High-sealing-performance fermentation device for polyglutamic acid

Abstract

The utility model discloses a high-sealing-performance fermentation device for polyglutamic acid, and belongs to the field of polyglutamic acid fermentation. The fermentation tank mainly comprises a fermentation tank body, a detachable sealing cover is arranged on the fermentation tank body, a plurality of evenly-distributed locking mechanisms are arranged between the fermentation tank body and the sealing cover, an annular protrusion with the section of a trapezoidal structure is fixedly connected to the middle of the upper end face of the fermentation tank body, and a first groove is formed in the outer side wall of the upper portion of the fermentation tank body. A first sealing ring is arranged in the first groove, a first annular groove corresponding to the annular protrusion is formed in the sealing cover, the bottoms of the two sides of the section of the first annular groove are provided with inclined faces inclining upwards respectively, an inverted-V-shaped sealing ring capable of being tightly matched with the annular protrusion is arranged in the first annular groove, and the periphery of the sealing cover extends downwards to form an outer boss. And the inner side wall of the outer boss is tightly matched with the first sealing ring. Under the condition that the sealing cover is quickly assembled and disassembled, high sealing performance between the sealing cover and the fermentation tank body is improved, and external pollution is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of polyglutamic acid fermentation, and in particular relates to a highly sealed polyglutamic acid fermentation device. Background Art

[0002] The fermentation process of polyglutamic acid mainly includes seed culture, fermentation culture, fermentation liquid treatment and polyglutamic acid extraction. Among them, fermentation culture is the core link of the whole process, and various fermentation conditions need to be strictly controlled to ensure the high yield and high quality of polyglutamic acid.

[0003] The Chinese patent with the authorization announcement number "CN218202754U" discloses a γ-polyglutamic acid fermentation device, including a fermentation tank, a stirring rod is arranged inside the fermentation tank, a stirring paddle and a defoaming paddle are installed on the axial surface of the stirring rod, a coupling box is installed on the top surface of the fermentation tank, a motor is installed on one side of the coupling box, a defoaming agent pipe is fixedly connected to the outer surface of the fermentation tank, one end of the defoaming agent pipe is connected to a booster pump, one end of the booster pump is installed with a solenoid valve, and one end of the solenoid valve is connected to a defoaming agent tank. The fermentation device is installed with an oxygen pump, and oxygen is transported into the oxygen supply pipe by opening a one-way valve and starting the oxygen pump. The oxygen then enters the shunt pipe, and the shunt pipe transports the oxygen to each aeration head, and the aeration head transports the oxygen into the fermentation solution, thereby increasing the dissolved oxygen rate of the γ-polyglutamic acid fermentation liquid and improving the fermentation quality of the γ-polyglutamic acid.

[0004] Although the above patent solves the problem of dissolved oxygen rate of fermentation liquid, the following problems still exist during use: 1. Before fermentation, the inside of the tank needs to be thoroughly cleaned and disinfected. There are dead corners in the tank, which makes cleaning difficult and the fermentation quality difficult to guarantee; 2. The sealing performance of the device is poor, and it is impossible to completely isolate the wall from external pollution, and the fermentation liquid leaks and the inhaled gas diverges, which seriously affects the quality of polyglutamic acid fermentation; 3. The dissolved oxygen rate of the stirring mechanism is not good.

[0005] In view of the above technical problems, this application proposes a solution. Utility Model Content

[0006] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a highly sealed polyglutamic acid fermentation device, which greatly improves the high sealing between the sealing cover and the fermentation tank body while ensuring rapid assembly and disassembly of the sealing cover, thereby reducing external pollution.

[0007] The highly sealed polyglutamic acid fermentation device comprises a fermentation tank body, a plurality of supporting legs are fixedly connected to the bottom of the fermentation tank body, a detachable sealing cover is provided on the fermentation tank body, a plurality of evenly distributed locking mechanisms are provided between the fermentation tank body and the sealing cover, an annular protrusion with a trapezoidal cross-section is fixedly connected to the middle of the upper end surface of the fermentation tank body, a first groove is provided on the upper outer wall of the fermentation tank body, a first sealing ring is provided in the first groove, a first annular groove corresponding to the annular protrusion is provided in the sealing cover, upwardly inclined inclined surfaces are respectively provided on the bottom of both sides of the cross section of the first annular groove, an inverted V-shaped sealing ring that can closely match the annular protrusion is provided in the first annular groove, an outer boss extends downward from the outer periphery of the sealing cover, and the inner side wall of the outer boss closely matches the first sealing ring.

