Pet disease detection device

Abstract

The utility model relates to the technical field of biological detection, and discloses a pet disease detection device which comprises a reagent tube, the top end of the reagent tube is open, a reaction chamber is arranged in the reagent tube, and a first reagent is stored in the reaction chamber; the reagent cover is detachably connected to the top end of the reagent tube, the bottom end of the reagent cover is provided with an opening and covered with a sealing film, the reagent cover and the sealing film are sealed to form a reagent cavity, and a second reagent is stored in the reagent cavity; the puncture needle penetrates through the top end of the reagent cover; the reagent cover in which the second reagent is hermetically stored is hermetically connected with the reagent tube in which the first reagent is stored, an object to be detected firstly reacts with the first reagent, and the sealing film is punctured by the puncture needle, so that the second reagent in the reagent cover drips into the reagent tube to perform a second reaction, and the whole detection process is performed in a closed system; the risk of nucleic acid pollution possibly caused by multiple operations is effectively avoided, so that the accuracy of a detection result is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, and particularly relates to a pet disease detection device. Background Art

[0002] With the continuous improvement of living standards, pets have become an indispensable part of people's lives, and their health conditions have also received close attention from pet owners. However, there are a wide variety of pet diseases. For example, common pet cats and dogs are infected with feline herpesvirus, feline calicivirus, canine distemper virus, canine parainfluenza virus, etc., and the symptoms are not very different, which brings great difficulties to the early detection and diagnosis of pet diseases.

[0003] At present, although there are some test kits on the market that can detect pet diseases, these test kits often require multiple detection tools to be used in combination. Moreover, the detection combining the existing novel isothermal amplification technology and CRISPR needs to be operated step by step in separate tubes, and the opening step is prone to nucleic acid contamination, affecting the detection results.

[0004] Therefore, it is necessary to provide a new type of pet disease detection kit to solve the above problems. Summary of the Utility Model

[0005] The purpose of the utility model is to provide a pet disease detection device to solve the technical problem of the need for step-by-step detection with the lid open in the prior art.

[0006] The utility model adopts the following technical solutions:

[0007] A pet disease detection device includes:

[0008] A reagent tube, the top of the reagent tube is open, and a reaction chamber is arranged in the reagent tube, and a first reagent is stored in the reaction chamber;

[0009] A reagent cap, the reagent cap is detachably connected to the top of the reagent tube, the bottom end of the reagent cap is provided with an opening and covered with a sealing film, and the reagent cap and the sealing film are sealed to form a reagent chamber, and a second reagent is stored in the reagent chamber;

[0010] A puncture needle, penetrating the top of the reagent cap, and a pressing part is arranged at one end of the puncture needle away from the reagent cap.

[0011] Further, the reagent tube is provided with an internal thread, and the reagent cap is provided with an external thread corresponding to the internal thread, so that the reagent cap is detachably connected to the top of the reagent tube.

[0012] Further, a through hole is formed at the top end of the reagent cap, the puncture needle is disposed in the through hole, a puncture portion is provided at the bottom of the puncture needle, and the cross-sectional area of the puncture portion is larger than that of the through hole.

[0013] Further, a safety lever is disposed below the pressing portion, the safety lever is fixedly connected to the puncture needle, and the reagent cap is provided with a movable groove for the safety lever to slide.

[0014] Further, an elastic member is further included, the elastic member is disposed through the puncture needle, and two ends of the elastic member are respectively connected to the bottom end of the safety lever and the bottom wall of the movable groove.

[0015] Further, a mounting bracket for fixing the reagent tube is further included, the mounting bracket is disposed at the bottom end of the reagent tube, a hinge disc is disposed on one side of the mounting bracket, the hinge disc is connected with a rotary telescopic rod, the rotary telescopic rod is provided with a hinge ball connected to the hinge disc, and the rotary telescopic rod is used for reciprocating rotary lifting of the mounting bracket.

[0016] Further, a base is further included, a guide rod is disposed at the upper end of the base, the guide rod is disposed through the mounting bracket, so that the mounting bracket reciprocates rotary lifting on the guide rod.

[0017] Further, a support frame is further included, the support frame is disposed on the base and the support frame is sleeved outside the rotary telescopic rod to support and limit the rotary telescopic rod.

