquid culture transfer device;

[0020] Figure 2 : Schematic diagram of an embodiment, transfer of microbial liquid culture in the seed tank to the transfer device, the arrow indicates the conveying direction;

[0021] Figure 3 : Schematic diagram of an embodiment, transfer of microbial liquid culture in the transfer device to the fermentation tank, the arrow indicates the conveying direction.

[0022] Reference numerals: 1 - transfer device, 11 - tank body, 12 - observation window, 21 - conveying pipeline, 211 - control valve, 22 - pressure balance pipe, 221 - sterilization device, 222 - control valve, 223 - microbial filtration device, 3 - movable support, 41 - stirring device, 42 - stirring control device, 5 - hose, 6 - peristaltic pump, 7 - culture device. Detailed Embodiments

[0023] The experimental methods used in the following embodiments are all conventional methods unless otherwise specified.

[0024] Unless otherwise specified, the materials, reagents, etc. used in the following embodiments are all ordinary commercially available products and can be purchased on the market.

[0025] The present utility model will be further described below through embodiments, and these descriptions do not further limit the content of the present utility model. Those skilled in the art should understand that equivalent replacements or corresponding improvements made to the content of the present utility model still fall within the protection scope of the present utility model.

[0026] In one embodiment, as Figure 1 shown, a microbial liquid culture transfer device 1 for producing lycopene includes a tank body 11, a movable bracket 3, a stirring device 41, a conveying pipeline 21, and a pressure balance pipe 22; the movable bracket 3 is installed at the bottom of the tank body 11, the stirring device 41 is located at the bottom inside the tank body 11, the conveying pipeline 21 penetrates through the top of the tank body and extends to the bottom inside the tank body 11, and the pressure balance pipe 22 penetrates through the top of the tank body 11 but does not continue to extend towards the bottom of the tank body 11.

[0027] The positive and negative bacteria of Blakeslea trispora in different seed tanks can be transferred to the transfer device 1 for mixing, and the transfer device 1 is moved to the fermentation tank, and the mixed seed liquid in the transfer device 1 is transferred to the fermentation tank. Thus, the problem that the distance between the seed tank and the fermentation tank is far and it is not easy to transfer is overcome. It not only reduces the transfer difficulty, shortens the transfer time, and improves the production efficiency. It can also reduce the risk of the bacterial liquid being contaminated.

[0028] In a preferred embodiment, control valves 211(222) are provided on the conveying pipeline 21 and the pressure balance pipe 22, and the control valves 211(222) are located outside the tank body 11.

[0029] In a preferred embodiment, a sterilization device 221 is further included; the sterilization device 221 is located outside the tank body 11 and is connected to the end of the pressure balance pipe 22. A specific example of the sterilization device 221 is an alcohol lamp with a stainless steel cover, which performs high-temperature sterilization on the air flowing into the tank body 11.

[0030] In a preferred embodiment, a microbial filtration device 223 is further provided on the pressure balance pipe 22 and is located between the tank body 11 and the control valve 222.

[0031] In a preferred embodiment, an observation window 12 for observing the inside of the tank body is provided on the tank wall of the tank body 11.

[0032] In a preferred embodiment, the observation window 12 is provided with scales.

[0033] There is a scale on the observation window 12. The volume of the positive and negative strains transferred into the tank body 11 can be clearly judged, which is convenient for reference in the subsequent further transfer and fermentation in the fermentation tank.

[0034] In a preferred embodiment, the observation window 12 is a glass window.

[0035] In a preferred embodiment, the stirring device 41 is connected to a stirring control device 42 located outside the tank body through a connecting rod.

[0036] In a preferred embodiment, the stirring device 41 is a magnetic stirring device and is controlled by a magnetic force by the stirring control device 42 located outside the tank body 11.

[0037] The positive and negative strains can be quickly mixed through the stirring device 41. And for the convenience of cleaning and reducing the residue of the fermentation product, a magnetic stirring device can be used for stirring.