[0008] Preferably, the side of the first sealing ring close to the outer boss is an arc-shaped structure, and a stopper is provided at the bottom of the first groove for preventing the first sealing ring from falling out.

[0009] Preferably, the locking mechanism includes a fixed seat, which is fixedly connected to the outer wall of the fermentation tank body, and a pin is rotatably connected to the fixed seat, a threaded rod is fixedly connected to the pin, and a locking nut is threadedly connected to the threaded rod; an annular clamping plate is fixedly connected to the outer side of the external boss, and a plurality of clamping grooves are provided on the annular clamping plate for fixing the locking mechanism.

[0010] Preferably, the outer side of the clamping groove passes through the annular clamping plate, and the locking nut abuts against the upper end surface of the annular clamping plate.

[0011] Preferably, a reduction motor is fixedly installed on the sealing cover, the output shaft of the reduction motor passes through the sealing cover and is sealed and rotatably connected to the sealing cover, the output shaft of the reduction motor is fixedly connected to a stirring and lifting mechanism, an air inlet pipe passes through the sealing cover, the bottom of the air inlet pipe extends to the lower part of the fermentation tank body, and a discharge port is provided at the bottom of the fermentation tank body.

[0012] Preferably, the stirring and lifting mechanism includes a stirring rod, the upper end of which is fixedly connected to the output shaft of the reduction motor through a coupling, the stirring rod is provided with a detachable spiral lifting blade, the other end of the stirring rod is fixedly connected to a large turntable, and the outer circumference of the large turntable is fixedly connected to a plurality of evenly distributed stirring blades.

[0013] Preferably, the diameter of the large turntable is larger than the diameter of the spiral lifting blades.

[0014] Preferably, the sealing cover is also provided with a feeding mechanism, the feeding mechanism includes a feeding pipe and a supply pipe, the feeding pipe is fixedly connected to the sealing cover, a lower flange is sealingly and fixedly connected to the feeding pipe, the lower flange is connected to the upper flange by bolts, a second annular groove is opened on the upper flange, a second sealing ring is arranged in the second annular groove, the upper and lower end surfaces of the outer circumference of the second sealing ring are respectively provided with protrusions, an annular clamping block corresponding to the second annular groove is provided on the lower flange, and the annular clamping block is tightly matched with the second sealing ring.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. A detachable sealing cover is added, so that when cleaning the inside of the fermenter body, the fermenter body can be directly opened for cleaning, avoiding dead corners for cleaning, which provides a guarantee for the subsequent polyglutamic acid fermentation; and while ensuring the quick loading and unloading of the sealing cover, the high sealing between the sealing cover and the fermenter body is greatly improved, reducing external pollution.

[0017] 2. An annular protrusion, a first annular groove and an inverted V-shaped sealing ring are added between the sealing cover and the fermentation tank body. The container in the inverted V-shaped sealing ring is slightly smaller than the annular protrusion, which facilitates the annular protrusion and the inverted V-shaped sealing ring to achieve a high sealing combination. Inclined surfaces are added on both sides of the bottom of the first annular groove, and the inclined surfaces are used to clamp the inverted V-shaped sealing ring to prevent it from falling off; secondly, a first groove and a first sealing ring are added to the outer wall of the fermentation tank body. The outer side of the first sealing ring is an arc structure, which is conducive to the downward pressure of the sealing cover. At the same time, the first sealing ring plays a role of secondary sealing.

[0018] 3. The stirring and lifting mechanism is lifted by the cooperation of the spiral lifting blades and the stirring blades. The stirring blades are located at the bottom of the fermentation tank body and play a role in mixing and stirring. The spiral lifting blades play a role in lifting. During the stirring process, the bottom mixed liquid is lifted upward, accelerating the circulation of the liquid inside the fermentation tank body, playing a role of full mixing, and greatly improving the dissolved oxygen rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2 for Figure 1 A partial enlarged view of part A;

[0021] Figure 3 It is the front view of the utility model;

[0022] Figure 4 for Figure 3 Middle AA section view;

[0023] Figure 5 for Figure 4 A partial enlarged view of part B;

[0024] Figure 6 for Figure 4 A partial enlarged view of part C in the middle;

[0025] Figure 7 is a schematic diagram of the structure of the second sealing ring;

[0026] Figure 8 It is a schematic diagram of the structure of the sealing cover when viewed from above;

[0027] Fig. 9 It is a structural schematic diagram of the stirring and lifting mechanism.

