Cell collecting device convenient to use

Abstract

The utility model relates to the field of cell collecting devices, and discloses a cell collecting device convenient to use, which comprises a constant-temperature box and a storage test tube, a plurality of small culture dishes are arranged close to two sides in the constant-temperature box, bumps are fixedly arranged close to the center of two sides of the inner wall of each small culture dish in the plurality of small culture dishes, and the storage test tube is arranged in the constant-temperature box. Second filter plates are arranged at the positions, located at the upper ends of the two protruding blocks of each small culture dish, in the multiple small culture dishes, discharging blocks are fixedly arranged at the positions, close to the centers of the inner sides, of the inner walls of the multiple small culture dishes, a centrifugal machine is fixedly arranged at the upper end of the bottom of the constant-temperature box, and two test tube grooves are formed in a slope at the upper end of the centrifugal machine in a penetrating mode. According to the cell co-culture device disclosed by the utility model, the centrifugal machine and the culture dishes are integrated, so that cells can be collected by adjusting a proper temperature in the constant temperature box, centrifuged supernate and cells can be quickly and separately treated, and the cells and the supernate can be more accurately and separately placed in different small culture dishes as required for cell co-culture.

Description

Technical Field

[0001] The utility model relates to the field of cell collection devices, in particular to a cell collection device which is convenient to use. Background Technique

[0002] A cell collection device is a device used to separate and collect cells from biological samples. Since the extracted samples often contain substances other than the required cells, in order to only extract the cells for analysis and separate the cells from other substances, a cell collection device is needed.

[0003] The existing cell collection devices are usually inconvenient to operate. Usually, after each operation, equipment such as a pipette is needed to suck out the separated supernatant, and then the subsequent cells are processed. However, it cannot be guaranteed that the supernatant is completely sucked out. Excessive residual supernatant in the required cells will have an adverse effect on subsequent analysis. Especially in cell co-culture, one of the available methods of cell co-culture is to culture the supernatant of one type of cell and another type of cell in a culture dish, which requires a certain degree of precision in extraction.

[0004] Therefore, those skilled in the art have provided a cell collection device which is convenient to use to solve the problems raised in the above background technique. Summary of the Invention

[0005] The purpose of the utility model is to solve the disadvantages existing in the prior art, and to propose a cell collection device which is convenient to use. The device integrates centrifugation and culture dishes, can adjust the appropriate temperature in an incubator for cell collection, the supernatant and cells after centrifugation can be quickly separated and processed, and the cells and supernatant can be more accurately placed in different small culture dishes as required for cell co-culture.

[0006] To achieve the above purpose, the utility model provides the following technical solution: A cell collection device which is convenient to use, including an incubator and a storage test tube. On both sides of the inner part of the incubator, a plurality of small culture dishes are arranged. On both sides of the inner wall of each small culture dish in the plurality of small culture dishes, near the center, convex blocks are fixedly arranged. Above the two convex blocks of each small culture dish in the plurality of small culture dishes, a second filter plate is arranged. Near the center of the inner side of the inner wall of each small culture dish, a blanking block is fixedly arranged. Above the bottom end of the incubator, a centrifuge is fixedly arranged. Two test tube slots penetrate through the slope at the upper end of the centrifuge. At the center of the rear part of the upper end of the incubator, a top cover is rotatably arranged. An outer glass penetrates through the inside of the top cover. A control panel penetrates through the center of the inside of the top cover.

[0007] The inner wall of the storage test tube is provided with an ultrafiltration membrane, the upper end of the storage test tube is provided with a test tube plug, a filter plate No. 1 is fixedly provided inside the storage test tube near the outer side of the ultrafiltration membrane, telescopic rods are provided on both sides of the lower end of the plate below the No. 1 filter plate inside the storage test tube, springs are provided inside the two telescopic rods, a sliding block is fixedly provided between the lower ends of the slidable parts of the two telescopic rods, and an opening is provided at the lower end of the storage test tube directly below the sliding block;

[0008] Through the above technical solution, centrifugation is carried out in a constant temperature box and storage can be performed immediately after centrifugation. The centrifugation process can ensure the required temperature of the cells to avoid cell death during centrifugation. The storage tube stores the liquid containing the cells just extracted. When centrifuging, the supernatant will be filtered out from the ultrafiltration membrane and the first filter plate under the action of centrifugation. At this time, the ultrafiltration membrane contains the filtered cells, thereby achieving separation of the cells and the supernatant. After separation, it is only necessary to align the opening of the storage tube with the raised part of the feed block, which will support the sliding block. At this time, the sliding block will compress the two telescopic rods to move upward, and the supernatant inside will flow into the small culture dish.

