Cleaning device for nucleic acid extraction

Abstract

The utility model relates to a cleaning device for nucleic acid extraction, which comprises a turntable, a plurality of sample placing points, a plurality of suction head placing points and at least one waste liquid discharging point, the turntable is provided with a plurality of sample placing points, a plurality of suction head placing points and at least one waste liquid discharging point along the circumferential direction of the turntable, the sample placing points are used for placing sample tubes, and suction heads are placed at the suction head placing points; each liquid treatment mechanism comprises a grabbing mechanism, and the grabbing mechanism is used for driving the suction heads on the suction head placement point positions to move to the corresponding sample placement point positions so as to suck waste liquid in the corresponding sample tubes through the suction heads, and driving the suction heads to move to the waste liquid discharge point positions through the grabbing mechanisms so as to discharge the waste liquid in the suction heads; the at least one liquid treatment mechanism comprises a liquid injection mechanism, and the liquid injection mechanism is used for adding cleaning liquid into the sample tube located at the corresponding sample placement point position. The nucleic acid cleaning device can solve the technical problems that a nucleic acid cleaning part in the nucleic acid extractor is complex and tedious in structure and movement mode and poor in use effect.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction, in particular to a cleaning device for nucleic acid extraction. Background Technique

[0002] Nucleic acids are divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is mainly concentrated in the cell nucleus (in mitochondria and chloroplasts), while RNA is mainly distributed in the cytoplasm. As the material basis of gene expression, nucleic acids are the main objects of molecular biology research. Whether studying the structure or function of nucleic acids, it is first necessary to release the nucleic acids in the sample to be tested from the cells and further separate the released nucleic acids from other impurities in the cells to obtain purer nucleic acids. This process is called nucleic acid extraction and purification. Nucleic acid extraction provides a basis for a large number of extensive studies and applications.

[0003] The nucleic acid extraction methods are mainly divided into three categories: solution extraction method, column extraction method, and magnetic bead purification method. Currently, the commonly used method is the magnetic bead purification method. Among them, the steps of the magnetic bead purification method are as Figure 1 shown. Add lysis buffer to the sample tube containing the sample to release the nucleic acids in the cell nucleus; then add magnetic beads to the sample tube so that the nucleic acids are adsorbed on the magnetic beads, and the magnetic beads adsorbed with nucleic acids are enriched on one side of the sample tube by a magnet; then, after removing the waste liquid in the sample tube, add cleaning solution to wash the nucleic acids; then, after removing the waste liquid after washing the nucleic acids, add elution buffer to separate the nucleic acids from the magnetic beads; finally, remove the magnetic beads to obtain purified nucleic acids. The whole process can be simplified as: lysis, cleaning, and elution.

[0004] At the present stage, many types of nucleic acid extractors have been developed based on the principle of the magnetic bead purification method for nucleic acid extraction and purification of samples to be tested. Among them, the types of nucleic acid extractors include multi-sample synchronous extraction type, single-sample individual extraction type, and multi-sample arrival-and-extraction type. The multi-sample synchronous extraction type of nucleic acid extractor can complete the nucleic acid extraction of multiple samples at one time, but it needs to collect a sufficient number of samples before starting to run and cannot achieve arrival-and-inspection. The single-sample individual extraction type of nucleic acid extractor, although it does not need to collect a sufficient number of samples, has limited processing capacity in the face of a large number of samples and cannot quickly complete nucleic acid extraction. The multi-sample arrival-and-extraction type of nucleic acid extractor can, on the one hand, achieve arrival-and-extraction of samples without waiting to collect a sufficient number of samples, and on the other hand, can also adapt to the situation of a large number of samples and has good development prospects. However, the existing arrival-and-extraction type of nucleic acid extractor technology is not yet mature and has many problems (such as: the rationality of the structure design of the nucleic acid cleaning part in the device is poor, resulting in cumbersome movement of the turntable during nucleic acid cleaning and requiring reciprocating rotation; high requirements for movement accuracy, complex control programs, and easy to cause cross-contamination, etc.), and the actual use effect is not good.

[0005] In the related art, there is no effective solution to the problem that the nucleic acid cleaning part structure and movement mode in the nucleic acid extractor are complex and cumbersome, and the use effect is not good.

[0006] Therefore, based on the experience and practice of being engaged in the relevant industry for many years, the present inventor proposes a cleaning device for nucleic acid extraction to overcome the defects of the prior art. Summary of the Utility Model

[0007] The purpose of the present utility model is to provide a cleaning device for nucleic acid extraction, which has a simple structure and is convenient to operate. Only by controlling the turntable to rotate in a predetermined direction, the nucleic acid cleaning operation of multiple samples that have completed the lysis process can be realized, effectively removing impurities other than nucleic acid, and the actual cleaning effect is good.