[0018] Further, a driving assembly is further included, the driving assembly includes a pulley and a driving member, the pulley is sleeved outside the rotary telescopic rod, a driving wheel is disposed on the output shaft of the driving member, and the driving wheel is in transmission connection with the pulley through a belt.

[0019] Further, a spectrometer is further included, a detection window is formed on the mounting bracket, the spectrometer is disposed on the base and the detection port of the spectrometer corresponds to the detection window.

[0020] Advantageous effects:

[0021] A pet disease detection device provided by the present utility model seals and connects a reagent cap storing a second reagent with a reagent tube storing a first reagent, first reacts a test substance with the first reagent, punctures a sealing film through a puncture needle, so that the second reagent in the reagent cap drips into the reagent tube for a second reaction, and the whole detection process is carried out in a closed system, without step-by-step and tube-by-tube operation, effectively avoiding the risk of nucleic acid contamination possibly brought by multiple operations, thereby being able to ensure the accuracy of the detection result. Description of the Drawings

[0022] Figure 1 Schematic diagram of the overall structure of an embodiment of a pet disease detection device of the present utility model;

[0023] Figure 2 Schematic cross-sectional structure diagram of a reagent tube of a pet disease detection device of the present utility model;

[0024] Figure 3 Schematic structure diagram of a reagent cap of a pet disease detection device of the present utility model;

[0025] Figure 4 Schematic cross-sectional view of a reagent cap of a pet disease detection device of the present utility model;

[0026] Wherein: 1. Reagent tube; 2. First reagent; 3. Reagent cap; 4. Sealing film; 5. Second reagent; 6. Puncture needle; 61. Pressing part; 62. Puncturing part; 63. Safety rod; 7. Activity groove; 8. Elastic member; 9. Mounting rack; 10. Hinge disc; 11. Rotating telescopic rod; 12. Hinge ball; 13. Base; 14. Guide rod; 15. Support frame; 16. Pulley; 17. Driving member; 18. Driving wheel; 19. Spectrometer.

[0027] The realization, functional features and advantages of the purpose of the present utility model will be further described with reference to the embodiments and the accompanying drawings. Specific embodiments

[0028] It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0029] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of the described features. In the description of the present utility model, the meaning of "a number of" is two or more, unless otherwise specifically defined.

[0030] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, and it may be the communication inside two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0031] In the present utility model, unless otherwise clearly specified and defined, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over", and "on top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "beneath", and "underneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0032] Referring to Figure 2 , the present utility model provides a pet disease detection device, comprising: a reagent tube 1, the top of the reagent tube 1 is open, and a reaction chamber is provided inside the reagent tube 1, and a first reagent 2 is stored in the reaction chamber;

[0033] a reagent cap 3, the reagent cap 3 is detachably connected to the top of the reagent tube 1, an opening is provided at the bottom end of the reagent cap 3 and is covered with a sealing film 4, the reagent cap 3 and the sealing film 4 are sealed to form a reagent chamber, and a second reagent 5 is stored in the reagent chamber;

[0034] a puncture needle 6, penetrating the top of the reagent cap 3, and a pressing portion 61 is provided at one end of the puncture needle 6 away from the reagent cap 3.

[0035] In the above embodiment, the reagent tube 1 is a container with an open top, and a reaction chamber is provided inside, which is specifically configured as a test tube structure, and a first reagent 2 is stored inside. The reagent cap 3 is detachably connected to the top of the reagent tube 1 so that a sealed connection relationship is formed between the reagent tube 1 and the reagent cap 3. An opening is provided at the bottom of the reagent cap 3 and is covered with a sealing film 4. A sealed reagent chamber is jointly formed inside the reagent cap 3 and the sealing film 4 for storing the second reagent 5.

[0036] The puncture needle 6 penetrates through the top of the reagent cap 3 as a whole. A pressing part 61 is provided at its top end. When detection is required, simply press the pressing part 61 to make the puncture needle 6 penetrate through the sealing film 4, so as to release the second reagent 5 in the reagent chamber into the reaction chamber of the reagent tube 1.