[0028] In the figure, 1. fermentation tank body; 101. annular protrusion; 102. first sealing ring; 103. baffle; 2. supporting leg; 3. discharge port; 4. sealing cover; 401. inverted V-shaped sealing ring; 4011. inclined surface; 402. slot; 403. outer boss; 5. locking mechanism; 501. fixing seat; 502. threaded rod; 503. pin; 504. locking nut; 6. feeding mechanism; 601. upper flange; 602. lower flange; 603. second sealing ring; 604. protrusion; 7. reduction motor; 8. air inlet pipe; 9. stirring and lifting mechanism; 901. stirring rod; 902. spiral lifting blade; 903. large turntable; 904. stirring blade. DETAILED DESCRIPTION

[0029] The utility model is further described below in conjunction with the accompanying drawings:

[0030] The directional nouns involved in the detailed description paragraphs are only for the convenience of those skilled in the art to understand the technical solutions described in this application according to the visual orientation shown in the accompanying drawings. Unless otherwise clearly specified and limited, the terms "setting", "installation", "connection", etc. should be understood in a broad sense. For ordinary technicians in this field, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0031] like Figures 1 to 9 As shown, a highly sealed polyglutamic acid fermentation device comprises a fermentation tank body 1, a plurality of supporting legs 2 are fixedly connected to the bottom of the fermentation tank body 1, and a fixing plate at the bottom of the supporting legs 2 can be fixed to the ground by bolts, so that the supporting legs 2 play a role in supporting and fixing the fermentation tank body 1, and a detachable sealing cover 4 is provided on the fermentation tank body 1. When cleaning the inside of the fermentation tank body 1, the fermentation tank body 1 can be directly opened and cleaned through the detachable sealing cover 4, so as to avoid the generation of cleaning dead corners, thereby providing a guarantee for the subsequent polyglutamic acid fermentation.

[0032] A plurality of evenly distributed locking mechanisms 5 are provided between the fermentation tank body 1 and the sealing cover 4, and the locking mechanisms 5 facilitate quick installation and removal of the sealing cover 4. Figure 2As shown, the locking mechanism 5 includes two fixing seats 501, and there is a certain distance between the two fixing seats 501. The fixing seats 501 are fixedly connected to the outer side wall of the fermentation tank body 1. A pin shaft 503 is rotatably connected between the two fixing seats 501, and a threaded rod 502 is fixedly connected to the pin shaft 503, and a locking nut 504 is threadedly connected to the threaded rod 502; an outer boss 403 extends downward from the outer periphery of the sealing cover 4, and an annular clamping plate is fixedly connected to the outer side of the outer boss 403, and a plurality of clamping grooves 402 for fixing the locking mechanism 5 are provided on the annular clamping plate, and the outer side of the clamping groove 402 passes through the annular clamping plate. When in use, the pin shaft 503 is rotated to make the threaded rod 502 snap into the clamping groove 402, and the locking nut 504 is tightened to make the locking nut 504 abut against the upper end surface of the annular clamping plate, so that the sealing cover 4 can be locked and fixed by the locking nut 504.

[0033] Under the condition of ensuring the quick installation and removal of the sealing cover 4, in order to improve the sealing performance between the sealing cover 4 and the fermentation tank body 1, as shown in FIG. Figure 5 As shown, an annular protrusion 101 with a trapezoidal cross-section is fixedly connected to the middle part of the upper end surface of the fermentation tank body 1, and a first annular groove corresponding to the annular protrusion 101 is opened in the sealing cover 4, and the cross-section of the first annular groove is a trapezoidal structure larger than the annular protrusion 101, and the bottoms on both sides of the cross-section of the first annular groove are respectively provided with inclined surfaces 4011 inclined upward, and an inverted V-shaped sealing ring 401 that can closely match the annular protrusion 101 is provided in the first annular groove, and the container in the inverted V-shaped sealing ring 401 is slightly smaller than the annular protrusion 101, which is convenient for the annular protrusion 101 to achieve a high sealing combination with the inverted V-shaped sealing ring 401, and the inclined surface 4011 is used to clamp the inverted V-shaped sealing ring 401 to prevent it from falling off.