[0009] Furthermore, the outer glass is heat-insulating glass, and is provided with a rubber ring around it, the size of which is consistent with the size of the groove at the upper end of the thermostat;

[0010] Through the above technical solution, the condition of internal cells can be preliminarily observed and processed in time.

[0011] Furthermore, the first filter plate and the second filter plate can filter the cell supernatant but cannot filter the cells;

[0012] Through the above technical solution, the supernatant can be better separated from the cells, and the No. 1 filter plate provides certain support for the ultrafiltration membrane, and cooperates to complete the filtration of the supernatant affected by the centrifugal effect.

[0013] Furthermore, after the ultrafiltration membrane is placed on the inner wall of the storage test tube, its upper end is pressed tightly by the test tube stopper and the storage test tube;

[0014] Through the above technical solution, the ultrafiltration membrane can be arranged and taken out more conveniently, saving time.

[0015] Furthermore, the main material of the ultrafiltration membrane is polyethersulfone, which can filter out the supernatant but not the cells;

[0016] Through the above technical solution, the ultrafiltration membrane made of polyethersulfone material has a stable pore structure and good chemical resistance.

[0017] Furthermore, the temperature inside the thermostat can be monitored and adjusted in real time through a control panel;

[0018] The above technical solution provides a good environment for cell culture and centrifugation, avoiding cell death due to insufficient temperature requirements during the separation process.

[0019] Furthermore, a protrusion is provided at the upper end of the blanking block, and the size of the protrusion is consistent with the size of the opening;

[0020] Through the above technical solution, the supernatant can be quickly taken out without a pipette, thereby improving efficiency.

[0021] The utility model has the following beneficial effects:

[0022] 1. The utility model proposes a cell collection device that is easy to use. The device integrates the cell collection device and the culture dish, is easy to use, and the internal centrifugation process is also carried out in an environment suitable for cell culture. The transfer can be performed immediately after centrifugation. The supernatant of one cell can be quickly introduced into the small culture dish through the feed block, and another cell can be quickly taken out through the ultrafiltration membrane and placed in a small culture dish containing supernatants of different cells. There is no need to use a pipette to suck out, which simplifies the process and improves efficiency, making it easier to complete the co-culture of cells in the cell culture dish.

[0023] 2. The utility model proposes an easy-to-use cell collection device, which adopts a storage tube, cooperates with an ultrafiltration membrane and a No. 1 filter plate under the action of centrifugation to complete the separation of supernatant and cells inside the storage tube, thereby improving the separation efficiency, avoiding incomplete absorption of supernatant when using a pipette, reducing the supernatant residue in the same type of cells, and improving the separation accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a front axonometric schematic diagram of the utility model;

[0025] Figure 2 It is a rear axonometric schematic diagram of the utility model;

[0026] Figure 3 It is a front cross-sectional schematic diagram of a storage test tube of the utility model;

[0027] Figure 4 It is a partial front cross-sectional schematic diagram of the utility model near the test tube slot area.

[0028] Legend:

[0029] 1. Constant temperature box; 2. Centrifuge; 3. Feed block; 4. Small culture dish; 5. Test tube slot; 6. Control panel; 7. Outer glass; 8. Top cover; 9. Test tube plug; 10. Ultrafiltration membrane; 11. Storage test tube; 12. Filter plate No. 1; 13. Telescopic rod; 14. Opening; 15. Sliding block; 16. Spring; 17. Filter plate No. 2; 18. Bump. DETAILED DESCRIPTION