[0008] The purpose of the present utility model can be achieved by the following solutions:

[0009] The present utility model provides a cleaning device for nucleic acid extraction, and the cleaning device for nucleic acid extraction includes:

[0010] A turntable, on which a plurality of sample placement points, a plurality of pipette tip placement points and at least one waste liquid discharge point are arranged along its circumference. The plurality of sample placement points are respectively used for placing sample tubes, and a pipette tip is placed at each of the plurality of pipette tip placement points. The sample tubes and the pipette tips rotate synchronously with the turntable;

[0011] A plurality of liquid processing mechanisms,

[0012] Each of the liquid processing mechanisms includes a grasping mechanism, and the grasping mechanism is used to drive the pipette tip at the pipette tip placement point to move to the corresponding sample placement point, so as to suck the waste liquid in the corresponding sample tube through the pipette tip, and drive the pipette tip to move to the waste liquid discharge point through the grasping mechanism, and discharge the waste liquid in the pipette tip to the waste liquid discharge point;

[0013] At least one of the liquid processing mechanisms includes a liquid injection mechanism, and the liquid injection mechanism is used to add cleaning liquid into the sample tube located at the corresponding sample placement point.

[0014] In a preferred embodiment of the present utility model, a plurality of liquid processing working positions are arranged at intervals along the circumference of the turntable, and the plurality of liquid processing mechanisms are arranged on the periphery of the turntable, and in the circumferential direction of the turntable, the plurality of liquid processing mechanisms and the plurality of liquid processing working positions are in one-to-one correspondence.

[0015] In a preferred embodiment of the present utility model, a sample injection working position and a sample output working position are further provided on the turntable. The sample injection working position and the sample output working position are adjacent to each other, and the sample injection working position and the sample output working position are located between two adjacent liquid treatment working positions. Any sample placement point and pipette tip placement point on the turntable start to rotate from the sample injection working position, sequentially pass through a plurality of liquid treatment working positions, and stop rotating at the sample output working position.

[0016] In a preferred embodiment of the present utility model, along the rotation direction of the turntable, at least one liquid treatment mechanism located downstream of the sample injection working position and close to the sample injection working position includes a liquid injection mechanism.

[0017] In a preferred embodiment of the present utility model, in the circumferential direction of the turntable, a plurality of waste liquid discharge points correspond to a plurality of liquid treatment working positions one by one.

[0018] In a preferred embodiment of the present utility model, the waste liquid discharge point is a waste liquid discharge hole opened on the turntable, and the waste liquid discharge hole is connected to a waste liquid recovery device through a recovery pipeline.

[0019] In a preferred embodiment of the present utility model, a plurality of sample placement points correspond to a plurality of pipette tip placement points one by one, and the corresponding sample placement point and pipette tip placement point are located on the same radial extension line of the turntable.

[0020] In a preferred embodiment of the present utility model, the grasping mechanism includes a grasping head in a spherical or cylindrical shape. The top of the pipette tip has a clamping groove, and the radius of the clamping groove is smaller than the radius of the grasping head, so that the grasping head can be press-fitted into the clamping groove to connect the grasping head with the pipette tip.

[0021] In a preferred embodiment of the present utility model, the liquid injection mechanism includes a liquid injection syringe. The liquid injection syringe is connected to a liquid storage device storing cleaning liquid through a liquid injection pipeline. A liquid injection pump is provided on the liquid injection pipeline or the liquid storage device to add the cleaning liquid in the liquid storage device to the sample tube sequentially through the liquid injection pipeline and the liquid injection syringe.

[0022] In a preferred embodiment of the present utility model, the sample placement point and the pipette tip placement point are respectively placement holes opened on the turntable, and the sample tube and the pipette tip are placed in the corresponding placement holes.

[0023] As described above, the features and advantages of the cleaning device for nucleic acid extraction of the present utility model are:

[0024] A plurality of sample placement points, a plurality of pipette tip placement points, and at least one waste liquid discharge point are circumferentially arranged on a rotatable turntable. Multiple sample placement points can respectively place sample tubes, and pipette tips are respectively placed at the pipette tip placement points. The sample tubes and pipette tips can rotate synchronously with the turntable. During the rotation of the turntable, the pipette tip at the corresponding position can be connected through the grasping mechanism in the liquid handling mechanism. The grasping mechanism drives the pipette tip originally at the pipette tip placement point to move to the corresponding sample placement point, and then the pipette tip sucks the waste liquid in the corresponding sample tube. After that, the grasping mechanism drives the pipette tip with the sucked waste liquid to move to the waste liquid discharge point, and discharges the waste liquid in the pipette tip to the waste liquid discharge point. After discharging the waste liquid in the sample tube, the liquid injection mechanism in the liquid handling mechanism can add cleaning liquid to the sample tube at the corresponding sample placement point, so as to clean (wash) the nucleic acid from which the waste liquid has been removed. Since a plurality of liquid handling mechanisms are arranged along the circumference of the turntable, the sample tubes rotating synchronously with the turntable can be subjected to impurity removal and cleaning treatment through multiple liquid handling mechanisms during the rotation process, and the nucleic acid cleaning operation of multiple samples that have completed the lysis treatment can be realized.

