Novel culture bottle suitable for tissue culture

Abstract

The utility model relates to the technical field of culture bottles, and discloses a novel culture bottle suitable for tissue culture, which comprises a culture bottle body, the top of the culture bottle body is fixedly connected with a bottle opening, the interior of the culture bottle body is fixedly connected with a first baffle plate and a second baffle plate, one side of the first baffle plate is an inoculation area, and the other side of the second baffle plate is a liquid changing area. The inner wall of the second baffle is fixedly connected with a filter screen. According to the novel culture bottle suitable for tissue culture, when cells are cultured by tissue blocks, when the tissue blocks are 1-3mm in diameter, the cells climb out more quickly, the quantity of the cells is larger, the tissue blocks with proper sizes can be screened by the novel culture bottle body to enter the culture bottle body for culture, and the cell growth efficiency is improved; when the novel culture bottle body is used for liquid change, passage or cryopreservation, a tissue block can be separated from a culture solution, and a transfer pipette cannot be blocked, so that the operation becomes smoother, the operation efficiency is improved, and the probability of pollution is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of culture bottles, in particular to a new culture bottle suitable for tissue culture. Background Art

[0002] The in vitro culture of cells and tissues has become an indispensable part of life research and practice. There are many types of cells cultured, ranging from viruses to bacteria and fungi, from human cells to animal cells and plant cells.

[0003] A Chinese patent discloses a new type of tissue culture bottle (authorization announcement number CN211861397U). This patented technology can fill the bottle with flowing sterile air through the combined work of a blower fan and a bacterial filter, which is suitable for culturing aerobic plant tissues.

[0004] With respect to the above-mentioned and existing related technologies, the inventors believe that the following defects often exist: tissue blocks for culturing cells need to be cut into pieces with a diameter of one to three millimeters, but there may be too large tissue blocks in the process, which will affect the cell crawling out and proliferation. To ensure the cell crawling out and proliferation, a small amount of culture medium should be added to make the tissue blocks adhere to the wall, but insufficient nutrition requires multiple small changes of the medium. When changing the medium, it is necessary to prevent the tissue blocks from floating and remove the old culture medium, but the operation is easy to clog the pipette, increasing the difficulty and risk of the operation. At the same time, during passaging and freezing, it may not be possible to completely separate the tissue blocks and the culture medium, affecting subsequent experiments. Cell culture needs to streamline the steps, and unsmooth operation may lead to contamination. Utility Model Content

[0005] The technical problem to be solved by the utility model is that in the prior art, the tissue block culture cells need to be cut into 1-3 mm diameters to avoid the cell crawling out and proliferation being affected by the large diameters. A small amount of culture fluid is added to make the tissue block adhere to the wall, but the fluid needs to be changed in small amounts and multiple times to avoid nutrient deficiency. When changing the fluid, the tissue block needs to be prevented from floating and the old fluid needs to be removed. However, the operation is easy to block the pipette, increasing the difficulty and risk. During passage and freezing, the tissue block and the culture fluid may be difficult to separate, affecting the experiment. Cell culture needs to streamline the steps to avoid the disadvantage of contamination caused by unsmooth operation. For this reason, we propose a new culture bottle suitable for tissue culture.

[0006] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme: a new type of culture bottle suitable for tissue culture, comprising a culture bottle body, the top of the culture bottle body is fixedly connected to a bottle mouth, the surface of the bottle mouth is threadedly connected to a breathable bottle cap, the interior of the culture bottle body is fixedly connected to a first baffle and a second baffle, the number of the first baffle and the second baffle is multiple, one side of the first baffle is an inoculation area, and the other side of the second baffle is a liquid exchange area.

[0007] Preferably, the spacing between the first baffles is three millimeters.

[0008] Preferably, a filter screen is fixedly connected to the inner wall of the second baffle.

[0009] Preferably, the filter screen is made of nylon.

[0010] Preferably, the filter has a preset pore size, which allows the culture medium and cells to pass smoothly.

[0011] Preferably, a microporous filter membrane is fixedly connected to the top of the inner cavity of the breathable bottle cap.

[0012] Technical effects and advantages of the utility model:

[0013] In the utility model, when cells are cultured with tissue blocks, when the size of the tissue blocks is between one millimeter and three millimeters in diameter, the cells crawl out faster and the cell quantity is greater. The novel culture bottle body can screen tissue blocks of appropriate size to enter the middle of the culture bottle body for culture, thereby improving the efficiency of cell growth. When performing operations such as fluid replacement, passage or freezing, the novel culture bottle body can separate the tissue blocks from the culture fluid without clogging the pipette, thereby making the operation smoother, improving the efficiency of the operation and reducing the probability of contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0016] Figure 3 This is a schematic diagram of the filter screen split structure of the utility model;

[0017] Figure 4 It is a schematic diagram of the disassembly structure of the breathable bottle cap of the utility model.