[0037] Specifically, the first reagent 2 is a PCR reaction solution, which specifically contains 10 μL of 2X reaction Buffer, 0.8 μL of 8 U / μL Bst2.0 DNA polymerase, 0.4 μL of 16 U / μL RT heat-resistant reverse transcriptase, 2 μL of 10X primer, 2 μL of template DNA, and 4.8 μL of DEPC water, and is used for nucleic acid amplification; the second reagent 5 is a CRISPR cleavage reaction, which specifically contains 0.75 μL of Cas12b with an initial concentration of 10 μM, 1 μL of sgRNA with an initial concentration of 25 μM, 1 μL of reporter probe with an initial concentration of 25 μM, and 2.25 μL of DEPC water.

[0038] The specific operation process is as follows: Add the pet sample to be tested into the reaction chamber in the reagent tube 1, seal and connect the reagent tube 1 and the reagent cap 3, and keep the mixture of the pet sample to be tested and the first reagent 2 at a constant temperature of 65 °C for 30 minutes for mixing reaction. After the timing is completed, press the pressing part 61 of the puncture needle 6 to make the tip of the puncture needle 6 penetrate through the sealing film 4 of the reagent cap 3, so as to drip the second reagent 5 in the reagent cap 3 into the reagent tube 1, and keep the mixture of the pet sample to be tested, the first reagent 2 and the second reagent 5 at a temperature of 65 °C for reaction for 10 minutes. During this period, fluorescence data of the reagent tube 1 is collected once per minute.

[0039] Through the above structure, the nucleic acid release and PCR amplification reaction of the sample are realized in a closed system, without the need for step-by-step and separate tube operations, effectively avoiding the risk of nucleic acid contamination that may be brought by multiple operations, thereby ensuring the accuracy of the detection results. The detachable design of the reagent tube 1 and the reagent cap 3 makes the repeated use of the device simple and convenient. The reagent cap 3 can be used once. After being punctured and used, it can be replaced by removing the reagent cap 3 from the reagent tube 1. At the same time, the reagent tube 1 can be thoroughly cleaned and disinfected for the next use.

[0040] In addition, the types and combinations of reagents can be changed to meet the detection requirements of different types of pet diseases. For example, according to the characteristics of different viruses or bacteria, different reagents can be selected for combination to achieve accurate detection of specific diseases.

[0041] In one embodiment, the reagent tube 1 is provided with an internal thread, and the reagent cap 3 is provided with an external thread corresponding to the internal thread, so that the reagent cap 3 is detachably connected to the top end of the reagent tube 1.

[0042] In the above embodiment, an internal thread is provided at the mouth of the reagent tube 1, and the reagent cap 3 is equipped with a corresponding external thread. Through the screwing of the internal and external threads, the reagent cap 3 can be firmly connected to the top of the reagent tube 1. This connection method is both simple and reliable, ensuring a tight seal between the reagent tube 1 and the reagent cap 3, preventing the leakage of reagents and the entry of external contaminants. At the same time, the threaded connection also makes it convenient to disassemble the reagent cap 3. When it is necessary to replace the reagent cap 3 or clean the reagent tube 1, simply rotate the reagent cap 3 gently to remove it, and the operation is simple and fast.

[0043] In addition, the use of the threaded connection method also helps to improve the stability and durability of the device. Due to the tightness of the threaded connection, the reagent tube 1 and the reagent cap 3 can form a stable whole after connection and are not easily loosened due to external vibration or impact. This is crucial for ensuring the stability and accuracy of the detection process. At the same time, the threaded connection structure also has strong durability and can withstand multiple disassembly and repeated use, extending the service life of the device.

[0044] In one embodiment, referring to Figure 4 , a through hole is provided at the top end of the reagent cap 3, the puncture needle 6 is arranged in the through hole, a puncture part 62 is provided at the bottom of the puncture needle 6, and the cross-sectional area of the puncture part 62 is larger than that of the puncture hole.

