Stem cell amplification equipment

Abstract

The utility model discloses a stem cell amplification device which comprises a culture assembly, a liquid storage assembly, an amplification assembly and a control assembly, and the culture assembly comprises a culture box, a supporting plate and a 3D culture rotary table; the damage prevention assembly comprises a square through hole, a first fixed plate, a first spring, a first movable plate and a semicircular opening; the square through holes are formed in the side edges of the incubator, the two first fixing plates are fixed to the side edges of the incubator, and one ends of the first springs are fixed to the side edges of the first fixing plates; and the number of the clamping assemblies is two, and each clamping assembly comprises a second fixed plate, a connecting shaft and a second movable plate. The problems that an existing 3D rotary table is usually placed in a culture box to guarantee the culture temperature, but the 3D rotary table needs to be connected with an external controller through a cable, the cable extends out of a transparent inner door and an outer door, the cable is damaged after the door is closed, on the other hand, the transparent inner door and the outer door cannot be completely closed, and due to the fact that the temperature needs to be guaranteed, the temperature cannot be guaranteed are solved. And resource waste is also caused.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an amplification device for stem cells. Background Technique

[0002] Cell culture is a basic and important technique in the laboratory, which involves growing and multiplying cells of animals, plants or microorganisms in an in vitro environment. The amplification device for stem cells is a system or device specifically used for large-scale culture and amplification of stem cells in vitro. The main purpose of this device is to provide a suitable environment for stem cells to grow and multiply rapidly under artificial conditions, so as to meet the needs of clinical and scientific research. The existing 3D culture technique is to place a culture bottle containing stem cells, microcarriers and serum-free medium on a 3D turntable, and the rotating rod in the culture bottle rotates the liquid in the bottle to make the stem cells grow three-dimensionally.

[0003] The existing 3D turntable is usually placed in an incubator to ensure the culture temperature. However, the 3D turntable needs to be connected to an external controller through a cable, and the cable extends out from the transparent inner door and the outer door. After closing the door, the cable will be damaged. On the other hand, the transparent inner door and the outer door cannot be completely closed, which will also cause waste of resources due to the need to ensure the temperature. Therefore, an amplification device for stem cells is needed to solve the above problems. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an amplification device for stem cells to solve the problems put forward in the above background technique, that is, the existing 3D turntable is usually placed in an incubator to ensure the culture temperature. However, the 3D turntable needs to be connected to an external controller through a cable, and the cable extends out from the transparent inner door and the outer door. After closing the door, the cable will be damaged. On the other hand, the transparent inner door and the outer door cannot be completely closed, which will also cause waste of resources due to the need to ensure the temperature.

[0005] To solve the above technical problems, the utility model is realized through the following technical solutions:

[0006] The utility model is an amplification device for stem cells, including:

[0007] A culture component, the culture component includes an incubator, a support plate and a 3D culture turntable;

[0008] The support plate is fixed inside the incubator, and the 3D culture turntable is placed on the support plate;

[0009] An anti-damage component, the anti-damage component includes a square through-hole, a first fixing plate, a first spring, a first movable plate and a semi-circular opening;

[0010] The square through-hole is opened on the side of the incubator. There are two first fixing plates, which are fixed on the side of the incubator and are respectively located on the upper and lower sides of the square through-hole. One end of the first spring is fixed on the side of the first fixing plate, and the first movable plate is fixed on the other end of the first spring. The two first movable plates are in contact with each other, and a semi-circular opening is opened on the side of the first movable plate;

[0011] Clamping assemblies, there are two clamping assemblies in total, including second fixing plates, connecting shafts, and second movable plates;

[0012] There are two second fixing plates, which are fixed on the side of the incubator and are located on the side of the square through-hole. The connecting shaft is fixed between the second fixing plates, and the second movable plate is rotatably connected to the connecting shaft.

[0013] Further, the anti-damage assembly further includes a semi-circular sealing ring and a sealing gasket;

[0014] The semi-circular sealing ring is fixed inside the semi-circular opening, and the sealing gasket is fixed on the side of the first movable plate and is in contact with the incubator.

[0015] Further, the clamping assembly further includes a torsion spring, a connecting plate, and an anti-slip pad;

[0016] The torsion spring is sleeved on the connecting shaft. One end of the connecting plate is sleeved on the connecting shaft, and the other end is fixed on the second movable plate. The anti-slip pad is fixed on the side of the second movable plate and is movably connected to the first movable plate.

[0017] Further, the culture assembly further includes a transparent inner door, an outer door, and an interface;

[0018] The transparent inner door and the outer door are hinged on the side of the incubator, and the outer door is located outside the transparent inner door. The interface is fixed on the side of the 3D incubator.

