Cell culture oscillator

Abstract

The utility model relates to the technical field of stem cell culture equipment, and discloses a cell culture oscillator which comprises a bottom frame, an oscillation assembly is arranged in the bottom frame, a plurality of groups of connecting assemblies are arranged at the bottom of an inner cavity of the bottom frame, and the upper ends of the plurality of groups of connecting assemblies are fixedly connected with an oscillation box; through cooperation of the oscillation assembly, the connecting assembly, the oscillation box, the positioning assemblies and the bottom frame, the oscillation stability of the oscillation box is guaranteed through the oscillation assembly and the connecting pieces, then cell containers can be oscillated conveniently, meanwhile, the multiple cell containers can be fixed conveniently through the multiple positioning assemblies, and therefore oscillation culture of multiple cells is achieved; meanwhile, a cell container can be conveniently put in through the flaring guide plate, and the cell container is prevented from being separated in the oscillation process through the anti-skid bottom, so that the multiplication culture efficiency of cell oscillation culture is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture equipment, in particular to a cell culture oscillator. Background Technique

[0002] Stem cells are a class of cells with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated progeny cells. In biological cell experiments, it is often necessary to culture stem cells. Under static culture conditions, the cells adhere to the bottom bottle wall, and concentration gradients of dissolved oxygen and nutrients will form. Under mild shaking culture conditions, the concentration gradients are eliminated, the dissolved oxygen concentration is increased, which is more conducive to growth. Therefore, when amplifying and culturing stem cells, it is necessary to shake the stem cells. In order to avoid manual shaking, an oscillator is generally used for shaking;

[0003] In the prior art, the Chinese utility model content with the publication number of CN212800393U discloses an oscillator for cell culture, including a support and placement member, a fixed hydraulic press, a drive rod, a movable collar, a flexible bearing member, a multi-component sliding oscillation device, a fixed collar and a rotating component. The fixed end of the fixed hydraulic press is arranged on the support and placement member, one end of the drive rod is arranged on the movable end of the fixed hydraulic press, the movable collar is arranged at the other end of the drive rod, the rotating component is arranged on the support and placement member, the fixed collar is arranged on the rotating component, the fixed collar is arranged below the movable collar, the flexible bearing member is respectively arranged on the movable collar and the fixed collar, and the multi-component sliding oscillation device is slidably arranged on the support and placement member and close to the flexible bearing member. This utility model belongs to the technical field of oscillators, and specifically refers to an oscillator for cell culture that can fix the culture bottle and oscillate the culture bottle in all directions;

[0004] Although the above technical solution can oscillate the cell culture container, this technical solution can only oscillate one group of containers each time, and the oscillation efficiency of cell culture oscillation is relatively low, which in turn affects the cell culture efficiency. Therefore, we need to propose a cell culture oscillator. Content of the Utility Model

[0005] The purpose of the utility model is to provide a cell culture oscillator, which is convenient for fixing multiple cell culture containers, so as to facilitate synchronous oscillation of multiple cell culture containers, improve the oscillation efficiency of cell culture containers, and shorten the cell culture process, so as to solve the problems raised in the above background technique.

[0006] To achieve the above object, the present utility model provides the following technical solutions: A cell culture oscillator, comprising a chassis, an oscillation assembly is installed inside the chassis, a plurality of connecting assemblies are arranged at the bottom of the inner cavity of the chassis, and an oscillation box is fixedly connected to the upper ends of the plurality of connecting assemblies. Connecting holes are formed on both sides of the oscillation box, and rotating seats for rotatably connecting the connecting holes are symmetrically arranged at the upper end of the chassis. A plurality of positioning assemblies are arranged inside the oscillation box, and a cell container is inserted into the inner cavity of each positioning assembly;

[0007] The positioning assembly includes two clamping guard plates. A plurality of telescopic rods are fixedly connected to the opposite sides of the two clamping guard plates, and springs are sleeved on the outer sides of the plurality of telescopic rods. Flaring guide plates are fixedly connected to the upper ends of the two clamping guard plates, and an anti-slip bottom is fixedly connected to the lower surface of the two clamping guard plates.