[0018] Legend: 1. Culture bottle body; 2. Bottle mouth; 3. Breathable bottle cap; 4. First baffle; 5. Second baffle; 6. Filter screen; 7. Microporous filter membrane; 8. Inoculation area; 9. Liquid exchange area. DETAILED DESCRIPTION

[0019] The present invention will now be further described in detail in conjunction with the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0020] Reference Figure 1 , Figure 2 and Figure 3As shown, the utility model provides a technical solution: a new culture bottle suitable for tissue culture, comprising a culture bottle body 1, a bottle mouth 2 is fixedly connected to the top of the culture bottle body 1, a breathable bottle cap 3 is threadedly connected to the surface of the bottle mouth 2, a first baffle 4 and a second baffle 5 are fixedly connected to the inside of the culture bottle body 1, the number of the first baffle 4 and the second baffle 5 is multiple, one side of the first baffle 4 is an inoculation area 8, the other side of the second baffle 5 is a liquid replacement area 9, the inner wall of the second baffle 5 is fixedly connected to a filter screen 6, when inoculation, after the tissue block is cut into pieces, an appropriate amount of culture solution is poured in and shaken, the culture breathable bottle cap 3 is opened, the culture bottle body 1 is tilted vertically to the left, the tissue block is poured into the inoculation area 8 along the left side wall of the culture bottle body 1, and then the breathable bottle cap 3 is covered, the culture bottle body 1 is laid flat, and the culture bottle body 1 is gently shaken so that the tissue block of appropriate size enters The culture bottle body 1 is placed in the middle of the culture bottle, and then the culture bottle body 1 is placed in the incubator for culture; when performing the liquid replacement operation, the breathable bottle cover 3 is opened, the culture bottle body 1 is slowly tilted to the right, so that the culture solution passes through the filter 6 and enters the liquid replacement area 9, the pipette enters the liquid replacement area 9 from the bottle mouth 2, the old culture solution is sucked away, and then new culture solution is added from the liquid replacement area 9, the breathable bottle cover 3 is covered, and the culture bottle body 1 is placed flat in the incubator; when subculturing or freezing, digestion is performed, the breathable bottle cover 3 is opened, the pipette enters the liquid replacement area 9 from the bottle mouth 2 to remove the old culture medium, and then sterile PBS buffer is added from the liquid replacement area 9 for washing, after the buffer is removed, digestive enzymes are added from the liquid replacement area 9 for digestion, and after the cells fall off, reagents are added from the liquid replacement area 9 to terminate digestion, the culture bottle body 1 is tilted to the right, the culture solution containing cells is separated from the tissue block, and the culture solution is sucked from the liquid replacement area 9 into a centrifuge tube and waited for centrifugation.

[0021] Reference Figure 2 As shown, in this embodiment: the distance between the first baffles 4 is three millimeters, and the inoculation area 8 is separated by several first baffles 4 on the left side of the culture bottle body 1. The distance between the first baffles 4 is three millimeters, which is used to screen tissue blocks of appropriate size to enter the middle of the culture bottle body 1 for culture when inoculating tissue blocks, so that the tissue blocks can be more evenly distributed during the culture process, thereby improving the culture efficiency.

[0022] Reference Figure 3 As shown, in this embodiment: the material of the filter 6 is nylon. The nylon filter 6, with its excellent durability and efficient filtering performance, further enhances the overall advantage of the product. Compared with traditional materials, the nylon filter 6 not only has stronger wear resistance, can maintain a stable shape during long-term use, and reduce the performance degradation caused by physical wear, but also shows excellent chemical corrosion resistance, ensuring that it can still work stably in a variety of complex environments, thereby extending the service life of the product.

[0023] Reference Figure 3As shown, in this embodiment: the filter 6 has a preset pore size, which allows the culture fluid and cells to pass smoothly. The pore size of the filter 6 has been carefully calculated and experimentally verified to ensure that while allowing the culture fluid and cells to flow unimpeded, it effectively blocks microorganisms, particulate impurities and larger cell fragments that may contaminate the culture environment. This fine filtering effect creates a purer and undisturbed microenvironment for cell growth, helps to improve the purity and health of cell culture, and reduces the risk of experimental failure due to contamination.