[0025] In addition, the cleaning device for nucleic acid extraction of the present invention has a simple structure and convenient operation. Only by controlling the turntable to rotate in a predetermined direction, the nucleic acid cleaning operation of multiple samples that have completed the lysis treatment can be realized, ensuring that impurities other than nucleic acid are fully removed, and the actual cleaning effect is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The following drawings are only intended to illustrate and explain the present invention schematically, and do not limit the scope of the present invention. Among them:

[0027] Figure 1 : is the operation flow chart of the magnetic bead purification method in the prior art;

[0028] Figure 2 : is one of the perspective views of the cleaning device for nucleic acid extraction of the present invention;

[0029] Figure 3 : is the top view of the cleaning device for nucleic acid extraction of the present invention;

[0030] Figure 4 : is the other perspective view of the cleaning device for nucleic acid extraction of the present invention;

[0031] The reference numerals in the present invention are:

[0032] 1, turntable; 101, sample placement point;

[0033] 102, pipette tip placement point; 103, waste liquid discharge point;

[0034] 104. Sampling working position; 105. Sample output working position;

[0035] 106. Liquid handling working position; 1061. First working position;

[0036] 1062. Second working position; 1063. Third working position;

[0037] 1064. Fourth working position; 1065. Fifth working position;

[0038] 1066. Sixth working position; 2. Liquid handling mechanism;

[0039] 201. Gripping mechanism; 202. Liquid injection mechanism;

[0040] 203. First driving mechanism; 204. Second driving mechanism;

[0041] 3. Pipette tip; 4. Outer shell;

[0042] 5. Bottom plate; 501. Extension part. Detailed implementation manners

[0043] For a clearer understanding of the technical features, objectives, and effects of the present utility model, the specific implementation manners of the present utility model will now be described with reference to the accompanying drawings.

[0044] As Figures 2 to 4 shown, the present utility model provides a cleaning device for nucleic acid extraction, which can be used in the nucleic acid purification process to remove the waste liquid added to the sample tube and wash the extracted nucleic acid. Among them, the waste liquid includes the lysis solution after sample lysis and the cleaning solution for washing the magnetic beads from which nucleic acid has been separated.

[0045] The cleaning device for nucleic acid extraction of the present utility model includes a circular turntable 1 and a plurality of liquid handling mechanisms 2. The turntable 1 is rotatably arranged in the horizontal direction. Along the circumference of the turntable 1, a plurality of sample placement points 101, a plurality of pipette tip placement points 102, and at least one waste liquid discharge point 103 are provided. The plurality of sample placement points 101 are respectively used for placing sample tubes, and the plurality of pipette tip placement points 102 are respectively provided with pipette tips 3. The plurality of sample placement points 101 and the plurality of pipette tip placement points 102 are in one-to-one correspondence, that is, one sample tube is equipped with one pipette tip 3, and both the sample tube and the pipette tip 3 can rotate synchronously with the turntable 1.

[0046] Among them, the sample placement point 101 and the pipette tip placement point 102 are respectively placement holes opened on the turntable 1, and the sample tube and the pipette tip 3 can be placed in the corresponding placement holes to fix the sample tube and the pipette tip 3.

[0047] In an alternative embodiment of the present utility model, as Figures 2 to 4 shown, each liquid processing mechanism 2 includes a grasping mechanism 201. The grasping mechanism 201 is used to drive the pipette 3 at the pipette placement point 102 to move to the corresponding sample placement point 101, so as to aspirate the waste liquid in the corresponding sample tube through the pipette 3, and drive the pipette 3 to move to the waste liquid discharge point 103 through the grasping mechanism 201, and discharge the waste liquid in the pipette 3 to the waste liquid discharge point 103; in addition, at least one liquid processing mechanism 2 further includes a liquid injection mechanism 202. The liquid injection mechanism 202 is used to add a cleaning solution into the sample tube located at the corresponding sample placement point 101 to wash the separated nucleic acid.

[0048] In the present utility model, a plurality of sample placement points 101, a plurality of pipette placement points 102 and at least one waste liquid discharge point 103 are arranged along the circumference of the rotatable turntable 1. The plurality of sample placement points 101 can respectively place sample tubes, and pipettes 3 are respectively placed at the pipette placement points 102. The sample tubes and the pipettes 3 can rotate synchronously with the turntable 1; during the rotation of the turntable 1, the grasping mechanism 201 in the liquid processing mechanism 2 can connect to the pipette 3 rotated to its corresponding position, drive the pipette 3 originally located at the pipette placement point 102 to move to the corresponding sample placement point 101 through the grasping mechanism 201, and then aspirate the waste liquid in the corresponding sample tube through the pipette 3. After that, drive the pipette 3 aspirating the waste liquid to move to the waste liquid discharge point 103 through the grasping mechanism 201, and discharge the waste liquid in the pipette 3 to the waste liquid discharge point 103; after discharging the waste liquid in the sample tube, the liquid injection mechanism 202 in the liquid processing mechanism 2 can add a cleaning solution into the sample tube located at the corresponding sample placement point 101, so as to perform a cleaning (washing) operation on the nucleic acid from which the waste liquid has been removed. Since a plurality of liquid processing mechanisms 2 are arranged along the circumference of the turntable 1, the sample tubes rotating synchronously with the turntable 1 can be successively subjected to impurity removal and cleaning treatments by the plurality of liquid processing mechanisms 2 during the rotation process, and the nucleic acid cleaning operation of a plurality of samples that have completed the lysis treatment can be realized.

[0049] The cleaning solution adopted in the present utility model is the cleaning solution used for cleaning the magnetic beads adsorbed with nucleic acid in the existing magnetic bead purification method, and the specific components or types of the cleaning solution are not limited herein.