[0034] A first groove is provided on the outer side wall of the upper part of the fermentation tank body 1, and a first sealing ring 102 is provided in the first groove. An outer boss 403 extends downward from the outer periphery of the sealing cover 4, and the inner side wall of the outer boss 403 is closely matched with the first sealing ring 102. The first sealing ring 102 has an arc-shaped structure on one side close to the outer boss 403, which is conducive to pressing down when the sealing cover 4 is covered, and at the same time, it can better fit closely with the outer boss 403, playing a role of secondary sealing. A stopper 103 is provided at the bottom of the first groove to prevent the first sealing ring 102 from falling out. The stopper 103 plays a limiting role to prevent the first sealing ring 102 from shifting when the sealing cover 4 is installed.

[0035] like Figure 3 and Figure 4As shown, a reduction motor 7 is fixedly installed on the sealing cover 4, and the output shaft of the reduction motor 7 passes through the sealing cover 4 and is sealed and rotatably connected to the sealing cover 4. The output shaft of the reduction motor 7 is fixedly connected to a stirring and lifting mechanism 9. An air inlet pipe 8 passes through the sealing cover 4, and the bottom of the air inlet pipe 8 extends to the lower part of the fermentation tank body 1. A discharge port 3 is provided at the bottom of the fermentation tank body 1. The air inlet pipe 8 is connected to the clean air supply device for continuously supplying oxygen to the fermentation tank body 1, and a discharge valve is installed at the discharge port 3.

[0036] like Fig. 9 As shown, the stirring and lifting mechanism 9 includes a stirring rod 901, the upper end of which is fixedly connected to the output shaft of the reduction motor 7 through a coupling, and a detachable spiral lifting blade 902 is provided on the stirring rod 901, and the spiral lifting blade 902 is fixedly connected to the stirring rod 901 through a fixing rod and a sleeve, and a large turntable 903 is fixedly connected to the bottom of the stirring rod 901, and the diameter of the large turntable 903 is larger than the outer diameter of the spiral lifting blade 902, and a plurality of uniformly distributed stirring blades 904 are fixedly connected to the outer circumference of the large turntable 903, and the stirring blade 904 is inclined. When in use, it cooperates with the spiral lifting blade 902, and the stirring blade 904 is located at the bottom of the fermentation tank body 1, and plays a role in mixing and stirring; the spiral lifting blade 902 rotates synchronously with the stirring blade 904, and plays a role in lifting. During the stirring process, the bottom mixed liquid is lifted upward, and the circulation of the liquid inside the fermentation tank body 1 is accelerated, and the role of full mixing is played, thereby greatly improving the dissolved oxygen rate.

[0037] To further improve the sealing of the feed and prevent external contamination, such as Figure 4 , Figure 6 and Figure 7 As shown, the sealing cover 4 is also provided with a feeding mechanism 6, which includes a feeding pipe and a supply pipe, wherein the feeding pipe is sealed and fixedly connected to the sealing cover 4, a lower flange 602 is sealed and fixedly connected to the feeding pipe, an upper flange 601 is sealed and fixedly connected to the feeding pipe, the lower flange 602 is connected to the upper flange 601 by bolts, so as to connect the feeding pipe and the supply pipe, a second annular groove is opened on the upper flange 601, a second sealing ring 603 is arranged in the second annular groove, and the upper and lower end surfaces of the outer circumference of the second sealing ring 603 are respectively provided with protrusions 604, an annular clamping block corresponding to the second annular groove is provided on the lower flange 602, the annular clamping block is tightly matched with the second sealing ring 603, and in the process of squeezing the second sealing ring 603 by the annular clamping block, the protrusion 604 is deformed, and is highly sealed with the annular clamping block and the inner end surface of the second annular groove, thereby greatly improving the high sealing performance of the equipment.

[0038] When the utility model is used, the inside of the fermentation tank body 1 is first cleaned. When cleaning, the locking nut 504 is first loosened, and the threaded rod 502 is rotated to the side away from the fermentation tank body 1, so that the threaded rod 502 is rotated out of the slot 402. At this time, the sealing cover 4 can be opened to thoroughly clean the inside of the fermentation tank body 1. Then, the sealing cover 4 is covered on the fermentation tank body 1, and the annular protrusion 101 on the fermentation tank body 1 is inserted into the inverted V-shaped sealing ring 401 to achieve a primary seal; at the same time, the first sealing ring 102 is tightly fitted with the outer boss 403 of the sealing cover 4 to achieve a secondary seal. Finally, the locking nut 504 on the locking mechanism 5 is fixed to the annular clamping plate of the sealing cover 4 to complete the sealing installation of the sealing cover 4. At this time, high-temperature steam is passed into the fermentation tank body 1 through the steam pipe, so that the fermentation tank body 1 is sterilized. After the sterilization is completed, when the temperature in the tank reaches the standard, the material and strains can be fed into the fermentation tank body 1 to carry out the fermentation operation.