[0019] Further, a sealing assembly is further included;

[0020] The sealing assembly includes an L-shaped support plate, a second spring, a card slot, a sealing plate, and a limiting plate;

[0021] The L-shaped support plate is fixed on the upper surface of the upper first fixing plate. The upper end of the second spring is fixed on the lower surface of the L-shaped support plate. The card slot is fixed on the side of the first movable plate and is respectively located outside the semi-circular opening. The sealing plate is inserted into the card slot. The limiting plate is fixed on the upper end of the sealing plate, and the upper surface of the sealing plate is fixed on the lower end of the second spring.

[0022] Further, the number of semi-circular openings corresponds to the number of interfaces, and a sealing plate is installed at each semi-circular opening.

[0023] Compared with the prior art, the advantages of the present utility model are:

[0024] 1. For this utility model, through the anti-damage component and clamping component provided, turn the second movable plate with both hands to make the anti-slip pad disengage from the first movable plate, then spread apart the two fitted first movable plates, release the second movable plate, and under the action of the torsion spring, the connecting plate drives the second movable plate and the anti-slip pad to reset. At this time, the two first movable plates are separated, the square through-hole is exposed, the cable is inserted into the incubator through the square through-hole, open the second movable plate according to the above steps, the first movable plates are combined under the action of the first spring, the cable is wrapped in the semi-circular sealing ring with a semi-circular opening, release the second movable plate, and the second movable plate drives the anti-slip pad to press tightly on the first movable plate, so that the sealing pad on the side of the first movable plate is tightly attached to the incubator. This setting can prevent the cable from passing through the transparent inner door and the outer door, causing damage, and at the same time reducing energy waste.

[0025] 2. For this utility model, through the sealing component provided, there are multiple rotating stations on the 3D culture turntable. If not all are used, the cable at this place is not connected. At this time, the second spring below the L-shaped support plate presses the limiting plate, driving the sealing plate to move completely into the card slot. This setting makes the sealing plate close the semi-circular opening to prevent it from being exposed and affecting the temperature inside the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of this utility model, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of this utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0027] Figure 1 It is a schematic diagram of the overall structure of this utility model;

[0028] Figure 2 It is a schematic diagram of the culture component structure of this utility model;

[0029] Figure 3 It is a schematic diagram of the first perspective of the clamping component and the sealing component of this utility model;

[0030] Figure 4 It is a schematic diagram of the second perspective of the clamping component and the sealing component of this utility model.

[0031] In the drawings, the list of components represented by each reference numeral is as follows:

[0032] 10. Incubator; 11. Support plate; 12. Transparent inner door; 13. Outer door; 14. 3D culture turntable; 15. Interface;

[0033] 20. Square through-hole; 21. First fixing plate; 22. First spring; 23. First movable plate; 24. Semi-circular opening; 25. Semi-circular sealing ring; 26. Sealing pad;

[0034] 30. Second fixing plate; 31. Connecting shaft; 32. Torsion spring; 33. Connecting plate; 34. Second movable plate; 35. Anti-slip pad;

[0035] 40. L-shaped support plate; 41. Second spring; 42. Card slot; 43. Sealing plate; 44. Limiting plate. Detailed implementation manners

[0036] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the following will describe the detailed implementation manners of the present utility model in conjunction with the accompanying drawings.

[0037] In the following description, many specific details are set forth to fully understand the present utility model. However, the present utility model can also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the connotation of the present utility model. Therefore, the present utility model is not limited by the specific implementation manners disclosed below.

[0038] To make the purpose, technical solution, and advantages of the present utility model clearer, the following will further describe the implementation manners of the present utility model in detail in conjunction with the accompanying drawings.

[0039] Please refer to Figures 1-4 As shown, this embodiment is an amplification device for stem cells, including:

[0040] A culture component, which includes a culture box 10, a support plate 11, and a 3D culture turntable 14;

[0041] The support plate 11 is fixed inside the culture box 10, and the 3D culture turntable 14 is placed on the support plate 11;

[0042] The culture box 10 can ensure the culture temperature, the support plate 11 plays a supporting role, the 3D culture turntable 14 is used to place the culture bottle, and multiple groups of rotating workstations are provided on the 3D culture turntable 14. Through cables connected to an external controller, the working parameters such as the rotation speed and time of each group of workstations can be controlled separately;

[0043] An anti-damage component, which includes a square through-hole 20, a first fixing plate 21, a first spring 22, a first movable plate 23, and a semi-circular opening 24;

[0044] The square through-hole 20 is opened on the side of the culture box 10. There are two first fixing plates 21, which are fixed on the side of the culture box 10 and are respectively located above and below the square through-hole 20. One end of the first spring 22 is fixed to the side of the first fixing plate 21, and the first movable plate 23 is fixed to the other end of the first spring 22. The two first movable plates 23 are in contact with each other, and the semi-circular opening 24 is opened on the side of the first movable plate 23;

[0045] The square through-hole 20 provides a passage for the cable. The first fixing plate 21 plays a supporting role. The first spring 22 has an extrusion function. The first movable plate 23 plays a closing role. The semi-circular opening 24 facilitates the passage of the cable.

