DNA extraction and detection device for single-channel pipettor

Abstract

The utility model relates to the technical field of pipettes, and discloses a DNA (deoxyribonucleic acid) extraction and detection device for a single-channel pipettor, which comprises a pipettor body, an extracting solution adding component, a pipetting device and a mixing device, the extracting solution adding component is arranged on the side surface of the pipettor body, the pipetting device is arranged on the side surface of the pipettor body, and the mixing device is arranged on the side surface of the pipettor body. The mixing device is arranged in the pipettor body, the mixing device is composed of an electric push rod, a moving disc, a circular ring, a motor, a first gear, a rotating shaft, a second gear and stirring blades, the electric push rod is fixedly installed in the pipettor body, and the moving disc is slidably installed in the pipettor body; and the circular ring is rotationally mounted at the bottom of the movable disc. According to the DNA extraction and detection device for the single-channel pipettor, the electric push rod is started to drive the moving disc to descend, and the rotating shaft can rotate by driving the motor, so that liquid in the DNA extraction chamber can be fully stirred by the stirring blades, and the DNA extraction and detection device is convenient to use.

Description

Technical Field

[0001] The utility model relates to the technical field of pipettes, in particular to a DNA extraction and detection device for a single-channel pipette. Background Art

[0002] As one of the core components of the automated pipetting workstation, the pipette is a commonly used device in biochemical laboratories. It is mainly used for operations such as liquid transfer and distribution. Among them, pipetting operations are often used for sample transfer, reagent addition, liquid mixing, etc. DNA extraction is a technology often required in laboratories. Generally, extraction reagents are added to the sample, and after the extraction reagents extract DNA, the sample is allowed to stand and separate. The DNA-containing reagent can then be separated.

[0003] The patent application number is 201822065334.3. It discloses a DNA extraction and detection device for a single-channel pipette, including a pipette housing, an extraction liquid adder is fixedly provided on one side of the pipette housing, a pipette main pipe is fixedly connected to the middle of the bottom end of the pipette housing, both sides of the bottom end of the pipette main pipe are connected to pipette branches, a first solenoid valve is fixedly provided on the two pipette branches, a control room is fixedly provided at the top of the pipette housing, a battery and a microcontroller are fixedly provided at the top of the control room, an electric cylinder is fixedly installed in the middle of the bottom end of the control room, and an exhaust pipe is fixedly provided in the middle of the pipette housing.

[0004] The single-channel pipette DNA extraction and detection device facilitates the extraction of DNA within the pipette housing, avoids multiple pipetting tasks, and reduces experimental errors. However, there are still areas that can be improved. For example, when the device discharges the extracted DNA, it passes through the pipette main tube, resulting in cross-mixing, which reduces the purity of the DNA extraction. In addition, the device needs to be manually shaken to mix the DNA extraction solution and the DNA sample solution, which is inconvenient to use and cannot meet current needs. Utility Model Content

[0005] The purpose of the utility model is to provide a DNA extraction and detection device for a single-channel pipette to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a DNA extraction and detection device for a single-channel pipette, comprising a pipette body, an extraction liquid adding component, a pipette device, and a mixing device, wherein the extraction liquid adding component is arranged on the side of the pipette body, the pipette device is arranged on the side of the pipette body, and the mixing device is arranged inside the pipette body.

[0007] The mixing device is composed of an electric push rod, a moving disk, a ring, a motor, a first gear, a rotating shaft, a second gear, and a stirring blade. The electric push rod is fixedly installed inside the pipette body, the moving disk is slidably installed inside the pipette body, the ring is rotatably installed at the bottom of the moving disk, the motor is fixedly installed at the top of the moving disk, the first gear is fixedly installed on the output shaft of the motor, the rotating shaft is rotatably installed at the bottom of the moving disk, the second gear is fixedly installed on the surface of the rotating shaft, and the stirring blade is fixedly installed on the surface of the rotating shaft.

[0008] Preferably, the pipetting device is composed of a liquid inlet tube, a first solenoid valve, a liquid outlet tube, and a second solenoid valve. The liquid inlet tube is fixedly installed on the side of the pipette body, the first solenoid valve is fixedly installed on the surface of the liquid inlet tube, the liquid outlet tube is fixedly installed on the bottom of the pipette body, and the second solenoid valve is fixedly installed on the surface of the liquid outlet tube. The provision of the pipetting device can avoid cross-mixing with other liquids throughout the process of discharging the extracted DNA, thereby effectively improving the purity of the extracted DNA.

[0009] Preferably, a DNA extraction chamber is provided inside the pipette body, and the DNA extraction chamber is connected to a liquid inlet pipe and a liquid outlet pipe. This arrangement can introduce liquid into the DNA extraction chamber through the liquid inlet pipe, and discharge the liquid in the DNA extraction chamber through the liquid outlet pipe.

