Feeding device suitable for diaphorina citri feeding method experiment

Abstract

The utility model discloses a feeding device suitable for diaphorina citri feeding method experiments, which relates to the technical field of auxiliary tools for bioengineering experiments and comprises a shading box, a supporting plate detachably connected to the inner wall of the middle of the shading box, an LED lamp panel mounted on the inner top surface of the shading box, a plurality of circular openings formed in the supporting plate, transparent tubes placed in the circular openings, and the transparent tubes are connected with the shading box. A sealing film is arranged on the top face of the transparent pipe, a sealing cover is detachably connected to the bottom of the transparent pipe, a shading plate is detachably connected to any side of the shading box, and the top of the shading plate is flush with the bottom of the supporting plate. Through the arrangement of the transparent tube, the sealing film and the detachable sealing cover, the diaphorina citri is cultured in the diaphorina citri culture box, feed and bacterial liquid (or liquid medicine) in the sealing film are taken through the mouthpart, directional and quantitative selection is achieved, pollution and waste of the feed and the bacterial liquid (or liquid medicine) cannot be caused, meanwhile, phenotype changes of the diaphorina citri can be observed conveniently, and the diaphorina citri culture box is convenient to use. The device is reasonable in structure, easy to operate and suitable for being applied to research of diaphorina citri pharmacology and intestinal flora.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering experiment auxiliary tools, in particular to a feeding device suitable for a citrus psyllid feeding method experiment. Background Art

[0002] Citrus fruits (tangerines, oranges, grapefruits, mandarins, etc.) are the world's largest fruit category. Citrus Huanglongbing is a major bacterial disease that is difficult to cure in the citrus industry, and citrus psyllids are important insect vectors for the spread of Huanglongbing bacteria.

[0003] The prior art lacks research on the function of the intestinal flora of citrus psyllid. Based on the piercing-sucking mouthparts of citrus psyllid and mature artificial feed, a feeding device that can be used for citrus psyllid feeding experiments in the laboratory is provided, which facilitates the research and prevention of the gene function of citrus psyllid. Utility Model Content

[0004] To achieve the above-mentioned purpose, the utility model provides the following solution: The utility model provides a feeding device suitable for citrus psyllid feeding method experiments, including a light-shielding box, the middle inner wall of the light-shielding box is detachably connected to a support plate, an LED light board is installed on the top surface of the light-shielding box, a plurality of circular openings are provided on the support plate, transparent tubes are placed in the circular openings, a sealing film is provided on the top surface of the transparent tube, a sealing cover is detachably connected to the bottom of the transparent tube, and a light-shielding plate is detachably connected to either side of the light-shielding box, and the top of the light-shielding plate is flush with the bottom of the support plate.

[0005] Preferably, the sealing film comprises an upper sealing film and a lower sealing film, and a gap is provided between the upper sealing film and the lower sealing film.

[0006] Preferably, the sealing cover is provided with a plurality of air holes.

[0007] Preferably, a shading ring is detachably connected to the top side wall of the transparent tube, and the shading ring is located above the supporting plate.

[0008] Preferably, a transparent plate is fixedly connected to the side wall of the shading box, and the shading plate is located inside the transparent plate and is slidably connected to the transparent plate.

[0009] Preferably, a plurality of shading covers are detachably connected to the support plate, and the plurality of shading covers are arranged in one-to-one correspondence with the plurality of circular openings.

[0010] Preferably, a rear plate pull ring is fixedly connected to a side of the light-shielding box away from the transparent plate.

[0011] Preferably, slide rails are symmetrically arranged in the light shielding box, and two sides of the support plate are slidably connected to the slide rails.

[0012] The utility model discloses the following technical effects: the material of the transparent tube can be preferably plastic or glass, and the transparent tube can be light-transmissive; there are 25 circular openings on the supporting plate, and the transparent tube is placed in the circular openings to keep the transparent tube stable; the lower end of the transparent tube is buckled with a sealing cap, and the citrus psyllid is cultured in the transparent tube, the top of the transparent tube is light-transmissive, and the LED light board is turned on (the light of the LED light board is adjustable, and the citrus psyllid has significant differences in the tendency to light of different wavelengths, among which ultraviolet light, blue light, and green light are the most attractive to it, but ultraviolet light is the most attractive to it. The light-driven characteristic of insects is used to make the insects move to the top of the transparent tube and reach the sealing membrane. Artificial feed and bacterial solution are added to the sealing membrane. The citrus psyllid pierces the sealing membrane with its mouthparts to eat the feed and the corresponding bacterial solution, so as to achieve the purpose of feeding bacterial solution culture. During observation, the shading plate is opened, and the changes in the phenotype of the citrus psyllid, such as mortality and deformity rate, are observed through the transparent tube, and the data are recorded to obtain the technology of the pharmacology and intestinal flora research of the citrus psyllid. The utility model is provided with a transparent tube, a sealing membrane and a detachable sealing cover, and the citrus psyllid is cultured therein, and the feed and bacterial solution (or medicinal solution) in the sealing membrane are eaten through the mouthparts, and the selection is directional and quantitative, which will not cause pollution and waste of the feed and bacterial solution (or medicinal solution), and it is also convenient for observing the phenotypic changes of the citrus psyllid. It has a reasonable structure and is simple to operate, and is suitable for the research of the pharmacology and intestinal flora of the citrus psyllid. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0014] Figure 1 It is a schematic diagram of the structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the structure of the transparent tube of the utility model;