[0030] Next, in combination with the drawings in the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] Referring to Figures 1-4 , an embodiment provided by the present invention: a cell collection device convenient to use, including a constant temperature box 1 and a storage test tube 11. A plurality of small culture dishes 4 are arranged on both sides inside the constant temperature box 1. The plurality of small culture dishes 4 are classified and placed. The supernatant of one kind of cell and another kind of cell are co-cultured in the same small culture dish 4. On both sides of the inner wall of each small culture dish 4 in the plurality of small culture dishes 4, near the center, there are fixed convex blocks 18. Above the two convex blocks 18 of each small culture dish 4 inside the plurality of small culture dishes 4, there is a second filter plate 17 arranged. When the ultrafiltration membrane 10 has undergone centrifugal separation, there are only cells in the ultrafiltration membrane 10. At this time, the ultrafiltration membrane 10 is placed on the second filter plate 17. When placing, the edge of the ultrafiltration membrane 10 forms a concave shape due to the size of the small culture dish 4, and the internal cells will not leak out;

[0032] At the center near the inner side of the inner wall of each of the plurality of small culture dishes 4, there is a fixed material discharging block 3. Aligning the opening 14 of the storage test tube 11 with the protruding part of the material discharging block 3 can push up the sliding block 15, and at this time, the supernatant will immediately flow into the small culture dish 4;

[0033] At the upper end of the bottom of the constant temperature box 1, there is a fixed centrifuge 2. Two test tube slots 5 are arranged through the slope at the upper end of the centrifuge 2. At the center of the rear part of the upper end of the constant temperature box 1, there is a rotatable top cover 8. An outer glass 7 is arranged through the inside of the top cover 8. At the center of the inside of the top cover 8, there is a control panel 6 arranged through. The control panel 6 can control the rotation speed and rotation time of the centrifuge 2, and the internal temperature is controllable;

[0034] An ultrafiltration membrane 10 is arranged on the inner wall of the storage test tube 11. The ultrafiltration membrane 10 is made of polyethersulfone material, which has a stable pore structure and good chemical resistance. After centrifugation, it can filter out the supernatant and retain the cells. A test tube plug 9 is arranged at the upper end of the storage test tube 11. The ultrafiltration membrane 10 is fixed by the closure of the test tube plug 9 and the storage test tube 11. At the outside of the ultrafiltration membrane 10 inside the storage test tube 11, there is a first filter plate 12 fixed;

[0035] On both sides near the lower end of the plate inside the storage test tube 11, below the first filter plate 12, telescopic rods 13 are provided. Springs 16 are arranged inside both telescopic rods 13. A sliding block 15 is fixedly arranged between the lower ends of the slidable parts of the two telescopic rods 13. An opening 14 is provided at the lower end of the storage test tube 11 directly below the sliding block 15.

[0036] Centrifugation is carried out in the incubator 1. After centrifugation, it can be stored immediately. The centrifugation process can ensure the temperature required by the cells and avoid cell death during centrifugation. The storage test tube 11 stores the freshly extracted liquid containing cells. During centrifugation, the supernatant will filter out from the ultrafiltration membrane 10 and the first filter plate 12 under the action of centrifugation. At this time, the inside of the ultrafiltration membrane 10 is the filtered cells, realizing the separation of cells and supernatant. After separation, just align the opening 14 of the storage test tube 11 with the protruding part of the blanking block 3. This protrusion will push against the sliding block 15. At this time, the sliding block 15 will compress the two telescopic rods 13 and move upward. At this time, the internal supernatant will flow into the small culture dish 4.

[0037] The outer glass 7 is heat-insulating glass, and a rubber ring is arranged around it. Its size is the same as the size of the upper groove of the incubator 1, enabling the initial observation of the internal cell condition and timely treatment. The first filter plate 12 and the second filter plate 17 can filter the cell supernatant but cannot filter the cells, enabling the supernatant to be better separated from the cells. The first filter plate 12 provides certain support for the ultrafiltration membrane 10 and cooperates to complete the filtration of the supernatant under the influence of centrifugation. After the ultrafiltration membrane 10 is placed on the inner wall of the storage test tube 11, its upper end is pressed tightly by the test tube stopper 9 and the storage test tube 11, making it more convenient to arrange and remove the ultrafiltration membrane 10 and saving time.

[0038] The main material of the ultrafiltration membrane 10 is polyethersulfone, which can filter out the supernatant but not the cells. The polyethersulfone ultrafiltration membrane 10 has a stable pore structure and good chemical resistance. The internal temperature of the incubator 1 can be monitored and adjusted in real time through the control panel 6, providing a good environment for cell culture and centrifugation and avoiding cell death due to insufficient temperature requirements during separation. The upper end of the blanking block 3 is provided with a protrusion, and the size of the protrusion is the same as the size of the opening 14, enabling the supernatant to be quickly taken out without a pipette and improving efficiency.

