Polymerase chain reaction kit

Abstract

The utility model relates to the field of reaction kits, and discloses a polymerase chain reaction kit which comprises a kit body, a kit cover is arranged at the top of the kit body, a plurality of lock catches are installed between the kit body and the kit cover, a lifting assembly is arranged on the lower portion of the kit body, a pressing rod is arranged in the middle of the lifting assembly, and the pressing rod is connected with the lifting assembly. A plurality of placing assemblies are arranged on the upper portion of the lifting assembly, the lifting assembly comprises a top plate, the pressing rod is fixedly connected with the top plate, sliding blocks are fixedly connected to the left side and the right side of the top plate, and first springs are jointly and fixedly connected between the four corners of the bottom face of the top plate and the lower portion of the inner wall of the box body. According to the test tube lifting device, the lifting assembly and the box body are matched with each other, so that the lifting assembly can be adjusted only by pressing the pressing rod, the test tubes are driven to ascend and descend, the test tubes are more convenient to place and take, and the test tube lifting device is more practical.

Description

Technical Field

[0001] The utility model relates to the field of reaction kits, in particular to a polymerase chain reaction kit. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to replicate DNA fragments in vitro and amplify them into a large amount of DNA for further research and analysis. The process of PCR consists of DNA polymerase (usually a heat-stable DNA polymerase such as Taq polymerase), DNA template, two primers (used to initiate the extension of the newly synthesized DNA strand), dNTPs (deoxynucleotide triphosphates, i.e., the four base monomers required for DNA synthesis), buffer, and magnesium ions.

[0003] After retrieval, Chinese Patent Publication No.: CN219313374U discloses a PCR kit, including a box body. A group of test tube slots are opened inside the box body. A group of limiting plates are fixedly connected to the bottom of each test tube slot. A clamping slot is opened on the left side of each limiting plate, and a rising slot is opened on the right side of each limiting plate. A limiting slot is opened at the bottom of each test tube slot. A limiting block is slidably connected inside each limiting slot. A pressing block is fixedly connected to the outer surface of each limiting block. In this PCR kit, through the cooperation among the sliding block, the limiting plate, the clamping slot, and the rising slot, when the sliding block moves upward under the thrust of the spring, the limiting plate restricts its movement route, causing it to rotate while moving upward. After rotation, the top of the sliding block is restricted by the clamping slot, thereby keeping the test tube in the test tube slot and avoiding the situation that the test tube is broken due to collision during transportation.

[0004] Although the above device can protect the test tube and facilitate the taking and placing of the test tube by setting the cooperation among the sliding block, the limiting plate, the clamping slot, and the rising slot, when placing or taking out the test tube, it is necessary to press each test tube one by one to retract the test tube into the test tube slot or to make the spring eject the test tube, which is time-consuming. Therefore, a polymerase chain reaction kit is proposed to solve the above problems. Summary of the Utility Model

[0005] In order to make up for the above deficiencies, the utility model provides a polymerase chain reaction kit, aiming to improve the problem that in the prior art, when placing or taking out the test tube, it is necessary to press each test tube one by one to retract the test tube into the test tube slot or to make the spring eject the test tube, which is time-consuming.

[0006] To achieve the above object, the present utility model adopts the following technical solutions: A polymerase chain reaction kit, comprising a box body, a box cover is arranged on the top of the box body, a plurality of lock catches are commonly installed between the box body and the box cover, a lifting component is arranged at the lower part of the box body, a pressing rod is arranged in the middle of the lifting component, a plurality of placing components are arranged at the upper part of the lifting component, the lifting component includes a top plate, and the pressing rod is fixedly connected to the top plate.

[0007] As a further description of the above technical solution:

[0008] Sliders are fixedly connected to both the left and right sides of the top plate, first springs are commonly fixedly connected between the four corners of the bottom surface of the top plate and the lower part of the inner wall of the box body, and a lifting member is installed in the middle of the bottom surface of the top plate.