[0050] The cleaning device for nucleic acid extraction of the present utility model has a simple structure and convenient operation. Only by controlling the turntable 1 to rotate one circle in a predetermined direction (that is, only by rotating in the clockwise direction or the counterclockwise direction all the time, without rotating back and forth for multiple circles), the nucleic acid cleaning operation of a plurality of samples that have completed the lysis treatment can be realized, ensuring that impurities other than nucleic acid are fully removed, and the actual cleaning effect is good.

[0051] In the present utility model, since one sample tube is equipped with one pipette tip 3, and one pipette tip 3 can only suck and discharge the waste liquid in one corresponding sample tube, it is possible to avoid the risk of liquid cross - contamination in different sample tubes that may be caused by using one pipette tip 3 in cooperation with multiple sample tubes.

[0052] In an alternative embodiment of the present utility model, as Figures 2 to 4 shown, a plurality of liquid - processing working positions 106 are arranged at intervals along the circumferential direction on the top surface of the turntable 1. A plurality of liquid - processing mechanisms 2 are arranged on the periphery of the turntable 1, and in the circumferential direction of the turntable 1, the plurality of liquid - processing mechanisms 2 and the plurality of liquid - processing working positions 106 are in one - to - one correspondence (that is: the plurality of liquid - processing mechanisms 2 and the corresponding liquid - processing working positions 106 are located on the same radial extension line of the turntable 1 or are arranged close to the same radial extension line of the turntable 1). When the sample placement position 101 and the corresponding pipette - tip placement position 102 on the turntable 1 are successively rotated to each liquid - processing working position 106, the corresponding plurality of liquid - processing mechanisms 2 can respectively perform a liquid - sucking operation (that is, sucking the waste liquid in the sample tube) and a liquid - injecting operation (that is, injecting cleaning liquid into the sample tube) on the sample tube located below it, so as to ensure the full suction of the waste liquid in the sample tube and the full washing of the nucleic acid. The number of liquid - processing working positions 106 can be set according to the actual washing situation of the nucleic acid. It is possible to inject the cleaning liquid into the sample tube only once and wash the nucleic acid once. Of course, it is also possible to perform two, three or more washes.

[0053] Furthermore, as Figures 2 to 4 shown, an injection working position 104 and an ejection working position 105 are also arranged on the turntable 1. The injection working position 104 and the ejection working position 105 are adjacent to each other, and the injection working position 104 and the ejection working position 105 are located between two adjacent liquid - processing working positions 106. Among them, the liquid - processing working position 106 on the side close to the injection working position 104 is the starting liquid - processing working position for purifying the nucleic acid in the sample tube, and the liquid - processing working position 106 on the side close to the ejection working position 105 is the terminating liquid - processing working position for purifying the nucleic acid in the sample tube.

[0054] During the operation, any sample placement point 101 and pipette tip placement point 102 on the turntable 1 start to rotate from the sample injection working position 104. After passing through multiple liquid handling working positions 106 in sequence, they stop rotating at the sample output working position 105. Specifically, at the sample injection working position 104, a sample tube containing the liquid after lysis treatment is placed at the corresponding sample placement point 101, and at the same time, the pipette tip 3 matching the sample tube is placed at the corresponding pipette tip placement point 102. Then, the sample tube and the pipette tip 3 rotate away from the sample injection working position 104 with the turntable 1. Subsequently, the next sample tube and pipette tip 3 are again placed at another sample placement point 101 and pipette tip placement point 102 at the sample injection working position 104. As the sample tube and the pipette tip 3 rotating with the turntable 1 pass through multiple liquid handling working positions 106 in sequence, after reaching each liquid handling working position 106, the liquid handling mechanism 2 at the corresponding position connects to the pipette tip 3 to aspirate the waste liquid in the corresponding sample tube, and then the gripping mechanism 201 drives the pipette tip 3 with the aspirated waste liquid to move to the waste liquid discharge point 103 to discharge the waste liquid in the pipette tip 3 to the waste liquid discharge point 103. After discharging the waste liquid in the sample tube, the liquid injection mechanism 202 in the liquid handling mechanism 2 adds a cleaning solution to the sample tube located at the corresponding sample placement point 101, so as to perform a cleaning (washing) operation on the nucleic acid from which the waste liquid has been removed. Finally, the sample tube and the pipette tip 3 rotate with the turntable 1 to the sample output working position 105, and at the sample output working position 105, the sample tube containing the sample with the nucleic acid cleaning completed and the pipette tip 3 matching it are taken out of the turntable 1 to complete the cleaning of the nucleic acid.

[0055] In the present utility model, the sample injection working position 104, the sample output working position 105, and multiple liquid handling working positions 106 are all working positions preset on the turntable 1, which are respectively used to calibrate the placement position, the removal position of the sample tube, and the position for washing the nucleic acid in the sample tube and aspirating the waste liquid, so as to accurately display the position for nucleic acid processing during the rotation of the turntable 1. Among them, the larger the distance between two adjacent liquid handling working positions 106, the better, to ensure that the cleaning solution can fully clean the nucleic acid and the waste liquid can be fully aspirated. In the present utility model, it is preferably that any two adjacent liquid handling working positions 106 have the same circumferential distance (that is, multiple liquid handling working positions 106 are evenly and spaced along the axial direction of the turntable 1).