[0039] Working principle: When in use, first place the ultrafiltration membrane 10 inside the storage test tube 11. A part of the ultrafiltration membrane 10 extends beyond the storage test tube 11. Put the liquid to be separated and extracted into the storage test tube 11, then plug the test tube stopper 9 into the mouth of the storage test tube 11, and at the same time fix the ultrafiltration membrane 10. Then place the storage test tube 11 in the test tube slot 5, and then close the top cover 8 and adjust a series of parameters such as the rotation speed for centrifugation. After centrifugation, the supernatant will be filtered out outside the ultrafiltration membrane 10, and only cells are contained inside the ultrafiltration membrane 10. Open the top cover 8, take out the storage test tube 11 and remove the test tube stopper 9. Place the ultrafiltration membrane 10 above the second filter plate 17 and ensure that the ultrafiltration membrane 10 is in a concave shape as a whole to prevent cell leakage. Then press the opening 14 of the storage test tube 11 against the protruding part of the blanking block 3. At this time, the sliding block 15 moves upward, and the supernatant flows into the small culture dish 4 through the opening 14. Each small culture dish 4 contains the supernatant of one kind of cell and another kind of cell for co-culture.

[0040] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell collection device that is easy to use, comprising a constant temperature box (1) and a storage test tube (11), characterized in that: A plurality of small culture dishes (4) are arranged on both sides of the interior of the thermostatic box (1); a protrusion (18) is fixedly arranged at the center of both sides of the inner wall of each of the plurality of small culture dishes (4); a No. 2 filter plate (17) is arranged at the upper end of two protrusions (18) located in each of the plurality of small culture dishes (4); a material discharge block (3) is fixedly arranged at the inner center of the inner wall of each of the plurality of small culture dishes (4); a centrifuge (2) is fixedly arranged at the upper end of the bottom of the thermostatic box (1); two test tube slots (5) are penetrated through the slope at the upper end of the centrifuge (2); a top cover (8) is rotatably arranged at the rear center of the upper end of the thermostatic box (1); an outer glass (7) is penetrated through the interior of the top cover (8); and a control panel (6) is penetrated through the interior of the top cover (8); An ultrafiltration membrane (10) is arranged on the inner wall of the storage test tube (11), a test tube plug (9) is arranged on the upper end of the storage test tube (11), a filter plate (12) is fixedly arranged inside the storage test tube (11) near the outer side of the ultrafiltration membrane (10), telescopic rods (13) are arranged on both sides of the lower end of the plate below the first filter plate (12) inside the storage test tube (11), springs (16) are arranged inside the two telescopic rods (13), a sliding block (15) is fixedly arranged between the lower ends of the slidable parts of the two telescopic rods (13), and an opening (14) is opened at the lower end of the storage test tube (11) directly below the sliding block (15).

2. A cell collection device that is easy to use according to claim 1, characterized in that: The outer glass (7) is heat-insulating glass and is provided with a rubber ring around its periphery, the size of which is consistent with the size of the groove at the upper end of the thermostatic box (1).

3. A cell collection device that is easy to use according to claim 1, characterized in that: The first filter plate (12) and the second filter plate (17) can filter the cell supernatant but cannot filter the cells.

4. A cell collection device that is easy to use according to claim 1, characterized in that: After the ultrafiltration membrane (10) is placed on the inner wall of the storage test tube (11), the upper end of the ultrafiltration membrane (10) is pressed tightly by the test tube plug (9) and the storage test tube (11).

5. A cell collection device that is easy to use according to claim 1, characterized in that: The main material of the ultrafiltration membrane (10) is polyethersulfone, which can filter out the supernatant but cannot filter out the cells.

6. A convenient cell collection device according to claim 1, characterized in that: The internal temperature of the thermostatic box (1) can be monitored and adjusted in real time via a control panel (6).

7. A convenient cell collection device according to claim 1, characterized in that: The upper end of the blanking block (3) is provided with a protrusion, and the size of the protrusion is consistent with the size of the opening (14).

Images