[0016] Figure 3 This is a schematic diagram of the structure of the light shielding cover of the utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the sealing film of the utility model;

[0018] In the figure: 1, air hole; 2, sealing cover; 3, transparent tube; 4, shading cover; 5, shading ring; 6, supporting plate; 7, rear plate pull ring; 8, transparent plate; 9, shading plate; 10, LED light board; 205, upper sealing film; 206, lower sealing film; 207, gap. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0021] Reference Figure 1-Figure 4 As shown, this embodiment provides a feeding device suitable for a citrus psyllid feeding method experiment, including a light-shielding box, a support plate 6 is detachably connected to the inner wall in the middle of the light-shielding box, an LED light board 10 is installed on the top surface of the light-shielding box, a plurality of circular openings are provided on the support plate 6, a transparent tube 3 is placed in the circular opening, a sealing film is provided on the top surface of the transparent tube 3, a sealing cover 2 is detachably connected to the bottom of the transparent tube 3, and a light shielding plate 9 is detachably connected to either side of the light-shielding box, and the top of the light shielding plate 9 is flush with the bottom of the support plate 6.

[0022] The LED light board 10 is located on the upper part of the light shielding box, and the distance from the light shielding box is 10 cm. In order to ensure the phototaxis of insects, a gap 207 is left between the LED light and the light shielding box; the material of the transparent tube 3 can be preferably plastic or glass, and the transparent tube 3 can transmit light; the number of circular openings on the supporting plate 6 is 25, and the transparent tube 3 is placed in the circular opening to keep the transparent tube 3 stable; the sealing cover 2 is buckled at the lower end of the transparent tube 3, and the citrus psyllid is cultured in the transparent tube 3. The top of the transparent tube 3 is transparent, and the LED light board 10 is turned on (the light of the LED light board 10 is adjustable, and the citrus psyllid has a significant sensitivity to light of different wavelengths). The insects are attracted to different light sources, such as ultraviolet light, blue light, and green light, but ultraviolet light will kill the bacteria in the bacterial solution between the sealing films, so blue light and green light are used. The light-driven characteristics of insects are used to make the insects move to the top of the transparent tube 3 and reach the sealing film. Artificial feed and bacterial solution are added to the sealing film. The citrus psyllid pierces the sealing film with its mouthparts and eats the feed and the corresponding bacterial solution, so as to achieve the purpose of feeding bacterial solution culture. During observation, the shading plate 9 is opened, and the changes in the phenotypic type of the citrus psyllid, such as mortality and deformity rate, are observed through the transparent tube 3, and the data are recorded to obtain the technology of citrus psyllid pharmacology and intestinal flora research. The utility model is provided with a transparent tube 3, a sealing film and a detachable sealing cover 2, wherein the citrus psyllid is cultured therein, and the feed and bacterial solution (or medicinal solution) in the sealing film are fed by the mouthparts, and the feed and bacterial solution (or medicinal solution) are selected in a directional and quantitative manner, and the pollution and waste of the feed and bacterial solution (or medicinal solution) are not caused. Meanwhile, the utility model is convenient for observing the phenotypic changes of the citrus psyllid, has a reasonable structure and is simple to operate, and is suitable for the research on the pharmacology of the citrus psyllid and the intestinal flora.

[0023] In a further optimized solution, the sealing film includes an upper sealing film 205 and a lower sealing film 206, and a gap 207 is provided between the upper sealing film 205 and the lower sealing film 206. Both the upper sealing film 205 and the lower sealing film 206 are Parafilm sealing films, and the two layers of film are used to fix the artificial feed and the bacterial solution in the gap 207, which is convenient for the piercing and sucking mouthparts of the citrus psyllid to pierce the sealing film to feed, and prevents the contamination of the feed and the bacterial solution, wherein the bacterial solution is extracted and purified from the intestinal flora in the body of the citrus psyllid, including the bacterial solution made from the intestinal bacteria of the citrus psyllid related to the nutrition and metabolism, immunity and defense, growth and development, transmission of Huanglongbing bacteria, behavioral habits, etc. of the citrus psyllid.

[0024] In a further optimized solution, a plurality of air holes 1 are provided on the sealing cover 2. The air holes 1 on the sealing cover 2 can maintain the oxygen content in the device, and the sealing cover 2 is fixed by the lower cover ring, which is made of a relatively soft material and has an interference fit, and the lower cover ring can be tightly buckled on the lower end of the transparent tube 3 through a slight deformation.