[0010] Preferably, a battery is provided inside the pipette body, and the battery is electrically connected to the electric push rod, the motor, the first solenoid valve, and the second solenoid valve. This arrangement can convert chemical energy into electrical energy through the battery to power the electric push rod, the motor, the first solenoid valve, and the second solenoid valve.

[0011] Preferably, a first tooth is provided on the outer side of the circular ring, and the first tooth is meshed with the first gear. The driving motor can make the circular ring rotate under the action of the first tooth and the first gear.

[0012] Preferably, a second tooth is provided on the inner side of the circular ring, and the second tooth is meshed with the second gear. By rotating the circular ring, the rotating shaft can be rotated under the action of the second tooth and the second gear.

[0013] Preferably, the rotating shaft, the second gear and the stirring blade are all grouped together and distributed in a circular array at the bottom of the movable plate. Providing multiple groups of such components can more fully stir the liquid in the DNA extraction chamber.

[0014] Preferably, the movable plate is fixedly mounted on the bottom of the electric push rod, and starting the electric push rod can drive the movable plate to move up and down.

[0015] Preferably, a through hole matching the stirring blade is provided inside the pipette body, and this arrangement enables the stirring blade to pass through the through hole to stir the liquid in the DNA extraction chamber.

[0016] Preferably, an observation window is provided on the side of the pipette body, which facilitates observation of the liquid stratification in the DNA extraction chamber.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] (1) The single-channel pipette uses a DNA extraction detection device to start the electric push rod to drive the moving plate to descend, and the driving motor can rotate the rotating shaft, so that the stirring blade can fully stir the liquid in the DNA extraction chamber, which is convenient to use.

[0019] (2) The single-channel pipette DNA extraction and detection device can avoid cross-mixing with other liquids during the discharge of the extracted DNA by separately arranging the liquid inlet tube and the liquid outlet tube, thereby effectively improving the purity of the DNA extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0022] Figure 3 This is a schematic diagram of the electric push rod, the moving plate and the rotating shaft structure of the utility model;

[0023] Figure 4 This is a schematic diagram of the structure of the circular ring, the motor and the first gear of the utility model.

[0024] In the figure: 1. pipette body; 2. extracting liquid adding component; 3. pipetting device; 301. liquid inlet pipe; 302. first solenoid valve; 303. liquid outlet pipe; 304. second solenoid valve; 4. mixing device; 401. electric push rod; 402. moving disk; 403. ring; 404. motor; 405. first gear; 406. rotating shaft; 407. second gear; 408. stirring blade. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] See also Figure 1-4 The utility model provides a technical solution: a DNA extraction and detection device for a single-channel pipette, comprising a pipette body 1, an extraction liquid adding component 2, a pipette device 3, and a mixing device 4. The extraction liquid adding component 2 is arranged on the side of the pipette body 1, and an observation window is arranged on the side of the pipette body 1. The arrangement is convenient for observing the liquid stratification in the DNA extraction chamber, the pipette device 3 is arranged on the side of the pipette body 1, and the mixing device 4 is arranged inside the pipette body 1.

[0027] The pipette device 3 is composed of a liquid inlet pipe 301, a first electromagnetic valve 302, a liquid outlet pipe 303, and a second electromagnetic valve 304. The liquid inlet pipe 301 is fixedly installed on the side of the pipette body 1, the first electromagnetic valve 302 is fixedly installed on the surface of the liquid inlet pipe 301, and the liquid outlet pipe 303 is fixedly installed on the bottom of the pipette body 1. A DNA extraction chamber is provided inside the pipette body 1. The DNA extraction chamber is connected to the liquid inlet pipe 301 and the liquid outlet pipe 303. This arrangement can introduce liquid into the DNA extraction chamber through the liquid inlet pipe 301, and remove the liquid in the DNA extraction chamber through the liquid outlet pipe 303. The liquid is discharged, and the second solenoid valve 304 is fixedly installed on the surface of the liquid outlet tube 303. A battery is arranged inside the pipette body 1, and the battery and the electric push rod 401, the motor 404, the first solenoid valve 302, and the second solenoid valve 304 are electrically connected. This arrangement can convert chemical energy into electrical energy through the battery to power the electric push rod 401, the motor 404, the first solenoid valve 302, and the second solenoid valve 304. A pipetting device 3 is arranged, which can avoid cross-mixing with other liquids during the discharge of the extracted DNA, thereby effectively improving the purity of the DNA extraction.