[0038] The present utility model also provides a microorganism liquid culture transfer system for producing lycopene, including the microorganism liquid culture transfer device 1 and a hose 5; the hose 5 connects the culture device 7 and the microorganism liquid culture transfer device 1.

[0039] In a preferred embodiment, a peristaltic pump 6 is provided on the hose.

[0040] In the implementation of a specific transfer system or transfer device 1, as Figure 2 shown, when the positive bacteria of Blakeslea trispora are about to complete fermentation in the seed tank (culture device 7), connect the delivery pipeline 21 of the transfer device 1 to the outlet pipeline at the bottom of the culture device 7 through the hose 5, open the control valve 211, close the control valve 222, open the steam pipeline valve of the culture device 7, and the steam enters the transfer device 1 through the outlet pipeline for sterilization.

[0041] After the sterilization is completed, close the steam pipeline valve. After the whole system cools down, light the alcohol lamp at the end of the transfer tank (sterilization device 221), open the control valve 222, put the hose 5 into the peristaltic pump 6, and the seed liquid of the culture device 7 enters the transfer device 1 through the outlet pipeline at the bottom under the push of the peristaltic pump 6. In order to avoid the strains from sinking to the bottom, the stirring control device 42 can be turned on. After the transfer is completed, close the control valve 211 and the control valve 222.

[0042] Subsequently, connect the transfer device 1 to the seed tank (culture device 7) for culturing negative bacteria. After the pipeline is sterilized, open the control valve 211 and the control valve 222 for filling. The flowing-in negative bacteria are mixed with the positive bacteria under the action of the stirring device 41. After the transfer is completed, close the control valve 211 and the control valve 222.

[0043] AsFigure 3 As shown, connect the conveying pipeline 21 of the transfer device 1 to the inlet pipeline at the top of the fermenter (cultivation device 7) through the hose 5. After the pipeline is sterilized and cooled, open the control valve 211 and the control valve 222. The seed liquid enters the cultivation device 7 under the push of the peristaltic pump 6.

Claims

1. A device for transferring liquid culture of microorganisms for producing lycopene, characterized in that: It includes a tank body, a movable bracket, a stirring device, a conveying pipeline, and a pressure balance pipe; The movable bracket is installed at the bottom of the tank body, the stirring device is located at the bottom of the tank body, the conveying pipeline passes through the top of the tank body and extends to the bottom of the tank body, and the pressure balance pipe passes through the top of the tank body but does not continue to extend to the bottom of the tank body.

2. The microbial liquid culture transfer device according to claim 1, characterized in that: The delivery pipeline and the pressure balance pipe are provided with control valves, and the control valves are located outside the tank body.

3. The microbial liquid culture transfer device according to claim 2, characterized in that: Also included is a sterilization device; The sterilization device is located outside the tank body and is connected to the end of the pressure balance pipe.

4. The microbial liquid culture transfer device according to claim 3, characterized in that: The pressure balance pipe is also provided with a microbial filtering device, and is located between the tank body and the control valve.

5. The microbial liquid culture transfer device according to any one of claims 1 to 4, characterized in that: An observation window for observing the inside of the tank body is arranged on the tank wall of the tank body.

6. The microbial liquid culture transfer device according to claim 5, characterized in that: The observation window is provided with scales.

7. The microbial liquid culture transfer device according to claim 6, characterized in that: The stirring device is connected to a stirring control device located outside the tank body through a connecting rod.

8. The microbial liquid culture transfer device according to claim 6, characterized in that: The stirring device is a magnetic stirring device, and is controlled by a stirring control device located outside the tank body through magnetic force.

9. A microbial liquid culture transfer system for producing lycopene, characterized in that: A device for transferring a microbial liquid culture and a hose according to any one of claims 1 to 8; The hose connects the culture device and the microorganism liquid culture transfer device.

10. The microbial liquid culture transfer system according to claim 9, characterized in that: The hose is provided with a peristaltic pump.

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