Biological membrane culture and storage device based on anaerobic ammonia oxidation

By designing a culture storage device based on anaerobic ammonia oxidized biofilm, using disassembly and assembly structure and limit structure, the problem of biofilm damage caused by movement of the culture box in the existing device is solved, and the stability and efficient cultivation of the biofilm are achieved.

CN222961397UActive Publication Date: 2025-06-10袁云霞
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Patent Information

Application Number
CN202421549516.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-03
Publication Date
2025-06-10
Estimated Expiration
2034-07-03

AI Technical Summary

Technical Problem

In the existing anaerobic ammonia oxidized biofilm culture device, the culture box is prone to move and lead to collisions, destroying the integrity of the biofilm.

Method used

A culture storage device based on anaerobic ammonia oxidized biofilm is designed, adopting a disassembly structure and a limiting structure, the built-in box is positioned through the adsorption force of the suction cup and the fixing plate, and the sealing cap and the culture storage box are ensured by the engagement connection between the insertion rod and the insertion hole, and the sealing cap and the culture storage box are maintained at an oxygen-free state through the air pump, and the temperature controller is adjusted.

Benefits of technology

Effectively prevent the built-in box from shaking and collision, maintain the integrity of the biofilm, and at the same time facilitate assembly and cleaning of porous ceramics, ensuring that microorganisms can adhere well to form biofilms.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a biological membrane culture and storage device based on anaerobic ammonia oxidation, including culture and storage box and sucker, the culture and storage box is internally provided with built-in box, the built-in box is internally provided with fixing rod, the upper part of the fixing rod is provided with porous ceramic, and the middle part of the surface of the porous ceramic is provided with a mounting hole. The suction cup is arranged on the outer wall of the built-in box, and a fixing plate is arranged at the end of the suction cup. According to the culture and storage device based on the anaerobic ammonia oxidation biological membrane, the built-in box can be positioned through adsorption force between a suction cup and a fixing plate, the built-in box is effectively prevented from shaking in the culture and storage box, collision of the built-in box is avoided, a temperature controller can adjust the temperature of a heating pipe, and the temperature of the heating pipe can be adjusted. The temperature in the culture storage box is convenient to adjust, a certain temperature environment is provided for anaerobic ammonia oxidation biological membrane culture, and the air pump directly pumps air in the culture storage box, so that the culture storage box is kept in an anaerobic state.
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Description

Technical Field

[0001] The utility model relates to the technical field of anaerobic ammonium oxidation biofilms, in particular to a culture and storage device based on anaerobic ammonium oxidation biofilms. Background Art

[0002] Anaerobic ammonium oxidation biofilm is a biofilm composed of a group of microorganisms that can carry out ammonia oxidation reactions in an environment without oxygen. This biofilm plays an important role in the treatment of wastewater and sewage. The microorganisms in the anaerobic ammonium oxidation biofilm use the symbiotic relationship between ammonia oxidizing bacteria and denitrifying bacteria to complete the removal of ammonia nitrogen from wastewater through their respective metabolic activities. Ammonia oxidizing bacteria convert ammonia nitrogen into nitrite, while denitrifying bacteria reduce nitrite to nitrogen gas. This process does not require oxygen and can therefore be carried out under anaerobic conditions. The formation of anaerobic ammonium oxidation biofilm requires a suitable biological carrier, usually a porous material such as a sponge-like polymer or porous ceramic. On this carrier, microorganisms can attach and form a biofilm. This biofilm has high activity and biological quality, which can improve the efficiency of ammonia oxidation reactions. Anaerobic ammonium oxidation biofilm technology has been widely used in the field of wastewater treatment. Compared with traditional aerated biological treatment methods, anaerobic ammonium oxidation biofilm technology has a higher ammonia oxidation rate and lower energy consumption. In addition, anaerobic ammonium oxidation biofilm technology can also remove ammonia nitrogen and organic matter at the same time, making wastewater treatment more efficient. When cultivating anaerobic ammonium oxidation biofilm, a storage device is required for cultivation, and the storage device is in an anaerobic state.