[0056] In an alternative embodiment of the present utility model, as Figures 2 to 4As shown in the figure, along the rotation direction of the turntable 1, at least one liquid handling mechanism 2 located downstream of the sample injection working position 104 and close to the sample injection working position 104 includes a liquid injection mechanism 202. The cleaning liquid is added into the sample tube located at the corresponding sample placement point 101 through the liquid injection mechanism 202 to wash the separated nucleic acid. And at least one liquid handling mechanism 2 located upstream of the sample output working position 105 and close to the sample output working position 105 may only include a grasping mechanism 201. After the nucleic acid in the sample tube is fully washed and no further washing is required, only the waste liquid in the sample tube is sucked out by the cooperation of the grasping mechanism 201 and the pipette tip 3, ensuring that there is no waste liquid residue in the sample tube.

[0057] In an alternative embodiment of the present invention, as Figures 2 to 4 shown, in the circumferential direction of the turntable 1, a plurality of waste liquid discharge points 103 correspond to a plurality of liquid handling working positions 106 one by one. That is, the plurality of waste liquid discharge points 103 and the corresponding liquid handling working positions 106 can be arranged on the same radial extension line of the turntable 1, so that after the pipette tip 3 sucks out the waste liquid, it can move the waste liquid to the liquid handling working position 106 along the radial direction of the turntable 1. During this process, the grasping mechanism 201 only needs to drive the pipette tip 3 to move in the vertical direction and along the radial direction of the turntable 1, without the need for other-dimensional movement, simplifying the movement mode and helping to simplify the structure of the cleaning device.

[0058] Further, the waste liquid discharge point 103 is a waste liquid discharge hole opened on the turntable 1. The waste liquid discharge hole is connected to a waste liquid recovery device through a recovery pipeline. The waste liquid discharge hole is used to collect waste liquid or allow the waste liquid to flow through the waste liquid discharge hole to the waste liquid recovery device for waste liquid recovery. Among them, the waste liquid recovery device can be, but is not limited to, a waste liquid bucket. In an alternative embodiment of the present invention, as Figures 2 to 4 shown, in the radial direction of the turntable 1, the waste liquid discharge point 103 is located inside the sample placement point 101 and the pipette tip placement point 102 (that is: the waste liquid discharge point 103 is closer to the center of the turntable 1 compared to the sample placement point 101 and the pipette tip placement point 102). Of course, according to the actual setting position requirements, the waste liquid discharge point 103 can also be set outside the sample placement point 101 and the pipette tip placement point 102 (that is: the waste liquid discharge point 103 is farther from the center of the turntable 1 compared to the sample placement point 101 and the pipette tip placement point 102), or the waste liquid discharge point 103 can be set at other positions on the top of the turntable 1.

[0059] In an alternative embodiment of the present invention, as Figures 2 to 4As shown, a plurality of sample placement points 101 correspond one-to-one with a plurality of pipette tip placement points 102, and the corresponding sample placement point 101 and pipette tip placement point 102 are located on the same radial extension line of the turntable 1. This facilitates the grasping mechanism 201 to drive the pipette tip 3 to move to the sample tube at the corresponding sample placement point 101 after being connected to the pipette tip 3, facilitating the suction of waste liquid; and it also facilitates the liquid injection mechanism 202 to move to the sample tube at the corresponding sample placement point 101 to facilitate the injection of cleaning liquid into the sample tube. Among them, the distance between the pipette tip placement point 102 and the center point of the turntable 1 is greater than the distance between the corresponding sample placement point 101 and the center point of the turntable 1. Of course, the pipette tip placement point 102 and the corresponding sample placement point 101 can also be replaced in position, as long as the pipette tip placement point 102 and the corresponding sample placement point 101 are located on the same radial extension line of the turntable 1, and the relative position between the two can be adjusted.

[0060] In an alternative embodiment of the present invention, the grasping mechanism 201 includes a grasping head (not shown) in a spherical or cylindrical shape. The pipette tip 3 can be a tubular structure, and the top of the pipette tip 3 has a card slot, and the radius of the card slot is smaller than the radius of the grasping head. Since the pipette tip 3 can be made of a material with a certain elasticity, during the process of the grasping head moving downward in the vertical direction, the grasping head can be press-fitted into the card slot, so that the grasping head is connected to the pipette tip 3, that is, the grasping mechanism 201 grasps the pipette tip 3, and thus the pipette tip 3 can be driven to move. Of course, in the present invention, the specific shape or structure of the grasping head is not limited, as long as it can cooperate with the card slot on the pipette tip 3 for clamping.

[0061] In a specific embodiment of the present invention, the liquid processing mechanism 2 further includes a first driving mechanism 203 that drives the grasping mechanism 201 to move along the vertical direction and the radial direction of the turntable 1 respectively. Among them, the first driving mechanism 203 includes a first driving cylinder with a piston rod that can extend and retract in the vertical direction and a second driving cylinder with a piston rod that can extend and retract in the radial direction of the turntable 1. The cylinder body of the first driving cylinder is fixed to the periphery of the turntable 1, the cylinder body of the second driving cylinder is connected to the piston rod of the first driving cylinder, and the grasping mechanism 201 is connected to the piston rod of the second driving cylinder. The first driving cylinder can drive the grasping mechanism 201 to perform a lifting motion in the vertical direction, and the second driving cylinder can drive the grasping mechanism 201 to perform a translational motion in the radial direction of the turntable 1, so as to drive the grasping mechanism 201 to move to the pipette tip placement point 102 to grasp the pipette tip 3, and drive the pipette tip 3 to move between the pipette tip placement point 102, the corresponding sample placement point 101, and the waste liquid discharge point 103 to complete the suction and discharge of the waste liquid.