[0009] As a further description of the above technical solution:

[0010] The lifting member includes a connecting block, control grooves are formed on both the left and right sides of the connecting block, a plurality of second springs are commonly fixedly connected between the connecting block and the bottom of the inner wall of the box body, and connecting members are arranged on both the left and right sides of the connecting block.

[0011] As a further description of the above technical solution:

[0012] The connecting member includes a support rod, connecting rods are fixedly connected to one side ends of the support rod close to the control groove, the upper connecting rod is slidably connected to the control groove, the outer wall of the lower connecting rod penetrates and is rotatably connected to a fixing block, and the fixing block is fixedly connected to the bottom of the inner wall of the box body.

[0013] As a further description of the above technical solution:

[0014] Chute grooves are formed on both the left and right sides of the inner wall of the box body, and the sliders are slidably connected thereto.

[0015] As a further description of the above technical solution:

[0016] The placing component includes a placing tube, the placing tube is fixedly connected to the top plate, and a plurality of fixing members are arranged inside the placing tube.

[0017] As a further description of the above technical solution:

[0018] The fixing member includes an empty rod, the empty rod is fixedly connected to the inner wall of the placing tube, a sliding rod is slidably connected to one side of the empty rod away from the placing tube, and a plurality of third springs are commonly fixedly connected between the sliding rod and the empty rod.

[0019] As a further description of the above technical solution:

[0020] One side of the sliding rod away from the hollow rod is fixedly connected with a limiting strip.

[0021] The utility model has the following beneficial effects:

[0022] 1. In the utility model, through the mutual cooperation between the lifting assembly and the box body, the device can adjust the lifting assembly only by pressing the pressing rod, thereby driving the lifting of the test tube, which is more convenient for placing and taking the test tube, and is more practical.

[0023] 2. In the utility model, by setting the placing assembly, through the mutual cooperation between the placing tube and the multiple fixing pieces, the device can fix test tubes of different sizes, making the fixing of the test tube more convenient and effectively improving the practicability of the device. Description of the Drawings

[0024] Figure 1 It is an overall three-dimensional structure schematic diagram of a polymerase chain reaction kit proposed by the utility model;

[0025] Figure 2 It is an internal three-dimensional structure schematic diagram of a polymerase chain reaction kit proposed by the utility model;

[0026] Figure 3 It is a split three-dimensional structure schematic diagram of the fixing piece of a polymerase chain reaction kit proposed by the utility model;

[0027] Figure 4 It is a three-dimensional structure schematic diagram of the lifting assembly of a polymerase chain reaction kit proposed by the utility model;

[0028] Figure 5 It is a split three-dimensional structure schematic diagram of the lifting piece of a polymerase chain reaction kit proposed by the utility model.

[0029] Legend Explanation:

[0030] 1. Box body; 2. Box cover; 3. Lock; 4. Placing assembly; 5. Pressing rod; 6. Lifting assembly; 61. Top plate; 62. Slide block; 63. First spring; 64. Lifting piece; 641. Connecting block; 642. Control groove; 643. Second spring; 644. Support rod; 645. Connecting rod; 646. Fixed block; 11. Chute; 41. Placing tube; 42. Fixing piece; 421. Hollow rod; 422. Sliding rod; 423. Limiting strip; 424. Third spring. Detailed Embodiment

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0032] Referring to Figure 1 - Figure 2 , an embodiment provided by the present invention: a polymerase chain reaction kit, including a box body 1 for connecting various components. Sliding grooves 11 are opened on the left and right sides of the inner wall of the box body 1 for cooperating with the connection of the lifting component 6, and a slider 62 is slidably connected thereto. A box cover 2 is provided on the top of the box body 1 for cooperating with the box body 1. A plurality of lock catches 3 are commonly installed between the box body 1 and the box cover 2 for cooperating with the opening and closing between the box body 1 and the box cover 2. A lifting component 6 is provided at the lower part of the box body 1 for cooperating with the pressing and taking of reagent tubes. A pressing rod 5 is provided in the middle of the lifting component 6 for cooperating with adjusting the lifting member 64. A plurality of placing components 4 are provided at the upper part of the lifting component 6 for placing reagent tubes.