[0045] In the above embodiment, the top end of the reagent cap 3 is designed with a through hole, providing a fixed and precise insertion point for the puncture needle 6. The puncture needle 6 is arranged in the through hole to ensure its stable position and not easy to shake. And a puncture part 62 is provided at the bottom of the puncture needle 6. The cross-sectional area of the puncture part 62 is larger than that of the puncture hole, preventing the incorrect placement of the reagent cap 3 from causing the puncture needle 6 to fall off. At the same time, the larger cross-sectional area of the puncture part 62 increases the contact area with the sealing film 4, enabling more uniform and stable pressure application during the puncture process, avoiding uneven damage to the sealing film 4 or puncture failure caused by uneven force. This greatly improves the accuracy and success rate of the puncture, ensuring that the second reagent 5 in the reagent chamber can flow smoothly and completely into the reaction chamber of the reagent tube 1.

[0046] In one embodiment, referring to Figure 3 and Figure 4 , a safety lever 63 is provided below the pressing part 61. The safety lever 63 is fixedly connected to the puncture needle 6, and the reagent cap 3 is provided with a movable groove 7 for the safety lever 63 to slide.

[0047] In the above embodiment, a safety lever 63 is provided below the pressing part 61. The safety lever 63 is fixedly connected to the puncture needle 6, and the safety lever 63 is mounted on the upper end surface of the reagent cap 3. The movable groove 7 opened on the reagent cap 3 is arranged staggered with the safety lever 63, so that the safety lever 63 cannot slide in the initial stage, restricting the puncture operation. By rotating the puncture needle 6, the safety lever 63 falls into the movable groove 7, providing a sliding space for the safety lever 63, so that during the pressing process, the safety lever 63 can move smoothly along the movable groove 7, and then drive the puncture needle 6 to perform a puncture action. The setting of the safety lever 63 avoids accidental puncture and prevents the second reagent 5 from leaking when not being detected.

[0048] In one embodiment, referring to Figure 4 , an elastic member 8 is further included. The elastic member 8 is inserted through the puncture needle 6, and both ends of the elastic member 8 are respectively connected to the bottom end of the safety lever 63 and the bottom wall of the movable groove 7.

[0049] In the above embodiment, by sleeving an elastic member 8 on the puncture needle 6 and respectively connecting both ends of the elastic member 8 to the bottom end of the safety lever 63 and the bottom wall of the movable groove 7, the automatic reset function of the puncture needle 6 is realized, and further prevents the incorrect placement of the reagent cap 3 from causing the puncture needle 6 to fall off. The elastic member 8 is preferably set as a compression spring. When the puncture needle 6 is rotated so that the safety lever 63 can fall into the movable groove 7, and the pressing part 61 is pressed for puncture, the elastic member 8 is compressed and stores potential energy; when the pressing part 61 is released, the elastic member 8 releases the potential energy, pushing the safety lever 63 and the puncture needle 6 to automatically reset, so that the puncture needle 6 is withdrawn from the sealing film 4 and restored to the initial state, which not only simplifies the operation process, but also improves the detection efficiency and accuracy, enabling the second reagent 5 to quickly drip into the reagent tube 1.

[0050] In one embodiment, referring to Figure 1 , an installation frame 9 for fixing the reagent tube 1 is further included. The installation frame 9 is arranged at the bottom end of the reagent tube 1. A hinge disc 10 is arranged on one side of the installation frame 9. The hinge disc 10 is connected with a rotary telescopic rod 11. The rotary telescopic rod 11 is provided with a hinge ball 12 connected to the hinge disc 10. The rotary telescopic rod 11 is used to make the installation frame 9 reciprocally rotate and lift.

[0051] In the above embodiment, the installation of the mounting frame 9 provides a stable and easy-to-test support platform for the reagent tube 1. A hinge plate 10 is provided on one side of the mounting frame 9, and a hinge ball 12 is extended from the rotating telescopic rod 11 in a direction parallel to the axial direction but not coaxially. The hinge plate 10 is hingedly connected with the hinge ball 12, and the mounting frame 9 is driven to reciprocate and rise and fall through the rapid rotation and passive extension of the rotating telescopic rod 11, thereby realizing the function of efficient rotation and vibration of the mounting frame 9, so that each reagent can fully react with the pet sample to be tested.

[0052] In one embodiment, a base 13 is further included. A guide rod 14 is disposed at the upper end of the base 13 . The guide rod 14 is passed through the mounting frame 9 so that the mounting frame 9 can be reciprocated and rotated and lifted on the guide rod 14 .