[0025] Furthermore, the distance between the bottom of the light shielding box and the lower cover ring is 50-100 mm. In order to ensure the oxygen supply in the transparent tube 3, a gap is left between the light shielding box and the lower cover ring.

[0026] In a further optimized solution, the top side wall of the transparent tube 3 is detachably connected with a light shielding ring 5, which is located above the support plate 6. A closable button is provided at the middle end of the light shielding ring 5, which can hold the plastic tube to keep it stable and convenient to take. It should be fastened when not in use to avoid affecting the phototaxis of insects.

[0027] Further optimization scheme, the side wall of the light shielding box is fixed with a transparent plate 8, and the light shielding plate 9 is located inside the transparent plate 8 and is slidably connected to the transparent plate 8. In order to facilitate observation, the front wall of the light shielding box is changed to a transparent plate 8, and the transparent plate 8 is a double-layer transparent plastic plate. The light shielding plate 9 is located between the double-layer transparent plastic plates and can slide. The situation in the lower part of the transparent tube 3 can be observed by pulling open the light shielding plate 9. When not observing, the black light shielding plate 9 is closed to avoid affecting the phototaxis of insects and affecting the feeding effect.

[0028] In a further optimized solution, a plurality of shading covers 4 are detachably connected to the support plate 6, and the plurality of shading covers 4 are arranged one by one corresponding to the plurality of circular openings. When not in use, the shading covers 4 are buckled on the circular openings, and when in use, the shading covers 4 are removed and the transparent tubes 3 are placed.

[0029] Further optimizing scheme, the side of the light shielding box away from the transparent plate 8 is fixed with a rear plate pull ring 7. Pulling the rear plate pull ring 7 can open the rear plate of the light shielding box, thereby facilitating the placement and removal of the transparent tube 3.

[0030] Further optimizing the scheme, slide rails are symmetrically arranged in the light shielding box, and the two sides of the support plate 6 are slidably connected with the slide rails. The slide rails support the support plate 6 and facilitate the installation and removal of the support plate 6.

[0031] Working principle:

[0032] When in use, the back plate of the light-shielding box is opened, the supporting plate 6 is pulled out, the light-shielding cover 4 is taken out, the transparent tube 3 is put into the light-shielding box, and the light-shielding ring 5 (with a button on it) is put on the upper part of the transparent tube 3 to put it into the light-shielding box. The button can stabilize the plastic tube and is convenient to take out, but it should be fastened when not in use to avoid affecting the phototaxis of insects. The transparent tube 3 is sealed with a lower sealing film 206 that is pulled toward both ends to make it thinner, and then a mixture of artificial feed and bacterial liquid is injected, and then the upper sealing film 205 that is pulled toward both ends to make it thinner is used to seal; plug in the power supply, turn on the LED light to adjust the light that the citrus psyllid has a tendency to, close the back plate, so that the citrus psyllid can gather at the top of the transparent tube 3 to absorb nutrients, and the upper sealing film 205 and the lower sealing film 206 are materials that can ensure that the mouthparts of insects can penetrate without the liquid wrapped inside flowing out; after a certain period of time, the light-shielding plate 9 of the front plate of the light-shielding box can be pulled out for observation.

[0033] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0034] The embodiments described above are only descriptions of the preferred methods of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the claims of the present invention.

Claims

1. A feeding device suitable for a feeding experiment of citrus psyllid, characterized in that: The light shielding box comprises a light shielding box, wherein the inner wall in the middle of the light shielding box is detachably connected to a support plate (6), an LED light panel (10) is installed on the top surface of the light shielding box, a plurality of circular openings are provided on the support plate (6), a transparent tube (3) is placed in the circular opening, a sealing film is provided on the top surface of the transparent tube (3), a sealing cover (2) is detachably connected to the bottom of the transparent tube (3), and a light shielding plate (9) is detachably connected to either side of the light shielding box, and the top of the light shielding plate (9) is flush with the bottom of the support plate (6).

2. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: The sealing film comprises an upper sealing film (205) and a lower sealing film (206), and a gap (207) is provided between the upper sealing film (205) and the lower sealing film (206).

3. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: The sealing cover (2) is provided with a plurality of air holes (1).

4. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: The top side wall of the transparent tube (3) is detachably connected to a light shielding ring (5), and the light shielding ring (5) is located above the supporting plate (6).

5. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: A transparent plate (8) is fixedly connected to the side wall of the light shielding box, and the light shielding plate (9) is located inside the transparent plate (8) and is slidably connected to the transparent plate (8).

6. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: A plurality of light shielding covers (4) are detachably connected to the support plate (6), and the plurality of light shielding covers (4) are arranged in one-to-one correspondence with the plurality of circular openings.

7. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 5, characterized in that: A rear plate pull ring (7) is fixedly connected to a side of the light shielding box away from the transparent plate (8).

8. The feeding device suitable for the feeding method experiment of citrus psyllid according to claim 1, characterized in that: Slide rails are symmetrically arranged in the light shielding box, and two sides of the support plate (6) are slidably connected to the slide rails.

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