[0003] As shown in the application number: CN201610443248.4, the present invention relates to a rapid enrichment and cultivation device for anaerobic ammonia-oxidizing bacteria, and belongs to the technical field of enrichment and cultivation of anaerobic ammonia-oxidizing bacteria and treatment of high-concentration ammonia nitrogen wastewater in environmental engineering. The anaerobic membrane bioreactor and the sampling system of the present invention are the main parts. The bottom end of the outlet pipe (12) of the anaerobic membrane bioreactor is consolidated with the membrane assembly (7) through a three-way valve (8), and a filler (18) is provided inside the inner cylinder body (6). The sampling system is used to take out the biofilm sample and the suspension sample on the filler to reduce the influence of sampling on the operation of the reactor. By adding fillers to the anaerobic membrane bioreactor to form a biofilm, and utilizing the mechanical friction effect of the filler on the membrane filament, the membrane pollution is effectively slowed down while ensuring the efficient enrichment of anaerobic ammonia-oxidizing bacteria. The present invention has the characteristics of high volumetric load, good effluent quality, high degree of automation, and stable operation, which can effectively slow down the pollution process and is suitable for the enrichment and cultivation of anaerobic ammonia-oxidizing bacteria and the treatment of high-concentration ammonia nitrogen wastewater.

[0004] Similar to the above application, there are still the following deficiencies:

[0005] The culture boxes inside the existing device will move, causing collisions between the culture boxes, causing the anaerobic ammonium oxidizing biofilm to move and destroy the integrity of the anaerobic ammonium oxidizing biofilm.

[0006] Therefore, in view of this, the existing structure and deficiencies are studied and improved, and a biofilm culture and storage device based on anaerobic ammonia oxidation is proposed, in order to achieve a more practical purpose. Utility Model Content

[0007] The purpose of the utility model is to provide a biofilm culture storage device based on anaerobic ammonia oxidation to solve the problems raised in the above background technology.

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a biofilm culture and storage device based on anaerobic ammonia oxidation, comprising a culture and storage box and a suction cup, wherein a built-in box is installed inside the culture and storage box, and a fixing rod is arranged inside the built-in box, a porous ceramic is arranged above the fixing rod, and a mounting hole is opened in the middle of the surface of the porous ceramic, the suction cup is arranged on the outer wall of the built-in box, and a fixing plate is arranged at the end of the suction cup.

[0009] Furthermore, a connecting strip is provided on the outer wall of the culture storage box, and an insertion hole is provided inside the connecting strip.

[0010] Furthermore, a sealing cover is arranged above the culture storage box, and an insertion rod is fixed to the outer wall of the sealing cover.

[0011] Furthermore, the number of the insertion rods is consistent with the number of the insertion holes, and the insertion rods are snap-fitted to the insertion holes.

[0012] Furthermore, temperature controllers are arranged on the left and right sides of the upper end of the sealing cover, and an air pump is arranged in the middle of the upper end of the sealing cover, and an air outlet is arranged at the upper end of the air pump.

[0013] Furthermore, heating pipes are installed at the left and right ends of the inner wall of the sealing cover, and an air extraction port is installed in the middle of the inner wall of the sealing cover.

[0014] Compared with the prior art, the beneficial effects of the utility model are: this anaerobic ammonia oxidation biofilm culture and storage device adopts a disassembly and assembly structure, which is convenient for assembling porous ceramics and also convenient for cleaning porous ceramics, and utilizes a limiting structure to ensure the stability of porous ceramics and effectively prevent porous ceramics from shaking.