[0008] Preferably, the lengths of the two clamping guard plates are both smaller than the length of the cell container, and the flaring guide plates are arranged in an inverted conical shape.

[0009] Preferably, three partition plates are fixedly connected to the inner cavity of the oscillation box, and four installation chambers for installing the positioning assemblies are formed by separating the inner cavity of the oscillation box through the three partition plates.

[0010] Preferably, a control switch is arranged on one side of the chassis, the control switch is electrically connected to the oscillation assembly, and the oscillation box is arranged above the oscillation assembly.

[0011] Preferably, the oscillation assembly includes two rotating shafts rotatably connected to both sides of the inner cavity of the chassis. One end of each of the two rotating shafts penetrates through the chassis and is fixedly connected with a sprocket, and the two sprockets are both drivingly connected with a synchronous chain. One end of one of the rotating shafts penetrates through the chassis and is driven by a driving motor.

[0012] Preferably, a plurality of cams are fixedly connected to the outer sides of the two rotating shafts, and the plurality of cams are arranged at equal intervals. The plurality of cams on the two rotating shafts are arranged in point symmetry.

[0013] Preferably, the top of the cam on one of the rotating shafts abuts against one end of the lower surface of the oscillation box, and the lower ends of the plurality of cams on the other rotating shaft abut against the other end of the lower surface of the oscillation box.

[0014] Preferably, each connecting assembly includes two suspension members and a tension spring. The two ends of the tension spring are respectively fixedly connected to the opposite ends of the two suspension members. One of the suspension members is fixedly connected to the bottom of the inner cavity of the chassis, and the other suspension member is fixedly connected to the lower surface of the oscillation shell.

[0015] Compared with the prior art, the beneficial effects of the present utility model are:

[0016] The utility model mainly realizes the oscillation culture of multiple cells by the cooperation among an oscillation component, a connection component, an oscillation box, a positioning component and a chassis. The oscillation stability of the oscillation box is ensured by the oscillation component and the connecting piece, so as to facilitate the oscillation of the cell container. At the same time, multiple groups of positioning components are convenient for fixing multiple cell containers, so as to shorten the cell culture process. Meanwhile, the flared guide plate is convenient for putting the cell container in, and the anti-slip bottom prevents the cell container from detaching during the oscillation process, thereby improving the amplification culture efficiency of cell oscillation culture. Brief Description of the Drawings

[0017] Figure 1 is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 is a schematic diagram of the oscillation component structure of the utility model;

[0019] Figure 3 is a schematic diagram of the oscillation box structure of the utility model;

[0020] Figure 4 is a schematic diagram of the container positioning component structure of the utility model.

[0021] In the figure: 1, chassis; 2, oscillation component; 21, drive motor; 22, rotating shaft; 23, cam; 24, sprocket; 25, synchronous chain; 3, connection component; 31, suspension piece; 32, tension spring; 4, control switch; 5, rotating seat; 6, oscillation box; 7, partition board; 8, installation chamber; 9, positioning component; 91, clamping guard plate; 92, anti-slip bottom; 93, flared guide plate; 94, telescopic rod; 95, spring; 10, cell container; 11, connection hole. Detailed Description of the Preferred Embodiment

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0023] Please refer to Figures 1-4 , the present invention provides a technical solution: a cell culture oscillator, including a chassis 1, an oscillation component 2 is installed inside the chassis 1, multiple groups of connection components 3 are arranged at the bottom of the inner cavity of the chassis 1, and the upper ends of the multiple groups of connection components 3 are fixedly connected with an oscillation box 6. Connection holes 11 are opened on both sides of the oscillation box 6. Rotating seats 5 for rotatably connecting the connection holes 11 are symmetrically arranged at the upper end of the chassis 1. Multiple groups of positioning components 9 are arranged inside the oscillation box 6, and a cell container 10 is inserted into each group of positioning components 9;

[0024] The positioning assembly 9 includes two groups of clamping guard plates 91. On the opposite sides of the two groups of clamping guard plates 91, a plurality of telescopic rods 94 are fixedly connected, and springs 95 are sleeved on the outer sides of the plurality of telescopic rods 94. Flaring guide plates 93 are fixedly connected to the upper ends of the two groups of clamping guard plates 91, and anti-slip bottoms 92 are fixedly connected to the lower surfaces of the two groups of clamping guard plates 91.