[0033] As Figure 4 - Figure 5 shown, the lifting component 6 includes a top plate 61 for connecting various components. The pressing rod 5 is fixedly connected to the top plate 61. Sliders 62 are fixedly connected to the left and right sides of the top plate 61 for cooperating with the connection between the top plate 61 and the box body 1. First springs 63 are commonly fixedly connected between the four corners of the bottom surface of the top plate 61 and the lower part of the inner wall of the box body 1 for cooperating with the lifting of the top plate 61. A lifting member 64 is installed in the middle of the bottom surface of the top plate 61 for adjusting the lifting of the top plate 61.

[0034] Further, the lifting member 64 includes a connecting block 641 for connecting various components. Control grooves 642 are formed on both the left and right sides of the connecting block 641. The shape of the control grooves 642 is similar to a heart shape. It is not a symmetric figure and has certain undulating changes. This is to facilitate the sliding trajectory of the control connecting rod 645 within the control grooves 642, enabling the lifting assembly 6 to drive the placement assembly 4 to lift. Depressions for limiting the connecting rod 645 are provided at both the upper and lower parts of the control grooves 642, facilitating the fixing of the position of the connecting block 641 by the connecting member. A plurality of second springs 643 are fixedly connected between the connecting block 641 and the bottom inner wall of the box body 1 to cooperate with the movement of the connecting block 641. Connecting members are provided on both the left and right sides of the connecting block 641 to cooperate with the movement and limitation of the control of the connecting block 641. The connecting member includes a support rod 644 for cooperating with the control of the connecting block 641. Connecting rods 645 are fixedly connected to the side ends of the support rod 644 close to the control grooves 642. The connecting rod 645 located at the upper part is slidably connected to the control groove 642 for connecting the support rod 644 and the control groove 642. The outer wall of the connecting rod 645 located at the lower part penetrates and is rotatably connected to a fixing block 646 for cooperating with the connection and limitation of the support rod 644. The fixing block 646 is fixedly connected to the bottom inner wall of the box body 1.

[0035] As Figure 2 - Figure 3 As shown, the placement assembly 4 includes a placement tube 41, the height of which is slightly shorter than that of a test tube for cooperating with the placement of a test tube. The placement tube 41 is fixedly connected to the top plate 61. A plurality of fixing members 42 are arranged inside the placement tube 41 for fixing the test tube. The fixing member 42 includes an empty rod 421 for cooperating with a sliding rod 422. The empty rod 421 is fixedly connected to the inner wall of the placement tube 41. A sliding rod 422 is slidably connected to the side of the empty rod 421 away from the placement tube 41 for adapting to the fixing of test tubes of different sizes. A plurality of third springs 424 are fixedly connected between the sliding rod 422 and the empty rod 421 to cooperate with the movement and reset of the sliding rod 422 to assist in fixing the test tube. A limiting strip 423 made of rubber is fixedly connected to the side of the sliding rod 422 away from the empty rod 421 for cooperating with the fixing of the test tube.

[0036] Working principle: When in use, open the lock 3 between the box body 1 and the box cover 2, separate the box cover 2 from the box body 1, and then insert the test tube to be placed into the placement tube 41. When the test tube is inserted into the placement tube 41, it will exert pressure on the fixing member 42. The sliding rod 422 will move towards the empty rod 421 and exert pressure on the third spring 424. At the same time, the third spring 424 will act on the sliding rod 422 in the opposite direction, pushing it outwards so that the limiting strip 423 clamps and fixes the test tube. After the test tube is fixed, cover the box cover 2 and the box body 1 and fasten the lock 3.