[0046] Clamping assemblies, there are two clamping assemblies, including a second fixing plate 30, a connecting shaft 31, and a second movable plate 34.

[0047] There are two second fixing plates 30, which are fixed on the side of the incubator 10 and are located on the side of the square through-hole 20. The connecting shaft 31 is fixed between the second fixing plates 30. The second movable plate 34 is rotatably connected to the connecting shaft 31.

[0048] The second fixing plate 30 plays a supporting role. The connecting shaft 31 plays a connecting role. The second movable plate 34 plays a supporting role.

[0049] The anti-damage assembly further includes a semi-circular sealing ring 25 and a sealing gasket 26.

[0050] The semi-circular sealing ring 25 is fixed inside the semi-circular opening 24. The sealing gasket 26 is fixed on the side of the first movable plate 23 and fits with the incubator 10.

[0051] The semi-circular sealing ring 25 and the sealing gasket 26 play a sealing role.

[0052] The clamping assembly further includes a torsion spring 32, a connecting plate 33, and an anti-slip pad 35.

[0053] The torsion spring 32 is sleeved on the connecting shaft 31. One end of the connecting plate 33 is sleeved on the connecting shaft 31 and the other end is fixed on the second movable plate 34. The anti-slip pad 35 is fixed on the side of the second movable plate 34 and is movably connected to the first movable plate 23.

[0054] The torsion spring 32 has a reset function. The connecting plate 33 plays a connecting role. The anti-slip pad 35 has an anti-slip effect.

[0055] The culture assembly further includes a transparent inner door 12, an outer door 13, and an interface 15.

[0056] The transparent inner door 12 and the outer door 13 are hinged on the side of the incubator 10, and the outer door 13 is located outside the transparent inner door 12. The interface 15 is fixed on the side of the 3D incubator 10.

[0057] The transparent inner door 12 and the outer door 13 play a closing role. The interface 15 is used to connect the cable to connect to an external controller through the cable.

[0058] Working principle:

[0059] Rotate the second movable plate 34 with both hands to disengage the anti-slip pad 35 from the first movable plate 23, then spread apart the two fitted first movable plates 23, release the second movable plate 34, and under the action of the torsion spring 32, the connecting plate 33 drives the second movable plate 34 and the anti-slip pad 35 to reset;

[0060] At this time, the two first movable plates 23 are separated, and the square through-hole 20 is exposed. The cable is inserted into the incubator 10 through the square through-hole 20. Open the second movable plate 34 according to the above steps. Under the action of the first spring 22, the first movable plates 23 are combined. The cable is wrapped in the semi-circular sealing ring 25 of the semi-circular opening 24. Release the second movable plate 34, and the second movable plate 34 drives the anti-slip pad 35 to press tightly on the first movable plate 23, so that the sealing pad 26 on the side of the first movable plate 23 is closely attached to the incubator 10.

[0061] This step can prevent the cable from passing through the transparent inner door 12 and the outer door 13, causing damage, and at the same time reducing energy waste.

[0062] Please refer to Figures 3-4 , based on the above embodiment, this embodiment further includes:

[0063] A sealing assembly, which includes an L-shaped support plate 40, a second spring 41, a card slot 42, a sealing plate 43, and a limiting plate 44;

[0064] The L-shaped support plate 40 is fixed on the upper surface of the upper first fixing plate 21. The upper end of the second spring 41 is fixed on the lower surface of the L-shaped support plate 40. The card slot 42 is fixed on the side of the first movable plate 23 and is located outside the semi-circular opening 24 respectively. The sealing plate 43 is inserted into the card slot 42. The limiting plate 44 is fixed on the upper end of the sealing plate 43, and the upper surface of the sealing plate 43 is fixed on the lower end of the second spring 41;

[0065] The L-shaped support plate 40 plays a supporting role, the second spring 41 has an extrusion function, the card slot 42 plays a limiting role, the sealing plate 43 plays a sealing role, and the limiting plate 44 plays a limiting role;

[0066] The number of the semi-circular openings 24 corresponds to the number of the interfaces 15, and a sealing plate 43 is installed at each semi-circular opening 24.