[0062] In another specific embodiment of the present utility model, the liquid processing mechanism 2 further includes a first driving mechanism 203 that can at least drive the grasping mechanism 201 to move along the vertical direction and the radial direction of the turntable 1. Among them, the first driving mechanism 203 includes a robotic arm that can perform multi-dimensional movement (such as an existing multi-axis robotic arm). At this time, the first driving mechanism 203 can perform more dimensional movements, not limited to the vertical direction, the radial direction, and the radial movement along the turntable 1, so that the relative positions of the sample placement point 101, the pipette tip placement point 102, and the waste liquid discharge point 103 are no longer limited to the same radial extension line of the turntable 1, but there can be multiple position options. For example, the sample placement point 101, the pipette tip placement point 102, and the waste liquid discharge point 103 can be set at any position on the top surface of the turntable 1 (the three can be on the same fold line, the same arc, or other paths). Of course, the sample placement point 101, the pipette tip placement point 102, and the waste liquid discharge point 103 can also be located outside the turntable 1 instead of on the turntable 1. When the turntable 1 stops rotating, the grasping mechanism 201 first grabs the pipette tip 3 from the pipette tip placement point 102, and then the grasping mechanism 201 drives the pipette tip 3 to move above the sample placement point 101, drives the pipette tip 3 to descend, and the pipette tip 3 extends into the sample tube from the top opening of the sample tube to suck out the waste liquid. Subsequently, the grasping mechanism 201 drives the pipette tip 3 to move above the waste liquid discharge point 103, and after the pipette tip 3 discharges the waste liquid to the waste liquid discharge point 103, it moves to the pipette tip placement point 102 and places the pipette tip 3 back at the pipette tip placement point 102 for the next use.

[0063] In an alternative embodiment of the present utility model, the liquid injection mechanism 202 includes a liquid injection needle tube, and the liquid injection needle tube is connected to a liquid storage device storing cleaning liquid through a liquid injection pipeline. A liquid injection pump is provided on the liquid injection pipeline or the liquid storage device, and the cleaning liquid in the liquid storage device can be pumped and added into the sample tube through the liquid injection pipeline and the liquid injection needle tube in sequence by the liquid injection pump.

[0064] In a specific embodiment of the present utility model, the liquid processing mechanism 2 further includes a second driving mechanism 204 that can at least drive the liquid injection mechanism 202 to move along the vertical direction. Among them, the second driving mechanism 204 includes a third driving cylinder whose piston rod can extend and retract along the vertical direction. The cylinder body of the third driving cylinder is fixed to the periphery of the turntable 1, and the liquid injection mechanism 202 is connected to the piston rod of the third driving cylinder. The third driving cylinder can drive the liquid injection mechanism 202 to perform a lifting movement along the vertical direction, so that the cleaning liquid can be added into the sample tube located at the sample placement point 101 through the liquid injection mechanism 202.

[0065] In an alternative embodiment of the present utility model, the pipette tip 3 can be connected to a pressure regulating device (not shown) through a suction pipeline to regulate the pressure in the inner cavity of the pipette tip 3 through the pressure regulating device, so as to achieve the suction and external discharge of the waste liquid. Among them, the pressure regulating device can be, but is not limited to, a pressure regulating pump.

[0066] In an alternative embodiment of the present utility model, a plurality of marking parts are provided on the turntable 1, and the plurality of marking parts respectively correspond one-to-one to a plurality of sample placement points 101 and a plurality of pipette tip placement points 102. As Figures 2 to 4 shown, the plurality of marking parts may be numbers sequentially arranged along the circumferential direction of the turntable 1 on the top surface of the turntable 1 (such as Figure 3 , Figure 4 the 1 - 72 in), the number of the marking parts is the same as the number of the sample placement points 101 and the pipette tip placement points 102. When performing nucleic acid extraction operations, the operator can directly know the number of sample tubes that can be placed on the turntable 1 through the number of the marking parts, so as to know the number of samples that can be processed simultaneously by the cleaning device for nucleic acid extraction of the present utility model.

[0067] In an alternative embodiment of the present utility model, as Figure 2 shown, the cleaning device for nucleic acid extraction further includes a housing 4 and a bottom plate 5. The bottom plate 5 is arranged in the horizontal direction, the housing 4 is arranged on the top surface of the bottom plate 5, the turntable 1 is arranged in the horizontal direction on the top of the housing 4, and a driving device (not shown) for driving the turntable 1 to rotate is arranged in the housing 4. The driving end of the driving device is connected to the central position of the turntable 1. By driving the turntable 1 to rotate through the driving device, the rotation state and the rotation position of the turntable 1 can be controlled. Among them, the driving device may be, but is not limited to, a driving motor.