[0039] Finally, although this specification is described according to implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A highly airtight polyglutamic acid fermentation device, comprising a fermentation tank body (1), a plurality of supporting legs (2) being fixedly connected to the bottom of the fermentation tank body (1), and a detachable sealing cover (4) being provided on the fermentation tank body (1), characterized in that: A plurality of evenly distributed locking mechanisms (5) are provided between the fermentation tank body (1) and the sealing cover (4); an annular protrusion (101) having a trapezoidal cross-section is fixedly connected to the middle of the upper end surface of the fermentation tank body (1); a first groove is provided on the outer wall of the upper part of the fermentation tank body (1); a first sealing ring (102) is provided in the first groove; a first annular groove corresponding to the annular protrusion (101) is provided in the sealing cover (4); inclined surfaces (4011) inclined upward are provided at the bottom of both sides of the cross-section of the first annular groove; an inverted V-shaped sealing ring (401) capable of closely fitting with the annular protrusion (101) is provided in the first annular groove; an outer boss (403) extends downward from the outer periphery of the sealing cover (4); the inner wall of the outer boss (403) closely fits with the first sealing ring (102).

2. A highly airtight polyglutamic acid fermentation device according to claim 1, characterized in that: The side of the first sealing ring (102) close to the outer boss (403) is an arc-shaped structure, and a stopper (103) is provided at the bottom of the first groove for preventing the first sealing ring (102) from falling out.

3. A highly airtight polyglutamic acid fermentation device according to claim 1, characterized in that: The locking mechanism (5) comprises a fixing seat (501), the fixing seat (501) being fixedly connected to the outer wall of the fermentation tank body (1), a pin shaft (503) being rotatably connected to the fixing seat (501), a threaded rod (502) being fixedly connected to the pin shaft (503), and a locking nut (504) being threadedly connected to the threaded rod (502); an annular clamping plate being fixedly connected to the outer side of the outer boss (403), and a plurality of clamping grooves (402) for fixing the locking mechanism (5) are provided on the annular clamping plate.

4. A highly airtight polyglutamic acid fermentation device according to claim 3, characterized in that: The outer side of the clamping groove (402) passes through an annular clamping plate, and the locking nut (504) abuts against the upper end surface of the annular clamping plate.

5. The highly airtight polyglutamic acid fermentation device according to claim 1, characterized in that: A reduction motor (7) is fixedly mounted on the sealing cover (4); an output shaft of the reduction motor (7) passes through the sealing cover (4) and is rotatably connected to the sealing cover (4) in a sealed manner; a stirring and lifting mechanism (9) is fixedly connected to the output shaft of the reduction motor (7); an air inlet pipe (8) passes through the sealing cover (4); the bottom of the air inlet pipe (8) extends to the lower part of the fermentation tank body (1); and a discharge port (3) is provided at the bottom of the fermentation tank body (1).

6. A highly airtight polyglutamic acid fermentation device according to claim 5, characterized in that: The stirring and lifting mechanism (9) comprises a stirring rod (901), the upper end of which is fixedly connected to the output shaft of the reduction motor (7) via a coupling, the stirring rod (901) being provided with a detachable spiral lifting blade (902), the other end of which is fixedly connected to a large rotating disk (903), and the outer circumference of which is fixedly connected to a plurality of evenly distributed stirring blades (904).

7. A highly airtight polyglutamic acid fermentation device according to claim 6, characterized in that: The diameter of the large turntable (903) is greater than the diameter of the spiral lifting blade (902).

8. A highly airtight polyglutamic acid fermentation device according to any one of claims 1 to 7, characterized in that: The sealing cover (4) is also provided with a feeding mechanism (6), the feeding mechanism (6) comprising a feeding pipe and a supply pipe, the feeding pipe is fixedly connected to the sealing cover (4), a lower flange (602) is sealingly fixedly connected to the feeding pipe, the lower flange (602) is connected to the upper flange (601) by bolts, a second annular groove is formed on the upper flange (601), a second sealing ring (603) is provided in the second annular groove, the upper and lower end surfaces of the outer circumference of the second sealing ring (603) are respectively provided with protrusions (604), and an annular clamping block corresponding to the second annular groove is provided on the lower flange (602), and the annular clamping block is tightly matched with the second sealing ring (603).

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