[0015] 1. The adsorption force between the suction cup and the fixing plate of the present utility model can position the built-in box, effectively preventing the built-in box from shaking inside the culture storage box, avoiding collisions of the built-in box. The snap connection between the insertion rod and the insertion hole facilitates the assembly of the sealing cover and the culture storage box, ensuring the sealing performance between the two. The temperature controller can adjust the temperature of the heating tube, facilitating the adjustment of the temperature inside the culture storage box, providing a certain temperature environment for the cultivation of anaerobic ammonium oxidation biofilm. The air pump directly extracts the air inside the culture storage box, making the culture storage box maintain an anaerobic state. The mounting hole of the porous ceramic and the fixing rod form a socket connection, facilitating the positioning of the porous ceramic, and at the same time facilitating the disassembly and assembly of the porous ceramic, and the porous ceramic is convenient for microorganisms to adhere and form a biofilm. Description of the Drawings

[0016] Figure 1 It is a front view structural schematic diagram of a culture storage device based on anaerobic ammonium oxidation biofilm of the present utility model;

[0017] Figure 2 It is a bottom view three-dimensional structural schematic diagram of a culture storage device based on anaerobic ammonium oxidation biofilm of the present utility model;

[0018] Figure 3 It is a three-dimensional structural schematic diagram of the built-in box of a culture storage device based on anaerobic ammonium oxidation biofilm of the present utility model.

[0019] In the figure: 1, culture storage box; 2, connecting strip; 3, insertion hole; 4, built-in box; 5, porous ceramic; 6, mounting hole; 7, fixing plate; 8, suction cup; 9, sealing cover; 10, insertion rod; 11, temperature controller; 12, air pump; 13, air outlet; 14, heating tube; 15, air extraction port; 16, fixing rod. Detailed Embodiment

[0020] The following further describes in detail the embodiments of the present utility model in conjunction with the drawings and examples. The following examples are used to illustrate the present utility model, but cannot be used to limit the scope of the present utility model.

[0021] As Figures 1 - 3As shown in the figure, a cultivation and storage device for anaerobic ammonium oxidation biofilm includes a cultivation and storage box 1 and a suction cup 8. An inner box 4 is installed inside the cultivation and storage box 1, and a fixing rod 16 is arranged inside the inner box 4. Above the fixing rod 16, a porous ceramic 5 is placed. A mounting hole 6 is formed in the middle of the surface of the porous ceramic 5. The suction cup 8 is arranged on the outer wall of the inner box 4, and a fixing plate 7 is arranged at the end of the suction cup 8. A connecting strip 2 is arranged on the outer wall of the cultivation and storage box 1, and an insertion hole 3 is formed inside the connecting strip 2. A sealing cover 9 is arranged above the cultivation and storage box 1, and an insertion rod 10 is fixed on the outer wall of the sealing cover 9. The number of the insertion rods 10 is the same as that of the insertion holes 3, and the insertion rod 10 is in snap connection with the insertion hole 3. Temperature controllers 11 are arranged on the left and right sides of the upper end of the sealing cover 9, and an air extraction pump 12 is arranged in the middle of the upper end of the sealing cover 9. An air outlet 13 is arranged at the upper end of the air extraction pump 12. Heating pipes 14 are installed at the left and right ends of the inner wall of the sealing cover 9, and an air extraction port 15 is installed in the middle of the inner wall of the sealing cover 9. The adsorption force between the suction cup 8 and the fixing plate 7 can position the inner box 4, effectively prevent the inner box 4 from shaking inside the cultivation and storage box 1, and avoid collisions of the inner box 4. The snap connection between the insertion rod 10 and the insertion hole 3 facilitates the assembly of the sealing cover 9 and the cultivation and storage box 1 and ensures the sealing performance between the two. The temperature controller 11 can adjust the temperature of the heating pipe 14, so as to adjust the temperature inside the cultivation and storage box 1 and provide a certain temperature environment for the cultivation of anaerobic ammonium oxidation biofilm. The air extraction pump 12 directly extracts the air inside the cultivation and storage box 1 to make the cultivation and storage box 1 in an anaerobic state. The mounting hole 6 of the porous ceramic 5 and the fixing rod 16 form a socket connection, which is convenient for positioning the porous ceramic 5 and also facilitates the disassembly and assembly of the porous ceramic 5. Moreover, the porous ceramic 5 is convenient for microorganisms to attach and form a biofilm.