[0068] Furthermore, as Figures 2 to 4 shown, a plurality of extension parts 501 extend outward from the bottom plate 5 to its outside. The extension parts 501 are distributed at intervals along the circumferential direction of the bottom plate 5. The plurality of extension parts 501 correspond one-to-one to the plurality of liquid processing mechanisms 2. The liquid processing mechanisms 2 are arranged on the tops of the corresponding extension parts 501 to play a role in supporting the liquid processing mechanisms 2.

[0069] The process of cleaning the sample by the present utility model is as follows:

[0070] As Figure 3 , Figure 4As shown, place the sample tube at the sample placement point 101 corresponding to the sample injection working position 104, and place the pipette tip 3 at the pipette tip placement point 102 that is radially corresponding to the sample injection working position 104 (i.e., on the same radial extension line of the turntable 1). Then, control the turntable 1 to rotate clockwise. When the sample tube and the pipette tip 3 at the sample injection working position 104 rotate to the first working position 1061 in the liquid handling working position 106, they are exactly below the grasping mechanism 201, and the sample placement point 101, the pipette tip placement point 102, and the waste liquid discharge point 103 at the first working position 1061 are on the same radius of the turntable 1. After reaching the first working position 1061, control the turntable 1 to stop rotating. The grasping mechanism 201 moves downward to grasp the pipette tip 3 at the pipette tip placement point 102. Subsequently, the grasping mechanism 201 drives the pipette tip 3 to move towards the sample placement point 101, and then sucks the liquid (waste liquid) in the sample tube at the sample placement point 101 through the pipette tip 3. Then, the grasping mechanism 201 drives the pipette tip 3 to move towards the waste liquid discharge point 103 and discharges the sucked waste liquid to the waste liquid discharge point 103, and the waste liquid can flow out through the waste liquid hole. Subsequently, the grasping mechanism 201 takes the pipette tip 3 and moves it towards the pipette tip placement point 102, and then removes the pipette tip 3 (manually or mechanically, not limited) to the pipette tip placement point 102; then, control the turntable 1 to continue rotating clockwise. When the sample tube and the pipette tip 3 rotate below the liquid injection mechanism 202, control the turntable 1 to stop rotating. At this time, inject the cleaning liquid into the sample tube at the sample placement point 101 through the liquid injection mechanism 202; then, control the turntable 1 to continue rotating clockwise. When the turntable 1 rotates to the second working position 1062 in the liquid handling working position 106, perform the same operations as those in the aforementioned first working position 1061 (i.e., suck waste liquid, discharge waste liquid, inject cleaning liquid) through the grasping mechanism 201 and the liquid injection mechanism 202 at the second working position 1062; then, control the turntable 1 to continue rotating clockwise. When the turntable 1 rotates to the third working position 1063 and the fourth working position 1064 in the liquid handling working position 106 in sequence, perform the same operations as those in the aforementioned first working position 1061 through the grasping mechanism 201 and the liquid injection mechanism 202 at the third working position 1063 and the fourth working position 1064 respectively; when the turntable 1 rotates to the fifth working position 1065 and the sixth working position 1066, since only the grasping mechanism 201 is provided at the fifth working position 1065 and the sixth working position 1066 and the liquid injection mechanism 202 is not provided, only the operations of sucking waste liquid and discharging waste liquid are performed to ensure that the waste liquid can be fully discharged after sufficient cleaning; finally, control the turntable 1 to continue rotating clockwise to the sample output working position 105, and at the sample output working position 105, the sample tube and the pipette tip 3 can be removed from the turntable 1 to complete the cleaning operation of the sample to be tested.

[0071] In the above process, only the operations of sucking and discharging waste liquid are performed at the last two working positions (i.e., the fifth working position 1065 and the sixth working position 1066). The reason for repeating the above operations twice is to suck out the waste liquid in the sample tube completely to ensure the cleaning effect. Of course, finally, only one operation of sucking and discharging waste liquid can be performed alone, or more than two operations of sucking and discharging waste liquid can be performed, as long as it can ensure that the waste liquid is fully sucked out.

[0072] The characteristics and advantages of the cleaning device for nucleic acid extraction of the present utility model are as follows:

[0073] First, in the cleaning device for nucleic acid extraction, during the rotation of the turntable 1, the grasping mechanism 201 in the liquid handling mechanism 2 can connect to the pipette tip 3 rotated to its corresponding position. The grasping mechanism 201 drives the pipette tip 3 originally located at the pipette tip placement point 102 to move to the corresponding sample placement point 101, then the pipette tip 3 sucks the waste liquid in the corresponding sample tube, and then the grasping mechanism 201 drives the pipette tip 3 with the sucked waste liquid to move to the waste liquid discharge point 103, and discharges the waste liquid in the pipette tip 3 to the waste liquid discharge point 103. After discharging the waste liquid in the sample tube, the liquid injection mechanism 202 in the liquid handling mechanism 2 can add cleaning liquid to the sample tube located at the corresponding sample placement point 101, so as to perform the cleaning operation on the nucleic acid from which the waste liquid has been removed. Since a plurality of liquid handling mechanisms 2 are arranged along the circumference of the turntable 1, the sample tubes rotating synchronously with the turntable 1 can be successively subjected to impurity removal and cleaning treatments by the plurality of liquid handling mechanisms 2 during the rotation process, and the nucleic acid cleaning operation of multiple samples that have completed the lysis treatment can be realized, ensuring that impurities other than nucleic acid are fully removed, and the actual cleaning effect is good.