[0037] When it is necessary to take out the test tube, open the box cover 2 again, and then press down the pressing rod 5. When the pressing rod 5 is pressed down, it will drive the top plate 61 to move downward accordingly. The slider 62 connected to the top plate 61 will slide along the chute 11, and the connecting rod 645 will move downward along the direction of the control groove 642. When the connecting rod 645 reaches the depression at the lower part of the control groove 642, the upper part of the connecting block 641 will lose the pressure exerted by the connecting piece, and the second spring 643 will push the connecting block 641 upward accordingly to reset it. At the same time, the connecting rod 645 will be stuck in the depression at the lower part of the control groove 642 to fix the position of the connecting block 641. When the connecting block 641 moves upward, with the assistance of the first spring 63 and the second spring 643, it will push the top plate 61 upward. The placement component 4 on the upper part of the top plate 61 will be lifted upward, and the test tube inside it will be pushed upward, making its upper part higher than the top of the box body 1, which is convenient for taking the test tube. When the test tube has been taken, and it is necessary to retract and fix the lifting component 6 downward, press the pressing rod 5 again, and the top plate 61 will drive the connecting block 641 to move downward accordingly. When the connecting block 641 moves downward, the control groove 642 will drive the connecting rod 645 inside it to slide backward and upward. The connecting rod 645 will continue to move along the shape of the control groove 642 until it is stuck in the depression at the upper part of the control groove 642, thereby fixing the position of the connecting block 641. At this time, the connecting block 641 will squeeze the second spring 643, and the top plate 61 will also squeeze the first spring 63. At this time, the lifting component 6 retracts downward, which is convenient for subsequent placement and storage of the test tube.

[0038] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A polymerase chain reaction kit, comprising a box body (1), characterized in that: A box cover (2) is arranged on the top of the box body (1), a plurality of lock buckles (3) are installed between the box body (1) and the box cover (2), a lifting assembly (6) is arranged at the bottom of the box body (1), a push rod (5) is arranged at the middle of the lifting assembly (6), a plurality of placement assemblies (4) are arranged at the top of the lifting assembly (6), the lifting assembly (6) comprises a top plate (61), and the push rod (5) is fixedly connected to the top plate (61).

2. A polymerase chain reaction kit according to claim 1, characterized in that: The left and right sides of the top plate (61) are fixedly connected with sliders (62), the four corners of the bottom surface of the top plate (61) and the lower part of the inner wall of the box body (1) are fixedly connected with a No. 1 spring (63), and the middle part of the bottom surface of the top plate (61) is installed with a lifting member (64).

3. A polymerase chain reaction kit according to claim 2, characterized in that: The lifting member (64) comprises a connecting block (641), control grooves (642) are provided on the left and right sides of the connecting block (641), a plurality of No. 2 springs (643) are fixedly connected between the connecting block (641) and the bottom of the inner wall of the box body (1), and connecting members are provided on the left and right sides of the connecting block (641).

4. A polymerase chain reaction kit according to claim 3, characterized in that: The connecting member comprises a support rod (644), one side end of the support rod (644) close to the control groove (642) is fixedly connected to a connecting rod (645), the connecting rod (645) at the upper part is slidably connected to the control groove (642), and a fixing block (646) is penetrated through the outer wall of the connecting rod (645) at the lower part and is rotatably connected, and the fixing block (646) is fixedly connected to the bottom of the inner wall of the box body (1).

5. A polymerase chain reaction kit according to claim 2, characterized in that: The left and right sides of the inner wall of the box body (1) are both provided with sliding grooves (11), and the sliding block (62) is slidably connected thereto.

6. A polymerase chain reaction kit according to claim 1, characterized in that: The placement assembly (4) comprises a placement tube (41), the placement tube (41) is fixedly connected to the top plate (61), and a plurality of fixing members (42) are provided inside the placement tube (41).

7. A polymerase chain reaction kit according to claim 6, characterized in that: The fixing member (42) comprises a hollow rod (421), wherein the hollow rod (421) is fixedly connected to the inner wall of the placement tube (41), and a sliding rod (422) is slidably connected to the side of the hollow rod (421) away from the placement tube (41), and a number of No. 3 springs (424) are fixedly connected between the sliding rod (422) and the hollow rod (421).

8. A polymerase chain reaction kit according to claim 7, characterized in that: The side of the sliding rod (422) away from the hollow rod (421) is fixedly connected to the limiting strip (423).

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