[0067] Working principle:

[0068] Multiple rotating stations are provided on the 3D culture turntable 14. If not all are used, the cables at that place are not connected;

[0069] At this time, the second movable plate 34 and the first movable plate 23 are opened in sequence, driving the sealing plate 43 to move upward. Then, the sealing plate 43 is pushed upward along the card slot 42, driving the limiting plate 44 to move upward and compressing the second spring 41. The cable is inserted into the square through hole 20 from the semi-circular opening 24. After releasing the first movable plate 23 and the second movable plate 34, the sealing plate 43 at the semi-circular opening 24 with the inserted cable is blocked by the cable and is located above the cable. At the semi-circular opening 24 without the inserted cable, the sealing plate 43 is completely inserted into the card slot 42 under the action of the second spring 41, closing the semi-circular opening 24.

[0070] This step enables the sealing plate 43 to close the semi-circular opening 24, preventing it from being exposed and affecting the temperature inside the incubator 10.

[0071] In the description of the present utility model, it should also be noted that unless otherwise clearly specified and defined, the terms "set", "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0072] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A stem cell expansion device, characterized in that: include: A culture assembly, the culture assembly comprising a culture box (10), a support plate (11) and a 3D culture turntable (14); The support plate (11) is fixed inside the incubator (10), and the 3D culture turntable (14) is placed on the support plate (11); An anti-damage component, the anti-damage component comprising a square through hole (20), a first fixed plate (21), a first spring (22), a first movable plate (23) and a semicircular opening (24); The square through hole (20) is provided on the side of the incubator (10); there are two first fixed plates (21) fixed on the side of the incubator (10) and respectively located on the upper and lower sides of the square through hole (20); one end of the first spring (22) is fixed on the side of the first fixed plate (21); the first movable plate (23) is fixed on the other end of the first spring (22); the two first movable plates (23) are fitted together; and a semicircular opening (24) is provided on the side of the first movable plate (23); A clamping assembly, wherein the clamping assembly has two components, including a second fixed plate (30), a connecting shaft (31), and a second movable plate (34); The second fixed plates (30) are composed of two pieces, which are fixed on the side of the incubator (10) and located on the side of the square through hole (20). The connecting shaft (31) is fixed between the second fixed plates (30), and the second movable plate (34) is rotatably connected to the connecting shaft (31).

2. A stem cell expansion device according to claim 1, characterized in that: The anti-damage component also includes a semicircular sealing ring (25) and a sealing gasket (26); The semicircular sealing ring (25) is fixed on the inner side of the semicircular opening (24), and the sealing pad (26) is fixed on the side of the first movable plate (23) and is in contact with the incubator (10).

3. The stem cell expansion device according to claim 1, characterized in that: The clamping assembly also includes a torsion spring (32), a connecting plate (33) and an anti-slip pad (35); The torsion spring (32) is sleeved on the connecting shaft (31), one end of the connecting plate (33) is sleeved on the connecting shaft (31), and the other end is fixed on the second movable plate (34), and the anti-slip pad (35) is fixed on the side of the second movable plate (34) and movably connected to the first movable plate (23).

4. The stem cell expansion device according to claim 1, characterized in that: The culture assembly further comprises a transparent inner door (12), an outer door (13) and an interface (15); The transparent inner door (12) and the outer door (13) are hinged on the side of the incubator (10), and the outer door (13) is located outside the transparent inner door (12), and the interface (15) is fixed on the side of the 3D incubator (10).

5. The stem cell expansion device according to claim 1, characterized in that: Also included is a sealing assembly; The sealing assembly comprises an L-shaped supporting plate (40), a second spring (41), a clamping groove (42), a sealing plate (43) and a limiting plate (44); The L-shaped support plate (40) is fixed on the upper surface of the first fixed plate (21), the upper end of the second spring (41) is fixed on the lower surface of the L-shaped support plate (40), the clamping groove (42) is fixed on the side of the first movable plate (23) and is respectively located outside the semicircular opening (24), the sealing plate (43) is inserted into the clamping groove (42), the limiting plate (44) is fixed on the upper end of the sealing plate (43), and the upper surface of the sealing plate (43) is fixed on the lower end of the second spring (41).

6. The stem cell expansion device according to claim 1, characterized in that: The number of the semicircular openings (24) corresponds to the number of the interfaces (15), and a sealing plate (43) is installed at each semicircular opening (24).

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