[0074] Second, the cleaning device for nucleic acid extraction has a simple structure and convenient operation. Only by controlling the turntable 1 to rotate in a predetermined direction (i.e., the clockwise direction or the counterclockwise direction), the nucleic acid cleaning operation of multiple samples that have completed the lysis treatment can be realized, without the need to rotate back and forth for multiple circles, the operation is more convenient, and the corresponding control program will also be simpler. Moreover, it helps to extend the service life of the turntable 1.

[0075] Third, in the cleaning device for nucleic acid extraction, when the turntable 1 and the grasping mechanism 201 cooperate to wash the nucleic acid in the sample tube, the grasping mechanism 201 only needs to perform movements in two dimensions, namely the vertical direction and the radial direction along the turntable 1. The functional requirements for the grasping mechanism 201 are low, which can make the grasping mechanism 201 simpler and help to extend the service life of the grasping mechanism 201.

[0076] IV. In the cleaning device for nucleic acid extraction, one sample tube is equipped with one pipette tip 3, and one pipette tip 3 can only suck and discharge the waste liquid in one corresponding sample tube. Therefore, the risk of liquid cross-contamination between different sample tubes caused by using one pipette tip 3 in cooperation with multiple sample tubes can be avoided.

[0077] The above is only a schematic specific embodiment of the present utility model and is not intended to limit the scope of the present utility model. Any equivalent changes and modifications made by those skilled in the art without departing from the concept and principle of the present utility model shall fall within the scope of protection of the present utility model.

Claims

1. A cleaning device for nucleic acid extraction, characterized in that: The cleaning device for nucleic acid extraction comprises: A turntable, wherein a plurality of sample placement points, a plurality of pipette tip placement points and at least one waste liquid discharge point are arranged on the turntable along its circumference, the plurality of sample placement points are respectively used to place sample tubes, the plurality of pipette tip placement points are respectively placed with pipette tips, and the sample tubes and the pipette tips rotate synchronously with the turntable; Multiple liquid handling mechanisms, Each of the liquid handling mechanisms comprises a gripping mechanism, wherein the gripping mechanism is used to drive the pipette tip at the pipette tip placement position to move to the corresponding sample placement position, so as to absorb the waste liquid in the corresponding sample tube through the pipette tip, and to drive the pipette tip to move to the waste liquid discharge position through the gripping mechanism to discharge the waste liquid in the pipette tip; At least one of the liquid handling mechanisms includes a liquid injection mechanism, which is used to add cleaning liquid into the sample tube located at the corresponding sample placement point.

2. The cleaning device for nucleic acid extraction according to claim 1, characterized in that: The turntable is provided with a plurality of liquid processing workstations at intervals along its circumference, the plurality of liquid processing mechanisms are arranged on the periphery of the turntable, and in the circumference of the turntable, the plurality of liquid processing mechanisms correspond one-to-one to the plurality of liquid processing workstations.

3. The cleaning device for nucleic acid extraction according to claim 2, characterized in that: The turntable is also provided with a sample injection workstation and a sample output workstation, wherein the sample injection workstation is adjacent to the sample output workstation and is located between two adjacent liquid processing workstations. Any of the sample placement points and the pipette tip placement points on the turntable starts to rotate from the sample injection workstation, passes through a plurality of the liquid processing workstations in sequence, and stops rotating at the sample output workstation.

4. The cleaning device for nucleic acid extraction according to claim 3, characterized in that: Along the rotation direction of the turntable, at least one of the liquid handling mechanisms located downstream of the sample injection workstation and close to the sample injection workstation includes a liquid injection mechanism.

5. The cleaning device for nucleic acid extraction according to claim 2, characterized in that: In the circumferential direction of the turntable, the plurality of waste liquid discharge points correspond one to one with the plurality of liquid processing work positions.

6. The cleaning device for nucleic acid extraction according to claim 5, characterized in that: The waste liquid discharge point is a waste liquid discharge hole opened on the turntable, and the waste liquid discharge hole is connected to the waste liquid recovery device through a recovery pipeline.

7. The cleaning device for nucleic acid extraction according to any one of claims 1 to 3, characterized in that: The plurality of sample placement points correspond to the plurality of pipette tip placement points one by one, and the corresponding sample placement points and pipette tip placement points are located on the same radial extension line of the turntable.

8. The cleaning device for nucleic acid extraction according to claim 1 or 2, characterized in that: The gripping mechanism comprises a spherical or cylindrical gripping head, the top of the suction head is provided with a slot, the radius of the slot is smaller than the radius of the gripping head, so that the gripping head can be inserted into the slot by interference fit, so that the gripping head is connected to the suction head.

9. The cleaning device for nucleic acid extraction according to claim 1 or 4, characterized in that: The injection mechanism includes an injection needle, which is connected to a liquid storage device storing cleaning liquid through an injection pipeline. An injection pump is provided on the injection pipeline or the liquid storage device to add the cleaning liquid in the liquid storage device into the sample tube in sequence through the injection pipeline and the injection needle.

10. The cleaning device for nucleic acid extraction according to claim 1, characterized in that: The sample placement point and the pipette tip placement point are placement holes respectively opened on the turntable, and the sample tube and the pipette tip are placed in the corresponding placement holes.

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