[0053] In the above embodiment, the base 13 provides a stable support for the entire device, and the mounting frame 9 has a hollow structure. The guide rod 14 at the upper end of the base 13 cooperates with the mounting frame 9, and the guide rod 14 plays a role in guiding and limiting the degree of freedom, so that the mounting frame 9 can be reciprocated and rotated on the guide rod 14. By speeding up the frequency of this reciprocating rotation and lifting, it becomes a rotational vibration, so that the reagent tube 1 can be efficiently rotated and vibrated during the detection process, thereby accelerating the mixing and reaction of the reagent and the pet sample to be tested. The existence of the guide rod 14 also ensures the stability of the mounting frame 9 during the lifting process, avoiding detection errors caused by shaking or offset.

[0054] In one embodiment, a support frame 15 is further included. The support frame 15 is disposed on the base 13 and sleeved on the outer side of the rotating telescopic rod 11 to support and limit the rotating telescopic rod 11 .

[0055] In the above embodiment, the support frame 15 is installed on the base 13 and is sleeved on the outside of the rotating telescopic rod 11. It is specifically configured as two parts, which are configured in front and behind the rotating telescopic rod 11. This can not only synchronously support the rotating telescopic rod 11 while keeping the rotating telescopic rod 11 able to rotate, but also effectively restrict it to prevent it from shaking or deflecting during work, thereby ensuring the stability of the rotating telescopic rod 11 during high-speed rotation and extension.

[0056] In one embodiment, a driving assembly is further included, and the driving assembly includes a pulley 16 and a driving member 17. The pulley 16 is sleeved on the outer side of the rotating telescopic rod 11, and the output shaft of the driving member 17 is provided with a driving wheel 18, and the driving wheel 18 is connected to the pulley 16 through a belt.

[0057] In the above embodiments, the setting of the driving component provides a power source for the rotation and telescopic functions of the entire device, that is, it provides power for the rotation and vibration of the mounting bracket 9. The pulley 16 is sleeved outside the rotary telescopic rod 11. The specific position of the pulley 16 is between two support frames 15 arranged one in front and the other behind. The driving wheel 18 on the output shaft of the driving member 17 is connected to the pulley 16 through belt drive to achieve power transmission. At the same time, the support frame 15 can also limit the position of the pulley 16. The driving member 17 can specifically adopt a synchronous motor. When the driving member 17 is started, the driving wheel 18 drives the pulley 16 to rotate through the belt, and then drives the rotary telescopic rod 11 to perform a rotational motion. At the same time, since the rotary telescopic rod 11 has a telescopic function, the distance between the rotary telescopic rod 11 and the hinge ball 12 can be adjusted as needed to achieve different vibration and rotation strokes.

[0058] At the same time, by adjusting the rotation speed of the driving member 17 and the telescopic speed of the rotary telescopic rod 11, the rotation and vibration frequency and amplitude of the reagent tube 1 can be controlled to adapt to different types of pet samples and detection requirements, so that the device can be widely used in various pet medical detection scenarios.

[0059] In one embodiment, a spectrometer 19 is further included. The mounting bracket 9 is provided with a detection window. The spectrometer 19 is arranged on the base 13 and the detection port of the spectrometer 19 corresponds to the detection window.

[0060] In the above embodiments, the second reagent 5 contains a fluorescent agent. The spectrometer 19 obtains information such as the properties and components of a substance by detecting the interaction between light and the substance. In this embodiment, the spectrometer 19 preferably adopts an X-ray fluorescence spectrometer. The spectrometer 19 is arranged on the base 13 and corresponds to the sample to be measured through the detection window opened on the mounting bracket 9. The setting of the detection window not only facilitates the detection light of the spectrometer 19 to pass through, but also prevents interference from the external environment and improves the accuracy of detection.

[0061] After the reagent in the reagent tube 1 is fully mixed with the pet sample to be measured and reacts, the detection port of the spectrometer 19 detects the sample through the detection window. The spectrometer 19 can quickly obtain the spectral information of the sample and convert it into analyzable data, so as to realize the qualitative or quantitative analysis of the pet sample.