[0025] The lengths of the two groups of clamping guard plates 91 are both smaller than the length of the cell container 10. The flaring guide plates 93 are arranged in an inverted conical shape, which is convenient for taking and placing the cell container 10.

[0026] Three partition plates 7 are fixedly connected to the inner cavity of the oscillation box 6. Four installation chambers 8 for installing the positioning assembly 9 are formed in the inner cavity of the oscillation box 6 through the three partition plates 7. The partition plates 7 facilitate dividing the oscillation box 6 into multiple cavities, so as to install multiple positioning assemblies 9 in the cavities, so as to realize the oscillation of multiple cell containers 10 and improve the efficiency of cell culture oscillation.

[0027] A control switch 4 is arranged on one side of the chassis 1. The control switch 4 is electrically connected to the oscillation assembly 2. The oscillation box 6 is arranged above the oscillation assembly 2. The control switch 4 facilitates controlling the start and stop of the oscillation assembly 2, so as to facilitate the cell culture oscillation, and the operation is simple.

[0028] The oscillation assembly 2 includes two groups of rotating shafts 22 rotatably connected to both sides of the inner cavity of the chassis 1. One ends of the two groups of rotating shafts 22 penetrate through the chassis 1 and are fixedly connected with sprockets 24, and the two groups of sprockets 24 are both drivingly connected with a synchronous chain 25. One end of one of the rotating shafts 22 penetrates through the chassis 1 and is driven by a driving motor 21. By driving the rotating shaft 22 by the driving motor 21, the two groups of rotating shafts 22 rotate synchronously under the driving action of the sprockets 24 and the synchronous chain 25, so as to realize the efficient oscillation of the oscillation box 6.

[0029] A plurality of cams 23 are fixedly connected to the outer sides of the two groups of rotating shafts 22, and the plurality of cams 23 are arranged at equal intervals. The plurality of cams 23 on the two groups of rotating shafts 22 are arranged in point symmetry. By the relative arrangement of the cams 23, the cams 23 are tightly abutted against the oscillation box 6, so as to improve the stability of the oscillation box 6 during oscillation and avoid the disorder of the oscillation frequency of the oscillation box 6.

[0030] The top ends of the cams 23 on one of the rotating shafts 22 are abutted against one end of the lower surface of the oscillation box 6, and the lower ends of the plurality of cams 23 on the other rotating shaft 22 are abutted against the other end of the lower surface of the oscillation box 6. By the cams 23 abutting against the oscillation box 6, the oscillation box 6 swings around the connection hole 11 as the axis, and then the cells in the cell container 10 are oscillated, so as to improve the cell culture efficiency.

[0031] Each set of connecting components 3 includes two sets of suspension members 31 and a tension spring 32. The two ends of the tension spring 32 are respectively fixedly connected to the opposite ends of the two sets of suspension members 31. One set of suspension members 31 is fixedly connected to the bottom of the inner cavity of the chassis 1, and the other set of suspension members 31 is fixedly connected to the lower surface of the oscillation housing. The tension spring 32 makes the lower surface of the oscillation box 6 abut against the cams 23 on the two sets of rotating shafts 22, so as to ensure the stable contact area between the cam 23 and the oscillation box 6 and ensure the stable oscillation frequency.