[0028] The mixing device 4 is composed of an electric push rod 401, a moving disk 402, a ring 403, a motor 404, a first gear 405, a rotating shaft 406, a second gear 407, and a stirring blade 408. The electric push rod 401 is fixedly installed inside the pipette body 1, and the moving disk 402 is slidably installed inside the pipette body 1. The moving disk 402 is fixedly installed at the bottom of the electric push rod 401. Starting the electric push rod 401 can drive the moving disk 402 to rise and fall. The ring 403 is rotatably installed at the bottom of the moving disk 402, and the motor 404 is fixedly installed on the top of the moving disk 402. The first gear 405 is fixedly installed on the output shaft of the motor 404. The outer side of the ring 403 is provided with a first tooth, and the first tooth is meshed with the first gear 405. The driving motor 404 can make the first tooth and the first gear 405 work. The ring 403 rotates, and the rotating shaft 406 is rotatably installed at the bottom of the movable disk 402. The second gear 407 is fixedly installed on the surface of the rotating shaft 406. The inner side of the ring 403 is provided with a second tooth, and the second tooth and the second gear 407 are meshed. By rotating the ring 403, the rotating shaft 406 can be rotated under the action of the second tooth and the second gear 407. The stirring blade 408 is fixedly installed on the surface of the rotating shaft 406. A through hole matching the stirring blade 408 is opened inside the pipette body 1. This setting allows the stirring blade 408 to pass through the through hole to stir the liquid in the DNA extraction chamber. The rotating shaft 406, the second gear 407 and the stirring blade 408 are all grouped together and distributed in a circular array at the bottom of the movable disk 402. A plurality of groups of this component are provided to stir the liquid in the DNA extraction chamber more fully.

[0029] When in use, open the first solenoid valve 302, introduce the liquid into the DNA extraction chamber through the liquid inlet pipe 301, add the extract to the DNA extraction chamber through the extract adding component 2, start the electric push rod 401 to drive the movable plate 402 to descend, drive the motor 404, and under the action of the first teeth and the first gear 405, the ring 403 can be rotated, and under the action of the second teeth and the second gear 407, the rotating shaft 406 can be rotated, so that the stirring blade 408 can fully stir the liquid in the DNA extraction chamber. After the stirring is completed, start the electric push rod 401 to drive the movable plate 402 to rise, let the liquid stand for a period of time, observe the liquid stratification in the DNA extraction chamber through the observation window, and then open the second solenoid valve 304 to discharge the liquid in the DNA extraction chamber through the liquid outlet pipe 303.

[0030] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0031] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention.

Claims

1. A DNA extraction and detection device for a single-channel pipette, comprising a pipette body (1), an extracting liquid adding component (2), a pipetting device (3), and a mixing device (4), characterized in that: The extract adding component (2) is arranged on the side of the pipette body (1), the pipetting device (3) is arranged on the side of the pipette body (1), and the mixing device (4) is arranged inside the pipette body (1); The mixing device (4) is composed of an electric push rod (401), a moving disk (402), a ring (403), a motor (404), a first gear (405), a rotating shaft (406), a second gear (407), and a stirring blade (408). The electric push rod (401) is fixedly installed inside the pipette body (1), the moving disk (402) is slidably installed inside the pipette body (1), the ring (403) is rotatably installed on the bottom of the moving disk (402), the motor (404) is fixedly installed on the top of the moving disk (402), the first gear (405) is fixedly installed on the output shaft of the motor (404), the rotating shaft (406) is rotatably installed on the bottom of the moving disk (402), the second gear (407) is fixedly installed on the surface of the rotating shaft (406), and the stirring blade (408) is fixedly installed on the surface of the rotating shaft (406).

2. A DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: The liquid transfer device (3) is composed of a liquid inlet tube (301), a first electromagnetic valve (302), a liquid outlet tube (303), and a second electromagnetic valve (304); the liquid inlet tube (301) is fixedly installed on the side of the liquid transfer device body (1); the first electromagnetic valve (302) is fixedly installed on the surface of the liquid inlet tube (301); the liquid outlet tube (303) is fixedly installed on the bottom of the liquid transfer device body (1); and the second electromagnetic valve (304) is fixedly installed on the surface of the liquid outlet tube (303).

3. A DNA extraction and detection device for a single-channel pipette according to claim 2, characterized in that: A DNA extraction chamber is provided inside the pipette body (1), and the DNA extraction chamber is connected to a liquid inlet pipe (301) and a liquid outlet pipe (303).

4. The DNA extraction and detection device for a single-channel pipette according to claim 2, characterized in that: A storage battery is arranged inside the pipette body (1), and the storage battery is electrically connected to the electric push rod (401), the motor (404), the first solenoid valve (302), and the second solenoid valve (304).

5. The single-channel pipette DNA extraction and detection device according to claim 1, characterized in that: The outer side of the circular ring (403) is provided with first teeth, and the first teeth are meshed with the first gear (405).

6. The DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: The inner side of the circular ring (403) is provided with second teeth, and the second teeth are meshed with the second gear (407).

7. The DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: The rotating shaft (406), the second gear (407) and the stirring blade (408) are arranged in a group and are distributed in a circular array at the bottom of the moving plate (402).

8. The DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: The moving plate (402) is fixedly mounted on the bottom of the electric push rod (401).

9. The DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: The interior of the pipette body (1) is provided with a through hole matching the stirring blade (408).

10. The DNA extraction and detection device for a single-channel pipette according to claim 1, characterized in that: An observation window is provided on the side of the pipette body (1).

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