[0062] Furthermore, a thermostat can be provided outside the reagent tube 1 or at the mounting bracket 9. One detection method is to react the mixture of the pet sample to be tested and the first reagent 2 in the reagent tube 1 at a temperature of 65°C for 30 minutes, and then add the second reagent 5 and continue to react at a constant temperature of 65°C for 10 minutes. During this period, fluorescence data of the reagent tube 1 is collected once per minute. Then, the results are interpreted. The negative control shows no fluorescence signal curve and no Ct value; the positive control shows a fluorescence signal and Ct value ≤ 20 min. The above two judgments need to be satisfied simultaneously in the same experiment. Otherwise, the experiment of that time is invalid and needs to be repeated. When there is a fluorescence signal curve and Ct value ≤ 38 and the quality control is normal, it can be determined as positive for the disease. When there is no fluorescence signal curve, no Ct value or Ct value > 38 and the quality control is normal, it can be determined as negative for the disease. Among them, the Ct value is the time required for the fluorescence signal to reach the set threshold. The setting of the constant temperature is to enable the reagent and the sample to react in a suitable environment to improve the sensitivity and accuracy of the detection. At the same time, by maintaining the constant temperature of the reagent tube 1 through the thermostat, the influence of environmental temperature fluctuations on the experimental results can be reduced, and the reliability of the detection results can be improved.

[0063] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied to other related technical fields, shall be similarly included in the patent protection scope of the present invention.

Claims

1. A pet disease detection device, characterized in that: include: A reagent tube, wherein the top of the reagent tube is open and a reaction chamber is arranged inside the reagent tube, and the reaction chamber stores a first reagent; A reagent cover, wherein the reagent cover is detachably connected to the top of the reagent tube, the bottom of the reagent cover is provided with an opening and covered with a sealing film, the reagent cover and the sealing film are sealed to form a reagent chamber, and a second reagent is stored in the reagent chamber; The puncture needle penetrates the top end of the reagent cover, and a pressing portion is arranged at one end of the puncture needle away from the reagent cover.

2. A pet disease detection device according to claim 1, characterized in that: The reagent tube is provided with an internal thread, and the reagent cover is provided with an external thread corresponding to the internal thread, so that the reagent cover can be detachably connected to the top end of the reagent tube.

3. A pet disease detection device according to claim 1, characterized in that: A penetration hole is formed at the top of the reagent cover, the puncture needle is arranged in the penetration hole, and a puncture portion is formed at the bottom of the puncture needle, and the cross-sectional area of ​​the puncture portion is larger than that of the penetration hole.

4. A pet disease detection device according to claim 1, characterized in that: A safety rod is arranged below the pressing part, the safety rod is fixedly connected to the puncture needle, and the reagent cover is provided with a movable groove for the safety rod to slide.

5. A pet disease detection device according to claim 4, characterized in that: It also includes an elastic member, which is penetrated by the puncture needle and has two ends connected to the bottom end of the safety rod and the bottom wall of the movable groove respectively.

6. A pet disease detection device according to claim 1, characterized in that: It also includes a mounting frame for fixing the reagent tube, the mounting frame is arranged at the bottom end of the reagent tube, a hinged plate is arranged on one side of the mounting frame, the hinged plate is connected to a rotating telescopic rod, the rotating telescopic rod is provided with a hinged ball connected to the hinged plate, and the rotating telescopic rod is used to make the mounting frame reciprocate and rotate and rise and fall.

7. A pet disease detection device according to claim 6, characterized in that: It also includes a base, and a guide rod is arranged on the upper end of the base. The guide rod is passed through the mounting frame so that the mounting frame can be reciprocated and rotated and lifted on the guide rod.

8. A pet disease detection device according to claim 7, characterized in that: It also includes a support frame, which is arranged on the base and sleeved on the outer side of the rotating telescopic rod to support and limit the rotating telescopic rod.

9. A pet disease detection device according to claim 6, characterized in that: It also includes a driving component, which includes a pulley and a driving member. The pulley is sleeved on the outside of the rotating telescopic rod, and the output shaft of the driving member is provided with a driving wheel, which is connected to the pulley through a belt.

10. A pet disease detection device according to claim 7, characterized in that: It also includes a spectrometer. The mounting frame is provided with a detection window. The spectrometer is arranged on the base and a detection port of the spectrometer corresponds to the detection window.

Images