[0024] Reference Figure 4 As shown, in this embodiment: a microporous filter membrane 7 is fixedly connected to the top of the inner cavity of the breathable bottle cap 3, and the microporous filter membrane 7 is a refined hydrophobic filter membrane. The chemically stable membrane complies with the USP Class VI mark and does not contain animal-derived substances, ensuring a close fit with the bottle mouth 2, providing an effective barrier to microorganisms, and ensuring a constant ventilation airflow, thereby maintaining the stability and sterility of the internal environment of the culture bottle.

[0025] Working principle: When inoculating, cut the tissue block into pieces, pour in an appropriate amount of culture solution and shake well, open the culture breathable bottle cap 3, tilt the culture bottle body 1 vertically to the left, pour the tissue block along the left side wall of the culture bottle body 1 into the inoculation area 8, then cover the breathable bottle cap 3, lay the culture bottle body 1 flat, gently shake the culture bottle body 1 so that the tissue block of appropriate size enters the middle of the culture bottle, and then put the culture bottle body 1 into the incubator for culture; when changing the liquid, open the breathable bottle cap 3, slowly tilt the culture bottle body 1 to the right, so that the culture solution passes through the filter 6 into the liquid change area 9, and pipette from the bottle The bottle body 1 is placed flat in the incubator; when subculturing or freezing, digestion is performed, the breathable bottle cap 3 is opened, the pipette enters the liquid exchange area 9 from the bottle mouth 2 to remove the old culture medium, and then sterile PBS buffer is added from the liquid exchange area 9 for washing. After the buffer is removed, digestive enzymes are added from the liquid exchange area 9 for digestion. After the cells fall off, reagents are added from the liquid exchange area 9 to terminate the digestion. The body 1 of the culture bottle is tilted to the right to separate the culture medium containing the cells from the tissue blocks, and the culture medium is sucked from the liquid exchange area 9 into a centrifuge tube and waited for centrifugation. The inoculation area 8 is separated by several first baffles 4 on the left side of the culture bottle body 1. The distance between the first baffles 4 is three millimeters. When the tissue blocks are inoculated, the tissue blocks are screened to enter the middle of the culture bottle body 1 for cultivation, so that the tissue blocks can be more evenly distributed during the cultivation process, thereby improving the cultivation efficiency. The nylon filter 6, with its excellent durability and efficient filtering performance, further enhances the overall advantages of the product. Compared with traditional materials, the nylon filter 6 not only has stronger wear resistance, but also can maintain a stable shape during long-term use, reducing the damage caused by physical wear. It can not only reduce the risk of cell death, but also exhibits excellent chemical corrosion resistance, ensuring that it can still work stably in a variety of complex environments and extending the service life of the product. The pore size of the filter 6 has been carefully calculated and experimentally verified to ensure that while allowing the culture solution and cells to flow unimpeded, it effectively blocks particulate impurities and larger cell fragments. This fine filtering effect creates a more stable and uniform growth environment for cell growth, helps to improve the purity of cell culture, and reduces the risk of experimental failure caused by contamination introduced by multi-step operations. The microporous filter membrane 7 is a refined hydrophobic and chemically stable membrane that meets the USP VI grade mark and does not contain animal-derived substances, ensuring a close fit with the bottle mouth 2, providing an effective barrier to microorganisms, and ensuring constant ventilation airflow, thereby maintaining the stability and sterility of the internal environment of the culture bottle.

[0026] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A novel culture bottle suitable for tissue culture, comprising a culture bottle body (1), characterized in that: The top of the culture bottle body (1) is fixedly connected with a bottle mouth (2), and the surface of the bottle mouth (2) is threadedly connected with a breathable bottle cap (3). The interior of the culture bottle body (1) is fixedly connected with a first baffle (4) and a second baffle (5), and the number of the first baffle (4) and the second baffle (5) is multiple. One side of the first baffle (4) is an inoculation area (8), and the other side of the second baffle (5) is a liquid replacement area (9).

2. A novel culture bottle suitable for tissue culture according to claim 1, characterized in that: The spacing between the first baffles (4) is three millimeters.

3. A novel culture bottle suitable for tissue culture according to claim 1, characterized in that: A filter screen (6) is fixedly connected to the inner wall of the second baffle (5).

4. A novel culture bottle suitable for tissue culture according to claim 3, characterized in that: The filter screen (6) is made of nylon.

5. A novel culture bottle suitable for tissue culture according to claim 3, characterized in that: The filter (6) has a preset pore size, which allows the culture fluid and cells to pass smoothly.

6. A novel culture bottle suitable for tissue culture according to claim 1, characterized in that: A microporous filter membrane (7) is fixedly connected to the top of the inner cavity of the air-permeable bottle cap (3).

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