[0022] Working principle: When the cultivation and storage device for anaerobic ammonium oxidation biofilm is in use, first, according to Figures 1 - 3 , the mounting hole 6 in the middle of the porous ceramic 5 is sleeved with the fixing rod 16, and then the porous ceramic 5 can be fixed inside the inner box 4. After all the porous ceramics 5 are installed, the inner box 4 is placed into the cultivation and storage box 1. Press the suction cup 8 on the outer wall of the inner box 4 to make it adsorb to the fixing plate 7, and then the inner box 4 can be fixed. After being fixed, anaerobic ammonium oxidation bacteria are put into the inside of the porous ceramic 5. After the placement is completed, the sealing cover 9 is covered, and its insertion rod 10 is inserted into the insertion hole 3, and then the sealing cover 9 can be fixed. After being fixed, the air extraction pump 12 is started to extract the air inside the cultivation and storage box 1 to make the cultivation and storage box 1 in an anaerobic state. Then the temperature controller 11 is started to make the heating pipe 14 start to work, changing the temperature inside the cultivation and storage box 1 to make the temperature reach the growth temperature of anaerobic ammonium oxidation bacteria. At this time, anaerobic ammonium oxidation bacteria and microorganisms will grow and attach inside the porous ceramic 5 and gradually form an anaerobic ammonium oxidation biofilm.

[0023] The embodiments of the present utility model are given for purposes of illustration and description, and are not exhaustive or limit the present utility model to the disclosed forms. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described in order to better explain the principles and practical applications of the present utility model, and enable those of ordinary skill in the art to understand the present utility model and design various embodiments with various modifications suitable for specific purposes.

Claims

1. A biofilm culture and storage device based on anaerobic ammonium oxidation, comprising a culture and storage box (1) and a suction cup (8), characterized in that: The culture storage box (1) has a built-in box (4) installed inside, and a fixing rod (16) is arranged inside the built-in box (4), a porous ceramic (5) is arranged above the fixing rod (16), and a mounting hole (6) is opened in the middle of the surface of the porous ceramic (5), the suction cup (8) is arranged on the outer wall of the built-in box (4), and a fixing plate (7) is arranged at the end of the suction cup (8).

2. The anaerobic ammonium oxidation biofilm culture storage device according to claim 1, characterized in that: The outer wall of the culture storage box (1) is provided with a connecting strip (2), and an insertion hole (3) is provided inside the connecting strip (2).

3. The anaerobic ammonium oxidation biofilm culture storage device according to claim 1, characterized in that: A sealing cover (9) is provided above the culture storage box (1), and an insertion rod (10) is fixed to the outer wall of the sealing cover (9).

4. The anaerobic ammonium oxidation biofilm culture storage device according to claim 3, characterized in that: The number of the insertion rods (10) is consistent with the number of the insertion holes (3), and the insertion rods (10) and the insertion holes (3) are snap-fittedly connected.

5. The anaerobic ammonium oxidation biofilm culture storage device according to claim 3, characterized in that: Temperature controllers (11) are provided on the left and right sides of the upper end of the sealing cover (9), and an air extraction pump (12) is provided in the middle of the upper end of the sealing cover (9), and an air outlet (13) is provided at the upper end of the air extraction pump (12).

6. The anaerobic ammonium oxidation biofilm culture storage device according to claim 3, characterized in that: Heating pipes (14) are installed at the left and right ends of the inner wall of the sealing cover (9), and an air extraction port (15) is installed in the middle of the inner wall of the sealing cover (9).

Citation Information

Patent Citations

  • A rapid enrichment culture device for anammox bacteria

    CN105925478B