[0032] During use, the cell container 10 containing cells is aligned with the flared guide plates 93 of the two sets of clamping guard plates 91, and then a downward pressure is applied to the cell container 10, so that the cell container 10 is inserted into the two sets of clamping guard plates 91 along the flared guide plates 93. The two sets of clamping guard plates 91 compress the spring 95, and then adjust the distance between the two sets of clamping guard plates 91, so that the clamping guard plates 91 clamp and fix the cell containers 10 with different capacities, and improve the friction with the cell container 10 through the anti-slip bottom 92, thereby improving the stability of clamping the cell container 10. The driving motor 21 is controlled by the control switch 4 to drive the rotating shaft 22 to rotate. The two sets of rotating shafts 22 rotate synchronously under the linkage of the sprockets 24 and the synchronous chain 25, and then the cams 23 on the two sets of rotating shafts 22 abut against the oscillation box 6, so that the oscillation box 6 swings within a certain range with the connecting hole 11 as the axis, thereby realizing the oscillation of the oscillation box 6, accelerating the efficiency of cell culture, and under the action of the connecting component 3, ensuring that the bottom of the oscillation box 6 always abuts against the cam 23, thereby improving the oscillation efficiency.

[0033] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture oscillator, characterized in that include: A base frame (1), wherein an oscillating assembly (2) is installed inside the base frame (1); A plurality of connection components (3) are arranged at the bottom of the inner cavity of the base frame (1), and an oscillation box (6) is fixedly connected to the upper ends of the plurality of connection components (3), and connection holes (11) are provided on both sides of the oscillation box (6); A rotating seat (5) symmetrically arranged at the upper end of the base frame (1) for rotationally connecting the connecting hole (11), the inner cavity of the oscillation box (6) is provided with a plurality of groups of positioning components (9), and the inner cavity of each group of positioning components (9) is plugged with a cell container (10); The positioning assembly (9) comprises two groups of clamping guard plates (91), and the opposite sides of the two groups of clamping guard plates (91) are fixedly connected to multiple groups of telescopic rods (94), and the outer sides of the multiple groups of telescopic rods (94) are sleeved with springs (95), the upper ends of the two groups of clamping guard plates (91) are fixedly connected to flaring guide plates (93), and the lower surfaces of the two groups of clamping guard plates (91) are fixedly connected to anti-slip bottoms (92).

2. A cell culture oscillator according to claim 1, characterized in that: The lengths of the two groups of clamping guard plates (91) are both shorter than the length of the cell container (10), and the expansion guide plate (93) is arranged in an inverted cone shape.

3. A cell culture oscillator according to claim 2, characterized in that: The inner cavity of the oscillation box (6) is fixedly connected to three groups of partitions (7), and the inner cavity of the oscillation box (6) is isolated by the three groups of partitions (7) to form four groups of installation chambers (8) for installing positioning components (9).

4. A cell culture oscillator according to claim 3, characterized in that: A control switch (4) is provided on one side of the base frame (1), the control switch (4) is electrically connected to the oscillation component (2), and the oscillation box (6) is arranged above the oscillation component (2).

5. A cell culture oscillator according to claim 4, characterized in that: The oscillating assembly (2) comprises two groups of rotating shafts (22) rotatably connected to both sides of the inner cavity of the base frame (1), one end of each of the two groups of rotating shafts (22) passes through the base frame (1) and is fixedly connected to a sprocket (24), and both groups of sprockets (24) are drivingly connected to a synchronous chain (25), wherein one end of each group of rotating shafts (22) passes through the base frame (1) and is driven by a driving motor (21).

6. A cell culture oscillator according to claim 5, characterized in that: Multiple groups of cams (23) are fixedly connected to the outer sides of the two groups of rotating shafts (22), and the multiple groups of cams (23) are arranged at equal intervals. The multiple groups of cams (23) on the two groups of rotating shafts (22) are arranged in point symmetry.

7. A cell culture oscillator according to claim 6, characterized in that: The top ends of the cams (23) on one group of the rotating shafts (22) abut against one end of the lower surface of the oscillation box (6), and the lower ends of the multiple groups of cams (23) on another group of the rotating shafts (22) abut against the other end of the lower surface of the oscillation box (6).

8. A cell culture oscillator according to claim 7, characterized in that: Each group of the connecting components (3) comprises two groups of suspension members (31) and tension springs (32), the two ends of the tension springs (32) being fixedly connected to opposite ends of the two groups of suspension members (31), one group of the suspension members (31) being fixedly connected to the bottom of the inner cavity of the base frame (1), and the other group of the suspension members (31) being fixedly connected to the lower surface of the oscillation shell.

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